BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,744,796	G→A	G28D (GGC→GAC)	lyxK →	L‑xylulose kinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,744,796	0	G	A	99.2%	427.4 / NA	126	G28D (GGC→GAC)	lyxK	L‑xylulose kinase
Reads supporting (aligned to +/- strand): ref base G (1/0); major base A (62/62); minor base C (0/1); total (63/63)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 140 total aligned reads displayed.

AATAAACCATGACGCAATACTGGCTGGGGTTAGATTGTGGCGGTAGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGGCGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCGCGATATGGCAGAACTGTGGCAATGCTGCATGGCTG  >  NC_000913/3744706‑3744885

aaTAAACCATGACGCAATACTGGCTGGGGTTAGATTGTGGCGGTAGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGACg                                                                                         >  2:4578112/1‑93 (MQ=255)

aaTAAACCATGACGCAATACTGGCTGGGGTTAGATTGTGGCGGTAGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGACg                                                                                         >  1:691169/1‑93 (MQ=255)

   aaaCCATGACGCAATACTGGCTGGGGTTAGATTGTGGCGGTAGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGACGTGc                                                                                      >  1:1963703/1‑93 (MQ=255)

     aCCATGACGCAATACTGGCTGGGGTTAGATTGTGGCGGTAGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGACGTGCAg                                                                                    <  1:4328272/93‑1 (MQ=255)

      ccATGACGCAATACTGGCTGGGGTTAGATTGTGGCGGTAGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGACGTGCAgc                                                                                   >  1:3915121/1‑93 (MQ=255)

      ccATGACGCAATACTGGCTGGGGTTAGATTGTGGCGGTAGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGACGTGCAgc                                                                                   >  2:3378047/1‑93 (MQ=255)

       cATGACTCAATACTGGCTGGAGTTAGATTGTGGCGGTAGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGACGTGCAgcg                                                                                  <  2:768733/93‑1 (MQ=255)

        aTGACGCAATACTGGCTGGGGTTAGATTGTGGCGGTAGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGACGTGCAgcgc                                                                                 >  1:2258450/1‑93 (MQ=255)

        aTGACGCAATACTGGCTGGGGTTAGATTGTGGCGGTAGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGACGTGCAgcgc                                                                                 >  2:1895602/1‑93 (MQ=255)

         tGACGCAATACTGGCTGGGGTTAGATTGTGGCGGTAGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGACGTGCAGCGcc                                                                                <  1:2580587/93‑1 (MQ=255)

          gACGCAATACTGGCTGGGGTTAGATTGTGGCGGTAGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGACGTGCAGCGCCt                                                                               <  1:533016/93‑1 (MQ=255)

          gACGCAATACTGGCTGGGGTTAGATTGTGGCGGTAGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGACGTGCAGCGCCt                                                                               <  2:1716483/93‑1 (MQ=255)

          gACGCAATACTGGCTGGGGTTAGATTGTGGCGGTAGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGACGTGCAGCGCCt                                                                               <  1:641666/93‑1 (MQ=255)

             gCAATACTGGCTGGGGTTAGATTGTGGCGGTAGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGACGTGCAGCGCCTGcc                                                                            <  1:3141317/93‑1 (MQ=255)

              cAATACTGGCTGGGGTTAGATTGTGGCGGTAGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGACGTGCAGCGCCTGCCg                                                                           >  1:2057019/1‑93 (MQ=255)

                 tACTGGCTGGGGTTAGATTGTGGCGGTAGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGACGTGCAGCGCCTGCCGCtg                                                                        <  1:2932701/93‑1 (MQ=255)

                 tACTGGCTGGGGTTAGATTGTGGCGGTAGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGACGCAGACGTGCAGCGCCTGCCGCtg                                                                        <  2:1015059/93‑1 (MQ=255)

                  aCTGGCTGGGGTTAGATTGTGGCGGTAGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGACGTGCAGCGCCTGCCGCtgt                                                                       <  1:2339471/93‑1 (MQ=255)

                    tggctggGGTTAGATTGTGGCGGTAGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGACGTGCAGCGCCTGCCGCTGTgc                                                                     <  2:2514721/93‑1 (MQ=255)

