BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,775,779	A→G	I252V (ATT→GTT)	lldP →	L‑lactate permease

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,775,779	0	A	G	99.3%	481.4 / NA	147	I252V (ATT→GTT)	lldP	L‑lactate permease
Reads supporting (aligned to +/- strand): ref base A (0/1); new base G (54/92); total (54/93)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 151 total aligned reads displayed.

AAGAGACGTGGCCTGCGGTCGTGGTTGCGGGCGGCTCGTTTGCCATCGCTCAGTACCTTAGCTCTAACTTCATTGGGCCGGAGCTGCCGGACATTATCTCTTCGCTGGTATCACTGCTCTGCCTGACGCTGTTCCTCAAACGCTGGCAGCCAGTGCGTGTATTCCGTTTTGGTGATTTGGGG  >  NC_000913/3775687‑3775868

aaGAGACGTGGCCTGCTGTCGTGGTTGCGGGCGGCTCGTTTGCCATCGCTCAGTACCTTAGCTCTAACTTCATTGGGCCGGAGCTGCCGGACa                                                                                           <  2:4036560/93‑1 (MQ=255)

  gagaCGTGGCCTGCGGTCGTGGTTGCGTGCGGCTCGTTTGCCATCGCTCAGTACCTTAGCTCTAACTTCATTGGGCCGGAGCTGCCGGACGtt                                                                                         <  2:3289139/93‑1 (MQ=255)

   agaCGTGGCCTGCGGTCGTGGTTGCGGGCGGCTCGTTTGCCATCGCTCAGTACCTTAGCTCTAACTTCATTGGGCCGGAGCTGCCGGACGTTa                                                                                        <  2:3682296/93‑1 (MQ=255)

   agaCGTGGCCTGCGGTCGTGGTTGCGGGCGGCTCGTTTGCCATCGCTCAGTACCTTAGCTCTAACTTCATTGGGCCGGAGCTGCCGGACGTTa                                                                                        <  2:3498073/93‑1 (MQ=255)

    gaCGTGGCCTGCGGTCGTGGTTGCGGGCAGCTCGTTTGCCATCGCTCAGTACCTTAGCTCTAACTTCATTGGGCCGGAGCTGCCGGACGTTAt                                                                                       <  2:2901192/93‑1 (MQ=255)

       gTGGCCTGCGGTCGTGGTTGCGGGCGGCTCGTTTGCCATCGCTCAGTACCTTAGCTCTAACTTCATTGGGCCGGAGCTGCCGGACGTTAtctc                                                                                    >  2:571965/1‑93 (MQ=255)

        tGGCCTGCGGTCGTGGTTGCGGGCGGCTCGTTTGCCATCGCTCAGTACCTTAGCTCTAACTTCATTGGGCCGGAGCTGCCGGACGTTATctct                                                                                   <  2:1962210/93‑1 (MQ=255)

        tGGCCTGCGGTCGTGGTTGCGGGCGGCTCGTTTGCCATCGCTCAGTACCGTAGCTCTAACTTCATTGGGCCGGAGCTGCCGGACGTTATctct                                                                                   <  1:1305412/93‑1 (MQ=255)

        gggCCTGGGGTCGTGGTTGCGGGCGGCTCGTTTGCCATCGCTCAGTACCTTAGCTCTAACTTCATTGGGCCGGAGCTGCTGGACGTTATctct                                                                                   <  2:4030902/92‑1 (MQ=255)

         ggCCTGCGGTCGTGGTTGCGGGCGGCTCGTTTGCCATCGCTCAGTACCTTAGCTCTAACTTCATTGGGCCGGAGCTGCCGGACGTTATCTCtt                                                                                  <  1:4158183/93‑1 (MQ=255)

         ggCCTGCGGTCGTGGTTGCGGGCGGCTCGTTTGCCATCGCTCAGTACCTTAGCTCTAACTTCATTGGGCCGGAGCTGCCGGACGTTATCTCtt                                                                                  <  2:1083237/93‑1 (MQ=255)

         ggCCTGCGGTCGTGGTTGCGGGCGGCTCGTTTGCCATCGCTCAGTACCTTAGCTCTAACTTCATTGGGCCGGAGCTGCCGGACGTTATCTCtt                                                                                  >  1:2916447/1‑93 (MQ=255)

