Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,590,502 A→G T189T (ACT→ACC)  b3454 ← ATP‑binding component of leucine transport

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,590,5020AG99.7% 995.1 / NA 300T189T (ACT→ACC) b3454ATP‑binding component of leucine transport
Reads supporting (aligned to +/- strand):  ref base A (0/0);  major base G (138/161);  minor base T (0/1);  total (138/162)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 302 total aligned reads displayed.

ATCGGAAAGCACTACATGGCCGTTTTCCAGCACGTAGCCGCGATCCGCCAGCTTTAGCGCCTGGTTGGCGTTCTGCTCGACGAGAAAGATAGTCATCCCCTGCTCGCGCAGCTGCTCGATGGTGTCGAAAATTTGCTGGATGATAATCGGCGCAAGACCGAGCGATGGCTCATCAAGCAG  >  NC_000913/3590412‑3590591
                                                                                          |                                                                                         
aTCGGAAAGCACTACATGGCCGTTTTCCAGCACGTAGCCGCGATCCGCCAGCTTTAGCGCCTGGTTGGCGTTCTGCTCGACGAGAAAGATGGt                                                                                         <  2:3397151/93‑1 (MQ=255)
aTCGGAAAGCACTACATGGCCGTTTTCCAGCACGTAGCCGCGATCCGCCAGCTTTAGCGCCTGGTTGGCGTTCTGCTCGACGAGAAAGATGGt                                                                                         <  1:5361106/93‑1 (MQ=255)
  cGGAAAGCACTACATGGCCGTTTTCCAGCACGTAGCCGCGATCCGCCAGCTTTAGCGCCTGGTTGGCGTTCTGCTCGACGAGAAAGATGGTCa                                                                                       <  1:2827443/93‑1 (MQ=255)
    gAAAGCACTACATGGCCGTTTTCCAGCACGTAGCCGCGATCCGCCAGCTTTAGCGCCTGGTTGGCGTTCTGCTCGACGAGAAAGATGGTCATc                                                                                     <  1:3001700/93‑1 (MQ=255)
       aGCACTACATGGCCGTTTTCCGGCACGTAGCCGCGATCCGCCAGCTTTAGCGCCTGGTTGGCGTTCTGCTCGACGAGAAAGATGGTCATcccc                                                                                  <  1:5671559/93‑1 (MQ=255)
       aGCACTACATGGCCGTTTTCCAGCACGTAGCCGCGATCCGCCAGCTTTAGCGCCTGGTTGGCGTTCTGCTCGACGAGAAAGATGGTCATcccc                                                                                  >  2:2393603/1‑93 (MQ=255)
       aGCACTACATGGCCGTTTTCCAGCACGTAGCCGCGATCCGCCAGCTTTAGCGCCTGGTTGGCGTTCTGCTCGACGAGAAAGATGGTCATcccc                                                                                  >  2:2325465/1‑93 (MQ=255)
       aGCACTACATGGCCGTTTTCCAGCACGTAGCCGCGATCCGCCAGCTTTAGCGCCTGGTTGGCGTTCTGCTCGACGAGAAAGATGGTCATcccc                                                                                  <  2:6219001/93‑1 (MQ=255)
        gCACTACATGGCCGTTTTCCAGCACGTAGCCGCGATCCGCCAGCTTTAGCGCCTGGTTGGCGTTCTGCTCGACGAGAAAGATGGTCATCCCCt                                                                                 >  1:1804936/1‑93 (MQ=255)
          ccTACATGGCCGTTTTCCAGCACGTAGCCGCGATCCGCCAGCTTTAGCGCCTGGTTGGCGTTCTGCTCGACGAGAAAGATGGTCATCCCCTGc                                                                               <  1:3896170/92‑1 (MQ=255)
          aCTACATGGCCGTTTTCCAGCACGTAGCCGCGATCCGCCAGCTTTAGCGCCTGGTTGGCGTTCTGCTCGACGAGAAAGATGGTCATCCCCTGc                                                                               >  2:564328/1‑93 (MQ=255)
