BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,472,339	(C)_6→7	coding (95/861 nt)	ydbC →	putative dehydrogenase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,472,333	1	.	C	97.5%	512.6 / 5.0	165	coding (89/861 nt)	ydbC	putative dehydrogenase
Reads supporting (aligned to +/- strand): ref base . (1/3); new base C (88/71); total (89/76)
Fisher's exact test for biased strand distribution p-value = 3.30e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.00e-01

Only 100 of 183 total aligned reads displayed.

TGCGATGAGCAGCAATACATTTACTCTCGGTACAAAATCCGTTAACCGTCTTGGTTATGGCGCGATGCAACTGGCAGGTCCTGGAGTTTTT‑GG‑CCCCCCACGAGATCGCCACGTCGCTATAACCGTGCTGCGTGAGGCGCTGGCATTGGGCGTCAATCATATTGATACCAGCGACTTTT  >  NC_000913/1472241‑1472419

tGCGATGAGCAGCAATACATTTACTCTCGGTACAAAATCCGTTAACCGTCTTGGTTATGGCGCGATGCAACTGGCAGGTCCTGGAGTTTTT‑gg                                                                                         <  2:3802496/93‑1 (MQ=255)

   gATGAGCAGCAATACATTTACTCTCGGTACAAAATCCGTTAACCGTCTTGGTTATGGCGCGATGCAACTGGCAGGTCCTGGAGTTTTT‑GG‑ccc                                                                                     >  2:5564102/1‑93 (MQ=255)

   gATGAGCAGCAATACATTTACTCTCGGTACAAAATCCGTTAACCGTCTTGGTTATGGCGCGATGCAACTGGCAGGTCCTGGAGTTTTT‑GG‑ccc                                                                                     >  1:2310330/1‑93 (MQ=255)

    aTGAGCAGCAATACATTTACTCTCGGTACAAAATCCGTTAACCGTCTTGGTTATGGCGCGATGCAACTGGCAGGTCCTGGAGTTTTT‑GG‑cccc                                                                                    >  2:3435736/1‑93 (MQ=255)

    aTGAGCAGCAATACATTTACTCTCGGTACAAAATCCGTTAACCGTCTTGGCTATGGCGCGATGCAACTGGCAGGTCCTGGAGTTGTT‑GT‑cccc                                                                                    >  1:2146976/1‑93 (MQ=255)

     tGAGCAGCAATACATTTACTCTCGGTACAAAATCCGTTAACCGTCTTGGTTATGGCGCGATGCAACTGGCAGGTCCTGGAGTTTTT‑GG‑ccccc                                                                                   >  1:5971535/1‑93 (MQ=255)

     tGAGCAGCAATACATTTACTCTCGGTACAAAATCCGTTAACCGTCTTGGTTATGGCGCGATGCAACTGGCAGGTCCTGGAGTTTTT‑GG‑ccccc                                                                                   >  2:2984263/1‑93 (MQ=255)

      gAGCAGCAATACATTTACTCTCGGTACAAAATCCGTTAACCGTCTTGGTTATGGCGCGATGCAACTGGCAGGTCCTGGAGTTTTT‑GG‑cccccc                                                                                  <  2:3476193/93‑1 (MQ=255)

      gAGCAGCAATACATTTACTCTCGGTACAAAATCCGTTAACCGTCTTGGTTATGGCGCGATGCAACTGGCAGGTCCTGGAGTTTTT‑GG‑cccccc                                                                                  >  1:2894496/1‑93 (MQ=255)

             aaTACATTTACTCTCGGTACAAAATCCGTTAACCGTCTTGGTTATGGCGCGATGCAACTGGCAGGTCCTGGAGTTTTT‑GGCCCCCCCACgaga                                                                            >  2:782996/1‑93 (MQ=255)

              aTACATTTACTCTCGGTACAAAATCCGTTAACCGTCTTGGTTATGGCGCGATGCAACTGGCAGGTCCTGGAGTTTTT‑GGCCCCCCCACGAGAt                                                                           <  2:993078/93‑1 (MQ=255)

              aTACATTTACTCTCGGTACAAAATCCGTTAACCGTCTTGGTTATGGCGCGATGCAACTGGCAGGTCCTGGAGTTTTT‑GGCCCCCCCACGAGAt                                                                           <  1:98832/93‑1 (MQ=255)

