Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,184,181	(T)5→6	intergenic (+200/‑22)	ygiL → / → yi215	putative fimbrial‑like protein/IS2 hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,184,176	1	.	T	100.0%	199.0 / NA	60	intergenic (+195/‑27)	ygiL/yi215	putative fimbrial‑like protein/IS2 hypothetical protein
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base T (41/19);  total (41/19)

AAAATATTTATTGTTCATTATCCGTTATTAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAG‑TTTTTAGACTAGTCATTGGAGAACA  >  NC_000913/3184084‑3184201                                                                                              |                          aaaaTATTTATTGTTCATTATCCGTTATTAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAg                            >  2:3276065/1‑93 (MQ=255) aaaaTATTTATTGTTCATTATCCGTTATTAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAg                            <  2:1931574/93‑1 (MQ=255)  aaaTATTTATTGTTCATTATCCGTTATTAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAG‑t                          <  2:987372/93‑1 (MQ=255)  aaaTATTTATTGTTCATTATCCGTTATTAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAG‑t                          >  1:1593502/1‑93 (MQ=255)  aaaTATTTATTGTTCATTATCCGTTATTAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAG‑t                          <  2:5182491/93‑1 (MQ=255)   aaTATTTATTGTTCATTATCCGTTATTAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAGATAAGTGATAGTCTTAATACTAG‑tt                         >  1:4410328/1‑93 (MQ=255)   aaTATTTATTGTTCATTATCCGTTATTAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAG‑tt                         <  2:3332199/93‑1 (MQ=255)   aaTATTTATTGTTCATTATCCGTTATTAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAG‑tt                         >  1:2865501/1‑93 (MQ=255)    atatTTATTGTTCATTATCCGTTATTAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAG‑ttt                        <  1:848808/93‑1 (MQ=255)     tatttattGTTCATTATCCGTTATTAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAG‑tttt                       >  1:1405135/1‑93 (MQ=255)     tatttattGTTCATTATCCGTTATTAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAG‑tttt                       >  1:173108/1‑93 (MQ=255)     tatttattGTTCATTATCCGTTATTAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAG‑tttt                       <  2:2121841/93‑1 (MQ=255)     tatttattGTTCATTATCCGTTATTAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAG‑tttt                       >  2:577809/1‑93 (MQ=255)      atttattGTTCATTATCCGTTATTAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAG‑ttttt                      >  1:5304863/1‑93 (MQ=255)      atttattGTTCATTATCCGTTATTAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAG‑ttttt                      >  1:4078616/1‑93 (MQ=255)      atttattGTTCATTATCCGTTATTAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAG‑ttttt                      <  1:3938232/93‑1 (MQ=255)      atttattGTTCATTATCCGTTATTAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAG‑ttttt                      <  1:14534/93‑1 (MQ=255)       tttattGTTCATTATCCGTTATTAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAG‑tttttt                     >  2:3698364/1‑92 (MQ=255)       tttattGTTCATTATCCGTTATTAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAG‑tttttt                     >  2:6546254/1‑92 (MQ=255)       tttattGTTCATTATCCGTTATTAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAG‑tttttt                     >  2:4416984/1‑92 (MQ=255)       tttattGTTCATTATCCGTTATTAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAG‑tttttt                     >  2:4757892/1‑92 (MQ=255)       tttattGTTCATTATCCGTTATTAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAG‑tttttt                     >  2:6718827/1‑92 (MQ=255)       tttattGTTCATTATCCGTTATTAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAG‑tttttt                     <  1:5554014/93‑2 (MQ=255)       tttattGTTCATTATCCGTTATTAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAG‑attttt                     >  1:1134958/1‑92 (MQ=255)        ttattGTTCATTATCCGTTATTAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAG‑tttttta                    <  1:6672799/93‑3 (MQ=255)         tattGTTCATTATCCGTTATTAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAG‑ttttttag                   <  1:2697023/93‑4 (MQ=255)         tattGTTCATTATCCGTTATTAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAG‑ttttttag                   >  1:714943/1‑90 (MQ=255)          attGTTCATTATCCGTTATTAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAG‑ttttttaga                  >  1:1811903/1‑89 (MQ=255)          attGTTCATTATCCGTTATTAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAG‑ttttttaga                  <  2:1629587/93‑5 (MQ=255)              ttCATTATCCGTTCTTAGACTTGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGCCTTAATACTAGTTTTTTAGACTAg               >  1:3063926/1‑93 (MQ=14)              ttCATTATCCGTTATTAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAGTTTTTTAGACTAg               >  1:214037/1‑93 (MQ=255)              ttCATTATCCGTTATTAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAGTTTTTTAGACTAg               >  2:224215/1‑93 (MQ=255)              ttCATTATCCGTTATTAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAGTTTTTTAGACTAg               >  2:5660906/1‑93 (MQ=255)              ttCATTATCCGTTATTAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAGTTTTTTAGACTAg               >  2:6041722/1‑93 (MQ=255)              ttCATTATCCGTTATTAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAGTTTTTTAGACTAg               >  2:1252510/1‑93 (MQ=255)               tCATTATCCGTTATTAGACTGGCCCCCTGAATCTCCAGACAACCGATATCACTTAAATAAGTGATAGTCTTAATACTAGTTTTTTAGACTAGt              >  2:2956058/1‑93 (MQ=255)                cATTATCCGTTATTAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAGTTTTTTAGACTAGTc             <  1:2093134/93‑1 (MQ=255)                cATTATCCGTTATTAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAGTTTTTTAGACTAGTc             >  2:5561505/1‑93 (MQ=255)                cATTATCCGTTATTAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAGTTTTTTAGACTAGTc             <  2:4359374/93‑1 (MQ=255)                 aTTATCCGTTATTAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAGTTTTTTAGACTAGTCa            >  2:5941636/1‑93 (MQ=255)                 aTTATCCGTTATTAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAGTTTTTTAGACTAGTCa            >  2:6729745/1‑93 (MQ=255)                 aTTATCCGTTATTAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAGTTTTTTAGACTAGTCa            >  1:4478245/1‑93 (MQ=255)                 aTTATCCGTTATTAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAGTTTTTTAGACTAGTCa            >  1:5257022/1‑93 (MQ=255)                 aTTATCCGTTATTAGACTAGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAGTTTTTTAGACTAGTCa            >  2:4721581/1‑93 (MQ=255)                  ttATCCGTTATTAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAGTTTTTTAGACTAGTCAt           >  2:2439413/1‑93 (MQ=255)                  ttATCCGTTATTAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAGTTTTTTAGACTAGTCAt           <  2:3155233/93‑1 (MQ=255)                  ttATCCGTTATTAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAGTTTTTTAGACTAGTCAt           >  2:962286/1‑93 (MQ=255)                  ttATCCGTTATTAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAGTTTTTTAGACTAGTCAt           >  2:1816002/1‑93 (MQ=255)                  ttATCCGTTATTAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAGTTTTTTAGACTAGTCAt           >  2:887143/1‑93 (MQ=255)                  ttATCCGTTATTAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAGTTTTTTAGACTAGTCAt           >  1:3635130/1‑93 (MQ=255)                   tATCCGTTATTAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAGTTTTTTAGACTAGTCAtt          <  2:4044023/93‑1 (MQ=255)                   tATCCGTTATTAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAGTTTTTTAGACTAGTCAtt          >  2:4901939/1‑93 (MQ=255)                   tATCCGTTATTAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAGTTTTTTAGACTAGTCAtt          >  2:4951905/1‑93 (MQ=255)                   tATCCGTTATTAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAGTTTTTTAGACTAGTCAtt          >  2:199521/1‑93 (MQ=255)                   tATCCGTTATTAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAGTTTTTTAGACTAGTCAtt          >  1:4349111/1‑93 (MQ=255)                   tATCCGTTATTAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAGTTTTTTAGACTAGTCAtt          <  2:1192532/93‑1 (MQ=255)                    aTCCGTTATTAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAGTTTTTTAGACTAGTCATTg         >  2:4222118/1‑93 (MQ=255)                    aTCCGTTATTAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAGTTTTTTAGACTAGTCATTg         <  2:5022629/93‑1 (MQ=255)                    aTCCGTTATTAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAGTTTTTTAGACTAGTCATTg         <  1:36306/93‑1 (MQ=255)                    aTCCGTTATTAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAGTTTTTTAGACTAGTCATTg         <  2:5022628/93‑1 (MQ=255)                    aTCCGTTATTAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAGTTTTTTAGACTAGTCATTg         <  1:687290/93‑1 (MQ=255)                    aTCCGTTATTAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAGTTTTTTAGACTAGTCATTg         >  1:3976479/1‑93 (MQ=255)                     tCCGTTATTAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAGTTTTTTAGACTAGTCATTgg        >  2:5570217/1‑93 (MQ=255)                     tCCGTTATTAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAGTTTTTTAGACTAGTCATTgg        >  1:6388288/1‑93 (MQ=255)                     tCCGTTATTAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAGTTTTTTAGACTAGTCATTgg        >  2:5529863/1‑93 (MQ=255)                     tCCGTTATTAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAGTTTTTTAGACTAGTCATTgg        <  2:6472381/93‑1 (MQ=255)                     tCCGTTATTAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAGTTTTTTAGACTAGTCATTgg        >  2:4837363/1‑93 (MQ=255)                     tCCGTTATTAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAGTTTTTTAGACTAGTCATTgg        >  2:6255657/1‑93 (MQ=255)                     tCCGTTATTAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAGTTTTTTAGACTAGTCATTgg        >  1:2257172/1‑93 (MQ=255)                     tCCGTTATTAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTCAAATAAGTGATAGTCTTAATACTAGTTTTTTAGACTAGTCATTgg        >  1:2410730/1‑93 (MQ=255)                      ccGTTATTAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAGTTTTTTAGACTAGTCATTGga       >  2:6325455/1‑93 (MQ=255)                      ccGTTATTAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAGTTTTTTAGACTAGTCATTGga       >  2:5831823/1‑93 (MQ=255)                      ccGTTATTAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAGTTTTTTAGACTAGTCATTGga       <  1:4134077/93‑1 (MQ=255)                      ccGTTATTAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAGTTTTTTAGACTAGTCATTGga       <  2:4452472/93‑1 (MQ=255)                       cGTTATTAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAGTTTTTTAGACTAGTCATTGgag      >  2:1311224/1‑93 (MQ=255)                        gTTATTAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAGTTTTTTAGACTAGTCATTGgaga     >  1:3799433/1‑93 (MQ=255)                        gTTATTAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAGTTTTTTAGACTAGTCATTGgaga     <  2:984026/93‑1 (MQ=255)                        gTTATTAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAGTTTTTTAGACTAGTCATTGgaga     >  1:1249581/1‑93 (MQ=255)                        gTTATTAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAGTTTTTTAGACTAGTCATTGgaga     >  2:4520896/1‑93 (MQ=255)                         ttattaGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAGTTTTTTAGACTAGTCATTGGAGaa    <  2:6598675/93‑1 (MQ=16)                         ttattaGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAGTTTTTTAGACTAGTCATTGGAGaa    <  1:857341/93‑1 (MQ=16)                         ttattaGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAGTTTTTTAGACTAGTCATTGGAGaa    >  2:1800655/1‑93 (MQ=16)                          tattaGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAGTTTTTTAGACTAGTCATTGGAGAAc   >  1:4796969/1‑93 (MQ=12)                          tattaGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAGTTTTTTAGACTAGTCATTGGAGAAc   <  2:3640146/93‑1 (MQ=12)                           attaGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAGTTTTTTAGACTAGTCATTGGAGAACa  <  1:4663296/93‑1 (MQ=9)                           attaGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAGTTTTTTAGACTAGTCATTGGAGAACa  >  1:780574/1‑93 (MQ=9)                           attaGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAGTTTTTTAGACTAGTCATTGGAGAACa  <  2:6724483/93‑1 (MQ=9)                           attaGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAGTTTTTTAGACTAGTCATTGGAGAACa  >  1:887942/1‑93 (MQ=9)                           attaGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAGTTTTTTAGACTAGTCATTGGAGAACa  >  2:4280166/1‑93 (MQ=9)                           attaGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAGTTTTTTAGACTAGTCATGGGAGAACa  <  1:6572118/93‑1 (MQ=255)                                                                                              |                          AAAATATTTATTGTTCATTATCCGTTATTAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAG‑TTTTTAGACTAGTCATTGGAGAACA  >  NC_000913/3184084‑3184201

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