BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,586,561	T→C	N97S (AAC→AGC)	ugpC ←	ATP‑binding component of sn‑glycerol 3‑phosphate transport system

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,586,561	0	T	C	100.0%	1012.8 / NA	312	N97S (AAC→AGC)	ugpC	ATP‑binding component of sn‑glycerol 3‑phosphate transport system
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (144/168); total (144/168)

Only 100 of 324 total aligned reads displayed.

CGCTCGGCAATTTGCTGCTTGCCCATGCCGCGAATTTTCAGCCCCCACGCCATGTTTTCTTCGACACTCATATGCGGATAAAGCGCGTAGTTCTGGAACACCATCGCAATCCCGCGATCTTTTGGCTCCATTTCAGTCACGCGCTGGTCGTTGATCCAGATATCGCCTTCTGTCACCCG  >  NC_000913/3586471‑3586649

cgcTCGGCAATTTGCTGCTTGCCCATGCCGCGAATTTTCAGCCCCCACGCCATGTTTTCTTCGACACTCATATGCGGATAAAGCGCGTAGCTc                                                                                        <  2:6267527/93‑1 (MQ=255)

 gcTCGGCAATTTGCTGCTTGCCCATGCCGCGAATTTTCATCCCCCACGCCATGTTTTCTTCGACACTCATATGCGGATAAAGCGCGTAGctct                                                                                       <  2:4279240/93‑1 (MQ=255)

 gcTCGGCAATTTGCTGCTTGCCCATGCCGCGAATTTTCAGCCCCCACGCCATGTTTTCTTCGACACTCATATGCGGATAAAGCGCGTAGctct                                                                                       >  1:6651881/1‑93 (MQ=255)

  cTCGGCAATTTGCTGCTTGCCCATGCCGCGAATTTTCAGCCCCCACGCCATGTTTTCTTCGACACTCATATGCGGATAAAGCGCGTAGCTCTg                                                                                      >  2:2612160/1‑93 (MQ=255)

  cTCGGCAATTTGCTGCTTGCCCATGCCGCGAATTTTCAGCCCCCACGCCATGTTTTCTTCGACACTCATATGCGGATAAAGCGCGTAGCTCTg                                                                                      >  1:2346103/1‑93 (MQ=255)

  cTCGGCAATTTGCTGCTTGCCCATGCCGCGAATTTTCAGCCCCCACGCCATGTTTTCTTCGACACTCACATGCGGATAAAGCGCGTAGCTCTg                                                                                      <  2:1244403/93‑1 (MQ=255)

   tCGGCAATTTGCTGCTTGCCCATGCCGCGAATTTTCAGCCCCCACGCCATGTTTTCTTCGACACTCATATGCGGATAAAGCGCGTAGCTCTgg                                                                                     <  1:837470/93‑1 (MQ=255)

    cGGCAATTTGCTGCTTGCCCATGCCGCGAATTTTCAGCCCCCCCGCCATGTTTTCTTCGACACTCATATGCGGATAAAGCGCGTAGCTCTGGa                                                                                    >  1:3809227/1‑93 (MQ=255)

    cGGCAATTTGCTGCTTGCCCATGCCGCGAATTTTCAGCCCCCACGCCATGTTTTCTTCGACACTCATATGCGGATAAAGCGCGTAGCTCTGGa                                                                                    <  2:1441997/93‑1 (MQ=255)

     ggCAATTTGCTGCTTGCCCATGCCGCGAATTTTCAGCCCCCACGCCATGTTTTCTTCGACACTCATATGCGGATAAAGCGCGTAGCTCTGGaa                                                                                   <  2:2157508/93‑1 (MQ=255)

      gCAATTTGCTGCTTGCCCATGCCGCGAATTTTCAGCCCCCACGCCATGTTTTCTTCGACACTCATATGCGGATAAAGCGCGTAGCTCTGGAac                                                                                  >  2:2984837/1‑93 (MQ=255)

      gCAATTTGCTGCTTGCCCATGCCGCGAATTTTCAGCCCCCACGCCATGTTTTCTTCGACACTCATATGCGGATAAAGCGCGTAGCTCTGGAac                                                                                  <  1:5579922/93‑1 (MQ=255)