          aCTACATGGCCGTTTTCCAGCACGTAGCCGCGATCCGCCAGCTTTAGCGCCTGGTTGGCGTTCTGCTCGACGAGAAAGATGGTCATCCCCTGc                                                                               <  2:2657550/93‑1 (MQ=255)
           cTACATGGCCGTTTTCCAGCACGTAGCCGCGATCCGCCAGCTTTAGCGCCTGGTTGGCGTTCTGCTCGACGAGAAAGATGGTCATCCCCTGCt                                                                              >  2:4669435/1‑93 (MQ=255)
            tACATGGCCGTTTTCCAGCACGTAGCCGCGATCCGCCAGCTTTAGCGCCTGGTTGGCGTTCTGCTCGACGAGAAAGATGGTCATCCCCTGCTc                                                                             >  1:3661218/1‑93 (MQ=255)
             tcATGGCCGTTTTCCAGCACGTAGCCGCGATCCGCCAGCTTTAGCGCCTGGTTGGCGTTCTGCTCGACGAGAAAGATGGTCATCCCCTGCTcg                                                                            <  2:4839227/92‑1 (MQ=255)
             aCATGGCCGTTTTCCAGCACGTAGCCGCGATCCGCCAGCTTTAGCGCCTGGTTGGCGTTCTGCTCGACGAGAAAGATGGTCATCCCCTGCTcg                                                                            >  2:5729336/1‑93 (MQ=255)
              cATGGCCGTTTTCCAGCACGTAGCCGCGATCCGCCAGCTTTAGCGCCTGGTTGGCGTTCTGCTCGACGAGAAAGATGGTCATCCCCTGCTcgc                                                                           <  2:4051954/93‑1 (MQ=255)
               aTGGCCGTTTTCCAGCACGTAGCCGCGATCCGCCAGCTTTAGCGCCTGGTTGGCGTTCTGCTCGACGAGAAAGATGGTCATCCCCTGCTcgcg                                                                          >  2:6143059/1‑93 (MQ=255)
                 ggCCGTTTTCCAGCACGTAGCCGCGATCCGCCAGCTTTAGCGCCTGGTTGGCGTTCTGCTCGACGAGAAAGATGGTCATCCCCTGCTCGCGCa                                                                        <  2:699709/93‑1 (MQ=255)
                 ggCCGTTTTCCAGCACGTAGCCGCGATCCGCCAGCTTTAGCGCCTGGTTGGCGTTCTGCTCGACGAGAAAGATGGTCATCCCCTGCTCGCGCa                                                                        <  1:1542589/93‑1 (MQ=255)
                    cGTTTTCCAGCCCGTAGCCGCGATCCGCCAGCTTTAGCGCCTGGTTGGCGTTCTGCTCGACGAGAAAGATGGTCATCCCCTGCTCGCGCAgct                                                                     <  2:3577648/93‑1 (MQ=255)
                    cGTTTTCCAGCACGTAGCCGCGATCCGCCAGCTTTAGCGCCTGGTTGGCGTTCTGCTCGACGAGAAAGATGGTCATCCCCTGCTCGCGCAgct                                                                     >  2:3680424/1‑93 (MQ=255)
                    cGTTTTCCAGCACGTAGCCGCGATCCGCCAGCTTTAGCGCCTGGTTGGCGTTCTGCTCGACGAGAAAGATGGTCATCCCCTGCTCGCGCAgct                                                                     >  2:5342587/1‑93 (MQ=255)
                    cGTTTTCCAGCACGTAGCCGCGATCCGCCAGCTTTAGCGCCTGGTTGGCGTTCTGCTCGACGAGAAAGATGGTCATCCCCTGCTCGCGCAgct                                                                     <  1:3764306/93‑1 (MQ=255)
                    cGTTTTCCAGCACGTAGCCGCGATCCGCCAGCTTTAGCGCCTGGTTGGCGTTCTGCTCGACGAGAAAGATGGTCATCCCCTGCTCGCGCAgct                                                                     <  1:4066438/93‑1 (MQ=255)
                     gTTTTCCAGCACGTAGCCGCGATCCGCCAGCTTTAGCGCCTGGTTGGCGTTCTGCTCGACGAGAAAGATGGTCATCCCCTGCTCGCGCAgctg                                                                    <  2:1478516/93‑1 (MQ=255)
