BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,634,228	A→G	R271R (CGT→CGC)	yhiN ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,634,228	0	A	G	99.6%	894.3 / ‑5.1	279	R271R (CGT→CGC)	yhiN	hypothetical protein
Reads supporting (aligned to +/- strand): ref base A (0/1); new base G (138/140); total (138/141)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.08e-01

Only 100 of 288 total aligned reads displayed.

GATTTGCCCGAGTTGCTGTAAGCGTTCAACCAACCGCTTCGGTAGATGAACCGCCAGTGTGTTTTTCAGGCTTTGATTCGGATGTGCGTTACGCTGCTCATTCAGGAAGGTTTCGAGGTCCACATCCGGTAGCAGATTGATGCTGACAAATTCCCCCGGTTGCCAGTAGCTTGAAATCTG  >  NC_000913/3634138‑3634317

gATTTGCCCGAGTTGCTGTAAGCGTTCAACCAACCGCTTCGGTAGATGAACCGCCAGTGTGTTTTTCAGGCTTTGATTCGGATGTGCGTTGCg                                                                                         >  1:2298335/1‑93 (MQ=255)

 aTTTGCCCGAGTTGCTGTAAGCGTTCAACCAACCGCTTCGGTAGATGAACCGCCAGTGTGTTTTTCAGGCTTTGATTCGGATGTGCGTTgcgc                                                                                        >  1:5027335/1‑93 (MQ=255)

  tttGCCCGAGTTGCTGTAAGCGTTCAACCAACCGCTTCGGTAGATGAACCGCCAGTGTGTTTTTCAGGCTTTGATTCGGATGTGCGTTGCgct                                                                                       >  1:2850400/1‑93 (MQ=255)

        cGAGTTGCTGTAAGCGTTCAACCAACCGCTTCGGTAGATGAACCGCCAGTGTGTTTTTCAGGCTTTGATTCGGATGTGCGTTGCGCTGCTCAt                                                                                 <  1:5972426/93‑1 (MQ=255)

          aGTTGCTGTAAGCGTTCAACCAACCGCTTCGGTAGATGAACCGCCAGTGTGTTTTTCAGGCTTTGATTCGGATGTGCGTTGCGCTGCTCATTc                                                                               >  2:6182841/1‑93 (MQ=255)

           gTTGCTGTAAGCGTTCAACCAACCGCTTCGGTAGATGAACCGCCAGTGTGCTTTTCAGGCTTTGATTCGGATGTGCGTTGCGCTGCTCATTCa                                                                              >  1:1009636/1‑93 (MQ=255)

            ttGCTGTAAGCGTTCAACCAACCGCTTCGGTAGATGAACCGCCAGTGTGTTTTTCAGGCTTTGATTCGGATGTGCGTTGCGCTGCTCATTCAg                                                                             <  1:720515/93‑1 (MQ=255)

            ttGCTGTAAGCGTTCAACCAACCGCTTCGGTAGATGAACCGCCAGTGTGTTTTTCAGGCTTTGATTCGGATGTGCGTTGCGCTGCTCATTCAg                                                                             <  2:1848559/93‑1 (MQ=255)

              gCTGTAAGCGTTCAACCAACCGCTTCGGTAGATGAACCGCCAGTGTGTTTTTCAGGCTTTGATTCGGATGTGCGTTGCGCTGCTCATTCAGGa                                                                           <  2:2164334/93‑1 (MQ=255)

               cTGTAAGCGTTCAACCAACCGCTTCGGTAGATGAACCGCCAGTGTGTTTTTCAGGCTTTGATTCGGATGTGCGTTGCGCTGCTCATTCAGGaa                                                                          <  2:4217306/93‑1 (MQ=255)

                tGTAAGCGTTCAACCAACCGCTTCGGTAGATGAACCGCCAGTGTGTTTTTCAGGCTTTGATTCGGATGTGCGTTGCGCTGCTCATTCAGGAAg                                                                         <  1:1138840/93‑1 (MQ=255)

                tGTAAGCGTTCAACCAACCGCTTCGGTAGATGAACCGCCAGTGTGTTTTTCAGGCTTTGATTCGGATGTGCGTTGCGCTGCTCATTCAGGAAg                                                                         <  1:3915319/93‑1 (MQ=255)

