Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,847,491 T→C L91L (TTA→TTG)  b3668 ← sensor histidine protein kinase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,847,4910TC98.2% 543.0 / ‑1.0 170L91L (TTA→TTG) b3668sensor histidine protein kinase
Reads supporting (aligned to +/- strand):  ref base T (2/1);  new base C (77/90);  total (79/91)
Fisher's exact test for biased strand distribution p-value = 5.98e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.27e-01

Only 100 of 174 total aligned reads displayed.

CGCCGTTAACGCCGCCCCCTGTAACAGCAAGGTGCGCCAGTCACGCTGATGGCGATAGCGCGAGATCAGCGCTACGGGCAGTAACGTCAGTAAACTACCGATCATCAATAACGGAAAATGGGTTAAACCGACCGCCTGCGTTAGCCAGTAAATCAGCAGCCACTCCGCGCCCAGCAATAC  >  NC_000913/3847401‑3847580
                                                                                          |                                                                                         
cgccgTTAACGCCGCCCCCTGTAACAGCAAGGTGCGCCAGTCACGCCGATGGCGATAGCGCGAGATCAGCGCTACGGGCAGTAACGTCAGCaa                                                                                         >  1:3372652/1‑93 (MQ=255)
   cgTTAACGCCGCCCCCTGTAACAGCAAGGTGCGCCAGTCACGCTGATGGCGATAGCGCGAGATCAGCGCTACGGGCAGTAACGTCAGCAAACt                                                                                      >  2:4577726/1‑93 (MQ=255)
    gTTAACGCCGCCCCCTGTAACAGCAAGGTGCGCCAGTCACGCTGATGGCGATAGCGCGAGATCAGCGCTACGGGCAGTAACGTCAGCAAACTa                                                                                     >  1:400926/1‑93 (MQ=255)
    gTTAACGCCGCCCCCTGTAACAGCAAGGTGCGCCAGTCACGCTGATGGCGATAGCGCGAGATCAGCGCTACGGGCAGTAACGTCAGCAAACTa                                                                                     >  2:205010/1‑93 (MQ=255)
    gTTAACGCCGCCCCCTGTAACAGCAAGGTGCGCCAGTCACGCTGATGGCGATAGCGCGAGATCAGCGCTACGGGCAGTAACGTCAGCAAACTa                                                                                     >  1:4512476/1‑93 (MQ=255)
     ttAACGCCGCCCCCTGTAACAGCAAGGTGCGCCAGTCACGCTGATGGCGATAGCGCGAGATCAGCGCTACGGGCAGTAACGTCAGCAAACTAc                                                                                    <  2:3494389/93‑1 (MQ=255)
      tAACGCCGCGCCCGGGAACAGCAAGGTGCTCCAGTCCCGCTGATGGCGATAGCGCGAGATCAGCGCTACGGGCAGTAACGTCAGCAAACTAcc                                                                                   <  2:3591684/93‑1 (MQ=255)
      tAACGCCGCCCCCTGTAACAGCAAGGTGCGCCAGTCACGCTGATGGCGATAGCGCGAGATCAGCGCTACGGGCAGTAACGTCAGCAAACTAcc                                                                                   <  2:3591683/93‑1 (MQ=255)
      tAACGCCGCCCCCTGTAACAGCAAGGTGCGCCAGTCACGCTGATGGCGATAGCGCGAGATCAGCGCTACGGGCAGTAACGTCAGCAAACTAcc                                                                                   <  1:4397185/93‑1 (MQ=255)
      tAACGCCGCCCCCTGTAACAGCAAGGTGCGCCAGTCACGCTGATGGCGATAGCGCGAGATCAGCGCTACGGGCAGTAACGTCAGCAAACTAcc                                                                                   <  2:1503923/93‑1 (MQ=255)
      tAACGCCGCCCCCTGTAACAGCAAGGTGCGCCAGTCACGCTGATGGCGATAGCGCGAGATCAGCGCTACGGGCAGTAACGTCAGCAAACTAcc                                                                                   <  1:2580217/93‑1 (MQ=255)
