BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,628,759	G→A	R488C (CGT→TGT)	yhiJ ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,628,759	0	G	A	98.1%	1114.3 / 1.2	315	R488C (CGT→TGT)	yhiJ	hypothetical protein
Reads supporting (aligned to +/- strand): ref base G (2/4); new base A (158/151); total (160/155)
Fisher's exact test for biased strand distribution p-value = 4.42e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.98e-01

Only 100 of 325 total aligned reads displayed.

ACTTCCAGGGAGTGGTTATCTCAGGCCCTGCACCAGGGTTAATTTGGCAATTATGCATGCTGAACATTCTTCCAGCATGTTTACTGTCACGGGCGTTAAGACCTAAAACTGTCCCCGTTTCGCCTATGCGTTCTCTTCCACCATTTCCGTTATGTGCAACAACTTGATTTATACCTAACA  >  NC_000913/3628669‑3628848

aCTTCCAGGGAGTGGTTATCTCAGGCCCTGCACCAGGGTTAATTTGGCAATTATGCATGCTGAACATTCTTCCAGCATGTTTACTGTCACagg                                                                                         >  1:4295128/1‑93 (MQ=255)

 cTTCCAGGGAGTGGTTATCTCAGGCCCTGCACCAGGGTTAATTTGGCAATTATGCATGCTGAACATTCTTCCAGCATGTTTACTGTCACAGGc                                                                                        <  1:3429761/93‑1 (MQ=255)

 cTTCCAGGGAGTGGTTATCTCAGGCCCTGCACCAGGGTTAATTTGGCAATTATGCATGCTGAACATTCTTCCAGCATGTTTACTGTCACAGGc                                                                                        <  2:825995/93‑1 (MQ=255)

 cTTCCAGGGAGTGGTTATCTCAGGCCCTGCACCAGGGTTAATTTGGCAATTATGCATGCTGAACATTCTTCCAGCATGGTTACTGTCACAGGc                                                                                        <  2:4099500/93‑1 (MQ=255)

   tCCAGGGAGTGGTTATCTCAGGCCCTGCACCAGGGTTAATTTGGCAATTATGCATGCTGCACATTCTTCCAGCATGTTTACTGTCACAGGCGt                                                                                      >  1:4566271/1‑93 (MQ=255)

    ccAGGGAGTGGTTATCTCAGGCCCTGCACCAGGGTTAATTTGGCAATTATGCATGCTGAACATTCTTCCAGCATGTTTACTGTCACAGGCGtt                                                                                     >  2:88753/1‑93 (MQ=255)

     cAGGGAGTGGTTATCTCAGGCCCTGCACCAGGGTTAATTTGGCAATTATGCATGCTGAACATTCTTCCAGCATGTTTACTGTCACAGGCGTTa                                                                                    >  2:4462886/1‑93 (MQ=255)

      aGGGAGTGGTTATCTCAGGCCCTGCACCAGGGTTAATTTGGCAATTATGCATGCTGAACATTCTTCCAGCATGTTTACTGTCACAGGCGTTaa                                                                                   <  2:372084/93‑1 (MQ=255)

       gggAGTGGTTATCTCAGGCCCTGCACCAGGGTTAATTTGGCAATTATGCATGCTGAACATTCTTCCAGCATGTTTACTGTCACGGGCGTTAAg                                                                                  <  1:3128377/93‑1 (MQ=255)

       gggAGTGGTTATCTCAGGCCCTGCACCAGGGTTAATTTGGCAATTATGCATGCTGAACATTCTTCCAGCATGTTTACTGTCACAGGCGTTAAg                                                                                  >  2:3384830/1‑93 (MQ=255)

       gggAGTGGTTATCTCAGGCCCTGCACCAGGGTTAATTTGGCAATTATGCATGCTGAACATTCTTCCAGCATGTTTACTGTCACAGGCGTTAAg                                                                                  >  2:852102/1‑93 (MQ=255)

         gAGTGGTTATCTCAGGCCCTGCACCAGGGTTAATTTGGCAATTATGCATGCTGAACATTCTTCCAGCATGTTTACTGTCACAGGCGTTAAGAc                                                                                <  1:1357985/93‑1 (MQ=255)

