BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,004,979	+G	coding (1511/1833 nt)	recQ →	ATP‑dependent DNA helicase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,004,979	1	.	G	100.0%	743.1 / NA	226	L504R (CTC→CGC)	recQ	ATP‑dependent DNA helicase
Reads supporting (aligned to +/- strand): ref base . (0/0); new base G (126/100); total (126/100)

Only 100 of 244 total aligned reads displayed.

GTGATCCGCCAGCTGATTCACCTCGGCCTGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCT‑CGCG‑‑GAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGAAATCGTTCGGCGGCAA  >  NC_000913/4004888‑4005060

gTGATCCGCCAGCTGATTCACCTCGGCCTGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCtg                                                                                     <  2:6619678/93‑2 (MQ=255)

gTGATCCGCCAGCTGATTCACCTCGGCCTGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCtg                                                                                     >  2:2714115/1‑92 (MQ=255)

gTGATCCGCCAGCTGATTCACCTCGGCCTGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCtg                                                                                     >  2:503752/1‑92 (MQ=255)

   aTCCGCCAGCTGATTCACCTCGGCCTGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCtgcgc                                                                                  >  2:6430746/1‑89 (MQ=255)

   aTCCGCCAGCTGATTCACCTCGGCCTGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCtgcgc                                                                                  <  1:1738299/93‑5 (MQ=255)

   aTCCGCCAGCTGATTCACCTCGGCCTGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCtgcgc                                                                                  <  2:4002487/93‑5 (MQ=255)

      cGCCAGCTGATTCACCTCGGCCTGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCg‑‑gc                                                                             <  1:3416481/93‑2 (MQ=255)

       gCCAGCTGATTCACCTCGGCCTGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCg‑‑gcg                                                                            >  2:6148291/1‑91 (MQ=255)

               aTTCACCTCGGCCTGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCGAATCctct                                                                      <  1:3426031/93‑1 (MQ=255)

               aTTCACCTCGGCCTGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCGAATCctct                                                                      >  2:5107831/1‑93 (MQ=255)

                ttCACCTCGGCCTGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCGAATCCTCtt                                                                     <  2:1263425/93‑1 (MQ=255)

                 tCACCTCGGCCTGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCGAATCCTCttt                                                                    <  1:5839311/93‑1 (MQ=255)

                 tCACCTCGGCCTGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCGAATCCTCttt                                                                    <  2:6209665/93‑1 (MQ=255)

                  cACCTCGGCCTGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCGAATCCTCTTTg                                                                   >  2:458711/1‑93 (MQ=255)

                  cACCTCGGCCTGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCGAATCCTCTTTg                                                                   <  1:5949510/93‑1 (MQ=255)

                  cACCTCGGCCTGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCGAATCCTCTTTg                                                                   >  2:5194942/1‑93 (MQ=255)

                   aCCTCGGCCTGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCGAATCCTCTTTGc                                                                  <  1:177896/93‑1 (MQ=255)

                     cTCGGCCTGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCGAATCCTCTTTGCaa                                                                >  2:1000895/1‑93 (MQ=255)

                        ggCCTGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCGAATCCTCTTTGCAACtt                                                             >  2:3648905/1‑93 (MQ=255)

                         gCCTGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCGAGTCCTCTTTGCAACTtg                                                            >  1:6422025/1‑93 (MQ=255)

                         gCCTGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCGAATCCTCTTTGCAACTtg                                                            >  2:962133/1‑93 (MQ=255)

                           cTGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCGAATCCTCTTTGCAACTtgcc                                                          >  2:3689593/1‑93 (MQ=255)

                           cTGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCGAATCCTCTTTGCAACTtgcc                                                          >  1:5756587/1‑93 (MQ=255)

                            tGGCGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCGAATCCTCTTTGCTACTtgccg                                                         >  1:931957/1‑93 (MQ=255)

                             ggTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGTTGCTGCGCGGCGAATCCTCTTTGCAACTtgccgt                                                        <  2:2647097/93‑1 (MQ=255)

