Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,812,423 T→C R101R (CGT→CGC)  b3641 → putative transcriptional regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,812,4230TC99.1% 698.0 / ‑3.9 216R101R (CGT→CGC) b3641putative transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base T (1/1);  new base C (106/108);  total (107/109)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.90e-01

Only 100 of 226 total aligned reads displayed.

ACTCGCATCAACCTGATTCTGAAAGATGAGAAAGACACCACAGCGCGCCTGCGTCTGATTGTGTTGCTGCTTCTCGGTTTTGGTGAGCGTAATCCTGGCCTGACCCGCATCCTCACTGGTCATGCGCTAATGTTTGAACAGGATCGCCTGCAAGGGCGCATCAACCAGCTGTTCGAGC  >  NC_000913/3812334‑3812511
                                                                                         |                                                                                        
 cTCGCATCAACCTGATTCTGAAAGATGAGAAAGACACCACAGCGCGCCTGCGTCTGATTGTGTTGCTGCTTCTCGGTTTTGGTGAGCGCAATc                                                                                      >  2:1258591/1‑93 (MQ=255)
 cTCGCATCAACCTGATTCTGAAAGATGAGAAAGACACCACAGCGCGCCTGCGTCTGATTGTGTTGCTGCTTCTCGGTTTTGGTGAGCGCAATc                                                                                      >  2:2760535/1‑93 (MQ=255)
  tCGCATCAACCTGATTCTGAAAGATGAGAAAGACACCACAGCGCGCCTGCGTCTGATTGTGTTGCTGCTTCTCGGTTTTGGTGAGCGCAATcc                                                                                     >  2:494609/1‑93 (MQ=255)
  tCGCATCAACCTGATTCTGAAAGATGAGAAAGACACCACAGCGCGCCTGCGTCTGATTGTGTTGCTGCTTCTCGGTTTTGGTGAGCGCAATcc                                                                                     <  2:4452873/93‑1 (MQ=255)
  tCGCATCAACCTGATTCTGAAAGATGAGAAAGACACCACAGCGCGCCTGCGTCTGATTGTGTTGCTGCTTCTCGGTTTTGGTGAGCGCAATcc                                                                                     <  2:3984718/93‑1 (MQ=255)
   cGCATCAACCTGATTCTGAAAGATGAGAAAGACACCACAGCGCGCCTGCGTCTGATTGTGTTGCTGCTTCTCGGTTTTGGTGAGCGCAATCCt                                                                                    <  1:2480188/93‑1 (MQ=255)
    ccATCAACCTGATTCTGAAAGATGAGAAAGACACCACAGCGCGCCTGCGTCTGATTGTGTTGCTGCTTCTCGGTTTTGGTGAGCGCAATCCTg                                                                                   <  1:3263231/92‑1 (MQ=255)
     cATCAACCTGATTCTGAAAGATGAGAAAGACACCACAGCGCGCCTGCGTCTGATTGTGTTGCTGCTTCTCGGTTTTGGTGAGCGCAATCCTgg                                                                                  >  1:85739/1‑93 (MQ=255)
     cATCAACCTGATTCTGAAAGATGAGAAAGACACCACAGCGCGCCTGCGTCTGATTGTGTTGCTGCTTCTCGGTTTTGGTGAGCGCAATCCTgg                                                                                  <  1:2616957/93‑1 (MQ=255)
     cATCAACCTGATACTGAAAGATGAGAAAGACACCACAGCGCGCCTGCGTCTGATTGTGTTGCTGCTTCTCGGTTTTGGTGAGAGCAATCCTgg                                                                                  >  2:4489214/1‑93 (MQ=255)
       tCAACCTGATTCTGAAAGATGAGAAAGACACCACAGCGCGCCTGCGTCTGATTGTGTTGCTGCTTCTCGGTTTTGGTGAGCGCAATCCTGGcc                                                                                <  1:2776480/93‑1 (MQ=255)
