Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 438,194 A→G V403A (GTG→GCG)  dxs ← 1‑deoxyxylulose‑5‑phosphate synthase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913438,1940AG99.6% 717.4 / ‑4.8 220V403A (GTG→GCG) dxs1‑deoxyxylulose‑5‑phosphate synthase
Reads supporting (aligned to +/- strand):  ref base A (0/1);  new base G (103/116);  total (103/117)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.43e-01

Only 100 of 231 total aligned reads displayed.

CCCTGATGGGTTTGACCGTCAGCACCAACAATGCCCGCGCGGTCGATGGCGAACAGGACCGGAAGCTTTTGAATCGCCACGTCATGCAGCACCTGATCATAGGCGCGTTGCAGGAAAGTGGAGTAAATCGCGACAATGGGTTTGTACCCACCAATCGCCAGACCCGCAGCAAAGGTCACC  >  NC_000913/438104‑438283
                                                                                          |                                                                                         
cccTGATGGGTTTGACCGTCAGCACCAACAATGCCCGCGCGGTCGATGGCGAACAGGACCGGAAGCTTTTGAATCGCCACGTCATGCAGCGcc                                                                                         <  1:1023949/93‑1 (MQ=255)
cccTGATGGGTTTGACCGTCAGCACCAACAATGCCCGCGCGGTCGATGGCGAACAGGACCGGAAGCTTTTGAATCGCCACGTCATGCAGCGcc                                                                                         >  1:5707073/1‑93 (MQ=255)
cccTGATGGGTTTGACCGTCAGCACCAACAATGCCCGCGCGGTCGATGGCGAACAGGACCGGAAGCTTTTGAATCGCCACGTCATGCAGCGcc                                                                                         >  1:1619373/1‑93 (MQ=255)
cccTGATGGGTTTGACCGTCAGCACCAACAATGCCCGCGCGGTCGATGGCGAACAGGACCGGAAGCTTTTGAATCGCCACGTCATGCAGCGcc                                                                                         <  1:3481395/93‑1 (MQ=255)
 ccTGATGGGTTTGACCGTCAGCACCAACAATGCCCGCGCGGTCGATGGCGAACAGGACCGGAAGCTTTTGAATCGCCACGTCATGCAGCGCCt                                                                                        <  1:6089403/93‑1 (MQ=255)
 ccTGATGGGTTTGACCGTCAGCACCAACAATGCCCGCGCGGTCGATGGCGAACAGGACCGGAAGCTTTTGAATCGCCACGTCATGCAGCGCCt                                                                                        >  1:5003966/1‑93 (MQ=255)
  cTGATGTGTTTGACCGTCAGCACCAACAATGCCCGCGCGGTCGATGGCGAACAGGACCGGAAGCTTTTGAATCGCCACGTCATGCAGCGCCTg                                                                                       <  2:2297631/93‑1 (MQ=255)
    gATGGGTTTGACCGTCAGCACCAACAATGCCCGCGCGGTCGATGGCGAACAGGACCGGAAGCTTTTGAATCGCCACGTCATGCAGCGCCTGAt                                                                                     >  1:5327663/1‑93 (MQ=255)
    gATGGGTTTGACCGTCAGCACCAACAATGCCCGCGCGGTCGATGGCGAACAGGACCGGAAGCTTTTGAATCGCCACGTCATGCAGCGCCTGAt                                                                                     >  1:2957215/1‑93 (MQ=255)
    gATGGGTTTGACCGTCAGCACCAACAATGCCCGCGCGGTCGATGGCGAACAGGACCGGAAGCTTTTGAATCGCCACGTCATGCAGCGCCTGAt                                                                                     <  2:4024722/93‑1 (MQ=255)
     aTGGGTTTGACCGTCAGCACCAACAATGCCCGCGCGGTCGATGGCGAACAGGACCGGAAGCTTTTGAATCGCCACGTCATGCAGCGCCTGATc                                                                                    >  1:4441236/1‑93 (MQ=255)
