BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,649,511	G→A	E197E (GAG→GAA)	yhiS →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,649,511	0	G	A	99.6%	929.3 / ‑5.3	249	E197E (GAG→GAA)	yhiS	hypothetical protein
Reads supporting (aligned to +/- strand): ref base G (0/1); new base A (129/119); total (129/120)
Fisher's exact test for biased strand distribution p-value = 4.82e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.47e-01

Only 100 of 253 total aligned reads displayed.

TAATTTTATTGCTGAACAACGAGCTGACTTACCAACTAATATAAAAGATATTCAAAGCCTGTATCAAAAAATGACGAAGTTATATATTGAGCACAGTGAAAATAAAAACAGGATGAAAGTCTTTGCCGGAACCAACTTTATTGATTTCAATATGACAGGACAGAACCTGTCCGGGTTCGT  >  NC_000913/3649421‑3649600

 aaTTTTATTGCTGAACAACGAGCTGACTTACCAACTAATATAAAAGATATTCAAAGCCTGTATCAAAAAATGACGAAGTTATATATTGAacac                                                                                        >  2:5840322/1‑93 (MQ=255)

    tttATTGCTGAACAACGAGCTGACTTACCAACTAATATAAAAGATATTCAAAGCCTGTATCAAAAAATGACGAAGTTATATATTGAACACAGt                                                                                     <  2:5987940/93‑1 (MQ=255)

     ttATTGCTGAACAACGAGCTGACTTACCAACTAATATAAAAGATATTCAAAGCCTGTATCAAAAAATGACGAAGTTATATATTGAACACAGTg                                                                                    <  1:966378/93‑1 (MQ=255)

     ttATTGCTGAACAACGAGCTGACTTACCAACTAATATAAAAGATATTCAAAGCCTGTATCAAAAAATGACGAAGTTATATATTGAACACAGTg                                                                                    <  1:4512516/93‑1 (MQ=255)

       aTTGCTGAACAACGAGCTGACTTACCAACTAATATAAAAGATATTCAAAGCCTGTATCAAAAAATGACGAAGTTATATATTGAACACAGTGaa                                                                                  >  1:4815447/1‑93 (MQ=255)

       aTTGCTGAACAACGAGCTGACTTACCAACTAATATAAAAGATATTCAAAGCCTGTATCAAAAAATGACGAAGTTATATATTGAACACAGTGaa                                                                                  <  2:5172431/93‑1 (MQ=255)

        ttGCTGAACAACGAGCTGACTTACCAACTAATATAAAAGATATTCAAAGCCTGTATCAAAAAATGACGAAGTTATATATTGAACACAGTGaaa                                                                                 <  1:2109366/93‑1 (MQ=255)

        ttGCTGAACAACGAGCTGACTTACCAACTAATATAAAAGATATTCAAAGCCTGTATCAAAAAATGACGAAGTTATATATTGAACACAGTGaaa                                                                                 >  2:3990976/1‑93 (MQ=255)

         tGCTGAACAACGAGCTGACTTACCAACTAATATAAAAGATATTCAAAGCCTGTATCAAAAAATGACGAAGTTATATATTGAACACAGTGaaaa                                                                                >  2:5597786/1‑93 (MQ=255)

         tGCTGAACAACGAGCTGACTTACCAACTAATATAAAAGATATTCAAAGCCTGTATCAAAAAATGACGAAGTTATATATTGAACACAGTGaaaa                                                                                >  1:6626570/1‑93 (MQ=255)

           cTGAACAACGAGCTGACTTACCAACTAATATAAAAGATATTCAAAGCCTGTATCAAAAAATGACGAAGTTATATATTGAACACAGTGAAAATa                                                                              <  2:5760639/93‑1 (MQ=255)

             gAACAACGAGCTGACTTACCAACTAATATAAAAGATATTCAAAGCCTGTATCAAAAAATGACGAAGTTATATATTGAACACAGTGAAAATaaa                                                                            >  2:5871460/1‑93 (MQ=255)