           gTGGTTATCTCAGGCCCTGCACCAGGGTTAATTTGGCAATTATGCATGCTGAACATTCTTCCAGCATGTTTACTGTCACAGGCGTTAAGACCt                                                                              >  1:1880342/1‑93 (MQ=255)

           gTGGTTATCTCAGGCCCTGCACCAGGGTTAATTTGGCAATTATGCATGCTGAACATTCTTCCAGCATGTTTACTGTCACAGGCGTTAAGACCt                                                                              <  2:1820169/93‑1 (MQ=255)

           gTGGTTATCTCAGGCCCTGCACCAGGGTTAATTTGGCAATTATGCATGCTGAACATTCTTCCAGCATGTTTACTGTCACAGGCGTTAAGACCt                                                                              <  2:1519232/93‑1 (MQ=255)

           gTGGTTATCTCAGGCCCTGCACCAGGGTTAATTTGGCAATTATGCATGCTGAACATTCTTCCAGCATGTTTACTGTCACAGGCGTTAAGACCt                                                                              >  1:1382103/1‑93 (MQ=255)

           gTGGTTATCTCAGGCCCTGCACCAGGGTTAATTTGGCAATTATGCATGCTGAACATTCTTCCAGCATGTTTACTGTCACAGGCGTTAAGACCt                                                                              >  1:388448/1‑93 (MQ=255)

            tGGTTATCTCAGGCCCTGCACCAGGGTTAATTTGGCAATTATGCATGCTGAACATTCTTCCAGCATGTTTACTGTCACAGGCGTTAAGACCTa                                                                             <  1:692762/93‑1 (MQ=255)

               ttATCTCAGGCCCTGCACCAGGGTTAATTTGGCAATTATGCATGCTGAACATTCTTCCAGCATGTTTACTGTCACAGGCGTTAAGACCTaaaa                                                                          <  2:1268843/93‑1 (MQ=255)

                tATCTCAGGCCCTGCACCAGGGTTAATTTGGCAATTATGCATGCTGAACATTCTTCCAGCATGTTTACTGTCACAGGCGTTAAGACCTAAAAc                                                                         >  2:545444/1‑93 (MQ=255)

                 aTCTCAGGCCCTGCACCAGGGTTAATTTGGCAATTATGCATGCTGAACATTCTTCCAGCATGTTTCCTGTCACAGGCGTTAAGACCTAAAACt                                                                        <  2:1603420/93‑1 (MQ=255)

                    tcAGGCCCTGCACCAGGGTTAATTTGGCAATTATGCATGCTGAACATTCTTCCAGCATGTTTACTGTCACAGGCGTTAAGACCTAAAACTGTc                                                                     <  2:2393821/93‑1 (MQ=255)

                     cAGGCCCTGCACCAGGGTTAATTTGGCAATTATGCATGCTGAACATTCTTCCAGCATGTTTACTGTCACAGGCGTTAAGACCTAAAACTGTcc                                                                    <  2:4064975/93‑1 (MQ=255)

                     cAGGCCCTGCACCAGGGTTAATTTGGCAATTATGCATGCTGAACATTCTTCCAGCATGTTTACTGTCACAGGCGTTAAGACCTAAAACTGTcc                                                                    >  1:1133247/1‑93 (MQ=255)

                     cAGGCCCTGCACCAGGGTTAATTTGGCAATTATGCATGCTGAACATTCTTCCAGCATGTTTACTGTCACAGGCGTTAAGACCTAAAACTGTcc                                                                    <  1:2232763/93‑1 (MQ=255)

                      aGGCCCTGCACCAGGGTTAATTTGGCAATTATGCATGCTGAACATTCTTCCAGCATGTTTACTGTCACAGGCGTTAAGACCTAAAACTGTccc                                                                   >  1:552552/1‑93 (MQ=255)

                      aGGCCCTGCACCAGGGTTAATTTGGCAATTATGCATGCTGAACATTCTTCCAGCATGTTTACTGTCACAGGCGTTAAGACCTAAAACTGTccc                                                                   >  2:1222981/1‑93 (MQ=255)

