Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,734,264 2 bp→GC coding (542‑543/825 nt) bax ← putative ATP‑binding protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,734,2640CG100.0% 878.4 / NA 265S181C (TCG→TGC) baxputative ATP‑binding protein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base G (148/117);  total (148/117)
*NC_0009133,734,2650GC99.2% 883.7 / ‑4.8 266S181C (TCG→TGC) baxputative ATP‑binding protein
Reads supporting (aligned to +/- strand):  ref base G (0/2);  new base C (148/116);  total (148/118)
Fisher's exact test for biased strand distribution p-value = 1.96e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 271 total aligned reads displayed.

GACATAGGCGCTCACCGATTCTTTGACAGAACTAAACTGTGAGTACCCTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATCGATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCTTCGACGTTCCCCAACCGCTTTCTGCTGCAGCCATCGTCGCCACCATA  >  NC_000913/3734177‑3734351
                                                                                       ||                                                                                      
gACATAGGCGCTCACCGATTCTTTGACAGAACTAAACTGTGAGTACCCTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCAttt                                                                                    >  2:1747106/1‑93 (MQ=255)
  cATAGGCGCTCACCGATTCTTTGACAGAACTAAACTGTGAGTACCCTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCa                                                                                  <  1:1337531/93‑1 (MQ=255)
   aTAGGCGCTCACCGATTCTTTGACAGAACTAAACTGTGAGTACCCTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCAt                                                                                 >  1:3831910/1‑93 (MQ=255)
   aTAGGCGCTCACCGATTCTTTGACAGAACTAAACTGTGAGTACCCTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCAt                                                                                 <  2:705613/93‑1 (MQ=255)
    tAGGCGCTCACCGATTCTTTGACAGAACTAAACTGTGAGTACCCTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATg                                                                                <  1:2517685/93‑1 (MQ=255)
    tAGGCGCTCACCGATTCTTTGACAGAACTAAACTGTGAGTACCCTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATg                                                                                >  2:170871/1‑93 (MQ=255)
    tAGGCGCTCACCGATTCTTTGACAGAACTAAACTGTGAGTACCCTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATg                                                                                <  2:2550219/93‑1 (MQ=255)
      ggCGCTCACCGATTCTTTGACAGAACTAAACTGTGAGTACCCTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGcc                                                                              >  2:2758411/1‑93 (MQ=255)
        cgcTCACCGATTCTTTGACAGAACTAAACTGTGAGTACCCTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGa                                                                            <  1:4172850/93‑1 (MQ=255)
            cACCGATTCTTTGACAGAACTAAACTGTGAGTACCCTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAg                                                                        >  1:2338770/1‑93 (MQ=255)
             aCCGATTCTTTGACAGAACTAAACTGTGAGTACCCTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAgg                                                                       >  2:1493353/1‑93 (MQ=255)
             aCCGATTCTTTGACAGAACTAAACTGTGAGTACCCTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAgg                                                                       >  2:1051404/1‑93 (MQ=255)
             aCCGATTCTTTGACAGAACTAAACTGTGAGTACCCTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAgg                                                                       <  1:4731030/93‑1 (MQ=255)
              ccGATTCTTTGACAGAACTAAACTGTGAGTACCCTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGgt                                                                      >  1:1199293/1‑93 (MQ=255)
              ccGATTCTTTGACAGAACTAAACTGTGAGTACCCTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGgt                                                                      >  1:3271519/1‑93 (MQ=255)
               cGATTCTTTGACAGAACTAAACTGTGAGTACCCTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGgtt                                                                     <  2:3997882/93‑1 (MQ=255)
               cGATTCTTTGACAGAACTAAACTGTGAGTACCCTTTCACTTTACCCGGCGCATTGGTACAACGTCCTTTCATGCACTGCAAGCCGAACAGgtg                                                                     >  2:4208357/1‑92 (MQ=255)
                gATTCTTTGACAGAACTAAACTGTGAGTACCCTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGgttg                                                                    >  1:2987836/1‑93 (MQ=255)
                gATTCTTTGACAGAACTAAACTGTGAGTACCCTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGgttg                                                                    >  1:1051248/1‑93 (MQ=255)
                 aTTCTTTGACAGAACTAAACTGTGAGTACCCTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGgttgt                                                                   <  1:3347367/93‑1 (MQ=255)
