BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,649,511	G→A	E197E (GAG→GAA)	yhiS →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,649,511	0	G	A	98.7%	1046.3 / ‑1.3	297	E197E (GAG→GAA)	yhiS	hypothetical protein
Reads supporting (aligned to +/- strand): ref base G (2/2); new base A (154/139); total (156/141)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.74e-01

Only 100 of 303 total aligned reads displayed.

TAATTTTATTGCTGAACAACGAGCTGACTTACCAACTAATATAAAAGATATTCAAAGCCTGTATCAAAAAATGACGAAGTTATATATTGAGCACAGTGAAAATAAAAACAGGATGAAAGTCTTTGCCGGAACCAACTTTATTGATTTCAATATGACAGGACAGAACCTGTCCGGGTTCGTTCT  >  NC_000913/3649421‑3649603

tAATTTTATTGCTGAACAACGAGCTGACTTACCAACTAATATAAAAGATATTCAAAGCCTGTATCAAAAAATGACGAAGTTATATATTGAAca                                                                                            >  2:3536817/1‑93 (MQ=255)

 aaTTTTATTGCTGAACAACGAGCTGACTTACCAACTAATATAAAAGATATTCAAAGCCTGTATCAAAAAATGACGAAGTTATATATTGAacac                                                                                           >  2:4100840/1‑93 (MQ=255)

  aTTTTATTGCTGAACAACGAGCTGACTTACCAACTAATATAAAAGATATTCAAAGCCTGTATCAAAAAATGACGAAGTTATATATTGAAcaca                                                                                          <  1:1252572/93‑1 (MQ=255)

  aTTTTATTGCTGAACAACGAGCTGACTTACCAACTAATATAAAAGATATTCAAAGCCTGTATCAAAAAATGACGAAGTTATATATTGAAcaca                                                                                          >  1:629145/1‑93 (MQ=255)

  aTTTTATTGCTGAACAACGAGCTGACTTACCAACTAATATAAAAGATATTCAAAGCCTGTATCAAAAAATGACGAAGTTATATATTGAAcaca                                                                                          >  1:4117176/1‑93 (MQ=255)

  aTTTTATTGCTGAACAACGAGCTGACTTACCAACTAATATAAAAGATATTCAAAGCCTGTATCAAAAAATGACGAAGTTATATATTGAAcaca                                                                                          <  2:4483550/93‑1 (MQ=255)

  aTTTTATTGCTGAACAACGAGCTGACTTACCAACTAATATAAAAGATATTCAAAGCCTGTATCAAAAAATGACGAAGTTATATATTGAAcaca                                                                                          <  1:2518036/93‑1 (MQ=255)

    tttATTGCTGAACAACGAGCTGACTTACCAACTAATATAAAAGATATTCAAAGCCTGTATCAAAAAATGACGAAGTTATATATTGAACACAGt                                                                                        >  2:1386643/1‑93 (MQ=255)

    tttATTGCTGAAAAACGAGCTGACTTACCAACTAATATAAAAGATATTCAAAGGCTGCATCAAAAAATGACGAAGTTATATATTGAACACAgg                                                                                        >  2:622968/1‑92 (MQ=255)

     ttATTGCTGAACAACGAGCTGACTTACCAACTAATATAAAAGATATTCAAAGCCTGTATCAAAAAATGACGAAGTTATATATTGAACACAGTg                                                                                       <  1:3479177/93‑1 (MQ=255)

     ttATTGCTGAACAACGAGCTGACTTACCAACTAATATAAAAGATATTCAAAGCCTGTATCAAAAAATGACGAAGTTATATATTGAACACAGTg                                                                                       >  2:1912322/1‑93 (MQ=255)

        ttGCTGAACAACGAGCTGACTTACCAACTAATATAAAAGATATTCAAAGCCTGTATCAAAAAATGACGAAGTTATATATTGAACACAGTGaaa                                                                                    <  2:1983280/93‑1 (MQ=255)

