Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,095,766 (T)7→8 coding (701/987 nt) ycdU → hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,095,7591.T97.9% 723.0 / 5.0 194coding (694/987 nt)ycdUhypothetical protein
Reads supporting (aligned to +/- strand):  ref base . (1/3);  new base T (73/117);  total (74/120)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01

Only 100 of 235 total aligned reads displayed.

CGATAATTGCATTGCTCATTCTTTGTGGCGTAGTGTTGGCGATTAATCTGTTTTTTCTGCGCTTCTCCAAGCGGTACGCTTTTCTGGGCAGG‑C‑TTTTTTTGCAGGAAATCGATCTGTTTTTCTCCAGTGAAAATGCGTTGTCGACCTTTTTTGCCAAGCCGCTTTGGCTTCGGTTATTCATATT  >  NC_000913/1095667‑1095850
                                                                                              |                                                                                           
cGATAATTGCATTGCTCATTCTTTGTGGCGTAGTGTTGGCGATTAATCTGTTTTTTCTGCGCTTCTCCAAGCGGTACGCTTTTCTGGGCAGG‑c                                                                                              >  2:1962878/1‑93 (MQ=255)
  aTAATTGCATTGCTCATTCTTTGTGGCGTAGTGTTGGCGATTAATCTGTTTTTTCTGCGCTTCTCCAAGCGGTACGCTTTTCTGGGCAGG‑C‑tt                                                                                           <  2:1477407/93‑1 (MQ=255)
  aTAATTGCATTGCTCATTCTTTGTGGCGTAGTGTTGGCGATTAATCTGTTTTTTCTGCGCTTCTCCAAGCGGTACGCTTTTCTGGGCAGG‑C‑tt                                                                                           >  1:6029175/1‑93 (MQ=255)
  aTAATTGCATTGCTCATTCTTTGTGGCGTAGTGTTGGCGATTAATCTGTTTTTTCTGCGCTTCTCCAAGCGGTACGCTTTTCTGGGCAGG‑C‑tt                                                                                           <  2:726925/93‑1 (MQ=255)
   tAATTGCATTGCTCATTCTTTGTGGCGTAGTGTTGGCGATTAATCTGTTTTTTCTGCGCTTCTCCAAGCGGTACGCTTTTCTGGGCAGG‑C‑ttt                                                                                          <  2:394287/93‑1 (MQ=255)
     attgcattgcTCATTCTTTGTGGCGTAGTGTTGGCGATTAATCTGTTTTTTCTGCGCTTCTCCAAGCGGTACGCTTTTCTGGGCAGG‑C‑ttttt                                                                                        <  2:504407/93‑1 (MQ=255)
     attgcattgcTCATTCTTTGTGGCGTAGTGTTGGCGATTAATCTGTTTTTTCTGCGCTTCTCCAAGCGGTACGCTTTTCTGGGCAGG‑C‑ttttt                                                                                        >  2:2085148/1‑93 (MQ=255)
     attgcattgcTCATTCTTTGTGGCGTAGTGTTGGCGATTAATCTGTTTTTTCTGCGCTTCTCCAAGCGGTACGCTTTTCTGGGCAGG‑C‑ttttt                                                                                        <  1:2699428/93‑1 (MQ=255)
     attgcattgcTCATTCTTTGTGGCGTAGTGTTGGCGATTAATCTGTTTTTTCTGCGCTTCTCCAAGCGGTACGCTTTTCTGGGCAGG‑C‑ttttt                                                                                        <  1:3002244/93‑1 (MQ=255)
