BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,594,797	(C)_6→7	coding (1314/3978 nt)	ydeK ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,594,791	1	.	C	97.4%	477.8 / 5.2	151	coding (1320/3978 nt)	ydeK	hypothetical protein
Reads supporting (aligned to +/- strand): ref base . (2/2); new base C (79/68); total (81/70)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.43e-01

Only 100 of 184 total aligned reads displayed.

GCGATACGAACTTACCGCCATCGCTAATATTGAGGTTTCCGACGCCCGCAACACCAATGGTGGTATAGACATTCTTATTGACCTCCCACATT‑CCCC‑CCGTGGTGATGCTGACATTCCCGACGCCGGTTTCTCCATAACCGAGTGAACTTCCTCCGTTGCTGACAACAAGCCCCTGATT  >  NC_000913/1594700‑1594877

gCGATACGAACTTACCGCCATCGCTAATATTGAGGTTTCCGACGCCCGCAACACCAATGGTGGTATAGACATTCTTATTGACCTCCCACATT‑c                                                                                        <  2:4250325/93‑1 (MQ=255)

  gATACGAACTTACCGCCATCGCTAATATTGAGGTTTCCGACGCCCGCAACACCAATGGTGGTATAGACATTCTTATTGACCTCCCACATT‑ccc                                                                                      >  2:3625860/1‑93 (MQ=255)

  gATACGAACTTACCGCCATCGCTAATATTGAGGTTTCCGACGCCCGCAACACCAATGGTGGTATAGACATTCTTATTGACCTCCCACATT‑ccc                                                                                      >  1:2415468/1‑93 (MQ=255)

  gATACGAACTTACCGCCATCGCTAATATTGAGGTTTCCGACGCCCGCAACACCAATGGTGGTATAGACATTCTTATTGACCTCCCACATT‑ccc                                                                                      >  1:2070178/1‑93 (MQ=255)

  gATACGAACTTACCGCCATCGCTAATATTGAGGTTTCCGACGCCCGCAACACCAATGGTGGTATAGACATTCTTATTGACCTCCCACATT‑ccc                                                                                      >  2:2399596/1‑93 (MQ=255)

  gATACGAACTTACCGCCATCGCTAATATTGAGGTTTCCGACGCCCGCAACACCAATGGTGGTATAGACATTCTTATTGACCTCCCACATT‑ccc                                                                                      >  2:2816221/1‑93 (MQ=255)

  gATACGAACTTACCGCCATCGCTAATATTGAGGTTTCCGACGCCCGCAACACCAATGGTGGTATAGACATTCTTATTGACCTCCCACATT‑cac                                                                                      >  2:3127029/1‑91 (MQ=255)

    tACGAACTTACCGCCATCGCTAATATTGAGGTTTCCGACGCCCGCAACACCAATGGTGGTATAGACATTCTTATTGACCTCCCACATT‑cccc‑c                                                                                   >  1:3578888/1‑93 (MQ=255)

      cGAACTTACCGCCATCGCTAATATTGAGGTTTCCGACGCCCGCAACACCAATGGTGGTATAGACATTCTTATTGACCTCCCACATT‑cccc‑ccc                                                                                 <  2:6660428/93‑2 (MQ=255)

       gAACTTACCGCCATCGCTAATATTGAGGTTTCCGACGCCCGCAACACCAATGGTGGTATAGACATTCTTATTGACCTCCCACATT‑cccc‑cccg                                                                                <  2:2901425/93‑3 (MQ=255)

        aaCTTCCCGCCATCGCTAATATTGAGGTTTCCGACGCCCGCAACACCAATGGTGGTATAGACATTCTTATTGACCTCCCACATT‑cccc‑cccgt                                                                               <  2:2480937/93‑4 (MQ=255)

        aaCTTACCGCCATCGCTAATATTGAGGTTTCCGACGCCCGCAACACCAATGGTGGTATAGACATTCTTATTGACCTCCCACATT‑cccc‑cccgt                                                                               <  2:4952673/93‑4 (MQ=255)

