BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,003,385	Δ1 bp	coding (186/681 nt)	ycbF →	putative chaperone

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,003,384	0	A	.	94.3%	769.2 / 31.7	194	coding (185/681 nt)	ycbF	putative chaperone
Reads supporting (aligned to +/- strand): ref base A (2/0); major base . (122/59); minor base G (5/6); total (129/65)
Fisher's exact test for biased strand distribution p-value = 1.88e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.01e-01

Only 100 of 213 total aligned reads displayed.

GAATCGATATCTATTTTACTGACTAATACCAGTCAGGAATCCTGGCTTATTAACAGTAAAATCAACCGCCCAACGCGTTGGGCCGGGGGGGAAGCGTCGACAGTGCCAGCACCATTACTGGCCGCTCCGCCACTTATTCTCCTGAAGCCCGGTACGACAGGCACGTTGCGCTTGCTGAGA  >  NC_000913/1003293‑1003472

gaATCGATATCTATTTTACTGACTAATACCAGTCAGGAATCCTGGCTTATTAACAGTAAAATCAACCGCCCAACGCGTTGGGCCGGGGGGGag                                                                                         <  2:5162813/93‑2 (MQ=255)

gaATCGATATCTATTTTACTGACTAATACCAGTCAGGAATCCTGGCTTATTAACAGTAAAATCAACCGCCCAACGCGTTGGGCCGGGGGGGag                                                                                         >  2:1458431/1‑92 (MQ=255)

  aTCGATATCTATTTTACTGACTAATACCAGTCAGGAATCCTGGGTTATTAACAGTAAAATCAACCGCCCAACGCGTTGGGCCGGGGGGGagcg                                                                                       >  1:162525/1‑90 (MQ=255)

       tatCTATTTTACTGACTAATACCAGTCAGGAATCCTGGCTTATTAACAGTAAAATCAACCGCCCAACGCGTTGGGCCGGGGGGG‑AGCGTCGAc                                                                                 <  2:1004548/93‑1 (MQ=255)

            aTTTTACTGACTAATACCAGTCAGGAATCCTGGCTTATTAACAGTAAAATCAACCGCCCAACGCGTTGGGCCGGGGGGG‑AGCGTCGACAGTGc                                                                            >  1:881583/1‑93 (MQ=255)

             ttttACTGGCTAATACCAGTCAGGAATCCTGGCTTATTAACAGTAAAATCAACCGCCCACCGCGTTGGGCCGGGGGGG‑AGCGTCGACAGTGcc                                                                           <  1:967127/93‑1 (MQ=255)

               ttACTGACTAATACCAGTCAGGAATCCTGGCTTATTAACAGTAAAATCAACCGCCCAACGCGTTGGGCCGGGGGGG‑AGCGTCGACAGTGCCAg                                                                         >  1:835822/1‑93 (MQ=255)

                tACTGACTAATACCAGTCAGGAATCCTGGCTTATTAACAGTAAAATCAACCGCCCAACGCGTTGGGCCGGGGGGG‑AGCGTCGACAGTGCCAGc                                                                        >  2:2716247/1‑93 (MQ=255)

                   tGACTAATACCAGTCAGGAATCCTGGCTTATTAACAGTAAAATCAACCGCCCAACGCGTTGGGCCGGGGGGG‑AGCGTCGACAGTGCCAGCAcc                                                                     <  2:1550778/93‑1 (MQ=255)

                   tGACTAATACCAGTCAGGAATCCTGGCTTATTAACAGTAAAATCAACCGCCCAACGCGTTGGGCCGCGGGGG‑AGCGTCGACAGTGCCAGCAcc                                                                     >  1:3116703/1‑93 (MQ=255)

                    gACTAATACCAGTCAGGAATCCTGGCTTATTAACAGTAAAATCAACCGCCCAACGCGTTGGGCCGGGGGGG‑AGCGTCGACAGTGCCAGCACCa                                                                    >  2:2984671/1‑93 (MQ=255)

                     aCTAATACCAGTCAGGAATGCTGGCTTATTAACAGTAAAATCAACCGCCCAACGCGTTGGGCCGGGGGGG‑AGCGTCGACAGTGTCAGCAccgt                                                                   >  1:6564190/1‑91 (MQ=255)

                     aCTAATACCAGTCAGGAATCCTGGCTTATTAACAGTAAAATCAACCGCCCAACGCGTTGGGCCGGGGGGG‑AGCGTCGACAGTGCCAGCACCAt                                                                   >  1:231421/1‑93 (MQ=255)

                      cTAATACCAGTCAGGAATCCTGGCTTATTAACAGTAAAATCAACCGCCCAACGCGTTGGGCCGGGGGGG‑AGCGTCGACAGTGCCAGCACCAtt                                                                  >  1:4587025/1‑93 (MQ=255)