     aTGGGTTTGACCGTCAGCACCAACAATGCCCGCGCGGTCGATGGCGAACAGGACCGGAAGCTTTTGAATCGCCACGTCATGCAGCGCCTGATc                                                                                    <  2:4456209/93‑1 (MQ=255)
      tGGGTTTGACCGTCAGCACCAACAATGCCCGCGCGGTCGATGGCGAACAGGACCGGAAGCTTTTGAATCGCCACGTCATGCAGCGCCTGATCa                                                                                   <  2:2671505/93‑1 (MQ=255)
       gggTTTGACCGTCAGCACCAACAATGCCCGCGCGGTCGATGGCGAACAGGACCGGAAGCTTTTGAATCGCCACGTCATGCAGCGCCTGATCAt                                                                                  <  2:3967190/93‑1 (MQ=255)
        ggTTTGACCGTCAGCACCAACAATGCCCGCGCGGTCGATGGCGAACAGGACCGGAAGCTTTTGAATCGCCACGTCATGCAGCGCCTGATCATa                                                                                 <  1:6511800/93‑1 (MQ=255)
        ggTTTGACCGTCAGCACCAACAATGCCCGCGCGGTCGATGGCGAACAGGACCGGAAGCTTTTGAATCGCCACGTCATGCAGCGCCTGATCATa                                                                                 <  1:2636080/93‑1 (MQ=255)
         gTTTGACCGTCAGCACCAACAATGCCCGCGCGGTCGATGGCGAACAGGACCGGAAGCTTTTGAATCGCCACGTCATGCAGCGCCTGATCATAg                                                                                <  2:819494/93‑1 (MQ=255)
         gTTTGACCGTCAGCACCAACAATGCCCGCGCGGTCGATGGCGAACAGGACCGGAAGCTTTTGAATCGCCACGTCATGCAGCGCCTGATCATAg                                                                                <  1:4113500/93‑1 (MQ=255)
         gTTTGACCGTCAGCACCAACAATGCCCGCGCGGTCGATGGCGAACAGGACCGGAAGCTTTTGAATCGCCACGTCATGCAGCGCCTGATCATAg                                                                                >  1:4170219/1‑93 (MQ=255)
          tttGACCGTCAGCACCAACAATGCCCGCGCGGTCGATGGCGAACAGGACCGGAAGCTTTTGAATCGCCACGTCATGCAGCGCCTGATCATAgg                                                                               >  2:6688725/1‑93 (MQ=255)
          tttGACCGTCAGCACCAACAATGCCCGCGCGGTCGATGGCGAACAGGACCGGAAGCTTTTGAATCGCCACGTCATGCAGCGCCTGATCATAgg                                                                               <  1:4692151/93‑1 (MQ=255)
           ttGACCGTCAGCACCAACAATGCCCGCGCGGTCGATGGCGAACAGGACCGGAAGCTTTTGAATCGCCACGTCATGCAGCGCCTGATCATAGgc                                                                              >  1:22805/1‑93 (MQ=255)
             gACCGTCAGCACCAACAATGCCCGCGCGGTCGATGGCGAACAGGACCGGAAGCTTTTGAATCGCCACGTCATGCAGCGCCTGATCATAGgcgc                                                                            >  2:1302964/1‑93 (MQ=255)
             gACCGTCAGCACCAACAATGCCCGCGCGGTCGATGGCGAACAGGACCGGAAGCTTTTGAATCGCCACGTCATGCAGCGCCTGATCATAGgcgc                                                                            >  2:3106712/1‑93 (MQ=255)
             gACCGTCAGCACCAACAATGCCCGCGCGGTCGATGGCGAACAGGACCGGAAGCTTTTGAATCGCCACGTCATGCAGCGCCTGATCATAGgcgc                                                                            <  2:3112270/93‑1 (MQ=255)
               ccGTCAGCACCAACAATGCCCGCGCGGTCGATGGCGAACAGGACCGGAAGCTTTTGAATCGCCACGTCATGCAGCGCCTGATCATAGGCGCGt                                                                          <  1:3978698/93‑1 (MQ=255)