        aaCTTACCGCCATCGCTAATATTGAGGTTTCCGACGCCCGCAACACCAATGGTGGTATAGACATTCTTATTGACCTCCCACATT‑cccc‑cccgt                                                                               >  2:6315237/1‑90 (MQ=255)

        aaCTTACCGCCATCGCTAATATTGAGGTTTCCGACGCCCGCAACACCAATGGTGGTATAGACATTCTTATTGACCTCCCACATT‑cccc‑cccgt                                                                               <  1:4985454/93‑4 (MQ=255)

        aaCTTACCGCCATCGCTAATATTGAGGTTTCCGACGCCCGCAACACCAATGGTGGTATAGACATTCTTATTGACCTCCCACATT‑cccc‑cccgg                                                                               >  2:1772242/1‑90 (MQ=255)

         aCTTACCGCCATCGCTAATATTGAGGTTTCCGACGCCCGCAACACCAATGGTGGTATAGACATTCTTATTGACCTCCCACATT‑cccc‑cccgtg                                                                              <  2:2853663/93‑5 (MQ=255)

         aCTTACCGCCATCGCTAATATTGAGGTTTCCGACGCCCGCAACACCAATGGTGGTATAGACATTCTTATTGACCTCCCACATT‑cccc‑cccgtg                                                                              >  1:1002243/1‑89 (MQ=255)

         aCTTACCGCCATCGCTAATATTGAGGTTTCCGACGCCCGCAACACCAATGGTGGTATAGACATTCTTATTGACCTCCCACATT‑cccc‑cccgtg                                                                              >  1:5627399/1‑89 (MQ=255)

         aCTTACCGCCATCGCTAATATTGAGGTTTCCGACGCCCGCAACACCAATGGTGGTATAGACATTCTTATTGACCTCCCACATT‑cccc‑cccgtg                                                                              <  1:3465592/93‑5 (MQ=255)

            tACCGCCATCGCTAATATTGAGGTTTCCGACGCCCGCAACACCAATGGTGGTATAGACATTCTTATTGACCTCCCACATTCCCCC‑CCgtggtg                                                                            >  1:1242022/1‑93 (MQ=255)

             aCCGCCATCGCTAATATTGAGGTTTCCGACGCCCGCAACACCAATGGTGGTATAGACATTCTTATTGACCTCCCACATTCCCCC‑CCGTGGTGa                                                                           >  2:1921186/1‑93 (MQ=255)

               cGCCATCGCTAATATTGAGGTTTCCGACGCCCGCAACACCAATGGTGGTATAGACATTCTTATTGACCTCCCACATTCCCCC‑CCGTGGTGATg                                                                         >  2:1820947/1‑93 (MQ=255)

               cGCCATCGCTAATATTGAGGTTTCCGACGCCCGCAACACCAATGGTGGTATAGACATTCTTATTGACCTCCCACATTCCCCC‑CCGTGGTGATg                                                                         >  1:4325689/1‑93 (MQ=255)

                gCCATCGCTAATATTGAGGTTTCCGACGCCCGCAACACCAATGGTGGTATAGACATTCTTATTGACCTCCCACATTCCCCC‑CCGTGGTGATGc                                                                        <  1:1968/93‑1 (MQ=255)

                 ccATCGCTAATATTGAGGTTTCCGACGCCCGCAACACCAATGGTGGTATAGACATTCTTATTGACCTCCCACATTCCCCC‑CCGTGGTGATGCt                                                                       <  2:336416/93‑1 (MQ=255)

                 ccATCGCTAATATTGAGGTTTCCGACGCCCGCAACACCAATGGTGGTATAGACATTCTTATTGACCTCCCACATTCCCCC‑CCGTGGTGATGCt                                                                       >  2:4517445/1‑93 (MQ=255)

                  cATCGCTAATATTGAGGTTTCCGACGCCCGCAACACCAATGGTGGTATAGACATTCTTATTGACCTCCCACATTCCCCC‑CCGTGGTGATGCTg                                                                      >  2:4044197/1‑93 (MQ=255)