                tGTAAGCGTTCAACCAACCGCTTCGGTAGATGAACCGCCAGTGTGTTTTTCAGGCTTTGATTCGGATGTGCGTTGCGCTGCTCATTCAGGAAg                                                                         <  2:3061410/93‑1 (MQ=255)

                tGTAAGCGTTCAACCAACCGCTTCGGTAGATGAACCGCCAGTGTGTTTTTCAGGCTTTGATTCGGATGTGCGTTGCGCTGCTCATTCAGGAAg                                                                         <  2:4125294/93‑1 (MQ=255)

                tGTAACGGTTCAACCAACCGCTTCGGTAGATGAACCGCCAGTGTGTTTTTCAGGCTTTGATTCGGATGTGCGTTGCGCTGCTCATTCAGGAAg                                                                         <  2:1210695/93‑1 (MQ=255)

                 gTAAGCGTTCAACCAACCGCTTCGGTAGATGAACCGCCAGTGTGTTTTTCAGGCTTTGATTCGGATGTGCGTTGCGCTGCTCATTCAGGAAgg                                                                        >  1:4281487/1‑93 (MQ=255)

                  tAAGCGTTCAACCAACGGTTTCGGTCGATGAACCGCCAGTGTGTTTTTCAGGCTTTGATTCGGATGTGCGTTGCGCTGCTCATTCAGGAAGGt                                                                       <  1:3721569/93‑1 (MQ=255)

                  tAAGCGTTCAACCAACCGCTTCGGTAGATGAACCGCCAGTGTGTTTTTCAGGCTTTGATTCGGATGTGCGTTGCGCTGCTCATTCAGGAAGGt                                                                       >  2:2770777/1‑93 (MQ=255)

                  tAAGCGTTCAACCAACCGCTTCGGTAGATGAACCGCCAGTGTGTTTTTCAGGCTTTGATTCGGATGTGCGTTGCGCTGCTCATTCAGGAAGGt                                                                       <  1:1640223/93‑1 (MQ=255)

                  tAAGCGTTCAACCAACCGCTTCGGTAGATGAACCGCCAGTGTGTTTTTCAGGCTTTGATTCGGATGTGCGTTGCGCTGCTCATTCAGGAAGGt                                                                       <  2:3552229/93‑1 (MQ=255)

                   aaGCGTTCAACCAACCGCTTCGGTAGATGAACCGCCAGTGTGTTTTTCAGGCTTTGATTCGGATGTGCGTTGCGCTGCTCATTCAGGAAGGtt                                                                      >  1:1935627/1‑93 (MQ=255)

                   aaGCGTTCAACCAACCGCTTCGGTAGATGAACCGCCAGTGTGTTTTTCAGGCTTTGATTCGGATGTGCGTTGCGCTGCTCATTCAGGAAGGtt                                                                      >  1:4827411/1‑93 (MQ=255)

                    aGCGTTCAACCAACCGCTTCGGTAGATGAACCGCCAGTGTGTTTTTCAGGCTTTGATTCGGATGTGCGTTGCGCTGCTCATTCAGGAAGGttt                                                                     <  2:1303668/93‑1 (MQ=255)

                     gCGTTCAACCAACCGCTTCGGTAGATGAACCGCCAGTGTGTTTTTCAGGCTTTGATTCGGATGTGCGTTGCGCTGCTCATTCAGGAAGGTTTc                                                                    >  2:4528201/1‑93 (MQ=255)

                       gTTCAACCAACCGCTTCGGTAGATGAACCGCCAGTGTGTTTTTCAGGCTTTGATTCGGATGTGCGTTGCGCTGCTCATTCAGGAAGGTTTCGa                                                                  <  1:1768623/93‑1 (MQ=255)

                        ttCAACCAACCGCTTCGGTAGATGAACCGCCAGTGTGTTTTTCAGGCTTTGATTCGGATGTGCGTTGCGCTGCTCATTCAGGAAGGTTTCGAg                                                                 >  1:5918304/1‑93 (MQ=255)

                        ttCAACCAACCGCTTCGGTAGATGAACCGCCAGTGTGTTTTTCAGGCTTTGATTCGGATGTGCGTTGCGCTGCTCATTCAGGAAGGTTTCGAg                                                                 <  1:3230325/93‑1 (MQ=255)

