BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,634,228	A→G	R271R (CGT→CGC)	yhiN ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,634,228	0	A	G	95.2%	478.3 / 7.5	166	R271R (CGT→CGC)	yhiN	hypothetical protein
Reads supporting (aligned to +/- strand): ref base A (7/1); new base G (70/88); total (77/89)
Fisher's exact test for biased strand distribution p-value = 2.54e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.62e-01

Only 100 of 175 total aligned reads displayed.

ATTTGCCCGAGTTGCTGTAAGCGTTCAACCAACCGCTTCGGTAGATGAACCGCCAGTGTGTTTTTCAGGCTTTGATTCGGATGTGCGTTACGCTGCTCATTCAGGAAGGTTTCGAGGTCCACATCCGGTAGCAGATTGATGCTGACAAATTCCCCCGGTTGCCAGTAGCTTGAAATC  >  NC_000913/3634139‑3634315

aTTTGCCCGAGTTGCTGTAAGCGTTCAACCAACCGCTTCGGTAGATGAACCGCCAGTGTGTTTTTCAGGCTTTGATTCGGATGTGCGTTgcgc                                                                                      <  2:1392349/93‑1 (MQ=255)

 tttGCCCGAGTTGCTGTAAGCGTTCAACCAACCGCTTCGGTAGATGAACCGCCAGTGGGTTTTTCAGGCTTTGATTCGGATGTGCGTTGCgct                                                                                     <  2:4336711/93‑1 (MQ=255)

  ttGCCCGAGTTGCTGTAAGCGTTCAACCAACCGCTTCGGTAGATGAACCGCCAGTGTGTTTTTCAGGCTTTGATTCGGATGTGCGTTGCgctg                                                                                    >  2:132785/1‑93 (MQ=255)

   tGCCCGAGTTGCTGTAAGAGTTCAACCAACCGCTTCGGTAGATGAACCGCCAGTGTGTTTTTCAGGCTTTGATTCGGATGTGCGTTGCgctgc                                                                                   <  2:2758862/93‑1 (MQ=255)

    gCCCGAGTTGCTGTAAGCGTTCAACCAACCGCTTCGGTAGATGAACCGCCAGTGTGTTTTTCAGGCTTTGATTCGGATGTGCGTTGCgctgct                                                                                  <  1:2952613/93‑1 (MQ=255)

     cccGAGTTGCTGTAAGCGTTCAACCAACCGCTTCGGTTGATGAATCGCCAGTGTGTGTTTCAGGCTTTGATTCGGATGTGCTTTGCGCTGCTc                                                                                 >  2:4209312/1‑93 (MQ=255)

     cccGAGTTGCTGTAAGCGTTCAACCAACCGCTTCGGTAGATGAACCGCCAGTGTGTTTTTCAGGCTTTGATTCGGATGTGCGTTGCGCTGCTc                                                                                 >  2:3440313/1‑93 (MQ=255)

     cccGAATTGCTGTAAGCGTTCAACCAACCGCTTCGGGAGATGAACCGCCAGTGGGTTTTTCAGGCTTTGATTCGGATGTGCGCTGCGCTGCTc                                                                                 >  1:2778997/1‑93 (MQ=255)

      ccGAGTTGCTGTAAGCGTTCAACCAACCGCTTCGGTAGATGAACCGCCAGTGTGTTTTTCAGGCTTTGATTCGGATGTGCGTTGCGCTGCTCa                                                                                <  1:4158595/93‑1 (MQ=255)

       cGAGTTGCTGTAAGCGTTCAACCAACCGCTTCGGTAGATGAACCGCCAGTGTGTTTTTCAGGCTTTGATTCGGATGTGCGTTGCGCTGCTCAt                                                                               <  1:858529/93‑1 (MQ=255)

             gCTGTAAGCGTTCAACCAACCGCTTCGGTAGATGAACCGCCAGTGTGTTTTTCAGGCTTTGATTCGGATGTGCGTTGCGCTGCTCATTCAGGa                                                                         <  2:3181903/93‑1 (MQ=255)

              cTGTAAGCGTTCAACCAACCGCTTCGGTAGATGATCCGCCAGTGTGTTTTTCAGGCTTTGATTCGGATGTTCGTTGCGCTGCTCATTCAGGaa                                                                        <  2:751456/93‑1 (MQ=255)