        ttGCTGAACAACGAGCTGACTTACCAACTAATATAAAAGATATTCAAAGCCTGTATCAAAAAATGACGAAGTTATATATTGAACACAGTGaaa                                                                                    >  2:583292/1‑93 (MQ=255)

          gCTGAACAACGAGCTGACTTACCAACTAATATAAAAGATATTCAAAGCCTGTATCAAAAAATGACGAAGTTATATATTGAACACAGTGAAAAt                                                                                  >  2:3519266/1‑93 (MQ=255)

          gCTGAACAACGAGCTGACTTACCAACTAATATAAAAGATATTCAAAGCCTGTATCAAAAAATGACGAAGTTATATATTGAACACAGTGAAAAt                                                                                  >  2:209221/1‑93 (MQ=255)

          gCTGAACAACGAGCTGACTTACCAACTAATATAAAAGATATTCAAAGCCTGTATCAAAAAATGACGAAGTTATATATTGAACACAGTGAAAAt                                                                                  >  1:3650349/1‑93 (MQ=255)

             gAACAACGAGCTGACTTACCAACTAATATAAAAGATATTCAAAGCCTGTATCAAAAAATGACGAAGTTATATATTGAACACAGTGAAAATaaa                                                                               <  2:2018310/93‑1 (MQ=255)

              aacaacGAGCTGACTTACCAACTAATATAAAAGATATTCAAAGCCTGTATCAAAAAATGACGAAGTTATATATTGAACACAGTGAAAATaaaa                                                                              >  1:2099496/1‑93 (MQ=255)

               acaacGAGCTGACTTACCAACTAATATAAAAGATATTCAAAGCCTGTATCAAAAAATGACGAAGTTATATATTGAACACAGTGAAAATaaaaa                                                                             >  1:2382128/1‑93 (MQ=255)

                caacGAGCTGACTTACCAACTAATATAAAAAATATTCAAAGCCTGTATCAAAAAATGACGAAGTTATATATTGAACACAGTGAAAATAAAAAc                                                                            <  2:3126596/93‑1 (MQ=255)

                 aacGAGCTGACTTACCAACTAATATAAAAGATATTCAAAGCCTGTATCAAAAAATGACGAAGTTATATATTGAGCACAGTGAAAATAAAAACa                                                                           <  1:4527602/93‑1 (MQ=255)

                 aacGAGCTGACTTACCAACTAATATAAAAGATATTCAAAGCCTGTATCAAAAAATGACGAAGTTATATATTGAACACAGTGAAAATAAAAACa                                                                           >  1:1917447/1‑93 (MQ=255)

                  acGAGCTGACTTACCAACTAATATAAAAGATATTCAAAGCCTGTATCAAAAAATGACGAAGTTATATATGGAACACAGTGAAAATAAAAACAg                                                                          >  2:3389293/1‑93 (MQ=255)

                   cGAGCTGACTTACCAACTAATATAAAAGATATTCAAAGCCTGTATCAAAAAATGACGAAGTTATATATTGAACACAGTGAAAATAAAAACAgg                                                                         <  2:3574890/93‑1 (MQ=255)

                   cGAGCTGACTTACCAACTAATATAAAAGATATTCAAAGCCTGTATCAAAAAATGACGAAGTTATATATTGAACACAGTGAAAATAAAAACAgg                                                                         <  2:2479121/93‑1 (MQ=255)

                    gAGCTGACTTACCAACTAATATAAAAGATATTCAAAGCCTGTATCAAAAAATGACGAAGTTATATATTGAACACAGTGAAAATAAAAACAGGa                                                                        >  2:3273943/1‑93 (MQ=255)

                    gAGCTGACTTACCAACTAATATAAAAGATATTCAAAGCCTGTATCAAAAAATGACGAAGTTATATATTGAACACAGTGAAAATAAAAACAGGa                                                                        <  2:399828/93‑1 (MQ=255)

