BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	477,192	A→G	L219P (CTG→CCG)	ylaB ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	477,192	0	A	G	97.9%	751.0 / 1.2	239	L219P (CTG→CCG)	ylaB	hypothetical protein
Reads supporting (aligned to +/- strand): ref base A (3/2); new base G (112/121); total (115/124)
Fisher's exact test for biased strand distribution p-value = 6.75e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 248 total aligned reads displayed.

AACCAAATAAAGACTTGCCGATGCCAGCCTTTCTGCAACATTTTCGTTGAAGCCCATGTGATGATCGAAATATTCATCTCCGGTAAGGGCAGGATATCGTAAATGATTCCATTATTTTCGATATGCTCACCGGGTGTTTTTTGTAGCCTGGTAATAATTCCCTGAGCAATTTCATCGCT  >  NC_000913/477102‑477280

aaCCAAATAAAGACTTGCCGATGCCAGCCTTTCTGCAACATTTTCGTTGAAGCCCATGTGATGATCGAAATATTCATCTCCGGTAAGGGCggg                                                                                        <  1:411642/93‑1 (MQ=255)

aaCCAAATAAAGACTTGCCGATGCCAGCCTTTCTGCAACATTTTCGTTGAAGCCCATGTGATGATCGAAATATTCATCTCCGGTAAGGGCggg                                                                                        <  2:13919/93‑1 (MQ=255)

 aCCAAATAAAGACTTGCCGATGCCAGCCTTTCTGCAACATTTTCGTTGAAGCCCATGTGATGATCGAAATATTCATCTCCGGTAAGGGCGGGa                                                                                       >  1:4469265/1‑93 (MQ=255)

 aCCAAATAAAGACTTGCCGATGCCAGCCTTTCTGCAACATTTTCGTTGAAGCCCATGTGATGATCGAAATATTCATCTCCGGTAAGGGCGGGa                                                                                       >  1:2994212/1‑93 (MQ=255)

  ccAAATAAAGACTTGCCGATGCCAGCCTTTCTGCAACATTTTCGTTGAAGCCCATGTGATGATCGAAATATTCATCTCCGGTAAGGGCGGGat                                                                                      <  1:1247453/93‑1 (MQ=255)

  ccAAATAAAGACTTGCCGATGCCAGCCTTTCTGCAACATTTTCGTTGAAGCCCATGTGATGATCGAAATATTCATCTCCGGTAAGGGCGGGat                                                                                      <  1:4639696/93‑1 (MQ=255)

   cAAATAAAGACTTGCCGATGCCAGCCTTTCTGCAACATTTTCGTTGAAGCCCATGTGATGATCGAAATATTCATCTCCGGTAAGGGCGGGata                                                                                     <  1:3603543/93‑1 (MQ=255)

   cAAATAAAGACTTGCCGATGCCAGCCTTTCTGCAACATTTTCGTTGAAGCCCATGTGATGATCGAAATATTCATCTCCGGTAAGGGCGGGata                                                                                     >  1:4408388/1‑93 (MQ=255)

   cAAATAAAGACTTGCCGATGCCAGCCTTTCTGCAACATTTTCGTTGAAGCCCATGTGATGATCGAAATATTCATCTCCGGTAAGGGCGGGata                                                                                     <  2:314807/93‑1 (MQ=255)

   cAAATAAAGACTTGCCGATGCCAGCCTTTCTGCAACATTTTCGTTGAAGCCCATGTGATGATCGAAATATTCATCTCCGGTAAGGGCGGGata                                                                                     >  2:639931/1‑93 (MQ=255)

     aaTAAAGACTTGCCGATGCCAGCCTTTCTGCAACATTTTCGTTGAAGCCCATGTGATGATCGAAATATTCATCTCCGGTAAGGGCGGGATATc                                                                                   <  2:2112065/93‑1 (MQ=255)

        aaaGACTTGACGATGCCAGCCTTTCTGCAACATTTTCGTTGAAGCCCATGTGATGATCGAAATATTCATCTCCGGTAAGGGCGGGATATCGTa                                                                                >  2:3985483/1‑93 (MQ=255)