                        aaTACCAGTCAGGAATCCTGGCTTATTAACAGTAAAATCAACCGCCCAACGCGTTGGGCCGGGGGGG‑AGCGTCGACAGTGCCAGCACCATTAc                                                                <  1:4387813/93‑1 (MQ=255)

                        aaTACCAGTCAGGAATCCTGGCTTATTAACAGTAAAATCAACCGCCCAACGCGTTGGGCCGGGGGGG‑AGCGTCGACAGTGCCAGCACCATTAc                                                                >  2:3605374/1‑93 (MQ=255)

                         aTACGAGGCAGGAATCCTGTCTTATTAACAGGAAAATCAACCGCCCAACGGGTTGGGCCGGGGGGG‑AGCGTCGACAGTGCCAGCACCATTACt                                                               <  2:724472/93‑1 (MQ=255)

                         aTACCAGTCAGGAATCCTGGCTTATTAACAGTAAAATCAACCGCCCAACGCGTTGGGCCGGGGGGG‑AGCGTCGACAGTGCCAGCACCATTCCt                                                               >  2:4017307/1‑93 (MQ=255)

                         aTACCAGTCAGGAATCCTGGCTTATTAACAGTAAAATCAACCGCCCAACGCGTTGGGCCGGGGGGG‑AGCGTCGACAGTGCCAGCACCATTACt                                                               >  2:1171257/1‑93 (MQ=255)

                         aTACCAGTCAGGAATCCTGGCTTATTAACAGTAAAATCAACCGCCCAACGCGTTGGGCCGGGGGGG‑AGCGTCGACAGTGCCAGCACCATTACt                                                               >  1:5874588/1‑93 (MQ=255)

                          tACCAGTCAGGAATCCTGGCTTATTAACAGTAAAATCAACCGCCCAACGCGTTGGGCCGGGGGGG‑AGCGTCGACAGTGCCAGCACCATTACTg                                                              >  2:1318059/1‑93 (MQ=255)

                          aaCCAGTCAGGAATCCTGGCTTATTAACAGTAAAATCAACCGCCCAACGCGTTGGGCCGGGGGGG‑AGCGTCGACAGTGCCAGCACCATTACTg                                                              <  2:5206442/92‑1 (MQ=255)

                            ccAGTCAGGAATCCTGGCTTATTAACAGTAAAATCAACCGCCCAACGCGTTGGGCCGGGGGGG‑AGCGTCGACAGTGCCAGCACCATTACTGGc                                                            >  2:5406188/1‑93 (MQ=255)

                              aGTCAGGAATCCTGGCTTATTAACAGTAAAATCAACCGCCCAACGCGTTGGGCCGGGGGGG‑AGCGTCGACAGTGCCAGCACCATTACTGGCCg                                                          >  2:1748673/1‑93 (MQ=255)

                                 cAGGAATCCTGGCTTATTAACAGTAAAATCAACCGCCCAACGCGTTGGGCCGGGGGGG‑AGCGTCGACAGTGCCAGCACCATTACTGGCCGCTc                                                       >  1:3484891/1‑93 (MQ=255)

                                  aGGAATCCTGGCTTATTAACAGTAAAATCAACCGCCCAACGCGTTGGGCCGGGGGGG‑AGCGTCGACAGTGCCAGCACCATTACTGGCCGCTcc                                                      <  1:1041930/93‑1 (MQ=255)

                                  aGGAATCCTGGCTTATTAACAGTAAAATCAACCGCCCAACGCGTTGGGCCGGGGGGG‑AGCGTCGACAGTGCCAGCACCATTACTGGCCGCTcc                                                      <  1:5427779/93‑1 (MQ=255)