      gCAATTTGCTGCTTGCCCATGCCGCGAATTTTCAACCCCCACGCCATGTTTTCTTCGACACTCATATGCGGATAAAGCGCGTAGCTCTGGAac                                                                                  >  1:1003260/1‑93 (MQ=255)

        aaTTTGCTGCTTGCCCATGCCGCGAATTTTCAGCCCCCACGCCATGTTTTCTTCGACACTCATATGCGGATAAAGCGCGTAGCTCTGGAacac                                                                                <  1:6273319/93‑1 (MQ=255)

        aaTTTGCTGCTTGCCCATGCCGCGAATTTTCAGCCCCCACGCCATGTTTTCTTCGACACTCATATGCGGATAAAGCGCGTAGCTCTGGAacac                                                                                >  2:4771967/1‑93 (MQ=255)

         aTTTGCTGCTTGCCCATGCCGCGAATTTTCAGCCCCCACGCCATGTTTTCTTCGACACTCATATGCGGATAAAGCGCGTAGCTCTGGAACAcc                                                                               >  1:4979729/1‑93 (MQ=255)

          tttGCTGCTTGCCCATGCCGCGAATTTTCAGCCCCCACGCCATGTTTTCTTCGACACTCATATGCGGATAAAGCGCGTAGCTCTGGAACACCa                                                                              <  1:1389557/93‑1 (MQ=255)

          tttGCTGCTTGCCCATGCCGCGAATTTTCAGCCCCCACGCCATGTTTTCTTCGACACTCATATGCGGATAAAGCGCGTAGCTCTGGAACACCa                                                                              >  1:205877/1‑93 (MQ=255)

            tgctgcTTGCCCATGCCGCGAATTTTCAGCCCCCACGCCATGTTTTCTTCGACACTCATATGCGGATAAAGCGCGTAGCTCTGGAACACCATc                                                                            >  2:5481/1‑93 (MQ=255)

                  ttGCCCATGCCGCGAATTTTCAGCCCCCACGCCATGTTTTCTTCGACACTCATATGCGGATAAAGCGCGTAGCTCTGGAACACCATCGCAATc                                                                      <  1:695087/93‑1 (MQ=255)

                   tGCCCATGCCGCGAATTTTCAGCCCCCACGCCATGTTTTCTTCGACACTCATATGCGGATAAAGCGCGTAGCTCTGGAACACCATCGCAATcc                                                                     >  1:6385650/1‑93 (MQ=255)

                      ccATGCCGCGAATTTTCAGCCCCCACGCCATGTTTTCTTCGACACTCATATGCGGATAAAGCGCGTAGCTCTGGAACACCATCGCAATCCcgc                                                                  >  2:3237008/1‑93 (MQ=255)

                       cATGCCGCGAATTTTCAGCCCCCACGCCATGTTTTCTTCGACACTCATATGCGGATAAAGCGCGTAGCTCTGGAACACCATCGCAATCCcgcg                                                                 >  2:5920043/1‑93 (MQ=255)

                         tGCCGCGAATTTTCAGCCCCCACGCCATGTTTTCTTCGACACTCATATGCGGATAAAGCGCGTAGCTCTGGAACACCATCGCAATCCCGCGAt                                                               <  2:4489593/93‑1 (MQ=255)

                          gCCGCGAATTTTCAGCCCCCACGCCATGTTTTCTTCGACACTCATATGCGGATAAAGCGCGTAGCTCTGGAACACCATCGCAATCCCGCGATc                                                              >  1:3143500/1‑93 (MQ=255)

                           ccGCGAATTTTCAGCCCCCACGCCATGTTTTCTTCGACACTCATATGCGGATAAAGCGCGTAGCTCTGGAACACCATCGCAATCCCGCGATCt                                                             <  1:2178923/93‑1 (MQ=255)

                               gAATTTTCAGCCCCCACGCCATGTTTTCTTCGACACTCATATGCGGATAAAGCGCGTAGCTCTGGAACACCATCGCAATCCCGCGATCTTTTg                                                         >  2:5730762/1‑93 (MQ=255)