                               tGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCGAATCCTCTTTGCAACTtgccgtgc                                                      >  1:4800151/1‑93 (MQ=255)

                                gACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCGAATCCTCTTTGCAACTtgccgtgcc                                                     >  1:757804/1‑93 (MQ=255)

                                 aCGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCGAATCCTCTTTGCAACTtgccgtgccg                                                    <  1:4331592/93‑1 (MQ=255)

                                  cGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCGAATCCTCTTTGCAACTTGCCGTGCcgc                                                   >  2:1076739/1‑93 (MQ=255)

                                      aaaTATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTAt                                               <  1:5455486/93‑1 (MQ=255)

                                      aaaTATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGACCGGTGCTGCGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTAt                                               >  2:435711/1‑93 (MQ=255)

                                        atatTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCg                                             >  1:4745426/1‑93 (MQ=255)

                                        atatTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCg                                             >  2:3865970/1‑93 (MQ=255)

                                          atTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTg                                           >  1:5033220/1‑93 (MQ=255)

                                          atTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTg                                           >  1:3187859/1‑93 (MQ=255)

                                           ttGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTgg                                          >  1:2462723/1‑93 (MQ=255)

                                            tGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTggg                                         >  1:2897219/1‑92 (MQ=255)

                                              cccAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGgcgc                                       <  1:3063807/93‑1 (MQ=255)

                                               ccAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCt                                      >  2:5530574/1‑93 (MQ=255)

                                               ccAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCt                                      >  2:4882245/1‑93 (MQ=255)

                                               ccAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCt                                      >  2:18352/1‑93 (MQ=255)

                                                cAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTc                                     >  1:2329023/1‑93 (MQ=255)

                                                cAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTc                                     >  1:2412192/1‑93 (MQ=255)

                                                cAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTc                                     >  1:1870463/1‑93 (MQ=255)

                                                  gCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTcca                                   >  2:5423355/1‑91 (MQ=255)

                                                  gCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCaa                                   >  1:2452273/1‑93 (MQ=255)

                                                  gCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCaa                                   >  1:556674/1‑93 (MQ=255)

                                                  gCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCaa                                   <  1:5405339/93‑1 (MQ=255)

                                                   cATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCaaa                                  <  1:222751/93‑1 (MQ=255)

                                                    aTTCTGCCCTACAAGTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAAc                                 <  1:4913147/93‑1 (MQ=255)

                                                    aTTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAAc                                 <  2:6118582/93‑1 (MQ=255)

                                                    aTTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAAc                                 >  1:5193470/1‑93 (MQ=255)

                                                    aTTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAAc                                 <  2:3951277/93‑1 (MQ=255)

                                                    aTTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAAc                                 <  2:4756940/93‑1 (MQ=255)

                                                     ttCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAAcc                                >  1:1457894/1‑93 (MQ=255)

                                                     ttCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAAcc                                >  2:3483112/1‑93 (MQ=255)

                                                       cTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGa                              >  2:1178402/1‑93 (MQ=255)

                                                        tGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGaa                             <  1:4429470/93‑1 (MQ=255)

                                                        tGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGaa                             <  2:2097567/93‑1 (MQ=255)

                                                         gCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGaaa                            <  2:4090014/93‑1 (MQ=255)

                                                         gCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGCATCGTGGCGCTCAAACCGcaa                            >  1:824080/1‑93 (MQ=255)

                                                         gCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCGAATCCTCTTTGCAACTTGACGTGCCGCGTATCGTGGCGCTCAAACCGaag                            >  1:6319605/1‑92 (MQ=255)

                                                          cccTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAg                           >  1:4140397/1‑93 (MQ=255)

                                                           ccTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGc                          >  1:1338950/1‑93 (MQ=255)

                                                           ccTACAACGGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGc                          <  1:2643967/93‑1 (MQ=255)

                                                            cTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCg                         <  2:714992/93‑1 (MQ=255)

                                                            cTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCg                         >  1:3604685/1‑93 (MQ=255)

                                                             tACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGa                        >  1:5517710/1‑93 (MQ=255)