                  cATCGCTAATATTGAGGTTTCCGACGCCCGCAACACCAATGGTGGTATAGACATTCTTATTGACCTCCCACATTCCCCC‑CCGTGGTGATGCTg                                                                      >  1:894714/1‑93 (MQ=255)

                  cATCGCTAATATTGAGGTTTCCGACGCCCGCAACACCAATGGTGGTATAGACATTCTTATTGACCTCCCACATTCCCCC‑CCGTGGTGATGCTg                                                                      >  1:4551453/1‑93 (MQ=255)

                  cATCGCTAATATTGAGGTTTCCGACGCCCGCAACACCAATGGTGGTATAGACATTCTTATTGACCGCCCACATTCCCCC‑CCGTGGTGATGCTg                                                                      <  1:690312/93‑1 (MQ=255)

                    tCGCTAATATTGAGGTTTCCGACGCCCGCAACACCAATGGTGGTATAGACATTCTTATTGACCTCCCACATTCCCCC‑CCGTGGTGATGCTGAc                                                                    <  2:4272045/93‑1 (MQ=255)

                    tCGCTAATATTGAGGTTTCCGACGCCCGCAACACCAATGGTGGTATAGACATTCTTATTGACCTCCCACATTCCCCC‑CCGTGGTGATGCTGAc                                                                    >  1:5942068/1‑93 (MQ=255)

                      gCTAATATTGAGGTTTCCGACGCCCGCAACACCAATGGTGGTATAGACATTCTTATTGACCTCCCACATTCCCCC‑CCGTGGTGATGCTGACAt                                                                  >  1:4955124/1‑93 (MQ=255)

                       cTAATATTGAGGTTTCCGACGCCCGCAACACCAATGGTGGTATAGACATTCTTATTGACCTCCCACATTCCCCC‑CCGTGGTGATGCTGACAtt                                                                 >  2:381671/1‑93 (MQ=255)

                       cTAATATTGAGGTTTCCGACGCCCGCAACACCAATGGTGGTATAGACATTCTTATTGACCTCCCACATTCCCCC‑CCGTGGTGATGCTGACAtt                                                                 >  1:4402452/1‑93 (MQ=255)

                       cTAATATTGAGGTTTCCGACGCCCGCAACACCAATGGTGGTATAGACATTCTTATTGACCTCCCACATTCCCCC‑CCGTGGTGATGCTGACAtt                                                                 >  1:2718727/1‑93 (MQ=255)

                        tAATATTGAGGTTTCCGACGCCCGCAACACCAATGGTGGTATAGACATTCTTATTGACCTCCCACATTCCCCC‑CCGTGGTGATGCTGACATTc                                                                <  1:987869/93‑1 (MQ=255)

                        tAATATTGAGGTTTCCGACGCCCGCAACACCAATGGTGGTATAGACATTCTTATTGACCTCCCACATTCCCCC‑CCGTGGTGATGCTGACATTc                                                                >  1:1743544/1‑93 (MQ=255)

                        tAATATTGAGGTTTCCGACGCCCGCAACACCAATGGTGGTATAGACATTCTTATTGACCTCCCACATTCCCCC‑CCGTGGTGATGCTGACATTc                                                                >  1:2945001/1‑93 (MQ=255)

                         tatatTGAGGTTTCCGACGCCCGCAACACCAATGGTGGTATAGACATTCTTATTGACCTCCCACATTCCCCC‑CCGTGGTGATGCTGACATTcc                                                               <  2:1681585/92‑1 (MQ=255)

                         aaTATTGAGGTTTCCGACGCCCGCAACACCAATGGTGGTATAGACATTCTTATTGACCTCCCACATTCCCCC‑CCGTGGTGATGCTGACATTcc                                                               <  2:6164241/93‑1 (MQ=255)

                           tatTGAGGTTTCCGACGCCCGCAACACCAATGGTGGTATAGACATTCTTATTGACCTCCCACATTCCCCC‑CCGTGGTGATGCTGACATTCCCg                                                             >  2:348608/1‑93 (MQ=255)