                                aaTTTTCAGCCCCCACGCCATGTTTTCTTCGACACTCCTATGCGGATAAAGCGCGTAGCTCTGGAACACCATCGCAATCCCGCGATCTTTTgc                                                        >  2:1308148/1‑92 (MQ=255)

                                aaTTTTCAGCCCCCACGCCATGTTTTCTTCGACACTCATATGCGGATAAAGCGCGTAGCTCTGGAACACCATCGCAATCCCGCGATCTTTTgg                                                        <  1:3379753/93‑1 (MQ=255)

                                 aTTTTCAGCCCCCACGCCATGTTTTCTTCGACACTCATATGCGGATAAAGCGCGTAGCTCTGGAACACCATCGCAATCCCGCGATCTTTTGGc                                                       <  2:1683912/93‑1 (MQ=255)

                                  ttttCAGCCCCCACGCCATGTTTTCTTCGACACTCATATGCGGATAAAGCGCGTAGCTCTGGAACACCATCGCAATCCCGCGATCTTTTGGCt                                                      >  1:1932865/1‑93 (MQ=255)

                                   tttCAGCCCCCACGCCATGTTTTCTTCGACACTCATATGCGGATAAAGCGCGTAGCTCTGGAACACCATCGCAATCCCGCGATCTTTTGGCTc                                                     <  2:458323/93‑1 (MQ=255)

                                    ttCAGCCCCCACGCCATGTTTTCTTCGACACTCATATGCGGATAAAGCGCGTAGCTCTGGAACACCATCGCAATCCCGCGCTCTTTTGGCTcc                                                    >  1:519537/1‑93 (MQ=255)

                                    ttCAGCCCCCACGCCATGTTTTCTTCGACACTCATATGCGGATAAAGCGCGTAGCTCTGGAACACCATCGCAATCCCGCGATCTTTTGGCTcc                                                    <  1:1243367/93‑1 (MQ=255)

                                    ttCAGCCCCCACGCCATGTTTTCTTCGACACTCATATGCGGATAAAGCGCGTAGCTCTGGAACACCATCGCAATCCCGCGATCTTTTGGCTcc                                                    >  1:2033057/1‑93 (MQ=255)

                                     tCAGCCCCCACGCCATGTTTTCTTCGACACTCATATGCGGATAAAGCGCGTAGCTCTGGAACACCATCGCAATCCCGCGATCTTTTGGCTCCa                                                   >  2:660930/1‑93 (MQ=255)

                                       aGCCCCCACGCCATGTTTTCTTCGACACTCATATGCGGATAAAGCGCGTAGCTCTGGAACACCATCGCAATCCCGCGATCTTTTGGCTCCAtt                                                 <  1:1799125/93‑1 (MQ=255)

                                        gCCCCCACGCCATGTTTTCTTCGACACTCATATGCGGATAAAGCGCGTAGCTCTGGAACACCATCGCAATCCCGCGATCTTTTGGCTCCAttt                                                >  1:6132139/1‑93 (MQ=255)

                                          ccccACGCCATGTTTTCTTCGACACTCATATGCGGATAAAGCGCGTAGCTCTGGAACACCATCGCAATCCCGCGATCTTTTGGCTCCATTTCa                                              <  2:5805601/93‑1 (MQ=255)

                                          ccccACGCCATGTTTTCTTCGACACTCATATGCGGATAAAGCGCGTAGCTCTGGAACACCATCGCAATCCCGCGATCTTTTGGCTCCATTTCa                                              <  1:170791/93‑1 (MQ=255)

                                           ctcACGCCATGTTTTCTTCGACACTCATATGCGGATAAAGCGCGTAGCTCTGGAACACCATCGCAATGCCGCGATCTTTTGGCTCCATTTCAg                                             >  1:4720802/3‑93 (MQ=255)

                                           cccACGCCATGTTTTCTTCGACACTCATATGCGGATAAAGCGCGTAGCTCTGGAACACCATCGCAATCCCGCGATCTTTTGGCTCCATTTCAg                                             <  2:556166/93‑1 (MQ=255)