                                  aGGAATCCTGGCTTATTAACAGTAAAATCAACCGCCCAACGCGTGGGGCCGGGGGGG‑AGCGTCGACAGTGCCAGCACCATTACTGGCCGCTcc                                                      <  1:4218759/93‑1 (MQ=255)

                                   ggAATCCTGGCTTATTAACAGTAAAATCAACCGCCCAACGCGTTGGGCCGGGGGGG‑AGCGTCGACAGTGCCAGCACCATTACTGGCCGCTCCg                                                     >  2:281113/1‑93 (MQ=255)

                                   ggAATCCTGGCTTATTAACAGTAAAATCAACCGCCCAACGCGTTGGGCCGGGGGGG‑AGCGTCGACAGTGCCAGCACCATTACTGGCCGCTCCg                                                     >  1:3786426/1‑93 (MQ=255)

                                     aaTCCTGGCTTATTAACAGTAAAATCAACCGCCCAACGCGTTGGGCCGGGGGGG‑GGCGTCGACAGTGCCAGCACCATTACTGGCCGCTCCGcc                                                   >  1:4711496/1‑93 (MQ=255)

                                     aaTCCTGGCTTATTAACAGTAAAATCAACCGCCCAACGCGTTGGGCCGGGGGGG‑AGCGTCGACAGTGCCAGCACCATTACTGGCCGCTCCGcc                                                   >  2:4868599/1‑93 (MQ=255)

                                      aTCCTGGCTTATTAACAGTAAAATCAACCGCCCAACGCGTTGGGCCGGGGGGG‑AGCGTCGACAGTGCCAGCACCATTACTGGCCGCTCCGCCa                                                  >  1:737569/1‑93 (MQ=255)

                                      aTCCTGGCTTATTAACAGTAAAATCAACCGCCCAACGCGTTGGGCCGGGGGGG‑AGCGTCGACAGTGCCAGCACCATTACTGGCCGCTCCGCCa                                                  >  1:1595307/1‑93 (MQ=255)

                                       tCCTGGCTTATTAACAGTAAAATCAACCGCCCAACGCGTTGGGCCGGGGGGG‑AGCGTCGACAGTGCCAGCACCATTACTGGCCGCTCCGCCAc                                                 <  1:2177420/93‑1 (MQ=255)

                                       tCCTGGATTATTAACAGTAAAATCAACCGCCCACCGC‑TTGGGCCCGGGGGGGAGCGTCGACAGTGCCAGCACCATTACTGGCCGCTCCGCCAc                                                 <  1:1866642/93‑1 (MQ=255)

                                        ccTGGCTTATTAACAGTAAAATCAACCGCCCAACGCGTTGGGCCGGGGGGG‑AGCGTCGACAGTGCCAGCACCATTACTGGCCGCTCCGCCACt                                                <  1:3382823/93‑1 (MQ=255)

                                         cTGGTTTATCAACAGAAAAATCACCCGCCAAACGCGTGGGGCCGGGGGGG‑AGCGTCGACAGTGCCAGCACCATTACTGGCCGCTCCGCCACtt                                               <  2:1616561/93‑1 (MQ=255)

                                         cTGGCTTATTAACAGTAAAATCAACCGCCCAACGCGTTGGGCCGGGGGGG‑AGCGTCGACAGTGCCAGCACCATTACTGGCCGCTCCGCCACtt                                               <  1:3256282/93‑1 (MQ=255)

                                         cTGGCTTATTAACAGTAAAATCAACCGCCCAACGCGTTGGGCCGGGGGGG‑AGCGTCGACAGTGCCAGCACCATTACTGGCCGCTCCGCCACtt                                               >  1:6640552/1‑93 (MQ=255)

                                          tgttttattaAAAGAAAAACCACCCGCCCAACGCGTGGGGCCGGGGGGG‑AGCGTCGACAGTGCCAGCACCATTACTGGCCGCTCCGCCACTTa                                              <  1:5165959/89‑1 (MQ=255)

                                          tGGCTTATTAACAGTAAAATCAACCGCCCAACGCGTTGGGCCGGGGGGG‑AGCGTCGACAGTGCCAGCACCATTACTGGCCGCTCCGCCACTTa                                              >  2:730055/1‑93 (MQ=255)