                cGTCAGCACCAACAATGCCCGCGCGGTCGATGGCGAACAGGACCGGAAGCTTTTGAATCGCCACGTCATGCAGCGCCTGATCATAGGCGCGtt                                                                         >  2:4363731/1‑93 (MQ=255)
                cGTCAGCACCAACAATGCCCGCGCGGTCGATGGCGAACAGGACCGGAAGCTTTTGAATCGCCACGTCATGCAGCGCCTGATCATAGGCGCGtt                                                                         <  2:3413760/93‑1 (MQ=255)
                cGTCAGCACCAACAATGCCCGCGCGGTCGATGGCGAACAGGACCGGAAGCTTTTGAATCGCCACGTCATGCAGCACCTGATCATAGGCGCGtt                                                                         <  1:1226971/93‑1 (MQ=255)
                  tCAGCACCAACAATGCCCGCGCGGTCGATGGCGAACAGGACCGGAAGCTTTTGAATCGCCACGTCATGCAGCGCCTGATCATAGGCGCGTTGc                                                                       >  1:2453411/1‑93 (MQ=255)
                  tCAGCACCAACAATGCCCGCGCGGTCGATGGCGAACAGGACCGGAAGCTTTTGAATCGCCACGTCATACAGCGCCCGATCACAGGCGCGTTGc                                                                       >  1:1976332/1‑93 (MQ=255)
                   cAGCACCAACAATGCCCGCGCGGTCGATGGCGAACAGGACCGGAAGCTTTTGAATCGCCACGTCATGCAGCGCCTGATCATAGGCGCGTTGCa                                                                      >  2:6315234/1‑93 (MQ=255)
                   cAGCACCAACAATGCCCGCGCGGTCGATGGCGAACAGGACCGGAAGCTTTTGAATCGCCACGTCATGCAGCGCCTGATCATAGGCGCGTTGCa                                                                      >  1:5222524/1‑93 (MQ=255)
                   cAGCACCAACAATGCCCGCGCGGTCGATGGCGAACAGGACCGGAAGCTTTTGAATCGCCACGTCATGCAGCGCCTGATCATAGGCGCGTTGCa                                                                      >  1:3196874/1‑93 (MQ=255)
                   cAGCACCAACAATGCCCGCGCGGTCGATGGCGAACAGGACCGGAAGCTTTTGAATCGCCACGTCATGCAGCGCCTGATCATAGGCGCGTTGCa                                                                      <  2:1710138/93‑1 (MQ=255)
                     gCACCAACAATGCCCGCGCGGTCGATGGCGAACAGGACCGGAAGCTTTTGAATCGCCACGTCATGCAGCGCCTGATCATAGGCGCGTTGCAgg                                                                    >  1:3832226/1‑93 (MQ=255)
                      cACCAACAATGCCCGCGCGGTCGATGGCGAACAGGACCGGAAGCTTTTGAATCGCCACGTCATGCAGCGCCTGATCATAGGCGCGTTGCAGGa                                                                   <  2:6725592/93‑1 (MQ=255)
                         caacaaTGCCCGCGCGGTCGATGGCGAACAGGACCGGAAGCTTTTGAATCGCCACGTCATGCAGCGCCTGATCATAGGCGCGTTGCAGGAAAg                                                                <  2:2398827/93‑1 (MQ=255)
                         caacaaTGCCCGCGCGGTCGATGGCGAACAGGACCGGAAGCTTTTGAATCGCCACGTCATGCAGCGCCTGATCATAGGCGCGTTGCAGGAAAg                                                                <  1:86641/93‑1 (MQ=255)
                          aacaaTGCCCGCGCGGTCGATGGCGAACAGGACCGGAAGCTTTTGAATCGCCACGTCATGCAGCGCCTGATCATAGGCGCGTTGCAGGAAAgg                                                               >  2:927260/1‑92 (MQ=255)
                          aacaaTGCCCGCGCGGTCGATGGCGAACAGGACCGGAAGCTTTTGAATCGCCACGTCATGCAGCGCCTGATCATAGGCGCGTTGCAGGAAAGt                                                               >  1:4932923/1‑93 (MQ=255)