                      gctggGGTTAGATTGTGGCGGTAGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGACGTGCAGCGCCTGCCGCTGTgcgc                                                                   >  2:3442134/1‑93 (MQ=255)

                      gctggGGTTAGATTGTGGCGGTAGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGACGTGCAGCGCCTGCCGCTGTgcgc                                                                   >  2:32667/1‑93 (MQ=255)

                      gctggGGTTAGATTGTGGCGGTAGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGACGTGCAGCGCCTGCCGCTGTgcgc                                                                   >  1:4019139/1‑93 (MQ=255)

                         ggggTTAGATTGTGGCGGTAGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGACGTGCAGCGCCTGCCGCTGTGCGCAtt                                                                <  2:3556245/93‑1 (MQ=255)

                         ggggTTAGATTGTGGCGGTAGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGACGTGCAGCGCCTGCCGCTGTGCGCAtt                                                                >  1:1872172/1‑93 (MQ=255)

                         ggggTTAGATTGTGGCGGTAGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGACGTGCAGCGCCTGCCGCTGTGCGCAtt                                                                >  2:2891827/1‑93 (MQ=255)

                           ggTTAGATTGGGGCGGTAGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGACGTGCAGCGCCTGCCGCTGTGCGCATTaa                                                              <  1:1188043/93‑1 (MQ=255)

                             ttAGATTGTGGCGGTAGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGACGTGCAGCGCCTGCCGCTGTGCGCATTAAGc                                                            >  2:3088984/1‑93 (MQ=255)

                              tAGATTGTGGCGGTAGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGACGTGCAGCGCCTGCCGCTGTGCGCATTAAGcc                                                           <  1:3820985/93‑1 (MQ=255)

                               aGATTGTGGCGGTAGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGACGTGCAGCGCCTGCCGCTGTGCGCATTAAGccc                                                          >  1:4470174/1‑93 (MQ=255)

                               aGATTGTGGCGGTAGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGACGTGCAGCGCCTGCCGCTGTGCGCATTAAGccc                                                          <  1:1091080/93‑1 (MQ=255)

                               aGATTGTGGCGGTAGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGACGTGCAGCGCCTGCCGCTGTGCGCATTAAGccc                                                          >  1:1969831/1‑93 (MQ=255)

                                 aTTGTGGCGGTAGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGACGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGc                                                        >  2:102382/1‑93 (MQ=255)

                                  ttGTGGCGGTAGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGACGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCa                                                       >  2:157465/1‑93 (MQ=255)

                                  ttGTGGCGGTAGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGACGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCa                                                       <  2:602976/93‑1 (MQ=255)

                                  ttGTGGCGGTAGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGACGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCa                                                       <  1:1347081/93‑1 (MQ=255)

                                     tgGCGGTAGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGACGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGcc                                                    <  1:267033/93‑1 (MQ=255)

                                     gggCGGTAGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGACGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGcc                                                    <  2:1295149/92‑1 (MQ=255)

                                      ggCGGTCGCTGGCTGCCGGCCGGGCTGTGTGCCCGCGAGGGCCGGGGGGCAGCCGTGCGGCGCCTGCCGCTGTGCGCATTAAGCCCGCCGCCa                                                   <  1:3600691/93‑1 (MQ=255)

                                      ggCGGTAGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGACGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCa                                                   <  2:474107/93‑1 (MQ=255)

                                      ggCGGTAGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGACGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCa                                                   <  1:259100/93‑1 (MQ=255)

                                           tAGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGACGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTg                                              <  2:738702/93‑1 (MQ=255)

                                            agatggctgAAAGCGGGGCTGTAGTACCGCGAAGGCCGGGGGGCAGACGTGCAGCGCCTGCCGCTGTGCTCATTAAGCCCGCAGCCAGGCTgg                                             <  2:1162340/90‑1 (MQ=255)

                                            aGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGACGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTgg                                             >  1:3767272/1‑93 (MQ=255)