                     gTTTTCCAGCACGTAGCCGCGATCCGCCAGCTTTAGCGCCTGGTTGGCGTTCTGCTCGACGAGAAAGATGGTCATCCCCTGCTCGCGCAgctg                                                                    >  1:1709886/1‑93 (MQ=255)
                      ttttCCAGCACGTAGCCGCGATCCGCCAGCTTTAGCGCCTGGTTGGCGTTCTGCTCGACGAGAAAGATGGTCATCCCCTGCTCGCGCAgctgc                                                                   <  1:2621216/93‑1 (MQ=255)
                       tttCCAGCACGTAGCCGCGATCCGCCAGCTTTAGCGCCTGGTTGGCGTTCTGCTCGACGAGAAAGATGGTCATCCCCTGCTCGCGCAgctgct                                                                  <  1:4238173/93‑1 (MQ=255)
                          ccAGCACGTAGCCGCGATCCGCCAGCTTTAGCGCCTGGTTGGCGTTCTGCTCGACGAGAAAGATGGTCATCCCCTGCTCGCGCAGCTGCTCGa                                                               >  1:5921262/1‑93 (MQ=255)
                           cAGCACGTAGCCGCGATCCGCCAGCTTTAGCGCCTGGTTGGCGTTCTGCTCGACGAGAAAGATGGTCATCCCCTGCTCGCGCAGCTGCTCGAt                                                              <  2:6622600/93‑1 (MQ=255)
                           cAGCACGTAGCCGCGATCCGCCAGCTTTAGCGCCTGGTTGGCGTTCTGCTCGACGAGAAAGATGGTCATCCCCTGCTCGCGCAGCTGCTCGAt                                                              >  1:3281868/1‑93 (MQ=255)
                            aGCACGTAGCCGCGATCCGCCAGCTTTAGCGCCTGGTTGGCGTTCTGCTCGACGAGAAAGATGGTCATCCCCTGCTCGCGCAGCTGCTCGATg                                                             >  1:721739/1‑93 (MQ=255)
                            aGCACGTAGCCGCGATCCGCCAGCTTTAGCGCCTGGTTGGCGTTCTGCTCGACGAGAAAGATGGTCATCCCCTGCTCGCGCAGCTGCTCGATg                                                             >  1:6655006/1‑93 (MQ=255)
                             gCACGTAGCCGCGATCCGCCAGCTTTAGCGCCTGGTTGGCGTTCTGCTCGACGAGAAAGATGGTCATCCCCTGCTCGCGCAGCTGCTCGATgg                                                            >  2:5963401/1‑93 (MQ=255)
                             gCACGTAGCCGCGATCCGCCAGCTTTAGCGCCTGGTTGGCGTTCTGCTCGACGAGAAAGATGGTCATCCCCTGCTCGCGCAGCTGCTCGATgg                                                            >  2:4208698/1‑93 (MQ=255)
                               aCGTAGCCGCGATCCGCCAGCTTTAGCGCCTGGTTGGCGTTCTGCTCGACGAGAAAGATGGTCATCCCCTGCTCGCGCAGCTGCTCGATGgtg                                                          <  1:3697400/93‑1 (MQ=255)
                               aCGTAGCCGCGATCCGCCAGCTTTAGCGCCTGGTTGGCGTTCTGCTCGACGAGAAAGATGGTCATCCCCTGCTCGCGCAGCTGCTCGATGgtg                                                          >  1:4842746/1‑93 (MQ=255)
                               aCGTAGCCGCGATCCGCCAGCTTTAGCGCCTGGTTGGCGTTCTGCTCGACGAGAAAGATGGTCATCCCCTGCTCGCGCAGCTGCTCGATGgtg                                                          <  1:913427/93‑1 (MQ=255)
                                cGTAGCCGCGATCCGCCAGCTTTAGCGCCTGGTTGGCGTTCTGCTCGACGAGAAAGATGGTCATCCCCTGCTCGCGCAGCTGCTCGATGgtgt                                                         >  2:5492085/1‑93 (MQ=255)
                                cGTAGCCGCGATCCGCCAGCTTTAGCGCCTGGTTGGCGTTCTGCTCGACGAGAAAGATGGTCATCCCCTGCTCGCGCAGCTGCTCGATGgtgt                                                         <  2:3229686/93‑1 (MQ=255)
