BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,731,386	T→C	V13A (GTA→GCA)	xylH →	XylH

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,731,386	0	T	C	99.5%	592.7 / ‑5.2	185	V13A (GTA→GCA)	xylH	XylH
Reads supporting (aligned to +/- strand): ref base T (1/0); new base C (82/102); total (83/102)
Fisher's exact test for biased strand distribution p-value = 4.49e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.81e-01

Only 100 of 191 total aligned reads displayed.

TCATAACCTGACTCAGGAGCAGGTGATGGAAGCCGCATTGAGGAGCGAACATCATGTCGAAAAGCAATCCGTCTGAAGTGAAATTGGCCGTACCGACATCCGGTGGCTTCTCCG‑GGCTGAAATCACTGAATTTGCAGGTCTTCGTGATGATTGCAGCTATCATCGCAATCATGCTGTTCTTT  >  NC_000913/3731296‑3731477

tCATAACCTGACTCAGGAGCAGGTGATGGAAGCCGCATTGAGGAGCGAACATCATGTCGAAAAGCAATCCGTCTGAAGTGAAATTGGCCGCAc                                                                                            >  2:650991/1‑93 (MQ=255)

 cATAACCTGACTCAGGAGCAGGTGATGGAAGCCGCATTGAGGAGCGAACATCATGTCGAAAAGCAATCCGTCTGAAGTGAAATTGGCCGCAcc                                                                                           <  1:1091543/93‑1 (MQ=255)

    aaCCTGACTCAGGAGCAGGTGATGGAAGCCGCATTGAGGAGCGAACATCATGTCGAAAAGCAATCCGTCTGAAGTGAAATTGGCCGCACCGAc                                                                                        >  1:3629680/1‑93 (MQ=255)

     aCCTGACTCAGGAGCAGGTGATGGAAGCCGCATTGAGGAGCGAACATCATGTCGAAAAGCAATCCGTCTGAAGTGAAATTGGCCGCACCGACa                                                                                       <  1:3805965/93‑1 (MQ=255)

     aCCTGACTCAGGAGCAGGTGATGGAAGCCGCATTGAGGAGCGAACATCATGTCGAAAAGCAATCCGTCTGAAGTGAAATTGGCCGCACCGACa                                                                                       >  2:4294311/1‑93 (MQ=255)

       cTGACTCAGGAGCAGGTGATGGAAGCCGCATTGAGGAGCGAACATCATGTCGAAAAGCAATCCGTCTGAAGTGAAATTGGCCGCACCGACATc                                                                                     <  2:2817173/93‑1 (MQ=255)

       cTGACTCAGGAGCAGGTGATGGAAGCCGCATTGAGGAGCGAACATCATGTCGAAAAGCAATCCGTCTGAAGTGAAATTGGCCGCACCGACATc                                                                                     <  2:2152495/93‑1 (MQ=255)

       cTGACTCAGGAGCAGGTGATGGAAGCCGCATTGAGGAGCGAACATCATGTCGAAAAGCAATCCGTCTGAAGTGAAATTGGCCGCACCGACATc                                                                                     <  1:1660007/93‑1 (MQ=255)

        cgACTCCGGAGCAGGTGATGGAAGCCGCATTGAGGAGCGAACATCATGTCGAAAAGCACTCCGTCTGAAGTGAAATTGGCCGCACCGACATcc                                                                                    <  2:1905552/92‑1 (MQ=255)

         gACTCAGGAGCAGGTGATGGAAGCCGCATTGAGGAGCGAACATCATGTCGAAAAGCAATCCGTCTGAAGTGAAATTGGCCGCACCGACATCCg                                                                                   >  1:2496167/1‑93 (MQ=255)

         gACTCAGGAGCAGGTGATGGAAGCCGCATTGAGGAGCGAACATCATGTCGAAAAGCAATCCGTCTGAAGTGAAATTGGCCGCACCGACATCCg                                                                                   <  1:827444/93‑1 (MQ=255)

          aCTCAGGAGCAGGTGATGGAAGCCGCATTGAGGAGCGAACATCATGTCGAAAAGCAATCCGTCTGAAGTGAAATTGGCCGCACCGACATCCgg                                                                                  <  2:142341/93‑1 (MQ=255)

