BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,776,340	G→A	G439S (GGT→AGT)	lldP →	L‑lactate permease

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,776,340	0	G	A	97.2%	612.1 / 1.4	176	G439S (GGT→AGT)	lldP	L‑lactate permease
Reads supporting (aligned to +/- strand): ref base G (5/0); new base A (93/78); total (98/78)
Fisher's exact test for biased strand distribution p-value = 6.70e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.63e-01

Only 100 of 180 total aligned reads displayed.

ATCTACTCCATCGGTATGGTGCTGGCATTCGCCTTTATTTCGAACTATTCCGGACTGTCATCAACACTGGCGCTGGCACTGGCGCACACCGGTCATGCATTCACCTTCTTCTCGCCGTTCCTCGGCTGGCTGGGGGTATTCCTGACCGGGTCGGATACCTCATCTAACGCCCTGTTCGCC  >  NC_000913/3776250‑3776429

aTCTACTCCATCGGTATGGTGCTGGCATTCGCCTTTATTTCGAACTATTCCGGACTGTCATCAACACTGGCGCTGGCACTGGCGCACACCAGt                                                                                         <  2:1081630/93‑1 (MQ=255)

aTCTACTCCATCGGTATGGTGCTGGCATTCGCCTTTATTTCGAACTATTCCGGACTGTCATCAACACTGGCGCTGGCACTGGCGCACACCAGt                                                                                         >  1:2033955/1‑93 (MQ=255)

 tCTACTCCATCGGTATGGTGCTGGCATTCGCCTTTATTTCGAACTATTCCGGACTGTCATCAACACTGGCGCTGGCACTGGCGCCCACCAGTc                                                                                        >  2:1178437/1‑93 (MQ=255)

 tCTACTCCATCGGTATGGTGCTGGCATTCGCCTTTATTTCGAACTATTCCGGACTGTCATCAACACTGGCGCTGGCACTGGCGCACACCAGTc                                                                                        <  1:4475688/93‑1 (MQ=255)

  cTACTCCATCGGTATGGTGCTGGCATTCGCCTTTATTTCGAACTATTCCGGACTGTCATCAACACTGGCGCTGGCACTGGCGCACACCAGTCa                                                                                       <  1:514569/93‑1 (MQ=255)

  cTACTCCATCGGTATGGTGCTGGCATTCGCCTTTATTTCGAACTATTCCGGACTGTCATCAACACTGGCGCTGGCACTGGCGCACACCAGTCa                                                                                       <  2:4073222/93‑1 (MQ=255)

   tACTCCATCGGTATGGTGCTGGCATTCGCCTTTATTTCGAACTATTCCGGACTGTCATCAACACTGGCGCTGGCACTGGCGCACACCAGTCAt                                                                                      <  2:4165242/93‑1 (MQ=255)

    aCTCCATCGGTATGGTGCTGGCATTCGCCTTTATTTCGAACTATTCCGGACTGTCATCAACACTGGCGCTGGCACTGGCGCACACCAGTCATg                                                                                     <  2:790055/93‑1 (MQ=255)

     cTCCATCGGTATGGTGCTGGCATTCGCCTTTATTTCGAACTATTCCGGACTGTCATCAACCCTGGCGCTGGCACTGGCGCACCCCAGTCATGc                                                                                    <  2:2778100/93‑1 (MQ=255)

      tCCATCGGTATGGTGCTGGCATTCGCCTTTATTTCGAACTATTCCGGACTGTCATCAACACTGGCGCTGGCACTGGCGCACACCAGTCATGCa                                                                                   <  1:3192198/93‑1 (MQ=255)

       ccATCGGTATGGTGCTGGCATTCGCCTTTATTTCGAACTATTCCGGACTGTCATCAACACTGGCGCTGGCACTGGCGCACACCAGTCATGCAt                                                                                  >  1:3599759/1‑93 (MQ=255)

        cATCGGTATGGTGCTGGCATTCGCCTTTATTTCGAACTATTCCGGACTGTCATCAACACTGGCGCTGGCACTGGCGCACACCAGTCATGCAtt                                                                                 >  2:1536835/1‑93 (MQ=255)

