BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,754,310	C→A	R382M (AGG→ATG)	yiaY ←	putative oxidoreductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,754,310	0	C	A	100.0%	837.5 / NA	233	R382M (AGG→ATG)	yiaY	putative oxidoreductase
Reads supporting (aligned to +/- strand): ref base C (0/0); new base A (115/118); total (115/118)

Only 100 of 238 total aligned reads displayed.

GACAGCTATCACGAATTTACGGGCAAGGGTTACAGAGATAGTTGAAAAAGCGGCTAACAATTTGCCAGCCGTTGTGGAAATGATGATTACCTCGCTGCATAAATCGCCACAATTTCTTCGTGAGTTGCCTGGATCGGGTTAGTAAAGCCACAGGCATCTTTCAGGGCATTAGTCGCCAA  >  NC_000913/3754220‑3754398

gACAGCTATCACGAATTTACGGGCAAGGGTTACAGAGATAGTTGAAAAAGCGGCTAACAATTTGCCAGCCGTTGTGGAAATGATGATTACATc                                                                                        >  2:329804/1‑93 (MQ=255)

gACAGCTATCACGAATTTACGGGCAAGGGTTACAGAGATAGTTGAAAAAGCGGCTAACAATTTGCCAGCCGTTGTGGAAATGATGATTACATc                                                                                        >  1:4138678/1‑93 (MQ=255)

 aCAGCTATCACGAATTTACGGGCAAGGGTTACAGAGATAGTTGAAAAAGCGGCTAACAATTTGCCAGCCGTTGTGGAAATGATGATTACATCg                                                                                       >  2:2932689/1‑93 (MQ=255)

  cAGCTATCACGAATTTACGGGCAAGGGTTACAGAGATAGTTGAAAAAGCGGCTAACAATTTGCCAGCCGTTGTGGAAATGATGATTACATCGc                                                                                      <  1:2283068/93‑1 (MQ=255)

   aGCTATCACGAATTTACGGGCAAGGGTTACAGAGATAGTTGAAAAAGCGGCTAACAATTTGCCAGCCGTTGTGGAAATGATGATTACATCGCt                                                                                     <  1:1964504/93‑1 (MQ=255)

    gCTATCACGAATTTACGGGCAAGGGTTACAGAGATAGTTGAAAAAGCGGCTAACAATTTGCCAGCCGTTGTGGAAATGATGATTACATCGCTg                                                                                    >  2:513473/1‑93 (MQ=255)

    gCTATCACGAATTTACGGGCAAGGGTTACAGAGATAGTTGAAAAAGCGGCTAACAATTTGCCAGCCGTTGTGGAAATGATGATTACATCGCTg                                                                                    >  1:4584662/1‑93 (MQ=255)

    gCTATCACGAATTTACGGGCAAGGGTTACAGAGATAGTTGAAAAAGCGGCTAACAATTTGCCAGCCGTTGTGGAAATGATGATTACATCGCTg                                                                                    >  2:1173095/1‑93 (MQ=255)

    gCTATCACGAATTTACGGGCAAGGGCTACAGAGATAGTTGAAAAAGCGGCTAACAATTTGCCAGCCGTTGTGGAAATGATGATTACATCGCtc                                                                                    >  2:3173611/1‑92 (MQ=255)

      tATCACGAATTTACGGGCAAGGGTTACAGAGATAGTTGAAAAAGCGGCTAACAATTTGCCAGCCGTTGTGGAAATGATGATTACATCGCTGCg                                                                                  <  1:3210233‑M1/93‑2 (MQ=255)

       aTCACGAATTTACGGGCAAGGGTTACAGAGATAGTTGAAAAAGCGGCTAACAATTTGCCAGCCGTTGTGGAAATGATGATTACATCGCTGCgc                                                                                 <  1:671217‑M1/93‑3 (MQ=255)