           cTCAGGAGCAGGTGATGGAAGCCGCATTGAGGAGCGAACATCATGTCGAAAAGCAATCCGTCTGAAGTGAAATTGGCCGCACCGACATCCggg                                                                                 >  2:1199763/1‑92 (MQ=255)

           cTCAGGAGCAGGTGATGGAAGCCGCATTGAGGAGCGAACATCATGTCGAAAAGCAATCCGTCTGAAGTGAAATTGGCCGCACCGACATCCGGt                                                                                 <  1:1778851/93‑1 (MQ=255)

           cTCAGGAGCAGGTGATGGAAGCCGCATTGAGGAGCGAACATCATGTCGAAAAGCAATCCGTCTGAAGTGAAATTGGCCGCACCGACATCCGGt                                                                                 <  2:3415304/93‑1 (MQ=255)

            tCAGGAGCAGGTGATGGAAGCCGCATTGAGGAGCGAACATCATGTCGAAAAGCAATCCGTCTGAAGTGAAATTGGCCGCACCGACATCCGGTg                                                                                <  1:4509182/93‑1 (MQ=255)

               ggAGCAGGTGATGGAAGCCGCATTGAGGAGCGAACATCATGTCGAAAAGCAATCCGTCTGAAGTGAAATTGGCCGCACCGACATCCGGTGGCt                                                                             >  2:4283573/1‑93 (MQ=255)

               ggAGCAGGTGATGGAAGCCGCATTGAGGAGCGAACATCATGACGAAAAGCAATCCTTTTTAAGTGAATTTGGCGGAACCGCATTCCGGTGACt                                                                             >  2:3306683/1‑93 (MQ=255)

               ggAGCAGGTGATGGAAGCCGCATTGAGAAGCGAACATCATGTCGAAAAGCAATCCGTCTGAAGTGAAATTGGCCGCACCGACATCCGGTGGCt                                                                             >  1:320068/1‑93 (MQ=255)

                 aGCAGGTGATGGAAGCCGCATTGAGGAGCGAACATCATGTCGAAAAGCAATCCGTCTGAAGTGAAATTGGCCGCACCGACATCCGGTGGCTtc                                                                           >  2:2512384/1‑93 (MQ=255)

                 aGCAGGTGATGGAAGCCGCATTGAGGAGCGAACATCATGTCGAAAAGCAATCCGTCTGAAGTGAAATTGGCCGCACCGACATCCGGTGGCTtc                                                                           <  1:537324/93‑1 (MQ=255)

                  gCAGGTGATGGAAGCCGCATTGAGGAGCGAACATCATGTCGAAAAGCAATCCGTCTGAAGTGAAATTGGCCGCACCGACATCCGGTGGCTtct                                                                          >  2:3387007/1‑93 (MQ=255)

                  gCAGGTGATGGAAGCCGCATGGAGGAGCGAACATCATGTCGAAAAGCAATCCGTCTGAAGTGAAATTGGCCGCACCGACATCCGGTGGCTtct                                                                          <  2:363445/93‑1 (MQ=255)

                   gaGGTGATGGAAGCCGCATTGAGGAGCGAACATCATGTCGAAAAGCAATCCGTCTGAAGTGAAATTGGCCGCACCGACATCCGGTGGCTtctc                                                                         <  2:1364593/92‑1 (MQ=255)

                   cAGGTGATGGAAGCCGCATTGAGGAGCGAACATCATGTCGAAAAGCAATCCGTCTGAAGTGAAATTGGCCGCACCGACATCCGGTGGCTtctc                                                                         <  1:4459239/93‑1 (MQ=255)

                   cAGGTGATGGAAGCCGCATTGAGGAGCGAACATCATGTCGAAAAGCAATCCGTCTGAAGTGAAATTGGCCGCACCGACATCCGGTGGCTtctc                                                                         <  1:1123284/93‑1 (MQ=255)

                    aGGTGATGGAAGCCGCATTGAGGAGCGAACATCATGTCGAAAAGCAATCCGTCTGAAGTGAAATTGGCCGCACCGACATCCGGTGGCTTCTcc                                                                        >  2:1213177/1‑93 (MQ=255)