                                 gTAGCCGCGATCCGCCAGCTTTAGCGCCTGGTTGGCGTTCTGCTCGACGAGAAAGATGGTCATCCCCTGCTCGCGCAGCTGCTCGATGGTGTc                                                        >  2:4249476/1‑93 (MQ=255)
                                   aGCCGCGATCCGCCAGCTTTAGCGCCTGGTTGGCGTTCTGCTCGACGAGAAAGATGGTCATCCCCTGCTCGCGCAGCTGCTCGATGGTGTCGa                                                      <  1:3570322/93‑1 (MQ=255)
                                   aGCCGCGATCCGCCAGCTTTAGCGCCTGGTTGGCGTTCTGCTCGACGAGAAAGATGGTCATCCCCTGCTCGCGCAGCTGCTCGATGGTGTCGa                                                      <  2:669785/93‑1 (MQ=255)
                                   aGCCGCGATCCGCCAGCTTTAGCGCCTGGTTGGCGTTCTGCTCGACGAGAAAGATGGTCATCCCCTGCTCGCGCAGCTGCTCGATGGTGTCGa                                                      >  1:5871940/1‑93 (MQ=255)
                                      cgcgATCCGCCAGCTTTAGCGCCTGGTTGGCGTTCTGCTCGACGAGAAAGATGGTCATCCCCTGCTCGCGCAGCTGCTCGATGGTGTCTaaaa                                                   <  1:3260908/93‑1 (MQ=255)
                                       gcgATCCGCCAGCTTTAGCGCCTGGTTGGCGTTCTGCTCGACGAGAAAGATGGTCATCCCCTGCTCGCGCAGCTGCTCGATGGTGTCGAAAAt                                                  <  2:2509409/93‑1 (MQ=255)
                                        cgATCCGCCAGCTTTAGCGCCTGGTTGGCGTTCTGCTCGACGAGAAAGATGGTCATCCCCTGCTCGCGCAGCTGCTCGATGGTGTCGAAAAtt                                                 <  2:2882610/93‑1 (MQ=255)
                                        cgATCCGCCAGCTTTAGCGCCTGGTTGGCGTTCTGCTCGACGAGAAAGATGGTCATCCCCTGCTCGCGCAGCTGCTCGATGGTGTCGAAAAtt                                                 <  1:2690146/93‑1 (MQ=255)
                                         gATCCGCCAGCTTTAGCGCCTGGTTGGCGTTCTGCTCGACGAGAAAGATGGTCATCCCCTGCTCGCGCAGCTGCTCGATGGTGTCGAAAAttt                                                >  1:4627935/1‑93 (MQ=255)
                                         gATCCGCCAGCTTTAGCGCCTGGTTGGCGTTCTGCTCGACGAGAAAGATGGTCATCCCCTGCTCGCGCAGCTGCTCGATGGTGTCGAAAAttt                                                >  1:5789500/1‑93 (MQ=255)
                                          aTCCGCCAGCTTTAGCGCCTGGTTGGCGTTCTGCTCGACGAGAAAGATGGTCATCCCCTGCTCGCGCAGCTGCTCGATGGTGTCGAAAATTTg                                               >  2:2612312/1‑93 (MQ=255)
                                          aTCCGCCAGCTTTAGCGCCTGGTTGGCGTTCTGCTCGACGAGAAAGATGGTCATCCCCTGCTCGCGCAGCTGCTCGATGGTGTCGAAAATTTg                                               >  1:1290815/1‑93 (MQ=255)
                                           tCCGCCAGCTTTAGCGCCTGGTTGGCGTTCTGCTCGACGAGAAAGATGGTCATCCCCTGCTCGCGCAGCTGCTCGGTGGTGTCGAAAATTTGc                                              >  1:3517975/1‑93 (MQ=255)
                                            ccGCCAGCTTTAGCGCCTGGTTGGCGTTCTGCTCGACGAGAAAGATGGTCATCCCCTGCTCGCGCAGCTGCTCGATGGTGTCGAAAATTTGCt                                             <  2:1341472/93‑1 (MQ=255)
                                            ccGCCAGCTTTAGCGCCTGGTTGGCGTTCTGCTCGACGAGAAAGATGGTCATCCCCTGCTCGCGCAGCTGCTCGATGGTGTCGAAAATTTGCt                                             <  1:3359590/93‑1 (MQ=255)
                                              gCCAGCTTTAGCGCCTGGTTGGCGTTCTGCTCGACGAGAAAGATGGTCATCCCCTGCTCGCGCAGCTGCTCGATGGTGTCGAAAATTTGCTgg                                           <  1:5542096/93‑1 (MQ=255)