          aCGAATTTACGGGCAAGGGTTACAGAGATAGTTGAAAAAGCGGCTAACAATTTGCCAGCCGTTGTGGAAATGATGATTACATCGCTGCgcgat                                                                              <  1:1562699‑M1/93‑6 (MQ=255)

          aCGAATTTACGGGCAAGGGTTACAGAGATAGTTGAAAAAGCGGCTAACAATTTGCCAGCCGTTGTGGAAATGATGATTACATCGCTGCgcgat                                                                              >  1:1228775‑M1/1‑88 (MQ=255)

           cGAATTTACGGGCAAGGGTTACAGAGATAGTTGAAAAAGCGGCTAACAATTTGCCAGCCGTTGTGGAAATGATGATTACATCGCTGCgcgata                                                                             >  1:517032‑M1/1‑87 (MQ=255)

           cGAATTTACGGGCAAGGGTTACAGAGATAGTTGAAAAAGCGGCTAACAATTTGCCAGCCGTTGTGGAAATGATGATTACATCGCTGCgcgata                                                                             <  1:3522167‑M1/93‑7 (MQ=255)

            gAATTTACGGGCAAGGGTTACAGAGATAGTTGAAAAAGCGGCTAACAATTTGCCAGCCGTTGTGGAAATGATGATTACATCGCTGCgcgataa                                                                            >  2:62878‑M1/1‑86 (MQ=255)

            gAATTTACGGGCAAGGGTTACAGAGATAGTTGAAAAAGCGGCTAACAATTTGCCAGCCGTTGTGGAAATGATGATTACATCGCTGCgcgataa                                                                            >  1:3124585‑M1/1‑86 (MQ=255)

             aaTTTACGGGCAAGGGTTACAGAGATAGTTGAAAAAGCGGCTAACAATTTGCCAGCCGTTGTGGAAATGATGATTACATCGCTGCgcgataaa                                                                           >  2:1439182‑M1/1‑85 (MQ=255)

             aaTTTACGGGCAAGGGTTACAGAGATAGTTGAAAAAGCGGCTAACAATTTGCCAGCCGTTGTGGAAATGATGATTACATCGCTGCgcgataaa                                                                           >  1:4399158‑M1/1‑85 (MQ=255)

             aaTTTACGGGCAAGGGTTACAGAGATAGTTGAAAAAGCGGCTAACAATTTGCCAGCCGTTGTGGAAATGATGATTACATCGCTGCgcgataaa                                                                           <  1:4172198‑M1/93‑9 (MQ=255)

             aaTTTACGGGCAAGGGTTACAGAGATAGTTGAAAAAGCGGCTAACAATTTGCCAGCCGTTGTGGAAATGATGATTACATCGCTGCgcgataaa                                                                           >  2:2014301‑M1/1‑85 (MQ=255)

              aTTTACGGGCAAGGGTTACAGAGATAGTTGAAAAAGCGGCTAACAATTTGCCAGCCGTTGTGGAAATGATGATTACATCGCTGCgcgataaat                                                                          >  2:3351731‑M1/1‑84 (MQ=255)

              aTTTACGGGAAAGGGTTACAGAGATAGTTGAAAAAGCGGCTAACAATTTGCCAGCCGTTGTGGAAATGATGATTACATCGCTGCgcgataaat                                                                          <  1:1802073‑M1/93‑10 (MQ=255)

               tttACGGGCAAGGGTTACAGAGATAGTTGAAAAAGCGGCTAACAATTTGCCAGCCGTTGTGGAAATGATGATTACATCGCTGCgcgataaatc                                                                         <  1:3406066‑M1/93‑11 (MQ=255)

               tttACGGGCAAGGGTTACAGAAATAGTTGAAAAAGCGGCTAACAATTTGCCAGCCGTTGTGGAAATGATGATTACATCGCTGCgcgataaatc                                                                         <  1:1028944‑M1/93‑11 (MQ=255)