                                           cccACGCCATGTTTTCTTCGACACTCATATGCGGATAAAGCGCGTAGCTCTGGAACACCATCGCAATCCCGCGATCTTTTGGCTCCATTTCAg                                             >  1:325097/1‑93 (MQ=255)

                                           cccACGCCATGTTTTCTTCGACACTCATATGCGGATAAAGCGCGTAGCTCTGGAACACCATCGCAATCCCGCGATCTTTTGGCTCCATTTCAg                                             >  1:1684402/1‑93 (MQ=255)

                                           cccACGCCATGTTTTCTTCGACACTCATATGCGGATAAAGCGCGTAGCTCTGGAACACCATCGCAATCCCGCGATCTTTTGGCTCCATTTCAg                                             >  2:6122795/1‑93 (MQ=255)

                                            ccACGCCATGTTTTCTTCGACACTCATATGCGGATAAAGCGCGTAGCTCTGGAACACCATCGCAATCCCGCGATCTTTTGGCTCCATTTCAGt                                            >  2:868404/1‑93 (MQ=255)

                                            ccACGCCATGTTTTCTTCGACACTCATATGCGGATAAAGCGCGTAGCTCTGGAACACCATCGCAATCCCGCGATCTTTTGGCTCCATTTCAGt                                            >  1:2408809/1‑93 (MQ=255)

                                             cACGCCATGTTTTCTTCGACACTCATATGCGGATAAAGCGCGTAGCTCTGGAACACCATCGCAATCCCGCGATCTTTTGGCTCCATTTCAGTc                                           <  2:1928584/93‑1 (MQ=255)

                                              aCGCCATGTTTTCTTCGACACTCATATGCGGATAAAGCGCGTAGCTCTGGAACACTATCGCAATCCCGCGATCTTTTGGCTCCATTTCAGTCa                                          <  1:6515785/93‑1 (MQ=255)

                                              aCGCCATGTTTTCTTCGACACTCATATGCGGATAAAGCGCGTAGCTCTGGAACACCATCGCAATCCCGCGATCTTTTGGCTCCATTTCAGTCa                                          <  1:4110069/93‑1 (MQ=255)

                                               cGCCATGTTTTCTTCGACACTCATATGCGGATAAAGCGCGTAGCTCTGGAACACCATCGCAATCCCGCGATCTTTTGGCTCCATTTCAGTCAc                                         <  1:907563/93‑1 (MQ=255)

                                               cGCCATGTTTTCTTCGACACTCATATGCGGATAAAGCGCGTAGCTCTGGAACACCATCGCAATCCCGCGATCTTTTGGCTCCATTTCAGTCAc                                         <  2:2849101/93‑1 (MQ=255)

                                                gCCATGTTTTCTTCGACACTCATATGCGGATAAAGCGCGTAGCTCTGGAACACCATCGCAATCCCGCGATCTTTTGGCTCCATTTCAGTCAcg                                        <  2:3083383/93‑1 (MQ=255)

                                                 ccATGTTTTCTTCGACACTCATATGCGGATAAAGCGCGTAGCTCTGGAACACCATCGCAATCCCGCGATCTTTTGGCTCCATTTCAGTCAcgc                                       >  2:4068664/1‑93 (MQ=255)

                                                  cATGTTTTCTTCGACACTCATATGCGGATAAAGCGCGTAGCTCTGGAACACCATCGCAATCCCGCGATCTTTTGGCTCCATTTCAGTCAcgcg                                      >  2:666644/1‑93 (MQ=255)

                                                  cATGTTTTCTTCGACACTCATATGCGGATAAAGCGCGTAGCTCTGGAACACCATCGCAATCCCGCGATCTTTTGGCTCCATTTCAGTCAcgcg                                      >  2:1743913/1‑93 (MQ=255)

                                                   aTGTTTTCTTCGACACTCATATGCGGATAAAGCGCGTAGCTCTGGAACACCATCGCAATCCCGCGATCTTTTGGCTCCATTTCAGTCACgcgc                                     >  2:1850397/1‑93 (MQ=255)

