BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
MC JC	NC_000913	4,474,849	20 bp→GTATTTCCCGGCATCTTTATAGCGATAGCA	intergenic (+24/+2)	yjgL → / ← argI	hypothetical protein/ornithine carbamoyltransferase 1

Missing coverage evidence...
			seq id	start	end	size	←reads	reads→	gene	description
*	*	÷	NC_000913	4474849	4474868	20	221 [0]	[0] 257	yjgL/argI	hypothetical protein/ornithine carbamoyltransferase 1

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913	= 4474848	0 (0.000)	164 (0.930) +GTATTTCCCGGCATCTTTATAGCGATAGCA	81/124	0.5	100%	intergenic (+23/+22)	yjgL/argI	hypothetical protein/ornithine carbamoyltransferase 1
*	?	NC_000913	4474869 =	0 (0.000)	164 (0.930) +GTATTTCCCGGCATCTTTATAGCGATAGCA	81/124	0.5	100%	intergenic (+44/+1)	yjgL/argI	hypothetical protein/ornithine carbamoyltransferase 1

Only 100 of 221 total aligned reads displayed.

AATCCACCGGAAAATTTACGTATAGCAATAGAACACTTTGGGTGGAAGAATAGACCTATCACTGCAT‑AAATAATGTGATGCCGGGATGGTTTCCGCATCTTATAGCGATCAGATTATTTACTGAGCGTCGCGACCATCACCGCTTTGATAGTATGCATACGGTTTTCCGCCTGATCAAAAACAA  >  NC_000913/4474757‑4474940

aaTCCACCGGAAAATTTACGTATAGCAATAGAACACTTTGGGTGGAAGAATAGACCTATCACTGCATAAAATAATGTGATGCCGGGATGGttt                                                                                              >  2:301310/1‑93 (MQ=255)

aaTCCACCGGAAAATTTACGTATAGCAATAGAACACTTTGGGTGGAAGAATAGACCTATCACTGCATAAAATAATGTGATGCCGGGATGGttt                                                                                              <  2:1094245/93‑1 (MQ=255)

 aTCCACCGGAAAATTTACGTATAGCAATAGAACACTTTGGGTGGAAGAATAGGCCTATCATGGCATAAAATAATGTGATGCCGGGCTGGTTTg                                                                                             >  1:3025965‑M1/1‑92 (MQ=255)

 aTCCACCGGAAAATTTACGTATAGCAATAGAACACTTTGGGTGGAAGAATAGACCTATCACTGCATAAAATAATGTGATGCCGGGATGGCTTg                                                                                             >  1:3508279‑M1/1‑92 (MQ=255)

  tCCACCGGAAAATTTACGTATAGCAATAGAACACTTTGGGTGGAAGAATAGACCTATCACTGCATAAAATAATGTGATGCCGGGATGGTTTgt                                                                                            >  2:5821026‑M1/1‑91 (MQ=255)

   ccACCGGAAAATTTACGTATAGCAATAGAACACTTTGGGTGGAAGAATAGACCTATCACTGCATAAAATAATGTGATGCCGGGATGGTTTgta                                                                                           >  1:3356028‑M1/1‑90 (MQ=255)

   ccACCGGAAAATTTACGTATAGCAATAGAACACTTTGGGTGGAAGAATAGACCTATCACTGCATAAAATAATGTGATGCCGGGATGGTTTgta                                                                                           <  1:4467053‑M1/93‑4 (MQ=255)

       cGGAAAATTTACGTATAGCAATAGAACACTTTGGGTGGAAGAATAGACCTATCACTGCATAAAATAATGTGATGCCGGGATGGTTTgtatttc                                                                                       <  1:5217804‑M1/93‑8 (MQ=255)

        ggAAAATTTACGTATAGCAATAGAACACTTTGGGTGGAAGAATAGACCTATCACTGCATAAAATAATGTGATGCCGGGATGGTTTgtatttcc                                                                                      <  1:5872213‑M1/93‑9 (MQ=255)

        ggAAAATTTACGTATAGCAATAGAACACTTTGGGTGGAAGAATAGACCTATCACTGCATAAAATAATGTGATGCCGGGATGGTTTgtatttcc                                                                                      >  1:5825474‑M1/1‑85 (MQ=255)

          aaaaTTTACGTATAGCAATAGAACACTTTGGGTGGAAGAATAGACCTATCACTGCATAAAATAATGTGATGCCGGGATGGTTTgtatttcccg                                                                                    >  1:5653241‑M1/1‑83 (MQ=255)