                         tCAACCAACCGCTTCGGTAGATGAACCGCCAGTGTGTTTTTCAGGCTTTGATTCGGATGTGCGTTGCGCTGCTCATTCAGGAAGGTTTCGAgg                                                                >  1:6670484/1‑93 (MQ=255)

                         tCAACCAACCGCTTCGGTAGATGAACCGCCAGTGTGTTTTTCAGGCTTTGATTCGGATGTGCGTTGCGCTGCTCATTCAGGAAGGTTTCGAgg                                                                >  2:1067471/1‑93 (MQ=255)

                         tCAACCAACCGCTTCGGTAGATGAACCGCCAGTGTGTTTTTCAGGCTTTGATTCGGATGTGCGTTGCGCTGCTCATTCAGGAAGGTTTCGAgg                                                                <  2:1455907/93‑1 (MQ=255)

                         tCAACCAACCGCTTCGGTAGATGAACCGCCAGTGTGTTTTTCAGGCTTTGATTCGGATGTGCGTTGCGCTGCTCATTCAGGAAGGTTTCGAgg                                                                >  2:2339650/1‑93 (MQ=255)

                            accaaccGCTTCGGTAGATGAACCGCCAGTGTGTTTTTCAGGCTTTGATTCGGATGTGCGTTGCGCTGCTCATTCAGGAAGGTTTCGAGGTcc                                                             >  2:533996/1‑93 (MQ=255)

                            accaaccGCTTCGGTAGATGAACCGCCAGTGTGTTTTTCAGGCTTTGATTCGGATGTGCGTTGCGCTGCTCATTCAGGAAGGTTTCGAGGTcc                                                             >  2:3820413/1‑93 (MQ=255)

                            accaaccGCTTCGGTAGATGAACCGCCAGTGTGTTTTTCAGGCTTTGATTCGGATGTGCGTTGCGCTGCTCATTCAGGAAGGTTTCGAGGTcc                                                             >  1:2524118/1‑93 (MQ=255)

                                 ccGCTTCGGTAGATGAACCGCCAGTGTGTTTTTCAGGCTTTGATTCGGATGTGCGTTGCGCTGCTCATTCAGGAAGGTTTCGAGGTCCACATc                                                        <  2:6589402/93‑1 (MQ=255)

                                  cGCTTCGGTAGATGAACCGCCAGTGTGTTTTTCAGGCTTTGATTCGGATGTGCGTTGCGCTGCTCATTCAGGAAGGTTTCGAGGTCCACATcc                                                       <  2:5359976/93‑1 (MQ=255)

                                  cGCTTCGGTAGATGAACCGCCAGTGTGTTTTTCAGGCTTTGATTCGGATGTGCGTTGCGCTGCTCATTCAGGAAGGTTTCGAGGTCCACATcc                                                       <  2:252445/93‑1 (MQ=255)

                                   gCTTCGGTAGATGAACCGCCAGTGTGTTTTTCAGGCTTTGATTCGGATGTGCGTTGCGCTGCTCATTCAGGAAGGTTTCGAGGTCCACATCCg                                                      >  1:3385050/1‑93 (MQ=255)

                                    cTTCGGTAGATGGACCGCCAGTGTGTTTTTCAGGCTTTGATTCGGATGTGGGTTGCGCTGCTCATTGAGGAAGGTTTCGAGGTCCACATCCgg                                                     <  2:6133285/93‑1 (MQ=255)

                                    cTTCGGTAGATGAACCGCCAGTGTGTTTTTCAGGCTTTGATTCGGATGTGCGTTGCGCTGCTCATTCAGGAAGGTTTCGAGGTCCACATCCgg                                                     >  2:2096301/1‑93 (MQ=255)

                                    cTTCGGTAGATGAACCGCCAGTGTGTTTTTCAGGCTTTGATTCGGATGTGCGTTGCGCTGCTCATTCAGGAAGGTTTCGAGGTCCACATCCgg                                                     <  2:48588/93‑1 (MQ=255)

                                     ttCGGTAGATGAACCGCCAGTGTGTTTTTCAGGCTTTGATTCGGATGTGCGTTGCGCTGCTCATTCAGGAAGGTTTCGAGGTCCACATCCGGt                                                    >  2:92153/1‑93 (MQ=255)