        gcattgcTCATTCTTTGTGGCGTAGTGTTGGCGATTAATCTGTTTTTTCTGCGCTTCTCCAAGCGGTACGCTTTTCTGGGCAGG‑C‑tttttttt                                                                                     <  2:5250321/93‑2 (MQ=255)
        gcattgcTCATTCTTTGTGGCGTAGTGTTGGCGATTAATCTGTTTTTTCTGCGCTTCTCCAAGCGGTACGCTTTTCTGGGCAGG‑C‑tttttttt                                                                                     >  2:4501151/1‑92 (MQ=255)
          attgcTCATTCTTTGTGGCGTAGTGTTGGCGATTAATCTGTTTTTTCTGCGCTTCTCCAAGCGGTACGCTTTTCTGGGCATG‑C‑ttttttttgc                                                                                   >  1:1786035/1‑90 (MQ=255)
          attgcTCATTCTTTGTGGCGTAGTGTTGGCGATTAATCTGTTTTTTCTGCGCTTCTCCAAGCGGTACGCTTTTCTGGGCAGG‑C‑ttttttttgc                                                                                   >  1:505893/1‑90 (MQ=255)
          attgcTCATTCTTTGTGGCGTAGTGTTGGCGATTAATCTGTTTTTTCTGCGCTTCTCCAAGCGGTACGCTTTTCTGGGCAGG‑C‑ttttttttgc                                                                                   <  2:2042687/93‑4 (MQ=255)
          attgcTCATTCTTTGTGGCGTAGTGTTGGCGATTAATCTGTTTTTTCTGCGCTTCTCCAAGCGGTACGCTTTTCTGGGCAGG‑C‑ttttttttgc                                                                                   >  2:2314175/1‑90 (MQ=255)
          attgcTCATTCTTTGTGGCGTAGTGTTGGCGATTAATCTGTTTTTTCTGCGCTTCTCCAAGCGGTACGCTTTTCTGGGCAGG‑C‑ttttttttgc                                                                                   >  2:4352711/1‑90 (MQ=255)
          attgcTCATTCTTTGTGGCGTAGTGTTGGCGATTAATCTGTTTTTTCTGCGCTTCTCCAAGCGGTACGCTTTTCTGGGCAGG‑C‑ttttttttgc                                                                                   >  1:3445772/1‑90 (MQ=255)
           ttgcTCATTCTTTGTGGCGTAGTGTTGGCGATTAATCTGTTTTTTCTGCGCTTCTCCAAGCGGTACGCTTTTCTGGGCAGG‑C‑ttttttttgca                                                                                  >  1:881060/1‑89 (MQ=255)
           ttgcTCATTCTTTGTGGCGTAGTGTTGGCGATTAATCTGTTTTTTCTGCGCTTCTCCAAGCGGTACGCTTTTCTGGGCAGG‑C‑ttttttttgca                                                                                  <  1:6645242/93‑5 (MQ=255)
             gcTCATTCTTTGTGGCGTAGTGTTGGCGATTAATCTGTTTTTTCTGCGCTTCTCCAAGCGGTACGCTTTTCTGGGCAGG‑CTTTTTTTTGCAgg                                                                                 <  1:1638276/93‑1 (MQ=255)
                cATTCTTTGTGGCGTAGTGTTGGCGATTAATCTGTTTTTTCTGCGCTTCTCCAAGCGGTACGCTTTTCTGGGCAGG‑CTTTTTTTTGCAGGaaa                                                                              >  1:1206360/1‑93 (MQ=255)
                 aTTCTTTGTGGCGTAGTGTTGGCGATTAATCTGTTTTTTCTGCGCTTCTCCAAGCGGTACGCTTTTCTGGGCAGG‑CTTTTTTTTGCAGGAAAt                                                                             >  2:4571484/1‑93 (MQ=255)
                 aTTCTTTGTGGCGTAGTGTTGGCGATTAATCTGTTTTTTCTGCGCTTCTCCAAGCGGTACGCTTTTCTGGGCAGG‑CTTTTTTTTGCAGGAAAt                                                                             >  1:5200142/1‑93 (MQ=255)