                     aGCTGACTTACCAACTAATATAACAGATACTCAAAGCCTGTATCAAAAAATGACGAAGTTATATATTGAGCACAGTGAAAATAAAAACAGGAt                                                                       <  1:1562799/93‑1 (MQ=255)

                     aGCTGACTTACCAACTAATATAAAAGATATTCAAAGCCTGTATCAAAAAATGACGAAGTTATATATTGAACACAGTGAAAATAAAAACAGGAt                                                                       <  1:1802449/93‑1 (MQ=255)

                     aGCTGACTTACCAACTAATATAAAAGATATTCAAAGCCTGTATCAAAAAATGACGAAGTTATATATTGAACACAGTGAAAATAAAAACAGGAt                                                                       <  1:1410995/93‑1 (MQ=255)

                     aGCTGACTTACCAACTAATATAAAAGATATTCAAAGCCTGTATCAAAAAATGACGAAGTTATATATTGAACACAGTGAAAATAAAAACAGGAt                                                                       <  2:4592091/93‑1 (MQ=255)

                      gCTGACTTACCAACTAATATAAAAGATATTCAAAGCCTGTATCAAAAAATGACGAAGTTATATATTGAGCACAGTGAAAATAAAAACAGGATg                                                                      >  2:1589905/1‑93 (MQ=255)

                       cTGACTTACCAACTAATATAAAAGATATTCAAAGCCTGTATCAAAAAATGACGAAGTTATATATTGAACACAGTGAAAATAAAAACAGGATGa                                                                     <  2:3681073/93‑1 (MQ=255)

                        tGACTTACCAACTAATATAAAAGATATTCAAAGCCTGTATCAAAAAATGACGAAGTTATATATTGAACACAGTGAAAATAAAAACAGGATGaa                                                                    <  1:3277713/93‑1 (MQ=255)

                         gACTTACCAACTAATATAAAAGATATTCAAAGCCTGTATCAAAAAATGACGAAGTTATATATTGAACACAGTGAAAATAAAAACAGGATGaaa                                                                   <  2:879778/93‑1 (MQ=255)

                         gACTTACCAACTAATATAAAAGATATTCAAAGCCTGTATCAAAAAATGACGAAGTTATATATTGAACACAGTGAAAATAAAAACAGGATGaaa                                                                   <  1:245337/93‑1 (MQ=255)

                          aCTTACCAACTAATATAAAAGATATTCAAAGCCTGTATCAAAAAATGACGAAGTTATATATTGAACACAGTGAAAATAAAAGCAGGATGAAAg                                                                  <  2:2315760/93‑1 (MQ=255)

                          aCTTACCAACTAATATAAAAGATATTCAAAGCCTGTATCAAAAAATGACGAAGTTATATATTGAACACAGTGAAAATAAAAACAGGATGAAAg                                                                  <  2:2900649/93‑1 (MQ=255)

                            ttACCAACTAATATAAAAGATATTCAAAGCCTGTATCAAAAAATGACGAAGTTATATATTGAACACAGTGAAAATAAAAACAGGATGAAAGTc                                                                <  1:363028/93‑1 (MQ=255)

                             tACCAACTAATATAAAAGATATTCAAAGCCTGTATCAAAAAATGACGAAGTTATATATTGAACACAGTGAAAATAAAAACAGGATGAAAGTCt                                                               >  1:1278669/1‑93 (MQ=255)

                              aCCAACTAATATAAAAGATATTCAAAGCCTGTATCAAAAAATGACGAAGTTATATATTGAACACAGTGAAAATAAAAACAGGATGAAAGTCtt                                                              >  1:3024473/1‑93 (MQ=255)

                               ccAACTAATATAAAAGATATTCAAAGCCTGTATCAAAAAATGACGAAGTTATATATTGAACACAGTGAAAATAAAAACAGGATGAAAGTCttt                                                             <  1:4155019/93‑1 (MQ=255)