          tCGGTATGGTGCTGGCATTCGCCTTTATTTCGAACTATTCCGGACTGTCATCAACACTGGCGCTGGCACTGGCGCACACCAGTCATGCATTCa                                                                               >  1:1114102/1‑93 (MQ=255)

             gTATGGTGCTGGCATTCGCCTTTATTTCGAACTATTCCGGACTGTCATCAACACTGGCGCTGGCACTGGCGCACACCAGTCATGCATTCACct                                                                            <  1:2139814/93‑1 (MQ=255)

             gTATGGTGCTGGCATTCGCCTTTATTTCGAACTATTCCGGACTGTCATCAACACTGGCGCTGGCACTGGCGCACACCAGTCATGCATTCACct                                                                            >  1:4587122/1‑93 (MQ=255)

             gTATGGTGCTGGCATTCGCCTTTATTTCGAACTATTCCGGACTGTCATCAACACTGGCGCTGGCACTGGCGCACACCAGTCATGCATTCACct                                                                            <  1:4474464/93‑1 (MQ=255)

              tATGGTGCTGGCATTCGCCTTTATTTCGAACTATTCCGGACTGTCATCAACACTGGCGCTGGCACTGGCGCACACCAGTCATGCATTCCCctt                                                                           >  2:1226287/1‑93 (MQ=255)

               aTGGTGCTGGCATTCGCCTTTATTTCGAACTATTCCGGACTGTCATCAACACTGGCGCTGGCACTGGCGCACACCAGTCATGCATTCACcttc                                                                          >  1:1416977/1‑93 (MQ=255)

               aTGGTGCTGGCATTCGCCTTTATTTCGAACTATTCCGGACTGTCATCAACACTGGCGCTGGCACTGGCGCACACCAGTCATGCATTCACcttc                                                                          <  1:2332951/93‑1 (MQ=255)

                tGGTGCTGGCATTCGCCTTTATTTCGAACTATTCCGGACTGTCATCAACACTGGCGCTGGCACTGGCGCACACCAGTCATGCATTCACcttct                                                                         >  2:4537668/1‑93 (MQ=255)

                tGGTGCTGGCATTCGCCTTTATTTCGAACTATTCCGGACTGTCATCAACACTGGCGCTGGCACTGGCGCACACCAGTCATGCATTCACcttct                                                                         >  2:1280600/1‑93 (MQ=255)

                tGGTGCTGGCATTCGCCTTTATTTCGAACTATTCCGGACTGTCATCAACACTGGCGCTGGCACTGGCGCACACCAGTCATGCATTCACcttct                                                                         >  1:3440005/1‑93 (MQ=255)

                tGGTGCTGGCATTCGCCTTTATTTCGAACTATTCCGGACTGTCATCAACACTGGCGCTGGCACTGGCGCACACCAGTCATGCATTCACcttct                                                                         >  2:807502/1‑93 (MQ=255)

                 ggTGCTGGCATTCGCCTTTATTTCGAACTATTCCGGACTGTCATCAACACTGGCGCTGGCACTGGCGCACACCAGTCATGCATTCACcttctt                                                                        >  1:3355010/1‑93 (MQ=255)

                  gTGCTGGCATTCGCCTTTATTTCGAACTATTCCGGACTGTCATCAACACTGGCGCTGGCACTGGCGCACACCAGTCATGCATTCACCttcttc                                                                       >  2:44396/1‑93 (MQ=255)

                       ggCATTCGCCTTTATTTCGAACTATTCCGGACTGTCATCAACACTGGCGCTGGCACTGGCGCACACCAGTCATGCATTCACCTTCTTCTCGcc                                                                  <  1:1797362/93‑1 (MQ=255)

                          aTTCGCCTTTATTTCGAACTATTCCGGACTGTCATCAACACTGGCGCTGGCACTGGCGCACACCAGTCATGCATTCACCTTCTTCTCGCCGtc                                                               >  1:3069273/1‑92 (MQ=255)