                           tatTGAGGTTTCCGACGCCCGCAACACCAATGGTGGTATAGACATTCTTATTGACCTCCCACATTCCCCC‑CCGTGGTGATGATGACATTCACg                                                             >  1:135512/1‑93 (MQ=255)

                               gAGGTTTCCGACGCCCGCAACACCAATGGTGGTATAGACATTCTTATTGACCTCCCACATTCCCCC‑CCGTGGTGATGCTGACATTCCcgccgc                                                         >  1:1202547/1‑93 (MQ=255)

                               gAGGTTTCCGACGCCCGCAACACCAATGGTGGTATAGACATTCTTATTGACCTCCCACATTCCCCC‑CCGTGGTGATGCTGACATTCCCGACGc                                                         <  2:1394871/93‑1 (MQ=255)

                                aGGTTTCCGACGCCCGCAACACCAATGGTGGTATAGACATTCTTATTGACCTCCCACATTCCCCC‑CCGTGGTGATGCTGACATTCCCGACGcc                                                        <  1:1576254/93‑1 (MQ=255)

                                 ggTTTCCGACGCCCGCAACACCAATGGTGGTATAGACATTCTTATTGACCTCCCACATTCCCCC‑CCGTGGTGATGCTGACATTCCCGACGCCg                                                       >  1:3813634/1‑93 (MQ=255)

                                 ggTTTCCGACGCCCGCAACACCAATGGTGGTATAGACATTCTTATTGACCTCCCACATTCCCCC‑CCGTGGTGATGCTGACATTCCCGACGCCg                                                       <  2:3781099/93‑1 (MQ=255)

                                 ggTTTCCGACGCCCGCAACACCAATGGTGGTATAGACATTCTTATTGACCTCCCACATT‑CCCCGCCGTGGTGATGCTGACATTCCCGACGCCg                                                       >  2:4085463/1‑93 (MQ=255)

                                   tttCCGACGCCCGCAACACCAATGGTGGTATAGACATTCTTATTGACCTCCCCCATTCCCCC‑CCGTGGTGATGCTGACATTCCCGACGCCGGt                                                     <  1:4536204/93‑1 (MQ=255)

                                    ttCCGACGCCCGCAACACCAATGGTGGTATAGACATTCTTATTGACCTCCCACATTCCCCC‑CCGTGGTGATGCTGACATTCCCGACGCCGGtt                                                    <  2:6402402/93‑1 (MQ=255)

                                     tCCGACGCCCGCAACACCAATGGTGGTATAGACATTCTTATTGACCTCCCACATTCCCCC‑CCGTGGTGATGCTGACATTCCCGACGCCGGttt                                                   <  1:3593661/93‑1 (MQ=255)

                                     tCCGACGCCCGCAACACCAATGGTGGTATAGACATTCTTATTGACCTCCCACATTCCCCC‑CCGTGGTGATGCTGACATTCCCGACGCCGGttt                                                   <  2:2445860/93‑1 (MQ=255)

                                      ccGACGCCCGCAACACCAATGGTGGTATAGACATTCTTATTGACCTCCCACATTCCCCC‑CCGTGGTGATGCTGACATTCCCGACGCCGGTTtc                                                  <  1:2948276/93‑1 (MQ=255)

                                      ccGACGCCCGCAACACCAATGGTGGTATAGACATTCTTATTGACCTCCCACATTCCCCC‑CCGTGGTGATGCTGACATTCCCGACGCCGGTTtc                                                  <  1:2800842/93‑1 (MQ=255)

                                       cGACGCCCGCAACACCAATGGTGGTATAGACATTCTTATTGACCTCCCACATTCCCCC‑CCGTGGTGATGCTGACATTCCCGACGCCGGTTtct                                                 <  2:6001113/93‑1 (MQ=255)

                                        gACGCCCGCAACACCAATGGTGGTATAGACATTCTTATTGACCTCCCACATTCCCCC‑CCGTGGTGATGCTGACATTCCCGACGCCGGTTtctc                                                >  1:581094/1‑93 (MQ=255)