                ttACGGGCAAGGGTTACAGAGATAGTTGAAAAAGCGGCTAACAATTTGCCAGCCGTTGTGGAAATGATGATTACATCGCTGCgcgataaatcg                                                                        >  2:2794202‑M1/1‑82 (MQ=255)

                    gggCAAGGGTTACAGAGATAGTTGAAAAAGCGGCTAACAATTTGCCAGCCGTTGTGGAAATGATGATTACATCGCTGCgcgataaatcgccac                                                                    <  2:3509222‑M1/93‑16 (MQ=255)

                      gCAAGGGTTACAGAGATAGTTGAAAAAGCGGCTAACAATTTGCCAGCCGTTGTGGAAATGATGATTACATCGCTGCgcgataaatcgccacaa                                                                  >  1:3065214‑M1/1‑76 (MQ=255)

                      gCAAGGGTTACAGAGATAGTTGAAAAAGCGGCTAACAATTTGCCAGCCGTTGTGGAAATGATGATTACATCGCTGCgcgataaatcgccacaa                                                                  >  2:2462629‑M1/1‑76 (MQ=255)

                      gCAAGGGTTACAGAGATAGTTGAAAAAGCGGCTAACAATTTGCCAGCCGTTGTGGAAATGATGATTACATCGCTGCgcgataaatcgccacaa                                                                  <  2:2080522‑M1/93‑18 (MQ=255)

                        aaGGGTTACAGAGATAGTTGAAAAAGCGGCTAACAATTTGCCAGCCGTTGTGGAAATGATGATTACATCGCTGCgcgataaatcgccacaatt                                                                <  1:652761‑M1/93‑20 (MQ=255)

                          gggTTACAGAGATAGTTGAAAAAGCGGCTAATAATTTGCCAGCCGTTGTGGAAATGATGATTACATCGCTGCgcgataaatcgccacaatttc                                                              >  1:236995‑M1/1‑72 (MQ=255)

                            gTTACAGAGATAGTTGAAAAAGCGGCTAACAATTTGCCAGCCGTTGTGGAAATGATGATTACATCGCTGCgcgataaatcgccacaatttctt                                                            <  1:2776941‑M1/93‑24 (MQ=255)

                            gTTACAGAGATAGTTGAAAAAGCGGCTAACAATTTGCCAGCCGTTGTGGAAATGATGATTACATCGCTGCgcgataaatcgccacaatttctt                                                            >  2:4099480‑M1/1‑70 (MQ=255)

                             ttACAGAGATAGTTGAAAAAGCGGCTAACAATTTGCCAGCCGTTGTGGAAATGATGATTACATCGCTGCgcgataaatcgccacaatttcttc                                                           <  1:160606‑M1/93‑25 (MQ=255)

                             ttACAGAGATAGTTGAAAAAGCGGCTAACAATTTGCCAGCCGTTGTGGAAATGATGATTACATCGCTGCgcgataaatcgccacaatttcttc                                                           <  2:3137260‑M1/93‑25 (MQ=255)

                             ttACAGAGATAGTTGAAAAAGCGGCTAACAATTTGCCAGCCGTTGTGGAAATGATGATTACATCGCTGCgcgataaatcgccacaatttcttc                                                           <  1:2853526‑M1/93‑25 (MQ=255)

                                 agagATAGTTGAAAAAGCGGCTAACAATTTGCCAGCCGTTGTGGAAATGATGATTACATCGCTGCgcgataaatcgccacaatttcttcgtga                                                       >  1:4098702‑M1/1‑65 (MQ=255)

                                  gaaatagTTGAAAAAGCGGCTAACAATTTGCCAGCCGTTGTGGAAATGATGATTACATCGCTGCgcgataaatcgccacaatttcttcgtgag                                                      <  1:2992604‑M1/90‑30 (MQ=255)

                                   agaTAGTTGAAAAAGCGGCTAACAATTTGCCAGCCGTTGTGGAAATGATGATTACATCGCTGCgcgataaatcgccacaatttcttcgtgagt                                                     <  2:1311344‑M1/93‑31 (MQ=255)