         aaGACTTGCCGATGCCAGCCTTTCTGCAACATTTTCGTTGAAGCCCATGTGATGATCGTAATATTCATCTCCGGTAAGGGCGGGATATCGTaa                                                                               <  2:2974224/93‑1 (MQ=255)

          aGACTTGCCGATGCCAGCCTTTCTGCAACATTTTCGTTGAAGCCCATGTGATGATCGAAATATTCATCTCCGGTAAGGGCGGGATATCGTaaa                                                                              >  1:1170293/1‑93 (MQ=255)

          aGACTTGCCGATGCCAGCCTTTCTGCAACATTTTCGTTGAAGCCCATGTGATGATCGAAATATTCATCTCCGGTAAGGGCGGGATATCGTaaa                                                                              >  1:1903696/1‑93 (MQ=255)

          aGACTTGCCGATGCCAGCCTTTCTGCAACATTTTCGTTGAAGCCCATGTGATGATCGAAATATTCATCTCCGGTAAGGGCGGGATATCGTaaa                                                                              <  1:4090851/93‑1 (MQ=255)

            aCTTGCCGATGCCAGCCTTTCTGCAACATTTTCGTTGAAGCCCATGTGATGATCGAAATATTCATCTCCGGTAAGGGCGGGATATCGTAAATg                                                                            >  1:653062/1‑93 (MQ=255)

            aCTTGCCGATGCCAGCCTTTCTGCAACATTTTCGTTGAAGCCCATGTGATGATCGAAATATTCATCTCCGGTAAGGGCGGGATATCGTAAATg                                                                            >  2:1069637/1‑93 (MQ=255)

             cTTGCCGATGCCAGCCTTTCTGCAACATTTTCGTTGAAGCCCATGTGATGATCGAAATATTCATCTCCGGTAAGGGCGGGATATCGTAAATGa                                                                           >  2:452227/1‑93 (MQ=255)

                gCCGATGCCAGCTTTTCTGCAACATTTTCGTTGAAGCCCATGTGATGATCGAAATATTCATCTCCGGTAAGGGCGGGATATCGTAAATGATTc                                                                        <  1:1916399/93‑1 (MQ=255)

                   gATGCCAGCCTTTCTGCAACATTTTCGTTGAAGCCCATGTGATGATCGAAATATTCATCTCCGGTAAGGGCGGGATATCGTAAATGATTCCat                                                                     <  1:3852765/93‑1 (MQ=255)

                   gATGCCAGCCTTTCTGCAACATTTTCGTTGAAGCCCATGTGATGATCGAAATATTCATCTCCGGTAAGGGCGGGATATCGTAAATGATTCCat                                                                     >  1:1527886/1‑93 (MQ=255)

                     tGCCAGCCTTTCTGCAACATTTTCGTTGAAGCCCATGTGATGATCGAAATATTCATCTCCGGTAAGGGCGGGATATCGTAAATGATTCCatta                                                                   >  1:705181/1‑93 (MQ=255)

                     tGCCAGCCTTTCTGCAACATTTTCGTTGAAGCCCATGTGATGATCGAAATATTCATCTCCGGTAAGGGCGGGATATCGTAAATGATTCCatta                                                                   <  1:4552940/93‑1 (MQ=255)

                       ccAGCCTTTCTGCAACATTTTCGTTGAAGCCCATGTGATGATCGAAATATTCATCTCCGGTAAGGGCGGGATATCGTAAATGATTCCattatt                                                                 >  1:226087/1‑93 (MQ=255)

                        cAGCCTTTCTGCAACATTTTCGTTGAAGCCCATGTGATGATCGAAATATTCATCTCCGGTAAGGGCGGGATATCGTAAATGATTCCATTAttt                                                                >  2:2703574/1‑93 (MQ=255)

                         aGCCTTTCTGCAACATTTTCGTTGAAGCCCATGTGATGATCGAAATATTCATCTCCGGTAAGGGCGGGATATCGTAAATGATTCCATTAtttt                                                               <  1:1740804/93‑1 (MQ=255)