                                                    tGTTTTCTTCGACACTCATATGCGGATAAAGCGCGTAGCTCTGGAACACCATCGCAATCCCGCGATCTTTTGGCTCCATTTCAGTCACGCGCt                                    >  2:6013698/1‑93 (MQ=255)

                                                    tGTTTTCTTCGACACTCATATGCGGATAAAGCGCGTAGCTCTGGAACACCATCGCAATCCCGCGATCTTTTGGCTCCATTTCAGTCACGCGCt                                    <  1:4664339/93‑1 (MQ=255)

                                                    tGTTTTCTTCGACACTCATATGCGGATAAAGCGCGTAGCTCTGGAACACAATCGCAATCCCGCGATCTTTTGGCTCCATTTCAGTCACGCGCt                                    >  2:1626659/1‑93 (MQ=255)

                                                     gTTTTCTTCGACACTCATATGCGGATAAAGCGCGTAGCTCTGGAACACCATCGCAATCCCGCGATCTTTTGGCTCCATTTCAGTCACGCGCTg                                   >  2:2486941/1‑93 (MQ=255)

                                                      ttttCTTCGACACTCATATGCGGATAAAGCGCGTAGCTCTGGAACACCATCGCAATCCCGCGATCTTTTGGCTCCATTTCAGTCACGCGCTgg                                  >  2:3360394/1‑93 (MQ=255)

                                                       tttCTTCGACACTCATATGCGGATAAAGCGCGTAGCTCTGGAACACCATCGCAATCCCGCGATCTTTTGGCTCCATTTCAGTCACGCGCTGGt                                 <  2:2691946/93‑1 (MQ=255)

                                                           ttcGACACTCATATGCGGATAAAGCGCGTAGCTCTGGAACACCATCGCAATCCCGCGATCTTTTGGCTCCATTTCAGTCACGCGCTGGTCGtt                             >  1:4838886/1‑93 (MQ=255)

                                                           ttcGACACTCATATGCGGATAAAGCGCGTAGCTCTGGAACACCATCGCAATCCCGCGATCTTTTGGCTCCATTTCAGTCACGCGCTGGTCGtt                             <  1:3989975/93‑1 (MQ=255)

                                                            tcGACACTCATATGCGGATAAAGCGCGTAGCTCTGGAACACCATCGCAATCCCGCGATCTTTTGGCTCCATTTCAGTCACGCGCTGGTCGTTg                            <  2:2914028/93‑1 (MQ=255)

                                                             cGACACTCATATGCGGATAAAGCGCGTAGCTCTGGAACACCATCGCAATCCCGCGATCTTTTGGCTCCATTTCAGTCACGCGCTGGTCGTTGa                           <  1:5079936/93‑1 (MQ=255)

                                                             cGACACTCATATGCGGATAAAGCGCGTAGCTCTGGAACACCATCGCAATCCCGCGATCTTTTGGCTCCATTTCAGTCACGCGCTGGTCGTTGa                           <  1:2387949/93‑1 (MQ=255)

                                                              gACACTCATATGCGGATAAAGCGCGTAGCTCTGGAACACCATCGCAATCCCGCGATCTTTTGGCTCCATTTCAGTCACGCGCTGGTCGTTGAt                          <  1:4274784/93‑1 (MQ=255)

                                                                cacTCATATGCGGATAAAGCGCGTAGCTCTGGAACACCATCGCAATCCCGCGATCTTTTGGCTCCATTTCAGTCACGCGCTGGTCGTTGATcc                        <  1:1053380/93‑1 (MQ=255)

                                                                cacTCATATGCGGATAAAGCGCGTAGCTCTGGAACACCATCGCAATCCCGCGATCTTTTGGCTCCATTTCAGTCACGCGCTGGTCGTTGATcc                        >  2:3946487/1‑93 (MQ=255)

                                                                cacTCATATGCGGATAAAGCGCGTAGCTCTGGAACACCATCGCAATCCCGCGATCTTTTGGCTCCATTTCAGTCACGCGCTGGTCGTTGATcc                        >  2:3058839/1‑93 (MQ=255)