                                          tGGCTTATTAACAGTAAAATCAACCGCCCAACGCGTTGGGCCGGGGGGG‑AGCGTCGACAGTGCCAGCACCATTACTGGCCGCTCCGCCACTTa                                              >  1:1778206/1‑93 (MQ=255)

                                           gggttattaACAGTAAAATCAACCGCCCAACGCGTTGGGCCGGGGGGG‑AGCGTCGACAGTGCCAGCACCATTACTGGCCGCTCCGCCACTTAt                                             >  1:5511125/4‑93 (MQ=255)

                                           ggCTTATTAACAGTAAAATCAACCGCCCAACGCGTTGGGCCGGGGGGG‑AGCGTCGACAGTGCCAGCACCATTACTGGCCGCTCCGCCACTTAt                                             >  2:814579/1‑93 (MQ=255)

                                           ggCTTATTAACAGTAAAATCAACCGCCCAACGCGTTGGGCCGGGGGGG‑AGCGTCGACAGTGCCAGCACCATTACTGGCCGCTCCGCCACTTAt                                             <  1:6694697/93‑1 (MQ=255)

                                           ggCTTATTAACAGTAAAATCAACCGCCCAACGCGTTGGGCCGGGGGGG‑AGCGTCGACAGTGCCAGCACCATTACTGGCCGCTCCGCCACTTAt                                             >  2:6595449/1‑93 (MQ=255)

                                            gCTTATTAACAGTAAAATCAACCGCCCAACGCGTTGGGCCGGGGGGG‑AGCGTCGACAGTGCCAGCACCATTACTGGCCGCTCCGCCACTTAtt                                            <  2:1837325/93‑1 (MQ=255)

                                             cTTATTAACAGTAAAATCAACCGCCCAACGCGTTGGGCCGGGGGGG‑AGCGTCGACAGTGCCAGCACCATTACTGGCCGCTCCGCCACTTATtc                                           >  2:6351200/1‑93 (MQ=255)

                                               tattaACAGTAAAATCAACCGCCCAACGCGTTGGGCCGGGGGGGAAGCGTCGACAGTGCCAGCACCATTACTGGCCGCTCCGCCACTTATtct                                          >  2:2293889/1‑93 (MQ=255)

                                               taataACAAAAAAATCACCCG‑CCAACCGGTTGGGCCCGGGGGGGAGCGTCGACAGTGCCAGCACCATTACTGGCCGCTCCGCCACTTATtctc                                         <  1:417273/90‑1 (MQ=255)

                                                attaACAGTAAAATCAACCGCCCAACGCGTTGGGCCGGGCGGG‑AGCGTCGACAGTGCCAGCACCATTACTGGCCGCTCCGCCACTTATTCTcc                                        >  1:1907431/1‑93 (MQ=255)

                                                 ttaCCAGGAAAACCAACCGCCCAACGCGTTGGGCCGGGGGGG‑AGCGTCGACAGTGCCAGCACCATTACTGGCCGCTCCGCCACTTATTCTCCt                                       <  1:2141700/93‑1 (MQ=255)

                                                 ttaACAGTAAAATCAACCGCCCAACGCGTTGGGCCGGGGGGG‑AGCGTCGACAGTGCCAGCACCATTACTGGCCGCTCCGCCACTTATTCTCCt                                       >  2:3294302/1‑93 (MQ=255)

                                                 ttaACAGTAAAATCAACCGCCCAACGCGTTGGGCCGGGGGGG‑AGCGTCGACAGTGCCAGCACCATTACTGGCCGCTCCGCCACTTATTCTCCt                                       >  2:5924672/1‑93 (MQ=255)

                                                  taACAGTAAAATCAACCGCCCAACGCGTTGGGCCGGGGGGG‑AGCGTCGACAGTGCCAGCACCATTACTGGCCGCTCCGCCACTTATTCTCCTg                                      >  2:1504107/1‑93 (MQ=255)

                                                   aaCAGTAAAATCAACCGCCCAACGCGTTGGGCCGGGGGGG‑AGCGTCGACAGTGCCAGCACCATTACTGGCCGCTCCGCCACTTATTCTCCTGa                                     >  1:1091642/1‑93 (MQ=255)