                           acaaTGCCCGCGCGGTCGATGGCGAACAGGACCGGAAGCTTTTGAATCGCCACGTCATGCAGCGCCTGATCATAGGCGCGTTGCAGGAAAGTg                                                              >  2:5896012/1‑93 (MQ=255)
                           acaaTGCCCGCGCGGTCGATGGCGAACAGGACCGGAAGCTTTTGAATCGCCACGTCATGCAGCGCCTGATCATAGGCGCGTTGCAGGAAAGTg                                                              <  1:1065251/93‑1 (MQ=255)
                              aTGCCCGCGCGGTCGATGGCGAACAGGACCGGAAGCTTTTGAATCGCCACGTCATGCAGCGCCTGATCATAGGCGCGTTGCAGGAAAGTGGAg                                                           >  2:3359216/1‑93 (MQ=255)
                              aTGCCCGCGCGGTCGATGGCGAACAGGACCGGAAGCTTTTGAATCGCCACGTCATGCAGCGCCTGATCATAGGCGCGTTGCAGGAAAGTGGAg                                                           <  2:2031548/93‑1 (MQ=255)
                                  ccGCGCGGTCGATGGCGAACAGGACCGGAAGCTTTTGAATCGCCACGTCATGCAGCGCCTGATCATAGGCGCGTTGCAGGAAAGTGGAGTaaa                                                       <  1:1154716/93‑1 (MQ=255)
                                  ccGCGCGGTCGATGGCGAACAGGACCGGAAGCTTTTGAATCGCCACGTCATGCAGCGCCTGATCATAGGCGCGTTGCAGGAAAGTGGAGTaaa                                                       <  1:2914996/93‑1 (MQ=255)
                                   cgcgcgGTCGATGGCGAACAGGACCGGAAGCTTTTGAATCGCCACGTCATGCAGCGCCTGATCATAGGCGCGTTGCAGGAAAGTGGAGTAAAt                                                      <  1:1334409/93‑1 (MQ=255)
                                   cgcgcgGTCGATGGCGAACAGGACCGGAAGCTTTTGAATCGCCACGTCATGCAGCGCCTGATCATAGGCGCGTTGCAGGAAAGTGGAGTAAAt                                                      >  2:4423444/1‑93 (MQ=255)
                                       cgGTCGATGGCGAACAGGACCGGAAGCTTTTGAATCGCCACGTCATGCAGCGCCTGATCATAGGCGCGTTGCAGGAAAGTGGAGTAAATcgcg                                                  >  1:2290492/1‑93 (MQ=255)
                                        ggTCGATGGCGAACAGGACCGGAAGCTTTTGAATCGCCACGTCATGCAGCGCCTGATCATAGGCGCGTTGCAGGAAAGTGGAGTAAATCGCGa                                                 <  2:775833/93‑1 (MQ=255)
                                         gTCGATGGCGAACAGGACCGGAAGCTTTTGAATCGCCACGTCATGCAGCGCCTGATCATAGGCGCGTTGCAGGAAAGTGGAGTAAATCGCGAc                                                >  1:5873783/1‑93 (MQ=255)
                                            gATGGCGAACAGGACCGGAAGCTTTTGAATCGCCACGTCATGCAGCGCCTGATCATAGGCGCGTTGCAGGAAAGTGGAGTAAATCGCGACAAt                                             <  1:2229514/93‑1 (MQ=255)
                                             aTGGCGAACAGGACCGGAAGCTTTTGAATCGCCACGTCATGCAGCGCCTGATCATAGGCGCGTTGCAGGAAAGTGGAGTAAATCGCGACAATg                                            <  1:6015562/93‑1 (MQ=255)
                                             aTGGCGAACAGGACCGGAAGCTTTTGAATCGCCACGTCATGCAGCGCCTGATCATAGGCGCGTTGCAGGAAAGTGGAGTAAATCGCGACAATg                                            >  1:1454440/1‑93 (MQ=255)
                                              tGGCGAACAGGACCGGAAGCTTTTGAATCGCCACGTCATGCAGCGCCTGATCATAGGCGCGTTGCAGGAAAGTGGAGTAAATCGCGACAATgg                                           >  2:4285858/1‑93 (MQ=255)
                                              tGGCGAACAGGACCGGAAGCTTTTGAATCGCCACGTCATGCAGCGCCTGATCATAGGCGCGTTGCAGGAAAGTGGAGTAAATCGCGACAATgg                                           <  2:45966/93‑1 (MQ=255)