                                     ttCGGTAGATGAACCGCCAGTGTGTTTTTCAGGCTTTGATTCGGATGTGCGTTGCGCTGCTCATTCAGGAAGGTTTCGAGGTCCACATCCGGt                                                    >  2:3172335/1‑93 (MQ=255)

                                     ttCGGTAGATGAACCGCCAGTGTGTTTTTCAGGCTTTGATTCGGATGTGCGTTGCGCTGCTCATTCAGGAAGGTTTCGAGGTCCACATCCGGt                                                    <  2:6350646/93‑1 (MQ=255)

                                      tCGGTAGATGAACCGCCAGTGTGTTTTTCAGGCTTTGATTCGGATGTGCGTTGCGCTGCTCATTCAGGAAGGTTTCGAGGTCCACATCCGGTa                                                   <  1:807783/93‑1 (MQ=255)

                                        ggTAGATGAACCGCCAGTGTGTTTTTCAGGCTTTGATTCGGATGTGCGTTACGCTGCTCATTCAGGAAGGTTTCGAGGTCCACATCCGGTAGc                                                 <  2:4988529/93‑1 (MQ=255)

                                         gTAGATGAACCGCCAGTGTGTTTTTCAGGCTTTGATTCGGATGTGCGTTGCGCTGCTCATTCAGGAAGGTTTCGAGGTCCACATCCGGTAGCa                                                >  2:1624998/1‑93 (MQ=255)

                                            gATGAACCGCCAGTGTGTTTTTCAGGCTTTGATTCGGATGTGCGTTGCGCTGCTCATTCAGGAAGGTTTCGAGGTCCACATCCGGTAGCAGAt                                             >  2:2893695/1‑93 (MQ=255)

                                             aTGAACCGCCAGTGTGTTTTTCAGGCTTTGATTCGGATGTGCGTTGCGCTGCTCATTCAGGAAGGTTTCGAGGTCCACATCCGGTAGCAGAtt                                            <  2:6199087/93‑1 (MQ=255)

                                               gAACCGCCAGTGTGTTTTTCAGGCTTTGATTCGGATGTGCGTTGCGCTGCTCATTCAGGAAGGTTTCGAGGTCCACATCCGGTAGCAGATTGa                                          >  1:5354907/1‑93 (MQ=255)

                                               gAACCGCCAGTGTGTTTTTCAGGCTTTGATTCGGATGTGCGTTGCGCTGCTCATTCAGGAAGGTTTCGAGGTCCACATCCGGTAGCAGATTGa                                          <  1:5478364/93‑1 (MQ=255)

                                               gAACCGCCAGTGTGTTTTTCAGGCTTTGATTCGGATGTGCGTTGCGCTGCTCATTCAGGAAGGTTTCGAGGTCCACATCCGGTAGCAGATTGa                                          >  1:4001675/1‑93 (MQ=255)

                                               gAACCGCCAGTGTGTTTTTCAGGCTTTGATTCGGATGTGCGTTGCGCTGCTCATTCAGGAAGGTTTCGAGGTCCACATCCGGTAGCAGATTGa                                          >  2:734099/1‑93 (MQ=255)

                                                aaCCGCCAGTGTGTTTTTCAGGCTTTGATTCGGATGTGCGTTGCGCTGCTCATTCAGGAAGGTTTCGAGGTCCACATCCGGTAGCAGATTGAt                                         <  1:6473553/93‑1 (MQ=255)

                                                aaCCGCCAGTGTGTTTTTCAGGCTTTGATTCGGATGTGCGTTGCGCTGCTCATTCAGGAAGGTTTCGAGGTCCACATCCGGTAGCAGATTGAt                                         <  1:2213957/93‑1 (MQ=255)

                                                 aCCGCCAGTGTGTTTTTCAGGCTTTGATTCGGATGTGCGTTGCGCTGCTCATTCAGGAAGGTTTCGAGGTCCACATCCGGTAGCAGATTGATg                                        >  2:4684382/1‑93 (MQ=255)

                                                  ccGCCAGTGTGTTTTTCAGGCTTTGATTCGGATGTGCGTTGCGCTGCTCATTCAGGAAGGTTTCGAGGTCCACATCCGGTAGCAGATTGATGc                                       <  2:3402989/93‑1 (MQ=255)