         ggCCTGCGGTCGTGGTTGCGGGCGGCTCGTTTGCCATCGCTCAGTACCTTAGCTCTAACTTCATTGGGCCGGAGCTGCCGGACGTTATCTCtt                                                                                  >  2:309717/1‑93 (MQ=255)

         ggCCTGCGGTCGTGGTTGCGGGCGGCTCGTTTGCCATCGCTCAGTACCTTAGCTCTAACTTCATTGGGCCGGAGCTGCCGGACGTTATCTCtt                                                                                  <  2:4053242/93‑1 (MQ=255)

         ggCCTGCGGTCGTGGTTGCGGGCGGCTCGTTTGCCATCGCTCAGTACCTTAGCTCTAACTTCATTGGGCCGGAGCTGCCGGACGTTATCTCtt                                                                                  >  2:521431/1‑93 (MQ=255)

             tGCGGTCGTGGTTGCGGGCGGCTCGTTTGCCATCGCTCAGTACCTTAGCTCTAACTTCATTGGGCCGGAGCTGCCGGACGTTATCTCTTCGCt                                                                              <  1:4672456/93‑1 (MQ=255)

                ggTCGTGGTTGCGGGCGGCTCGTTTGCCATCGCTCAGTACCTTAGCTCTAACTTCATTGGGCCGGAGCTGCCGGACGTTATCTCTTCGCTGGt                                                                           <  2:1994463/93‑1 (MQ=255)

                 gTCGTGGTTGCGGGCGGCTCGTTTGCCATCGCTCAGTACCTTAGCTCTAACTTCATTGGGCCGGAGCTGCCGGACGTTATCTCTTCGCTGGTa                                                                          <  2:3420240/93‑1 (MQ=255)

                 gTCGTGGTTGCGGGCGGCTCGTTTGCCATCGCTCAGTACCTTAGCTCTAACTTCATTGGGCCGGAGCTGCCGGACGTTATCTCTTCGCTGGTa                                                                          <  1:548107/93‑1 (MQ=255)

                  tCGTGGTTGCGGGCGGCTCGTTTGCCATCGCTCAGTACCTTAGCTCTAACTTCATTGGGCCGGAGCTGCCGGACGTTATCTCTTCGCTGGTAt                                                                         <  1:184029/93‑1 (MQ=255)

                  tCGTGGTTGCGGGCGGCTCGTTTGCCATCGCTCAGTACCTTAGCTCTAACTTCATTGGGCCGGAGCTGCCGGACGTTATCTCTTCGCTGGTAt                                                                         <  1:677983/93‑1 (MQ=255)

                    gTGGTTGCGGGCGGCTCGTTTGCCATCGCTCAGTACCTTAGCTCTAACTTCATTGGGCCGGAGCTGCCGGACGTTATCTCTTCGCTGGTATCa                                                                       >  2:470843/1‑93 (MQ=255)

                     tGGTTGCGGGCGGCTCGTTTGCCATCGCTCAGTACCTTAGCTCTAACTTCATTGGGCCGGAGCTGCCGGACGTTATCTCTTCGCTGGTATCAc                                                                      <  1:237875/93‑1 (MQ=255)

                     tGGTTGCGGGCGGCTCGTTTGCCATCGCTCAGTACCTTAGCTCTAACTTCATTGGGCCGGAGCTGCCGGACGTTATCTCTTCGCTGGTATCAc                                                                      >  2:4332933/1‑93 (MQ=255)

                     tGGTTGCGGGCGGCTCGTTTGCCATCGCTCAGTACCTTAGCTCTAACTTCATTGGGCCGGAGCTGCCGGACGTTATCTCTTCGCTGGTATCAc                                                                      >  1:1156164/1‑93 (MQ=255)

                      ggTTGCGGGCGGCTCGTTTGCCATCGCTCAGTACCTTAGCTCTAACTTCATTGGGCCGGAGCTGCCGGACGTTATCTCTTCGCTGGTATCACt                                                                     <  2:15130/93‑1 (MQ=255)

                      ggTTGCGGGCGGCTCGTTTGCCATCGCTCAGTACCTTAGCTCTAACTTCATTGGGCCGGAGCTGCCGGACGTTATCTCTTCGCTGGTATCACt                                                                     <  1:2659402/93‑1 (MQ=255)