                               ccAACTAATATAAAAGATATTCAAAGCCTGTATCAAAAAATGACGAAGTTATATATTGAACACAGTGAAAATAAAAACAGGATGAAAGTCttt                                                             <  2:4562235/93‑1 (MQ=255)

                               ccAACTAATATAAAAGATATTCAAAGCCTGTATCAAAAAATGACGAAGTTATATATTGAACACAGTGAAAATAAAAACAGGATGAAAGTCttt                                                             <  2:2820314/93‑1 (MQ=255)

                                 aaCTAATATAAAAGATATTCAAAGCCTGTATCTAAAAATGACGAAGTTATATATTGAACACAGTTAAAATAAAAACAGGATGAAAGTCTTTGc                                                           >  1:4207292/1‑93 (MQ=255)

                                  aCTAATATAAAAGATATTCAAAGCCTGTATCAAAAAATGACGAAGTTATATATTGAACACAGTGAAAATAAAAACAGGATGAAAGTCTTTGcc                                                          >  1:1609705/1‑93 (MQ=255)

                                   cTAATATAAAAGATATTCAAAGCCTGTATCAAAAAATGACGAAGTTATATATTGAACACAGTGAAAATAAAAACAGGATGAAAGTCTTTGCCg                                                         >  2:4643558/1‑93 (MQ=255)

                                    tAATATAAAAGATATTCAAAGCCTGTATCAAAAAATGACGAAGTTATATATTGAACACAGTGAAAATAAAAACAGGATGCAAGTCTTTGCCgg                                                        >  1:389427/1‑93 (MQ=255)

                                    tAATATAAAAGATATTCAAAGCCTGTATCAAAAAATGACGAAGTTATATATTGAACACAGTGAAAATAAAAACAGGATGAAAGTCTTTGCCgg                                                        <  2:1078264/93‑1 (MQ=255)

                                      atatAAAAGATATTCAAAGCCTGTATCAAAAAATGACGAAGTTATATATTGAACACAGTGAGAATAAAAACAGGATGAAAGTCTTTGCCGGaa                                                      <  1:2248652/93‑1 (MQ=255)

                                      atatAAAAGATATTCAAAGCCTGTATCAAAAAATGACGAAGTTATATATTGAACACAGTGAAAATAAAAACAGGATGAAAGTCTTTGCCGGaa                                                      >  1:3216727/1‑93 (MQ=255)

                                       tataAAAGATATTCAAAGCCTGTATCAAAAAATGACGAAGTTATATATTGAACACAGTGAAAATAAAAACAGGATGAAAGTCTTTGCCGGAAc                                                     <  2:3752177/93‑1 (MQ=255)

                                       tataAAAGATATTCAAAGCCTGTATCAAAAAATGACGAAGTTATATATTGAACACAGTGAAAATAAAAACAGGATGAAAGTCTTTGCCGGAAc                                                     <  2:3846167/93‑1 (MQ=255)

                                          aaaaGATATTCAAAGCCTGTATCAAAAAATGACGAAGTTATATATTGAACACAGTGAAAATAAAAACAGGATGAAAGTCTTTGCCGGAACCaa                                                  >  2:1721736/1‑93 (MQ=255)

                                             aGATATTCAAAGCCTGTATCAAAAAATGACGAAGTTATATATTGAACACAGTGAAAATAAAAACAGGATGAAAGTCTTTGCCGGAACCAACtt                                               >  1:1161610/1‑93 (MQ=255)

                                             aGATATTCAAAGCCTGTATCAAAAAATGACGAAGTTATATATTGAACACAGTGAAAATAAAAACAGGATGAAAGTCTTTGCCGGAACCAACtt                                               >  2:1865986/1‑93 (MQ=255)

                                              gATATTCAAAGCCTGTATCAAAAAATGACGAAGTTATATATTGAACACAGTGAAAATAAAAACAGGATGAAAGTCTTTGCCGGAACCAACttt                                              >  2:554365/1‑93 (MQ=255)