                                                cAGCTTTAGCGCCTGGTTGGCGTTCTGCTCGACGAGAAAGATGGTCATCCCCTGCTCGCGCAGCTGCTCGATGGTGTCGAAAATTTGCTGgat                                         >  1:1272686/1‑93 (MQ=255)
                                                cAGCTTTAGCGCCTGGTTGGCGTTCTGCTCGACGAGAAAGATGGTCATCCCCTGCTCGCGCAGCTGCTCGATGGTGTCGAAAATTTGCTGgat                                         <  1:309987/93‑1 (MQ=255)
                                                  gCTTTAGCGCCTGGTTGGCGTTCTGCTCGACGAGAAAGATGGTCATCCCCTGCTCGCGCAGCTGCTCGATGGTGTCGAAAATTTGCTGgatga                                       <  1:20794/93‑1 (MQ=255)
                                                  gCTTTAGCGCCTGGTTGGCGTTCTGCTCGACGAGAAAGATGGTCATCCCCTGCTCGCGCAGCTGCTCGATGGTGTCGAAAATTTGCTGgatga                                       <  1:332184/93‑1 (MQ=255)
                                                    tttAGCGCCTGGTTGGCGTTCTGCTCGACGAGAAAGATGGTCATCCCCTGCTCGCGCAGCTGCTCGATGGTGTCGAAAATTTGCTGGATGATa                                     >  2:6525700/1‑93 (MQ=255)
                                                    tttAGCGCCTGGTTGGCGTTCTGCTCGACGAGAAAGATGGTCATCCCCTGCTCGCGCAGCTGCTCGATGGTGTCGAAAATTTGCTGGATGATa                                     >  2:3104953/1‑93 (MQ=255)
                                                     ttAGCGCCTGGTTGGCGTTCTGCTCGACGAGAAAGATGGTCATCCCCTGCTCGCGCAGCTGCTCGATGGTGTCGAAAATTTGCTGGATGATaa                                    <  1:159907/93‑1 (MQ=255)
                                                       aGCGCCTGGTTGGCGTTCTGCTCGACGAGAAAGATGGTCATCCCCTGCTCGCGCAGCTGCTCGATGGTGTCGAAAATTTGCTGGATGATAATc                                  <  1:4563575/93‑1 (MQ=255)
                                                         cgcCTGGTTGGCGTTCTGCTCGACGAGAAAGATGGTCATCCCCTGCTCGCGCAGCTGCTCGATGGTGTCGAAAATTTGCTGGATGATAATCgg                                <  2:206894/93‑1 (MQ=255)
                                                          gcCTGGTTGGCGTTCTGCTCGACGAGAAAGATGGTCATCCCCTGCTCGCGCAGCTGCTCGATGGTGTCGAAAATTTGCTGGATGATAATCGgc                               <  2:1635283/93‑1 (MQ=255)
                                                            cTGGTTGGCGTTCTGCTCGACGAGAAAGATGGTCATCCCCTGCTCGCGCAGCTGCTCGATGGTGTCGAAAATTTGCTGGATGATAATCGgcgc                             <  1:1999940/93‑1 (MQ=255)
                                                            cTGGTTGGCGTTCTGCTCGACGAGAAAGATGGTCATCCCCTGCTCGCGCAGCTGCTCGATGGTGTCGAAAATTTGCTGGATGATAATCGgcgc                             >  1:5061844/1‑93 (MQ=255)
                                                              ggTTGGCGTTCTGCTCGACGAGAAAGATGGTCATCCCCTGCTCGCGCAGCTGCTCGATGGTGTCGAAAATTTGCTGGATGATAATCGGCGCaa                           <  2:1120144/93‑1 (MQ=255)
                                                              ggTTGGCGTTCTGCTCGACGAGAAAGATGGTCATCCCCTGCTCGCGCAGCTGCTCGATGGTGTCGAAAATTTGCTGGATGATAATCGGCGCaa                           <  2:1522817/93‑1 (MQ=255)
                                                               gTTGGCGTTCTGCTCGACGAGAAAGATGGTCATCCCCTGCTCGCGCAGCTGCTCGATGGTGTCGAAAATTTGCTGGATGATAATCGGCGCAAg                          >  2:6034935/1‑93 (MQ=255)