                                   agaTAGTTGAAAAAGCGGCTAACAATTTGCCAGCCGTTGTGGAAATGATGATTACATCGCTGCgcgataaatcgccacaatttcttcgtgagt                                                     >  1:1100647‑M1/1‑63 (MQ=255)

                                    aatAGGTGAAAAAACGGCTAACAATTTGCCAGCCGTTGTGGAAATGATGATTACATCGCTGCgcgataaatcgccacaatttcttcgtgagtt                                                    <  1:836202‑M1/92‑32 (MQ=255)

                                     aTAGTTGAAAAAGCGGCTAACAATTTGCCAGCCGTTGTGGAAATGATGATTACATCGCTGCgcgataaatcgccacaatttcttcgtgagttg                                                   >  1:2412762‑M1/1‑61 (MQ=255)

                                      tAGTTGAAAAAGCGGCTAACAATTTGCCAGCCGTTGTGGAAATGATGATTACATCGCTGCgcgataaatcgccacaatttcttcgtgagttgc                                                  >  1:1373328‑M1/1‑60 (MQ=255)

                                      tAGTTGAAAAAGCGGCTAACAATTTGCCAGCCGTTGTGGAAATGATGATTACATCGCTGCgcgataaatcgccacaatttcttcgtgagttgc                                                  >  2:4746245‑M1/1‑60 (MQ=255)

                                         ttGAAAAAGCGGCTAACAATTTGCCAGCCGTTGTGGAAATGATGATTACATCGCTGCgcgataaatcgccacaatttcttcgtgagttgcctg                                               <  2:4143205‑M1/93‑37 (MQ=255)

                                            aaaaaGCGGCTAACAATTTGCCAGCCGTTGTGGAAATGATGATTACATCGCTGCgcgataaatcgccacaatttcttcgtgagttgcctggat                                            <  1:739416‑M1/93‑40 (MQ=255)

                                             aaaaGCGGCTAACAATTTGCCAGCCGTTGTGGAAATGATGATTACATCGCTGCgcgataaatcgccacaatttcttcgtgagttgcctggatc                                           >  1:1464126‑M1/1‑53 (MQ=255)

                                             aaaaGCGGCTAACAATTTGCCAGCCGTTGTGGAAATGATGATTACATCGCTGCgcgataaatcgccacaatttcttcgtgagttgcctggatc                                           >  1:1344258‑M1/1‑53 (MQ=255)

                                             aaaaGCGGCTAACAATTTGCCAGCCGTTGTGGAAATGATGATTACATCGCTGCgcgataaatcgccacaatttcttcgtgagttgcctggatc                                           <  1:47624‑M1/93‑41 (MQ=255)

                                              aaaGGGGCTAACAATTTGCCAGCCGTTGTGGAAATGATGATTACATCGCTGCgcgataaatcgccacaatttcttcgtgagttgcctggatcg                                          <  1:396552‑M1/93‑42 (MQ=255)

                                              aaaGCGGCTAACAATTTGCCAGCCGTTGTGGAAATGATGATTACATCGCTGCgcgataaatcgccacaatttcttcgtgagttgcctggatcg                                          >  1:1397525‑M1/1‑52 (MQ=255)

                                               aaGCGGCTAACAATTTGCCAGCCGTTGTGGAAATGATGATTACATCGCTGCgcgataaatcgccacaatttcttcgtgagttgcctggatcgg                                         <  2:1713620‑M1/93‑43 (MQ=255)

                                                atcggCTAACAATTTGCCAGCCGTTGTGGAAATGATGATTACATCGCTGCgcgataaatcgccacaatttcttcgtgagttgcctggatcggg                                        >  2:2377312‑M1/3‑50 (MQ=255)