                                               atatTCAAAGCCTGTATCAAAAAATGACGAAGTTATATATTGAACACAGTGAAAATAAAAACAGGATGAAAGTCTTTGCCGGAACCAACTTTa                                             <  1:1041227/93‑1 (MQ=255)

                                               atatTCAAAGCCTGTATCAAAAAATGACGAAGTTATATATTGAACACAGTGAAAATAAAAACAGGATGAAAGTCTTTGCCGGAACCAACTTTa                                             <  2:4109791/93‑1 (MQ=255)

                                                tatTCAAAGCCTGTATCAAAAAATGACGAAGTTATATATTGAACACAGTGAAAATAAAAACAGGATGAAAGTCTTTGCCGGAACCAACTTTAt                                            <  2:2203077/93‑1 (MQ=255)

                                                tatTCAAAGCCTGTATCAAAAAATGACGAAGTTATATATTGAACACAGTGAAAATAAAAACAGGATGAAAGTCTTTGCCGGAACCAACTTTAt                                            <  2:4564068/93‑1 (MQ=255)

                                                 atTCAAAGCCTGTATCAAAAAATGACGAAGTTATATATTGAACACAGTGAAAATAAAAACAGGATGAAAGTCTTTGCCGGAACCAACTTTAtt                                           <  2:1198413/93‑1 (MQ=255)

                                                  ttCAAAGCCTGTATCAAAAAATGACGAAGTTATATATTGAACACAGTGAAAATAAAAACAGGATGAAAGTCTTTGCCGGAACCAACTTTATTg                                          >  1:2314092/1‑93 (MQ=255)

                                                   tCAAAGCCTGTATCAAAAAATGACGAAGTTATATATTGAACACAGTGAAAATAAAAACAGGATGAAAGTCTTTGCCGGAACCAACTTTATTGa                                         >  1:946674/1‑93 (MQ=255)

                                                     aaaGCCTGTATCAAAAAATGACGAAGTTATATATTGAACACAGTGAAAATAAAAACAGGATGAAAGTCTTTGCCGGAACCAACTTTATTGAtt                                       <  2:3187852/93‑1 (MQ=255)

                                                      aaGCCTGTATCAAAAAATGACGAAGTTATATATTGAACACAGTGAAAATAAAAACAGGATGAAAGTCTTTGCCGGAACCAACTTTATTGAttt                                      <  1:1508281/93‑1 (MQ=255)

                                                       aGCCTGTATCAAAAAATGACGAAGTTATATATTGAACACAGTGAAAATAAAAACAGGATGAAAGTCTTTGCCGGAACCAACTTTATTGATTTc                                     <  1:4611423/93‑1 (MQ=255)

                                                        gCCTGTATCAAAAAATGACGAAGTTATATATTGAACACAGTGAAAATAAAAACAGGATGAAAGTCTTTGCCGGAACCAACTTTATTGATTTCa                                    >  1:3424753/1‑93 (MQ=255)

                                                        gCCTGTATCAAAAAATGACGAAGTTATATATTGAACACAGTGAAAATAAAAACAGGATGAAAGTCTTTGCCGGAACCAACTTTATTGATTTCa                                    >  2:3770489/1‑93 (MQ=255)

                                                          cTGTATCAAAAAATGACGAAGTTATATATTGAACACAGTGAAAATAAAAACAGGATGAAAGTCTTTGCCGGAACCAACTTTATTGATTTCAat                                  >  2:4321818/1‑93 (MQ=255)

                                                           tGTATCAAAAAATGACGAAGTTATATATTGAACACAGTGAAAATAAAAACAGGATGAAAGTCTTTGCCGGAACCAACTTTATTGATTTCAata                                 >  2:3965456/1‑93 (MQ=255)

                                                           tGTATCAAAAAATGACGAAGTTATATATTGAACACAGTGAAAATAAAAACAGGATGAAAGTCTTTGCCGGAACCAACTTTATTGATTTCAata                                 <  1:3394590/93‑1 (MQ=255)