               acaacGAGCTGACTTACCAACTAATATAAAAGATATTCAAAGCCTGTATCAAAAAATGACGAAGTTATATATTGAACACAGTGAAAATaaaaa                                                                          <  2:2814159/93‑1 (MQ=255)

                 aacGAGCTGACTTACCAACTAATATAAAAGATATTCAAAGCCTGTATCAAAAAATGACGAAGTTATATATTGAACACAGTGAAAATAAAAACa                                                                        <  1:4657521/93‑1 (MQ=255)

                  acGAGCTGACTTACCAACTAATATAAAAGATATTCAAAGCCTGTATCAAAAAATGACGAAGTTATATATTGAACACAGTGAAAATAAAAACAg                                                                       <  2:1418507/93‑1 (MQ=255)

                  acGAGCTGACTTACCAACTAATATAAAAGATATTCAAAGCCTGTATCAAAAAATGACGAAGTTATATATTGAACACAGTGAAAATAAAAACAg                                                                       >  1:6492767/1‑93 (MQ=255)

                   cGAGCTGACTTACCAACTAATATAAAAGATATTCAAAGCCTGTATCAAAAAATGACGAAGTTATATATTGAACACAGTGAAAATAAAAACAgg                                                                      <  2:6257018/93‑1 (MQ=255)

                    gAGCTGACTTACCAACTAATATAAAAGATATTCAAAGCCTGTATCAAAAAATGACGAAGTTATATATTGAACACAGTGAAAATAAAAACAGGa                                                                     <  1:112203/93‑1 (MQ=255)

                    gAGCTGACTTACCAACTAATATAAAAGATATTCAAAGCCTGTATCAAAAAATGACGAAGTTATATATTGAACACAGTGAAAATAAAAACAGGa                                                                     <  2:2215025/93‑1 (MQ=255)

                     aGCTGACTTACCAACTAATATAAAAGATATTCAAAGCCTGTATCAAAAAATGACGAAGTTATATATTGAACACAGTGAAAATAAAAACAGGAt                                                                    <  1:3195587/93‑1 (MQ=255)

                     aGCTGACTTACCAACTAATATAAAAGATATTCAAAGCCTGTATCAAAAAATGACGAAGTTATATATTGAACACAGTGAAAATAAAAACAGGAt                                                                    <  1:4574047/93‑1 (MQ=255)

                     aGCTGACTTACCAACTAATATAAAAGATATTCAAAGCCTGTATCAAAAAATGACGAAGTTATATATTGAACACAGTGAAAATAAAAACAGGAt                                                                    <  2:5711431/93‑1 (MQ=255)

                        tGACTTACCAACTAATATAAAAGATATTCAAAGCCTGTATCAAAAAATGACGAAGTTATATATTGAACACAGTGAAAATAAAAACAGGATGaa                                                                 >  1:1638182/1‑93 (MQ=255)

                         gACTTACCAACTAATATAAAAGATATTCAAAGCCTGTATCAAAAAATGACGAAGTTATATATTGAACACAGTGAAAATAAAAACAGGATGaaa                                                                <  1:2766372/93‑1 (MQ=255)

                          aCTTACCAACTAATATAAAAGATATTCAAAGCCTGTATCAAAAAATGACGAAGTTATATATTGAACACAGTGAAAATAAAAACAGGATGAAAg                                                               <  2:1694201/93‑1 (MQ=255)

                           cTTACCAACTAATATAAAAGATATTCAAAGCCTGTATCAAAAAATGACGAAGTTATATATTGAACACAGTGAAAATAAAAACAGGATGAAAGt                                                              >  1:4908626/1‑93 (MQ=255)

                            ttACCAACTAATATAAAAGATATTCAAAGCCTGTATCAAAAAATGACGAAGTTATATATTGAACACAGTGAAAATAAAAACAGGATGAAAGTc                                                             >  2:5059329/1‑93 (MQ=255)