                  ttCTTTGTGGCGTAGTGTTGGCGATTAATCTGTTTTTTCTGCGCTTCTCCAAGCGGTACGCTTTTCTGGGCAGG‑CTTTTTTTTGCAGGAAATc                                                                            >  1:4310107/1‑93 (MQ=255)
                   tCTTTGTGGCGTAGTGTTGGCGATTAATCTGTTTTTTCTGCGCTTCTCCAAGCGGTACGCTTTTCTGGGCAGG‑CTTTTTTTTGCAGGAAATCg                                                                           <  1:2248474/93‑1 (MQ=255)
                   tCTTTGTGGCGTAGTGTTGGCGATTAATCTGTTTTTTCTGCGCTTCTCCAAGCGGTACGCTTTTCTGGGCAGG‑CTTTTTTTTGCAGGAAATCg                                                                           <  2:1151679/93‑1 (MQ=255)
                   tCTTTGCGGCGTAGTGTTGGCGATTAATCTGTTTTTTCTGCGCTTCTCCAAGCGGTACGCTTTTCTGGGCAGG‑CTTTTTTTTGCAGGAAATCg                                                                           >  2:6114491/1‑93 (MQ=255)
                    cTTTGTGGCGTAGTGTTGGCGATTAATCTGTTTTTTCTGCGCTTCTCCAAGCGGTACGCTTTTCTGGGCAGG‑CTTTTTTTTGCAGGAAATCGa                                                                          <  1:1832930/93‑1 (MQ=255)
                     tttGTGGCGTAGTGTTGGCGATTAATCTGTTTTTTCTGCGCTTCTCCAAGCGGTACGCTTTTCTGGGCAGG‑CTTTTTTTTGCAGGAAATCGAt                                                                         >  2:4666750/1‑93 (MQ=255)
                     tttGTGGCGTAGTGTTGGCGATTAATCTGTTTTTTCTGCGCTTCTCCAAGCGGTACGCTTTTCTGGGCAGG‑CTTTTTTTTGCAGGAAATCGAt                                                                         <  1:6355081/93‑1 (MQ=255)
                     tttGTGGCGTAGTGTTGGCGATTAATCTGTGTTTTCTGCGCTTCTCCAAGCGGTACGCTTTTCTGGGCAGG‑CTTTTTTTTGCAGGAAATCGAt                                                                         <  1:5150284/93‑1 (MQ=255)
                      ttGTGGCGTAGTGTTGGCGATTAATCTGTTTTTTCTGCGCTTCTCCAAGCGGTACGCTTTTCTGGGCAGG‑CTTTTTTTTGCAGGAAATCGATc                                                                        <  2:3027266/93‑1 (MQ=255)
                      ttGTGGCGTAGTGTTGGCGATTAATCTGTTTTTTCTGCGCTTCTCCAAGCGGTACGCTTTTCTGGGCAGG‑CTTTTTTTTGCAGGAAATCGATc                                                                        <  2:1686748/93‑1 (MQ=255)
                      ttGTGGCGTAGTGTTGGCGATTAATCTGTTTTTTCTGCGCTTCTCCAAGCGGTACGCTTTTCTGGGCAGG‑CTTTTTTTTGCAGGAAATCGATc                                                                        <  1:3937241/93‑1 (MQ=255)
                       tgtgGCGTAGTGTTGGCGATTAATCTGTTTTTTCTGCGCTTCTCCAAGCGGTACGCTTTTCTGGGCAGG‑CTTTTTTTTGCAGGAAATCGATCt                                                                       <  2:941010/93‑1 (MQ=255)
                          ggCGTAGTGTTGGCGATTAATCTGTTTTTTCTGCGCTTCTCCAAGCGGTACGCTTTTCTGGGCAGG‑CTTTTTTTTGCAGGAAATCGATCTGtt                                                                    <  2:1350014/93‑1 (MQ=255)