                                            aGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGACGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTgg                                             <  2:1162341/93‑1 (MQ=255)

                                              ctggctgAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGACGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGc                                           <  1:1067750/93‑1 (MQ=255)

                                               tggctgAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGGCGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCa                                          >  1:1970733/1‑93 (MQ=255)

                                               tggctgAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGACGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCa                                          >  2:4153147/1‑93 (MQ=255)

                                               tggctgAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGACGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCa                                          >  2:3536893/1‑93 (MQ=255)

                                                ggctgAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGACGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCag                                         >  2:411531/1‑93 (MQ=255)

                                                ggctgAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGACGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCag                                         >  2:1764086/1‑93 (MQ=255)

                                                ggctgAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGACGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCag                                         >  1:1287866/1‑93 (MQ=255)

                                                ggctgAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGACGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCag                                         >  1:1093796/1‑93 (MQ=255)

                                                ggctgAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGACGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCag                                         >  2:2758629/1‑93 (MQ=255)

                                                 gctgAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGACGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGGagg                                        >  2:2852887/1‑92 (MQ=255)

                                                 gctgAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGACGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCaga                                        <  2:122823/93‑1 (MQ=255)

                                                 gctgAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGACGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCaga                                        <  2:546468/93‑1 (MQ=255)

                                                 gctgAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGACGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCaga                                        <  1:1466698/93‑1 (MQ=255)

                                                 gctgAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGACGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCaga                                        <  1:2388622/93‑1 (MQ=255)

                                                  ctgAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGACGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCagag                                       <  2:3361591/93‑1 (MQ=255)

                                                  ctgAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGACGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCagag                                       <  2:4328196/93‑1 (MQ=255)

                                                   tgAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGACGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCacagg                                      >  1:1608363/1‑92 (MQ=255)

                                                    gAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGACGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGGCGAgcg                                     >  2:1609738/1‑93 (MQ=255)

                                                      aaGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGACGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGcgcg                                   >  1:2103219/1‑93 (MQ=255)

                                                      aaGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGACGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGcgcg                                   >  1:1892089/1‑93 (MQ=255)

                                                       aGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGACGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCGCGa                                  <  2:2196547/93‑1 (MQ=255)

                                                       aGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGACGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCGCGa                                  <  1:3509678/93‑1 (MQ=255)

                                                        gCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGACGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCGCGat                                 >  1:1528877/1‑93 (MQ=255)

                                                          cGGGCTGTATGACCGCGAAGGCCGGGAGGCAGACGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCGCGatat                               >  2:136754/1‑93 (MQ=255)

                                                          cGGGCTGTATGACCGCGAAGGCCGGGAGGCAGACGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCGCGatat                               <  2:569760/93‑1 (MQ=255)

                                                           gggCTGTATTGCCGCGAAGGCCGGGAGGCAGACGGGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCGCGATATg                              <  1:3662287/93‑1 (MQ=255)

                                                           gggCTGTATGACCGCGAAGGCCGGGAGGCAGACGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCGCGATATg                              >  2:823370/1‑93 (MQ=255)

                                                            ggCTGTATGACCGCGAAGGCCGGGAGGCAGACGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCGCGATATgg                             >  2:24785/1‑93 (MQ=255)

                                                            ggCTGTATGACCGCAAAGGCCGGGAGGCAGACGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCGCGATATgg                             <  2:494303/93‑1 (MQ=255)

                                                            ggCGGTATGACCGCGAAGGCCGGGAGGCAGACGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCGCGATATgg                             <  1:4398972/93‑1 (MQ=255)

                                                             gCTGTATGACCGCGAAGGCCGGGAGGCAGACGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCGCGATATGGc                            <  2:3059121/93‑1 (MQ=255)

                                                               tGTATGACCGCGAAGGCCGGGAGGCAGACGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCGCGATATGGCAg                          >  2:3647975/1‑93 (MQ=255)

                                                                     aCCGCGAAGGCCGGGAGGCAGACGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCGCGATATGGCAGAACtgt                    >  1:3004455/1‑93 (MQ=255)