                           ttCGCCTTTATTTCGAACTATTCCGGACTGTCATCAACACTGGCGCTGGCACTGGCGCACACCAGTCATGCATTCACCTTCTTCTCGCCGTTc                                                              >  2:1795383/1‑93 (MQ=255)

                           ttCGCCTTTATTTCGAACTATTCCGGACTGTCATCAACACTGGCGCTGGCACTGGCGCACACCAGTCATGCATTCACCTTCTTCTCGCCGTTc                                                              >  2:2392689/1‑93 (MQ=255)

                            tCGCCTTTATTTCGAACTATTCCGGACTGTCATCAACACTGGCGCTGGCACTGGCGCACACCAGTCATGCATTCACCTTCTTCTCGCCGTTcc                                                             <  1:2793940/93‑1 (MQ=255)

                            tCGCCTTTATTTCGAACTATTCCGGACTGTCATCAACACTGGCGCTGGCACTGGCGCACACCAGTCATGCATTCACCTTCTTCTCGCCGTTcc                                                             >  2:3376944/1‑93 (MQ=255)

                              gCCTTTATTTCGAACTATTCCGGACTGTCATCAACACTGGCGCTGGCACTGGCGCACACCAGTCATGCATTCACCTTCTTCTCGCCGTTCCTc                                                           <  2:1133051/93‑1 (MQ=255)

                              gCCTTTATTTCGAACTATTCCGGACTGTCATCAACACTGGCGCTGGCACTGGCGCACACCAGTCATGCATTCACCTTCTTCTCGCCGTTCCTc                                                           <  2:3250221/93‑1 (MQ=255)

                              gCCTTTATTTCGAACTATTCCGGACTGTCATCAACACTGGCGCTGGCACTGGCGCACACCAGTCATGCATTCACCTTCTTCTCGCCGTTCCTc                                                           <  2:1456639/93‑1 (MQ=255)

                                cTTTATTTCGAACTATTCCGGACTGTCATCAACACTGGCGCTGGCACTGGCGCACACCAGTCATGCATTCACCTTCTTCTCGCCGTTCCTCgg                                                         >  1:4331865/1‑93 (MQ=255)

                                cTTTATTTCGAACTATTCCGGACTGTCATCAACACTGGCGCTGGCACTGGCGCACACCAGTCATGCATTCACCTTCTTCTCGCCGTTCCTCgg                                                         >  2:1776050/1‑93 (MQ=255)

                                 tttATTTCGAACTATTCCGGACTGTCATCAACACTGGCGCTGGCACTGGCGCACACCAGTCATGCATTCACCTTCTTCTCGCCGTTCCTCggc                                                        >  1:3006951/1‑93 (MQ=255)

                                 tttATTTCGAACTATTCCGGACTGTCATCAACACTGGCGCTGGCACTGGCGCACACCAGTCATGCATTCACCTTCTTCTCGCCGTTCCTCggc                                                        >  1:3924667/1‑93 (MQ=255)

                                 tttATTTCGAACTATTCCGGACTGTCATCAACACTGGCGCTGGCACTGGCGCACACCAGTCATGCATTCACCTTCTTCTCGCCGTTCCTCggc                                                        >  2:4359523/1‑93 (MQ=255)

                                 tttATTTCGAACTATTCCGGACTGTCATCAACACTGGCGCTGGCACTGGCGCACACCAGTCATGCATTCACCTTCTTCTCGCCGTTCCTCggc                                                        >  1:2219005/1‑93 (MQ=255)

                                 tttATTTCGAACTATTCCGGACTGTCATCAACACTGGCGCTGGCACTGGCGCACACCAGTCATGCATTCACCTTCTTCTCGCCGTTCCTCggc                                                        <  1:99262/93‑1 (MQ=255)

                                 tttATTTCGAACTATTCCGGACTGTCATCAACACTGGCGCTGGCACTGGCGCACACCAGTCATGCATTAACCTTCTTCTCGCCGTTCCTCggc                                                        >  2:2869849/1‑93 (MQ=255)

