BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,614,182	T→C	F124L (TTC→CTC)	nikC →	NikC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,614,182	0	T	C	98.7%	491.6 / ‑3.2	153	F124L (TTC→CTC)	nikC	NikC
Reads supporting (aligned to +/- strand): ref base T (2/0); new base C (79/72); total (81/72)
Fisher's exact test for biased strand distribution p-value = 4.98e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.92e-01

Only 100 of 164 total aligned reads displayed.

GGCAGCGCCGGGTTGATTGGCGGGCGCGTTGATCAGGCCACCATGCGCGTCGCCGATATGTTTATGACCTTCCCGACCTCGATTCTGTCGTTCTTTATGGTTGGCGTGCTCGGCACCGGGCTGACCAACGTAATTATCGCCATCGCCCTGTCGCACTGGGCGTGGTATGCACGCATGGT  >  NC_000913/3614092‑3614270

ggcAGCGCCGGGTTGATTGGCGGGCGCGTTGATCAGGCCACCATGCGCGTCGCCGATATGTTTATGACCTTCCCGACCTCGATTCTGTCGCTc                                                                                        <  1:3365885/93‑1 (MQ=255)

  cAGCGCCGGGTTGATTGGCGGGCGCGTTGATCAGGCCACCATGCGCGTCGCCGATATGTTTATGACCTTCCCGCCCTCGATTCTGTCGCTCtt                                                                                      <  1:4023896/93‑1 (MQ=255)

   aGCGCCGGGTTGATTGGCGGGCGCGTTGATCAGGCCACCATGCGCGTCGCCGATATGTTTATGACCTTCCCGACCTCGATTCTGTCGCTCttt                                                                                     >  2:1282662/1‑93 (MQ=255)

   aGCGCCGGGTTGATTGGCGGGCGCGCTGATCAGGCCCCCATGCGCGTCGCCGATATGTTTATTACCTTCCCGACCTCGATTCTGTCGCTCttt                                                                                     >  2:3411531/1‑93 (MQ=255)

     cgcCGGGTTGATTGGCGGGCGCGTTGATCAGGCCACCATGCGCGTCGCCGATATGTTTATGACCTTCCCGACCTCGATTCTGTCGCTCTTTAt                                                                                   <  2:4716048/93‑1 (MQ=255)

      gcCGGGTTGATTGGCGGGCGCGTTGATCAGGCCACCATGCGCGTCGCCGATATGTTTATGACCTTCCCGACCTCGATTCTGTCGCTCTTTATg                                                                                  <  2:2536073/93‑1 (MQ=255)

         gggTTGATTGGCGGGCGCGTTGATCAGGCCACCATGCGCGTCGCCGATATGTTTATGACCTTCCCGACCTCGATTCTGTCGCTCTTTATGGtt                                                                               <  1:411511/93‑1 (MQ=255)

         gggTTGATTGGCGGGCGCGTTGATCAGGCCACCATGCGCGTCGCCGATATGTTTATGACCTTCCCGACCTCGATTCTGTCGCTCTTTATGGtt                                                                               <  2:1231498/93‑1 (MQ=255)

         gggTTGATTGGCGGGCGCGTTGATCAGGCCACCATGCGCGTCGCCGATATGTTTATGACCTTCCCGACCTCGATTCTGTCGCTCTTTATGGtt                                                                               <  1:2050300/93‑1 (MQ=255)

          ggTTGATTGGCGGGCGCGTTGATCAGGCCCCCATGCGCGTCGCCGATATGTTTATGACCTTCCCGACCTCGATTCTGTCGCTCTTTATGGTTg                                                                              <  2:942863/93‑1 (MQ=255)

          ggTTGATTGGCGGGCGCGTTGATCAGGCCACCATGCGCGTCGCCGATATGTTTATGACCTTCCCGACCTCGATTCTGTCGCTCTTTATGGTTg                                                                              <  2:3898997/93‑1 (MQ=255)

          ggTTGATTGGCGGGCGCGTTGATCAGGCCACCATGCGCGTCGCCGATATGTTTATGACCTTCCCGACCTCGATTCTGTCGCTCTTTATGGTTg                                                                              >  2:2934957/1‑93 (MQ=255)