                          gCCTTTCTGCAACATTTTCGTTGAAGCCCATGTGATGATCGAAATATTCATCTCCGGTAAGGGCGGGATATCGTAAATGATTCCATTAttttt                                                              >  1:585922/1‑92 (MQ=255)

                          gCCTTTCTGCAACATTTTCGTTGAAGCCCATGTGATGATCGAAATATTCATCTCCGGTAAGGGCGGGATATCGTAAATGATTCCATTATTTTc                                                              <  1:1663380/93‑1 (MQ=255)

                          gCCTTTCTGCAACATTTTCGTTGAAGCCCATGTGATGATCGAAATATTCATCTCCGGTAAGGGCGGGATATCGTAAATGATTCCATTATTTTc                                                              <  1:3691045/93‑1 (MQ=255)

                           ccTTTCTGCAACATTTTCGTTGAATCCCATGTGATTATCGAAATATTCATCTCCGGTAAGGGCGGGATATCGTAAATGATTCCATTATTTTCg                                                             <  1:1051078/93‑1 (MQ=255)

                            cTTTCTGCAACATTTTCGTTGAAGCCCATGTGATGATCGAAATATTCATCTCCGGTAAGGGCGGGATATCGTAAATGATTCCATTATTTTCGa                                                            <  2:4000919/93‑1 (MQ=255)

                             tttCTGCAACATTTTCGTTGAATCCCATGTGATGATCGAAATATTCATCTCCGGTAAGGGCGGGATATCGTAAATGATTCCATTATTTTCGat                                                           <  1:4155423/93‑1 (MQ=255)

                             tttCTGCAACATTTTCGTTGAAGCCCATGTGATGATCGAAATATTCATCTCCGGTAAGGGCGGGATATCGTAAATGATTCCATTATTTTCGat                                                           <  1:1406421/93‑1 (MQ=255)

                              ttCTGCAACATTTTCGTTGAAGCCCATGTGATGATCGAAATATTCATCTCCGGTAAGGGCGGGATATCGTAAATGATTCCATTATTTTCGata                                                          <  1:3582343/93‑1 (MQ=255)

                              ttCTGCAACATTTTCGTTGAAGCCCATGTGATGATCGAAATATTCATCTCCGGTAAGGGCGGGATATCGTAAATGATTCCATTATTTTCGata                                                          <  1:2623131/93‑1 (MQ=255)

                              ttCTGCAACATTTTCGTTGAAGCCCATGTGATGATCGAAATATTCATCTCCGGTAAGGGCGGGATATCGTAAATGATTCCATTATTTTCGata                                                          <  1:4274056/93‑1 (MQ=255)

                               tCTGCAACATTTTCGTTGAAGCCCATGTGATGATCGAAATATTCATCTCCGGTAAGGGCGGGATATCGTAAATGATTCCATTATTTTCGatat                                                         >  2:2342496/1‑93 (MQ=255)

                               tCTGCAACATTTTCGTTGAAGCCCATGTGATGATCGAAATATTCATCTCCGGTAAGGGCGGGATATCGTAAATGATTCCATTATTTTCGatat                                                         <  2:2507994/93‑1 (MQ=255)

                                cTGCAACATTTTCGTTGAAGCCCATGTGATGATCGAAATATTCATCTCCGGTAAGGGCGGGATATCGTAAATGATTCCATTATTTTCGATATg                                                        <  1:3052420/93‑1 (MQ=255)

                                cTGCAACATTTTCGTTGAAGCCCATGTGATGATCGAAATATTCATCTCCGGTAAGGGCGGGATATCGTAAATGATTCCATTATTTTCGATATg                                                        <  1:2364105/93‑1 (MQ=255)

                                  gCAACATTTTCGTTGAAGCCCATGTGATGATCGAAATATTCATCTCCGGTAAGGGCGGGATATCGTAAATGATTGCATTATTTTCGATATgtt                                                      >  1:765694/1‑91 (MQ=255)