              aTACATTTACTCTCGGTACAAAATCCGTTAACCGTCTTGGTTATGGCGCGATGCAACTGGCAGGTCCTGGAGTTTTT‑GGCCCCCCCACGAGAt                                                                           <  2:2686614/93‑1 (MQ=255)

              aTACATTTACTCTCGGTACAAAATCCGTTAACCGTCTTGGTTATGGCGCGATGCAACTGGCAGGTCCTGGAGTTTTT‑GGCCCCCCCACGAGAt                                                                           <  2:5690637/93‑1 (MQ=255)

                aCATTTACTCTCGGTACAAAATCCGTTAACCGTCTTGGTTATGGCGCGATGCAACTGGCAGGTCCTGGAGTTTTT‑GGCCCCCCCACGAGATCg                                                                         >  2:2224109/1‑93 (MQ=255)

                 cATTTACTCTCGGTACAAAATCCGTTAACCGTCTTGGTTATGGCGCGATGCAACTGGCAGGTCCTGGAGTTTTT‑GGCCCCCCCACGAGATCGc                                                                        >  2:4111687/1‑93 (MQ=255)

                  aTTTACTCTCGGTACAAAATCCGTTAACCGTCTTGGTTATGGCGCGATGCAACTGGCAGGTCCTGGAGTTTTT‑GGCCCCCCCACGAGCTCGcc                                                                       >  1:5394146/1‑93 (MQ=255)

                   tttACTCTCGGTACAAAATCCGTTAACCGTCTTGGTTATGGCGCGATGCAACTGGCAGGTCCTGGAGTTTTTAGC‑CCCCCCACGAGATCGccg                                                                      >  1:1989275/1‑92 (MQ=255)

                   tttACTCTCGGTACAAAATCCGTTAACCGTCTTGGTTATGGCGCGATGCAACTGGCAGGTCCTGGAGTTTTT‑GGCCCCCCCACGAGATCGccg                                                                      <  2:1205909/93‑2 (MQ=255)

                   tttACTCTCGGTACAAAATCCGTTAACCGTCTTGGTTATGGCGCGATGCAACTGGCAGGTCCTGGAGTTTTT‑GGCCCCCCCACGAGATCGccg                                                                      <  1:4728093/93‑2 (MQ=255)

                    ttACTCTCGGTACAAAATCCGTTAACCGTCTTGGTTATGGCGCGATGCAACTGGCAGGTCCTGGAGCTTTT‑GGCCCCCCCACGAGATCGccgc                                                                     >  2:6309477/1‑91 (MQ=255)

                     tACTCTCGGTACAAAATCCGTTAACCGTCTTGGTTATGGCGCGATGCAACTGGCAGGTCCTGGAGTTTTT‑GGCCCCCCCACGAGATCGCcgcg                                                                    >  1:2472525/1‑93 (MQ=255)

                       ctctCGGTACAAAATCCGTTAACCGTCTTGGTTATGGCGCGATGCAACTGGCAGGTCCTGGAGTTTTT‑GGCCCCCCCACGAGATCGCCGCGTc                                                                  >  2:1654893/1‑93 (MQ=255)

                        tctcGGTACAAAATCCGTTAACCGTCTTGGTTATGGCGCGATGCAACTGGCAGGTCCTGGAGTTTTT‑GGCCCCCCCACGAGATCGCCGCGTCg                                                                 >  1:1243644/1‑93 (MQ=255)

                         ctcGGTACAAAATCCGTTAACCGTCTTGGTTATGGCGCGATGCAACTGGCAGGTCCTGGAGTTTTT‑GGCCCCCCCACGAGATCGCCGCGTCGc                                                                <  1:5683984/93‑1 (MQ=255)

                             gTACAAAATCCGTTAACCGTCTTGGTTATGGCGCGATGCAACTGGCAGGTCCTGGAGTTTTT‑GGCCCCCCCACGAGATCGCCGCGTCGCtata                                                            <  2:5089491/93‑1 (MQ=255)

                                cAAAATCCGTTAACCGTCTTGGTTATGGCGCGATGCAACTGGCAGGTCCTGGAGTTTTT‑GGCCCCCCCACGAGATCGCCGCGTCGCTATAAcc                                                         >  1:1081609/1‑93 (MQ=255)