                       gTTGCGGGCGGCTCGTTTGCCATCGCTCAGTACCTTAGCTCTAACTTCATTGGGCCGGAGCTGCCGGACGTTATCTCTTCGCTGGTATCACTg                                                                    <  2:1609104/93‑1 (MQ=255)

                       gTTGCGGGCGGCTCGTTTGCCATCGCTCAGTACCTTAGCTCTAACTTCATTGGGCCAGAGCTGCCGGACGTTATCTCTTCGCTGGTATCACTg                                                                    >  2:3046431/1‑93 (MQ=255)

                        ttGCGGGCGGCTCGTTTGCCATCGCTCAGTACCTTAGCTCTAACTTCATTGGGCCGGAGCTGCCGGACGTTATCTCTTCGCTGGTATCACTGc                                                                   <  2:4157359/93‑1 (MQ=255)

                        ttGCGGGCGGCTCGTTTGCCATCGCTCAGTACCTTAGCTCTAACTTCATTGGGCCGGAGCTGCCGGACGTTATCTCTTCGCTGGTATCACTGc                                                                   <  1:1652034/93‑1 (MQ=255)

                          gcgggcggCTCGTTTGCCATCGCTCAGTACCTTAGCTCTAACTTCATTGGGCCGGAGCTGCCGGACGTTATCTCTTCGCTGGTATCACTGctc                                                                 <  2:1833073/93‑1 (MQ=255)

                          gcgggcggCTCGTTTGCCATCGCTCAGTACCTTAGCTCTAACTTCATTGGGCCGGAGCTGCCGGACGTTATCTCTTCGCTGGTATCACTGctc                                                                 <  2:4558424/93‑1 (MQ=255)

                              gcggcTCGTTTGCCATCGCTCAGTACCTTAGCTCTAACTTCATTGGGCCGGAGCTGCCGGACGTTATCTCTTCGCTGGTATCACTGCTCTGcc                                                             <  2:2482618/93‑1 (MQ=255)

                               cggcTCGTTTGCTATCGCTCAGTACCTTAGCTCTAACTTCATTGGGCCGGAGCTGCCGGACGTTATCTCTTCGCTGGTATCACTGCTCTGCCt                                                            <  2:781809/93‑1 (MQ=255)

                                 gcTCGTTTGCCATCGCTCAGTACCTTAGCTCTAACTTCATTGGGCCGGAGCTGCCGGACGTTATCTCTTCGCTGGTATCACTGCTCTGCCTGa                                                          >  2:662353/1‑93 (MQ=255)

                                 gcTCGTTTGCCATCGCTCAGTACCTTAGCTCTAACTTCATTGGGCCGGAGCTGCCGGACGTTATCTCTTCGCTGGTATCACTGCTCTGCCTGa                                                          <  2:1543730/93‑1 (MQ=255)

                                 gcTCGTTTGCCATCGCTCAGTACCTTAGCTCTAACTTCATTGGGCCGGAGCTGCCGGACGTTATCTCTTCGCTGGTATCACTGCTCTGCCTGa                                                          >  2:2152919/1‑93 (MQ=255)

                                   tCGTTTGCCATCGCTCAGTACCTTAGCTCTAACTTCATTGGGCCGGAGCTGCCGGACGTTATCTCTTCGCTGGTATCACTGCTCTGCCTGACg                                                        >  1:3138113/1‑93 (MQ=255)

                                    cGTTTGCCATCGCTCAGTACCTTAGCTCTAACTTCATTGGGCCGGAGCTGCCGGACGTTATCTCTTCGCTGGTATCACTGCTCTGCCTGACGc                                                       <  1:3098353/93‑1 (MQ=255)

                                     gTTTGCCATCGCTCAGTACCTTAGCTCTAACTTCATTGGGCCGGAGCTGCCGGACGTTATCTCTTCGCTGGTATCACTGCTCTGCCTGACGCt                                                      >  2:1648680/1‑93 (MQ=255)

                                     gTTTGCCATCGCTCAGTACCTTAGCTCTAACTTCATTGGGCCGGAGCTGCCGGACGTTATCTCTTCGCTGGTATCACTGCTCTGCCTGACGCt                                                      >  2:1648679/1‑93 (MQ=255)