                                  gCAACATTTTCGTTGAAGCCCATGTGATGATCGAAATATTCATCTCCGGTAAGGGCGGGATATCGTAAATGATTCCATTATTTTCGATATGCt                                                      >  1:5372/1‑93 (MQ=255)

                                  gCAACATTTTCGTTGAAGCCCATGTGATGATCGAAATATTCATCTCCGGTAAGGGCGGGATATCGTAAATGATTCCATTATTTTCGATATGCt                                                      >  1:4266601/1‑93 (MQ=255)

                                   cAACATTTTCGTTGAAGCCCATGTGATGATCGAAATATTCATCTCCGGTAAGGGCGGGATATCGTAAATGATTCCATTATTTTCGATATGCTc                                                     <  1:4734747/93‑1 (MQ=255)

                                   cAACATTTTCGTTGAAGCCCATGTGATGATCGAAATATTCATCTCCGGTAAGGGCGGGATATCGTAAATGATTCCATTATTTTCGATATGCTc                                                     <  2:1255974/93‑1 (MQ=255)

                                   cAACATTTTCGTTGAAGCCCATGTGATGATCGAAATATTCATCTCCGGTAAGGGCGGGATATCGTAAATGATTCCATTATTTTCGATATGCTc                                                     >  2:3450874/1‑93 (MQ=255)

                                   cAACATTTTCGTTGAAGCCCATGTGATGATCGAAATATTCATCTCCGGTAAGGGCGGGATATCATAAATGATTCCATTATTTTCGATATGCTc                                                     >  1:862249/1‑93 (MQ=255)

                                    aaCATTTTCGTTGAAGCCCATGTGATGATCGAAATATTCATCTCCGGTAAGGGCGGGATATCGTAAATGATTCCATTATTTTCGATATGCTCa                                                    >  1:2216990/1‑93 (MQ=255)

                                       aTTTTCGTTGAAGCCCATGTGATGATCGAAATATTCATCTCCGGTAAGGGCGAGATATCGTAAATGATTCCATTATTTTCGATATGCTCACCg                                                 >  1:2721334/1‑93 (MQ=255)

                                         tttCGTTGAAGCCGATGTGATGATCGAAATATTCATCTCGGGTAAGGGCAGGATATCGTAAATGATTCCATTATTTTCGATATGCTCACCggg                                               <  2:731148/93‑1 (MQ=255)

                                         tttCGTTGAAGCCCATGTGATGATCGAAATATTCATCTCCGGTAAGGGCGGGATATCGTAAATGATTCCATTATTTTCGATATGCTCACCggg                                               <  1:2115567/93‑1 (MQ=255)

                                         tttCGTTGAAGCCCATGTGATGATCGAAATATTCATCTCCGGTAAGGGCGGGATATCGTAAATGATTCCATTATTTTAGATATGCTCACCgag                                               >  2:2074998/1‑91 (MQ=255)

                                             gTTGAAGCCCATGTGATGATCGAAATATTCATCTCCGGTAAGGGCGGAATATCGTAAATGATTCCATTATTTTCGATATGCTCACCGGGTGtt                                           <  1:3363458/93‑1 (MQ=255)

                                                  aGCCCATGTGATGATCGAAATATTCATCTCCGGTAAGGGCGGGATATCGTAAATGATTCCATTATTTTCGATATGCTCACCGGGTGTTTTTTg                                      >  1:2875837/1‑93 (MQ=255)

                                                   gCCCATGTGATGATCGAAATATTCATCTCCGGTAAGGGCGGGATATCGTAAATGATTCCATTATTTTCGATATGCTCACCGGGTGTTTTTTGt                                     <  1:1272394/93‑1 (MQ=255)

                                                    cccATGTGATGATCGAAATATTCATCTCCGGTAAGGGCGGGATATCGTAAATGATTCCATTATTTTCGATATGCTCACCGGGTGTTTTTTGTa                                    <  1:4340051/93‑1 (MQ=255)