                      aGGCCCTGCACCAGGGTTAATTTGGCAATTATGCATGCTGAACATTCTTCCAGCATGTTTACTGTCACAGGCGTTAAGACCTAAAACTGTccc                                                                   >  1:2858539/1‑93 (MQ=255)

                      aGGCCCTGCACCAGGGTTAATTTGGCAATTATGCATGCTGAACATTCTTCCAGCATGTTTACTGTCACAGGCGTTAAGACCTAAAACTGTccc                                                                   <  2:3775387/93‑1 (MQ=255)

                      aGGCCCTGCACCAGGGTTAATTTGGCAATTATGCATGCTGAACATTCTTCCAGCATGTTTACTGTCACAGGCGTTAAGACCTAAAACTGTccc                                                                   <  2:3610937/93‑1 (MQ=255)

                         cccTGCACCAGGGTTAATTTGGCAATTATGCATGCTGAACATTCTTCCAGCATGTTTACTGTCACAGGCGTTAAGACCTAAAACTGTCCCCGt                                                                >  2:2986421/1‑93 (MQ=255)

                          ccTGCACCAGGGTTAATTTGGCAATTATGCATGCTGAACATTCTTCCAGCATGTTTACTGTCACAGGCGTTAAGACCTAAAACTGTCCCCGtt                                                               <  2:2326244/93‑1 (MQ=255)

                          ccTGCACCAGGGTTAATTTGGCAATTATGCATGCTGAACATTCTTCCAGCATGTTTACTGTCACAGGCGTTAAGACCTAAAACTGTCCCCGtt                                                               >  1:4512394/1‑93 (MQ=255)

                          ccTGCACCAGGGTTAATTTGGCAATTATGCATGCTGAACATTCTTCCAGCATGTTTACTGTCACAGGCGTTAAGACCTAAAACTGTCCCCGtt                                                               <  1:3024787/93‑1 (MQ=255)

                          ccTGCACCAGGGTTAATTTGGCAATTATGCATGCTGAACATTCTTCCAGCATGTTTACTGTCACAGGCGTTAAGACCTAAAACTGTCCCCGtt                                                               >  1:3180861/1‑93 (MQ=255)

                            tGCACCAGGGTTAATTTGGCAATTATGCATGCTGAACATTCTTCCAGCATGTTTACTGTCACAGGCGTTAAGACCTAAAACTGTCCCCGTTTc                                                             >  1:4380845/1‑93 (MQ=255)

                              cACCAGGGTTAATTTGGCAATTATGCATGCTGAACATTCTTCCAGCATGTTTACTGTCACAGGCGTTAAGACCTAAAACTGTCCCCGTTTCGc                                                           >  2:2894788/1‑93 (MQ=255)

                               aCCAGGGTTAATTTGGCAATTATGCATGCTGAACATTCTTCCAGCATGTTTACTGTCACAGGCGTTAAGACCTAAAACTGTCCCCGTTTCGcc                                                          <  1:1433978/93‑1 (MQ=255)

                                 cAGGGTTAATTTGGCAATTATGCATGCTGAACATTCTTCCAGCATGTTTACTGTCACAGGCGTTAAGACCTAAAACTGTCCCCGTTTCGCCTa                                                        <  1:132252/93‑1 (MQ=255)

                                 cAGGGTTAATTTGGCAATTATGCATGCTGAACATTCTTCCAGCATGTTTACTGTCACAGGCGTTAAGACCTAAAACTGTCCCCGTTTCGCCTa                                                        >  2:2004639/1‑93 (MQ=255)

                                  aGGGTTAATTTGGCAATTATGCATGCTGAACATTCTTCCAGCATGTTTACTGTCACAGGCGTTAAGACCTAAAACTGTCCCCGTTTCGCCTAt                                                       <  1:3584240/93‑1 (MQ=255)

                                   gggTTAATTTGGCAATTATGCATGCTGAACATTCTTCCAGCATGTTTACTGTCACAGGCGTTAAGACCTAAAACTGTCCCCGTTTCGCCTATg                                                      <  2:2845294/93‑1 (MQ=255)