                                     gTTTGCCATCGCTCAGTACCTTAGCTCTAACTTCATTGGGCCGGAGCTGCCGGACGTTATCTCTTCGCTGGTATCACTGCTCTGCCTGACGCt                                                      <  1:3576614/93‑1 (MQ=255)

                                     gTTTGCCATCGCTCAGTACCTTAGCTCTAACTTCATTGGGCCGGAGCGGCCGGACGTTATCTCTTCGCTGGTATCACTGCTCTGCCTGATGCt                                                      >  2:3712402/1‑93 (MQ=255)

                                      tttGCCATCGCTCAGTACCTTAGCTCTAACTTCATTGGGCCGGAGCTGCCGGACGTTATCTCTTCGCTGGTATCACTGCTCTGCCTGACGCtt                                                     >  1:1613184/1‑92 (MQ=255)

                                       ttGCCATCGCTCAGTACCTTAGCTCTAACTTCATTGGGCCGGAGCTGCCGGACGTTATCTCTTCGCTGGTATCACTGCTCTGCCTGACGCTGt                                                    <  2:3649405/93‑1 (MQ=255)

                                       ttGCCATCGCTCAGTACCTTAGCTCTAACTTCATTGGGCCGGAGCTGCCGGACGTTATCTCTTCGCTGGTATCACTGCTCTGCCTGACGCTGt                                                    <  2:1076117/93‑1 (MQ=255)

                                       ttGCCATCGCTCAGTACCTTAGCTCTAACTTCATTGGGCCGGAGCTGCCGGACGTTATCTCTTCGCTGGTATCACTGCTCTGCCTGACGCTGt                                                    <  2:2708826/93‑1 (MQ=255)

                                       ttGCCATCGCTCAGTACCTTAGCTCTAACTTCATTGGGCCGGAGCTGCCGGACGTTATCTCTTCGCTGGTATCACTGCTCTGCCTGACGCTGt                                                    >  1:1384739/1‑93 (MQ=255)

                                       ttGCCATCGCTCAGTACCTTAGCTCTAACGTCATTGGGCCGGAGCTGCCGGACGTTATCTCTTCGCTGGTATCACTGCTCTGCCTGACGCTGt                                                    <  1:749833/93‑1 (MQ=255)

                                           cATCGCTCAGTACCTTAGCTCTAACTTCATTGGGCCGGAGCTGCCGGACGTTATCTCTTCGCTGGTATCACTGCTCTGCCTGACGCTGTTCCt                                                <  2:4579179/93‑1 (MQ=255)

                                            aTCGCTCAGTACCTTAGCTCTAACTTCATTGGGCCGGAGCTGCCGGACGTTATCTCTTCGCTGGTATCACTGCTCTGCCTGACGCTGTTCCTc                                               <  2:4730731/93‑1 (MQ=255)

                                             tCGCTCAGTACCTTAGCTCTAACTTCATTGGGCCGGAGCTGCCGGACGTTATCTCTTCGCTGGTATCACTGCTCTGCCTGACGCTGTTCCTCa                                              <  1:2898585/93‑1 (MQ=255)

                                              cGCTCAGTACCTTAGCTCTAACTTCATTGGGCCGGAGCTGCCGGACGTTATCTCTTCGCTGGTATCACTGCTCTGCCTGACGCTGTTCCTCaa                                             >  1:2615739/1‑93 (MQ=255)

                                               gCTCAGTACCTTAGCTCTAACTTCATTGGGCCGGAGCTGCCGGACGTTATCTCTTCGCTGGTATCACTGCTCTGCCTGACGCTGTTCCTCaaa                                            <  1:3050993/93‑1 (MQ=255)

                                               gCTCAGTACCTTAGCTCTAACTTCATTGGGCCGGAGCTGCCGGACGTTATCTCTTCGCTGGTATCACTGCTCTGCCTGACGCTGTTCCTCaaa                                            <  2:799038/93‑1 (MQ=255)

                                                 tCAGTACCTTAGCTCTAACTTCATTGGGCCGGAGCTGCCGGACGTTATCTCTTCGCTGGTATCACTGCTCTGCCTGACGCTGTTCCTCAAACg                                          >  1:3845943/1‑93 (MQ=255)