       tCAACCTGATTCTGAAAGATGAGAAAGACACCACAGCGCGCCTGCGTCTGATTGTGTTGCTGCTTCTCGGTTTTGGTGAGCGCAATCCTGGcc                                                                                >  2:4285843/1‑93 (MQ=255)
         aaCCTGATTCTGAAAGATGAGAAAGACACCACAGCGCGCCTGCGTCTGATTGTGTTGCTGCTTCTCGGTTTTGGTGAGCGCAATCCTGGCCTg                                                                              >  2:2659312/1‑93 (MQ=255)
          aCCTGATTCTGAAAGATGAGAAAGACACCACAGCGCGCCTGCGTCTGATTGTGTTGCTGCTTCTCGGTTTTGGTGAGCGCAATCCTGGCCTgc                                                                             >  2:4125378/1‑92 (MQ=255)
            cTGATTCTGAAAGATGAGAAAGACACCACAGCGCGCCTGCGTCTGATTGTGTTGCTGCTTCTCGGTTTTGGTGAGCGCAATCCTGGCCTGAcc                                                                           >  1:1028401/1‑93 (MQ=255)
            cTGATTCTGAAAGATGAGAAAGACACCACAGCGCGCCTGCGTCTGATTGTGTTGCTGCTTCTCGGTTTTGGTGAGCGCAATCCTGGCCTGAcc                                                                           <  1:4598610/93‑1 (MQ=255)
             tGATTCTGAAAGATGAGAAAGACACCACAGCGCGCCTGCGTCTGATTGTGTTGCTGCTTCTCGGTTTTGGTGAGCGCAATCCTGGCCTGAccc                                                                          >  2:3845103/1‑93 (MQ=255)
               aTTCTGAAAGATGAGAAAGACACCACAGCGCGCCTGCGTCTGATTGTGTTGCTGCTTCTCGGTTTTGGTGAGCGCAATCCTGGCCTGACCCGc                                                                        >  2:15884/1‑93 (MQ=255)
                ttCTGAAAGATGAGAAAGACACCACAGCGCGCCTGCGTCTGATTGTGTTGCTGCTTCTCGGTTTTGGTGAGCGCAATCCTGGCCTGACCCGCa                                                                       >  1:2207645/1‑93 (MQ=255)
                 tCTGAAAGATGAGAAAGACACCACAGCGCGCCTGCGTCTGATTGTGTTGCTGCTTCTCGGTTTTGGTGAGCGCAATCCTGGCCTGACCCGCAt                                                                      <  2:3803579/93‑1 (MQ=255)
                   tGAAAGATGAGAAAGACACCACAGCGCGCCTGCGTCTGATTGTGTTGCTGCTTCTCGGTTTTGGTGAGCGCAATCCTGGCCTGACCCGCATcc                                                                    <  1:1308558/93‑1 (MQ=255)
                   tGAAAGATGAGAAAGACACCACAGCGCGCCTGCGTCTGATTGTGTTGCTGCTTCTCGGTTTTGGTGAGCGCAATCCTGGCCTGACCCGCATcc                                                                    <  1:1355092/93‑1 (MQ=255)
                    gAAAGATGAGAAAGACACCACAGCGCGCCTGCGTCTGATTGTGTTGCTGCTTCTCGGTTTTGGTGAGCGCAATCCTGGCCTGACCCGCATCCt                                                                   >  2:455241/1‑93 (MQ=255)
                     aaaGATGAGAAAGACACCACAGCGCGCCTGCGTCTGATTGTGTTGCTGCTTCTCGGTTTTGGTGAGCGCAATCCTGGCCTGACCCGCATCCTc                                                                  <  2:1770851/93‑1 (MQ=255)
                      aaGATGAGAAAGACACCACAGCGCGCCTGCGTCTGATTGTGTTGCTGCTTCTCGGTTTTGGTGAGCGCAATCCTGGCCTGACCCGCATCCTCa                                                                 >  1:4037050/1‑93 (MQ=255)
                            agaAAGACACCACAGCGCGCCTGCGTCTGATTGTGTTGCTGCTTCTCGGTTTTGGTGAGCGCAATCCTGGCCTGACCCGCATCCTCACTGGTc                                                           >  1:2502346/1‑93 (MQ=255)