                                        gACGCCCGCAACACCAATGGTGGGATAGACATTCTTATTGACCTCCCACATTCCCCC‑CCGTGGTGATGCTGACATTCCCGACGCCGGTTtctc                                                >  2:3948714/1‑93 (MQ=255)

                                         aCGCCCGCAACACCAATGGTGGTATAGACATTCTTATTGACCTCCCACATTCCCCC‑CCGTGGTGATGCTGACATTCCCGACGCCGGTTTCTcc                                               >  1:3856101/1‑93 (MQ=255)

                                          cGCCCGCAACACCAATGGTGGTATAGACATTCTTATTGACCTCCCACATTCCCCC‑CCGTGGTGATGCTGACATTCCCGACGCCGGTTTCTCCa                                              <  1:1444671/93‑1 (MQ=255)

                                             ccGCAACACCAATGGTGGTATAGACATTCTTATTGACCTCCCACATTCCCCC‑CCGTGGTGATGCTGACATTCCCGACGCCGGTTTCTCCATaa                                           >  2:2197133/1‑93 (MQ=255)

                                              cGCAACACCAATGGTGGTATAGACATTCTTATTGACCTCCCACATTCCCCC‑CCGTGGTGATGCTGACATTCCCGACGCCGGTTTCTCCATAAc                                          <  2:1731537/93‑1 (MQ=255)

                                                  acacCAATGGTGGTATAGACATTCTTATTGACCTCCCACATTCCCCC‑CCGTGGTGATGCTGACATTCCCGACGCCGGATTCTCCATAAACGAg                                      >  1:3938049/1‑93 (MQ=255)

                                                   cacCAATGGTGGGATAGACATTCTTATTGACCTCCCACATTCCCCC‑CCGTGGTGATGCTGAGATTCCCGACGCGGGGTTCTTCATAAGCGAGt                                     >  1:3790699/1‑93 (MQ=255)

                                                    acCAATGGTGGTATAGACATTCTTATTGACCTCCCACATTCCCCC‑CCGTGGTGATGCTGACATTCCCGACGCCGGTTTCTCCATAACCGAGTg                                    >  1:3974254/1‑93 (MQ=255)

                                                     ccAATGGTGGTATAGACATTCTCATTACCCTCCCACATTCCCCC‑CCGTGGTGATGCTGAAATTCACGACGCCGGTTTCTCCATAACCGAGTGa                                   <  1:3695014/93‑1 (MQ=255)

                                                       aaTGGTGGTATAGACATTCTTATTGACCTCCCACATTCCCCC‑CCGTGGTGATGCTGACATTCCCGACGCCGGTTTCTCCATAACCGAGTGAAc                                 <  2:710583/93‑1 (MQ=255)

                                                       aaTGGTGGTATAGACATTCTTATTGACCTCCCACATTCCCCC‑CCGTGGTGATGCTGACATTCCCGACGCCGGTTTCTCCATAACCGAGTGAAc                                 <  2:1469807/93‑1 (MQ=255)

                                                        aTGGTGGTATAGACATTCTTATTGACCTCCCACATTCCCCC‑CCGTGGTGATGCTGACATTCCCGACGCCGGTTTCTCCATAACCGAGTGAACt                                <  1:5506170/93‑1 (MQ=255)

                                                        aTGGTGCTATAGACATTCTTATTGCCCTCCCACATTCCCCC‑CCGTGGTGATGCTGACATTCCCGACGCCGGTTTCTCCATAACCGAGTGAACt                                <  1:4873637/93‑1 (MQ=255)

                                                          ggtggtATAGACATTCTTATTGACCTCCCACATTCCCCC‑CCGTGGTGATGCTGACATTCCCGACGCCGGTTTCTCCATAACCGAGTGAACTtc                              <  1:542247/93‑1 (MQ=255)

                                                            tggtATAGACATTCTTATTGACCTCCCACATTCCCCC‑CCGTGGTGATGCTGACATTCCCGACGCCGGTTTCTCCATAACCGAGTGAACTtcct                            <  1:4720532/93‑1 (MQ=255)