           ccgccCCCTGTAACAGCAAGGTGCGCCAGTCACGCTGATGGCGATAGCGCGAGATCAGCGCTACGGGCAGTAACGTCAGCAAACTACCGatca                                                                              <  2:1113775/93‑1 (MQ=255)
            cgccCCCTGTAACAGCAAGGTGCGCCAGTCACGCTGATGGCGATAGGGCGAGATCAGCGCTACGGGCAGTAACGTCAGCAAACTACCGatcat                                                                             >  2:887441/1‑93 (MQ=255)
            cgccCCCTGTAACAGCAAGGTGCGCCAGTCACGCTGATGGCGATAGCGCGAGATCAGCGCTACGGGCAGTAACGTCAGCAAACTACCGatcat                                                                             <  2:1361799/93‑1 (MQ=255)
              cccccTGTAACAGCAAGGTGCGCCAGTCACGCTGATGGCGATAGCGCGAGATCAGCGCTACGGGCAGTAACGTCAGCAAACTACCGAtcatca                                                                           >  1:2980957/1‑93 (MQ=255)
               ccccTGTAACAGCAAGGTGCGCCAGTCACGCTGATGGCGATAGCGCGAGATCAGCGCTACGGGCAGTAACGTCAGCAAACTACCGATCATCaa                                                                          >  1:3036851/1‑93 (MQ=255)
               ccccTGTAACAGCAAGGTGCGCCAGTCACGCTGATGGCGATAGCGCGAGATCAGCGCTACGGGCAGTAAAGTCAGCAAACTACCGATCATCaa                                                                          <  1:4719273/93‑1 (MQ=255)
                cccTGTAACAGCAAGGTGCGCCAGTCACGCTGATGGCGATAGCGCGAGATCAGCGCTACGGGCAGTAACGTCAGCAAACTACCGATCATCaaa                                                                         >  1:965155/1‑92 (MQ=255)
                cccTGTAACAGCAAGGTGCGCCAGTCACGCTGATGGCGATAGCGCGAGATCAGCGCTACGGGCAGTAACGTCAGCAAACTACCGATCATCAAt                                                                         >  1:2462104/1‑93 (MQ=255)
                 ccTGTAACAGCAAGGTGCGCCAGTCACGCTGATGGCGATAGCGCGAGATCAGCGCTACGGGCAGTAACGTCAGCAAACTACCGATCATCAATa                                                                        >  1:456541/1‑93 (MQ=255)
                 ccTGTAACAGCAAGGTGCGCCAGTCACGCTGATGGCGATAGCGCGAGATCAGCGCTACGGGCAGTAACGTCAGCAAACTACCGATCATCAATa                                                                        <  1:4345003/93‑1 (MQ=255)
                 ccTGTAACAGCAAGGTGCGCCAGTCACGCTGATGGCGATAGCGCGAGATCAGCGCTACGGGCAGTAACGTCAGCAAACTACCGATCATCAATa                                                                        <  2:2686145/93‑1 (MQ=255)
                  cTGTAACAGCAAGGTGCGCCAGTCACGCTGATGGCGATAGCGCGAGATCAGCGCTACGGGCAGTAACGTCAGCAAACTACCGATCATCAATaa                                                                       <  2:178269/93‑1 (MQ=255)
                   tGTAACAGCAAGGTGCGCCAGTCACGCTGATGGCGATAGCGCGAGATCAGCGCTACGGGCAGTAACGTCAGCAAACTACCGATCATAAATAAc                                                                      >  1:3459029/1‑93 (MQ=255)
                     tAACAGCAGGGTGCGCCAGTCACGCTGATGGCGATAGCGCGAGATCAGCGCTACGGGCAGTAACGTCAGCAAACTACCGATCATCAATAACgg                                                                    <  1:4205445/93‑1 (MQ=255)
                     tAACAGCAAGGTGCGCCAGTCACGCTGATGGCGATAGCGCGAGATCAGCGCTACGGGCAGTAACGTCAGCAAACTACCGATCATCAATAACgg                                                                    <  1:281177/93‑1 (MQ=255)