                                  aaaTCCGTTAACCGTCTTGGTTATGGCGCGATGCAACTGGCAGGTCCTGGAGTTTTT‑GGCCCCCCCACGAGATCGCCGCGTCGCTATAACCGt                                                       >  2:3139098/1‑93 (MQ=255)

                                  aaaTCCGTTAACCGTCTTGGTTATGGCGCGATGCAACTGGCAGGTCCTGGAGTTTTT‑GGCCCCCCCACGAGATCGCCGCGTCGCTATAACCGt                                                       <  2:4679303/93‑1 (MQ=255)

                                  aaaTCCGTTAACCGTCTTGGTTATGGCGCGATGCAACTGGCAGGTCCTGGAGTTTTT‑GGCCCCCCCACGAGATCGCCGCGTCGCTATAACCGt                                                       >  2:1965987/1‑93 (MQ=255)

                                  aaaTCCGTTAACCGTCTTGGTTATGGCGCGATGCAACTGGCAGGTCCTGGAGTTTTT‑GGCCCCCCCACGAGATCGCCGCGTCGCTATAACCGt                                                       >  2:2929193/1‑93 (MQ=255)

                                    aTCCGTTAACCGTCTTGGTTATGGCGCGATGCAACTGGCAGGTCCTGGAGTTTTT‑GGCCCCCCCACGAGATCGCCGCGTCGCTATAACCGtgc                                                     <  1:2436016/93‑1 (MQ=255)

                                    aTCCGTTAACCGTCTTGGTTATGGCGCGATGCAACTGGCAGGTCCTGGAGTTTTT‑GGCCCCCCCACGAGATCGCCGCGTCGCTATAACCGtgc                                                     <  2:5801759/93‑1 (MQ=255)

                                    aTCCGTTAACCGTCTTGGTTATGGCGCGATGCAACTGGCAGGTCCTGGAGTTTTT‑GGCCCCCCCACGAGATCGCCGCGTCGCTATAACCGtgc                                                     >  1:787009/1‑93 (MQ=255)

                                    aTCCGTTAACCGTCTTGGTTATGGCGCGATGCAACTGGCAGGTCCTGGAGTTTTT‑GGCCCCCCCACGAGATCGCCGCGTCGCTATAACCGtgc                                                     <  2:2874402/93‑1 (MQ=255)

                                       cGTTAACCGTCTTGGTTATGGCGCGATGCAACTGGCAGGTCCTGGAGTTTTTAGC‑CCCCCCACGAGATCGCAGCGTCGCCATGACCGtgctgc                                                  >  1:3143584/1‑93 (MQ=255)

                                        gTTAACCGTCTTGGTTATGGCGCGATGCAACTGGCAGGTCCTGGAGTTTTT‑GGCCCCCCCACGAGATCGCCGCGTCGCTATAACCGTGCTGCg                                                 >  2:3660740/1‑93 (MQ=255)

                                         ttAACCGTCTTGGTTATGGCGCGATGCAACTGGCAGGTCCTGGAGTTTTT‑GGCCCCCCCACGAGATCGCCGCGTCGCTATAACCGTGCTGCGt                                                >  2:5908790/1‑93 (MQ=255)

                                         ttAACCGTCTTGGTTATGGCGCGATGCAACTGGCAGGTCCTGGAGTTTTT‑GGCCCCCCCACGAGATCGCCGCGTCGCTATAACCGTGCTGCGt                                                <  2:5058113/93‑1 (MQ=255)

                                         ttAACCGTCTTGGTTATGGCGCGAGGCAACTGGCAGGTCCTGGAGTTTTT‑GGCCCCCCCACGAGATCGCCGCGTCGCTATAACCGTGCTGCGt                                                <  2:1841090/93‑1 (MQ=255)

                                          tAACCGTCTTGGTTATGGCGCGATGCAACTGGCAGGTCCTGGAGTTTTT‑GGCCCCCCCACGAGATCGCCGCGTCGCTATAACCGTGCTGCGTg                                               <  1:1606510/93‑1 (MQ=255)

                                           acccGTCTTGGTTATCGCGCGATGCAACTGGCAGGTCCTGGAGTTTTT‑GGCCCCCCCACGAGATCGCCGCGTCGCTATAACCGTGCTGCGTGa                                              <  1:3866865/91‑1 (MQ=255)