                                                             ggtATAGACATTCTTATTGACCTCCCACATTCCCCC‑CCGTGGTGATGCTGACATTCCCGACGCCGGTTTCTCCATAACCGAGTGAACTtcctc                           <  1:927040/93‑1 (MQ=255)

                                                             ggtATAGACATTCTTATTGACCTCCCACATTCCCCC‑CCGTGGTGATGCTGACATTCCCGACGCCGGTTTCTCCATAACCGAGTGAACTtcctc                           >  1:915506/1‑93 (MQ=255)

                                                               tataGACATTCTTATTGACCTCCCACATTCCCCC‑CCGTGGTGATGCTGACATTCCCGACGCCGGTTTCTCCATAACCGAGTGAACTTCCTCCg                         <  2:5228091/93‑1 (MQ=255)

                                                               tataGACATTCTTATTGACCTCCCACATTCCCCC‑CCGTGGTGATGCTGACATTCCCGACGCCGGTTTCTCCATAACCGAGTGAACTTCCTCCg                         >  2:6715036/1‑93 (MQ=255)

                                                                ataGACATTCTTATTGACCTCCCACATT‑CCCC‑CCGTGGTGATGCTGACATTCCCGACGCCGGTTTCTCCATAACCGAGTGAACTTCCTCCGtt                       <  1:4511562/93‑1 (MQ=255)

                                                                 taGACATTCTTATTGACCTCCCACATTCCCCC‑CCGTGGTGATGCTGACATTCCCGCCGCCGGTTTCTCCATAACCGAGTGAACTTCCTCCGtt                       >  2:267402/1‑93 (MQ=255)

                                                                 taGACATTCTTATTGACCTCCCACATTCCCCC‑CCGTGGTGATGCTGACATTCCCGACGCCGGTTTCTCCATAACCGAGTGAACTTCCTCCGtt                       >  1:1073254/1‑93 (MQ=255)

                                                                  aGACATTCTTATTGACCTCCCACATTCCCCC‑CCGTGGTGATGCTGACATTCCCGACGCCGGTTTCTCCATAACCGAGTGAACTTCCTCCGTTg                      >  1:4224835/1‑93 (MQ=255)

                                                                   gACATTCTTATTGACCTCCCACATTCCCCC‑CCGTGGTGATGCTGACATTCCCGACGCCGGTTTCTCCATAACCGAGTGAACTTCCTCCGTTGc                     <  2:2750495/93‑1 (MQ=255)

                                                                   gACATTCTTATTGACCTCCCACATTCCCCC‑CCGTGGTGATGCTGACATTCCCGACGCCGGTTTCTCCATAACCGAGTGAACTTCCTCCGTTGc                     <  2:5047941/93‑1 (MQ=255)

                                                                    aCATTCTTATTGACCTCCCACATTCCCCC‑CCGTGGTGATGCTGACATTCCCGACGCCGGTTTCTCCATAACCGAGTGAACTTCCTCCGTTGCt                    >  2:5497510/1‑93 (MQ=255)

                                                                    aCATTCTTATTGACCTCCCACATTCCCCC‑CCGTGGTGATGCTGACATTCCCGACGCCGGTTTCTCCATAACCGAGTGAACTTCCTCCGTTGCt                    <  1:2502715/93‑1 (MQ=255)

                                                                      aTTCTTATTGACCTCCCACATT‑CCCC‑CCGTGGTGATGCTGACATTCCCGACGCCGGTTTCTCCATAACCGAGTGAACTTCCTCCGTTGCTGac                 >  2:4071074/1‑93 (MQ=255)

                                                                       ttCTTATTGACCTCCCACATTCCCCC‑CCGTGGTGATGCTGACATTCCCGACGCCGGTTTCTCCATAACCGAGTGAACTTCCTCCGTTGCTGac                 >  1:6461963/1‑93 (MQ=255)

                                                                         cTTATTGACCTCCCCCATTCCCCC‑CCGTGGTGATGCTGACATTCCCGACGCCGGTTTCTCCATAACCGAGTGAACTTCCTCCGTTGCTGacaa               <  2:628984/93‑1 (MQ=255)