                                                               gTTGGCGTTCTGCTCGACGAGAAAGATGGTCATCCCCTGCTCGCGCAGCTGCTCGATGGTGTCGAAAATTTGCTGGATGATAATCGGCGCAAg                          >  1:4352988/1‑93 (MQ=255)
                                                                 tGGCGTTCTGCTCGACGAGAAAGATGGTCATCCCCTGCTCGCGCAGCTGCTCGATGGTGTCGAAAATTTGCTGGATGATAATCGGCGCAAGAc                        >  1:230688/1‑93 (MQ=255)
                                                                  ggCGTTCTGCTCGACGAGAAAGATGGTCATCCCCTGCTCGCGCAGCTGCTCGATGGTGTCGAAAATTTGCTGGATGATAATCGGCGCAAGAcc                       >  1:1037427/1‑93 (MQ=255)
                                                                  ggCGTTCTGCTCGACGAGAAAGATGGTCATCCCCTGCTCGCGCAGCTGCTCGATGGTGTCGAAAATTTGCTGGATGATAATCGGCGCAAGAcc                       >  1:6450589/1‑93 (MQ=255)
                                                                   gCGTTCTGCTCGACGAGAAAGATGGTCATCCCCTGCTCGCGCAGCTGCTCGATGGTGTCGAAAATTTGCTGGATGATAATCGGCGCAAGACCg                      <  2:3886497/93‑1 (MQ=255)
                                                                   gCGTTCTGCTCGACGAGAAAGATGGTCATCCCCTGCTCGCGCAGCTGCTCGATGGTGTCGAAAATTTGCTGGATGATAATCGGCGCAAGACCg                      <  2:1896623/93‑1 (MQ=255)
                                                                     gTTCTGCTCGACGAGAAAGATGGTCATCCCCTGCTCGCGCAGCTGCTCGATGGTGTCGAAAATTTGCTGGATGATAATCGGCGCAAGACCGAg                    <  1:4680256/93‑1 (MQ=255)
                                                                      ttCTGCTCGACGAGAAAGATGGTCATCCCCTGCTCGCGCAGCTGCTCGATGGTGTCGAAAATTTGCTGGATGATAATCGGCGCAAGACCGAGc                   >  2:2149696/1‑93 (MQ=255)
                                                                      ttCTGCTCGACGAGAAAGATGGTCATCCCCTGCTCGCGCAGCTGCTCGATGGTGTCGAAAATTTGCTGGATGATAATCGGCGCAAGACCGAGc                   >  1:518048/1‑93 (MQ=255)
                                                                         tGCTCGACGAGAAAGATGGTCATCCCCTGCTCGCGCAGCTGCTCGATGGTGTCGAAAATTTGCTGGATGATAATCGGCGCAAGACCGAGCGAt                >  2:1499937/1‑93 (MQ=255)
                                                                         tGCTCGACGAGAAAGATGGTCATCCCCTGCTCGCGCAGCTGCTCGATGGTGTCGAAAATTTGCTGGATGATAATCGGCGCAAGACCGAGCGAt                <  2:81805/93‑1 (MQ=255)
                                                                         tGCTCGACGAGAAAGATGGTCATCCCCTGCTCGCGCAGCTGCTCGATGGTGTCGAAAATTTGCTGGATGATAATCGGCGCAAGACCGAGCGAt                <  2:4945359/93‑1 (MQ=255)
                                                                         tGCTCGACGAGAAAGATGGTCATCCCCTGCTCGCGCAGCTGCTCGATGGTGTCGAAAATTTGCTGGATGATAATCGGCGCAAGACCGAGCGAt                >  2:325884/1‑93 (MQ=255)
                                                                         tGCTCGACGAGAAAGATGGTCATCCCCTGCTCGCGCAGCTGCTCGATGGTGTCGAAAATTTGCTGGATGATAATCGGCGCAAGACCGAGCGAt                >  2:6362120/1‑93 (MQ=255)
                                                                         tGCTCGACGAGAAAGATGGTCATCCCCTGCTCGCGCAGCTGCTCGATGGTGTCGAAAATTTGCTGGATGATAATCGGCGCAAGACCGAGCGAt                >  2:938041/1‑93 (MQ=255)