                                                   aaCAGTAAAATCAACCGCCCAACGCGTTGGGCCGGGGGGG‑AGCGTCGACAGTGCCAGCACCATTACTGGCCGCTCCGCCACTTATTCTCCTGa                                     >  2:4591456/1‑93 (MQ=255)

                                                   aaCAGTAAAATCAACCGCCCAACGCGTTGGGCCGGGGGGG‑AGCGTCGACAGTGCCAGCACCATTACTGGCCGCTCCGCCACTTATTCTCCTGa                                     <  2:6257044/93‑1 (MQ=255)

                                                   aaCAGTAAAATCAACCCCCCACCGCGTTGGGCCGGGGGGG‑AGCGTCGACAGTGCCAGCACCATTACTGGCCGCTCCGCCACTTATTCTCCTGa                                     <  2:5377254/93‑1 (MQ=255)

                                                    aCAGTAAAATCAACCGCCCAACGCGTTGGGCCGGGGGGG‑AGCGTCGACAGTGCCAGCACCATTACTGGCCGCTCCGCCACTTATTCTCCTGaa                                    >  2:6565775/1‑93 (MQ=255)

                                                     cAGTAAAATCAACCGCCCAACGCGTTGGGCCGGGGGGG‑AGCGTCGACAGTGCCAGCACCATTACTGGCCGCTCCGCCACTTATTCTCCTGAAg                                   >  2:1128500/1‑93 (MQ=255)

                                                     cAGTAAAATCAACCGCCCAACGCGTTGGGCCGGGGGGG‑AGCGTCGACAGTGCCAGCACCATTACTGGCCGCTCCGCCACTCATTCTCCTGAAg                                   >  1:5648384/1‑93 (MQ=255)

                                                      aGTAAAATCAACCGCCCAACGCGTTGGGCCGGGGGGG‑AGCGTCGACAGTGCCAGCACCATTACTGGCCGCTCCGCCACTTATTCTCCTGAAGc                                  >  1:6368141/1‑93 (MQ=255)

                                                       gaaaaaTCAACCGCCCAACGCGTGGGGCCGGGGGGG‑AGCGTCGACAGTGCCAGCACCATTACTGGCCGCTCCGCCACTTATTCTCCTGAAGcc                                 <  1:4330481/91‑1 (MQ=255)

                                                       gTAAAATCAACCGCCCAACGCGTTGGGCCGGGGGGG‑AGCGTCGACAGTGCCAGCACCATTACTGGCCGCTCCGCCACTTATTCTCCTGAAGcc                                 >  1:2513491/1‑93 (MQ=255)

                                                       aaaaaaTCACCCGCCCAACGCTTTGGGCCGGGGGGG‑AGCGTCGACAGTGCCAGCACCATTACTGGCCGCTCCGCCACTTATTCTACTGAAGcc                                 <  2:6133648/91‑1 (MQ=255)

                                                        tAAAATCACCCGCCCAACGCGTTGGGCCGGGGGGG‑AGCGTCGACAGTGCCAGCACCATTACTGGCCGCTCCGCCACTTATTCTCCTGAAGccc                                <  2:5070212/93‑1 (MQ=255)

                                                        tAAAATCAACCGCCCAACGCGTTGGGCCTGGGGGG‑AGCGTCGACAGTGCCAGCACCATTACTGGCCGCTCCGCCACTTATTCTCCTGAAGccc                                <  2:852565/93‑1 (MQ=255)

                                                        tAAAATCAACCGCCCAACGCGTTGGGCCGGGGGGG‑AGCGTCGACAGTGCCAGCACCATTACTGGCCGCTCCGCCACTTATTCTCCTGAAGccc                                <  1:4212736/93‑1 (MQ=255)

                                                          aaaTCAACCGCCCAACGCGTTGGGCCGGGGGGG‑AGCGTCGACAGTGCCAGCACCATTACTGGCCGCTCCGCCACTTATTCTCCTGAAGCCCgg                              >  1:3332003/1‑93 (MQ=255)

                                                             tCAACCGCCCACCGCGTTGGGCCGGGGGGG‑AGCGTCGACAGTGCCAGCACCATTACTGGCCGCTCCGCCACTTATTCTCCTGAAGCCCGGTAc                           >  2:6718213/1‑93 (MQ=255)