                    aGGTGATGGAAGCCGCATTGAGGAGCGAACATCATGTCGAAAAGCAATCCGTCTGAAGTGAAATTGGCCGCACCGACATCCGGTGGCTTCTcc                                                                        <  1:393472/93‑1 (MQ=255)

                    aGGTGATGGAAGCCGCATTGAGGAGCGAACATCATGTCGAAAAGCAATCCGTCTGAAGTGAAATTGGCCGCACCGACATCCGGTGGCTTCTcc                                                                        >  2:2116271/1‑93 (MQ=255)

                     ggTGATGGAAGCCGCATTGAGGAGCGAACATCATGTCGAAAAGCAATCCGTCTGAAGTGAAATTGGGCGCACCGACATCCGGTGGCTTCTCCg                                                                       >  1:1968592/1‑93 (MQ=255)

                      gTGATGGAAGCCGCATTGAGGAGCGAACATCATGTCGAAAAGCAATCCGTCTGAAGTGAAATTGGCCGCACCGACATCCGGTGGCTTCTCCg‑g                                                                     <  2:3780442/93‑1 (MQ=255)

                       tGATGGAAGCCGCATTGAGGAGCGAACATCATGTCGAAAAGCAATCCGTCTGAAGTGAAATTGGCCGCACCGACATCCGGTGGCTTCTCCg‑gg                                                                    >  1:360521/1‑93 (MQ=255)

                        gATGGAAGCCGCATTGAGGAGCGAACATCATGTCGAAAAGCAATCCGTCTGAAGTGAAATTGGCCGCACCGACATCCGGTGGCTTCTCCG‑GGc                                                                   >  1:3038134/1‑93 (MQ=255)

                        gATGGAAGCCGCATTGAGGAGCGAACATCATGTCGAAAAGCAATCCGTCTGAAGTGAAATTGGCCGCACCGACATCCGGTGGCTTCTCCG‑GGc                                                                   >  1:2422924/1‑93 (MQ=255)

                        gATGGAAGCCGCATTGAGGAGCGAACATCATGTCGAAAAGCAATCCGTCTGAAGTGAAATTGGCCGCACCGACATCCGGTGGCTTCTCCG‑GGc                                                                   <  2:3605712/93‑1 (MQ=255)

                        gATGGAAGCCGCATTGAGGAGCGAACATCATGTCGAAAAGCAATCCGTCTGAAGTGAAATTGGCCGCACCGACATCCGGTGGCTTCTCCG‑GGc                                                                   >  1:3936193/1‑93 (MQ=255)

                         aTGGAAGCCGCATTGAGGAGCGAACATCATGTCGAAAAGCAATCCGTCTGAAGTGAAATTGGCCGCACCGACATCCGGTGGTTTCTCCG‑GGCt                                                                  <  1:506794/93‑1 (MQ=255)

                          tGGAAGCCGCATTGAGGAGCGAACATCATGTCGAAAAGCAATCCGTCTGAAGTGAAATTGGCCGCACCGACATCCGGTGGCTTCTCCG‑GGCTg                                                                 >  2:467570/1‑93 (MQ=255)

                               gCCGCATTGAGGAGCGAACATCATGTCGAAAAGCAATCCGTCTGAAGTGAAATTGGCCGCACCGACATCCGGTGGCTTCTCCG‑GGCTGAAATc                                                            >  2:2680552/1‑93 (MQ=255)

                                  gCATTGAGGAGCGAACATCATGTCGAAAAGCAATCCGTCTGAAGTGAAATTGGCCGCACCGACATCCGGTGGCTTCTCCG‑GGCTGAAATCACt                                                         >  2:4143122/1‑93 (MQ=255)

                                       gaggagCGAACATCATGTCGAAAAGCAATCCGTCTGAAGTGAAATTGGCCGCACCGACATCCGGTGGCTTCTCCG‑GGCTGAAATCACTGAAtt                                                    >  1:4234310/1‑93 (MQ=255)

                                       gaggagCGAACATCATGTCGAAAAGCAATCCGTCTGAAGTGAAATTGGCCGCACCGACATCCGGTGGCTTCTCCG‑GGCTGAAATCACTGAAtt                                                    <  1:43786/93‑1 (MQ=255)