                                                             tACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGa                        >  1:6037891/1‑93 (MQ=255)

                                                             tACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGa                        <  2:3390414/93‑1 (MQ=255)

                                                             tACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGa                        >  2:1440826/1‑93 (MQ=255)

                                                              acacCTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGAt                       <  1:4594845/93‑1 (MQ=255)

                                                              aCAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGAt                       <  2:6318475/93‑1 (MQ=255)

                                                              aCAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGAt                       >  1:66311/1‑93 (MQ=255)

                                                                aaCTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGc                     <  1:2755060/93‑1 (MQ=255)

                                                                aaCTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGc                     <  1:5650513/93‑1 (MQ=255)

                                                                 aCTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCa                    <  2:6719273/93‑1 (MQ=255)

                                                                 aCTGACAGAGCCCGCGCGCCCGGTGCTGCGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCa                    <  1:165913/93‑1 (MQ=255)

                                                                  cTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAg                   <  2:3345320/93‑1 (MQ=255)

                                                                  cTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAg                   <  2:5907540/93‑1 (MQ=255)

                                                                   tGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGa                  >  2:3150805/1‑93 (MQ=255)

                                                                   tGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGa                  >  1:5208787/1‑93 (MQ=255)

                                                                    gACAGAGGCCGCGCGCCCGGTGCTGCGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGaa                 >  2:5719711/1‑93 (MQ=255)

                                                                      cAGAGGCCGCGCGCCCGGTGCTGCGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGAAAt               <  2:4105532/93‑1 (MQ=255)

                                                                      cAGAGGCCGCGCGCCCGGTGCTGCGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGAAAt               >  2:2256815/1‑93 (MQ=255)

                                                                      cAGAGGCCGCGCGCCCGGTGCTGCGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGAAAt               <  2:5369164/93‑1 (MQ=255)

                                                                      cAGAGGCCGCGCGCCCGGTGCTGCGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGAAAt               >  1:3863586/1‑93 (MQ=255)

                                                                       agagGCCGCGCGCCCGGTGCTGCGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGAAATc              >  2:2581257/1‑93 (MQ=255)

                                                                        gagGCCGCGCGCCCGGTGCTGCGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGAAATCg             >  1:5998168/1‑93 (MQ=255)

                                                                        gagGCCGCGCGCCCGGTGCTGCGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGAAATCg             <  2:1346223/93‑1 (MQ=255)

                                                                         agGCCGCGCGCCCGGTGCTGCGCGGCGAATCCTCTTTGCGACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGAAATCGt            <  1:3953792/93‑1 (MQ=255)

                                                                         agGCCGCGCGCCCGGTGCTGCGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGAAATCGt            >  1:3649860/1‑93 (MQ=255)

                                                                          ggCCGCGCGCCCGGTGCTGCGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGAAATCGtt           <  2:637795/93‑1 (MQ=255)

                                                                          ggCCGCGCGCCCGGTGCTGCGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGAAATCGtt           <  1:6146432/93‑1 (MQ=255)

                                                                          ggCCGCGCGCCCGGTGCTGCGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGAAATCGtt           >  2:2528771/1‑93 (MQ=255)

                                                                            ccGCGCGCCCGGTGCTGCGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGAAATCGTTcg         <  1:1013991/93‑1 (MQ=255)

                                                                             cgcgcgCCCGGTGCTGCGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGAAATCGTTcgg        >  1:4095816/1‑93 (MQ=255)

                                                                               cgcgcCCGGTGCTGCGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGAAATCGTTcggcg      <  2:2330605/93‑1 (MQ=255)

                                                                                 cgcCCGGTGCTGCGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGAAATCGTTCggcggc    >  2:1795183/1‑93 (MQ=255)

                                                                                  gcCCGGTGCTGCGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGAAATCGTTCGGCGGCa   <  2:3457588/93‑1 (MQ=255)

GTGATCCGCCAGCTGATTCACCTCGGCCTGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCT‑CGCG‑‑GAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGAAATCGTTCGGCGGCAA  >  NC_000913/4004888‑4005060

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 20 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`