                           gCGTAGTGTTGGCGATTAATCTGTTTTTTCTGCGCTTCTCCAAGCGGTACGCTTTTCTGGGCAGG‑CTTTTTTTTGCAGGAAATCGATCTGttt                                                                   <  1:6699660/93‑1 (MQ=255)
                           gCGTAGTGTTGGCGATTAATCTGTTTTTTCTGCGCTTCTCCAAGCGGTACGCTTTTCTGGGCAGG‑CTTTTTTTTGCAGGAAATCGATCTGttt                                                                   <  2:662176/93‑1 (MQ=255)
                           gCGTAGTGTTGGCGATTAATCTGTTTTTTCTGCGCTTCTCCAAGCGGTACGCTTTTCTGGGCAGG‑CTTTTTTTTGCAGGAAATCGATCTGttt                                                                   <  1:5985120/93‑1 (MQ=255)
                            cGTAGTGTTGGCGATTAATCTGTTTTTTCTGCGCTTCTCCAAGCGGTACGCTTTTCTGGGCAGG‑CTTTTTTTTGCAGGAAATCGATCTGtttt                                                                  <  2:5555186/93‑1 (MQ=255)
                              tAGTGTTGGCGATTAATCTGTTTTTTCTGCGCTTCTCCAAGCGGTACGCTTTTCTGGGCAGG‑CTTTTTTTTGCAGGAAATCGATCTGTTTTtc                                                                <  2:361200/93‑1 (MQ=255)
                                 tgtTGGCGATTAATCTGTTTTTTCTGCGCTTCTCCAAGCGGTACGCTTTTCTGGGCAGG‑CTTTTTTTTGCAGGAAATCGATCTGTTTTTCTcc                                                             <  1:1235891/93‑1 (MQ=255)
                                 tgtTGGCGATTAATCTGTTTTTTCTGCGCTTCTCCAAGCGGTACGCTTTTCTGGGCAGG‑CTTTTTTTTGCAGGAAATCGATCTGTTTTTCTcc                                                             <  1:1502242/93‑1 (MQ=255)
                                 tgtTGGCGATTAATCTGTTTTTTCTGCGCTTCTCCAAGCGGTACGCTTTTCTGGGCAGG‑CTTTTTTTTGCAGGAAATCGATCTGTTTTTCTcc                                                             <  1:6288136/93‑1 (MQ=255)
                                  gtTGGCGATTAATCTGTTTTTTCTGCGCTTCTCCAAGCGGTACGCTTTTCTGGGCAGG‑CTTTTTTTTGCAGGAAATCGATCTGTTTTTCTCCa                                                            <  1:4671108/93‑1 (MQ=255)
                                   ttGGCGATTAATCTGTTTTTTCTGCGCTTCTCCAAGCGGTACGCTTTTCTGGGCAGG‑CTTTTTTTTGCAGGAAATCGATCTGTTTTTCTCCAg                                                           >  1:3413402/1‑93 (MQ=255)
                                    tGGCGATTAATCTGTTTTTTTTGCGCTTCTCCAAGCGGTACGCTTTTCTGGGCAGG‑CTTTTTTTTGCAGGAAATCGATCTGTTTTTCTCCAGt                                                          <  1:5012488/93‑1 (MQ=255)
                                    tGGCGATTAATCTGTTTTTTCTGCGCTTCTCCAAGCGGTACGCTTTTCTGGGCAGG‑CTTTTTTTTGCAGGAAATCGGTCTGTTTTTCTCCAGt                                                          >  1:6222526/1‑93 (MQ=255)
                                       cGATTAATCTGTTTTTTCTGCGCTTCTCCAAGCGGTACGCTTTTCTGGGCAGG‑CTTTTTTTTGCAGGAAATCGATCTGTTTTTCTCCAGTGaa                                                       <  1:5836174/93‑1 (MQ=255)