            ttGATTGGCGGGCGCGTTGATCAGGCCACCATGCGCGTCGCCGATATGTTTATGACCTTCCCGACCTCGATTCTGTCGCTCTTTATGGTTGGc                                                                            <  2:1925470/93‑1 (MQ=255)

             tGATTGGCGGGCGCGTTGATCAGGCCACCATGCGCGTCGCCGATATGTTTATGACCTTCCCGACCTCGATTCTGTCGCTCTTTATGGTTGGCg                                                                           >  1:2101270/1‑93 (MQ=255)

             tGATTGGCGGGCGCGTTGATCAGGCCACCATGCGCGTCGCCGATATGTTTATGACCTTCCCGACCTCGATTCTGTCGCTCTTTATGGTTGGCg                                                                           >  1:878542/1‑93 (MQ=255)

              gATTGGCGGGCGCGTTGATCAGGCCACCATGCGCGTCGCCGATATGTTTATGACCTTCCCGACCTCGATTCTGTCGCTCTTTATGGTTGGCGt                                                                          <  2:2218978/93‑1 (MQ=255)

              gATTGGCGGGCGCGTTGATCAGGCCACCATGCGCGTCGCCGATATGTTTATGACCTTCCCGACCTCGATTCTGTCGCTCTTTATGGTTGGCGt                                                                          <  1:4246593/93‑1 (MQ=255)

              gATTGGCGGGCGCGTTGATCAGGCCACCATGCGCGTCGCCGATATGTTTATGACCTTCCCGACCTCGATTCTGTCGCTCTTTATGGTTGGCGt                                                                          <  1:1217397/93‑1 (MQ=255)

               aTTGGCGGGCGCGTTGATCAGGCCACCATGCGCGTCGCCGATATGTTTATGACCTTCCCGACCTCGATTCTGTCGCTCTTTATGGTTGGCGTg                                                                         >  1:4309816/1‑93 (MQ=255)

                 tGGCGGGCGCGTTGATCAGGCCACCATGCGCGTCGCCGATATGTTTATGACCTTCCCGACCTCGATTCTGTCGCTCTTTATGGTTGGCGTGCt                                                                       <  1:1987883/93‑1 (MQ=255)

                 tGGCGGGCGCGTTGATCAGGCCACCATGCGCCTCGCCGATATGTTTATGACCTTCCCGACCTCGATTCGGTCGCTCTTTATGGTTGGCGTGCt                                                                       <  1:140224/93‑1 (MQ=255)

                  ggcgggcgCGTTGATCAGGCCACCATGCGCGTCGCCGATATGTTTATGACCTTCCCGACCTCGATTCTGTCGCTCTTTATGGTTGGCGTGCTc                                                                      <  2:2737161/93‑1 (MQ=255)

                  ggcgggcgCGTTGATCAGGCCACCATGCGCGTCGCCGATATGTTTATGACCTTCCCGACCTCGATTCTGTCGCTCTTTATGGTTGGCGTGCTc                                                                      <  1:1140866/93‑1 (MQ=255)

                   gcgggcgCGTTGATCAGGCCACCATGCGCGTCGCCGATATGTTTATGACCTTCCCGACCTCGATTCTGTCGCTCTTTATGGTTGGCGTGCTCg                                                                     <  2:796085/93‑1 (MQ=255)

                   gcgggcgCGTTGATCAGGCCACCATGCGCGTCGCCGATATGTTTATGACCTTCCCGACCTCGATTCTGTCGCTCTTTATGGTTGGCGTGCTCg                                                                     >  1:4665344/1‑93 (MQ=255)

                    cgggcgCGTTGATCAGGCCACCATGCGCGTCGCCGATATGTTTATGACCTTCCCGACCTCGATTCTGTCGCTCTTTATGGTTGGCGTGCTCgg                                                                    <  2:946711/93‑1 (MQ=255)

                      ggcgCGTTGATCAGGCCACCATGCGCGTCGCCGATATGTTTATGACCTTCCCGACCTCGATTCTGTCGCTCTTTATGGTTGGCGTGCTCGGCa                                                                  >  1:625350/1‑93 (MQ=255)