                                              tGGCGAACAGGACCGGAAGCTTTTGAATCGCCACGTCATGCAGCGCCTGATCATAGGCGCGTTGCAGGAAAGTGGAGTAAATCGCGACAATgg                                           >  2:1985504/1‑93 (MQ=255)
                                               ggCGAACAGGACCGGAAGCTTTTGAATCGCCACGTCATGCAGCGCCTGATCATAGGCGCGTTGCAGGAAAGTGGAGTAAATCGCGACAATggg                                          <  2:1926371/93‑1 (MQ=255)
                                                 cGAACAGGACCGGAAGCTTTTGAATCGCCACGTCATGCAGCGCCTGATCATAGGCGCGTTGCAGGAAAGTGGAGTAAATCGCGACAATGGGtt                                        <  1:4866191/93‑1 (MQ=255)
                                                   aaCAGGACCGGAAGCTTTTGAATCGCCACGTCATGCAGCGCCTGATCATAGGCGCGTTGCAGGAAAGTGGAGTAAATCGCGACAATGGGTTTg                                      >  2:4719756/1‑93 (MQ=255)
                                                    aCAGGACCGGAAGCTTTTGAATCGCCACGTCATGCAGCGCCTGATCATAGGCGCGTTGCAGGAAAGTGGAGTAAATCGCGACAATGGGTTTGt                                     <  2:1260885/93‑1 (MQ=255)
                                                      aGGACCGGAAGCTTTTGAATCGCCACGTCATGCAGCGCCTGATCATAGGCGCGTTGCAGGAAAGTGGAGTAAATCGCGACAATGGGTTTGTAc                                   >  2:5173211/1‑93 (MQ=255)
                                                      aGGACCGGAAGCTTTTGAATCGCCACGTCATGCAGCGCCTGATCATAGGCGCGTTGCAGGAAAGTGGAGTAAATCGCGACAATGGGTTTGTAc                                   >  1:6433355/1‑93 (MQ=255)
                                                      aGGACCGGAAGCTTTTGAATCGCCACGTCATGCAGAGCCTGATCATAGGCGCGTTGCAGGAAAGTGGAGTAAATCGCGACAATGGGATTGTAc                                   >  1:6234295/1‑93 (MQ=255)
                                                       ggACCGGAAGCTTTTGAATCGCCACGTCATGCAGCGCCTGATCATAGGCGCGTTGCAGGAAAGTGGAGTAAATCGCGACCATGGGTTTGTAcc                                  >  1:4349260/1‑93 (MQ=255)
                                                       ggACCGGAAGCTTTTGAATCGCCACGTCATGCAGCGCCTGATCATAGGCGCGTTGCAGGAAAGTGGAGTAAATCGCGACAATGGGTTTGTAcc                                  >  2:1354732/1‑93 (MQ=255)
                                                         aCCGGAAGCTTTTGAATCGCCACGTCATGCAGCGCCTGATCATAGGCGCGTTGCAGGAAAGTGGAGTAAATCGCGACAATGGGTTTGTACcca                                <  2:2967327/93‑1 (MQ=255)
                                                          ccGGAAGCTTTTGAATCGCCGCGTCATGCAGCGCCTGATCATAGGCGCGTTGCAGGAAAGTGGAGTAAATCGCGACAATGGGTTTGTACccac                               >  1:5444417/1‑93 (MQ=255)
                                                          ccGGAAGCTTTTGAATCGCCACGTCATGCAGCGCCTGATCATAGGCGCGTTGCAGGAAAGTGGAGTAAATCGCGACAATGGGTTTGTACccac                               >  2:1626541/1‑93 (MQ=255)
                                                            ggAAGCTTTTGAATCGCCACGTCATGCAGCGCCTGATCATAGGCGCGTTGCAGGAAAGTGGAGTAAATCGCGACAATGGGTTTGTACccacca                             >  2:2556018/1‑93 (MQ=255)
                                                             gAAGCTTTTGAATCGCCACGTCATGCAGCGCCTGATCATAGGCGCGTTGCAGGAAAGTGGAGTAAATCGCGACAATGGGTTTGTACCCACCaa                            <  1:4645515/93‑1 (MQ=255)