                            agaAAGACACCACAGCGCGCCTGCGTCTGATTGTGTTGCTGCTTCTCGGTTTTGGTGAGCGCAATCCTGGCCTGACCCGCATCCTCACTGGTc                                                           <  1:4706559/93‑1 (MQ=255)
                              aaaGACACCACAGCGCGCCTGCGTCTGATTGTGTTGCTGCTTCTCGGTTTTGGTGAGCGCAATCCTGGCCTGACCCGCATCCTCACTGGTCAt                                                         <  2:3664858/93‑1 (MQ=255)
                               aaGACACCACAGCGCGCCTGCGTCTGATTGTGTTGCTGCTTCTCGGTTTTGGTGAGCGCAATCCTGGCCTGACCCGCATCCTCACTGGTCATg                                                        <  1:1228087/93‑1 (MQ=255)
                                aGACACCACAGCGCGCCTGCGTCTTATTGTGTTGCTGCTTCTCGGCTTTGGTGAGCGCAATCCTGGCCTGACCCGCATCCTCACTGGTCATgc                                                       >  2:4683274/1‑93 (MQ=255)
                                    accacAGCGCGCCTGCGTCTGATTGTGTTGCTGCTTCTCGGTTTTGGTGAGCGCAATCCTGGCCTGACCCGCATCCTCACTGGTCATGCGCTa                                                   >  1:3471448/1‑93 (MQ=255)
                                       acaGCGCGCCTGCGTCTGATTGTGTTGCTGCTTCTCGGTTTTGGTGAGCGCAATCCTGGCCTGACCCGCATCCTCACTGGTCATGCGCTAATg                                                >  2:3566158/1‑93 (MQ=255)
                                       acaGCGCGCCTGCGTCTGATTGTGTTGCTGCTTCTCGGTTTTGGTGAGCGCAATCCTGGCCTGACCCGCATCCTCACTGGTCATGCGCTAATg                                                <  1:2123944/93‑1 (MQ=255)
                                       acaGCGCGCCTGCGTCTGATTGTGTTGCTGCTTCTCGGTTTTGGTGAGCGCAATCCTGGCCTGACCCGCATCCTCACTGGTCATGCGCTAATg                                                >  1:2563058/1‑93 (MQ=255)
                                       acaGCGCGCCTGCGTCTGATTGTGTTGCTGCTTCTCGGTTTTGGTGAGCGCAATCCTGGCCTGACCCGCATCCTCACTGGTCATGCGCTAATg                                                <  1:2387300/93‑1 (MQ=255)
                                         aGCGCGCCTGCGTCTGATTGTGTTGCTGCTTCTCGGTTTTGGTGAGCGCAATCCTGGCCTGACCCGCATCCTCACTGGTCATGCGCTAATGtt                                              <  2:2482049/93‑1 (MQ=255)
                                          gcgcgcCTGCGTCTGATTGTGTTGCTGCTTCTCGGTTTTGGTGAGCGCAATCCTGGCCTGACCCGCATCCTCACTGGTCATGCGCTAATGttt                                             <  1:1540455/93‑1 (MQ=255)
                                            gcgcCTGCGTCTGATTGTGTTGCTGCTTCTCGGTTTTGGTGAGCGCAATCCTGGCCTGACCCGCATCCTCACTGGTCATGCGCTAATGTTTGa                                           <  2:1408974/93‑1 (MQ=255)
                                            gcgcCTGCGTCTGATTGTGTTGCTGCTTCTCGGTTTTGGTGAGCGCAATCCTGGCCTGACCCGCATCCTCACTGGTCATGCGCTAATGTTTGa                                           <  1:3288168/93‑1 (MQ=255)
                                             cgcCTGCGTCTGATTGTGTTGCTGCTTCTCGGTTTTGGTGAGCGCAATCCTGGCCTGACCCGCATCCTCACTGGTCATGCGCTAATGTTTGaa                                          <  2:888911/93‑1 (MQ=255)
                                              gcCTGCGTCTGATTGTGTTGCTGCTTCTCGGTTTTGGTGAGCGCAATCCTGGCCTGACCCGCATCCTCACTGGTCATGCGCTAATGtttcacc                                         >  1:2606548/1‑89 (MQ=255)