                       aCAGCAAGGTGCGCCAGTCACGCTGATGGCGATAGCGCGAGATCAGCGCTACGGGCAGTAACGTCAGCAAACTACCGATCATCAATAACGGaa                                                                  >  1:723499/1‑93 (MQ=255)
                         aGCAAGGTGCGCCAGTCACGCTGATGGCGATAGCGCGAGATCAGCGCTACGGGCAGTAACGTCAGCAAACTACCGATCATCAATAACGGaaaa                                                                <  2:103934/93‑1 (MQ=255)
                         aGCAAGGTGCGCCAGTCACGCTGATGGCGATAGCGCGAGATCAGCGCTACGGGCAGTAACGTCAGCAAACTACCGATCATCAATAACGGaaaa                                                                <  1:234885/93‑1 (MQ=255)
                         aGCAAGGTGCGCCAGTCACGCTGATGGCGATAGCGCGAGATCAGCGCTACGGGCAGTAACGTCAGCAAACTACCGATCATCAATAACGGaaaa                                                                >  1:1902242/1‑93 (MQ=255)
                          gCAAGGTGCGCCAGTCACGCTGATGGCGATAGCGCGAGATCAGCGCTACGGGCAGTAACGTCAGCAAACTACCGATCATCAATAACGGAAAAt                                                               <  2:2719813/93‑1 (MQ=255)
                          gCAAGGTGCGCCAGTCACGCTGATGGCGATAGCGCGAGATCAGCGCTACGGGCAGTAACGTCAGCAAACTACCGATCATCAATAACGGAAAAt                                                               <  1:3779849/93‑1 (MQ=255)
                          gCAAGGTGCGCCAGTCACGCTGATGGCGATAGCGCGAGATCAGCGCTACGGGCAGTAACGTCAGCAAACTACCGATCATCAATAACGGAAAAt                                                               >  2:4718374/1‑93 (MQ=255)
                           cAAGGTGCGCTAGTCACGCTGATGGCGATAGCGCGAGATCAGCGCTACGGGCAGTAACGTCAGCAAACTACCGATCATCAATAACGGAAAATg                                                              <  2:1207265/93‑1 (MQ=255)
                            aaGGTGCGCTAGTCACGCTGATGGCGATAGCGCGAGATCAGCGCTACGGGCAGTAACGTCAGCAAACTACCGATCATCAATAACGGAAAATgg                                                             <  2:1081996/93‑1 (MQ=255)
                             aGGTGCGCCAGTCACGCTGATGGCGATAGCGCGAGATCAGCGCTACGGGCAGTAACGTCAGCAAACTACCGATCATCAATAACGGAAAATggg                                                            <  2:3149827/93‑1 (MQ=255)
                              ggTGCGCCAGTCACGCTGATGGCGATAGCGCGAGATCAGCGCTACGGGCAGTAACGTCAGCAAACTACCGATCATCAATAACGGAAAATGGGt                                                           <  1:25384/93‑1 (MQ=255)
                               gTGCGCCAGTCACGCTGATGGCGATAGCGCGAGATCAGCGCTACGGGCAGTAACGTCAGCAAACTACCGATCATCAATAACGGAAAATGGGtt                                                          >  2:4087000/1‑93 (MQ=255)
                               gTGCGCCAGTCACGCTGATGGCGATAGCGCGAGATCAGCGCTACGGGCAGTAACGTCAGCAAACTACCGATCATCAATAACGGAAAATGGGtt                                                          <  1:140267/93‑1 (MQ=255)
                               gTGCGCCAGTCACGCTGATGGCGATAGCGCGAGATCAGCGCTACGGGCAGTAACGTCAGCAAACTACCGATCATCAATAACGGAAAATGGGtt                                                          >  2:2183493/1‑93 (MQ=255)
                               gTGCGCCAGTCACGCTGATGGCGATAGCACGAGATCAGCGCTACGGGCAGTAACGTCAGCAAACTACCGATCATCAATAACGGAAAATggggt                                                          >  2:1838125/1‑91 (MQ=255)