                                           aaCCGTCTTGGTTATGGCGCGATGCAACTGGCAGGTCCTGGAGTTTTT‑GGCCCCCCCACGAGATCGCCGCGTCGCTATACCCGTGCTGCGTgg                                              >  2:393831/1‑92 (MQ=255)

                                           aaCCGTCTTGGTTATGGCGCGATGCAACTGGCAGGTCCTGGAGTTTTT‑GGCCCCCCCACGAGATCGCCGCGTCGCTATAACCGTGCTGCGTGa                                              >  1:5606398/1‑93 (MQ=255)

                                             ccGTCTTGGTTATGGCGCGATGCAACTGGCAGGTCCTGGAGTTTTT‑GGCCCCCCCACGAGATCGCCGCGTCGCTATAACCGTGCTGCGTGAgg                                            <  2:3781229/93‑1 (MQ=255)

                                               gTCTTGGTTATGGCGCGATGCAACTGGCAGGTCCTGGAGTTTTT‑GGCCCCCCCACGAGATCGCCGCGTCGCTATAACCGTGCTGCGTGAGgcg                                          >  2:2401547/1‑93 (MQ=255)

                                               gTCTTGGTTATGGCGCGATGCAACTGGCAGGTCCTGGAGTTTTT‑GGCCCCCCCACGAGATCGCCGCGTCGCTATAACCGTGCTGCGTGAGgcg                                          >  1:3168566/1‑93 (MQ=255)

                                               gTCTTGGTTATGGCGCGATGCAACTGGCAGGTCCTGGAGTTTTT‑GGCCCCCCCACGAGATCGCCGCGTCGCTATAACCGTGCTGCGTGAGgcg                                          >  1:2993622/1‑93 (MQ=255)

                                               gTCTTGGTTATGGCGCGATGCAACTGGCAGGTCCTGGAGTTTTT‑GGCCCCCCCACGAGATCGCCGCGTCGCTATAACCGTGCTGCGTGAGgcg                                          >  2:1617564/1‑93 (MQ=255)

                                               gTCTTGGTTATGGCGCGATGCAACTGGCAGGTCCTGGAGTTTTT‑GGCCCCCCCACGAGATCGCCGCGTCGCTATAACCGTGCTGCGTGAGgcg                                          >  1:4187448/1‑93 (MQ=255)

                                               gTCTTGGTTATGGCGCGATGCAACTGGCAGGTCCTGGAGTTTTT‑GGCCCCCCCACGAGATCGCCGCGTCGCTATAACCGTGCTGCGCGAGgcg                                          >  1:2474622/1‑93 (MQ=255)

                                                  ttGGTTATGGCGCGATGCAACTGGCAGGTCCTGGAGTTTTT‑GGCCCCCCCACGAGATCGCCGCGTCGCTATAACCGTGCTGCGTGAGGCGCTg                                       <  2:564896/93‑1 (MQ=255)

                                                  ttGGTTATGGCGCGATGCAACTGGCAGGTCCTGGAGTTTTT‑GGCCCCCCCACGAGATCGCCGCGTCGCTATAACCGTGCTGCGTGAGGCGCTg                                       <  2:6012799/93‑1 (MQ=255)

                                                  ttGGTTATGGCGCGATGCAACTGGCAGGTCCTGGAGTTTTT‑GGCCCCCCCACGAGATCGCCGCGTCGCTATAACCGTGCTGCGTGAGGCGCTg                                       <  1:4936424/93‑1 (MQ=255)

                                                   tGGTTATGGCGCGATGCAACTGGCAGGTCCTGGAGGTTTT‑GGCCCCCCCACGAGATCGCCGCGTCGCTATAACCGTGCTGCGTGAGGCGCTgg                                      >  2:830302/1‑93 (MQ=255)

                                                     gatATGGCGCGATGCAACTGGCAGGTCCTGGAGTTTTT‑GGCCCCCCCACGAGATCGCCGCGTCGCTATAACCGTGCTGCGTGAGGCGCTGGCa                                    >  1:4204866/3‑93 (MQ=255)

                                                     gTTATGGCGCGATGCAACTGGCAGGTCCTGGAGTTTTT‑GGCCCCCCCACGAGATCGCCGCGTCGCTATAACCGTGCTGCGTGAGGCGCTGGCa                                    >  1:3959708/1‑93 (MQ=255)