                                              gcCTGCGTCTGATTGTGTTGCTGCTTCTCGGTTTTGGTGAGCGCAATCCTGGCCTGACCCGCATCCTCACTGGTCATGCGCTAATGTTTGAAc                                         <  2:2901021/93‑1 (MQ=255)
                                               ccTGCGTCTGATTGTGTTGCTGCTTCTCGGTTTTGGTGAGCGCAATCCTGGCCTGACCCGCATCCTCACTGGTCATGCGCTAATGTTTGAACa                                        >  2:23583/1‑93 (MQ=255)
                                               ccTGCGTCTGATTGTGTTGCTGCTTCTCGGTTTTGGTGAGCGCAATCCTGGCCTGACCCGCATCCTCACTGGTCATGCGCTAATGTTTGAACa                                        <  2:4066627/93‑1 (MQ=255)
                                                cTGCGTCTGATTGTGTTGCTGCTTCTCGGTTTTGGTGAGCGCAATCCTGGCCTGACCCGCATCCTCACTGGTCATGCGCTAATGTTTGAACAg                                       <  1:2446466/93‑1 (MQ=255)
                                                 tGCGTCTGATTGTGTTGCTGCTTCTCGGTTTTGGTGAGCGCAATCCTGGCCTGACCCGCATCCTCACTGGTCATGCGCTAATGTTTGAACAgg                                      >  2:1671959/1‑93 (MQ=255)
                                                 tGCGTCTGATTGTGTTGCTGCTTCTCGGTTTTGGTGAGCGCAATCCTGGCCTGACCCGCATCCTCACTGGTCATGCGCTAATGTTTGAACAgg                                      >  2:1173491/1‑93 (MQ=255)
                                                 tGCGTCTGATTGTGTTGCTGCTTCTCGGTTTTGGTGAGCGCAATCCTGGCCTGACCCGCATCCTCACTGGTCATGCGCTAATGTTTGAACAgg                                      >  1:692223/1‑93 (MQ=255)
                                                  gCGTCTGATTGTGTTGCTGCTTCTCGGTTTTGGTGAGCGCAATCCTGGCCTGACCCGCATCCTCACTGGTCATGCGCTAATGTTTGAACAGGa                                     <  2:375122/93‑1 (MQ=255)
                                                   cGTCTGATTGTGTTGCTGCTTCTCGGTTTTGGTGAGCGTAATCCTGGCCTGACCCGCATCCTCACTGGTCATGCGCTAATGTTTGAACAGGAt                                    <  1:3600730/93‑1 (MQ=255)
                                                   cGTCTGATTGTGTTGCTGCTTCTCGGTTTTGGTGAGCGCAATCCTGGCCTGACCCGCATCCTCACTGGTCATGCGCTAATGTTTGAACAGGAt                                    <  1:2352491/93‑1 (MQ=255)
                                                    gTCTGATTGTGTTGCTGCTTCTCGGTTTTGGTGAGCGCAATCCTGGCCTGACCCGCATCCTCACTGGTCATGCGCTAATGTTTGAACAGGATc                                   >  2:1328125/1‑93 (MQ=255)
                                                    gTCTGATTGTGTTGCTGCTTCTCGGTTTTGGTGAGCGCAATCCTGGCCTGACCCGCATCCTCACTGGTCATGCGCTAATGTTTGAACAGGATc                                   >  2:136256/1‑93 (MQ=255)
                                                     tCTGATTGTGTTGCTGCTTCTCGGTTTTGGTGAGCGCAATCCTGGCCTGACCCGCATCCTCACTGGTCATGCGCTAATGTTTGAACAGGATCg                                  >  1:2187490/1‑93 (MQ=255)
                                                      cTGATTGTGTTGCTGCTTCTCGGTTTTGGTGAGCGCAATCCTGGCCTGACCCGCATCCTCACTGGTCATGCGCTAATGTTTGAACAGGATCGc                                 <  1:2018067/93‑1 (MQ=255)
                                                      cTGATTGTGTTGCTGCTTCTCGGTTTTGGTGAGCGCAATCCTGGCCTGACCCGCATCCTCACTGGTCATGCGCTAATGTTTGAACAGGATCGc                                 >  2:2619945/1‑93 (MQ=255)
                                                       tGATTGTGTTGCTGCTTCTCGGTTTTGGTGAGCGCAATCCTGGCCTGACCCGCATCCTCACTGGTCATGCGCTAATGTTTGAACAGGATCGcc                                >  1:191959/1‑93 (MQ=255)