                                 gcgcCAGTCACGCTGATGGCGATAGCGCGAGATCAGCGCTACGGGCAGTAACGTCAGCAAACTACCGATCATCAATAACGGAAAATGGGTTaa                                                        <  2:4310894/93‑1 (MQ=255)
                                 gcgcCAGTCACGCTGATGGCGATAGCGCGAGATCAGCGCTACGGGCAGTAACGTCAGCAAACTACCGATCATCAATAACGGAAAATGGGTTaa                                                        <  1:1494232/93‑1 (MQ=255)
                                   gcCAGTCACGCTGATGGCGATAGCGCGAGATCAGCGCTACGGGCAGTAACGTCAGCAAACTACCGATCATCAATAACGGAAAATGGGTTAAac                                                      <  2:4285664/93‑1 (MQ=255)
                                    ccAGTCACGCTGATGGCGATAGCGCGAGATCAGCGCTACGGGCAGTAACGTCAGCAAACTACCGATCATCAATAACGGAAAATGGGTTAAacc                                                     >  1:3161021/1‑93 (MQ=255)
                                    ccAGACACGCTGATGGCGATAGCGCGAGATCAGCGCTACGGGCAGTAACGTCAGCAAACTACCGATCATCAATAACGGAAAAGTGGTTAAccc                                                     >  2:1416139/1‑93 (MQ=255)
                                     cAGTCACGCTGATGGCGATAGCGCGAGATCAGCGCTACGGGCAGTAACGTCAGCAAACTACCGATCATCAATAACGGAAAATGGGTTAAaccg                                                    >  2:1244211/1‑93 (MQ=255)
                                      aGTCACGCTGATGGCGATAGCGCGAGATCAGCGCTACGGGCAGTAACGTCAGCAAACTACCGATCATCAATAACGGAAAATGGGTTAAaccga                                                   >  1:4518326/1‑93 (MQ=255)
                                        tCACGCTGATGGCGATAGCGCGAGATCAGCGCTACGGGCAGTAACGTCAGCAAACTACCGATCATCAATAACGGAAAATGGGTTAAaccgacc                                                 <  1:1679194/93‑1 (MQ=255)
                                        tCACGCTGATGGCGATAGCGCGAGATCAGCGCTACGGGCAGTAACGTCAGCAAACTACCGATCATCAATAACGGAAAATGGGTTAAaccgacc                                                 <  1:2319602/93‑1 (MQ=255)
                                         cACGCTGATGGCGATAGCGCGAGATCAGCGCTACGGGCAGTAACGTCAGCAAACTACCGATCATCAATAACGGAAAATGGGTTAAaccgaccg                                                >  1:2098003/1‑93 (MQ=255)
                                          aCGCTGATGGCGATAGCGCGAGATCAGCGCTACGGGCAGTAACGTCAGCAAACTACCGATCATCAATAACGGAAAATGGGTTAAACCGACCGc                                               <  2:162671/93‑1 (MQ=255)
                                          aCGCTGATGGCGATAGCGCGAGATCAGCGCTACGGGCAGTAACGTCAGCAAACTACCGATCATCAATAACGGAAAATGGGTTAAACCGACCGc                                               >  2:1647507/1‑93 (MQ=255)
                                           ctcTGATGGCTATAGCTCGAGATCAGCGCTACGGGCAGTAACGTCTGCATACTACCGATCATCAATAACGGAATATGGGTTAAACCTACCGcc                                              >  2:4223345/3‑93 (MQ=255)
                                           cGCTGATGGCGATAGCGCGAGATCAGCGCTACGGGCAGTAACGTCAGCAAACTACCGATCATCAATAACGGAAAATGGGTTAAACCGACCGcc                                              <  2:1600395/93‑1 (MQ=255)
                                           cGCTGATGGCGATAGCGCGAGATCAGCGCTACGGGCAGTAACGTCAGCAAACTACCGATCATCAATAACGGAAAATGGGTTAAACCGACCGcc                                              <  2:1459412/93‑1 (MQ=255)
                                           cGCTGATGGCGATAGCGCGAGATCAGCGCTACGGGCAGTAACGTCAGCAAACTACCGATCATCAATAACGGAAAATGGGTTAAACCGACCGcc                                              <  2:2018545/93‑1 (MQ=255)