                                         ggagCGAACATCATGTCGAAAAGCAATCCGTCTGAAGTGAAATTGGCCGCACCGACATCCGGTGGCTTCTCCG‑GGCTGAAATCACTGAATTTg                                                  <  2:2518202/93‑1 (MQ=255)

                                         ggagCGAACATCATGTCGAAAAGCAATCCGTCTGAAGTGAAATTGGCCGCACCGACATCCGGTGGCTTCTCCG‑GGCTGAAATCACTGAATTTg                                                  <  1:1726860/93‑1 (MQ=255)

                                          gagCGAACATCATGTCGAAAAGCCATCCGTCTGAAGTGAAATTGGCCGCACCGACATCCGGTGGCTTCTCCG‑GGCTGAAATCACTGAATTTGc                                                 >  2:2434281/1‑93 (MQ=255)

                                           agCGAACATCATGTCGAAAAGCAATCCGTCTGAAGTGAAATTGGCCGCACCGACATCCGGTGGCTTCTCCG‑GGCTGAAATCACTGAATTTGCa                                                <  1:404575/93‑1 (MQ=255)

                                           agCGAACATCATGTCGAAAAGCAATCCGTCTGAAGTGAAATTGGCCGCACCGACATCCGGTGCCTTCTCCG‑GGCTGAAATCACTGAATTTGCa                                                <  1:404576/93‑1 (MQ=255)

                                            gCGAACATCATGTCGAAAAGCAATCCGTCTGAAGTGAAATTGGCCGCACCGACATCCGGTGGCTTCTCCG‑GGCTGAAATCACTGAATTTGCAg                                               >  1:905291/1‑93 (MQ=255)

                                            gCGAACATCATGTCGAAAAGCAATCCGTCTGAAGTGAAATTGGCCGCACCGACATCCGGTGGCTTCTCCG‑GGCTGAAATCACTGAATTTGCAg                                               >  2:1049867/1‑93 (MQ=255)

                                            gCGAACATCATGTCGAAAAGCAATCCGGCTGAAGTGAAATTGGCCGCACCGACATCCGGTGGCTTCTCCG‑GGCTGAAATCACTGAATTTGCAg                                               <  1:867343/93‑1 (MQ=255)

                                             cGAACATCATGTCGAAAAGCAATCCGTCTGAAGTGAAATTGGCCGCACCGACATCCGGTGGCTTCTCCG‑GGCTGAAATCACTGAATTTGCAgg                                              >  2:3808678/1‑93 (MQ=255)

                                              gAACATCATGTCGAAAAGCAATCCGTCTGAAGTGAAATTGGCCGCACCGACATCCGGTGGCTTCTCCG‑GGCTGAAATCACTGAATTTGCAGGt                                             >  2:698632/1‑93 (MQ=255)

                                              gAACATCATGTCGAAAAGCAATCCGTCTGAAGTGAAATTGGCCGCACCGACATCCGGTGGCTTCTCCG‑GGCTGAAATCACTGAATTTGCAGGt                                             <  1:552741/93‑1 (MQ=255)

                                                aCATCATGTCGAAAAGCAATCCGTCTGAAGTGAAATTGGCCGCACCGACATCCGGTGGCTTCTCCG‑GGCTGAAATCACTGAATTTGCAGGTCt                                           <  2:1915729/93‑1 (MQ=255)

                                                  atcatGTCGAAAAGCAATCCGTCTGAAGTGAAATTGGCCGCACCGACATCCGGTGGCTTCTCCG‑GGCTGAAATCACTGAATTTGCAGGTCTTc                                         >  2:282242/1‑93 (MQ=255)

                                                  atcatGTCGAAAAGCAATCCGTCTGAAGTGAAATTGGCCGCACCGACATCCGGTGGCTTCTCCG‑GGCTGAAATCACTGAATTTGCAGGTCTTc                                         <  2:540038/93‑1 (MQ=255)

                                                   tcatGTCGAAAAGCAATCCGTCTGAAGTGAAATTGGCCGCACCGACATCCGGTTGCTTCTCCG‑GGCTGAAATCACTGAATTTGCAGGTCTTCg                                        >  2:3626571/1‑93 (MQ=255)