              cTGTAAGCGTTCAACCAACCGCTTCGGTAGATGAACCGCCAGTGTGTTTTTCAGGCTTTGATTCGGATGTGCGTTGCGCTGCTCATTCAGGaa                                                                        <  1:3865924/93‑1 (MQ=255)

                 tAAGCGTTCAACCAACCGCTTCGGTAGATGAACCGCCAGTGTGTTTTTCAGGCTTTGATTCGGATGTGCGTTGCGCTGCTCATTCAGGAAGGt                                                                     >  2:1474352/1‑93 (MQ=255)

                 tAAGCGTTCAACCAACCGCTTCGGTAGATGAACCGCCAGTGTGTTTTTCAGGCTTTGATTCGGATGTGCGTTGCGCTGCTCATTCAGGAAGGt                                                                     >  1:3321789/1‑93 (MQ=255)

                 gaaGCGTTCAGCGAACCGCTTCGGTAGATGAACCGCCAGTGTGTTTTTCAGGCTTTGATTCGGATGTGCGTTGCGCTGCTCATTCAGGAAGGt                                                                     <  1:68567/92‑1 (MQ=255)

                  aaGCGTTCAACCAACCGCTTCGGTAGATGAACCGCCAGTGTGTTTTTCAGGCTTTGATTCGGATGTGCGTTGCGCTGCTCATTCAGGAAGGtt                                                                    >  1:928869/1‑93 (MQ=255)

                  aaGCGTTCAACCAACCGCTTCGGTAGATGAACCGCCAGTGTGTTTTTCAGGCTTTGATTCGGATGTGCGTTGCGCTGCTCATTCAGGAAGGtt                                                                    <  1:3878977/93‑1 (MQ=255)

                   aGCGTTCAACCAACCGCTTCGGTAGATGAACCGCCAGTGTGTTTTTCAGGCTTTGATTCGGATGTGCGTTGCGCTGCTCATTCAGGAAGGttt                                                                   >  1:1807213/1‑93 (MQ=255)

                   aGCGTTCAACCAACCGCTTCGGTAGATGAACCGCCAGTGTGTTTTTCAGGCTTTGATTCGGATGTGCGTTACGCTGCTCATTCAGGAAGGttt                                                                   >  2:2435334/1‑93 (MQ=255)

                    gCGTTCAACCAACCGCTTCGGTAGATGAACCGCCAGTGTGTTTTTCAGGCTTTGATTCGGATGTGCGTTGCGCTGCTCATTCAGGAAGGTTTc                                                                  <  1:4095938/93‑1 (MQ=255)

                    gCGTTCAACCAACCGCTTCGGTAGATGAACCGCCAGTGTGTTTTTCAGGCTTTGATTCGGATGTGCGTTGCGCTGCTCATTCAGGAAGGTTTc                                                                  <  2:4516427/93‑1 (MQ=255)

                    gCGTTCAACCAACCGCTTCGGTAGATGAACCGCCAGTGTGTTTTTCAGGCTTTGATTCGGATGTGCGTTGCGCTGCTCATTCAGGAAGGTTTc                                                                  <  1:1389880/93‑1 (MQ=255)

                      gTTCAACCAACCGCTTCGGTAGATGAACCGCCAGTGTGTTTTTCAGGCTTTGATTCGGATGTGCGTTGCGCTGCTCATTCAGGAAGGTTTCGa                                                                >  1:262494/1‑93 (MQ=255)

                       ttCAACCAACCGCTTCGGTAGATGAACCGCCAGTGTGTTTTTCAGGCTTTGATTCGGATGTGCGTTGCGCTGCTCATTCAGGAAGGTTTCGAg                                                               >  1:3029809/1‑93 (MQ=255)

                       ttCAACCAACCGCTTCGGTAGATGAACCGCCAGTGTGTTTTTCAGGCTTTGATTCGGATGTGCGTTGCGCTGCTCATTCAGGAAGGTTTCGAg                                                               >  2:2846940/1‑93 (MQ=255)

                       ttCAACCAACCGCTTCGGTAGATGAACCGCCAGTGTGTTTTTCAGGCTTTGATTCGGATGTGCGTTACGCTGCTCATTCAGGAAGGTTTCGAg                                                               >  2:4317952/1‑93 (MQ=255)