                                     gTTAATTTGGCAATTATGCATGCTGAACATTCTTCCAGCATGTTTACTGTCACAGGCGTTAAGACCTAAAACTGTCCCCGTTTCGCCTATGCg                                                    >  1:2599774/1‑93 (MQ=255)

                                         aTTTGGCAATTATGCATGCTGAACATTCTTCCAGCATGTTTACTGTCACAGGCGTTAAGACCTAAAACTGTCCCCGTTTCGCCTATGCGTtct                                                >  2:1301744/1‑93 (MQ=255)

                                          tttGGCAATTATGCATGCTGAACATTCTTCCAGCATGTTTACTGTCACAGGCGTTAAGACCTAAAACTGTCCCCGTTTCGCCTATGCGTtctc                                               >  2:2300435/1‑93 (MQ=255)

                                            tGGCAATTATGCATGCTGAACATTCTTCCAGCATGTTTACTGTCACAGGCGTTAAGACCTAAAACTGTCCCCGTTTCGCCTATGCGTTCTCtt                                             >  1:1167907/1‑93 (MQ=255)

                                             ggCAATTATGCATGCTGAACATTCTTCCAGCATGTTTACTGTCACAGGCGTTAAGACCTAAAACTGTCCCCGTTTCGCCTATGCGTTCTCTTc                                            <  1:1077927/93‑1 (MQ=255)

                                             ggCAATTATGCATGCTGAACATTCTTCCAGCATGTTTACTGTCACAGGCGTTAAGACCTAAAACTGTCCCCGTTTCGCCTATGCGTTCTCTTc                                            >  1:817258/1‑93 (MQ=255)

                                             ggCAATTATGCATGCTGAACATTCTTCCAGCATGTTTACTGTCACAGGCGTTAAGACCTAAAACTGTCCCCGTTTCGCCTATGCGTTCTCTTc                                            >  1:4029622/1‑93 (MQ=255)

                                              gCAATTATGCATGCTGAACATTCTTCCAGCATGTTTACTGTCACAGGCGTTAAGACCTAAAACTGTCCCCGTTTCGCCTATGCGTTCTCTTcc                                           <  1:4251759/93‑1 (MQ=255)

                                              gCAATTATGCATGCTGAACATTCTTCCAGCATGTTTACTGTCACAGGCGTTAAGACCTAAAACTGTCCCCGTTTCGCCTATGCGTTCTCTTcc                                           >  1:3019818/1‑93 (MQ=255)

                                               cAATTATGCATGCTGAACATTCTTCCAGCATGTTTACTGTCACAGGCGTTAAGACCTAAAACTGTCCCCGTTTCGCCTATGCGTTCTCTTcca                                          >  1:746806/1‑93 (MQ=255)

                                                aaTTATGCATGCTGAACATTCTTCCAGCATGTTTACTGTCACAGGCGTTAAGACCTAAAACTGTCCCCGTTTCGCCTATGCGTTCTCTTccac                                         >  2:4318928/1‑93 (MQ=255)

                                                 aTTATGCATGCTGAACATTCTTCCAGCATGTTTACTGTCACAGGCGTTAAGACCTAAAACTGTCCCCGTTTCGCCTATGCGTTCTCTTccacc                                        >  2:2783984/1‑93 (MQ=255)

                                                 aTTATGCATGCTGAACATTCTTCCAGCATGTTTACTGTCACAGGCGTTAAGACCTAAAACTGTCCCCGTTTCGCCTATGCGTTCTCTTccacc                                        <  2:3142385/93‑1 (MQ=255)

                                                 aTTATGCATGCTGAACATTCTTCCAGCATGTTTACTGTCACAGGCGTTAAGACCTAAAACTGTCCCCGTTTCGCCTATGCGTTCTCTTccacc                                        >  1:3845587/1‑93 (MQ=255)

                                                  ttATGCATGCTGAACATTCTTCCAGCATGTTTACTGTCACAGGCGTTAAGACCTAAAACTGTCCCCGTTTCGCCTATGCGTTCTCTTccacca                                       >  2:1934592/1‑93 (MQ=255)