                         gcgTTGATCAGGCCACCATGCGCGTCGCCGATATGTTTATGACCTTCCCGACCTCGATTCTGTCGCTCTTTATGGTTGGCGTGCTCGGCACCg                                                               >  1:3107077/1‑93 (MQ=255)

                             tGATCAGGCCACCATGCGCGTCGCCGATATGTTTATGACCTTCCCGACCTCGATTCTGTCGCTCTTTATGGTTGGCGTGCTCGGCACCGGGCt                                                           <  1:2036058/93‑1 (MQ=255)

                             tGATCAGGCCACCATGCGCGTCGCCGATATGTTTATGACCTTCCCGACCTCGATTCTGTCGCTCTTTATGGTTGGCGTGCTCGGCACCGGGCt                                                           <  1:3169605/93‑1 (MQ=255)

                                  aagCCACCATGCGCGTCGCCGATATGTTTATGACCTTCCCGACCTCGATTCTGTCGCTCTTTATGGTTGGCGTGCTCGGCACCGGGCTGACCa                                                      <  2:980334/91‑1 (MQ=255)

                                  aGGCCACCATGCGCGTCGCCGATATGTTTATGACCTTCCCGACCTCGATTCTGTCGCTCTTTATGGTTGGCGTGCTCGGCACCGGGCTGACCa                                                      >  1:222015/1‑93 (MQ=255)

                                    gCCACCATGCGCGTCGCCGATATGTTTATGACCTTCCCGGCCTCGATTCTGTCGCTCTTTATGGTTGGCGTGCTCGGCACCGGGCTGACCAAc                                                    <  1:1650689/93‑1 (MQ=255)

                                      caccaTGCGCGTCGCCGATATGTTTATGACCTTCCCGACCTCGATTCTGTCGCTCTTTATGGTTGGCGTGCTCGGCACCGGGCTGACCAACGt                                                  >  2:359050/1‑93 (MQ=255)

                                       accaTGCGCGTCGCCGATATGTTTATGACCTTCCCGACCTCGATTCTGTCGCTCTTTATGGTTGGCGTGCTCGGCACCGGGCTGACCAACGTa                                                 >  2:4168955/1‑93 (MQ=255)

                                       accaTGCGCGTCGCCGATATGTTTATGACCTTCCCGACCTCGATTCTGTCGCTCTTTATGGTTGGCGTGCTCGGCACCGGGCTGACCAACGTa                                                 >  1:4009448/1‑93 (MQ=255)

                                       accaTGCGCGTCGCCGATATGTTTATGACCTTCCCGACCTCGATTCTGTCGCTCTTTATGGTTGGCGTGCTCGGCACCGGGCTGACCAACGTa                                                 >  1:3059746/1‑93 (MQ=255)

                                        ccaTGCGCGTCGCCGATATGTTTATGATCGCCCCGACCTCGATTCTGTCGCTCTTTATGGTTGGCGTGCTCGGCACCGGGCTGACCAACGTaa                                                >  2:1522815/1‑93 (MQ=255)

                                        ccaTGCGCGTCGCCGATATGTTTATGACCTTCCCGACCTCGATTCTGTCGCTCTTTATGGTTGGCGTGCTCGGCACCGGGCTGACCAACGTaa                                                >  1:2385968/1‑93 (MQ=255)

                                        ccaTGCGCGTCGCCGATATGTTTATGACCTTCCCGACCTCGATTCTGTCGCTCTTTATGGTTGGCGTGCTCGGCACCGGGCTGACCAACGTaa                                                >  2:1729558/1‑93 (MQ=255)

                                          aTGCGCGTCGCCGATATGTTTATGACCTTCCCGACCTCGATTCTGTCGTTCTTTATGGTTGGCGTGCTCGGCACCGGGCTGACCAACGTAAtt                                              >  2:3136093/1‑93 (MQ=255)

                                          aTGCGCGTCGCCGATATGTTTATGACCTTCCCGACCTCGATTCTGTCGCTCTTTATGGTTGGCGTGCTCGGCACCGGGCTGACCAACGTAAtt                                              >  1:1935021/1‑93 (MQ=255)