                                                  cAGTACCTTAGCTCTAACTTCATTGGGCCGGAGCTGCCGGACGTTATCTCTTCGCTGGTATCACTGCTCTGCCTGACGCTGTTCCTCAAACGc                                         <  1:1546283/93‑1 (MQ=255)

                                                  cAGTACCTTAGCTCTAACTTCATTGGGCCGGAGCTGCCGGACGTTATCTCTTCGCTGGTATCACTGCTCTGCCTGACGCTGTTCCTCAAACGc                                         >  1:4599704/1‑93 (MQ=255)

                                                   aGTACCTTAGCTCTAACTTCATTGGGCCGGAGCTGCCGGACGTTATCTCTTCGCTGGTATCACTGCTCTGCCTGACGCTGTTCCTCAAACGCt                                        >  2:4500150/1‑93 (MQ=255)

                                                    gTACCTTAGCTCTAACTTCATTGGGCCGGAGCTGCCGGACGTTATCTCTTCGCTGGTATCACTGCTCTGCCTGACGCTGTTCCTCAAACGCTg                                       <  2:2726324/93‑1 (MQ=255)

                                                    gTACCTTAGCTCTAACTTCATTGGGCCGGAGCTGCCGGACGTTATCTCTTCGCTGGTATCACTGCTCTGCCTGACGCTGTTCCTCAAACGCTg                                       <  2:3848202/93‑1 (MQ=255)

                                                       ccTTAGCTCTAACTTCATTGGGCCGGAGCTGCCGGACGTTATCTCTTCGCTGGTATCACTGCTCTGCCTGACGCTGTTCCTCAAACGCTGGCa                                    <  2:1881275/93‑1 (MQ=255)

                                                       ccTTAGCTCTAACTTCATTGGGCCGGAGCTGCCGGACGTTATCTCTTCGCTGGTATCACTGCTCTGCCTGACGCTGTTCCTCAAACGCTGGCa                                    >  1:995151/1‑93 (MQ=255)

                                                         ttAGCTCTAACTTCATTGGGCCGGAGCTGCCGGACGTTATCTCTTCGCTGGTATCACTGCTCTGCCTGACGCTGTTCCTCAAACGCTGGCCGc                                  >  1:1350909/1‑93 (MQ=255)

                                                         ttAGCTCTAACTTCATTGGGCCGGAGCTGCCGGACGTTATCTCTTCGCTGGTATCACTGCTCTGCCTGACGCTGTTCCTCAAACGCTGGCAGc                                  >  1:1126211/1‑93 (MQ=255)

                                                         ttAGCTCTAACTTCATTGGGCCGGAGCTGCCGGACGTTATCTCTTCGCTGGTATCACTGCTCTGCCTGACGCTGTTCCTCAAACGCTGGCAGc                                  >  1:2783982/1‑93 (MQ=255)

                                                          tAGCTCTAACTTCATTGGGCCGGAGCTGCCGGACGTTATCTCTTCGCTGGTATCACTGCTCTGCCTGACGCTGTTCCTCAAACGCTGGCAGcc                                 <  2:1920880/93‑1 (MQ=255)

                                                            gCTCTAACTTCATTGGGCCGGAGCTGCCGGACGTTATCTCTTCGCTGGTATCACTGCTCTGCCTGACGCTGTTTCTCAAGCGCTGGCAGTCAg                               >  2:2563817/1‑93 (MQ=255)

                                                             ctctAACTTCATTGGGCCGGAGCTGCCGGACGTTATCTCTTCGCTGGTATCACTGCTCTGCCTGACGCTGTTCCTCAAACGCTGGCAGCCAGt                              <  2:1323621/93‑1 (MQ=255)

                                                                tAACTTCATTGGGCCGGAGCTGCCGGACGTTATCTCTTCGCTGGTATCACTGCTCTGCCTGACGCTGTTCCTCAAACGCTGGCAGCCAGTGCg                           >  1:4247484/1‑93 (MQ=255)

                                                                tAACTTCATTGGGCCGGAGCTGCCGGACGTTATCTCTTCGCTGGTATCACTGCTCTGCCTGACGCTGTTCCTCAAACGCTGGCAGCCAGTGCg                           >  1:2858971/1‑93 (MQ=255)