                                       cGATTAATCTGTTTTTTCTGCGCTTCTCCAAGCGGTACGCTTTTCTGGGCAGG‑CTTTTTTTTGCAGGAAATCGATCTGTTTTTCTCCAGTGaa                                                       <  2:5768776/93‑1 (MQ=255)
                                         aTTAATCAGTTTTTTCTGCGCTTCTCCAAGCGGTACGCTTTTCTGGGCAGG‑CTTTTTTTTGCAGGAAATCGATCTGTTTTTCTCCAGTGaaaa                                                     >  1:5400462/1‑93 (MQ=255)
                                          ttAATCTGTTTTTTCTGCGCTTCTCCAAGCGGTACGCTTTTCTGGGCAGG‑CTTTTTTTTGCAGGAAATCGATCTGTTTTTCTCCAGTGAAAAt                                                    <  1:5599534/93‑1 (MQ=255)
                                          ttAATCTGTTTTTTCTGCGCTTCTCCAAGCGGTACGCTTTTCTGGGCAGG‑CTTTTTTTTGCAGGAAATCGATCTGTTTTTCTCCAGTGAAAAt                                                    >  1:4795032/1‑93 (MQ=255)
                                          ttAATCTGTTTTTTCTGCGCTTCTCCAAGCGGTACGCTTTTCTGGGCAGG‑CTTTTTTTTGCAGGAAATCGATCTGTTTTTCTCCAGTGAAAAt                                                    <  1:5442471/93‑1 (MQ=255)
                                           tAATCTGTTTTTTCTGCGCTTCTCCAAGCGGTACGCTTTTCTGGGCAGG‑CTTTTTTTTGCAGGAAATCGATCTGTTTTTCTCCAGTGAAAATg                                                   >  1:4619268/1‑93 (MQ=255)
                                            aaTCTGTTTTTTCTGCGCTTCTCCAAGCGGTACGCTTTTCTGGGCAGG‑CTTTTTTTTGCAGGAAATCGATCTGTTTTTCTCCAGTGAAAATGc                                                  >  1:288525/1‑93 (MQ=255)
                                            aaTCTGTTTTTTCTGCGCTTCTCCAAGCGGTACGCTTTTCTGGGCAGG‑CTTTTTTTTGCAGGAAATCGATCTGTTTTTCTCCAGTGAAAATGc                                                  <  1:5722340/93‑1 (MQ=255)
                                            aaTCTGTTTTTTCTGCGCTTCTCCAAGCGGTACGCTTTTCTGGGCAGG‑CTTTTTTTTGCAGGAAATCGATCTGTTTTTCTCCAGTGAAAATGc                                                  >  1:4466751/1‑93 (MQ=255)
                                            aaTCTGTTTTTTCTGCGCTTCTCCAAGCGGTACGCTTTTCTGGGCAGG‑CTTTTTTTTGCAGGAAATCGATCTGTTTTTCTCCAGTGAAAATGc                                                  <  2:5755803/93‑1 (MQ=255)
                                             aTCTGTTTTTTCTGCGCTTCTCCAAGCGGTACGCTTTTCTGGGCAGG‑CTTTTTTTTGCAGGAAATCGATCTGTTTTTCTCCAGTGAAAATGCg                                                 >  1:3655690/1‑93 (MQ=255)
                                               cTGTTTTTTCTGCGCTTCTCCAAGCGGTACGCTTTTCTGGGCAGG‑CTTTTTTTTGCAGGAAATCGATCTGTTTTTCTCCAGTGAAAATGCGtt                                               <  1:3876093/93‑1 (MQ=255)
                                                 gTTTTTTCTGCGCTTCTCCAAGCGGTACGCTTTTCTGGGCAGG‑CTTTTTTTTGCAGGAAATCGATCTGTTTTTCTCCAGTGAAAATGCGTTGt                                             <  2:6357737/93‑1 (MQ=255)