                                                       tGATTGTGTTGCTGCTTCTCGGTTTTGGTGAGCGCAATCCTGGCCTGACCCGCATCCTCACTGGTCATGCGCTAATGTTTGAACAGGATCGcc                                <  2:2423772/93‑1 (MQ=255)
                                                       tGATTGTGTTGCTGCTTCTCGGTTTTGGTGAGCGCAATCCTGGCCTGACCCGCATCCTCACTGGTCATGCGCTAATGTTTGAACAGGATCGcc                                >  1:4675734/1‑93 (MQ=255)
                                                        gATTGTGTTGCTGCTTCTCGGTTTTGGTGAGCGCAATCCTGGCCTGGCCCGCATCCTCACTGGTCATGCGCTAATGTTTGAACAGGATCGCCt                               >  2:2810031/1‑93 (MQ=255)
                                                        gATTGTGTTGCTGCTTCTCGGTTTTGGTGAGCGCAATCCTGGCCTGACCCGCATCCTCACTGGTCATGCGCTAATGTTTGAACAGGATCGCCt                               >  1:1083212/1‑93 (MQ=255)
                                                         aTTGTGTTGCTGCTTCTCGGTTTTGGTGAGCGCAATCCTGGCCTGACCCGCATCCTCACTGGTCATGCGCTAATGTTTGAACAGGATCGCCTg                              >  2:2020883/1‑93 (MQ=255)
                                                          ttGTGTTGCTGCTTCTCGGTTTTGGTGAGCGCAATCCTGGCCTGACCCGCATCCTCACTGGTCATGCGCTAATGTTTGAACAGGATCGCCTGc                             >  1:3813414/1‑93 (MQ=255)
                                                           tgtgTTGCTGCTTCTCGGTTTTGGTGAGCGCAATCCTGGCCTGACCCGCATCCTCACTGGTCATGCGCTAATGTTTGAACAGGATCGCCTGCa                            <  2:3434731/93‑1 (MQ=255)
                                                            gtgtTGCTGCTTCTCGGTTTTGGTGAGCGCAATCCTGGCCTGACCCGCATCCTCACTGGTCATGCGCTAATGTTTGAACAGGATCGCCTGCaa                           >  2:4021842/1‑93 (MQ=255)
                                                              gtTGCTGCTTCTCGGTTTTGGTGAGCGCAATCCTGGCCTGACCCGCATCCTCACTGGTCATGCGCTAATGTTTGAACAGGATCGCCTGCAAgg                         >  1:1889576/1‑93 (MQ=255)
                                                              gtTGCTGCTTCTCGGTTTTGGTGAGCGCAATCCTGGCCTGACCCGCATCCTCACTGGTCATGCGCTAATGTTTGAACAGGATCGCCTGCAAgg                         >  2:4211030/1‑93 (MQ=255)
                                                               ttGCTGCTTCTCGGTTTTGGTGAGCGCAATCCTGGCCTGACCCGCATCCTCACTGGTCATGCGCTAATGTTTGAACAGGATCGCCTGCAAggg                        <  1:4636937/93‑1 (MQ=255)
                                                               ttGCTGCTTCTCGGTTTTGGTGAGCGCAATCCTGGCCTGACCCGCATCCTCACTGGTCATGCGCTAATGTTTGAACAGGATCGCCTGCAAggg                        >  1:3521357/1‑93 (MQ=255)
                                                                tgctgcTTCTCGGTTTTGGTGAGCGCAATCCTGGCCTGACCCGCATCCTCACTGGTCATGCGCTAATGTTTGAACAGGATCGCCTGCAAGGgc                       >  1:277165/1‑93 (MQ=255)
                                                                 gctgctTCTCGGTTTTGGGGAGGCCAATCCGGGCCTGCCCCCCATCCTCCCTGGCAATGGCCTAATGTTTGACCAGGATCCCCGGCAAggggc                      >  2:1522434/1‑91 (MQ=255)
                                                                   tgctTCTCGGTTTTGGTGAGCGCAATCCTGGCCTGACCCGCATCCTCACTGGTCATGCGCTAATGTTTGAACAGGATCGCCTGCAAGGGCGCa                    <  1:2933272/93‑1 (MQ=255)