                                                             gAAGCTTTTGAATCGCCACGTCATGCAGCGCCTGATCATAGGCGCGTTGCAGGAAAGTGGAGTAAATCGCGACAATGGGTTTGTACCCACCaa                            <  1:6079680/93‑1 (MQ=255)
                                                              aaGCTTTTGAATCGCCACGTCATGCAGCGCCTGATCATAGGCGCGTTGCAGGAAAGTGGAGTAAATCGCGACAATGGGTTTGTACCCACCAAt                           <  2:3022316/93‑1 (MQ=255)
                                                              aaGCTTTTGAATCGCCACGTCATGCAGCGCCTGATCATAGGCGCGTTGCAGGAAAGTGGAGTAAATCGCGACAATGGGTTTGTACCCACCAAt                           >  1:376488/1‑93 (MQ=255)
                                                               aGCTTTTGAATCGCCACGTCATGCAGCGCCTGATCATAGGCGCGTTGCAGGAAAGTGGAGTAAATCGCGACAATGGGTTTGTACTCACCAATc                          >  1:6726978/1‑93 (MQ=255)
                                                                gCTTTTGAATCGCCACGTCATGCAGCGCCTGATCATAGGCGCGTTGCAGGAAAGTGGAGTAAATCGCGACAATGGGTTTGTACCCACCAATCg                         <  2:5593237/93‑1 (MQ=255)
                                                                   tttGAATCGCCACGTCATGCAGCGCCTGATCATAGGCGCGTTGCAGGAAAGTGGAGTAAATCGCGACAATGGGTTTGTACCCACCAATCGCCa                      >  2:3927167/1‑93 (MQ=255)
                                                                    ttGAATCGCCACGTCATGCAGCGCCTGATCATAGGCGCGTTGCAGGAAAGTGGAGTAAATCGCGACAATGGGTTTGTACCCACCAATCGCCAg                     <  2:5192785/93‑1 (MQ=255)
                                                                       aaTCGCCACGTCATGCAGCGCCTGATCATAGGCGCGTTGCAGGAAAGTGGAGTAAATCGCGACAATGGGTTTGTACCCACCAATCGCCAGAcc                  <  2:37482/93‑1 (MQ=255)
                                                                       aaTCGCCACGTCATGCAGCGCCTGATCATAGGCGCGTTGCAGGAAAGTGGAGTAAATCGCGACAATGGGTTTGTACCCACCAATCGCCAGAcc                  <  1:6144659/93‑1 (MQ=255)
                                                                        aTCGCCACGTCATGCAGCGCCTGATCATAGGCGCGTTGCAGGAAAGTGGAGTAAATCGCGACAATGGGTTTGTACCCACCAATCGCCAGAccc                 <  2:702628/93‑1 (MQ=255)
                                                                        aTCGCCACGTCATGCAGCGCCTGATCATAGGCGCGTTGCAGGAAAGTGGAGTAAATCGCGACAATGGGTTTGTACCCACCAATCGCCAGAccc                 >  2:5494048/1‑93 (MQ=255)
                                                                         tCGCCACGTCATGCAGCGCCTGATCATAGGCGCGTTGCAGGAAAGTGGAGTAAATCGCGACAATGGGTTTGTACCCACCAATCGCCAGACCCg                >  1:6686450/1‑93 (MQ=255)
                                                                           gCCACGTCATGCAGCGCCTGATCATAGGCGCGTTGCAGGAAAGTGGAGTAAATCGCGACAATGGGTTTGTACCCACCAATCGCCAGACCCgca              >  2:6331961/1‑93 (MQ=255)
                                                                           gCCACGTCATGCAGCGCCTGATCATAGGCGCGTTGCAGGAAAGTGGAGTAAATCGCGACAATGGGTTTGTACCCACCAATCGCCAGACCCgca              >  2:6193776/1‑93 (MQ=255)
                                                                            ccACGTCATGCAGCGCCTGATCATAGGCGCGTTGCAGGAAAGTGGAGTAAATCGCGACCATGGGTTTGTACCCACCAATCGCAAGACCCgcag             >  1:5755162/1‑93 (MQ=255)