                        tCAACCAGCCGCTTCGGTAGATGATCCGCCAGTGTGTTTTTCAGGCTTTGATTCGGATGTGCGTTGCGCTGCTCATTCAGGAAGGTTTCGAgg                                                              <  2:2073988/93‑1 (MQ=255)

                        tCAACCAACCGCTTCGGTAGATGAACCGCCAGTGTGTTTTTCAGGCTTTGATTCGGATGTGCGTTGCGCTGCTCATTCAGGAAGGTTTCGAgg                                                              <  1:4465735/93‑1 (MQ=255)

                         gaaccatccGCTTCGGTAGATGAACCGCCAGTGTGTTTTTCAGGCTTTGATTCGGATGTGCGTTGCGCTGCTCATTCAGGAAGGTTTCGAGGt                                                             <  1:3225221/92‑1 (MQ=255)

                         caaccaacCGCTTCGGTAGATGAACCGCCAGTGTGTTTTTCAGGCTTTGATTCGGATGTGCGTTGCGCTGCTCATTCAGGAAGGTTTCGAGGt                                                             <  1:3225222/93‑1 (MQ=255)

                         caaccaacCGCTTCGGTAGATGAACCGCCAGTGTGTTTTTCAGGCTTTGATTCGGATGTGCGTTGCGCTGCTCATTCAGGAAGGTTTCGAGGt                                                             <  2:3797504/93‑1 (MQ=255)

                         caaccaacCGCTTCGGTAGATGAACCGCCAGTGTGTTTTTCAGGCTTTGATTCGGATGTGCGTTACGCTGCTCATTCAGGAAGGTTTCGAGGt                                                             >  2:1553394/1‑93 (MQ=255)

                           accaaccGCTTCGGTAGATGAACCGCCAGTGTGTTTTTCAGGCTTTGATTCGGATGTGCGTTGCGCTGCTCATTCAGGAAGGTTTCGAGGTcc                                                           <  1:2741248/93‑1 (MQ=255)

                           accaaccGCTTCGGTAGATGAACCGCCAGTGTGTTTTTCAGGCTTTGATTCGGATGTGCGTTGCGCTGCTCATTCAGGAAGGTTTCGAGGTcc                                                           <  1:1982563/93‑1 (MQ=255)

                            ccaaccGCTTCGGTAGATGAACCGCCAGTGTGTTTTTCAGGCTTTGATTCGGATGTGCGTTGCGCTGCTCATTCAGGAAGGTTTCGAGGTCca                                                          >  2:3852489/1‑93 (MQ=255)

                              caccGCTTCGGTAGACGAACCGGCAGTGTGTTTTTCAGGCTTTGATTCGGATGTGCGTTGCGCTGCTCATTCAGGAAGGTTTCGAGGTCcaca                                                        <  2:258287/92‑1 (MQ=255)

                              acccGTTTAGGGAGATGAACCGCCAGTGTGTTTTTAAGGCTTTGATTCGGATGTGCGTTGCGCTGCTCATTCAGGAAGGTTTCGAGGTCcaca                                                        <  2:874391/91‑1 (MQ=255)

                              aaccGCTTCGGTAGATGAACCGCCAGTGTGTTTTTCAGGCTTTGATTCGGATGTGCGTTGCGCTGCTCATTCAGGAAGGTTTCGAGGTCcaca                                                        >  2:3536940/1‑93 (MQ=255)

                              aaccGCTTCGGTAGATGAACCGCCAGTGTGTTTTTCAGGCTTTGATTCGGATGTGCGTTGCGCTGCTCATTCAGGAAGGTTTCGAGGTCcaca                                                        <  2:4572639/93‑1 (MQ=255)

                              aaccGCTTCGGTAGATGAACCGCCAGTGTGTTTTTCAGGCTTTGATTCGGATGTGCGTTGCGCTGCTCATTCAGGAAGGTTTCGAGGTCcaca                                                        <  1:3674760/93‑1 (MQ=255)

                               accGCTTCGGTAGATGAACCGCCAGTGTGTTTTTCAGGCTTTGATTCGGATGTGCGTTGCGCTGCTCATTCAGGAAGGTTTCGAGGTCCACAt                                                       <  1:1034949/93‑1 (MQ=255)