                                                   cGCCAGTGTGTTTTTCAGGCTTTGATTCGGATGTGCGTTGCGCTGCTCATTCAGGAAGGTTTCGAGGTCCACATCCGGTAGCAGATTGATGCt                                      <  2:1763510/93‑1 (MQ=255)

                                                   cGCCAGTGTGTTTTTCAGGCTTTGATTCGGATGTGCGTTGCGCTGCTCATTCAGGAAGGTTTCGAGGTCCACATCCGGTAGCAGATTGATGCt                                      <  1:2683245/93‑1 (MQ=255)

                                                   cGCCAGTGTGTTTTTCAGGCTTTGATTCGGATGTGCGTTGCGCTGCTCATTCAGGAAGGTTTCGAGGTCCACATCCGGTAGCAGATTGATGCt                                      <  2:5911829/93‑1 (MQ=255)

                                                    gCCAGTGTGTTTTTCAGGCTTTGATTCGGATGTGCGTTGCGCTGCTCATTCAGGAAGGTTTCGAGGTCCACATCCGGTAGCAGATTGATGCTg                                     >  2:2040236/1‑93 (MQ=255)

                                                    gCCAGTGTGTTTTTCAGGCTTTGATTCGGATGTGCGTTGCGCTGCTCATTCAGGAAGGTTTCGAGGTCCACATCCGGTAGCAGATTGATGCTg                                     <  1:5931879/93‑1 (MQ=255)

                                                     ccAGTGTGTTTTTCAGGCTTTGATTCGGATGTGCGTTGCGCTGCTCATTCAGGAAGGTTTCGAGGTCCACATCCGGTAGCAGATTGATGCTGa                                    <  1:5261450/93‑1 (MQ=255)

                                                     ccAGTGTGTTTTTCAGGCCTTGATTCGGATGTGCGTTGCGCTGCTCATTCAGGAAGGTTTGGAGGTCCACATCCGGTAGCAGATTGATGCTGa                                    >  1:5660659/1‑93 (MQ=255)

                                                        gtgtgtTTTTCAGGCTTTGATTCGGATGTGCGTTGCGCTGCTCATTCAGGAAGGTTTCGAGGTCCACATCCGGTAGCAGATTGATGCTGACaa                                 <  2:2936867/93‑1 (MQ=255)

                                                         tgtgtTTTTCAGGCTTTGATTCGGATGTGCGTTGCGCTGCTCATTCAGGAAGGTTTCGAGGTCCACATCCGGTAGCAGATTGATGCTGACaaa                                <  1:772388/93‑1 (MQ=255)

                                                         tgtgtTTTTCAGGCTTTGATTCGGATGTGCGTTGCGCTGCTCATTCAGGAAGGTTTCGAGGTCCACATCCGGTAGCAGATTGATGCTGACaaa                                >  1:309360/1‑93 (MQ=255)

                                                            gtTTTTCAGGCTTTGATTCGGATGTGCGTTGCGCTGCTCATTCAGGAAGGTTTCGAGGTCCACATCCGGTAGCAGATTGATGCTGACAAATTc                             >  1:3535912/1‑93 (MQ=255)

                                                             tttttCAGGCTTTGATTCGGATGTGCGTTGCGCTGCTCATTCAGGAAGGTTTCGAGGTCCACATCCGGTAGCAGATTGATGCTGACAAATTcc                            >  1:5574404/1‑93 (MQ=255)

                                                                ttCAGGCTTTGATTCGGATGTGCGTTGCGCTGCTCATTCAGGAAGGTTTCGAGGTCCACATCCGGTAGCAGATTGATGCTGACAAATTccccc                         <  2:4338579/93‑1 (MQ=255)

                                                                ttCAGGCTTTGATTCGGATGTGCGTTGCGCTGCTCATTCAGGAAGGTTTCGAGGTCCACATCCGGTAGCAGATTGATGCTGACAAATTccccc                         <  1:1671944/93‑1 (MQ=255)

                                                                 tCAGGCTTTGATTCGGATGTGCGTTGCGCTGCTCATTCAGGAAGGTTTCGAGGTCCACATCCGGTAGCAGATTGATGCTGACAAATTCCCCCg                        <  2:4595919/93‑1 (MQ=255)