                                                  ttATGCATGCTGAACATTCTTCCAGCATGTTTACTGTCACAGGCGTTAAGACCTAAAACTGTCCCCGTTTCGCCTATGCGTTCTCTTccacca                                       <  2:66484/93‑1 (MQ=255)

                                                  ttATGCATGCTGAACATTCTTCCAGCATGTTTACTGTCACAGGCGTTAAGACCTAAAACTGTCCCCGTTTCGCCTATGCGTTCTCTTccacca                                       >  1:4190734/1‑93 (MQ=255)

                                                      gcatgcTGAACATTCTTCCAGCATGTTTACTGTCACAGGCGTTAAGACCTAAAACTGTCCCCGTTTCGCCTATGCGTTCTCTTCCACCATTTc                                   <  2:3819680/93‑1 (MQ=255)

                                                      gcatgcTGAACATTCTTCCAGCATGTTTACTGTCACAGGCGTTAAGACCTAAAACTGTCCCCGTTTCGCCTATGCGTTCTCTTCCACCATTTc                                   <  1:951568/93‑1 (MQ=255)

                                                      gcatgcTGAACATTCTTCCAGCATGTTTACTGTCACAGGCGTTAAGACCTAAAACTGTCCCCGTTTCGCCTATGCGTTCTCTTCCACCATTTc                                   <  1:501103/93‑1 (MQ=255)

                                                        atgcTGAACATTCTTCCAGCATGTTTACTGTCACAGGCGTTAAGACCTAAAACTGTCCCCGTTTCGCCTATGCGTTCTCTTCCACCATTTCCg                                 >  2:3235304/1‑93 (MQ=255)

                                                         tgcTGAACATTCTTCCAGCATGTTTACTGTCACGGGCGTTAAGACCTAAAACTGTCCCCGTTTCGCCTATGCGTTCTCTTCCACCATTTCCGt                                >  2:4530789/1‑93 (MQ=255)

                                                         tgcTGAACATTCTTCCAGCATGTTTACTGTCACAGGCGTTAAGACCTAAAACTGTCCCCGTTTCGCCTATGCGTTCTCTTCCACCATTTCCGt                                <  2:4651086/93‑1 (MQ=255)

                                                           cTGAACATTCTTCCAGCATGTTTACTGTCACAGGCGTTAAGACCTAAAACTGTCCCCGTTTCGCCTATGCGTTCTCTTCCACCATTTCCGTTa                              <  2:2114091/93‑1 (MQ=255)

                                                              aaCATTCTTCCAGCATGTTTACTGTCACAGGCGTTAAGACCTAAAACTGTCCCCGTTTCGCCTATGCGTTCTCTTCCACCATTTCCGTTAtgt                           <  1:2193976/93‑1 (MQ=255)

                                                               aCATTCTTCCAGCATGTTTACTGTCACAGGCGTTAAGACCTAAAACTGTCCCCGTTTCGCCTATGCGTTCTCTTCCACCATTTCCGTTAtgtg                          >  2:143891/1‑93 (MQ=255)

                                                                 aTTCTTCCAGCATGTTTACTGTCACAGGCGTTAAGACCTAAAACTGTCCCCGTTTCGCCTATGCGTTCTCTTCCACCATTTCCGTTATGTGca                        <  2:3066075/93‑1 (MQ=255)

                                                                 aTTCTTCCAGCATGTTTACTGTCACAGGCGTTAAGACCTAAAACTGTCCCCGTTTCGCCTATGCGTTCTCTTCCACCATTTCCGTTATGTGca                        >  2:2654305/1‑93 (MQ=255)

                                                                  ttcttcCAGCATGTTTACTGTCACAGGCGTTAAGACCTAAAACTGTCCCCGTTTCGCCCATGCGTTCTCTTCCACCATTTCCGTTATGTGcaa                       <  1:4593589/93‑1 (MQ=255)

                                                                     ttcCAGCATGTTTACTGTCACAGGCGTTAAGACCTAAAACTGTCCCCGTTTCGCCTATGCGTTCTCTTCCACCATTTCCGTTATGTGcaacaa                    <  2:1366732/93‑1 (MQ=255)