                                                             tCAACCGCCCAACGCGTTGGGCCGGGGGGG‑AGCGTCGACAGTGCCAGCACCATTACTGGCCGCTCCGCCACTTATTCTCCTGAAGCCCGGTAc                           >  1:2845897/1‑93 (MQ=255)

                                                               aaCCGCCCAACGCGTTGGGCCGGGGGGG‑AGCGTCGACAGTGCCAGCACCATTACTGGCCGCTCCGCCACTTATTCTCCTGAAGCCCGGTACgc                         >  2:3352517/1‑92 (MQ=255)

                                                                aCCGCCCAACGCGTTGGGCCGGGGGGG‑AGCGTCGACAGTGCCAGCACCATTACTGGCCGCTCCGCCACTTATTCTCCTGAAGCCCGGTACGAc                        <  1:2043264/93‑1 (MQ=255)

                                                                aCCGCCCAACGCGTTGGGCCGGGGGGG‑AGCGTCGACAGTGCCAGCACCATTACTGGCCGCTCCGCCACTTATTCTCCTGAAGCCCGGTACGAc                        >  2:3037174/1‑93 (MQ=255)

                                                                  cGCCCAACGCGTTGGGCCGGGGGGG‑AGCGTCGACAGTGCCAGCACCATTACTGGCCGCTCCGCCACTTATTCTCCTGAAGCCCGGTACGACAg                      >  2:2462721/1‑93 (MQ=255)

                                                                  cGCCCAACGCGTTGGGCCGGGGGGG‑AGCGTCGACAGTGCCAGCACCATTACTGGCCGCTCCGCCACTTATTCTCCTGAAGCCCGGTACGACAg                      <  2:773850/93‑1 (MQ=255)

                                                                  cGCCCAACGCGTTGGGCCGGGGGGG‑AGCGTCGACAGTGCCAGCACCATTACTGGCCGCTCCGCCACTTATTCTCCTGAAGCCCGGTACGACAg                      >  1:4157108/1‑93 (MQ=255)

                                                                  cGCCCAACGCGTGGGGCCGGGGGGG‑AGCGTCGACAGTGCCAGCACCATTACTGGCCGCTCCGCCACTTATTCTCCTGAAGCCCGGTACGACAg                      <  1:6297241/93‑1 (MQ=255)

                                                                   gCCCAACGCGTTGGGCCGGGGGGG‑AGCGTCGACAGTGCCAGCACCATTACTGGCCGCTCCGCCACTTATTCTCCTGAAGCCCGGTACGACAgg                     >  1:4640193/1‑93 (MQ=255)

                                                                     ccACCGCGTGGGGCCGGGGGGG‑AGCGTCGACAGTGCCAGCACCATTACTGGCCGCTCCGCCACTTATTCTCCTGAAGCCCGGTACGACAGGCa                   <  1:687887/93‑1 (MQ=255)

                                                                     ccAACGCGTTGGGCCGGGGGGG‑AGCGTCGACAGTGCCAGCACCATTACTGGCCGCTCCGCCACTTATTCTCCTGAAGCCCGGTACGACAGGCa                   >  2:2111748/1‑93 (MQ=255)

                                                                      cAACGCGTTGGGCCGGGGGGG‑AGCGTCGACAGTGCCAGCACCATTACTGGCCGCTCCGCCACTTATTCTCCTGAAGCCCGGTACGACAGGCAc                  >  2:6691689/1‑93 (MQ=255)

                                                                        aCGCGTTGGGCCGGGGGGG‑AGCGTCGACAGTGCCAGCACCATTACTGGCCGCTCCGCCACTTATTCTCCTGAAGCCCGGTACGACAGGCACGt                <  2:4284864/93‑1 (MQ=255)

                                                                        aCGCGTTGGGCCGGGGGGG‑AGCGTCGACAGTGCCAGCACCATTACTGGCCGCTCCGCCACTTATTCTCCTGAAGCCCGGTACGACAGGCACGt                <  2:199769/93‑1 (MQ=255)

                                                                        aCGCGTTGGGCCGGGGGGG‑AGCGTCGACAGTGCCAGCACCATTACTGGCCGCTCCGCCACTTATTCTCCTGAAGCCCGGTACGACAGGCACGt                <  1:1971759/93‑1 (MQ=255)