                             tACCAACAAATATAAAAGATATTCAAAGCCTGTATCAAAAAATGACGAAGTTATATATTGAACACAGTGAAAATAAAAACAGGATGAAAGTCt                                                            >  1:6608674/1‑93 (MQ=255)

                              aCCAACTAATATAAAAGATATTCAAAGCCTGTATCAAAAAATGACGAAGTTATATATTGAACACAGTGAAAATAAAAACAGGATGAAAGTCtt                                                           <  1:5180597/93‑1 (MQ=255)

                               ccAACTAATATAAAAGATATTCAAAGCCTGTATCAAAAAATGACGAAGTTATATATTGAACACAGTGAAAATAAAAACAGGATGAAAGTCttt                                                          <  2:2497985/93‑1 (MQ=255)

                               ccAACTAATATAAAAGATATTCAAAGCCTGTATCAAAAAATGACGAAGTTATATATTGAACACAGTGAAAATAAAAACAGGATGAAAGTCttt                                                          >  2:1161866/1‑93 (MQ=255)

                                cAACTAATATAAAAGATATTCAAAGCCTGTATCAAAAAATGACGAAGTTATATATTGAACACAGTGAAAATAAAAACAGGATGAAAGTCTTTg                                                         >  1:6371678/1‑93 (MQ=255)

                                 aaCTAATATAAAAGATATTCAAAGCCTGTATCAAAAAATGACGAAGTTATATATTGAACACAGTGAAAATAAAAACAGGATGAAAGTCTTTGc                                                        >  2:3659564/1‑93 (MQ=255)

                                 aaCTAATATAAAAGATATTCAAAGCCTGTATCAAAAAATGACGAAGTTATATATTGAACACAGTGAAAATAAAAACAGGATGAAAGTCTTTGc                                                        >  1:6329592/1‑93 (MQ=255)

                                   cTAATATAAAAGATATTCATAGCCTGTATCAAAAAATGACGAAGTTATATATTGAACACAGTGAAAATAAAAACAGGATGAAAGTCTTTGCCg                                                      >  1:6732641/1‑93 (MQ=255)

                                   cTAATATAAAAGATATTCAAAGCCTGTATCAAAAAATGACGAAGTTATATATTGAACACAGTGAAAATAAAAACAGGATGAAAGTCTTTGCCg                                                      <  1:4287113/93‑1 (MQ=255)

                                      atatAAAAGATATTCAAAGCCTGTATCAAAAAATGACGAAGTTATATATTGAACACAGTGAAAATAAAAACAGGATGAAAGTCTTTGCCGGaa                                                   >  2:593168/1‑93 (MQ=255)

                                        ataAAAGATATTCAAAGCCTGTATCAAAAAATGACGAAGTTATATATTGAACACAGTGAAAATAAAAACAGGATGAAAGTCTTTGCCGGAAcc                                                 <  1:4422903/93‑1 (MQ=255)

                                         taAAAGATATTCAAAGCCTGTATCAAAAAATGACGAAGTTATATATTGAACACAGTGAAAATAAAAACAGGATGAAAGTCTTTGCCGGAACCa                                                <  2:4510767/93‑1 (MQ=255)

                                           aaaGATATTCAAAGCCTGTATCAAAAAATGACGAAGTTATATATTGAACACAGTGAAAATAAAAACAGGATGAAAGTCTTTGCCGGAACCAAc                                              >  2:3877369/1‑93 (MQ=255)

                                            aaGATATTCAAAGCCTGTATCAAAAAATGACGAAGTTATATATTGAACACAGTGAAAATAAAAACAGGATGAAAGTCTTTGCCGGAACCAACt                                             <  2:3917321/93‑1 (MQ=255)

                                             aGATATTCAAAGCCTGTATCAAAAAATGACGAAGTTATATATTGAACACAGTGAAAATAAAAACAGGATGAAAGTCTTTGCCGGAACCAACtt                                            <  2:3454624/93‑1 (MQ=255)