                                                                     ttcCAGCATGTTTACTGTCACAGGCGTTAAGACCTAAAACTGTCCCCGTTTCGCCTATGCGTTCTCTTCCACCATTTCCGTTATGTGcaacaa                    >  1:2669498/1‑93 (MQ=255)

                                                                     ttcCAGCATGTTTACTGTCACAGGCGTTAAGACCTAAAACTGTCCCCGTTTCGCCTATGCGTTCTCTTCCACCATTTCCGTTATGTGcaacaa                    >  1:246991/1‑93 (MQ=255)

                                                                      tcCAGCATGTTTACTGTCACAGGCGTTAAGACCTAAAACTGTCCCCGTTTCGCCTATGCGTTCTCTTCCACCATTTCCGTTATGTGCaacaac                   <  1:1712250/93‑1 (MQ=255)

                                                                      tcCAGCATGTTTACTGTCACAGGCGTTAAGACCTAAAACTGTCCCCGTTTCGCCTATGCGTTCTCTTCCACCATTTCCGTTATGTGCaacaac                   <  1:4660751/93‑1 (MQ=255)

                                                                        cAGCATGTTTACTGTCACGGGCGTTAAGACCTAAAACTGTCCCCGTTTCGCCTATGCGTTCTCTTCCACCATTTCCGTTATGTGCAACAACtt                 <  1:4531491/93‑1 (MQ=255)

                                                                        cAGCATGTTTACTGTCACAGGCGTTAAGACCTAAAACTGTCCCCGTTTCGCCTATGCGTTCTCTTCCACCATTTCCGTTATGTGCAACAACtt                 <  1:334695/93‑1 (MQ=255)

                                                                          gCATGTTTACTGTCACAGGCGTTAAGACCTAAAACTGTCCCCGTTTCGCCTATGCGTTCTCTTCCACCATTTCCGTTATGTGCAACAACTTGa               >  1:197371/1‑93 (MQ=255)

                                                                          gCATGTTTACTGTCACAGGCGTTAAGACCTAAAACTGTCCCCGTTTCGCCTATGCGTTCTCTTCCACCATTTCCGTTATGTGCAACAACTTGa               >  1:4117506/1‑93 (MQ=255)

                                                                           cATGTTTACTGTCACAGGCGTTAAGACCTAAAACTGTCCCCGTTTCGCCTATGCGTTCTCTTCCACCATTTCCGTTATGTGCAACAACTTGAt              <  1:3967002/93‑1 (MQ=255)

                                                                            aTGTTTACTGTCACAGGCGTTAAGACCTAAAACTGTCCCCGTTTCGCCTATGCGTTCTCTTCCACCATTTCCGTTATGTGCAACAACTTGAtt             <  1:1682658/93‑1 (MQ=255)

                                                                             tGTTTACTGTCACAGGCGTTAAGACCTAAAACTGTCCCCGTTTCGCCTATGCGTTCTCTTCCACCATTTCCGTTATGTGCAACAACTTGAttt            >  2:1582908/1‑93 (MQ=255)

                                                                             tGTTTACTGTCACAGGCGTTAAGACCTAAAACTGTCCCCGTTTCGCCTATGCGTTCTCTTCCACCATTTCCGTTATGTGCAACAACTTGAttt            >  2:4167039/1‑93 (MQ=255)

                                                                              gTTTACTGTCACAGGCGTTAAGACCTAAAACTGTCCCCGTTTCGCCTATGCGTTCTCTTCCACCATTTCCGTTATGTGCAACAACTTGATTta           >  1:3288526/1‑93 (MQ=255)

                                                                              gTTTACTGTCACAGGCGTTAAGACCTAAAACTGTCCCCGTTTCGCCTATGCGTTCTCTTCCACCATTTCCGTTATGTGCAACAACTTGATTta           >  2:4686843/1‑93 (MQ=255)

                                                                              gTTTACTGTCACAGGCGTTAAGACCTAAAACTGTCCCCGTTTCGCCTATGCGTTCTCTTCCACCATTTCCGTTATGTGCAACAACTTGATTta           >  2:4096437/1‑93 (MQ=255)