                                                                            ccACGTCATGCAGCGCCTGATCATAGGCGCGTTGCAGGAAAGTGGAGTAAATCGCGACAATGGGTTTGTACCCACCAATCGCCAGACCCgcag             <  2:6346039/93‑1 (MQ=255)
                                                                             cACGTCATGCAGCGCCTGATCATAGGCGCGTTGCAGGAAAGTGGAGTAAATCGCGACAATGGGTTTGTACCCACCAATCGCCAGACCCgcagc            <  2:4962653/93‑1 (MQ=255)
                                                                             cACGTCATGCAGCGCCTGATCATAGGCGCGTTGCAGGAAAGTGGAGGAAATCGCGACAATGGGTTTGTACCCACCAATCGCCAGACCCgcagc            >  1:1540352/1‑93 (MQ=255)
                                                                              aCGTCATGCAGCGCCTGATCATAGGCGCGTTGCAGGAAAGTGGAGTAAATCGCGACAATGGGTTTGTACCCACCAATCGCCAGACCCgcagca           >  1:2770894/1‑93 (MQ=255)
                                                                               cGTCATGCAGCGCCTGATCATAGGCGCGTTGCAGGAAAGTGGAGTAAATCGCGACAATGGGTTTGTACCCACCAATCGCCAGACCCGCAGCaa          <  2:293455/93‑1 (MQ=255)
                                                                               cGTCATGCAGCGCCTGATCATAGGCGCGTTGCAGGAAAGTGGAGTAAATCGCGACAATGGGTTTGTACCCACCAATCGCCAGACCCGCAGCaa          >  2:3693755/1‑93 (MQ=255)
                                                                                gTCATGCAGCGCCTGATCATAGGCGCGTTGCAGGAAAGTGGAGTAAATCGCGACAATGGGTTTGTACCCACCAATCGCCAGACCCGCAGCaaa         <  2:5818082/93‑1 (MQ=255)
                                                                                gTCATGCAGCGCCTGATCATAGGCGCGTTGCAGGAAAGTGGAGTAAATCGCGACAATGGGTTTGTACCCACCAATCGCCAGACCCGCAGCaaa         >  1:982004/1‑93 (MQ=255)
                                                                                 tCATGCAGCGCCTGATCATAGGCGCGTTGCAGGAAAGTGGAGTAAATCGCGACAATGGGTTTGTACCCACCAATCGCCAGACCCGCAGCAAAg        <  2:5233476/93‑1 (MQ=255)
                                                                                   aTGCAGCGCCTGATCATAGGCGCGTTGCAGGAAAGTGGAGTAAATCGCGACAATGGGTTTGTACCCACCAATCGCCAGACCCGCAGCAAAGGt      >  1:2564731/1‑93 (MQ=255)
                                                                                    tGCAGCGCCTGATCATAGGCGCGTTGCAGGAAAGTGGAGTAAATCGCGACAATGGGTTTGTACCCACCAATCGCCAGACCCGCAGCAAAGGTc     >  2:588042/1‑93 (MQ=255)
                                                                                     gcagcgCCTGATCATAGGCGCGTTGCAGGAAAGTGGAGTAAATCGCGACAATGGGTTTGTACCCACCAATCGCCAGACCCGCAGCAAAGGTCa    >  1:1893031/1‑93 (MQ=255)
                                                                                       agcgCCTGATCATAGGCGCGTTGCAGGAAAGTGGAGTAAATCGCGACAATGGGTTTGTACCCACCAATCGCCAGACCCGCAGCAAAGGTCAcc  <  2:5791640/93‑1 (MQ=255)
                                                                                          |                                                                                         
CCCTGATGGGTTTGACCGTCAGCACCAACAATGCCCGCGCGGTCGATGGCGAACAGGACCGGAAGCTTTTGAATCGCCACGTCATGCAGCACCTGATCATAGGCGCGTTGCAGGAAAGTGGAGTAAATCGCGACAATGGGTTTGTACCCACCAATCGCCAGACCCGCAGCAAAGGTCACC  >  NC_000913/438104‑438283

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