           aaaTTTACGTATAGCAATAGAACACTTTGGGTGGAAGAATAGACCTATCACTGCATAAAATAATGTGATGCCGGGATGGTTTgtatttcccgg                                                                                   >  1:3924108‑M1/1‑82 (MQ=255)

            aaTTTACGTATAGCAATAGAACACTTTGGGTGGAAGAATAGACCTATCACTGCATAAAATAATGTGATGCCGGGATGGTTTgtatttcccggc                                                                                  >  2:201147‑M1/1‑81 (MQ=255)

            aaTTTACGTATAGCAATAGAACACTTTGGGTGGAAGAATAGACCTATCACTGCATAAAATAATGTGATGCCGGGATGGTTTgtatttcccggc                                                                                  <  1:386652‑M1/93‑13 (MQ=255)

              tttACGTATAGCAATAGAACACTTTGGGTGGGAGAATAGACCTATCACTGCATAAAATAATGTGATGCCGGGATGGTTTgtatttccctgcat                                                                                >  1:413388‑M1/1‑79 (MQ=255)

                 aCGTATAGCAATAGAACACTTTGGGTGGAAGAATAGACCTATCACTGCATAAAATAATGTGATGCCGGGATGGTTTgtatttcccggcatctt                                                                             >  1:5020136‑M1/1‑76 (MQ=255)

                 aCGTATAGCAATAGAACACTTTGGGTGGAAGAATAGACCTATCACTGCATAAAATAATGTGATGCCGGGATGGTTTgtatttcccggcatctt                                                                             >  1:4008771‑M1/1‑76 (MQ=255)

                 aCGTATAGCAATAGAACACTTTGGGTGGAAGAATAGACCTATCACTGCATAAAATAATGTGATGCCGGGATGGTTTgtatttcccggcatctt                                                                             >  1:1300502‑M1/1‑76 (MQ=255)

                   gTATAGCAATAGAACACTTTGGGTGGAAGAATAGACCTATCACTGCATAAAATAATGTGATGCCGGGATGGTTTgtatttcccggcatcttta                                                                           <  2:6314657‑M1/93‑20 (MQ=255)

                     ataGCAATAGAACACTTTGGGTGGAAGAATAGACCTATCACTGCATAAAATAATGTGATGCCGGGATGGTTTgtatttcccggcatctttata                                                                         <  2:2483006‑M1/93‑22 (MQ=255)

                      taGCAATAGAACACTTTGGGTGGAAGAATAGACCTATCACTGCATAAAATAATGTGATGCCGGGATGGTTTgtatttcccggcatctttatag                                                                        >  1:1698029‑M1/1‑71 (MQ=255)

                        gCAATAGAACACTTTGGGTGGAAGAATAGACCTATCACTGCATAAAATAATGTGATGCCGGGATGGTTTgtatttcccggcatctttatagcg                                                                      >  1:6632630‑M1/1‑69 (MQ=255)

                           aTAGAACACTTTGGGTGGAAGAATAGACCTATCACTGCATAAAATAATGTGATGCCGGGATGGTTTgtatttcccggcatctttatagcgata                                                                   >  2:2244512‑M1/1‑66 (MQ=255)

                           aTAGAACACTTTGGGTGGAAGAATAGACCTATCACTGCATAAAATAATGTGATGCCGGGATGGTTTgtatttcccggcatctttatagcgata                                                                   <  1:6679325‑M1/93‑28 (MQ=255)

                            tAGAACACTTTGGGTGGAAGAATAGACCTATCACTGCATAAAATAATGTGATGCCGGGATGGTTTgtatttcccggcatctttatagcgatag                                                                  >  2:4369887‑M1/1‑65 (MQ=255)

                             aGAACACTTTGGGTGGAAGAATAGACCTATCACTGCATAAAATAATGTGATGCCGGGATGGTTTgtatttcccggcatctttatagcgatagc                                                                 >  2:162904‑M1/1‑64 (MQ=255)

                              gAACACTTTGGGTGGAAGAATAGACCTATCACTGCATAAAATAATGTGATGCCGGGATGGTTTgtatttcccggcatctttatagcgatagca                                                                >  2:3971897‑M1/1‑63 (MQ=255)

                               aaCACTTTGGGTGGAAGAATAGACCTATCACTGCATAAAATAATGTGATGCCGGGATGGTTTgtatttcccggcatctttatagcgatagcaa                                                               >  1:800570‑M1/1‑62 (MQ=255)