                                                   tcatGTCGAAAAGCAATCCGTCTGAAGTGAAATTGGCCGCACCGACATCCGGTGGCTTCTCCG‑GGCTGAAATCACTGAATTTGCAGGTCTTCg                                        <  2:2448272/93‑1 (MQ=255)

                                                   tcatGTAAAAAAGCAATCCGTCTGAAGTGAAATTGCCCGCACCGACATCCGGTGGCTTCTCCG‑GGCTGAAATCACTGAATTTGCAGGTCTTCg                                        <  1:880428/93‑1 (MQ=255)

                                                    catGTCGAAAAGCAATCCGTCTGAAGTGAAATTGGCCGCACCGACATCCGGTGGCTTCTCCG‑GGCTGAAATCACTGAATTTGCAGGTCTTCGt                                       <  2:1007069/93‑1 (MQ=255)

                                                     atGTCGAAAAGCAATCCGTCTGAAGTGAAATTGGCCGCACCGACATCCGGTGGCTTCTCCG‑GGCTGAAATCACTGAATTTGCAGGTCTTCGtg                                      <  1:3415012/93‑1 (MQ=255)

                                                     atGTCGAAAAGCAATCCGTCTGAAGTGAAATTGGCCGCACCGACATCCGGTGGCTTCTCCG‑GGCTGAAATCACTGAATTTGCAGGTCTTCGtg                                      >  1:3817565/1‑93 (MQ=255)

                                                      tGTCGAAAAGCAATCCGTCTGAAGTGAAATTGGCCGCACCGACATCCGGTGGCTTCTCCG‑GGCTGAAATCACTGAATTTGCAGGTCTTCGtga                                     >  2:2860264/1‑93 (MQ=255)

                                                      tGTCGAAAAGCAATCCGTCTGAAGTGAAATTGGCCGCACCGACATCCGGTGGCTTCTCCG‑GGCTGAAATCACTGAATTTGCAGGTCTTCGtga                                     <  1:2189530/93‑1 (MQ=255)

                                                       gTCGAAAAGCAATCCGTCTGAAGTGAAATTGGCCGCACCGACATCCGGTGGCTTCTCCGAGGCTG‑AATCACTGAATTTGCAGGTCTTCGtgat                                    >  2:4386855/1‑93 (MQ=255)

                                                            aaaGCAATCCGTCTGAAGTGAAATTGGCCGCACCGACATCCGGTGGCTTCTCCG‑GGCTGAAATCACTGAATTTGCAGGTCTTCGTGATGATTg                               >  1:846022/1‑93 (MQ=255)

                                                            aaaGCAATCCGTCTGAAGTGAAATTGGCCGCACCGACATCCGGTGGCTTCTCCG‑GGCTGAAATCACTGAATTTGCAGGTCTTCGTGATGATTg                               <  1:2045202/93‑1 (MQ=255)

                                                             aaGCAATCCGTCTGAAGTGAAATTGGCCGCACCGACATCCGGTGGCTTCTCCG‑GGCTGAAATCACTGAATTTGCAGGTCTTCGTGATGATTGc                              >  2:1739697/1‑93 (MQ=255)

                                                             aaGCAATCCGTCTGAAGTGAAATTGGCCGCACCGACATCCGGTGGCTTCTCCG‑GGCTGAAATCACTGAATTTGCAGGTCTTCGTGATGATTGc                              >  1:3112521/1‑93 (MQ=255)

                                                                cAATCCGTCTGAAGTGAAATTGGCCGCACCGACATCCGGTGGCTTCTCCG‑GGCTGAAATCACTGAATTTGCAGGTCTTCGTGATGATTGCAGc                           <  1:258316/93‑1 (MQ=255)

                                                                cAATCCGTCTGAAGTGAAATTGGCCGCACCGACATCCGGTGGCTTCTCCG‑GGCTGAAATCACTGAATTTGCAGGTCTTCGTGATGATTGCAGc                           >  1:2846760/1‑93 (MQ=255)

                                                                    ccGTCTGAAGTGAAATTGGCCGCACCGACATCCGGTGGCTTCTCCG‑GGCTGAAATCACTGAATTTGCAGGTCTTCGTGATGATTGCAGCTatc                       <  2:3066401/93‑1 (MQ=255)