                                                     gTTATGGCGCGATGCAACTGGCAGGTCCTGGAGTTTTT‑GGCCCCCCCACGAGATCGCCGCGTCGCTATAACCGTGCTGCGTGAGGCGCTGGCa                                    >  2:6200683/1‑93 (MQ=255)

                                                     gTTATGGCGCGATGCAACTGGCAGGTCCTGGAGTTTTT‑GGCCCCCCCACGAGATCGCCGCGTCGCTATAACCGTGCTGCGTGAGGCGCTGGCa                                    >  1:4761782/1‑93 (MQ=255)

                                                      ttATGGCGCGATGCAACTGGCAGGTCCTGGAGTTTTT‑GGCCCCCCCACGAGATCGCCGCGTCGCTATAACCGTGCTGCGTGAGGCGCTGGCAt                                   <  1:860298/93‑1 (MQ=255)

                                                       tATGGCGCGATGCAACTGGCAGGTCCTGGAGTTTTT‑GGCCCCCCCACGAGATCGCCGCGTCGCTATAACCGTGCTGCGTGATGCGCTGGCCtt                                  >  1:1076723/1‑93 (MQ=255)

                                                       tATGGCGCGATGCAACTGGCAGGTCCTGGAGTTTTT‑GGCCCCCCCACGAGATCGCCGCGTCGCTATAACCGTGCTGCGTGAGGCGCTGGCAtt                                  <  1:1954782/93‑1 (MQ=255)

                                                       tATGGCGCGATGCAACTGGCAGGTCCTGGAGTTTTT‑GGCCCCCCCACGAGATAGCCGCGTCGCTATAACCGTGCTGCGTGAGGCGCTGGCAtt                                  <  2:6169128/93‑1 (MQ=255)

                                                        aTGGCGCGATGCAACTGGCAGGTCCTGGAGTTTTT‑GGCCCCCCCACGAGATCGCCGCGTCGCTATAACCGTGCTGCGTGAGGCGCTGGCATTg                                 >  2:6531542/1‑93 (MQ=255)

                                                            cgcgATGCAACTGGCAGGTCCTGGAGTTTTT‑GGCCCCCCCACGAGATCGCCGCGTCGCTATAACCGTGCTGCGTGAGGCGCTGGCATTGGGCg                             <  1:4436359/93‑1 (MQ=255)

                                                            cgcgATGCAACTGGCAGGTCCTGGAGTTTTT‑GGCCCCCCCACGAGATCGCCGCGTCGCTATAACCGTGCTGCGTGAGGCGCTGGCATTGGGCg                             <  2:137516/93‑1 (MQ=255)

                                                            cgcgATGCAACTGGCAGGTCCTGGAGTTTTT‑GGCCCCCCCACGAGATCGCCGCGTCGCTATAACCGTGCTGCGTGAGGCGCTGGCATTGGGCg                             <  1:3666158/93‑1 (MQ=255)

                                                              cgATGCAACCGGCAGGGCCTGGTGTTTTT‑GGCCCCCCCACGAGATCGCCGCGTCGCTATAACCGTGCTGCGTGAGGCGCTGGCATTGGGCGTc                           <  2:4678395/93‑1 (MQ=255)

                                                               gATGCAACTGGCAGGTCCTGGAGTTTTT‑GGCCCCCCCACGAGATCGCCGCGTCGCTATAACCGTGCTGCGTGAGGCGCTGGCATTGGGCGTCa                          >  1:1133062/1‑93 (MQ=255)

                                                               gATGCAACTGGCAGGTCCTGGAGTTTTT‑GGCCCCCCCACGAGATCGCCGCGTCGCTATAACCGTGCTGCGTGAGGCGCTGGCATTGGGCGTCa                          >  2:1663064/1‑93 (MQ=255)

                                                                aTGCAACTGGCAGGACCTGGAGCTTTT‑GGCCCCCCCACGAGATCGCCGCGTCGCTATAACCGTGCTGCGTGAGGCGCTGGCATTGGGCGTCaa                         >  1:5748936/1‑93 (MQ=255)