                               aaCACTTTGGGTGGAAGAATAGACCTATCACTGCATAAAATAATGTGATGCCGGGATGGTTTgtatttcccggcatctttatagcgatagcaa                                                               <  2:2211151‑M1/93‑32 (MQ=255)

                               aaCACTTTGGGTGGAAGAATAGACCTATCACTGCATAAAATAATGTGATGCCGGGATGGTTTgtatttcccggcatctttatagcgatagcaa                                                               >  2:5353577‑M1/1‑62 (MQ=255)

                                acacTTTGGGTGGAAGAATAGACCTATCACTGCATAAAATAATGTGATGCCGGGATGGTTTgtatttcccggcatctttatagcgatagcaat                                                              <  1:2753225‑M1/93‑33 (MQ=255)

                                   cTTTGGGTGGAAGAATAGACCTATCACTGCATAAAATAATGTGATGCCGGGATGGTTTgtatttcccggcatctttatagcgatagcaattat                                                           >  2:6583799‑M1/1‑58 (MQ=255)

                                   cTTTGGGGGGAAGAATAGACCTATCACTGCATAAAATAATGGGATGCCGGGGTGGTTTgtatttcccggcatctttatagcgatagcaattat                                                           >  1:1765140‑M1/1‑58 (MQ=255)

                                       gggTGGAAGAATAGACCTATCACTGCATAAAATAATGTGATGCCGGGATGGTTTgtatttcccggcatctttatagcgatagcaattatttac                                                       >  2:2049327‑M1/1‑54 (MQ=255)

                                           ggAAGAATAGACCTATCACTGCATAAAATAATGTGATGCCGGGATGGTTTgtatttcccggcatctttatagcgatagcaattatttactgag                                                   >  2:6334247‑M1/1‑50 (MQ=255)

                                           ggAAGAATAGACCTATCACTGCATAAAATAATGTGATGCCGGGATGGTTTgtatttcccggcatctttatagcgatagcaattatttactgag                                                   >  1:1964122‑M1/1‑50 (MQ=255)

                                           ggAAGAATAGACCTATCACTGCATAAAATAATGTGATGCCGGGATGGTTTgtatttcccggcatctttatagcgatagcaattatttactgag                                                   <  2:4181421‑M1/93‑44 (MQ=255)

                                           ggAAGAATAGACCTATCACTGCATAAAATAATGTGATGCCGGGATGGTTTgtatttcccggcatctttatagcgatagcaattatttactgag                                                   <  1:6047453‑M1/93‑44 (MQ=255)

                                           ggAAGAATAGACCTATCACTGCATAAAATAATGTGATGCCGGGATGGTTTgtatttcccggcatctttatagcgatagcaattatttactgag                                                   >  1:6206934‑M1/1‑50 (MQ=255)

                                            gaagaaTAGACCTATCACTGCATAAAATAATGTGATGCCGGGATGGTTTgtatttcccggcatctttatagcgatagcaattatttactgagt                                                  >  2:1541974‑M1/1‑49 (MQ=255)

                                            gaagaaTAGACCTATCACTGCATAAAATAATGTGATGCCGGGATGGTTTgtatttcccggcatctttatagcgatagcaattatttactgagt                                                  <  2:4245162‑M1/93‑45 (MQ=255)

                                              agaaTAGACCTATCACTGCATAAAATAATGTGATGCCGGGATGGTTTgtatttcccggcatctttatagcgatagcaattatttactgagtgt                                                >  2:3550093‑M1/1‑47 (MQ=255)

                                              agaaTAGACCTATCACTGCATAAAATAATGTGATGCCGGGATGGTTTgtatttcccggcatctttatagcgatagcaattatttactgagtgt                                                >  2:1136167‑M1/1‑47 (MQ=255)

                                              agaaTAGACCTATCACTGCATAAAATAATGTGATGCCGGGATGGTTTgtatttcccggcatctttatagcgatagcaattatttactgagtgt                                                >  1:1887733‑M1/1‑47 (MQ=255)

                                              agaaTAGACCTATCACTGCATAAAATAATGTGATGCCGGGATGGTTTgtatttcccggcatctttatagcgatagcaattatttactgagtgt                                                <  1:4207600‑M1/93‑47 (MQ=255)

                                               gaaTAGACCTATCACTGCATAAAATAATGTGATGCCGGGATGGTTTgtatttcccggcatctttatagcgatagcaattatttactgagtgtc                                               <  2:4650649‑M1/93‑48 (MQ=255)