                                                 gTTTTTTCTGCGCTTCTCCAAGCGGTACGCTTTTCTGGGCAGG‑CTTTTTTTTGCAGGAAATCGATCTGTTTTTCTCCAGTGAAAATGCGTTGt                                             <  2:277141/93‑1 (MQ=255)
                                                         tGCGCTTCTCCAAGCGGTACGCTTTTCTGGGCAGG‑CTTTTTTTTGCAGGAAATCGATCTGTTTTTCTCCAGTGAAAATGCGTTGTCGACCttt                                     >  1:3627170/1‑93 (MQ=255)
                                                          gcgcTTCTCCAAGCGGTACGCTTTTCTGGGCAGT‑CTTTTTTTTGCAGGAAATCCATCTGTCTTTCTCCAGTGAAAATGCGTTGTCGACCtttt                                    <  1:3953438/93‑1 (MQ=255)
                                                          gcgcTTCTCCAAGCGGTACGCTTTTCTGGGCAGG‑CTTTTTTTTGCAGGAAATCGATCTGTTTTTCTCCAGTGAAAATGCGTTGTCGACCtttt                                    <  1:1974402/93‑1 (MQ=255)
                                                           cgcTTCTCCAAGCGGTACGCTTTTCTGGGCAGG‑CTTTTTTTTGCAGGAAATCGATCTGTTTTTCTCCAGTGAAAATGCGTTGTCGACCttttt                                   <  2:3098476/93‑1 (MQ=255)
                                                           cgcTTCTCCAAGCGGTACGCTTTTCTGGGCAGG‑CTTTTTTTTGCAGGAAATCGATCTGTTTTTCTCCAGTGAAAATGCGTTGTCGACCttttt                                   <  2:2880891/93‑1 (MQ=255)
                                                             cTTCTCCAAGCGGTACGCTTTTCTGGGCAGG‑CTTTTTTTTGCAGGAAATCGATCTGTTTTTCTCCAGTGAAAATGCGTTGTCGACCTTTTTTg                                 >  1:4081417/1‑93 (MQ=255)
                                                             cTTCTCCAAGCGGTACGCTTTTCTGGGCAGG‑CTTTTTTTTGCAGGAAATCGATCTGTTTTTCTCCAGTGAAAATGCGTTGTCGACCTTTTTTg                                 <  1:3533304/93‑1 (MQ=255)
                                                             cTTCTCCAAGCGGTACGCTTTTCTGGGCAGG‑CTTTTTTTTGCAGGAAATCGATCTGTTTTTCTCCAGTGAAAATGCGTTGTCGACCTTTTTTg                                 <  1:3318081/93‑1 (MQ=255)
                                                              ttCTCCAAGCGGTACGCTTTTCTGGGCAGG‑CTTTTTTTTGCAGGAAATCGCTCTGTTTTTCTCCAGTGAAAATGCGTTGTCGACCTTTTTTGc                                >  1:6383537/1‑93 (MQ=255)
                                                               tctcCAAGCGGTACGCTTTTCTGGGCAGG‑CTTTTTTTTGCAGGAAATCGATCTGTTTTTCTCCAGTGAAAATGCGTTGTCGACCTTTTTTGcc                               <  2:6580225/93‑1 (MQ=255)
                                                               tctcCAAGCGGTACGCTTTTCTGGGCAGG‑CTTTTTTTTGCAGGAAATCGATCTGTTTTTCTCCAGTGAAAATGCGTTGTCGACCTTTTTTGcc                               >  2:2278886/1‑93 (MQ=255)
                                                                 tcCAAGCGGTACGCTTTTCTGGGCAGGAT‑TTTTTTTGCAGGAAATCGATCTGTTTTTCTCCAGTGAAAATGCGTTGTCGACCTTTTTTGCCaa                             >  1:96456/1‑93 (MQ=255)
                                                                 tcCAAGCGGTACGCTTGTCTGGGCAGG‑CTTTTTTTTGCAGGAAATCGATCTGTTTTTCTCCAGTGAAAATGCGTTGTCGACCTTTTTTGCCaa                             <  2:3245251/93‑1 (MQ=255)