                                                                 aaCTTCATTGGGCCGGAGCTGCCGGACGTTATCTCTTCGCTGGTATCACTGCTCTGCCTGACGCTGTTCCTCAAACGCTGGCAGCCAGTGCgt                          <  2:4744256/93‑1 (MQ=255)

                                                                    ttCATTGGGCCGGAGCTGCCGGACGTTATCTCTTCGCTGGTATCACTGCTCTGCCTGACGCTGTTCCTCAAACGCTGGCGGCCAGTGCGGGTa                       >  1:1481634/1‑93 (MQ=255)

                                                                    ttCATTGGGCCGGAGCTGCCGGACGTTATCTCTTCGCTGGTATCACTGCTCTGCCTGACGCTGTTCCTCAAACGCTGGCAGCCAGTGCGTGTa                       >  2:3560454/1‑93 (MQ=255)

                                                                     tCATTGGGCCGGAGCTGCCGGACGTTATCTCTTCGCTGGTATCACTGCTCTGCCTGACGCTGTTCCTCAAACGCTGGCAGCCAGTGCGTGTAt                      >  1:906941/1‑93 (MQ=255)

                                                                      cATTGGGCCGGAGCTGCCGGACGTTATCTCTTCGCTGGTATCACTGCTCTGCCTGACGCTGTTCCTCAAACGCTGGCAGCCAGTGCGTGTAtt                     <  2:3878476/93‑1 (MQ=255)

                                                                          gggCCGGAGCTGCCGGACGTTATCTCTTCGCTGGTATCACTGCTCTGCCTGACGCTGTTCCTCAAACGCTGGCAGCCAGTGCGTGTATTCCGt                 <  2:353715/93‑1 (MQ=255)

                                                                          gggCCGGAGCTGCCGGACGTTATCTCTTCGCTGGTATCACTGCTCTGCCTGACGCTGTTCCTCAAACGCTGGCAGCCAGTGCGTGTATTCCGt                 <  1:3914904/93‑1 (MQ=255)

                                                                           ggCCGGAGCTGCCGGACGTTATCTCTTCGCTGGTATCACTGCTCTGCCTGACGCTGTTCCTCAACGGCTGGCAGCCAGTGCGTGTATTCCggt                >  1:3309947/1‑91 (MQ=255)

                                                                           ggCCGGAGCTGCCGGACGTTATCTCTTCGCTGGTATCACTGCTCTGCCTGACGCTGTTCCTCAAACGCTGGCAGCCAGTGCGTGTATTCCGtt                <  1:3637030/93‑1 (MQ=255)

                                                                           ggCCGGAGCTGCCGGACGTTATCTCTTCGCTGGTATCACTGCTCTGCCTGACGCTGTTCCTCAAACGCTGGCAGCCAGTGCGTGTATTCCGtt                <  1:381371/93‑1 (MQ=255)

                                                                            gCCGGAGCTGCCGGACGTTATCTCTTCGCTGGTATCACTGCTCTGCCTGACGCTGTTCCTCAAACGCTGGCAGCCAGTGCGTGTATTCCGttt               <  1:1334815/93‑1 (MQ=255)

                                                                             ccGGAGCTGCCGGACGTTATCTCTTCGCTGGTATCACTGCTCTGCCTGACGCTGTTCCTCAAACGCTGGCAGCCAGTGCGTGTATTCCGtttt              <  1:2502948/93‑1 (MQ=255)

                                                                              cGGAGCTGCCGGACGTTATCTCTTCGCTGGTATCACTGCTCTGCCTGACGCTGTTCCTCAAACGCTGGCAGCCAGTGCGTGTATTCCGTTTTg             <  2:1837429/93‑1 (MQ=255)

                                                                               ggAGCTGCCGGACGTTATCTCTTCGCTGGTATCACTGCTCTGCCTGACGCTGTTCCTCAAACGCTGGCAGCCAGTGCGTGTATTCCGTTTTgg            <  2:2862004/93‑1 (MQ=255)

                                                                                gAGCTGCCGGACGTTATCTCTTCGCTGGTATCACTGCTCTGCCTGACGCTGTTCCTCAAACGCTGGCAGCCAGTGCGTGTATTCCGTTTTGGt           >  1:4540695/1‑93 (MQ=255)