                                                 aTAGACCTATCACTGCATAAAATAATGTGATGCCGGGATGGGTTgtatttcccggcatctttatagcgatagcaattatttactgagtgtcgc                                             >  1:206186‑M1/1‑44 (MQ=255)

                                                 aTAGACCTATCACTGCATAAAATAATGCGATGCCGGGATGGTTTgtatttcccggcatctttatagcgatagcaattatttactgagtgtcgc                                             >  2:679345‑M1/1‑44 (MQ=255)

                                                  tAGACCTATCACTGCATAAAATAATGTGATGCCGGGATGGTTTgtatttcccggcatctttatagcgatagcaattatttactgagtgtcgcg                                            >  2:4519854‑M1/1‑43 (MQ=255)

                                                  tAGACCTATCACTGCATAAAATAATGTGATGCCGGGATGGTTTgtatttcccggcatctttatagcgatagcaattatttactgagtgtcgcg                                            >  2:4904895‑M1/1‑43 (MQ=255)

                                                  tAGACCTATCACTGCATAAAATAATGTGATGCCGGGATGGTTTgtatttcccggcatctttatagcgatagcaattatttactgagtgtcgcg                                            >  1:4992920‑M1/1‑43 (MQ=255)

                                                  tAGACCTATCACTGCATAAAATAATGTGATGCCGGGATGGTTTgtatttcccggcatctttatagcgatagcaattatttactgagtgtcgcg                                            <  2:5944664‑M1/93‑51 (MQ=255)

                                                   aGACCTATCACTGCATAAAATAATGTGATGCCGGGATGGTTTgtatttcccggcatctttatagcgatagcaattatttactgagtgtcgcga                                           <  2:6323414‑M1/93‑52 (MQ=255)

                                                   aGACCTATCACTGCATAAAATAATGTGATGCCGGGATGGTTTgtatttcccggcatctttatagcgatagcaattatttactgagtgtcgcga                                           >  1:5046450‑M1/1‑42 (MQ=255)

                                                    gACCTATCACTGCATAAAATAATGTGATGCCGGGATGGTTTgtatttcccggcatctttatagcgatagcaattatttactgagtgtcgcgac                                          >  2:2803713‑M1/1‑41 (MQ=255)

                                                    gACCTATCACTGCATAAAATAATGTGATGCCGGGATGGTTTgtatttcccggcatctttatagcgatagcaattatttactgagtgtcgcgac                                          >  2:2140579‑M1/1‑41 (MQ=255)

                                                    gACCTATCACTGCATAAAATAATGTGATGCCGGGATGGTTTgtatttcccggcatctttatagcgatagcaattatttactgagtgtcgcgac                                          >  2:1798386‑M1/1‑41 (MQ=255)

                                                     aCCTATCACTGCATAAAATAATGTGATGCCGGGATGGTTTgtatttcccggcatctttatagcgatagcaattatttactgagtgtcgcgacc                                         >  1:4570582‑M1/1‑40 (MQ=255)

                                                      ccTATCACTGCATAAAATAATGTGATGCCGGGATGGTTTgtatttcccggcatctttatagcgatagcaattatttactgagtgtcgcgacca                                        <  2:3304710‑M1/93‑55 (MQ=255)

                                                      ccTATCACTGCATAAAATAATGTGATGCCGGGATGGTTTgtatttcccggcatctttatagcgatagcaattatttactgagtgtcgcgacca                                        <  1:4400145‑M1/93‑55 (MQ=255)

                                                       cTATCACTGCATAAAATAATGTGATGCCGGGATGGTTTgtatttcccggcatctttatagcgatagcaattgtttactgagtgtcgcgaccat                                       >  1:431391‑M1/1‑38 (MQ=255)

                                                       cTATCACTGCATAAAATAATGTGATGCCGGGATGGTTTgtatttcccggcatctttatagcgatagcaattatttactgagtgtcgcgaccat                                       >  2:4812252‑M1/1‑38 (MQ=255)

                                                         aTCACTGCATAAAATAATGTGATGCCGGGATGGTTTgtatttcccggcatctttatagcgatagcaattatttactgagtgtcgcgaccatca                                     >  1:3179406‑M1/1‑36 (MQ=255)

                                                         aTCACTGCATAAAATAATGTGATGCCGGGATGGTTTgtatttcccggcatctttatagcgatagcaattatttactgagtgtcgcgaccatca                                     <  1:5634172‑M1/93‑58 (MQ=255)