                                                                 acTCATATGCGGATAAAGCGCGTAGCTCTGGAACACCATCGCAATCCCGCGATCTTTTGGCTCCATTTCAGTCACGCGCTGGTCGTTGATCCa                       >  2:2289186/1‑93 (MQ=255)

                                                                 acTCATATGCGGATAAAGCGCGTAGCTCTGGAACACCATCGCAATCCCGCGATCTTTTGGCTCCATTTCAGTCACGCGCTGGTCGTTGATCCa                       >  1:4131710/1‑93 (MQ=255)

                                                                   tCATATGCGGATAAAGCGCGTAGCTCTGGAACACCATCGCAATCCCGCGATCTTTTGGCTCCATTTCAGTCACGCGCTGGTCGTTGATCCAGa                     >  2:2399003/1‑93 (MQ=255)

                                                                   tCATATGCGGATAAAGCGCGTAGCTCTGGAACACCATCGCAATCCCGCGATCTTTTGGCTCCATTTCAGTCACGCGCTGGTCGTTGATCCAGa                     <  2:5136568/93‑1 (MQ=255)

                                                                    cATATGCGGATAAAGCGCGTAGCTCTGGAACACCATCGCAATCCCGCGATCTTTTGGCTCCATTTCAGTCACGCGCTGGTCGTTGATCCAGat                    <  1:1493365/93‑1 (MQ=255)

                                                                    cATATGCGGATAAAGCGCGTAGCTCTGGAACACCATCGCAATCCCGCGATCTTTTGGCTCCATTTCAGTCACGCGCTGGTCGTTGATCCAGat                    <  2:4888463/93‑1 (MQ=255)

                                                                     atatGCGGATAAAGCGCGTATCTCTGGAACGCCATCGCAATCCCGCGATCTTTTGGCTCAATTTCAGTCACGCGCTGGTCGTTGATCCAGata                   <  1:4377573/93‑1 (MQ=255)

                                                                     atatGCGGATAAAGCGCGTAGCTCTGGAACACCATCGCAATCCCGCGATCTTTTGGCTCCATTTCAGTCACGCGCTGGTCGTTGATCCAGata                   >  1:1586333/1‑93 (MQ=255)

                                                                     atatGCGGATAAAGCGCGTAGCTCTGGAACACCATCGCAATCCCGCGATCTTTTGGCTCCATTTCAGTCACGCGCTGGTCGTTGATCCAGata                   <  1:3308770/93‑1 (MQ=255)

                                                                       atGCGGATAAAGCGCGTAGCTCTGGAACACCATCGCAATCCCGCGATCTTTTGGCTCCATTTCAGTCACGCGCTGGTCGTTGATCCAGATATc                 <  2:1087196/93‑1 (MQ=255)

                                                                       atGCGGATAAAGCGCGTAGCTCTGGAACACCATCGCAATCCCGCGATCTTTTGGCTCCATTTCAGTCACGCGCTGGTCGTTGATCCAGATATc                 <  2:3682260/93‑1 (MQ=255)

                                                                       atGCGGATAAAGCGCGTAGCTCTGGAACACCATCGCAATCCCGCGATCTTTTGGCTCCATTTCAGTCACGCGCTGGTCGTTGATCCAGATATc                 <  2:4195571/93‑1 (MQ=255)

                                                                          cGGATAAAGCGCGTAGCTCTGGAACACCATCGCAATCCCGCGATCTTTTGGCTCCATTTCAGTCACGCGCTGGTCGTTGATCCAGATATCGcc              >  2:5343686/1‑93 (MQ=255)

                                                                           ggATAAAGCGCGTAGCTCTGGAACACCATCGCAATCCCGCGATCTTTTGGCTCCATTTCAGTCACGCGCTGGTCGTTGATCCAGATATCGCCt             <  2:4946968/93‑1 (MQ=255)

                                                                            gATAAAGCGCGTAGCTCTGGAACACCATCGCAATCCCGCGATCTTTTGGCTCCATTTCAGTCACGCGCTGGTCGTTGATCCAGATATCGCCtt            >  1:5921151/1‑93 (MQ=255)