                                                                     aCCGCGAAGGCCGGGAGGCAGACGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCGCGATATGGCAGAACtgt                    >  1:4338949/1‑93 (MQ=255)

                                                                      ccGCGAAGGCCGGGAGGCAGACGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCGCGATATGGCAGAACtgtg                   <  1:3180799/93‑1 (MQ=255)

                                                                      ccGCGAAGGCCGGGAGGCAGACGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCGCGATATGGCAGAACtgtg                   <  1:2833876/93‑1 (MQ=255)

                                                                        gcgAAGGCCGGGAGGCAGACGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCGCGATATGGCAGAACTGTGGc                 >  2:47651/1‑93 (MQ=255)

                                                                          gAAGGCGGGGAGGCAGACGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCGCGATATGGCAGAACTGTGGCaa               <  1:1774664/93‑1 (MQ=255)

                                                                          gAAGGCCGGGAGGCAGACGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCGCGATATGGCAGAACTGTGGGaa               >  1:2836343/1‑93 (MQ=255)

                                                                           aaGGCCGGGAGGCAGACGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCGCGATATGGCAGAACTGTGGCAAt              <  2:4494578/93‑1 (MQ=255)

                                                                           aaGGCCGGGAGGCAGACGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCGCGATATGGCAGAACTGTGGCAAt              <  1:3949797/93‑1 (MQ=255)

                                                                           aaGGCCGGGAGGCAGACGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCGCGATATGGCAGAACTGTGGCAAt              >  1:3415333/1‑93 (MQ=255)

                                                                            aGGCCGGGAGGCAGACGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCGCGATATGGCAGAACTGTGGCAAtg             <  2:2223122/93‑1 (MQ=255)

                                                                              gCCGGGAGGCAGACGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCGCGATATGGCAGAACTGTGGCAAtgct           <  2:633426/93‑1 (MQ=255)

                                                                                 gggAGGCAGACGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCGCGATATGGCAGAACTGTGGCAATGCTGCa        <  1:55899/93‑1 (MQ=255)

                                                                                 gggAGGCAGACGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCGCGATATGGCAGAACTGTGGCAATGCTGCa        >  1:895481/1‑93 (MQ=255)

                                                                                 gggAGGCAGACGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCGCGATATGGCAGAACTGTGGCAATGCTGCa        >  2:1014282/1‑93 (MQ=255)

                                                                                 gggAGGCAGACGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCGCGATATGGCAGAACTGTGGCAATGCTGCa        >  1:3017587/1‑93 (MQ=255)

                                                                                  ggAGGCAGGCGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCGCGATATGGCAGAACTGTGGCAATGCTGCAt       <  1:4039891/93‑1 (MQ=255)

                                                                                    aggcagacGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCGCGATATTGCAGAACTGTGGCAATGCTGCATgg     >  2:3656203/1‑93 (MQ=255)

                                                                                     ggcaggcGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCGCGATATGGCAGGACTGTGGCAATGCTGCATggt    >  2:4257947/1‑92 (MQ=255)

                                                                                     ggcagacGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCGCGATATGGCAGAACTGTGGCAATGCTGCATGGc    <  2:4559252/93‑1 (MQ=255)

                                                                                     ggcagacGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCGCGATATGGCAGAACTGTGGCAATGCTGCATGGc    <  2:2345067/93‑1 (MQ=255)

                                                                                      gcagacGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCGCGATATGGCAGAACTGTGGCAATGCTGCATGGCt   <  2:2929775/93‑1 (MQ=255)

                                                                                       cagacGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCGCGATATGGCAGAACTGTGGCAATGCTGCATGGCTg  <  1:4450460/93‑1 (MQ=255)

                                                                                       cagacGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCGCGATATGGCAGAACTGTGGCAATGCTGCATGGCTg  >  2:2217442/1‑93 (MQ=255)

AATAAACCATGACGCAATACTGGCTGGGGTTAGATTGTGGCGGTAGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGGCGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCGCGATATGGCAGAACTGTGGCAATGCTGCATGGCTG  >  NC_000913/3744706‑3744885

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 18 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`