                                                                              gTTTACTGTCACAGGCGTTAAGACCTAAAACTGTCCCCGTTTCGCCTATGCGTTCTCTTCCACCATTTCCGTTATGTGCAACAACTTGATTta           >  2:3987900/1‑93 (MQ=255)

                                                                               tttACTGTCACGGGCGTTAAGACCTAAAACTGTCCCCGTTTCGCCTATGCGTTCTCTTCCACCATTTCCGTTATGTGCAACAACTTGATTtat          <  1:2330476/93‑1 (MQ=255)

                                                                                ttACTGTCACAGGCGTTAAGACCTAAAACTGTCCCCGTTTCGCCTATGCGTTCTCTTCCACCATTTCCGTTATGTGCAACAACTTGATTtata         <  1:3756411/93‑1 (MQ=255)

                                                                                 tACTGTCACAGGCGTTAAGACCTAAAACTGTCCCCGTTTCGCCTATGCGTTCTCTTCCACCATTTCCGTTATGTGCAACAACTTGATTTATAc        <  1:1768940/93‑1 (MQ=255)

                                                                                 tACTGTCACAGGCGTTAAGACCTAAAACTGTCCCCGTTTCGCCTATGCGTTCTCTTCCACCATTTCCGTTATGTGCAACAACTTGATTTATAc        >  2:3038856/1‑93 (MQ=255)

                                                                                  aCTGTCACAGGCGTTAAGACCTAAAACTGTCCCCGTTTCGCCTATGCGTTCTCTTCCACCATTTCCGTTATGTGCAACAACTTGATTTATAcc       >  2:273723/1‑93 (MQ=255)

                                                                                   cTGTCACAGGCGTTAAGACCTAAAACTGTCCCCGTTTCGCCTATGCGTTCTCTTCCACCATTTCCGTTATGTGCAACAACTTGATTTATACCt      <  2:4246425/93‑1 (MQ=255)

                                                                                   cTGTCACAGGCGTTAAGACCTAAAACTGTCCCCGTTTCGCCTATGCGTTCTCTTCCACCATTTCCGTTATGTGCAACAACTTGATTTATACCt      >  1:1544058/1‑93 (MQ=255)

                                                                                     gTCACAGGCGTTAAGACCTAAAACTGTCCCCGTTTCGCCTATGCGTTCTCTTCCACCATTTCCGTTATGTGCAACAACTTGATTTATACCTaa    >  2:3414506/1‑93 (MQ=255)

                                                                                     gTCACAGGCGTTAAGACCTAAAACTGTCCCCGTTTCGCCTATGCGTTCTCTTCCACCATTTCCGTTATGTGCAACAACTTGATTTATACCTaa    >  1:3797512/1‑93 (MQ=255)

                                                                                      tCACAGGCGTTAAGACCTAAAACTGTCCCCGTTTCGCCTATGCGTTCTCTTCCACCATTTCCGTTATGTGCAACAACTTGATTTATACCTAAc   >  1:532982/1‑93 (MQ=255)

                                                                                       cacaGGCGTTAAGACCTAAAACTGTCCCCGTTTCGCCTATGCGTTCTCTTCCACCATTTCCGTTATGTGCAACAACTTGATTTATACCTAACa  >  2:3593579/1‑93 (MQ=255)

                                                                                       cacaGGCGTTAAGACCTAAAACTGTCCCCGTTTCGCCTATGCGTTCTCTTCCACCATTTCCGTTATGTGCAACAACTTGATTTATACCTAACa  <  1:2097674/93‑1 (MQ=255)

ACTTCCAGGGAGTGGTTATCTCAGGCCCTGCACCAGGGTTAATTTGGCAATTATGCATGCTGAACATTCTTCCAGCATGTTTACTGTCACGGGCGTTAAGACCTAAAACTGTCCCCGTTTCGCCTATGCGTTCTCTTCCACCATTTCCGTTATGTGCAACAACTTGATTTATACCTAACA  >  NC_000913/3628669‑3628848

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 26 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`