                                ccGCTTCGGTAGATGAACCGCCAGTGTGTTTTTCAGGCTTTGATTCGGATGTGCGTTGCGCTGCTCATTCAGGAAGGTTTCGAGGTCCACATc                                                      <  2:511608/93‑1 (MQ=255)

                                 cGCTTCGGTAGATGAACCGCCAGTGTGTTTTTCAGGCTTTGATTCGGATGTGCGTTGCGCTGCTCATTCAGGAAGGTTTCGAGGTCCACATcc                                                     <  2:325721/93‑1 (MQ=255)

                                  gCTTCGGTAGATGAACCGCCAGTGTGTTTTTCAGGCTTTGATTCGGATGTGCGTTGCGCTGCTCATTCAGGAAGGTTTCGAGGTCCACATCCg                                                    >  1:1470047/1‑93 (MQ=255)

                                  gCTTCGGTAGATGAACCGCCAGTGTGTTTTTCAGGCTTTGATTCGGATGTGCGTTGCGCTGCTCATTCAGGAAGGTTTCGAGGTCCACATCCg                                                    >  2:3657769/1‑93 (MQ=255)

                                  gCTTCGGTAGATGAACCGCCAGTGTGTTTTTCAGGCTTTGATTCGGATGTGCGTTGCGCTGCTCATTCAGGAAGGTTTCGAGGTCCACATCCg                                                    >  1:3747002/1‑93 (MQ=255)

                                  gCTTCGGTAGATGAACCGCCAGTGTGTTTTTCAGGCTTTGATTCGGATGTGCGTTGCGCTGCTCATTCAGGAAGGTTTCGAGGTCCACATCCg                                                    >  1:1948830/1‑93 (MQ=255)

                                  gCTTCGGTAGATGAACCGCCAGTGTGTTTTTCAGGCTTTGATTCGGATGTGCGTTGCGCTGCTCATTCACGAAGGTTTCGAGGTCCACATCCg                                                    >  2:3239342/1‑93 (MQ=255)

                                   cTTCGGTAGATGAACCGCCAGTGTGTTTTTCAGGCTTTGATTCGGATGTGCGTTGCGCTGCTCATTCAGGAAGGTTTCGAGGTCCACATCCgg                                                   <  2:2904544/93‑1 (MQ=255)

                                     tCGGTAGATGAACCGCCAGTGTGTTTTTCAGGCTTTGATTCGGATGTGCGTTGCGCTGCTCATTCAGGAAGGTTTCGAGGTCCACATCCGGTa                                                 >  2:639375/1‑93 (MQ=255)

                                     tCGGTAGATGAACCGCCAGTGTGTTTTTCAGGCTTTGATTCGGATGTGCGTTGCGCTGCTCATTCAGGAAGGTTTCGAGGTCCACATCCGGTa                                                 <  1:3826935/93‑1 (MQ=255)

                                      cGGTAGATGAACCGCCAGTGTGTTTTTCAGGCTTTGATTCGGATGTGCGTTGCGCTGCTCATTCAGGAAGGTTTCGAGGTCCACATCCGGTAg                                                >  2:465379/1‑93 (MQ=255)

                                        gTAGATGAACCGCCAGTGTGTTTTTCAGGCTTTGATTCGGATGTTCGTTGCGCTGCTCATTCAGGAAGGTTTCGAGGTCCACATCCGGTAGCa                                              <  1:3581370/93‑1 (MQ=255)

                                        gTAGATGAACCGCCAGTGTGTTTTTCAGGCTTTGATTCGGATGTGCGTTGCGCTGCTCATTCAGGAAGGTTTCGAGGTCCACATCCGGTAGCa                                              <  1:603667/93‑1 (MQ=255)

                                         tAGATGAACCGCCAGTGTGTTTTTCAGGCTTTGATTCGGATGTGCGTTGCGCTGCTCATTCAGGAAGGTTTCGAGGTCCACATCCGGTAGCAg                                             >  1:1459127/1‑93 (MQ=255)