                                                           tGTATCAAAAAATGACGAAGTTATATATTGAACACAGTGAAAATAAAAACAGGATGAAAGTCTTTGCCGGAACCAACTTTATTGATTTCAata                                 >  1:2212498/1‑93 (MQ=255)

                                                            gTATCAAAAAATGACGAAGTTATATATTGAACACAGTGAAAATAAAAACAGGATGAAAGTCTTTGCCGGAACCAACTTTATTGATTTCAatat                                <  2:3374476/93‑1 (MQ=255)

                                                             tATCAAAAAATGACGAAGTTATATATTGAACACAGTGAAAATAAAAACAGGATGAAAGTCTTTGCCGGAACCAACTTTATTGATTTCAATATg                               <  2:2689347/93‑1 (MQ=255)

                                                             tATCAAAAAATGACGAAGTTATATATTGAACACAGTGAAAATAAAAACAGGATGAAAGTCTTTGCCGGAACCAACTTTATTGATTTCAATATg                               <  1:4398470/93‑1 (MQ=255)

                                                                cAAAAAATGACGAAGTTATATATTGAACACAGTGAAAATAAAAACAGGATGAAAGTCTTTGCCGGAACCAACTTTATTGATTTCAATATgaca                            <  1:2812200/93‑1 (MQ=255)

                                                                cAAAAAATGACGAAGTTATATATTGAACACAGTGAAAATAAAAACAGGATGAAAGTCTTTGCCGGAACCAACTTTATTGATTTCAATATgaca                            <  1:3683555/93‑1 (MQ=255)

                                                                cAAAAAATGACGAAGTTATATATTGAACACAGTGAAAATAAAAACAGGATGAAAGTCTTTGCCGGAACCAACTTTATTGATTTCAATATgaca                            <  2:2180177/93‑1 (MQ=255)

                                                                 aaaaaaTGACGAAGTTATATATTGAACACAGTGAAAATAAAAACAGGATGAAAGTCTTTGCCGGAACCAACTTTATTGATTTCAATATgacag                           >  2:148959/1‑93 (MQ=255)

                                                                 aaaaaaTGACGAAGTTATATATTGAACACAGTGAAAATAAAAACAGGATGAAAGTCTTTGCCGGAACCAACTTTATTGATTTCAATATgacag                           <  1:2645342/93‑1 (MQ=255)

                                                                 aaaaaaTGACGAAGTTATATATTGAACACAGTGAAAATAAAAACAGGATGAAAGTCTTTGCCGGAACCAACTTTATTGATTTCAATATgacag                           <  2:713615/93‑1 (MQ=255)

                                                                    aaaTGACGAAGTTATATATTGAACACAGTGAAAATAAAAACAGGATGAAAGTCTTTGCCGGAACCAACTTTATTGATTTCAATATgacaggac                        <  1:734889/93‑1 (MQ=255)

                                                                    aaaTGACGAAGTTATATATTGAACACAGTGAAAATAAAAACAGGATGAAAGTCTTTGCCGGAACCAACTTTATAGATTTCAATATgacaggac                        >  1:4296650/1‑93 (MQ=255)

                                                                      aTGACGAAGTTATATATTGAACACAGTGAAAATAAAAACAGGATGAAAGTCTTTGCCGGAACCAACTTTATTGATTTCAATATgacaggacag                      >  1:467681/1‑93 (MQ=255)

                                                                      aTGACGAAGTTATATATTGAACACAGTGAAAATAAAAACAGGATGAAAGTCTTTGCCGGAACCAACTTTATTGATTTCAATATgacaggacag                      >  2:80567/1‑93 (MQ=255)

                                                                         aCGAAGTTATATATTGAACCCAGTGAAAATAAAAACAGGATGAAAGTCTTTGCCGGAACCAACTTTATTGATTTCAATATGACAGGACAGAAc                   <  2:1023487/93‑1 (MQ=255)