                                                    cccATGTGATGATCGAAATATTCATCTCCGGTAAGGGCGGGATATCGTAAATGATTCCATTATTTTCGATATGCTCACCGGGTGTTTTTTGTa                                    <  1:3939932/93‑1 (MQ=255)

                                                     ccATGTGATGATCGAAATATTCATCTCCGGTAAGGGCGGGATATCGTAAATGATTCCATTATTTTCGATATGCTCACCGGGTGTTTTTTGTAg                                   <  2:3916957/93‑1 (MQ=255)

                                                      cATGTGATGATCGAAATATTCATCTCCGGTAAGGGCGGGATATCGTAAATGATTCCATTATTTTCGATATGCTCACCGGGTGTTTTTTGTAGc                                  >  2:4042673/1‑93 (MQ=255)

                                                      cATGTGATGATCGAAATATTCATCTCCGGTAAGGGCGGGATATCGTAAATGATTCCATTATTTTCGATATGCTCACCGGGTGTTTTTTGTAGc                                  >  2:1709600/1‑93 (MQ=255)

                                                      cATGTGATGATCGAAATATTCATCTCCGGTAAGGGCGGGATATCGTAAATGATTCCATTATTTTCGATATGCTCACCGGGTGTTTTTTGTAGc                                  >  2:2650540/1‑93 (MQ=255)

                                                       aTGTGATGCTCGAAATATTCATCTCCGGTAAGGGCGGGATATCGTAAATGATTCCATTATTTTCGATATGCTCACCGGGTGTTTTTTGTAGcc                                 <  1:2819048/93‑1 (MQ=255)

                                                       aTGTGATGATCGAAATATTCATCTCCGGTAAGGGCGGGATATCGTAAATGATTCCATTATTTTCGATATGCTCACCGGGTGTTTTTTTTTGcc                                 >  2:3750828/1‑93 (MQ=255)

                                                       aTGTGATGATCGAAATATTCATCTCCGGTAAGGGCGGGATATCGTAAATGATTCCATTATTTTCGATATGCTCACCGGGTGTTTTTTGTAGcc                                 <  2:1589619/93‑1 (MQ=255)

                                                       aTGTGATGATCGAAATATTCATCTCCGGTAAGGGCGGGATATCGTAAATGATTCCATTATTTTCGATATGCTCACCGGGTGTTTTTTGTAGcc                                 <  2:2551892/93‑1 (MQ=255)

                                                        tgtgATGATCGAAATATTCATCTCCGGTAAGTGCGGGATATCGTAAATGATTCCATTATTTTCGATATGCTCACCGGGTGTTTTTTGTAGCCt                                <  1:3037200/93‑1 (MQ=255)

                                                         gtgATGATCGAAATATTCATCTCCGGTAAGGGCGGGATATCGTAAATGATTCCATTATTTTCGATATGCTCACCGGGTGTTTTTTGTAGCCTg                               >  2:2840066/1‑93 (MQ=255)

                                                          tgatgaTCGAAATATTCATCTCCGGTAAGGGCGGGATATCGTAAATGATTCCATTATTTTCGATATGCTCACCGGGTGTTTTTTGTAGCCTgg                              >  1:4598486/1‑93 (MQ=255)

                                                           gatgatCGAAATATTCATCTCCGGTAAGGGCGGGATATCGTAAATGATTCCATTATTTTCGATATGCTCACCGGGTGTTTTTTGTAGCCTGGt                             >  2:243351/1‑93 (MQ=255)

                                                           gatgatCGAAATATTCATCTCCGGTAAGGGCGGGATATCGTAAATGATTCCATTATTTTCGATATGCTCACCGGGTGTTTTTTGTAGCCTGGt                             >  1:1766631/1‑93 (MQ=255)

                                                            atgatCGAAATATTCATCTCCGGTAAGGGCGGGATATCGTAAATGATTCCATTATTTTCGCTATGCTCACCGGGTGTTTTTTGTAGCCTGGta                            >  2:837359/1‑93 (MQ=255)