                  ttCTTTGACAGAACTAAACTGTGAGTACCCTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGgttgtt                                                                  <  2:3305389/93‑1 (MQ=255)
                  ttCTTTGACAGAACTAAACTGTGAGTACCCTTTCACTTTACCTGGAGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGgttgtg                                                                  >  1:1865737/1‑92 (MQ=255)
                  ttCTGTGACAGAACTAAACTGTGAGTACCCTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGgttgtt                                                                  <  1:4404421/93‑1 (MQ=255)
                     tttGACAGAACTAAACTGTGAGTACCCTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGgttgttgtt                                                               >  2:3410943/1‑93 (MQ=255)
                      ttGACAGAACTAAACTGTGAGTACCCTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGgttgttgttg                                                              <  1:1368365/93‑1 (MQ=255)
                      ttGACAGAACTAAACTGTGAGTACCCTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGgttgttgttg                                                              >  2:4638351/1‑93 (MQ=255)
                      ttGACAGAACTAAACTGTGAGTACCCTTTCACTTTACC‑GGGGCCTTGGGACAACGTCCTTTCCTTCATTTCATGCCGAACCGgttggtgttgt                                                             >  2:1048616/1‑93 (MQ=255)
                       tGACAGAACTAAACTGTGAGTACCCTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGgttgttgttgt                                                             <  1:840969/93‑1 (MQ=255)
                       tGACAGAACTAAACTGTGAGTACCCTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGgttgttgttgt                                                             >  1:276603/1‑93 (MQ=255)
                        gACAGAACTAAACTGTGAGTACCCTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGgttgttgttgtt                                                            >  2:3549507/1‑93 (MQ=255)
                        gACAGAACTAAACTGTGAGTACCCTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGgttgttgttgtt                                                            <  2:496840/93‑1 (MQ=255)
                        gACAGAACTAAACTGTGAGTACCCTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGgttgttgttgtt                                                            >  1:2247682/1‑93 (MQ=255)
                         aCAGAACTAAACTGTGAGTACCCTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGttgttgttgttg                                                           >  1:4645833/1‑93 (MQ=255)
                          cAGAACTAAACTGTGAGTACCCTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTgc                                                          >  1:1156794/1‑93 (MQ=255)
                           aGAACTAAACTGTGAGTACCCTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTgcg                                                         >  1:2886452/1‑93 (MQ=255)
                           aGAACTAAACTGTGAGTACCCTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTgcg                                                         >  1:3602995/1‑93 (MQ=255)
                           aGAACTAAACTGTGAGTACCCTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTgcg                                                         >  2:2470497/1‑93 (MQ=255)
                            gAACTAAACTGTGAGTACCCTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTgcgc                                                        <  1:3203664/93‑1 (MQ=255)
                            gAACTAAACTGTGAGTACCCTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTgcgc                                                        >  2:320598/1‑93 (MQ=255)
                            gAACTAAACTGTGAGTACCCTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTgcgc                                                        >  2:1680499/1‑93 (MQ=255)
                            gAACTAAACTGTGAGTACCCTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTgcgc                                                        >  2:1860648/1‑93 (MQ=255)
                             aaCTAAACTGTGAGTACCCTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTgcgcg                                                       <  1:13791/93‑1 (MQ=255)
                              aCTAAACTGTGAGTACCCTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGTTTGTTGTTGTTgcgcgc                                                      >  2:2233527/1‑93 (MQ=255)
                               cTAAACTGTGAGTACCTTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGcc                                                     <  2:2637567/93‑1 (MQ=255)
                               cTAAACTGTGAGTACCCTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGcc                                                     <  2:2190578/93‑1 (MQ=255)
                                tAAACTGTGAGTACCCTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCa                                                    >  1:2644231/1‑93 (MQ=255)
                                tAAACTGTGAGTACCCTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCa                                                    >  2:1963872/1‑93 (MQ=255)
                                  aaCTGTGAGTACCCTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGc                                                  <  1:3216883/93‑1 (MQ=255)
                                   aCTGTGAGTACCCTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCt                                                 >  1:567409/1‑93 (MQ=255)
                                    cTGTGAGTACCCTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCtt                                                <  1:4398513/93‑1 (MQ=255)
                                     tgtgAGTACCCTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCTTc                                               <  2:2348858/93‑1 (MQ=255)
                                     tgtgAGTACCCTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCTTc                                               <  1:2501053/93‑1 (MQ=255)
                                     tgtgAGTACCCTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCTTc                                               <  1:1636377/93‑1 (MQ=255)
                                       tgAGTACCCTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCTTCGa                                             <  1:3197699/93‑1 (MQ=255)
                                       tgAGTACCCTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCTTCGa                                             >  2:3148505/1‑93 (MQ=255)
                                          gTACCCTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCTTCGACGt                                          >  2:2720584/1‑93 (MQ=255)
                                           tACCCTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCTTCGACGtt                                         <  2:607803/93‑1 (MQ=255)
                                           tACCCTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCTTCGACGtt                                         >  2:1589683/1‑93 (MQ=255)
                                            aCCCTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCTTCGACGTTc                                        >  2:1273477/1‑93 (MQ=255)
                                            aCCCTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCTTCGACGTTc                                        >  2:1792849/1‑93 (MQ=255)
                                            aCCCTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCTTCGACGTTc                                        <  1:2671580/93‑1 (MQ=255)
                                            aCCCTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTACGCGCCAGCTTCGACGTTc                                        >  2:1985460/1‑93 (MQ=255)
                                              ccTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCTTCGACGTTccc                                      >  1:2154317/1‑93 (MQ=255)
                                               cTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCTTCGACGTTcccc                                     >  1:664546/1‑93 (MQ=255)
                                                tttCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCTTCGACGTTCCCCa                                    >  1:3658257/1‑93 (MQ=255)
                                                tttCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCTTCGACGTTCCCCa                                    >  1:4002506/1‑93 (MQ=255)
                                                  tCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCTTCGACGTTCCCCAAc                                  >  2:3733027/1‑93 (MQ=255)
                                                    aCTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGAGCCAGCTTCGACGTTCCCCAACCg                                >  1:1399882/1‑93 (MQ=255)
                                                    aCTTTACCTGGCGCATTAGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCTTCGACGTTCCCCAACCg                                >  1:1973442/1‑93 (MQ=255)
                                                     cTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCTTCGACGTTCCCCAACCGc                               >  1:313650/1‑93 (MQ=255)
                                                     cTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCTTCGACGTTCCCCAACCGc                               >  2:3690750/1‑93 (MQ=255)
                                                      tttACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCTTCGACGTTCCCCAACCGCt                              <  2:2930809/93‑1 (MQ=255)
                                                       ttACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCTTCGACGTTCCCCAACCGCtt                             >  1:208633/1‑93 (MQ=255)
                                                         aCCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCTTCGACGTTCCCCAACCGCTTTc                           >  1:4440260/1‑93 (MQ=255)
                                                         aCCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCTTCGACGTTCCCCAACCGCTTTc                           <  1:1799254/93‑1 (MQ=255)
                                                           cTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCTTCGACGTTCCCCAACCGCTTTctc                         >  1:1033354/1‑92 (MQ=255)