                                                                     cGTCTGAAGTGAAATTGGCCGCACCGACATCCGGTGGCTTCTCCG‑GGCTGAAATCACTGAATTTGCAGGTCTTCGTGATGATTGCAGCTatca                      <  2:2775374/93‑1 (MQ=255)

                                                                       tCTGAAGTGAAATTGGCCGCACCGACATCCGGTGGCTTCTCCG‑GGCTGAAATCACTGAATTTGCAGGTCTTCGTGATGATTGCAGCTatcatc                    <  1:214124/93‑1 (MQ=255)

                                                                       tCTGAAGTGAAATTGGCCGCACCGACATCCGGTGGCTTCTCCG‑GGCTGAAATCACTGAATTTGCAGGTCTTCGTGATGATTGCAGCTatcatc                    <  2:4545550/93‑1 (MQ=255)

                                                                       tCTGAAGTGAAATTGGCCGCACCGACATCCGGTGGCTTCTCCG‑GGCTGAAATCACTGAATTTGCAGGTCTTCGTGATGATTGCAGCTatcatc                    <  1:580943/93‑1 (MQ=255)

                                                                       tCTGAAGTGAAATTGGCCGCACCGACATCCGGTGGCTTCTCCG‑GGCTGAAATCACTGAATTTGCAGGTCTTCGTGATGATTGCAGCTatcatc                    <  1:4700097/93‑1 (MQ=255)

                                                                         cgAAGTGAAATTGGCCGTATCGACATCCGGTGGCTTCTCCG‑GGCTGAAATCACTGAATTTGCAGGTCTTCGTGATGATTGCAGCTATCATCGc                  <  1:2689912/92‑1 (MQ=255)

                                                                           aaGTGAAATTGGCCGCACCGACATCCGGTGGCTTCTCCG‑GGCTGAAATCACTGAATTTGCAGGTCTTCGTGATGATTGCAGCTATCATCGCaa                <  1:1480059/93‑1 (MQ=255)

                                                                           aaGTGAAATTGGCCGCACCGACATCCGGTGGCTTCTCCG‑GGCTGAAATCACTGAATTTGCAGGTCTTCGTGATGATTGCAGCTATCATCGCaa                >  1:2051859/1‑93 (MQ=255)

                                                                            aGTGAAATTGGCCGCACCGACATCCGGTGGCTTCTCCG‑GGCTGAAATCACTGAATTTGCAGGTCTTCGTGATGATTGCAGCTATCATCGCaac               >  2:3933743/1‑92 (MQ=255)

                                                                            aGTGAAATTGGCCGCACCGACATCCGGTGGCTTCTCCG‑GGCTGAAATCACTGAATTTGCAGGTCTTCGTGATGATTGCAGCTATCATCGCAAt               >  1:1762262/1‑93 (MQ=255)

                                                                             gTGAAATTGGCCGCACCGACATCCGGTGGCTTCTCCG‑GGCTGAAATCACTGAATTTGCAGGTCTTCGTGATGATTGCAGCTATCATCGCAATc              >  1:3317173/1‑93 (MQ=255)

                                                                             gTGAAATTGGCCGCACCGACATCCGGTGGCTTCTCCG‑GGCTGAAATCACTGAATTTGCAGGTCTTCGTGATGATTGCAGCTATCATCGCAATc              >  2:157992/1‑93 (MQ=255)

                                                                             gTGAAATTGGCCGCACCGACATCCGGCGGCTTCTCCG‑GGCTGAAATCACTGAATTTGCAGGTCTTCGTGATGATTGCAGCTATCATCGCAATc              <  1:3256935/93‑1 (MQ=255)

                                                                              tGAAATTGGCCGCACCGACATCCGGTGGCTTCTCCG‑GGCTGAAATCACTGAATTTGCAGGTCTTCGTGATGATTGCAGCTATCATCGCAATCa             <  1:961328/93‑1 (MQ=255)

                                                                               gAAATTGGCCGCACCGACATCCGGTGGCTTCTCCG‑GGCTGAAATCACTGAATTTGCAGGTCTTCGTGATGATTGCAGCTATCATCGCAATCAt            <  2:4160490/93‑1 (MQ=255)