                                                                   tgctTCTCGGTTTTGGTGAGCGCAATCCTGGCCTGACCCGCATCCTCACTGGTCATGCGCTAATGTTTGAACAGGATCGCCTGCAAGGGCGCa                    >  2:62696/1‑93 (MQ=255)
                                                                   tgctTCTCGGTTTTGGTGAGCGCAATCCTGGCCTGACCCGCATCCTCACTGGTCATGCGCTAATGTTTGAACAGGATCGCCTGCAAGGGCGCa                    >  1:3943833/1‑93 (MQ=255)
                                                                      ttCTCGGTTTTGGTGAGCGCAATCCTGGCCTGACCCGCATCCTCACTGGTCATGCGCTAATGTTTGAACAGGATCGCCTGCAAGGGCGCATCa                 >  2:4669549/1‑93 (MQ=255)
                                                                       tctcGGTTTTGGTGAGCGCAATCCTGGCCTGACCCGCATCCTCACTGGTCATGCGCTAATGTTTGAACAGGATCGCCTGCAAGGGCGCATCaa                <  1:3094790/93‑1 (MQ=255)
                                                                        ctcGGTTTTGGTGAGCGCAATCCTGGCCTGACCCGCATCCTCACTGGTCATGCGCTGATGTTTGAACAGGATCGCCTGCAAGGGCGCATCAAc               >  2:2832290/1‑93 (MQ=255)
                                                                        ctcGGTTTTGGTGAGCGCAATCCTGGCCTGACCCGCATCCTCACTGGTCATGCGCTAATGTTTGAACAGGATCGCCTGCAAGGGCGCATCAAc               <  2:3276597/93‑1 (MQ=255)
                                                                         tcGGTTTTGGTGAGCGCAATCCTGGCCTGACCCGCATCCTCACTGGTCATGCGCTAATGTTTGAACAGGATCGCCTGCAAGGGCGCATCAAcc              <  1:4527561/93‑1 (MQ=255)
                                                                         tcGGTTTTGGTGAGCGCAATCCTGGCCTGACCCGCATCCTCACTGGTCATGCGCTAATGTTTGAACAGGATCGCCTGCAAGGGCGCATCAAcc              >  2:786898/1‑93 (MQ=255)
                                                                          cGGTTTTGGTGAGCGCAATCCTGGCCTGACCCGCATCCTCACTGGTCATGCGCTAATGTTTGAACAGGATCGCCTGCAAGGGCGCATCAACCa             <  1:3369145/93‑1 (MQ=255)
                                                                          cGGTTTTGGTGAGCGCAATCCTGGCCTGACCCGCATCCTCACTGGTCATGCGCTAATGTTTGAACAGGAGCGCCTGCAAGGGCGCATCAACCa             >  2:2578707/1‑93 (MQ=255)
                                                                           ggTTTTGGTGAGCGCAATCCTGGCCTGACCCGCATCCTCTCTGGTCATGCGCTAATGTTTGAACAGGATCGCCTGCAAGGGCGCATCAACCAg            <  2:3444425/93‑1 (MQ=255)
                                                                           ggTTTTGGTGAGCGCAATCCTGGCCTGACCCGCATCCTCACTGGTCATGCGCTAATGTTTGAACAGGATCGCCTGCAAGGGCGCATCAACCAg            >  1:4125483/1‑93 (MQ=255)
                                                                           ggTTTTGGTGAGCGCAATCCTGGCCTGACCCGCATCCTCACTGGTCATGCGCTAATGTTTGAACAGGATCGCCTGCAAGGGCGCATCAACCAg            >  2:1986570/1‑93 (MQ=255)
                                                                            gTTTTGGTGCGCGCAATCCTGGCCTGACCCGCATCCTCACTGGTCATGCGCTAATGTTTGAACAGGATCGCCTGCAAGGGAGCATCAACCAGc           <  2:2060469/93‑1 (MQ=255)
                                                                            gTTTTGGTGAGCGCAATCCTGGCCTGACCCGCATCCTCACTGGTCATGCGCTAATGTTTGAACAGGATCGCCTGCAAGGGCGCATCAACCAGc           <  1:1725590/93‑1 (MQ=255)