                                                            tGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCTTCGACGTTCCCCAACCGCTTTctgc                        >  2:407071/1‑93 (MQ=255)
                                                            tGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCTTCGACGTTCCCCAACCGCTTTctgc                        <  2:2810851/93‑1 (MQ=255)
                                                            tGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCTTCGACGTTCCCCAACCGCTTTctgc                        <  2:2100/93‑1 (MQ=255)
                                                               cgcATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCTTCGACGTTCCCCAACCGCTTTCtgctgc                     >  1:1770787/1‑93 (MQ=255)
                                                                 cATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCTTCGACGTTCCCCAACCGCTTTCTGCTGCAg                   <  2:442566/93‑1 (MQ=255)
                                                                  aTTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTTTTGTTGTTGCGCGCCAGCTTCGACGTTCCCCAACCGCTTTCTGCTGCAGc                  <  1:798056/93‑1 (MQ=255)
                                                                  aTTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCTTCGACGTTCCCCAACCGCTTTCTGCTGCAGc                  >  1:880998/1‑93 (MQ=255)
                                                                   ttGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCTTCGACGTTCCCCAACCGCTTTCTGCTGCAGcc                 >  1:4123611/1‑93 (MQ=255)
                                                                   ttGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCTTCGACGTTCCCCAACCGCTTTCTGCTGCAGcc                 >  2:807998/1‑93 (MQ=255)
                                                                   ttGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCTTCGACGTTCCCCAACCGCTTTCTGCTGCAGcc                 >  2:1526726/1‑93 (MQ=255)
                                                                    tGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCTTCGACGTTCCCCAACCGCTTTCTGCTGCAGCCa                <  2:1391305/93‑1 (MQ=255)
                                                                      gTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCTTCGACGTTCCCCAACCGCTTTCTGCTGCAGCCAtc              <  2:3985333/93‑1 (MQ=255)
                                                                       tACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCTTCGACGTTCCCCAACCGCTTTCTGCTGCAGCCAtcg             >  2:4569552/1‑93 (MQ=255)
                                                                         cAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCTTCGACGTTCCCCAACCGCTTTCTGCTGCAGCCAtcgtc           >  1:3566967/1‑93 (MQ=255)
                                                                         cAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCTTCGACGTTCCCCAACCGCTTTCTGCTGCAGCCAtcgtc           <  2:738691/93‑1 (MQ=255)
                                                                          aaCGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCTTCGACGTTCCCCAACCGCTTTCTGCTGCAGCCAtcgtcg          >  2:3793735/1‑93 (MQ=255)
                                                                            cGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCTTCGACGTTCCCCAACCGCTTTCTGCTGCAGCCATCGTCGcc        <  2:1139871/93‑1 (MQ=255)
                                                                             gTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCTTCGACGTTCCCCAACCGCTTTCTGCTGCAGCCATCGTCGccc       >  2:3459902/1‑92 (MQ=255)
                                                                             gTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCTTCGACGTTCCCCAACCGCTTTCTGCTGCAGCCATCGTCGcca       >  1:3891576/1‑93 (MQ=255)
                                                                              tCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCTTCGACGTTCCCCAACCGCTTTCTGCTGCAGCCATCGTCGccac      <  2:2189072/93‑1 (MQ=255)
                                                                               ccTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCTTCGACGTTCCCCAACCGCTTTCTGCTGCAGCCATCGTCGccacc     >  2:1407890/1‑93 (MQ=255)
                                                                                cTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCTTCGACGTTCCCCAACCGCTTTCTGCTGCAGCCATCGTCGccacca    >  1:1552315/1‑93 (MQ=255)
                                                                                  ttCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCTTCGACGTTCCCCAACCGCTTTCTGCTGCAGCCATCGTCGCCACCATa  <  1:3438739/93‑1 (MQ=255)
                                                                                  ttCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCTTCGACGTTCCCCAACCGCTTTCTGCTGCAGCCATCGTCGCCACCATa  <  1:2804404/93‑1 (MQ=255)
                                                                                       ||                                                                                      
GACATAGGCGCTCACCGATTCTTTGACAGAACTAAACTGTGAGTACCCTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATCGATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCTTCGACGTTCCCCAACCGCTTTCTGCTGCAGCCATCGTCGCCACCATA  >  NC_000913/3734177‑3734351

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