                                                  cGGCTAACAATTTGCCAGCCGTTGTGGAAATGATGATTACATCGCTGCgcgataaatcgccacaatttcttcgtgagttgcctggatcgggtt                                      <  2:3698410‑M1/93‑46 (MQ=255)

                                                    ggtaaCAATTTGCCAGCCGTTGTGGAAATGATGATTACATCGCTGCgcgataaatcgccacaatttcttcgtgagttgcctggatcgggttag                                    >  1:2332854‑M1/3‑46 (MQ=255)

                                                    gCTAACAATTTGCCAGCCGTTGTGGAAATGATGATTACATCGCTGCgcgataaatcgccacaatttcttcgtgagttgcctggatcgggttag                                    <  1:723061‑M1/93‑48 (MQ=255)

                                                    gCTAACAATTTGCCAGCCGTTGTGGAAATGATGATTACATCGCTGCgcgataaatcgccacaatttcttcgtgagttgcctggatcgggttag                                    <  2:3709020‑M1/93‑48 (MQ=255)

                                                     cTAACAATTTGCCAGCCGTTGTGGAAATGATGATTACATCGCTGCgcgataaatcgccacaatttcttcgtgagttgcctggatcgggttagt                                   <  2:274004‑M1/93‑49 (MQ=255)

                                                        aCAATTTGCCAGCCGTTGTGGAAATGATGATTACATCGCTGCgcgataaatcgccacaatttcttcgtgagttgcctggatcgggttagtaaa                                >  1:1252496‑M1/1‑42 (MQ=255)

                                                        aCAATTTGCCAGCCGTTGTGGAAATGATGATTACATCGCTGCgcgataaatcgccacaatttcttcgtgagttgcctggatcgggttagtaaa                                <  1:192039‑M1/93‑52 (MQ=255)

                                                        aCAATTTGCCAGCCGTTGTGGAAATGATGATTACATCGCTGCgcgataaatcaccacaatttcttcgtgagttgcctggatcgggttagtaaa                                <  1:605060‑M1/93‑52 (MQ=255)

                                                          aaTTTGCCAGCCGTTGTGGAAATGATGATTACATCGCTGCgcgataaatcgccacaatttcttcgtgagttgcctggatcgggttagtaaagc                              >  1:2681235‑M1/1‑40 (MQ=255)

                                                           aTTTGCCAGCCGTTGTGGAAATGATGATTACATCGCTGCgcgataaatcgccacaatttcttcgtgagttgcctggatcgggttagtaaagcc                             >  1:1706307‑M1/1‑39 (MQ=255)

                                                              tGCCAGCCGTTGTGGAAATGATGATTACATCGCTGCgcgataaatcgccacaatttcttcgtgagttgcctggatcgggttagtaaagccaca                          <  1:3263087‑M1/93‑58 (MQ=255)

                                                              tGCCAGCCGTTGTGGAAATGATGATTACATCGCTGCgcgataaatcgccacaatttcttcgtgagttgcctggatcgggttagtaaagccaca                          <  1:2443989‑M1/93‑58 (MQ=255)

                                                              tGCCAGCCGTTGTGGAAATGATGATTACATCGCTGCgcgataaatcgccacaatttcttcgtgagttgcctggatcgggttagtaaagccaca                          <  2:3842781‑M1/93‑58 (MQ=255)

                                                              tGCCAGCCGTTGTGGAAATGATGATTACATCGCTGCgcgataaatcgccacaatttcttcgtgagttgcctggatcgggttagtaaagccaca                          <  2:1209861‑M1/93‑58 (MQ=255)

                                                              tGCCAGCCGTTGTGGAAATGATGATTACATCGCTGCgcgataaatcgccacaatttcttcgtgagttgcctggaccgggttagtaaagccaca                          <  2:4644020‑M1/93‑58 (MQ=255)

                                                               tccaGCCGTTGTGGAAATGATGATTACATCGCTGCgcgataaatcgccacaatttcttcgtgagttgcctggatcgggttagtaaagccacag                         <  1:3611167‑M1/92‑59 (MQ=255)