                                                             tgatCGAAATATTCATCTCCGGTAAGGGCAGGATATCGTAAATGATTCCATTATTTTCGATATGCTCACCGGGTGTTTTTTGTAGCCTGGtaa                           >  2:4111767/1‑93 (MQ=255)

                                                             tgatCGAAATATTCATCTCCGGTAAGGGCAGGATATCGTAAATGATTCCATTATTTTCGATATGCTCACCGGGTGTTTTTTGTAGCCTGGtaa                           >  2:741738/1‑93 (MQ=255)

                                                              gatCGAAATATTCATCTCCGGTAAGGGCGGGATATCGTAAATGATTCCATTATTTTCGATATGCTCACCGGGTGTTTTTTGTAGCCTGGtaat                          <  2:4630914/93‑1 (MQ=255)

                                                               atCGAAATATTCATCTCCGGTAAGGGCGGGATATCGTAAATGATTCCATTATTTTCGATATGCTCACCGGGTGTTTTTTGTAGCCTGGtaata                         <  2:4723858/93‑1 (MQ=255)

                                                                tCGAAATATTCATCTCCGGTAAGGGCGGGATATCGTAAATGATTCCATTATTTTCGATATGCTCACCGGGTGTTTTTTGTAGCCTGGtaataa                        <  2:2796117/93‑1 (MQ=255)

                                                                tCGAAATATACATCTCCGGTAAGGGCGGGATATCGTAAATGATTCCATTATTTTCGATATGCTCACCGGGTGTTTTTTGTAGCCTGGtaataa                        <  1:1884093/93‑1 (MQ=255)

                                                                  gAAATATTCATCTCCGGTAAGGGCGGGATATCGTAAATGATTCCATTATTTTCGATATGCTCACCGGGTGTTTTTTGTAGCCTGGTAATAAtt                      >  1:2510129/1‑93 (MQ=255)

                                                                  gAAATATTCATCTCCGGTAAGGGCGGGATATCGTAAATGATTCCATTATTTTCGATATGCTCACCGGGTGTTTTTTGTAGCCTGGTAATAAtt                      >  2:390681/1‑93 (MQ=255)

                                                                  gAAATATTCATCTCCGGTAAGGGCGGGATATCGTAAATGATTCCATTATTTTCGATATGCTCACCGGGTGTTTTTTGTAGCCTGGTAATAAtt                      >  1:1599857/1‑93 (MQ=255)

                                                                     atatTCATCTCCGGTAAGGGCGGGATATCGTAAATGATTCCATTATTTTCGATATGCTCACCGGGTGTTTTTTGTAGCCTGGTAATAATTccc                   <  2:4473923/93‑1 (MQ=255)

                                                                      tatTCATCTCCGGTAAGGGCGGGATATCGTAAATGATTCCATTATTTTCGATATGCTCACCGGGTGTTTTTTGTAGCCTGGTAATAATTCCCt                  >  2:4604079/1‑93 (MQ=255)

                                                                       atTCATCTCCGGTAAGGGCGGGATATCGTAAATGATTCCATTATTTTCGATATGCTCACCGGGTGTTTTTTGTAGCCTGGTAATAATTCCCTg                 <  2:1999740/93‑1 (MQ=255)

                                                                       atTCATCTCCGGTAAGGGCGGGATATCGTAAATGATTCCATTATTTTCGATATGCTCACCGGGTGTTTTTTGTAGCCTGGTAATAATTCCCTg                 >  1:1464308/1‑93 (MQ=255)

                                                                        ttCATCTCCGGTAAGGGCGGGATATCGTAAATGATTCCATTATTTTCGATATGCTCACCGGGTGTTTTTTGTAGCCTGGTAATAATTCCCTGa                <  2:2424399/93‑1 (MQ=255)

                                                                        ttCATCTCCGGTAAGGGCGGGATATCGTAAATGATTCCATTATTTTCGATATGCTCACCGGGTGTTTTTTGTAGCCTGGTAATAATTCCCTGa                <  1:232844/93‑1 (MQ=255)