                                                                                gAGCTGCCGGACGTTATCTCTTCGCTGGTATCACTGCTCTGCCTGACGCTGTTCCTCAAACGCTGGCAGCCAGTGCGTGTATTCCGTTTTGGt           >  1:4519315/1‑93 (MQ=255)

                                                                                  gCTGCCGGACGTTATCTCTTCGCTGGTATCACTGCTCTGCCTGACGCTGTTCCTCAAACGCTGGCAGCCAGTGCGTGTATTCCGTTTTGGTGa         <  2:3771400/93‑1 (MQ=255)

                                                                                    tGCCGGACGTTATCTCTTCGCTGGTATCACTGCTCTGCCTGACGCTGTTCCTCAAACGCTGGCAGCCAGTGCGTGTATTCCGTTTTGGTGAtt       <  1:4572380/93‑1 (MQ=255)

                                                                                      ccGGACGTTATCTCTTCGCTGGTATCACTGCTCTGCCTGACGCTGTTCCTCAAACGCTGGCAGCCAGTGCGTGTATTCCGTTTTGGTGATTTg     <  2:1155103/93‑1 (MQ=255)

                                                                                       cGGACGTTATCTCTTCGCTGGTATCACTGCTCTGCCTGACGCTGTTCCTCAAACGCTGGCAGCCAGTGCGTGTATTCCGTTTTGGTGATTTgg    <  2:1384739/93‑1 (MQ=255)

                                                                                        ggACGTTATCTCTTCGCTGGTATCACTGCTCTGCCTGACGCTGTTCCTCAAACGCTGGCAGCCAGTGCGTGTATTCCGTTTTGGTGATTTggg   <  1:1995070/93‑1 (MQ=255)

                                                                                        ggACGTTATCTCTTCGCTGGTATCACTGCTCTGCCTGACGCTGTTCCTCAAACGCTGGCAGCCAGTGCGTGTATTCCGTTTTGGTGATTTggg   <  1:4692896/93‑1 (MQ=255)

                                                                                        ggACGTTATCTCTTCGCTGGTATCACTGCTCTGCCTGACGCTGTTCCTCAAACGCTGGCAGCCAGTGCGTGTATTCCGTTTTGGTGATTTggg   <  1:99524/93‑1 (MQ=255)

                                                                                        ggACGTTATCTCTTCGCTGGTATCACTGCTCTGCCTGACGCTGTTCCTCAAACGCTGGCAGCCAGTGCGTGTATTCCGTTTTGGTGATTTggg   <  2:1177627/93‑1 (MQ=255)

                                                                                        ggACGTTATCTCTTCGCTGGTATCACTGCTCTGCCTGACGCTGTTCCTCAAACGCTGGCAGCCAGTGCGTGTATTCCGTTTTGGTGATTTggg   >  2:2145165/1‑93 (MQ=255)

                                                                                         gACGTTATCTCTTCGCTGGTATCACTGCTCTGCCTGACGCTGTTCCTCAAACGCTGGCAGCCAGTGCGTGTATTCCGTTTTGGTGATTTgggg  >  1:4256742/1‑93 (MQ=255)

                                                                                         gACGTTATCTCTTCGCTGGTATCACTGCTCTGCCTGACGCTGTTCCTCAAACGCTGGCAGCCAGTGCGTGTATTCCGTTTTGGTGATTTgggg  >  2:3369375/1‑93 (MQ=255)

                                                                                         gACGTTATCTCTTCGCTGGTATCACTGCTCTGCCTGACGCTGTTCCTCAAACGCTGGCAGCCAGTGCGTGTATTCCGTTTTGGTGATTTgggg  <  2:919962/93‑1 (MQ=255)

AAGAGACGTGGCCTGCGGTCGTGGTTGCGGGCGGCTCGTTTGCCATCGCTCAGTACCTTAGCTCTAACTTCATTGGGCCGGAGCTGCCGGACATTATCTCTTCGCTGGTATCACTGCTCTGCCTGACGCTGTTCCTCAAACGCTGGCAGCCAGTGCGTGTATTCCGTTTTGGTGATTTGGGG  >  NC_000913/3775687‑3775868

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 19 ≤` `ATCG/ATCG` `< 28 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`