                                                                  gCAACTGGCAGGTCCTGGAGTTTTT‑GGCCCCCCCACGAGATCGCCGCGTCGCTATAACCGTGCTGCGTGAGGCGCTGGCATTGGGCGTCAATc                       <  2:4781727/93‑1 (MQ=255)

                                                                    aaCTGGCAGGTCCTGGAGTTTTT‑GGCCCCCCCACGAGATCGCAGCGTCGCTATAACCGTGCTGCGTGAGGCGCTGGCATTGGGCGTCAATCat                     >  2:3572146/1‑93 (MQ=255)

                                                                     aCTGGCAGGTCCTGGAGTTTTT‑GGCCCCCCCACGAGATCGCCGCGTCGCTATAACCGTGCTGCGTGAGGCGCTGGCATTGGGCGTCAATCata                    <  2:4041475/93‑1 (MQ=255)

                                                                     aCTGGCAGGTCCTGGAGTTTTT‑GGCCCCCCCACGAGATCGCCGCGTCGCTATAACCGTGCTGCGTGAGGCGCTGGCATTGGGCGTCAATCata                    >  2:5150190/1‑93 (MQ=255)

                                                                       tGGCAGGTCCTGGAGTTTTT‑GGCCCCCCCACGAGATCGCCGCGTCGCTATAACCGTGCTGCGTGAGGCGCTGGCATTGGGCGTCAATCATAtt                  <  2:3337390/93‑1 (MQ=255)

                                                                       tGGCAGGTCCTGGAGTTTTT‑GGCCCCCCCACGAGATCGCCGCGTCGCTATAACCGTGCTGCGTGAGGCGCTGGCATTGGGCGTCAATCATAtt                  <  1:1854729/93‑1 (MQ=255)

                                                                       tGGCAGGTCCTGGAGTTTTT‑GGCCCCCCCACGAGATCGCCGCGTCGCTATAACCGTGCTGCGTGAGGCGCTGGCATTGGGCGTCAATCATAtt                  >  2:5168477/1‑93 (MQ=255)

                                                                         gCAGGTCCTGGAGTTTTT‑GGCCCCCCCACGAGATCGCCGCGTCGCTATAACCGTGCTGCGTGAGGCGCTGGCATTGGGCGTCAATCATATTGa                <  1:4688992/93‑1 (MQ=255)

                                                                          cAGGTCCTGGAGTTTTT‑GGCCCCCCCACGAGATCGCCGCGTCGCTATAACCGTGCTGCGTGAGGCGCTGGCATTGGGCGTCAATCATATTGAt               >  2:5257684/1‑93 (MQ=255)

                                                                            ggTCCTGGAGTTTTT‑GGCCCCCCCACGAGATCGCCGCGTCGCTATAACCGTGCTGCGTGAGGCGCTGGCATTGGGCGTCAATCATATTGATAc             >  1:6616635/1‑93 (MQ=255)

                                                                               ccTGGAGTTTTT‑GGCCCCCCCACGAGATCGCCGCGTCGCTATAACCGTGCTGCGTGAGGCGCTGGCATTGGGCGTCAATCATATTGATACCAg          <  1:428223/93‑1 (MQ=255)

                                                                               ccTGGAGTTTTT‑GGCCCCCCCACGAGATCGCCGCGTCGCTATAACCGTGCTGCGTGAGGCGCTGGCATTGGGCGTCAATCATATTGATACCAg          <  1:6558209/93‑1 (MQ=255)

                                                                               ccTGGAGTTTTT‑GGCCCCCCCACGAGATCGCCGCGTCGCTATAACCGTGCTGCGTGAGGCGCTGGCATTGGGCGTCAATCATATTGATACCAg          >  2:3848046/1‑93 (MQ=255)

                                                                                cTGGAGTTTTT‑GGCCCCCCCACGAGATCGCCGCGTCGCTATAACCGTGCTGCGTGAGGCGCTGGCATTGGGCGTCAATCATATTGATACCAGc         >  2:4973790/1‑93 (MQ=255)

                                                                                cTGGAGTTTTT‑GGCCCCCCCACGAGATCGCCGCGTCGCTATAACCGTGCTGCGTGAGGCGCTGGCATTGGGCGTCAATCATATTGATACCAGc         >  1:867502/1‑93 (MQ=255)