                                  ttATTTCGAACTATTCCGGACTGTCATCAACACTGGCGCTGGCACTGGCGCACACCAGTCATGCATTCACCTTCTTCTCGCCGTTCCTCggct                                                       >  2:1373617/1‑93 (MQ=255)

                                  ttATTTCGAACTATTCCGGACTGTCATCAACACTGGCGCTGGCACTGGCGCACACCAGTCATGCATTCACCTTCTTCTCGCCGTTCCTCggct                                                       <  1:466272/93‑1 (MQ=255)

                                     tttCGAACTATTCCGGACTGTCATCAACACTGGCGCTGGCACTGGCGCACACCAGTCATGCATTCACCTTCTTCTCGCCGTTCCTCggctggc                                                    <  2:2188702/93‑1 (MQ=255)

                                     tttCGAACTATTCCGGACTGTCATCAACACTGGCGCTGGCACTGGCGCACACCAGTCATGCATTCACCTTCTTCTCGCCGTTCCTCggctggc                                                    >  1:914867/1‑93 (MQ=255)

                                         gAACTATTCCGGACTGTCATCAACACTGGCGCTGGCACTGGCGCACACCAGTCATGCATTCACCTTCTTCTCGCCGTTCCTCGGCTGGCTggg                                                <  1:1645599/93‑1 (MQ=255)

                                         gAACTATTCCGGACTGTCATCAACACTGGCGCTGGCACTGGCGCACACCAGTCATGCATTCACCTTCTTCTCGCCGTTCCTCGGCTGGCTggg                                                <  2:442263/93‑1 (MQ=255)

                                         gAACTATTCCGGACTGTCATCAACACTGGCGCTGGCACTGGCGCACACCAGTCATGCATTCACCTTCTTCTCGCCGTTCCTCGGCTGGCTggg                                                <  2:3711603/93‑1 (MQ=255)

                                         gAACTATTCCGGACTGTCATCAACACTGGCGCTGGCACTGGCGCACACCAGTCATGCATTCACCTTCTTCTCGCCGTTCCTCGGCTGGCTggg                                                <  1:1679709/93‑1 (MQ=255)

                                           aCTATTCCGGACTGTCATCAACACTGGCGCTGGCACTGGCGCACACCAGTCATGCATTCACCTTCTTCTCGCCGTTCCTCGGCTGGCTggggg                                              >  2:2663459/1‑93 (MQ=255)

                                           aCTATTCCGGACTGTCATCAACACTGGCGCTGGCACTGGCGCACACCAGTCATGCATTCACCTTCTTCTCGCCGTTCCTCGGCTGGCTggggg                                              <  2:1334190/93‑1 (MQ=255)

                                              aTTCCGGACTGTCATCAACACTGGCGCTGGCACTGGCGCACACCAGTCATGCATTCACCTTCTTCTCGCCGTTCCTCGGCTGGCTGGGGGTAt                                           <  2:3701782/93‑1 (MQ=255)

                                               ttCCGGACTGTCATCAACACTGGCGCTGGCACTGGCGCACACCAGTCATGCATTCACCTTCTTCTCGCCGTTCCTCGGCTGGCTGGGGGTAtt                                          <  2:4559800/93‑1 (MQ=255)

                                                tCCGGACTGTCATCAACACTGGCGCTGGCACTGGCGCACACCGGTCATGCATTCACCTTCTTCTCGCCGTTCCTCGGCTGGCTGGGGGTATTc                                         >  1:3870484/1‑93 (MQ=255)

                                                 ccGGACTGTCATCAACACTGGCGCTGGCACTGGCGCACACCAGTCATGCATTCACCTTCTTCTCGCCGTTCCTCGGCTGGCTGGGGGTATTcc                                        <  1:3522574/93‑1 (MQ=255)

                                                 ccGGACTGTCATCAACACTGGCGCTGGCACTGGCGCACACCAGTCATGCATTCACCTTCTTCTCGCCGTTCCTCGGCTGGCTGGGGGTATTcc                                        <  2:2593359/93‑1 (MQ=255)