                                                                           gggcgggCCCGGGGGGGAGCGTCGACAGTGCCAGCACCATTACTGGCCGCTCCGCCACTTATTCTCCTGAAGCCCGGTACGACAGGCACGTTg              <  1:1169280/89‑1 (MQ=255)

                                                                           ctgggggCCGGGGGGG‑AGCGTCGACAGTGCCAGCACCATTACTGGCCGCTCCGCCACTTATTCTCCTGAAGCCCGGTACGACAGGCACGTTgc             <  1:1440355/89‑1 (MQ=255)

                                                                              tGGGCCGGGGGGG‑AGCGTCGACAGTGCCAGCACCATTACTGGCCGCTCCGCCACTTATTCTCCTGAAGCCCGGTACGACAGGCACGTTGCGCt          <  2:2797489/93‑1 (MQ=255)

                                                                              tGGGCCGGGGGGG‑AGCGTCGACAGTGCCAGCACCATTACTGGCCGCTCCGCCACTTATTCTCCTGAAGCCCGGTACGACAGGCACGTTGCGCt          <  1:1069533/93‑1 (MQ=255)

                                                                              ggggCCGGGGGGG‑AGCGTCGACAGTGCCAGCACCATTACTGGCCGCTCCGCCACTTATTCTCCTGAAGCCCGGTACGACAGGCACGTTGCGCt          <  1:3664409/92‑1 (MQ=255)

                                                                               gggCCGGGGGGG‑AGCGTCGACAGTGCCAGCACCATTACTGGCCGCTCCGCCACTTATTCTCCTGAAGCCCGGTACGACAGGCACGTTGCGCtt         <  1:1644506/93‑1 (MQ=255)

                                                                               gggCCGGGGGGG‑AGCGTCGACAGTGCCAGCACCATTACTGGCCGCTCCGCCACTTATTCTCCTGAAGCCCGGTACGACAGGCACGTTGCGCtt         <  2:5467603/93‑1 (MQ=255)

                                                                               gggCCAGGGGGG‑AGTGTCGACAGTGCCAGCACCATTACTGGCCGCTCCGCCACTTATTCTCCTGAAGCCCGGTACGACAGGCACGTTGCGCtt         <  1:5043748/93‑1 (MQ=255)

                                                                                ggCCGGGGGGG‑AGCGTCGACAGTGCCAGCACCATTACTGGCCGCTCCGCCACTTATTCTCCTGAAGCCCGGTACGACAGGCACGTTGCGCTTg        >  2:6437004/1‑93 (MQ=255)

                                                                                   ccggggggGAGCGTCGACAGTGCCAGCACCATTACTGGCCGCTCCGCCACTTATTCTCCTGAAGCCCGGTACGACAGGCACGTTGCGCTTGCt      >  2:6579322/3‑93 (MQ=255)

                                                                                     ggggggGAGCGTCGACAGTGCCAGCACCATTACTGGCCGCTCCGCCACTTATTCTCCTGAAGCCCGGTACGACAGGCACGTTGCGCTTGCTga    >  1:4006806/1‑93 (MQ=255)

                                                                                      ggggggAGCGTCGACAGTGCCAGCACCATTACTGGCCGCTCCGCCACTTATTCTCCTGAAGCCCGGTACGACAGGCACGTTGCGCTTGCTgag   >  1:5281566/1‑93 (MQ=255)

                                                                                       ggggGAGCGTCGACAGTGCCAGCACCATTACTGGCCGCTCCGCCACTTATTCTCCTGAAGCCCGGTACGACAGGCACGTTGCGCTTGCTgaga  >  1:271320/1‑93 (MQ=255)

GAATCGATATCTATTTTACTGACTAATACCAGTCAGGAATCCTGGCTTATTAACAGTAAAATCAACCGCCCAACGCGTTGGGCCGGGGGGGAAGCGTCGACAGTGCCAGCACCATTACTGGCCGCTCCGCCACTTATTCTCCTGAAGCCCGGTACGACAGGCACGTTGCGCTTGCTGAGA  >  NC_000913/1003293‑1003472

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 19 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`