                                           tGCGCGTCGCTGATATGTTTATGACCTTCCCGACCTCGATTCTGTCGCTCTTTATGGTTGGCGTGCTCGGCACCGGGCTGACCAACGTAATTa                                             <  2:2753912/93‑1 (MQ=255)

                                           tGCGCGTCGCCGATATGTTTATGACCTTCCCGACCTCGATTCTGTCGCTCTTTATGGTTGGCGTGCTCGGCACCGGGCTGACCAACGTAATTa                                             <  1:3417251/93‑1 (MQ=255)

                                             cgcgTCGCCGATATGTTTATGACCTTCCCGACCTCGATTCTGTCGCTCTTTATGGTTAGCGTGCTCGGCACCGGGCTGACCAACGTAATTatc                                           <  2:4676028/93‑1 (MQ=255)

                                               cgTCGCCGATATGTTTATGACCTTCCCGACCTCGATTCTGTCGCTCTTTATGGTTGGCGTGCTCGGCACCGGGCTGACCAACGTAATTatcgc                                         <  1:649839/93‑1 (MQ=255)

                                                gTCGCCGATATGTTTATGACCTTCCCGACCTCGATTCTGTCGCTCTTTATGGTTGGCGTGCTCGGCACCGGGCTGACCAACGTAATTatcgcc                                        >  1:559111/1‑93 (MQ=255)

                                                gTCGCCGATATGTTTATGACCTTCCCGACCTCGATTCTGTCGCTCTTTATGGTTGGCGTGCTCGGCACCGGGCTGACCAACGTAATTatcgcc                                        >  1:2180750/1‑93 (MQ=255)

                                                 tCGCCGATATGTTTATGACCTTCCCGACCTCGATTCTGTCGCTCTTTATGGTTGGCGTGCTCGGCACCGGGCTGACCAACGTAATTatcgcca                                       >  1:2857687/1‑93 (MQ=255)

                                                 tCGCCGATATGTTTATGACCTTCCCGACCTCGATTCTGTCGCTCTTTATGGTTGGCGTGCTCGGCACCGGGCTGACCAACGTAATTatcgcca                                       >  1:3253066/1‑93 (MQ=255)

                                                  cGCCGATATGTTTATGACCTTCCCGACCTCGATTCTGTCGCTCTTTATGGTTGGCGTGCTCGGCACCGGGCTGACCAACGTAATTatcgccat                                      >  2:4386629/1‑93 (MQ=255)

                                                   gcagATATGTTTATGACCTTCCCGACCTCGATTCTGTCCCTCTTTATGGTTGGCGTGCTCGGCACCGGGCTGACCAACGTAATTatcgccatc                                     <  2:3717696/90‑1 (MQ=255)

                                                   gCCGATATGTTTATGACCTTCCCGACCTCGATTCTGTCGCTCTTTATGGTTGGCGTGCTCGGCACCGGGCTGACCAACGTAATTatcgccatc                                     >  2:2526653/1‑93 (MQ=255)

                                                    ccGATATGTTTATGACCTTCCCGACCTCGATTCTGTCGCTCTTTATGGTTGGCGTGCTCGGCACCGGGCTGACCAACGTAATTatcgccatcg                                    >  1:495812/1‑93 (MQ=255)

                                                      gATATGTTTATGACCTTCCCGACCTCGATTCTGTCGCTCTTTATGGTTGGCGTGCTCGGCACCGGGCTGACCAACGTAATTatcgccatcgcc                                  >  1:1608151/1‑93 (MQ=255)

                                                      gATATGTTTATGACCTTCCCGACCTCGATTCTGTCGCTCTTTATGGTTGGCGTGCTCGGCACCGGGCTGACCAACGTAATTatcgccatcgcc                                  >  1:994909/1‑93 (MQ=255)

                                                      gATATGTTTATGACCTTCCCGACCTCGATTCTGTCGCTCTTTATGGTTGGCGTGCTCGGCACCGGGCTGACCAACGTAATTatcgccatcgcc                                  >  1:4616022/1‑93 (MQ=255)