                                             aGATATTCAAAGCCTGTATCAAAAAATGACGAAGTTATATATTGAACACAGTGAAAATAAAAACAGGATGAAAGTCTTTGCCGGAACCAACtt                                            <  2:3058256/93‑1 (MQ=255)

                                             aGATATTCAAAGCCTGTATCAAAAAATGACGAAGTTATATATTGAACACAGTGAAAATAAAAACAGGATGAAAGTCTTTGCCGGAACCAACtt                                            >  1:37168/1‑93 (MQ=255)

                                              gATATTCAAAGCCTGTATCAAAAAATGACGAAGTTATATATTGAACACAGTGAAAATAAAAACAGGATGAAAGTCTTTGCCGGCACCAACttt                                           >  1:5421646/1‑93 (MQ=255)

                                              gATATTCAAAGCCTGTATCAAAAAATGACGAAGTTATATATTGAACACAGTGAAAATAAAAACAGGATGAAAGTCTTTGCCGGAACCAACttt                                           >  2:5316003/1‑93 (MQ=255)

                                               atatTCAAAGCCTGTATCAAAAAATGACGAAGTTATATATTGAACACAGTGAAAATAAAAACAGGATGAAAGTCTTTGCCGGAACCAACTTTa                                          <  1:4589712/93‑1 (MQ=255)

                                                tatTCAAAGCCTGTATCAAAAAATGACGAAGTTATATATTGAACACAGTGAAAATAAAAACAGGATGAAAGTCTTTGCCGGAACCAACTTTAt                                         <  1:1218064/93‑1 (MQ=255)

                                                tatTCAAAGCCTGTATCAAAAAATGACGAAGTTATATATTGAACACAGTGAAAATAAAAACAGGATGAAAGTCTTTGCCGGAACCAACTTTAt                                         <  1:2643417/93‑1 (MQ=255)

                                                tatTCAAAGCCTGTATCAAAAAATGACGAAGTTATATATTGAACACAGTGAAAATAAAAACAGGATGAAAGTCTTTGCCGGAACCAACTTTAt                                         <  2:2113369/93‑1 (MQ=255)

                                                tatTCAAAGCCTGTATCAAAAAATGACGAAGTTATATATTGAACACAGTGAAAATAAAAACAGGATGAAAGTCTTTGCCGGAACCAACTTTAt                                         >  2:4731397/1‑93 (MQ=255)

                                                 atTCAAAGCCTGTATCAAAAAATGACGAAGTTATATATTGAACACAGTGAAAATAAAAACAGGATGAAAGTCTTTGCCGGAACCAACTTTact                                        <  1:6413528/93‑3 (MQ=255)

                                                 atTCAAAGCCTGTATCAAAAAATGACGAAGTTATATATTGAACACAGTGAAAATAAAAACAGGATGAAAGTCTTTGCCGGAACCAACTTTAtt                                        >  1:3120799/1‑93 (MQ=255)

                                                   tCAAAGCCTGTATCAAAAAATGACGAAGTTATATATTGAACACAGTGCAAATAAAAACAGGATGAAAGTCTTTGCCGGAACCAACTTTATTGa                                      >  1:1817462/1‑93 (MQ=255)

                                                   tCAAAGCCTGTATCAAAAAATGACGAAGTTATATATTGAACACAGTGAAAATAAAAACAGGATGAAAGTCTTTGCCGGAACCAACTTTATTGa                                      >  2:4102639/1‑93 (MQ=255)

                                                    cAAAGCCTGTATCAAAAAATGACGAAGTTATATATTGAACACAGTGAAAATAAAAACAGGATGAAAGTCTTTGCCGGAACCAACTTTATTGAt                                     >  1:5903881/1‑93 (MQ=255)

                                                    cAAAGCCTGTATCAAAAAATGACGAAGTTATATATTGAACACAGTGAAAATAAAAACAGGATGAAAGTCTTTGCCGGAACCAACTTTATTGAt                                     <  1:4235449/93‑1 (MQ=255)