                                                          tCACTGCATAAAATAATGTGATGCCGGGATGGTTTgtatttcccggcatctttatagcgatagcaattatttactgagtgtcgcgaccatcac                                    <  1:6490696‑M1/93‑59 (MQ=255)

                                                              tGCATAAAATAATGTGATGCCGGGATGGTTTgtatttcccggcatctttatagcgatagcaattatttactgagtgtcgcgaccatcaccgct                                >  1:921216‑M1/1‑31 (MQ=255)

                                                              tGCATAAAATAATGTGATGCCGGGATGGTTTgtatttcccggcatctttatagcgatagcaattatttactgagtgtcgcgaccatcaccgct                                >  1:2273343‑M1/1‑31 (MQ=255)

                                                              tGCATAAAATAATGTGATGCCGGGATGGTTTgtatttcccggcatctttatagcgatagcaattatttactgagtgtcgcgaccatcaccact                                >  2:3489845‑M1/1‑31 (MQ=255)

                                                                  taaaataatgTGATGCCGGGATGGTTTgtatttcccggcatctttatagcgatagcaattatttactgagtgtcgcgaccatcaccgctttga                            >  2:5379227‑M1/3‑27 (MQ=255)

                                                                  taaaataatgTGATGCCGGGATGGTTTgtatttcccggcatctttatagcgatagcaattatttactgagtgtcgcgaccatcaccgctttga                            >  2:3803557‑M1/3‑27 (MQ=255)

                                                                    aaataaTGTGATGCCGGGATGGTTTgtatttcccggcatctttatagcgatagcaattatttactgagtgtcgcgaccatcaccgctttgata                          <  1:6417403‑M1/93‑69 (MQ=255)

                                                                        aatGTGATGCCGGGATGGTTTgtatttcccggcatctttatagcgatagcaattatttactgagtgtcgcgaccatcaccgctttgatagtat                      <  2:1700048‑M1/93‑73 (MQ=255)

                                                                         atGTGATGCCGGGATGGTTTgtatttcccggcatctttatagcgatagcaattatttactgagtgtcgcgaccatcaccgctttgatagtatg                     >  1:6172545‑M1/1‑20 (MQ=255)

                                                                          tgtgATGCCGGGATGGTTTgtatttcccggcatctttatagcgatagcaattatttactgagtgtcgcgaccatcaccgctttgatagtatgc                    <  1:4635495‑M1/93‑75 (MQ=255)

                                                                            tgATGCCGGGATGGTTTgtatttcccggcatctttatagcgatagcaattatttactgagtgtcgcgaccatcaccgctttgatagtatgcat                  <  1:4376550‑M1/93‑77 (MQ=255)

                                                                             gATGCCGGGATGGTTTgtatttcccggcatctttatagcgatagcaattatttactgagtgtcgcgaccatcaccgctttgatagtatgcata                 <  2:3155095‑M1/93‑78 (MQ=255)

                                                                             gATGCCGGGATGGTTTgtatttcccggcatctttatagcgatagcaattatttactgagtgtcgcgaccatcaccgctttgatagtatgcata                 <  1:1408494‑M1/93‑78 (MQ=255)

                                                                              aTGCCGGGATGGTTTgtatttcccggcatctttatagcgatagcaattatttactgagtgtcgcgaccatcaccgctttgatagtatgcatac                <  1:5486432‑M1/93‑79 (MQ=255)

                                                                              aTGCCGGGATGGTTTgtatttcccggcatctttatagcgatagcaattatttactgagtgtcgcgaccatcaccgctttgatagtatgcatac                <  1:4674671‑M1/93‑79 (MQ=255)

                                                                              aTGCCGGGATGGTTTgtatttcccggcatctttatagcgatagcaattatttactgagtgtcgcgaccatcaccgctttgatagtatgcatac                <  1:1832660‑M1/93‑79 (MQ=255)

                                                                               tGCCGGGATGGTTTgtatttcccggcatctttatagcgatagcaattatttactgagtgttgcgaccatcaccgctttgatagtatgcatacg               >  2:4106138‑M1/1‑14 (MQ=255)

                                                                               tGCCGGGATGGTTTgtatttcccggcatctttatagcgatagcaattatttactgagtgtcgcgaccatcaccgctttgatagtatgcatacg               >  1:4252967‑M1/1‑14 (MQ=255)

                                                                               tGCCGGGATGGTTTgtatttcccggcatctttatagcgatagcaattatttactgagtgtcgcgaccatcaccgctttgatagtatgcatacg               >  1:5916857‑M1/1‑14 (MQ=255)