                                                       atatGTTTATGACCTTCCCGACCTCGATTCTGTCGCTCTTTATGGTTGGCGTGCTCGGCACCGGGCTGACCAACGTAATTATCGCCATCGccc                                 >  1:1780831/1‑93 (MQ=255)

                                                        tatGTTTATGACCTTCCCGACCTCGATTCTGTCGTTCTTTATGGTTGGCGTGCTCGGCACCGGGCTGACCAACGTAATTATCGCCATCGCCCt                                >  2:4017799/1‑93 (MQ=255)

                                                        tatGTTTATGACCTTCCCGACCTCGATTCTGTCGCTCTTTATGGTTGGCGTGCTCGGCACCGGGCTGACCAACGTAATTATCGCCATCGCCCt                                >  1:4282/1‑93 (MQ=255)

                                                        tatGTTTATGACCTTCCCGACCTCGATTCTGTCGCTCTTTATGGTTGGCGTGCTCGGCACCGGGCTGACCAACGTAATTATCGCCATCGCCCt                                >  2:4708277/1‑93 (MQ=255)

                                                        tatGTTTATGACCTTCCCGACCTCGATTCTGTCGCTCTTTATGGTTGGCGTGCTCGGCACCGGGCTGACCAACGTAATTATCGCCATCGCCCt                                >  2:2698932/1‑93 (MQ=255)

                                                        tatGTTTATGACCTTCCCGACCTCGATTCTGTCGCTCTTTATGGTTGGCGTGCTCGGCACCGGGCTGACCAACGTAATTATCGCCATCGCCCt                                <  1:1347591/93‑1 (MQ=255)

                                                         atGTTTATGACCTTCCCGACCTCGATTCTGTCGCTCTTTATGGTTAGCGTGCTCGGCACCGGGCTGACCAACGTAATTATCGCCATCGCCCTg                               >  1:398580/1‑93 (MQ=255)

                                                          tGTTTATGACCTTCCCGACCTCGATTCTGTCGCTCTTTATGGTTGGCGTGCTCGGCACCGGGCTGACCAACGTAATTATCGCCATCGCCCTGt                              <  2:1428167/93‑1 (MQ=255)

                                                           ttttATGACCTTCCCGACCTCGATTCTGTCGCTCTTTATGGTTGGCGTGCTCGGCCCCGGGCTGACCAACGTAATTATCGCCATCGCCCTGTc                             <  2:3627903/92‑1 (MQ=255)

                                                           gTTTATGACCTTCCCGACCTCGATTCTGTCGCTCTTTATGGTTGGCGTGCTCGGCACCGGGCTGACCAACGTAATTATCGCCATCGCCCTGTc                             >  1:1000290/1‑93 (MQ=255)

                                                              tATGACCTTCCCGACCTCGATTCTGTCGCTCTTTATGGTTGGCGTGCTCGGCACCGGGCTGACCAACGTAATTATCGCCATCGCCCTGTCGCa                          >  1:2425259/1‑93 (MQ=255)

                                                              tATGACCTTCCCGACCTCGATTCTGTCGCTCTTTATGGTTGGCGTGCTCGGCACCGGGCTGACCAACGTAATTATCGCCATCGCCCTGTCGCa                          >  1:636125/1‑93 (MQ=255)

                                                               aTGACCTTCCCGACCTCGATTCTGTCGCTCTTTATGGTTGGCGTGCTCGGCACCCGGCTGACCAACGTAATTATCGCCATCGCCCTGTCGCAc                         >  1:1337923/1‑93 (MQ=255)

                                                                  gccTTCCCGACCTCGATTCTGTCGCTCTTTATGGTTGGCGTGCTCGGCACCGGGCTGACCAACGTAATTATCGCCATCGCCCTGTCGCACTgg                      <  2:3722990/92‑1 (MQ=255)

                                                                  aCCTTCCCGACCTCGATTCTGTCGCTCTTTATGGTTGGCGTGCTCGGCACCGGGCTGACCAACGTAATTATCGCCATCGCCCTGTCGCACTgg                      >  1:3642635/1‑93 (MQ=255)