                                                                         tGCGCGACGAGAAAGATGGTCATCCCCTGCGCGCGCAGCTGCTCGATGGTGTCGAAAATTTGCTGGATGATAATCGGCGCAAGACCGAGCGAt                <  2:2755712/93‑1 (MQ=255)
                                                                          gCTCGACGAGAAAGATGGTCATCCCCTGCTCGCGCAGCTGCTCGATGGTGTCGAAAATTTGCTGGATGATAATCGGCGCAAGACCGAGCGATg               <  2:3994183/93‑1 (MQ=255)
                                                                           cTCGACGAGAAAGATGGTCATCCCCTGCTCGCGCAGCTGCTCGATGGTGTCGAAAATTTGCTGGATGATAATCGGCGCAAGACCGAGCGATgg              <  1:3954970/93‑1 (MQ=255)
                                                                            tCGACGAGAAAGATGGTCATCCCCTGCTCGCGCAGCTGCTCGATGGTGTCGAAAATTTGCTGGATGATAATCGGCGCAAGACCGAGCGATGGc             <  2:1375832/93‑1 (MQ=255)
                                                                            tCGACGAGAAAGATGGTAATCCCCTGCTCGCGCAGCTGCTCGATGGTGTCGAAAATTTGCTGGATGATAATCGGCGCAAGACCGAGCGATGGc             >  2:5175711/1‑93 (MQ=255)
                                                                                  agaAAGATGGTCATCCCCTGCTCGCGCAGCTGCTCGATGGTGTCGAAAATTTGCTGGATGATAATCGGCGCAAGACCGAGCGATGGCtcatca       <  1:6063641/93‑1 (MQ=255)
                                                                                   gaAAGATGGTCATCCCCTGCTCGCGCAGCTGCTCGATGGTGTCGAAAATTTGCTGGATGATAATCGGCGCAAGACCGAGCGATGGCTCATCaa      >  2:4986316/1‑93 (MQ=255)
                                                                                   gaAAGATGGTCATCCCCTGCTCGCGCAGCTGCTCGATGGTGTCGAAAATTTGCTGGATGATAATCGGCGCAAGACCGAGCGATGGCTCATCaa      <  2:4486219/93‑1 (MQ=255)
                                                                                     aaGATGGTCATCCCCTGCTCGCGCAGCTGCTCGATGGTGTCGAAAATTTGCTGGATGATAATCGGCGCAAGACCGAGCGATGGCTCATCAAGc    >  1:185132/1‑93 (MQ=255)
                                                                                     aaGATGGTCATCCCCTGCTCGCGCAGCTGCTCGATGGTGTCGAAAATTTGCTGGATGATAATCGGCGCAAGACCGAGCGATGGCTCATCAAGc    >  2:3495614/1‑93 (MQ=255)
                                                                                      aGATGGTCATCCCCTGCTCGCGCAGCTGCTCGATGGTGTCGAAAATTTGCTGGATGATAATCGGCGCAAGACCGAGCGATGGCTCATCAAGCa   >  1:1183543/1‑93 (MQ=255)
                                                                                      aGATGGTCATCCACTGCTCGCGCAGCTGCTCGATGGTGTCGAAAATTTGCTGGATGATAATCGGCGCAAGACCGAGCGATGGCTCATCAAGCa   >  2:1015005/1‑93 (MQ=255)
                                                                                       gATGGTCATCCCCTGCTCGCGCAGCTGCTCGATGGTGTCGAAAATTTGCTGGATGATAATCGGCGCAAGACCGAGCGATGGCTCATCAAGCAg  >  2:1220935/1‑93 (MQ=255)
                                                                                          |                                                                                         
ATCGGAAAGCACTACATGGCCGTTTTCCAGCACGTAGCCGCGATCCGCCAGCTTTAGCGCCTGGTTGGCGTTCTGCTCGACGAGAAAGATAGTCATCCCCTGCTCGCGCAGCTGCTCGATGGTGTCGAAAATTTGCTGGATGATAATCGGCGCAAGACCGAGCGATGGCTCATCAAGCAG  >  NC_000913/3590412‑3590591

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