                                                                  ccAAGCGGTACGCTTTTCTGGGCAGG‑CTTTTTTTTGCAGGAAATCGATCTGTTTTTCTCCAGTGAAAATGCGTTGTCGACCTTTTTTGCCAAg                            >  1:310220/1‑93 (MQ=255)
                                                                   cAAGCGGTACGCTTTTCTGGGCAGG‑CTTTTTTTTGCAGGAAATCGATCTGTTTTTCTCCAGTGAAAATGCGTTGTCGACCTTTTTTGCCAAGc                           <  2:4984098/93‑1 (MQ=255)
                                                                    aaGCGGTACGCTTTTCTGGGCAGG‑CTTTTTTTTGCAGGAAATCGATCTGTTTTTCTCCAGTGAAAATGCGTTGTCGACCTTTTTTGCCAAGcc                          >  1:2611277/1‑93 (MQ=255)
                                                                    aaGCGGTACGCTTTTCTGGGCAGG‑CTTTTTTTTGCAGGAAATCGATCTGTTTTTCTCCAGTGAAAATGCGTTGTCGACCTTTTTTGCCAAGcc                          <  2:535362/93‑1 (MQ=255)
                                                                     aGCGGTACGCTTTTCTGGGCAGG‑CTTTTTTTTGCAGGAAATCGATCTGTTTTTCTCCAGTGAAAATGCGTTGTCGACCTTTTTTGCCAAGCCg                         >  2:352755/1‑93 (MQ=255)
                                                                       cGGTACGCTTTTCTGGGCAGG‑CTTTTTTTTGCAGGAAATCGATCTGTTTTTCTCCAGTGAAAATGCGTTGTCGACCTTTTTTGCCAAGCCGCt                       <  1:1034682/93‑1 (MQ=255)
                                                                         gTACGCTTTTCTGGGCAGG‑CTTTTTTTTGCAGGAAATCGATCTGTTTTTCTCCAGTGAAAATGCGTTGTCGACCTTTTTTGCCAAGCCGCttt                     <  1:4166992/93‑1 (MQ=255)
                                                                         gTACGCTTTTCTGGGCAGG‑CTTTTTTTTGCAGGAAATCGATCTGTTTTTCTCCAGTGAAAATGCGTTGTCGACCTTTTTTGCCAAGCCGCttt                     <  1:2184987/93‑1 (MQ=255)
                                                                         gTACGCTTTTCTGGGCAGG‑CTTTTTTTTGCAGGAAATCGATCTGTTTTTCTCCAGTGAAAATGCGTTGTCGACCTTTTTTGCCAAGCCGCttt                     >  1:3344221/1‑93 (MQ=255)
                                                                          tACGCTTTTCTGGGCAGG‑CTTTTTTTTGCAGGAAATCGATCTGTTTTTCTCCAGTGAAAATGCGTTGTCGACCTTTTTTGCCAAGCCGCTTTg                    >  1:3618937/1‑93 (MQ=255)
                                                                           aCGCTTTTCTGGGCAGG‑CTTTTTTTTGCAGGAAATCGATCTGTTTTTCTCCAGTGAAAATGCGTTGTCGACCTTTTTTGCCAAGCCGCTTTgg                   >  2:4897627/1‑93 (MQ=255)
                                                                           aCGCTTTTCTGGGCAGG‑CTTTTTTTTGCAGGAAATCGATCTGTTTTTCTCCAGTGAAAATGCGTTGTCGACCTTTTTTGCCAAGCCGCTTTgg                   >  1:758544/1‑93 (MQ=255)
                                                                             gCTTTTCTGGGCAGG‑CTTTTTTTTGCAGGAAATCGATCTGTTTTTCTCCAGTGAAAATGCGTTGTCGACCTTTTTTGCCAAGCCGCTTTGGCt                 >  1:2352129/1‑93 (MQ=255)