                                          aGATGAACCGCCAGTGTGTTTTTCAGGCTTTGATTCGGATGTGCGTTGCGCTGCTCATTCAGGAAGGTTTCGAGGTCCACATCCGGTAGCAGa                                            <  2:4134488/93‑1 (MQ=255)

                                          aGATGAACCGCCAGTGTGTTTTTCAGGCTTTGATTCGGATGTGCGTTGCGCTGCTCATTCAGGAAGGTTTCGAGGTCCACATCCGGTAGCAGa                                            <  1:3924697/93‑1 (MQ=255)

                                            gtGAACCGCCAGTGTGTTTTTCAGGCTTTGATTCGGATGTGCGTTGCGCTGCTCATTCAGGAAGGTTTCGAGGTCCACATCCGGTAGCAGAtt                                          <  1:1252228/92‑1 (MQ=255)

                                            gtGAACCGCCAGTGTGTTTTTCAGGCTTTGATTCGGATGTGCGTTGCGCTGCTCATTCAGGAAGGTTTCGAGGTCCACATCCGGTAGCAGAtt                                          <  1:2871594/92‑1 (MQ=255)

                                            aTGAACCGCCAGTGTGTTTTTCAGGCTTTGATTCGGATGTGCGTTGCGCTGCTCATTCAGGAAGGTTTCGAGGTCCACATCCGGTAGCAGAtt                                          <  2:158579/93‑1 (MQ=255)

                                              gAACCGCCAGTGTGTTTTTCAGGCTTTGATTCGGATGTGCGTTGCGCTGCTCATTCAGGAAGGTTTTGAGGTCCACATCCGGTAGCAGATTGa                                        >  1:4029025/1‑93 (MQ=255)

                                              gAACCGCCAGTGTGTTTTTCAGGCTTTGATTCGGATGTGCGTTGCGCTGCTCATTCAGGAAGGTTTCGAGGTCCACATCCGGTAGCAGATTGa                                        >  2:1527568/1‑93 (MQ=255)

                                              gAACCGCCAGTGTGTTTTTCAGGCTTTGATTCGGATGTGCGTTGCGCTGCTCATTCAGGAAGGTTTCGAGGTCCACATCCGGTAGCAGATTGa                                        <  2:1771040/93‑1 (MQ=255)

                                                 ccGCCAGTGTGTTTTTCAGGCTTTGATTCGGATGTGCGTTGCGCTGCTCATTCAGGAAGGTTTCGAGGTCCACATCCGGTAGCAGATTGATGc                                     <  1:4632316/93‑1 (MQ=255)

                                                  cGCCAGTGTGTTTTTCAGGCTTTGATTCGGATGTGCGTTGCGCTGCTCATTCAGGAAGGTTTCGAGGTCCACATCCGGTAGCAGATTGATGCt                                    <  1:1192554/93‑1 (MQ=255)

                                                   gCCAGTGTGTTTTTCAGGCTTTGATTCGGATGTGCGTTGCGCTGCTCATTCAGGAAGGTTTCGAGGTCCACATCCGGTAGCAGATTGATGCTg                                   <  2:1793322/93‑1 (MQ=255)

                                                   cacAGTGTGTTTTTCAGGCTTTGATTCGGATGTGCGTTGCGCTGCTCATTCAGGAAGGTTTCGAGGTCCACAGCCGGTAGCAGATTGATGCTg                                   <  2:240805/91‑1 (MQ=255)

                                                     cAGTGTGTTTTTCAGGCTTTGATTCGGATGTGCGTTGCGCTGCTCATTCAGGAAGGTTTCGCGGTCCACATCCGGTAGCAGATTGATGCTGAc                                 >  1:4672312/1‑93 (MQ=255)

                                                     cAGTGTGTTTTTCAGGCTTTGATTCGGATGTGCGTTGCGCTGCTCATTCAGGAAGGTTTCGAGGTCCACATCCGGTAGCAGATTGATGCTGAc                                 >  2:2105017/1‑93 (MQ=255)

                                                     cAGTGTGTTTTTCAGGCTTTGATTCGGATGTGCGTTGCGCTGCTCATTCAGGAAGGTTTCGAGGTCCACATCCGGTAGCAGATTGATGCTGAc                                 >  2:1355827/1‑93 (MQ=255)