                                             cTGATGGCGATAGCGCGAGATCAGCGCTACGGGCAGTAACGTCAGCAAACTACCGATCATCAATAACGGAAAATGGGTTAAACCGACCGCCTg                                            <  1:2819662/93‑1 (MQ=255)
                                               gATGGCGATAGCGCGAGATCAGCGCTACGGGCAGTAACGTCAGCAAACTACCGATCATCAATAACGGAAAATGGGTTAAACCGACCGCCTGCg                                          >  1:1629306/1‑93 (MQ=255)
                                               gATGGCGATAGCGCGAGATCAGCGCTACGGGCAGTAACGTCAGCAAACTACCGATCATCAATAACGGAAAATGGGTTAAACCGACCGCCTGCg                                          >  2:2962932/1‑93 (MQ=255)
                                               gATGGCGATAGCGCGAGATCAGCGCTACGGGCAGTAACGTCAGCAAACTACCGATCATCAATAACGGAAAATGGGTTAAACCGACCGCCTGCg                                          <  1:827052/93‑1 (MQ=255)
                                                aTGGCGATAGCGCGAGATCAGCGCTACGGGCAGTAACGTCAGCAAACTACCGATCATCAATAACGGAAAATGGGTTAAACCGACCGCCTGCGt                                         >  1:3114317/1‑93 (MQ=255)
                                                aTGGCGATAGCGCGAGATCAGCGCTACGGGCAGTAACGTCAGCAAACTACCGATCATCAATAACGGAAAATGGGTTAAACCGACCGCCTGCGt                                         <  1:3626029/93‑1 (MQ=255)
                                                aTGGCGATAGCGCGAGATCAGCGCTACGGGCAGTAACGTCAGCAAACTACCGATCATCAATAACGGAAAATGGGTTAAACCGACCGCCTGCGt                                         <  2:3264163/93‑1 (MQ=255)
                                                 tGGCGATAGCGCGAGATCAGCGCTACGGGCAGTAACGTCAGCAAACTACCGATCATCAATAACGGAAAATGGGTTAAACCGACCGCCTGCGtt                                        <  1:831685/93‑1 (MQ=255)
                                                 tGGCGATAGCGCGAGATCAGCGCTACGGGCAGTAACGTCAGCAAACTACCGATCATCAATAACGGAAAATGGGTTAAACCGACCGCCTGCGtt                                        <  2:3842753/93‑1 (MQ=255)
                                                     gATAGCGCGAGATCAGCGCTACGGGCAGTAACGTCAGCAAACTACCGATCATCAATAACGGAAAATGGGTTAAACCGACCGCCTGCGTTAGcc                                    <  1:2646157/93‑1 (MQ=255)
                                                     gATAGCGCGAGATCAGCGCTACGGGCAGTAACGTCAGCAAACTACCGATCATCAATAACGGAAAATGGGTTAAACCGACCGCCTGCGTTAGcc                                    >  1:330757/1‑93 (MQ=255)
                                                      aTAGCGCGAGATCAGCGCTACGGGCAGTAACGTCAGCAAACTACCGATCATCAATAACGGAAAATGGGTTAAACCGACCGCCTGCGTTAGCCa                                   <  2:4049333/93‑1 (MQ=255)
                                                      aTAGCGCGAGATCAGCGCTACGGGCAGTAACGTCAGCAAACTACCGATCATCAATAACGGAAAATGGGTTAAACCGACCGCCTGCGTTAGCCa                                   >  1:2567202/1‑93 (MQ=255)
                                                      aTAGCGCGAGATCAGCGCTACGGGCAGTAACGTCAGCAAACTACCGATCATCAATAAAGGAAAATGGGTTAAACCGACCGCCTGCGTTAGCCa                                   <  1:851906/93‑1 (MQ=255)
                                                         gcgcGAGATCAGCGCTACGGGCAGTAACGTCAGTAAACTACCGATCATCAATAACGGAAAATGGGTTAAACCGACCGCCTGCGTTAGCCAGTa                                <  2:3459054/93‑1 (MQ=255)