                                                  cGGACTGTCATCAACACTGGCGCTGGCACTGGCGCACACCAGTCATGCATTCCCCTTCTTCTCGCCGTTCCTCGGCTGGCTGGGGGTATTCCt                                       <  2:1625298/93‑1 (MQ=255)

                                                  cGGACTGTCATCAACACTGGCGCTGGCACTGGCGCACACCAGTCATGCATTCACCTTCTTCTCGCCGTTCCTCGGCTGGCTGGGGGTATTCCt                                       >  1:3255940/1‑93 (MQ=255)

                                                   ggACTGTCATCAACACTGGCGCTGGCACTGGCGCACACCGGTCATGCATTCACCTTCTTCTCGCCGTTCCTCGGCTGGCTGGGGGTATTCCTg                                      >  2:4010581/1‑93 (MQ=255)

                                                     acggTCATCAACACTGGCGCTGGCACTGGCGCACACCAGTCATGCATTCACCTTCTTCTCGCCGTTCCTCGGCTGGCTGGGGGTATTCCTGAc                                    <  2:2137095/90‑1 (MQ=255)

                                                      cTGTCATCAACACTGGCGCTGGCACTGGCGCACACCAGTCATGCATTCACCTTCTTCTCGCCGTTCCTCGGCTGGCTGGGGGTATTCCTGAcc                                   >  2:2095465/1‑93 (MQ=255)

                                                         tcatcaACACTGGCGCTGGCACTGGCGCACCCCAGTCATGCATTCCCCTTCTTCTCGCCGTTCCTCGGCTGGCTGGGGGTATTCCTGACCggg                                <  1:2920022/93‑1 (MQ=255)

                                                         tcatcaACACTGGCGCTGGCACTGGCGCACACCAGTCATGCATTCACCTTCTTCTCGCCGTTCCTCGGCTGGCTGGGGGGATTCCTGACCggg                                >  2:3568727/1‑93 (MQ=255)

                                                          tatcaACACTGGCGCTGGCACTGGCGCACACCAGTCATGCATTCACCTTCTTCTCGCCGGTCCTCGGCTGGCTGGGGGTATTCCTGACCggga                               >  2:981732/2‑92 (MQ=255)

                                                          catcaACACTGGCGCTGGCTCTGGCGCACACCAGTCATGCATTCACCTTCTTCTCGCCGTTCCTCGGCTGGCTGGGGGTATTCCTGACCGGGt                               <  1:585422/93‑1 (MQ=255)

                                                          catcaACACTGGCGCTGGCACTGGCGCACACCGGTCATGCATTCACCTTCTTCTCGCCGTTCCTCGGCTGGCTGGGGGTATTCCTGACCgggc                               >  1:3865647/1‑92 (MQ=255)

                                                          catcaACACTGGCGCTGGCACTGGCGCACACCAGTCATGCATTCACCTTCTTCTCGCCGTTCCTCGGCTGGCTGGGGGTATTCCTGACCGGGt                               <  2:3411449/93‑1 (MQ=255)

                                                          catcaACACTGGCGCTGGCACTGGCGCACACCAGTCATGCATTCACCTTCTTCTCGCCGTTCCTCGGCTGGCTGGGGGTATTCCTGACCGGGt                               >  1:2298017/1‑93 (MQ=255)

                                                          catcaACACTGGCGCTGGCACTGGCGCACACCAGTCATGCATTCACCTTCTTCTCGCCGTTCCTCGGCTGGCTGGGGGGATTCCTGACCGGGt                               >  1:3514510/1‑93 (MQ=255)

                                                           atcaACACTGGCGCTGGCACTGGCGCACACCAGTCATGCATTCACCTTCTTCTCGCCGTTCCTCGGCTGGCTGGGGGTATTCCTGACCGGGTc                              <  1:1780757/93‑1 (MQ=255)

                                                           atcaACACTGGCGCTGGCACTGGCGCACACCAGTCATGCATTCACCTTCTTCTCGCCGTTCCTCGGCTGGCTGGGGGTATTCCTGACCGGGTc                              >  2:1120625/1‑93 (MQ=255)