                                                      aGTGTGTTTTTCAGGCTTTGATTCGGATGTGCGTTGCGCTGCTCATTCAGGAAGGTTTCGAGGTCCACATCCGGTAGCAGATTGATGCTGACa                                >  1:1694189/1‑93 (MQ=255)

                                                      aGTGTGTTTTTCAGGCTTTGATTCGGATGTGCGTTGCGCTGCTCATTCAGGAAGGTTTCGAGGTCCACATCCGGTAGCAGATTGATGCTGACa                                <  2:3016476/93‑1 (MQ=255)

                                                       gtgtgtTTTTCAGGCTTTGATTCGGATGTGCGTTGCGCTGCTCATTCAGGAAGGTTTCGAGGTCCACATCCGGTAGCAGATTGATGCTGACaa                               >  2:4267340/1‑93 (MQ=255)

                                                          tgtTTTTCAGGCTTTGATTCGGATGTGCGTTGCGCTGCTCATTCAGGAAGGTTTCGAGGTCCACATCCGGTAGCAGATTGATGCTGACAAAtt                            >  1:3641034/1‑93 (MQ=255)

                                                           gtTTTTCAGGCTTTGATTCGGATGTGCGTTGCGCTGCTCATTCAGGAAGGTTTCGAGGTCCACATCCGGTAGCAGATTGATGCTGACAAATTc                           <  1:1012054/93‑1 (MQ=255)

                                                            tttttCAGGCTTTGATTCGGATGTGCGTTGCGCTGCTCATTCAGGAAGGTTTCGAGGTCCACATCCGGTAGCAGATTGATGCTGACAAATTcc                          >  2:1485552/1‑93 (MQ=255)

                                                              tttCAGGCTTTGATTCGGATGTGCGTTGCGCTGCTCATTCAGGAAGGTTTCGAGGTCCACATCCGGTAGCAGATTGATGCTGACAAATTccgc                        >  2:2421695/1‑91 (MQ=255)

                                                              tttCAGGCTTTGATTCGGATGTGCGTTGCGCTGCTCATTCAGGAAGGTTTCGAGGTCCACATCCGGTAGCAGATTGATGCTGACAAATTcccc                        <  1:2355019/93‑1 (MQ=255)

                                                              tttCAGGCTTTGATTCGGATGTGCGTTGCGCTGCTCATTCAGGAAGGTTTCGAGGTCCACATCCGGTAGCAGATTGATGCTGACAAATTcccc                        <  2:3562082/93‑1 (MQ=255)

                                                               ttCAGGCTTTGATTCGGATGTGCGTTGCGCTGCTAATTCAGGAAGGTTTCGAGGTCCACATCCGGTAGCAGATTGATGCTGACAAATTccccc                       >  1:345822/1‑93 (MQ=255)

                                                                tCAGGCTTTGATTCGGATGTGCGTTGCGCTGCTCATTCAGGAAGGTTTCGAGGTCCACATCCGGTAGCAGATTGATGCTGACAAATTCCCCCg                      <  2:498656/93‑1 (MQ=255)

                                                                tCAGGCTTTGATTCGGATGTGCGTTGCGCTGCTCATTCAGGAAGGTTTCGAGGTCCACATCCGGTAGCAGATTGATGCTGACAAATTCCCCCg                      <  1:3412902/93‑1 (MQ=255)

                                                                tCAGGCTTTGATTCGGATGTGCGTTGCGCTGCTCATTCAGGAAGGTTTCGAGGTCCACATCCGGTAGCAGATTGATGCTGACAAATTCCCCCg                      <  1:2491491/93‑1 (MQ=255)

                                                                 cAGGCTTTGATTCGGATGTGCGTTGCGCTGCTCATTCAGGAAGGTTTCGAGGTCCACATCCGGTAGCAGATTGATGCTGACAAATTCCCCCgg                     >  1:2091192/1‑93 (MQ=255)

                                                                  aGGCTTTGATTCGGATGTGCGTTGCGCTGCTCATTCAGGAAGGTTTCGAGGTCCACATCCGGTAGCAGATTGATGCTGACAAATTCCCCCGGt                    <  1:4187397/93‑1 (MQ=255)

                                                                  aGGCTTTGATTCGGATGTGCGTTGCGCTGCTCATTCAGGAAGGTTTCGAGGTCCACATCCGGTAGCAGATTGATGCTGACAAATTCCCCCGGt                    <  2:219795/93‑1 (MQ=255)