                                                                           aTCTCCGGTAAGGGCGGGATATCGTAAATGATTCCATTATTTTCGATATGCTCACCGGGTGTTTTTTGTAGCCTGGTAATAATTCCCTGAGCa             >  1:2037416/1‑93 (MQ=255)

                                                                           aTCTCCGGTAAGGGCGGGATATCGTAAATGATTCCATTATTTTCGATATGCTCACCGGGCGTTTTTTGTAGCCTGGTAATAATTCTCTGAGCa             >  1:1006021/1‑93 (MQ=255)

                                                                            tctcCGGTAAGGGCGGGATATCGTAAATGATTCCATTATTTTCGATATGCTCACCGGGTGTTTTTTGTAGCCTGGTAATAATTCCCTGAGCaa            <  2:888211/93‑1 (MQ=255)

                                                                            tctcCGGTAAGGGCGGGATATCGTAAATGATTCCATTATTTTCGATATGCTCACCGGGTGTTTTTTGTAGCCTGGTAATAATTCCCTGAGCaa            >  1:2780659/1‑93 (MQ=255)

                                                                             ctcCGGTAAGGGCGGGATATCGTAAATGATTCCATTATTTTCGATATGCTCACCGGGTGTTTTTTGTAGCCTGGTAATAATTCCCTGAGCAAt           <  1:166732/93‑1 (MQ=255)

                                                                              tcCGGTAAGGGCGGGATATCGTAAATGATTCCATTATTTTCGATATGCTCACCGGGTGTTTTTTGTAGCCTGGTAATAATTCCCTGAGCAAtt          >  2:192671/1‑93 (MQ=255)

                                                                                cGGTAAGGGCGGGATATCGTAAATGATTCCATTATTTTCGATATGCTCACCGGGTGTTTTTTGTAGCCTGGTAATAAATCCCTGAGCAATTTc        >  2:4207701/1‑93 (MQ=255)

                                                                                 ggTAAGGGCGGGATATCGTAAATGATTCCATTATTTTCGATATGCTCACCGGGTGTTTTTTGTAGCCTGGTAATAATTCCCTGAGCAATTTCa       <  2:2624683/93‑1 (MQ=255)

                                                                                 ggTAAGGGCGGGATATCGTAAATGATTCCATTATTTTCGATATGCTCACCGGGTGTTTTTTGTAGCCTGGTAATAATTCCCTGAGCAATTTCa       >  2:2227723/1‑93 (MQ=255)

                                                                                  gTAAGGGCGGGATATCGTAAATGATTCCATTATTTTCGATATGCTCACCGGGTGTTTTTTGTAGCCTGGTAATAATTCCCTGAGCAATTTCAt      <  2:142441/93‑1 (MQ=255)

                                                                                     aGGGCGGGATATCGTAAATGATTCCATTATTTTCGATATGCTCACCGGGTGTTTTTTGTAGCCTGGTAATAATTCCCTGAGCAATTTCATCgc   <  1:1839829/93‑1 (MQ=255)

                                                                                     aGGGCGGGATATCGTAAATGATTCCATTATTTTCGATATGCTCACCGGGTGTTTTTTGTAGCCTGGTAATAATTCCCTGAGCAATTTCATCgc   <  2:577842/93‑1 (MQ=255)

                                                                                      gggCGGGATATCGTAAATGATTCCATTATTTTCGATATGCTCACCGGGTGTTTTTTGTAGCCTGGTAATAATTCCCTGAGCAATTTCATCgct  >  1:3248691/1‑93 (MQ=255)

AACCAAATAAAGACTTGCCGATGCCAGCCTTTCTGCAACATTTTCGTTGAAGCCCATGTGATGATCGAAATATTCATCTCCGGTAAGGGCAGGATATCGTAAATGATTCCATTATTTTCGATATGCTCACCGGGTGTTTTTTGTAGCCTGGTAATAATTCCCTGAGCAATTTCATCGCT  >  NC_000913/477102‑477280

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 19 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`