                                                                            gTTTTGGTGAGCGCAATCCTGGCCTGACCCGCATCCTCACTGGTCATGCGCTAATGTTTGAACAGGATCGCCTGCAAGGGCGCATCAACCAGc           <  1:998983/93‑1 (MQ=255)
                                                                            gTTTTGGTGAGCGCAATCCTGGCCTGACCCGCATCCTCACTGGTCATGCGCTAATGTTTGAACAGGATCGCCTGCAAGGGCGCATCAACCAGc           <  1:398889/93‑1 (MQ=255)
                                                                             ttttGGTGAGCGCAATCCTGGCCTGACCCGCATCCTCACTGGTCATGCGCTAATGTTTGAACAGGATCGCCTGCAAGGGCGCATCAACCAGCt          <  2:3700434/93‑1 (MQ=255)
                                                                             ttttGGTGAGCGCAATCCTGGCCTGACCCGCATCCTCACTGGTCATGCGCTAATGTTTGAACAGGATCGCCTGCAAGGGCGCATCAACCAGCt          >  1:3219673/1‑93 (MQ=255)
                                                                             ttttGGTGAGCGCAATCCTGGCCTGACCCGCATCCTCACTGGTCATGCGCTAATGTTTGAACAGGATCGCCTGCAAGGGCGCATCAACCAGCt          >  2:2415140/1‑93 (MQ=255)
                                                                              tttGGTGAGCGCAATCCTGGCCTGACCCGCATCCTCACTGGTCATGCGCTAATGTTTGAACAGGATCGCCTGCAAGGGCGCATCAACCAGCTg         <  1:2149223/93‑1 (MQ=255)
                                                                               ttGGTGAGCGCAATCCTGGCCTGACCCGCATCCTCACTGGTCATGCGCTAATGTTTGAACAGGATCGCCTGCAAGGGCGCATCAACCAGCTGt        <  1:1201339/93‑1 (MQ=255)
                                                                                tGGTGAGCGCAATCCTGGCCTGACCCGCATCCTCACTGGTCATGCGCTAATGTTTGAACAGGATCGCCTGCAAGGGCGCATCAACCAGCTGtt       <  2:2145719/93‑1 (MQ=255)
                                                                                tGGTGAGCGCAATCCTGGCCTGACCCGCATCCTCACTGGTCATGCGCTAATGTTTGAACAGGATCGCCTGCAAGGGCGCATCAACCAGCTGtt       <  1:4359908/93‑1 (MQ=255)
                                                                                   tGAGCGCAATCCTGGCCTGACCCGCATCCTCACTGGTCATGCGCTAATGTTTGAACAGGATCGCCTGCAAGGGCGCATCAACCAGCTGTTCGa    <  2:3229184/93‑1 (MQ=255)
                                                                                    gAGCGCAATCCTGGCCTGACCCGCATCCTCACTGGTCATGCGCTAATGTTTGAACAGGATCGCCTGCAAGGGCGCATCAACCAGCTGTTCGAg   <  2:2400212/93‑1 (MQ=255)
                                                                                     aGCGCAATCCTGGCCTGACCCGCATCCTCACTGGTCATGCGCTAATGTTTGAACAGGATCGCCTGCAAGGGCGCATCAACCAGCTGTTCGAGc  <  1:2206440/93‑1 (MQ=255)
                                                                                     aGCGCAATCCTGGCCTGACCCGCATCCGCACTGGTCATGCTCTAATGTTTGAACAGGATCGCCTGCAAGGGCGCATCAACCAGCTGTTCGAGc  <  2:3410119/93‑1 (MQ=255)
                                                                                         |                                                                                        
ACTCGCATCAACCTGATTCTGAAAGATGAGAAAGACACCACAGCGCGCCTGCGTCTGATTGTGTTGCTGCTTCTCGGTTTTGGTGAGCGTAATCCTGGCCTGACCCGCATCCTCACTGGTCATGCGCTAATGTTTGAACAGGATCGCCTGCAAGGGCGCATCAACCAGCTGTTCGAGC  >  NC_000913/3812334‑3812511

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