                                                               gCCAGCCGTTGTGGAAATGATGATTACATCGCTGCgcgataaatcgccacaatttcttcgtgagttgcctggatcgggttagtaaagccacag                         <  1:3365338‑M1/93‑59 (MQ=255)

                                                               gCCAGCCGTTGTGGAAATGATGATTACATCGCTGCgcgataaatcgccacaatttcttcgtgagttgcctggatcgggttagtaaagccacag                         <  1:3879740‑M1/93‑59 (MQ=255)

                                                                ccAGCCGTTGTGGAAATGATGATTACATCGCTGCgcgataaatcgccacaatttcttcgtgagttgcctggatcgggttagtaaagccacagg                        <  1:3527464‑M1/93‑60 (MQ=255)

                                                                 cAGCCGTTGTGGAAATGATGATTACATCGCTGCgcgataaatcgccacaatttcttcgtgagttgcctggatcgggttagtaaaggcacaggc                       >  2:2150470‑M1/1‑33 (MQ=255)

                                                                 cAGCCGTTGTGGAAATGATGATTACATCGCTGCgcgataaatcgccacaatttcttcgtgagttgcctggatcgggttagtaaagccacaggc                       <  2:778577‑M1/93‑61 (MQ=255)

                                                                 cAGCCGTTGTGGAAATGATGATTACATCCCTGCgcgataaatcgccacaatttcttcgtgagttgcctggatcgggttagtaaagccacaggc                       <  1:1828366‑M1/93‑61 (MQ=255)

                                                                  aGCCGTTGTGGAAATGATGATTACATCGCTGCgcgataaatcgccacaatttcttcgtgagttgcctggatcgggttagtaaagccacaggca                      <  2:433307‑M1/93‑62 (MQ=255)

                                                                     cGTTGTGGAAATGATGATTACATCGCTGCgcgataaatcgccacaatttcttcgtgagttgcctggatcgggttagtaaagccacaggcatct                   <  1:469854‑M1/93‑65 (MQ=255)

                                                                     cGTTGTGGAAATGATGATTACATCGCTGCgcgataaatcgccacaatttcttcgtgagttgcctggatcgggttagtaaagccacaggcatct                   >  1:2150195‑M1/1‑29 (MQ=255)

                                                                       ttGTGGAAATGATGATTACATCGCTGCgcgataaatcgccacaatttcttcgtgagttgcctggatcgggttagtaaagccacaggcatcttt                 >  2:3072735‑M1/1‑27 (MQ=255)

                                                                         gtgGAAATGATGATTACATCGCTGCgcgataaatcgccacaatttcttcgtgagttgcctggatcgggttagtaaagccacaggcatctttca               >  2:1153180‑M1/1‑25 (MQ=255)

                                                                         gtgGAAATGATGATTACATCGCTGCgcgataaatcgccacaatttcttcgtgagttgcctggatcgggttagtaaagccacaggcatctttca               <  2:854904‑M1/93‑69 (MQ=255)

                                                                          tgGAAATGATGATTACATCGCTGCgcgataaatcgccacaatttcttcgtgagttgcctggatcgggttagtaaagccacaggcatctttcag              <  1:4619048‑M1/93‑70 (MQ=255)

                                                                          tgGAAATGATGATTACATCGCTGCgcgataaatcgccacaatttcttcgtgagttgcctggatcgggttagtaaagccacaggcatctttcag              <  1:4498805‑M1/93‑70 (MQ=255)

                                                                            gAAATGATGATTACATCGCTGCgcgataaatcgccacaatttcttcgtgagttgcctggatcgggttagtaaagccacaggcatctttcaggg            >  1:4268686‑M1/1‑22 (MQ=255)

                                                                             aaaTGATGATTACATCGCTGCgcgataaatcgccacaatttcttcgtgagttgcctggatcgggttagtaaagccacaggcatctttcagggc           >  2:3020800‑M1/1‑21 (MQ=255)