                                                                  aCCTTCCCGACCTCGATTCTGTCGCTCTTTATGGTTGGCGTGCTCGGCACCGGGCTGACCAACGTAATTATCGCCATCGCCCTGTCGCACTgg                      <  2:2854030/93‑1 (MQ=255)

                                                                  aCCTTCCCGACCTCGATTCTGTCGCTCTTTATGGTTGGCGTGCTCGGCACCGGGCTGACCAACGTAATTATCGCCATCGCCCTGTCGCACTgg                      <  2:691216/93‑1 (MQ=255)

                                                                    cTTCCCGACCTCGATTCTGTCGCTCTTTATGGTTGGCGTGCTCGGCACCGGGCTGACCAACGTAATTATCGCCATCGCCCTGTCGCACTGGGc                    >  1:3660088/1‑93 (MQ=255)

                                                                    cTTCCCGACCTCGATTCTGTCGCTCTTTATGGTTGGCGTGCTCGGCACCGGGCTGACCAACGTAATTATCGCCATCGCCCTGTCGCACTGGGc                    >  1:4285188/1‑93 (MQ=255)

                                                                    cTTCCCGACCTCGATTCTGTCGCTCTTTATGGTTGGCGTGCTCGGCACCGGGCTGACCAACGTAATTATCGCCATCGCCCTGTCGCACTGGGc                    >  1:2399146/1‑93 (MQ=255)

                                                                     ttCCCGACCTCGATTCTGTCGCTCTTTATGGTTGGCGTGCTCGGCACCGGGCTGACCAACGTAATTATCGCCATCGCCCTGTCGCACTGGGCg                   >  2:2126067/1‑93 (MQ=255)

                                                                     ttCCCGACCTCGATTCTGTCGCTCTTTATGGTTGGCGTGCTCGGCACCGGGCTGACCAACGTAATTATCGCCATCGCCCTGTCGCACTGGGCg                   >  2:3488187/1‑93 (MQ=255)

                                                                      tCCCGACCTCGATTCTGTCGCTCTTTATGGTTGGCGTGCTCGGCACCGGGCTGACCAACGTAATTATCGCCATCGCCCTGTCGCACTGGGCGt                  <  1:4691607/93‑1 (MQ=255)

                                                                       cgcGACCTCGATTCTGTCGCTCTTTATGGTTGGCGTGCTCGGCACCGGGCTGACCAACGTAATTATCGCCATCGCCCTGTCGCACTGGGCGTg                 <  2:3285949/91‑1 (MQ=255)

                                                                       cccGACCTCGATTCTGTCGCTCTTTATGGTTGGCGTGCTCGGCACCGGGCTGACCAACGTAATTATCGCCATCGCCCTGTCGCACTGGGCGTg                 <  2:3285948/93‑1 (MQ=255)

                                                                       cccGACCTCGATTCTGTCGCTCTTTATGGTTGGCGTGCTCGGCACCGGGCTGACCAACGTAATTATCGCCATCGCCCTGTCGCACTGGGCGTg                 <  1:291035/93‑1 (MQ=255)

                                                                        ccGACCTCGATTCTGTCGCTCTTTATGGTTGGCGTGCTCGGCACCGGGCTGACCAACGTAATTATCGCCATCGCCCTGTCGCACTGGGCGTgg                >  1:3786116/1‑93 (MQ=255)

                                                                        ccGACCTCGATTCTGTCGCTCTTTATGGTTGGCGTGCTCGGCACCGGGCTGACCAACGTAATTATCGCCATCGCCCTGTCGCACTGGGCGTgg                >  1:1063886/1‑93 (MQ=255)

                                                                        ccGACCTCGATTCTGTCGCTCTTTATGGTTGGCGTGCTCGGCACCGGGCTGACCAACGTAATTATCGCCATCGCCCTGTCGCACTGGGCGTgg                <  1:2755517/93‑1 (MQ=255)

                                                                          gACCTCGATTCTGTCGCTCTTTATGGTTGGCGTGCTCGGCACCGGGCTGACCAACGTAATTATCGCCATCGCCCTGTCGCACTGGGCGTGGTa              <  2:3092967/93‑1 (MQ=255)