                                                                   ggCTTTGATTCGGATGTGCGTTGCGCTGCTCATTCAGGAAGGTTTCGAGGTCCACATCCGGTAGCAGATTGATGCTGACAAATTCCCCCGGtt                   <  1:3500502/93‑1 (MQ=255)

                                                                   ggCTTTGATTCGGATGTGCGTTGCGCTGCTCATTCAGGAAGGTTTCGAGGTCCACATCCGGTAGCAGATTGATGCTGACAAATTCCCCCGGtt                   <  1:1906523/93‑1 (MQ=255)

                                                                      tttGATTCGGATGTGCGTTGCGCTGCTCATTCAGGAAGGTTTCGAGGTCCACATCCGGTAGCAGATTGATGCTGACAAATTCCCCCGGTTGcc                >  2:2377364/1‑93 (MQ=255)

                                                                       ttGATTCGGATGTGCGTTACGCTGCTCATTCAGGAAGGTTTCGAGGTCCACATCCGGTAGCAGATTGATGCTGACAAATTCCCCCGGTTGCCa               >  1:1569872/1‑93 (MQ=255)

                                                                        tGATTCGGATGTGCGTTGCGCTGCTCATTCAGGAAGGTTTCGAGGTCCACATCCGGTAGCAGATTGATGCTGACAAATTCCCCCGGTTGCCAg              <  2:1836923/93‑1 (MQ=255)

                                                                         gATTCGGATGTGCGTTGCGCTGCTCCTTCAGGAAGGTTTCGAGCACAACATCCGGTAGCAGGTTGATGCTGACAAAATACGCCGGCTGCCAGt             >  1:4275126/1‑93 (MQ=255)

                                                                           ttCGGATGTGCGTTGCGCTGCTCATTCAGGAAGGTTTCGAGGTCCACATCCGGTAGCAGATTGATGCTGACAAATTCCCCCGGTTGCCAGTAg           >  1:658245/1‑93 (MQ=255)

                                                                             cGGATGTGCGTTGCGCTGCTCATTCAGGAAGGTTTCGAGGTCCACATCCGGTAGCAGATTGATGCTGACAAATTCCCCCGGTTGCCAGTAGCt         >  1:1785081/1‑93 (MQ=255)

                                                                              ggATGTGCGTTGCGCTGCTCATTCAGGAAGGTTTCGAGGTCCACATCCGGTAGCAGATTGATGCTGACAAATTCCCCCGGTTGCCAGTAGCtt        <  2:1606190/93‑1 (MQ=255)

                                                                               gATGTGCGTTGCGCTGCTCATTCAGGAAGGTTTCGAGGTCCACATCCGGTAGCAGATTGATGCTGACAAATTCCCCCGGTTGCCAGTAGCTTg       >  2:65754/1‑93 (MQ=255)

                                                                               gATGTGCGTTGCGCTGCTCATTCAGGAAGGTTTCGAGGTCCACATCCGGTAGCAGATTGATGCTGACAAATTCCCCCGGTTCCCAGTAGCTTg       >  1:2291703/1‑93 (MQ=255)

                                                                                 tgtgCGTTGCGCTGCTCATTCAGGAAGGTTTCGAGGTCCACATCCGGTAGCAGATTGATGCTGACAAATTCCCCCGGTTGCCAGTAGCTTGaa     >  1:4534352/1‑93 (MQ=255)

                                                                                    gCGTTGCGCTGCTCATTCAGGAAGGTTTCGAGGTCCACATCCGGTAGCAGATTGATGCTGACAAATTCCCCCGGTTGCCAGTAGCTTGAAATc  <  2:3830217/93‑1 (MQ=255)

ATTTGCCCGAGTTGCTGTAAGCGTTCAACCAACCGCTTCGGTAGATGAACCGCCAGTGTGTTTTTCAGGCTTTGATTCGGATGTGCGTTACGCTGCTCATTCAGGAAGGTTTCGAGGTCCACATCCGGTAGCAGATTGATGCTGACAAATTCCCCCGGTTGCCAGTAGCTTGAAATC  >  NC_000913/3634139‑3634315

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 18 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`