                                                                              aaTGATGATTACATCGCTGCgcgataaatcgccacaatttcttcgtgagttgcctggatcgggttagtaaagccacaggcatctttcagggca          >  2:2719144‑M1/1‑20 (MQ=255)

                                                                               atgatgATTACATCGCTGCgcgataaatcgccacaatttcttcgtgagttgcctggatcgggttagtaaagccacaggcatctttcagggcat         <  2:4516198‑M1/93‑75 (MQ=255)

                                                                                tgatgaTTACATCGCTGCgcgataaatcgccacaatttcttcgtgagttgcctggatcgggttagtaaagccacaggcatctttcagggcatt        >  1:967194‑M1/1‑18 (MQ=255)

                                                                                 gatgatTACATCGCTGCgcgataaatcgccacaatttcttcgtgagttgcctggatcgggttagtaaagccacaggcatctttcagggcatta       >  2:621403‑M1/1‑17 (MQ=255)

                                                                                 gatgatTACATCGCTGCgcgataaatcgccacaatttcttcgtgagttgcctggatcgggttagtaaagccacaggcatctttcagggcatta       <  1:1883508‑M1/93‑77 (MQ=255)

                                                                                  atgatTACATCGCTGCgcgataaatcgccacaatttcttcgtgagttgcctggatcgggttagtaaagccacaggcatctttcagggcattag      <  1:141927‑M1/93‑78 (MQ=255)

                                                                                  atgatTACATCGCTGCgcgataaatcgccacaatttcttcgtgagttgcctggatcgggttagtaaagccacaggcatctttcagggcattag      <  2:1421052‑M1/93‑78 (MQ=255)

                                                                                  atgatTACATCGCTGCgcgataaatcgccacaatttcttcgtgagttgcctggatcgggttagtaaagccacaggcatctttcagggcattag      <  1:901781‑M1/93‑78 (MQ=255)

                                                                                   tgatTACATCGCTGCgcgataaatcgccacaatttcttcgtgagttgcctggatcgggttagtaaagccacaggcatctttcagggcattagt     <  1:105688‑M1/93‑79 (MQ=255)

                                                                                    gatTACATCGCTGCgcgataaatcgccacaatttcttcgtgagttgcctggatcgggttagtaaagccacaggcatctttcagggcattagtc    >  2:1819879‑M1/1‑14 (MQ=255)

                                                                                    gatTACATCGCTGCgcgataaatcgccacaatttcttcgtgagttgcctggatcgggttagtaaagccacaggcatctttcagggcattagtc    >  1:1209727‑M1/1‑14 (MQ=255)

                                                                                     atTACATCGCTGCgcgataaatcgccacaatttcttcgtgagttgcctggatcgggttagtaaagccacaggcatctttcagggcattagtcg   >  1:850776‑M1/1‑13 (MQ=255)

                                                                                      ttACATCGCTGCgcgataaatcgccacaatttcttcgtgagttgcctggatcgggttagtaaagccacaggcatctttcagggcattagtcgc  <  2:2050461‑M1/93‑82 (MQ=255)

                                                                                      ttACATCGCTGCgcgataaatcgccacaatttcttcgtgagttgcctggatcgggttagtaaagccacaggcatctttcagggcattagtcgc  >  2:886190‑M1/1‑12 (MQ=255)

GACAGCTATCACGAATTTACGGGCAAGGGTTACAGAGATAGTTGAAAAAGCGGCTAACAATTTGCCAGCCGTTGTGGAAATGATGATTACCTCGCTGCATAAATCGCCACAATTTCTTCGTGAGTTGCCTGGATCGGGTTAGTAAAGCCACAGGCATCTTTCAGGGCATTAGTCGCCAA  >  NC_000913/3754220‑3754398

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 24 ≤` `ATCG/ATCG` `< 30 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`