                                                                          gACCTCGATTCTGTCGCTCTTTATGGTTGGCGTGCTCGGCACCGGGCTGACCAACGTAATTATCGCCATCGCCCTGTCGCACTGGGCGTGGTa              <  2:160945/93‑1 (MQ=255)

                                                                           aCCTCGATTCTGTCGCTCTTTATGGTTGGCGTGCTCGGCACCGGGCTGACCAACGTAATTATCGCCATCGCCCTGTCGCACTGGGCGTGGTAt             <  2:2850890/93‑1 (MQ=255)

                                                                           aCCTCGATTCTGTCGCTCTTTATGGTTGGCGTGCTCGGCACCGGGCTGACCAACGTAATTATCGCCATCGCCCTGTCGCACTGGGCGTGGTAt             >  2:543747/1‑93 (MQ=255)

                                                                            ccTCGATTCTGTCGCTCTTTATGGTTGGCGTGCTCGGCACCGGGCTGACCAACGTAATTATCGCCATCGCCCTGTCGCACTGGGCGTGGTCTg            >  2:2418100/1‑93 (MQ=255)

                                                                              tCGATTCTGTCGCTCTTTATGGTTGGCGTGCTCGGCACCGGGCTGACCAACGTAATTATCGCCATCGCCCTGTCGCACTGGGCGTGGTATGCa          >  2:2841172/1‑93 (MQ=255)

                                                                              tCGATTCTGTCGCTCTTTATGGTTGGCGTGCTCGGCACCGGGCTGACCAACGTAATTATCGCCATCGCCCTGTCGCACTGGGCGTGGTATGCa          >  1:584461/1‑93 (MQ=255)

                                                                               cGATTCTGTCGCTCTTTATGGTTGGCGTGCTCGGCACCGGGCTGACCAACGTAATTATCGCCATCGCCCTGTCGCACTGGGCGTGGTATGCAc         >  1:3268304/1‑93 (MQ=255)

                                                                               cGATTCTGTCGCTCTTTATGGTGGGCGTCCTCGGCACCGGGCTGACAAACGTAATTATCGCCATCGCCCTGTCGCACTGGGCGTGGTATGCAc         <  1:664796/93‑1 (MQ=255)

                                                                                 aTTCTGTCGCTCTTTATGGTTGGCGTGCTCGGCACCGGGCTGACCAACGTAATTATCGCCATCGCCCTGTCGCACTGGGCGTGGTATGCACGc       >  2:4343441/1‑93 (MQ=255)

                                                                                  ttCTGTCGCTCTTTATGGTTGGCGTGCTCGGCACCGGGCTGACCAACGTAATTATCGCCATCGCCCTGTCGCACTGGGCGTGGTATGCACGCa      >  2:4463748/1‑93 (MQ=255)

                                                                                  ttCTGTCGCTCTTTATGGTTGGCGTGCTCGGCACCGGGCTGACCAACGTAATTATCGCCATCGCCCTGTCGCACTGGGCGTGGTATGCACGCa      >  1:3023792/1‑93 (MQ=255)

                                                                                  ttCTGTCGCTCTTTATGGTTGGCGTGCTCGGCACCGGGCTGACCAACGTAATTATCGCCATCGCCCTGTCGCACTGGGCGTGGTATGCACGCa      <  1:3510055/93‑1 (MQ=255)

                                                                                      gTCGCTCTTTATGGTTGGCGTGCTCGGCACCGGGCTGACCAACGTAATTATCGCCATCGCCCTGTCGCACTGGGCGTGGTATGCACGCATGGt  <  1:1110620/93‑1 (MQ=255)

GGCAGCGCCGGGTTGATTGGCGGGCGCGTTGATCAGGCCACCATGCGCGTCGCCGATATGTTTATGACCTTCCCGACCTCGATTCTGTCGTTCTTTATGGTTGGCGTGCTCGGCACCGGGCTGACCAACGTAATTATCGCCATCGCCCTGTCGCACTGGGCGTGGTATGCACGCATGGT  >  NC_000913/3614092‑3614270

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 17 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`