                                                                         cTTATTGACCTCCCACATTCCCCC‑CCGTGGTGATGCTGACATTCCCGACGCCGGTTTCTCCATAACCGAGTGAACTTCCTCCGTTGCTGacaa               >  1:5920910/1‑93 (MQ=255)

                                                                           tATTGACCTCCCACATTCCCCC‑CCGTGGTGATGCTGACATTCCCGACGCCGGTTTCTCCATAACCGAGTGAACTTCCTCCGTTGCTGacaaca             <  1:3314847/93‑1 (MQ=255)

                                                                            aTTGACCTCCCACATTCCCCC‑CCGTGGTGATGCTGACATTCCCGACGCCGGTTTCTCCATAACCGAGTGAACTTCCTCCGTTGCTGAcaacaa            >  1:3038043/1‑93 (MQ=255)

                                                                             ttGACCTCCCACATTCCCCC‑CCGTGGTGATGCTGACATTCCCGACGCCGGTTTCTCCATAACCGAGTGAACTTCCTCCGTTGCTGACAACAAg           >  2:5353153/1‑93 (MQ=255)

                                                                             ttGACCTCCCACATTCCCCC‑CCGTGGTGATGCTGACATTCCCGACGCCGGTTTCTCCATAACCGAGTGAACTTCCTCCGTTGCTGACAACAAg           >  2:1349957/1‑93 (MQ=255)

                                                                              tGACCTCCCACATTCCCCC‑CCGTGGTGATGCTGACATTCCCGACGCCGGTTTCTCCATAACCGAGTGAACTTCCTCCGTTGCTGACAACAAGc          <  2:5715437/93‑1 (MQ=255)

                                                                                 ccTCCCACATTCCCCC‑CCGTGGTGATGCTGACATTCCCGACGCCGGTTTCTCCATAACCGAGTGAACTTCCTCCGTTGCTGACAACAAGcccc       >  1:610857/1‑93 (MQ=255)

                                                                                    cccACATTCCCCC‑CCGTGGTGATGCTGACATTCCCGACGCCGGTTTCTCCATAACCGAGTGAACTTCCTCCGTTGCTGACAACAAGCCCCTGa    >  2:5441846/1‑93 (MQ=255)

                                                                                     ccACATTCCCCC‑CCGTGGTGATGCTGACATTCCCGACGCCGGTTTCTCCATAACCGAGTGAACTTCCTCCGTTGCTGACAACAAGCCCCTGAt   >  2:3686824/1‑93 (MQ=255)

                                                                                     ccACATTCCCCC‑CCGTGGTGATGCTGACATTCCCGACGCCGGTTTCTCCATAACCGAGTGAACTTCCTCCGTTGCTGACAACAAGCCCCTGAt   <  1:5851909/93‑1 (MQ=255)

                                                                                      cacaTTCCCCC‑CCGTGGTGATGCTGACATTCCCGACGCCGGTTTCTCCATAACCGAGTGAACTTCCTCCGTTGCTGACAACAAGCCCCTGAtt  <  1:5583844/93‑1 (MQ=255)

                                                                                      cacaTTCCCCC‑CCGTGGTGATGCTGACATTCCCGACGCCGGTTTCTCCATAACCGAGTGAACTTCCTCCGTTGCTGACAACAAGCCCCTGAtt  >  1:671122/1‑93 (MQ=255)

                                                                                      cacaTTCCCCC‑CCGTGGTGATGCTGACATTCCCGACGCCGGTTTCTCCATAACCGAGTGAACTTCCTCCGTTGCTGACAACAAGCCCCTGAtt  <  2:822785/93‑1 (MQ=255)

GCGATACGAACTTACCGCCATCGCTAATATTGAGGTTTCCGACGCCCGCAACACCAATGGTGGTATAGACATTCTTATTGACCTCCCACATT‑CCCC‑CCGTGGTGATGCTGACATTCCCGACGCCGGTTTCTCCATAACCGAGTGAACTTCCTCCGTTGCTGACAACAAGCCCCTGATT  >  NC_000913/1594700‑1594877

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 19 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`