                                                                         aCGAAGTTATATATTGAACACAGTGAAAATAAAAACAGGATGAAAGTCTTTGCCGGAACCAACTTTATTGATTTCAATATGACAGGACAGAAc                   <  2:434901/93‑1 (MQ=255)

                                                                         aCGAAGTTATATATTGAACACAGTGAAAATAAAAACAGGATGAAAGTCTTTGCCGGAACCAACTTTATTGATTTCAATATGACAGGACAGAAc                   <  1:395946/93‑1 (MQ=255)

                                                                          cGAAGTTATATATTGAACACAGTGAAAATAAAAACAGGATGAAAGTCTTTGCCGGAACCAACTTTATTGATTTCAATATGACAGGACAGAAcc                  <  2:2734924/93‑1 (MQ=255)

                                                                             aGTTATATATTGAACACAGTGAAAATAAAAACAGGATGAAAGTCTTTGCCGGAACCAACTTTATTGATTTCAATATGACAGGACAGAACCTGt               >  2:3896176/1‑93 (MQ=255)

                                                                               ttATATATTGAACACAGTGAAAATAAAAACAGGATGAAAGTCTTTGCCGGAACCAACTTTATTGATTTCAATATGACAGGACAGAACCTGTcc             >  1:1673005/1‑93 (MQ=255)

                                                                               ttATATATTGAACACAGTGAAAATAAAAACAGGATGAAAGTCTTTGCCGGAACCAACTTTATTGATTTCAATATGACAGGACAGAACCTGTcc             >  1:886285/1‑93 (MQ=255)

                                                                               ttATATATTGAACACAGTGAAAATAAAAACAGGATGAAAGTCTTTGCCGGAACCAACTTTATTGATTTCAATATGACAGGACAGAACCTGTcc             >  2:1699244/1‑93 (MQ=255)

                                                                                    tatTGAACACAGTGAAAATAAAAACAGGATGAAAGTCTTTGCCGGAACCAACTTTATTGATTTCAATATGACAGGACAGAACCTGTCCGGgtt        <  1:3095588/93‑1 (MQ=255)

                                                                                    tatTGAACACAGTGAAAATAAAAACACGATGAAAGTCTTTGCCGGAACCAACTTTATTGATTTCAATATGACAGGACAGAACCTGTCCGGgtt        >  2:2408923/1‑93 (MQ=255)

                                                                                     atTGAACACAGTGAAAATAAAAACAGGATGAAAGTCTTTGCCGGAACCAACTTTATTGATTTCAATATGACAGGACAGAACCTGTCCGGgttc       >  1:2938056/1‑93 (MQ=255)

                                                                                     atTGAACACAGTGAAAATAAAAACAGGATGAAAGTCTTTGCCGGAACCAACTTTATTGATTTCAATATGACAGGACAGAACCTGTCCGGgttc       >  2:489284/1‑93 (MQ=255)

                                                                                      ttGAACACAGTGAAAATAAAAACAGGATGAAAGTCTTTGCCGGAACCAACTTTATTGATTTCAATATGACAGGACAGAACCTGTCCGGgttcg      >  2:4189965/1‑93 (MQ=255)

                                                                                      ttGAACACAGTGAAAATAAAAACAGGATGAAAGTCTTTGCCGGAACCAACTTTATTGATTTCAATATGACAGGACAGAACCTGTCCGGgttcg      >  1:523870/1‑93 (MQ=255)

TAATTTTATTGCTGAACAACGAGCTGACTTACCAACTAATATAAAAGATATTCAAAGCCTGTATCAAAAAATGACGAAGTTATATATTGAGCACAGTGAAAATAAAAACAGGATGAAAGTCTTTGCCGGAACCAACTTTATTGATTTCAATATGACAGGACAGAACCTGTCCGGGTTCGTTCT  >  NC_000913/3649421‑3649603

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`