                                                                  cAGGCTTTGATTCGGATGTGCGTTGCGCTGCTCATTCAGGAAGGTTTCGAGGTCCACATCCGGTAGCAGATTGATGCTGACAAATTCCCCCgg                       <  2:4803939/93‑1 (MQ=255)

                                                                   aGGCTTTGATTCGGATGTGCGTTGCGCTGCTCATTCAGGAAGGTTTCGAGGTCCACATCCGGTAGCAGATTGATGCTGACAAATTCCCCCGGt                      >  2:5582227/1‑93 (MQ=255)

                                                                   aGGCTTTGATTCGGATGTGCGTTGCGCTGCTCATTCAGGAAGGTTTCGAGGTCCACATCCGGTAGCAGATTGATGCTGACAAATTCCCCCGGt                      <  1:3958836/93‑1 (MQ=255)

                                                                   aGGCTTTGATTCGGATGTGCGTTGCGCTGCTCATTCAGGAAGGTTTCGAGGTCCACATCCGGTAGCAGATTGATGCTGACAAATTCCCCCGGt                      <  2:1561321/93‑1 (MQ=255)

                                                                   aGGCTTTGATTCGGATGTGCGTTGCGCTGCTCATTCAGGAAGGTTTCGAGGTCCACATCCGGTAGCAGATTGATGCTGACAAATTCCCCCGGt                      >  1:4879270/1‑93 (MQ=255)

                                                                    ggCTTTGATTCGGATGTGCGTTGCGCTGCTCATTCAGGAAGGTTTCGAGGTCCACATCCGGTAGCAGATTGATGCTGACAAATTCCCCCGGtt                     <  1:1285176/93‑1 (MQ=255)

                                                                    ggCTTTGATTCGGATGTGCGTTGCGCTGCTCATTCAGGAAGGTTTCGAGGTCCACATCCGGTAGCAGATTGATGCTGACAAATTCCCCCGGtt                     >  2:2662600/1‑93 (MQ=255)

                                                                      cTTTGATTCGGATGTGCGTTGCGCTGCTCATTCAGGAAGGTTTCGAGGTCCACATCCGGTAGCAGATTGATGCTGACAAATTCCCCCGGTTGc                   >  1:5810266/1‑93 (MQ=255)

                                                                      cTTTGATTCGGATGTGCGTTGCGCTGCTCATTCAGGAAGGTTTCGAGGTCCACATCCGGTAGCAGATTGATGCTGACAAATTCCCCCGGTTGc                   >  2:6528510/1‑93 (MQ=255)

                                                                      cTTTGATTCGGATGTGCGTTGCGCTGCTCATTCAGGAAGGTTTCGAGGTCCACATCCGGTAGCAGATTGATGCTGACAAATTCCCCCGGTTGc                   <  1:6588702/93‑1 (MQ=255)

                                                                       tttGATTCGGATGTGCGTTGCGCTGCTCATTCAGGAAGGTTTCGAGGTCCACATCCGGTAGCAGATTGATGCTGACAAATTCCCCCGGTTGcc                  >  1:1458648/1‑93 (MQ=255)

                                                                       tttGATTCGGATGTGCGTTGCGCTGCTCATTCAGGAAGGTTTCGAGGTCCACATCCGGTAGCAGATTGATGCTGACAAATTCCCCCGGTTGcc                  >  1:4524832/1‑93 (MQ=255)

                                                                       tttGATTCGGATGTGCGTTGCGCTGCTCATTCAGGAAGGTTTCGAGGTCCACATCCGGTAGCAGATTGATGCTGACAAATTCCCCCGGTTGcc                  <  2:691900/93‑1 (MQ=255)

                                                                       tttGATTCGGATGTGCGTTGCGCTGCTCATTCAGGAAGGTTTCGAGGTCCACATCCGGTAGCAGATTGATGCTGACAAATTCCCCCGGTTGcc                  <  1:2947073/93‑1 (MQ=255)

                                                                          gATTCGGATGTGCGTTGCGCTGCTCATTCAGGAAGGTTTCGAGGTCCACATCCGGTAGCAGATTGATGCTGACAAATTCCCCCGGTTGCCAGt               >  1:6352146/1‑93 (MQ=255)