                                                           atcaACACTGGCGCTGGCACTGGCGCACACCAGTCATGCATTCACCTTCTTCTCGCCGTTCCTCGGCTGGCTGGGGGTATTCCTGACCGGGTc                              <  1:1780756/93‑1 (MQ=255)

                                                             caccacTGGCGCTGGCACTGGCGCACACCAGTCATGCATTCACCTTCTTCTCGCCGTTCCTCGGCTGGCTGGGGGTATTCCTGACCGGGTCgg                            <  2:580125/90‑1 (MQ=255)

                                                              aaCACTGGCGCTGGCACTGGCGCACACCAGTCATGCATTCACCTTCTTCTCGCCGTTCCTCGGCTGGCTGGGGGTATTCCTGACCGGGTCGGa                           >  1:134015/1‑93 (MQ=255)

                                                              aaCACTGGCGCTGGCACTGGCGCACACCAGTCATGCATTCACCTTCTTCTCGCCGTTCCTCGGCTGGCTGGGGGTATTCCTGACCGGGTCGGa                           >  1:2633260/1‑93 (MQ=255)

                                                              aaCACTGGCGCTGGCACTGGCGCACACCAGTCATGCATTCACCTTCTTCTCGCCGTTCCTCGGCTGGCTGGGGGTATTCCTGACCGGGTCGGa                           >  1:4391336/1‑93 (MQ=255)

                                                              aaCACTGGCGCTGGCACTGGCGCACACCAGTCATGCATTCACCTTCTTCTCGCCGTTCCTCGGCTGGCTGGGGGTATTCCTGACCGGGTCGGa                           >  1:1000268/1‑93 (MQ=255)

                                                               acacTGGCGCTGGCACTGGCGCACACCAGTCATGCATTCACCTTCTTCTCGCCGTTCCTCGGCTGGCTGGGGGTATTCCTGACCGGGTCGGAt                          >  2:880130/1‑93 (MQ=255)

                                                                       gcTGGCACTGGCGCACACCAGTCATGCATTCACCTTCTTCTCGCCGTTCCTCGGCTGGCTGGGGGTATTCCTGACCGGGTCGGATACCTCATc                  <  2:502890/93‑1 (MQ=255)

                                                                       gcTGGCACTGGCGCACACCAGTCATGCATTCACCTTCTTCTCGCCGTTCCTCGGCTGGCTGGGGGGATTCCTGACCGGGTCGGATACCTCATc                  >  2:681416/1‑93 (MQ=255)

                                                                        ctggcgctggcgCACACCAGTCATGCATTCACCTTCTTCTCGCCGTTCCTCGGCTGGCTGGGGGTATTCCTGACCGGGTCGGATACCTCATCt                 <  2:4218621/93‑1 (MQ=255)

                                                                         tGGCACTGGCGCACACCAGTCATGCATTCACCTTCTTCTCGCCGTTCCTCGGCTGGCTGGGGGTATTCCTGACCGGGTCGGATACCTCATCTa                >  2:779771/1‑93 (MQ=255)

                                                                         tGGCACTGGCGCACACCAGTCATGCATTCACCTTCTTCTCGCCGTTCCTCGGCTGGCTGGGGGGATTCCTGACCGGGTCGGATACCTCATCTa                >  2:1768000/1‑93 (MQ=255)

                                                                          ggCACTGGCGCACACCAGTCATGCATTCACCTTCTTCTCGCCGTTCCTCGGCTGGCTGGGGGTATTCCTGACCGGGTCGGATACCTCATCTaa               <  1:2746799/93‑1 (MQ=255)

                                                                          ggCACTGGCGCACACCAGTCATGCATTCACCTTCTTCTCGCCGTTCCTCGGCTGGCTGGGGGTATTCCTGACCGGGTCGGATACCTCATCTaa               >  1:1185276/1‑93 (MQ=255)

                                                                          ggCACTGGCGCACACCAGTCATGCATACACCTTCTTCTCGCCGTTCCTCGGCTGGCTGGGGGTATTCCTGACCGGGTCGGATACCTCATCTaa               >  1:1762873/1‑93 (MQ=255)