                                                                             gCTTTTCTGGGCAGG‑CTTTTTTTTGCAGGAAATCGATCTGTTTTTCTCCAGTGAAAATGCGTTGTCGACCTTTTTTGCCAAGCCGCTTTGGCt                 <  1:5337871/93‑1 (MQ=255)
                                                                             gCTTTTCTGGGCAGG‑CTTTTTTTTGCAGGAAATCGATCTGTTTTTCTCCAGTGAAAATGCGTTGTCGACCTTTTTTGCCAAGCCGCTTTGGCt                 <  2:6699616/93‑1 (MQ=255)
                                                                             gCTTTTCTGGGCAGG‑CTTTTTTTTGCAGGAAATCGATCTGTTTTTCTCCAGTGAAAATGCGTTGTCGACCTTTTTTGCCAAGCCGCTTTGGCt                 >  2:4288126/1‑93 (MQ=255)
                                                                              cTTTTCTGGGCAGG‑CTTTTTTTTGCAGGAAATCGATCTGTTTTTCTCCAGTGAAAATGCGTTGTCGACCTTTTTTGCCAAGCCGCTTTGGCtt                >  2:383604/1‑93 (MQ=255)
                                                                              cTTTTCTGGGCAGG‑CTTTTTTTTGCAGGAAATCGATCTGTTTTTCTCCAGTGAAAATGCGTTGTCGACCTTTTTTGCCAAGCCGCTTTGGCtt                <  2:6514109/93‑1 (MQ=255)
                                                                               ttttCTGGGCAGG‑CTTTTTTTTGCAGGAAATCGATCTGTTTTTCTCCAGTGAAAATGCGTTGTCGACCTTTTTTGCCAAGCCGCTTTGGCTTc               <  2:344798/93‑1 (MQ=255)
                                                                                tttCTGGGCAGG‑CTTTTTTTTGCAGGAAATCGATCTGTTTTTCTCCAGTGAAAATGCGTTGTCGACCTTTTTTGCCAAGCCGCTTTGGCTTCg              <  2:533464/93‑1 (MQ=255)
                                                                                   cTGGGCAGG‑CTTTTTTTTGCAGGAAATCGATCTGTTTTTCTCCAGTGAAAATGCGTTGTCGCCCTTTTTTGCCAAGCCGCTTTGGCTTCGGtt           <  1:1929625/93‑1 (MQ=255)
                                                                                    tGGGCAGG‑CTTTTTTTTGCAGGAAATCGATCTGTTTTTCTCCAGTGAAAATGCGTTGTCGACCTTTTTTGCCAAGCCGCTTTGGCTTCGGTTa          <  2:3734414/93‑1 (MQ=255)
                                                                                    tGGGCAGG‑CTTTTTTTTGCAGGAAATCGATCTGTTTTTCTCCAGTGAAAATGCGTTGTCGACCTTTTTTGCCAAGCCGCTTTGGCTTCGGTTa          <  2:4169944/93‑1 (MQ=255)
                                                                                           g‑C‑TTTTTTTGCAGGAAATCGATCTGTTTTTCTCCAGTGAAAATGCGTTGTCGACCTTTTTTGCCAAGCCGCTTTGGCTTCGGTTATTCATAtt  <  1:3023570/93‑1 (MQ=255)
                                                                                              |                                                                                           
CGATAATTGCATTGCTCATTCTTTGTGGCGTAGTGTTGGCGATTAATCTGTTTTTTCTGCGCTTCTCCAAGCGGTACGCTTTTCTGGGCAGG‑C‑TTTTTTTGCAGGAAATCGATCTGTTTTTCTCCAGTGAAAATGCGTTGTCGACCTTTTTTGCCAAGCCGCTTTGGCTTCGGTTATTCATATT  >  NC_000913/1095667‑1095850

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