                                                                           aTTCGGATGTGCGTTGCGCTGCTCATTCAGGAAGGTTTCGAGGTCCACATCCGGTAGCAGATTGATGCTGACAAATTCCCCCGGTTGCCAGTa              <  1:1071439/93‑1 (MQ=255)

                                                                           aTTCGGATGTGCGTTGCGCTGCTCATTCAGGAAGGTTTCGAGGTCCACATCCGGTAGCAGATTGATGCTGACAAATTCCCCCGGTTGCCAGTa              >  1:1622820/1‑93 (MQ=255)

                                                                            ttCGGATGTGCGTTGCGCTGCTCATTCAGGAAGGTTTCGAGGTCCACATCCGGTAGCAGATTGATGCTGACAAATTCCCCCGGTTGCCAGTAg             >  2:5148218/1‑93 (MQ=255)

                                                                             tCGGATGTGCGTTGCGCTGCTCATTCAGGAAGGTTTCGAGGTCCACATCCGGTAGCAGATTGATGCTGACAAATTCCCCCGGTTGCCAGTAGc            <  2:123974/93‑1 (MQ=255)

                                                                              cGGATGTGCGTTGCGCTGCTCATTCAGGAAGGTTTCGAGGTCCACATCCGGTAGCAGATTGATGCTGACAAATTCCCCCGGTTGCCAGTAGCt           <  2:4420349/93‑1 (MQ=255)

                                                                              cGGATGTGCGTTGCGCTGCTCATTCAGGAAGGTTTCGAGGTCCACATCCGGTAGCAGATTGATGCTGACAAATTCCCCCGGTTGCCAGTAGCt           >  1:6110986/1‑93 (MQ=255)

                                                                               ggATGTGCGTTGCGCTGCTCATTCAGGAAGGTTTCGAGGTCCACATCCGGTAGCAGATTGATGCTGACAAATTCCCCCGGTTGCCAGTAGCtt          <  2:1670570/93‑1 (MQ=255)

                                                                                 aTGTGCGTTGCGCTGCTCATTCAGGAAGGTTTCGAGGTCCACATCCGGTAGCAGATTGATGCTGATAAATTCCCCCGGTTGCCAGTAGCTTGa        >  1:2407761/1‑93 (MQ=255)

                                                                                  tgtgCGTTGCGCTGCTCATTCAGGAAGGTTTCGAGGTCCACATCCGGTAGCCGATTGATGCTGACAAATTCCCCCGGTTGCCAGTAGCTTGaa       >  1:1751316/1‑93 (MQ=255)

                                                                                  tgtgCGTTGCGCTGCTCATTCAGGAAGGTTTCGAGGTCCACATCCGGTAGCAGATTGATGCTGACAAATTCCCCCGGTTGCCAGTAGCTTGaa       >  2:3939061/1‑93 (MQ=255)

                                                                                   gtgCGTTGCGCTGCTCATTCAGGAAGGTTTCGAGGTCCACATCCGGTAGCAGATTGATGCTGACAAATTCCCCCGGTTGCCAGTAGCTTGaaa      >  2:889541/1‑93 (MQ=255)

                                                                                    tgCGTTGCGCTGCTCATTCAGGAAGGTTTCGAGGTCCACATCCGGTAGCAGATTGATGCTGACAAATTCCCCCGGTTGCCAGTAGCTTGAAAt     >  2:4175636/1‑93 (MQ=255)

                                                                                      cGTTGCGCTGCTCATTCAGGAAGGTTTCGAGGTCCACATCCGGTAGCAGATTGATGCTGACAAATTCCCCCGGTTGCCAGTAGCTTGAAATCt   >  2:3715552/1‑93 (MQ=255)

GATTTGCCCGAGTTGCTGTAAGCGTTCAACCAACCGCTTCGGTAGATGAACCGCCAGTGTGTTTTTCAGGCTTTGATTCGGATGTGCGTTACGCTGCTCATTCAGGAAGGTTTCGAGGTCCACATCCGGTAGCAGATTGATGCTGACAAATTCCCCCGGTTGCCAGTAGCTTGAAATCTG  >  NC_000913/3634138‑3634317

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 20 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`