                                                    cAAAGCCTGTATCAAAAAATGACGAAGTTATATATTGAACACAGTGAAAATAAAAACAGGATGAAAGTCTTTGCCGGAACCAACTTTATTGAt                                     <  1:3915754/93‑1 (MQ=255)

                                                    cAAAGCCTGTATCAAAAAATGACGAAGTTATATATTGAACACAGTGAAAATAAAAACAGGATGAAAGTCTTTGCCGGAACCAACTTTATTGAt                                     <  1:1006896/93‑1 (MQ=255)

                                                     aaaGCCTGTATCAAAAAATGACGAAGTTATATATTGAACACAGTGAAAATAAAAACAGGATGAAAGTCTTTGCCGGAACCAACTTTATTGAtt                                    <  2:1395198/93‑1 (MQ=255)

                                                     aaaGCCTGTATCAAAAAATGACGAAGTTATATATTGAACACAGTGAAAATAAAAACAGGATGAAAGTCTTTGCCGGAACCAACTTTATTGAtt                                    <  1:1366218/93‑1 (MQ=255)

                                                      aaGCCTGTATCAAAAAATGACGAAGTTATATATTGAACACAGTGAAAATAAAAACAGGATGAAAGTCTTTGCCGGAACCAACTTTATTGAttt                                   >  2:6557293/1‑93 (MQ=255)

                                                       aGCCTGTATCAAAAAATGACGAAGTTATATATTGAACACAGTGAAAATAAAAACAGGATGAAAGTCTTTGCCGGAACCAACTTTATTGATTTc                                  >  1:728679/1‑93 (MQ=255)

                                                       aGCCTGTATCAAAAAATGACGAAGTTATATATTGAACACAGTGAAAATAAAAACAGGATGAAAGTCTTTGCCGGAACCAACTTTATTGATTTc                                  >  2:2716044/1‑93 (MQ=255)

                                                        gCCTGTATCAAAAAATGACGAAGTTATATATTGAACACAGTGAAAATAAAAACAGGATGAAAGTCTTTGCCGGAACCAACTTTATTGATTTCa                                 >  1:2153152/1‑93 (MQ=255)

                                                        gCCTGTATCAAAAAATGACGAAGTTATATATTGAACACAGTGAAAATAAAAACAGGATGAAAGTCTTTGCCGGAACCAACTTTATTGATTTCa                                 >  2:6704851/1‑93 (MQ=255)

                                                          cTGTATCAAAAAATGACGAAGTTATATATTGAACACAGTGAAAATAAAAACAGGATGAAAGTCTTTGCCGGAACCAACTTTATTGATTTCAat                               <  2:5338123/93‑1 (MQ=255)

                                                          cTGTATCAAAAAATGACGAAGTTATATATTGAACACAGTGAAAATAAAAACAGGATGAAAGTCTTTGCCGGAACCAACTTTATTGATTTCAat                               >  2:497188/1‑93 (MQ=255)

                                                           tGTATCAAAAAATGACGAAGTTATATATTGAACACAGTGAAAATAAAAACAGGATGAAAGTCTTTGCCGGAACCAACTTTATTGATTTCAata                              <  1:4211447/93‑1 (MQ=255)

                                                           tGTATCAAAAAATGACGAAGTTATATATTGAACACAGTGAAAATAAAAACAGGATGAAAGTCTTTGCCGGAACCAACTTTATTGATTTCAata                              >  2:1100272/1‑93 (MQ=255)

                                                            gTATCAAAAAATGACGAAGTTATATATTGAACACAGTGAAAATAAAAACAGGATGAAAGTCTTTGCCGGAACCAACTTTATTGATTTCAatat                             <  2:5206675/93‑1 (MQ=255)

                                                             tATCAAAAAATGACGAAGTTATATATTGAACACAGTGAAAATAAAAACAGGATGAAAGTCTTTGCCGGAACCAACTTTATTGATTTCAATATg                            >  1:1984163/1‑93 (MQ=255)