                                                                                  cGGGATGGTTTgtatttcccggcatctttatagcgatagcaattatttactgagtgtcgcgaccatcaccgctttgatagtatgcatacggtt            <  2:788288‑M1/93‑83 (MQ=255)

                                                                                  cGGGATGGTTTgtatttcccggcatctttatagcgatagcaattatttactgagtgtcgcgaccatcaccgctttgatagtatgcatacggtt            >  2:3413201‑M1/1‑11 (MQ=255)

                                                                                   gggATGGTTTgtatttcccggcatctttatagcgatagcaattatttactgagtgtcgcgaccatcaccgctttgatagtatgcatacggttt           >  2:4598402‑M1/1‑10 (MQ=255)

                                                                                   gggATGGTTTgtatttcccggcatctttatagcgatagcaattatttactgagtgtcgcgaccatcaccgctttgatagtatgcatacggttt           >  1:1025249‑M1/1‑10 (MQ=255)

                                                                                    ggATGGTTTgtatttcccggcatctttatagcgatagcaattatttactgagtgtcgcgaccatcaccgctttgatagtatgcatacggtttt          >  2:6236084‑M1/1‑9 (MQ=255)

                                                                                    ggATGGTTTgtatttcccggcatctttatagcgatagcaattatttactgagtgtcgcgaccatcaccgctttgatagtatgcatacggtttt          >  1:2563719‑M1/1‑9 (MQ=255)

                                                                                    ggATGGTTTgtatttcccggcatctttatagcgatagcaattatttactgagtgtcgcgaccatcaccgctttgatagtatgcatacggtttt          <  1:1364496‑M1/93‑85 (MQ=255)

                                                                                     gATGGTTTgtatttcccggcatctttatagcgatagcaattatttactgagtgtcgcgaccatcaccgctttgatagtatgcatacggttttc         <  2:1354400‑M1/93‑86 (MQ=255)

                                                                                      aTGGTTTgtatttcccggcatctttatagcgatagcaattatttactgagtgtcgcgaccatcaccgctttgatagtatgcatacggttttcc        <  1:2155275‑M1/93‑87 (MQ=255)

                                                                                       tGGTTTgtatttcccggcatctttatagcgatagcaattatttactgagtgtcgcgaccatcaccgctttgatagtatgcatacggttttccg       >  1:1225248‑M1/1‑6 (MQ=255)

                                                                                         gTTTgtatttcccggcatctttatagcgatagcaattatttactgagtgtcgcgaccatcaccgctttgatagtatgcatacggttttccgcc     <  2:5295525‑M1/93‑90 (MQ=255)

                                                                                         gTTTgtatttcccggcatctttatagcgatagcaattatttactgagtgtcgcgaccatcaccgctttgatagtatgcatacggttttccgcc     <  2:3062109‑M1/93‑90 (MQ=255)

                                                                                         gTTTgtatttcccggcatctttatagcgatagcaattatttactgagtgtcgcgaccatcaccgctttgatagtatgcatacggttttccgcc     >  2:6648423‑M1/1‑4 (MQ=255)

                                                                                           ttgtatttcccggcatctttatagcgatagcaattatttactgagtgtcgcgaccatcaccgctttgatagtatgcatacggttttccgcctg   >  2:390050‑M1/1‑2 (MQ=255)

                                                                                            tgtatttcccggcatctttatagcgatagcaattatttactgagtgtcgcgaccatcaccgctttgatagtatgcatacggttttccgcctga  <  2:5074751‑M1/93‑93 (MQ=255)

                                                                                            tgtatttcccggcatctttatagcgatagcaattatttactgagtgtcgcgaccatcaccgctttgatagtatgcatacggttttccgcctga  >  1:4727743‑M1/1‑1 (MQ=255)

                                                                                            tgtatttcccggcatctttatagcgatagcaattatttactgagtgtcgcgaccatcaccgctttgatagtatgcatacggttttccgcctga  <  1:327692‑M1/93‑93 (MQ=255)

AATCCACCGGAAAATTTACGTATAGCAATAGAACACTTTGGGTGGAAGAATAGACCTATCACTGCAT‑AAATAATGTGATGCCGGGATGGTTTCCGCATCTTATAGCGATCAGATTATTTACTGAGCGTCGCGACCATCACCGCTTTGATAGTATGCATACGGTTTTCCGCCTGATCAAAAACAA  >  NC_000913/4474757‑4474940

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`