                                                                           gCACTGGCGCACACCAGTCATGCATTCACCTTCTTCTCGCCGTTCCTCGGCTGGCTGGGGGTATTCCTGACCGGGTCGGATACCTCATCTAAc              <  2:3474411/93‑1 (MQ=255)

                                                                               tGGCGCACCCCAGTCATGCATTCACCTTCTTCTCGCCGTTCCTCGGCTGGCTGGGGGTATTCCTGACCGGGTCGGATACCTCATCTAACGccc          <  1:1547127/93‑1 (MQ=255)

                                                                               tGGCGCACACCAGTCATGCATTCACCTTCTTCTCGCCGTTCCTCGGCTGGCTGGGGGTATTCCTGACCGGGTCGGATACCTCATCTAACGccc          <  2:2858469/93‑1 (MQ=255)

                                                                                 gcgcACACCAGTCATTCATTCCCCTTCTTCTCGCCGTTCCTCGGCTGGCTGGGGGTATTCCTGACCGGGTCGGATACCTCATCTAACGCCCTg        <  2:2200634/93‑1 (MQ=255)

                                                                                  cgcACACCAGTCATGCATTCACCTTCTTCTCGCCGTTCCTCGGCTGGCTGGGGGTATTCCTGACCGGGTCGGATACCTCATCTAAAGCCCTGt       >  1:614900/1‑93 (MQ=255)

                                                                                   gcACACCAGTCATGCATTCACCTTCTTCTCGCCGTTCCTCGGCTGGCTGGGGGTATTCCTGACCGGGTCGGATACCTCATCTAACGCCCTGtt      >  1:2793409/1‑93 (MQ=255)

                                                                                   gcACACCAGTCATGCATTCACCTTCTTCTCGCCGTTCCTCGGCTGGCTGGGGGTATTCCTGACCGGGTCGGATACCTCATCTAACGCCCTGtt      <  1:3710737/93‑1 (MQ=255)

                                                                                   gcACACCAGTCATGCATTCACCTTCTTCTCGCCGTTCCTCGGCTGGCTGGGGGTATTCCTGACCGGGTCGGATACCTCATCTAACGCCCTGtt      <  2:271090/93‑1 (MQ=255)

                                                                                   gcACACCAGTCATGCATTCACCTTCTTCTCGCCGTTCCTCGGCTGGCTGGGGGTATTCCTGACCGGGTCGGATACCTCATCTAACGCCCTGtt      <  2:2353612/93‑1 (MQ=255)

                                                                                    cacaCCAGTCATGCATTCACCTTCTTCTCGCCGTTCCTCGGCTGGCTGGGGGTATTCCTGACCGGGTCGGATACCTCATCTAACGCCCTGTTc     >  1:4121530/1‑93 (MQ=255)

                                                                                     acacCAGTCATGCATTCACCTTCTTCTCGCCGTTCCTCGGCTGGCTGGGGGTATTCCTGACCGGGTCGGATACCTCATCTAACGCCATGTTcg    >  2:3864157/1‑93 (MQ=255)

                                                                                      cacCAGTCATGCATTCACCTTCTTCTCGCCGTTCCTCGGCTGGCTGGGGGTATTCCTGACCGGGTCGGATACCTCATCTAACGCCCTGTTcgc   >  1:1504126/1‑93 (MQ=255)

                                                                                      cacCAGTCATGCATTCACCTTCTTCTCGCCGTTCCTCGGCTGGCTGGGGGTATTCCTGACCGGGTCGGATACCTCATCTAACGCCCTGTTcgc   >  1:2955067/1‑93 (MQ=255)

ATCTACTCCATCGGTATGGTGCTGGCATTCGCCTTTATTTCGAACTATTCCGGACTGTCATCAACACTGGCGCTGGCACTGGCGCACACCGGTCATGCATTCACCTTCTTCTCGCCGTTCCTCGGCTGGCTGGGGGTATTCCTGACCGGGTCGGATACCTCATCTAACGCCCTGTTCGCC  >  NC_000913/3776250‑3776429

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 20 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`