                                                              aTCAAAAAATGACGAAGTTATATATTGAACACAGTGAAAATAAAAACAGGATGAAAGTCTTTGCCGGAACCAACTTTATTGATTTCAATATga                           >  1:5803537/1‑93 (MQ=255)

                                                                cAAAAAATGACGAAGTTATATATTGAACACAGTGAAAATAAAAACAGGATGAAAGTCTTTGCCGGAACCAACTTTATTGATTTCAATATgaca                         <  2:6097350/93‑1 (MQ=255)

                                                                cAAAAAATGACGAAGTTATATATTGAACACAGTGAAAATAAAAACAGGATGAAAGTCTTTGCCGGAACCAACTTTATTGATTTCAATATgaca                         <  1:6080443/93‑1 (MQ=255)

                                                                 aaaaaaTGACGAAGTTATATATTGAACACAGTGAAAATAAAAACAGGATGAAAGTCTTTGCCGGAACCAACTTTATTGATTTCAATATgacag                        <  2:5504872/93‑1 (MQ=255)

                                                                 aaaaaaTGACGAAGTTATATATTGAACACAGTGAAAATAAAAACAGGATGAAAGTCTTTGCCGGAACCAACTTTATTGATTTCAATATgacag                        <  2:4634175/93‑1 (MQ=255)

                                                                 aaaaaaTGACGAAGTTATATATTGAACACAGTGAAAATAAAAACAGGATGAAAGTCTTTGCCGGAACCAACTTTATTGATTTCAATATgacag                        <  2:3949746/93‑1 (MQ=255)

                                                                   aaaaTGACGAAGTTATATATTGAACACAGTGAAAATAAAAACAGGATGAAAGTCTTTGCCGGAACCAACTTTATTGATTTCAATATgacagga                      >  2:440884/1‑93 (MQ=255)

                                                                   aaaaTGACGAAGTTATATATTGAACACAGTGAAAATAAAAACAGGATGAAAGTCTTTGCCGGAACCAACTTTATTGATTTCAATATgacagga                      <  2:2547856/93‑1 (MQ=255)

                                                                      aTGACGAAGTTATATATTGAACACAGTGAAAATAAAAACAGGATGAAAGTCTTTGCCGGAACCAACTTTATTGATTTCAATATgacaggacag                   <  2:4314525/93‑1 (MQ=255)

                                                                           gAAGTTATATATTGAACACAGTGAAAATAAAAACAGGATGAAAGTCTTTGCCGGAACCAACTTTATTGATTTCAATATGACAGGACAGAACCt              >  1:617153/1‑93 (MQ=255)

                                                                            aaGTTATATATTGAACACAGTGAAAATAAAAACAGGATGAAAGTCTTTGCCGGAACCAACTTTATTGATTTCAATATGACAGGACAGAACCTg             >  1:1566838/1‑93 (MQ=255)

                                                                             aGTTATATATTGAACACAGTGAAAATAAAAACAGGATGAAAGTCTTTGCCGGAACCAACTTTATTGATTTCAATATGACAGGACAGAACCTGt            >  2:1928585/1‑93 (MQ=255)

                                                                              gTTATATATTGAACACAGTGAAAATAAAAACAGGATGAAAGTCTTTGCCGGAACCAACTTTATTGATTTCAATATGACAGGACAGAACCTGTc           >  2:3820757/1‑93 (MQ=255)

                                                                               ttATATATTGAACACAGTGAAAATAAAAACAGGATGAAAGTCTTTGCCGGAACCAACTTTATTGATTTCAATATGACAGGACAGAACCTGTcc          <  2:3124728/93‑1 (MQ=255)

                                                                               ttATATATTGAACACAGTGAAAATAAAAACAGGATGAAAGTCTTTGCCGGAACCAACTTTATTGATTTCAATATGACAGGACAGAACCTGTcc          >  2:4589105/1‑93 (MQ=255)