                                                                               gAAATTGGCCGCACCGACATCCGGTGGCTTCTCCG‑GGCTGAAATCACTGAATTTGCAGGTCTTCGTGATGATTGCAGCTATCATCGCAATCAt            >  2:4444585/1‑93 (MQ=255)

                                                                                aaaTTGGCCGCACCGACATCCGGTGGCTTCTCCG‑GGCTGAAATCACTGAATTTGCAGGTCTTCGTGATGATTGCAGCTATCATCGCAATCATg           <  1:133784/93‑1 (MQ=255)

                                                                                aaaTTGGCCGCACCGACATCCGGTGGCTTCTCCG‑GGCTGAAATCACTGAATTTGCAGGTCTTCGTGATGATTGCAGCTATCATCGCAATCATg           >  2:315739/1‑93 (MQ=255)

                                                                                 aaTTGGCCGCACCGACATCCGGTGGCTTCTCCG‑GGCTGAAATCACTGAATTTGCAGGTCTTCGTGATGATTGCAGCTATCATCGCAATCATGc          <  1:4193107/93‑1 (MQ=255)

                                                                                 aaTTGGCCGCACCGACATCCGGTGGCTTCTCCG‑GGCTGAAATCACTGAATTTGCAGGTCTTCGTGATGATTGCAGCTATCATCGCAATCATGc          <  1:3089013/93‑1 (MQ=255)

                                                                                  aTTGGCCGCACCGACATCCGGTGGCTTCTCCG‑GGCTGAAATCACTGAATTTGCAGGTCTTCGTGATGATTGCAGCTATCATCGCAATCATGCt         >  1:1041222/1‑93 (MQ=255)

                                                                                   ttGGCCGCACCGACATCCGGTGGCTTCTCCG‑GGCTGAAATCACTGAATTTGCAGGTCTTCGTGATGATTGCAGCTATCATCGCAATCATGCTg        >  2:2714026/1‑93 (MQ=255)

                                                                                    tGGCCGCACCGACATCCGGTGGCTTCTCCG‑GGCTGAAATCACTGAATTTGCAGGTCTTCGTGATGATTGCAGCTATCATCGCAATCATGCTGt       >  2:1226213/1‑93 (MQ=255)

                                                                                      gCCGCACCGACATCCGGTGGCTTCTCCG‑GGCTGAAATCACTGAATTTGCAGGTCTTCGTGATGATTGCAGCTATCATCGCAATCATGCTGTTc     >  2:1487741/1‑93 (MQ=255)

                                                                                      gCCGCACCGACATCCGGTGGCTTCTCCG‑GGCTGAAATCACTGAATTTGCAGGTCTTCGTGATGATTGCAGCTATCATCGCAATCATGCTGTTc     <  2:4727230/93‑1 (MQ=255)

                                                                                       ccGCACCGACATCCGGTGGCTTCTCCG‑GGCTGAAATCACTGAATTTGCAGGTCTTCGTGATGATTGCAGCTATCATCGCAATCATGCTGTTCt    <  1:4282765/93‑1 (MQ=255)

                                                                                       ccGCACCGACATCCGGTGGCTTCTCCG‑GGCTGAAATCACTGAATTTGCAGGTCTTCGTGATGATTGCAGCTATCATCGCAATCATGCTGTTCt    <  1:1205111/93‑1 (MQ=255)

                                                                                         ctACCGGCACCCGGAGGCTTCTCTG‑GGCTGAAATCACTGTAATTGTAGGTCTTCGTGATGATTGCTGCTATGATCGCGATCCTGCTGTTCttt  >  2:3669324/2‑93 (MQ=255)

TCATAACCTGACTCAGGAGCAGGTGATGGAAGCCGCATTGAGGAGCGAACATCATGTCGAAAAGCAATCCGTCTGAAGTGAAATTGGCCGTACCGACATCCGGTGGCTTCTCCG‑GGCTGAAATCACTGAATTTGCAGGTCTTCGTGATGATTGCAGCTATCATCGCAATCATGCTGTTCTTT  >  NC_000913/3731296‑3731477

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`