                                                                                 tGGAGTTTTT‑GGCCCCCCCACGAGATCGCCGCGTCGCTATAACCGTGCTGCGTGAGGCGCTGGCGTTGGGCGTCAATCATATTGATACCAGCg        >  2:3627656/1‑93 (MQ=255)

                                                                                  ggAGTTTTT‑GGCCCCCCCACGAGATCGCCGCGTCGCTATAACCGTGCTGCGTGAGGCGCTGGCATTGGGCGTCAATCATATTGATACCAGCGa       >  2:5016440/1‑93 (MQ=255)

                                                                                   gAGTTTTT‑GGCCCCCCCACGAGATCGCCGCGTCGCTATAACCGTGCTGCGTGAGGCGCTGGCATTGGGCGTCAATCATATTGATACCAGCGAc      >  1:5504101/1‑93 (MQ=255)

                                                                                   gAGTTTTT‑GGCCCCCCCACGAGATCGCCGCGTCGCTATAACCGTGCTGCGTGAGGCGCTGGCATTGGGCGTCAATCATATTGATACCAGCGAc      <  2:1750613/93‑1 (MQ=255)

                                                                                   gAGTTTTT‑GGCCCCCCCACGAGATCGCCGCGTCGCTATAACCGTGCTGCGTGAGGCGCTGGCATTGGGCGTCAATCATATTGATACCAGCGAc      <  2:1862119/93‑1 (MQ=255)

                                                                                    aGTTTTT‑GGCCCCCCCACGAGATCGCCGCGTCGCTATAACCGTGCTGCGTGAGGCGCTGGCATTGGGCGTCAATCATATTGATACCAGCGACt     >  2:4507561/1‑93 (MQ=255)

                                                                                     gTTTTT‑GGCCCCCCCACGAGATCGCCGCGTCGCTATAACCGTGCTGCGTGAGGCGCTGGCATTGGGCGTCAATCATATTGATACCAGCGACtt    >  2:5889969/1‑93 (MQ=255)

                                                                                     gTTTTT‑GGCCCCCCCACGAGATCGCCGCGTCGCTATAACCGTGCTGCGTGAGGCGCTGGCATTGGGCGTCAATCATATTGATACCAGCGACtt    <  2:2125876/93‑1 (MQ=255)

                                                                                      ttttt‑GGCCCCCCCACGAGATCGCCGCGTCGCTATAACCGTGCTGCGTGAGGCGCTGGCATTGGGCGTCAATCATATTGATACCAGCGACttt   >  2:1925909/1‑93 (MQ=255)

                                                                                      ttttt‑GGCCCCCCCACGAGATCGCCGCGTCGCTATAACCGTGCTGCGTGAGGCGCTGGCATTGGGCGTCAATCATATTGATACCAGCGACttt   >  1:3302840/1‑93 (MQ=255)

                                                                                      ttttt‑GGCCCCCCCACGAGATCGCCGCGTCGCTATAACCGTGCTGCGTGAGGCGCTGGCATTGGGCGTCAATCATATTGATACCAGCGACttt   >  2:3094983/1‑93 (MQ=255)

                                                                                      ttttg‑GC‑CCCCCCACGAGATCGCCGCGTCGCTATAACCGTGCTGCGTGAGGCGCTGGCATTGGGCGTCAATCATATTGATACCAGCGACtttt  <  1:3899898/93‑1 (MQ=255)

                                                                                      ttttg‑GC‑CCCCCCACGAGATCGCCGCGTCGCTATAACCGTGCTGCGTGAGGCGCTGGCATTGGGCGTCAATCATATTGATACCAGCGACtttt  <  1:6471893/93‑1 (MQ=255)

                                                                                       tttt‑GGCCCCCCCACGAGATCGCCGCGTCGCTATAACCGTGCTGCGTGAGGCGCTGGCATTGGGCGTCAATCATATTGATACCAGCGACtttt  >  1:2656785/1‑93 (MQ=255)

TGCGATGAGCAGCAATACATTTACTCTCGGTACAAAATCCGTTAACCGTCTTGGTTATGGCGCGATGCAACTGGCAGGTCCTGGAGTTTTT‑GG‑CCCCCCACGAGATCGCCACGTCGCTATAACCGTGCTGCGTGAGGCGCTGGCATTGGGCGTCAATCATATTGATACCAGCGACTTTT  >  NC_000913/1472241‑1472419

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 19 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`