                                                                               ttATATATTGAACACAGTGAAAATAAAAACAGGATGAAAGTCTTTGCCGGAACCAACTTTATTGATTTCAATATGACAGGACAGAACCTGTcc          >  2:1065681/1‑93 (MQ=255)

                                                                                tatataTTGAACACAGTGAAAATAAAAACAGGATGAAAGTCTTTGCCGGAACCAACTTTATTGATTTCAATATGACAGGACAGAACCTGTCCg         >  2:1732661/1‑93 (MQ=255)

                                                                                 atatatTGAACACAGTGAAAATAAAAACAGGATGAAAGTCTTTGCCGGAACCAACTTTATTGATTTCAATATGACAGGACAGAACCTGTCCgg        <  1:1200619/93‑1 (MQ=255)

                                                                                 atatatTGAACACAGTGAAAATAAAAACAGGATGAAAGTCTTTGCCGGAACCAACTTTATTGATTTCAATATGACAGGACAGAACCTGTCCgg        >  1:924495/1‑93 (MQ=255)

                                                                                   atatTGAACACAGTGAAAATAAAAACAGGATGAAAGTCTTTGCCGGAACCAACTTTATTGATTTCAATATGACAGGACAGAACCTGTCCGGgt      <  2:4807196/93‑1 (MQ=255)

                                                                                   atatTGAACACAGTGAAAATAAAAACAGGATGAAAGTCTTTGCCGGAACCAACTTTATTGATTTCAATATGACAGGACAGAACCTGTCCGGgt      <  1:3863095/93‑1 (MQ=255)

                                                                                    tatTGAACACGGTGAAAATAAAAACAGGATGAAAGTCTTTGCCGGAACCAACTTTATTGATTTCAATATGACAGGACAGAACCTGTCCGGgtt     <  2:4080793/93‑1 (MQ=255)

                                                                                    tatTGAACACAGTGAAAATAAAAACAGGATGAAAGTCTTTGCCGGAACCAACTTTATTGATTTCAATATGACAGGACAGAACCTGTCCGGgtt     <  1:5383622/93‑1 (MQ=255)

                                                                                    tatTGAACACAGTGAAAATAAAAACAGGATGAAAGTCTTTGCCGGAACCAACTTTATTGATTTCAATATGACAGGACAGAACCTGTCCGGgtt     <  2:1835753/93‑1 (MQ=255)

                                                                                     atTGAACACAGTGAAAATAAAAACAGGATGAAAGTCTTTGCCGGAACCAACTTTATTGATTTCAATATGACAGGACAGAACCTGTCCGGgttc    >  1:5270014/1‑93 (MQ=255)

                                                                                     atTGAACACAGTGAAAATAAAAACAGGATGAAAGTCTTTGCCGGAACCAACTTTATTGATTTCAATATGACAGGACAGAACCTGTCCGGgttc    >  2:896741/1‑93 (MQ=255)

                                                                                       tGAACACAGTGAAAATAAAAACAGGATGAAAGTCTTTGCCGGAACCAACTTTATTGATTTCAATATGACAGGACAGAACCTGTCCGGgttcgt  >  1:1480450/1‑93 (MQ=255)

                                                                                       tGAACACAGTGAAAATAAAAACAGGATGAAAGTCTTTGCCGGAACCAACTTTATTGATTTCAATATGACAGGACAGAACCTGTCCGGgttcgt  <  1:571922/93‑1 (MQ=255)

TAATTTTATTGCTGAACAACGAGCTGACTTACCAACTAATATAAAAGATATTCAAAGCCTGTATCAAAAAATGACGAAGTTATATATTGAGCACAGTGAAAATAAAAACAGGATGAAAGTCTTTGCCGGAACCAACTTTATTGATTTCAATATGACAGGACAGAACCTGTCCGGGTTCGT  >  NC_000913/3649421‑3649600

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 26 ≤` `ATCG/ATCG` `< 31 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`