                                                          cgcgAGATCAGCGCTACGGGCAGTAACGTCAGCAAACTACCGATCATCAATAACGGAAAATGGGTTAAACCGACCGCCTGCGTTAGCCAGTaa                               <  1:806767/93‑1 (MQ=255)
                                                           gcgAGATCAGCGCTACGGGCAGTAACGTCAGCAAACTACCGATCATCAATAACGGAAAATGGGTTAAACCGACCGCCTGCGTTAGCCAGTaaa                              >  1:2909775/1‑93 (MQ=255)
                                                            cgAGATCAGCGCTACGGGCAGTAACGTCAGCAAACTACCGATCATCAATAACGGAAAATGGGTTAAACCGACCGCCTGCGTTAGCCAGTAAAt                             <  1:1609472/93‑1 (MQ=255)
                                                              agaTCAGCGCTACGGGCAGTAACGTCAGCAAACTACCGATCATCAATAACGGAAAATGGGTTAAACCGACCGCCTGCGTTAGCCAGTAAATca                           <  2:491296/93‑1 (MQ=255)
                                                               gaTCAGCGCTACGGGCAGTAACGTCAGCAAACTACCGATCATCAATAACGGAAAATGGGTTAAACCGACCGCCTGCGTTAGCCAGTAAATcag                          >  2:655902/1‑93 (MQ=255)
                                                                 tCAGCGCTACGGGCAGTAACGTCAGCAAACTACCGATCATCAATAACGGAAAATGGGTTAAACCGACCGCCTGCGTTAGCCAGTAAATcagca                        >  1:3166436/1‑93 (MQ=255)
                                                                 tCAGCGCTACGGGCAGTAACGTCAGCAAACTACCGATCATCAATAACGGAAAATGGGTTAAACCGACCGCCTGCGTTAGCCAGTAAATcagca                        >  1:4661469/1‑93 (MQ=255)
                                                                  cAGCGCTACGGGCAGTAACGTCAGCAAACTACCGATCATCAATAACGGAAAATGGGTTAAACCGACCGCCTGCGTTAGCCAGTAAATcagcag                       >  1:2863877/1‑93 (MQ=255)
                                                                   aGCGCTACGGGCAGTAACGTCAGCAAACTACCGATCATCAATAACGGAAAATGGGTTAAACCGACCGCCTGCGTTAGCCAGTAAATCagcagc                      >  1:2786377/1‑93 (MQ=255)
                                                                    gcgcTACGGGCAGTAACGTCAGCAAACTACCGATCATCAATAACGGAAAATGGGTTAAACCGACCGCCTGCGTTAGCCAGTAAATCAGCAGcc                     <  2:4459964/93‑1 (MQ=255)
                                                                        tACGGGCAGTAACGTCAGCAAACTACCGATCATCAATAACGGAAAATGGGTTAAACCGACCGCCTGCGTTAGCCAGTAAATCAGCAGCCACTc                 <  2:174462/93‑1 (MQ=255)
                                                                        tACGGGCAGTAACGTCAGCAAACTACCGATCATCAATAACGGAAAATGGGTTAAACCGACCGCCTGCGTTAGCCAGTAAATCAGCAGCCACTc                 <  1:2174472/93‑1 (MQ=255)
                                                                        tACGGGCAGTAACGTCAGCAAACTACCGATCATCAATAACGGAAAATGGGTTAAACCGACCGCCTGCGTTAGCCAGTAAATCAGCAGCCACTc                 >  2:2539314/1‑93 (MQ=255)
                                                                         aCGGGCAGTAACGTCAGCAAACTACCGATCATCAATAACGGAAAATGGGTTAAACCGACCGCCTGCGTTAGCCAGTAAATCAGCAGCCACTcc                <  2:3424463/93‑1 (MQ=255)
                                                                         aCGGGCAGTAACGTCAGCAAACTACCGATCATCAATAACGGAAAATGGGTTAAAACGACCGCCTGCGTTAGCAAGTAAATCAGCAGCAACTca                >  1:607201/1‑92 (MQ=255)