                                                                            gATAAAGCGCGTAGCTCTGGAACACCATCGCAATCCCGCGATCTTTTGGCTCCATTTCAGTCACGCGCTGGTCGTTGATCCAGATATCGCCtt            >  1:2290800/1‑93 (MQ=255)

                                                                             aTAAAGCGCGTAGCTCTGGAACACCATCGCAATCCCGCGATCTTTTGGCTCCATTTCAGTCACGCGCTGGTCGTTGATCCAGATATCGCCtcc           <  2:6481249/93‑3 (MQ=255)

                                                                             aTAAAGCGCGTAGCTCTGGAACACCATCGCAATCCCGCGATCTTTTGGCTCCATTTCAGTCACGCGCTGGTCGTTGATCCAGATATCGCCTTc           <  1:6066765/93‑1 (MQ=255)

                                                                              tAAAGCGCGTAGCTCTGGAACACCATCGCAATCCCGCGATCTTTTGGCTCCATTTCAGTCACGCGCTGGTCGTTGATCCAGATATCGCCTTCt          >  1:2781746/1‑93 (MQ=255)

                                                                               aaaGCGCGTAGCTCTGGAACACCATCGCAATCCCGCGATCTTTTGGCTCCATTTCAGTCACGCGCTGGTCGTTGATCCAGATATCGCCTTCTg         <  1:5713191/93‑1 (MQ=255)

                                                                                aaGCGCGTAGCTCTGGAACACCATCGCAATCCCGCGATCTTTTGGCTCCATTTCAGTCACGCGCTGGTCGTTGATCCAGATATCGCCTTCTGt        >  2:772620/1‑93 (MQ=255)

                                                                                 aGCGCGTAGCTCTGGAACACCATCGCAATCCCGCGATCTTTTGGCTCCATTTCAGTCACGCGCTGGTCGTTGATCCAGATATCGCCTTCTGTc       >  2:5444400/1‑93 (MQ=255)

                                                                                 aGCGCGTAGCTCTGGAACACCATCGCAATCCCGCGATCTTTTGGCTCCATTTCAGTCACGCGCTGGTCGTTGATCCAGATATCGCCTTCTGTc       >  1:3565015/1‑93 (MQ=255)

                                                                                 aGCGCGTAGCTCTGGAACACCATCGCAATCCCGCGATCTTTTGGCTCCATTTCAGTCACGCGCTGGTCGTTGATCCAGATATCGCCTTCTGTc       <  1:5285381/93‑1 (MQ=255)

                                                                                 aGCGCGTAGCTCTGGAACACCATCGCAATCCCGCGATCTTTTGGCTCCATTTCAGTCACGCGCTGGTCGTTGATCCAGATATCGCCTTCTGTc       <  1:5617572/93‑1 (MQ=255)

                                                                                 aGCGCGTAGCTCGGGAACCCCAGCGCAATCCCGCGTTTTTTTGGCTCCATTTCAGTCACGCGCTGGTCGTTGATCCAGATATCGCCTTCTGTc       <  1:3664527/93‑1 (MQ=255)

                                                                                    gcgTAGCTCTGGAACACCATCGCAATCCCGCGATCTTTTGGCTCCATTTCAGTCACGCGCTGGTCGTTGATCCAGATATCGCCTTCTGTCAcc    <  2:3858889/93‑1 (MQ=255)

                                                                                      gTAGCTCTGGAACACCATCGCAATCCCGCGATCTTTTGGCTCCATTTCAGTCACGCGCTGGTCGTTGATCCAGATATCGCCTTCTGTCACCCg  >  1:3874559/1‑93 (MQ=255)

CGCTCGGCAATTTGCTGCTTGCCCATGCCGCGAATTTTCAGCCCCCACGCCATGTTTTCTTCGACACTCATATGCGGATAAAGCGCGTAGTTCTGGAACACCATCGCAATCCCGCGATCTTTTGGCTCCATTTCAGTCACGCGCTGGTCGTTGATCCAGATATCGCCTTCTGTCACCCG  >  NC_000913/3586471‑3586649

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 19 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`