                                                                          cGGGCAGTAACGTCAGCAAACTACCGATCATCAATAACGGAAAATGGGTTAAACCGACCGCCTGCGTTAGCCAGTAAATCAGCAGCCACTCcg               >  2:1891163/1‑93 (MQ=255)
                                                                           gggCAGTAACGTCAGCAAACTACCGATCATCAATAACGGAAAATGGGTTAAACCGACCGCCTGCGTTAGCCAGTAAATCAGCAGCCACTCcgc              <  2:2205470/93‑1 (MQ=255)
                                                                           gggCAGTAACGTCAGCAAACTACCGATCATCAATAACGGAAAATGGGTTAAACCGACCGCCTGCGTTAGCCAGTAAATCAGCAGCCACTCcgc              >  2:2478846/1‑93 (MQ=255)
                                                                             gCAGTAACGTCAGCAAACTACCGATCATCAATAACGGAAAATGGGTTAAACCGACCGCCTGCGTTAGCCAGTAAATCAGCACCCACTCTgcgc            >  1:1100285/1‑93 (MQ=255)
                                                                                gTAACGTCAGCAAACTACCGATCATCAATAACGGAAAATGGGTTAAACCGACCGCCTGCGTTAGCCAGTAAATCAGCAGCCACTCCGCGCCCa         <  2:146527/93‑1 (MQ=255)
                                                                                gTAACGTCAGCAAACTACCGATCATCAATAACGGAAAATGGGTTAAACCGACCGCCTGCGTTAGCCAGTAAATCAGCAGCCACTCCGCGCCCa         <  2:292246/93‑1 (MQ=255)
                                                                                 tAACGTCAGCAAACTACCGATCATCAATAACGGAAAATGGGTTAAACCGACCGCCTGCGTTAGCCAGTAAATCAGCAGCCACTCCGCGCCCAg        <  2:2385604/93‑1 (MQ=255)
                                                                                  aaCGTCAGCAAACTACCGATCATCAATAACGGAAAATGGGTTAAACCGACCGCCTGCGTTAGCCAGTAAATCAGCAGCCACTCCGCGCCCAGc       >  1:1524798/1‑93 (MQ=255)
                                                                                     gTCAGCAAACTACCGATCATCAATAACGGAAAATGGGTTAAACCGACCGCCTGCGTTAGCCAGTAAATCAGCAGCCACTCCGCGCCCAGCAAt    <  2:409053/93‑1 (MQ=255)
                                                                                      tCAGCAAACTACCGATCATCAATAACGGAAAATGGGTTAAACCGACCGCCTGCGTTAGCCAGTAAATCAGCAGCCACTCCGCGCCCAGCAATa   >  1:1230743/1‑93 (MQ=255)
                                                                                       cAGCAAACTACCGATCATCAATAACGGAAAATGGGTTAAACCGACCGCCTGCGTTAGCCAGTAAATCAGCAGCCACTCCGCGCCCAGCAATAc  <  2:4137182/93‑1 (MQ=255)
                                                                                       cAGCAAACTACCGATCATCAATAACGGAAAATGGGTTAAACCGACCGCCTGCGTTAGCCAGTAAATCAGCAGCCACTCCGCGCCCAGCAATAc  <  2:3060532/93‑1 (MQ=255)
                                                                                       cAGCAAACTACCGATCATCAATAACGGAAAATGGGTTAAACCGACCGCCTGCGTTAGCCAGTAAATCAGCAGCCACTCCGCGCCCAGCAATAc  >  1:3460415/1‑93 (MQ=255)
                                                                                          |                                                                                         
CGCCGTTAACGCCGCCCCCTGTAACAGCAAGGTGCGCCAGTCACGCTGATGGCGATAGCGCGAGATCAGCGCTACGGGCAGTAACGTCAGTAAACTACCGATCATCAATAACGGAAAATGGGTTAAACCGACCGCCTGCGTTAGCCAGTAAATCAGCAGCCACTCCGCGCCCAGCAATAC  >  NC_000913/3847401‑3847580

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 19 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