BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,683,091	A→G	V42A (GTC→GCC)	yhjK ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,683,091	0	A	G	99.6%	805.2 / ‑4.8	248	V42A (GTC→GCC)	yhjK	hypothetical protein
Reads supporting (aligned to +/- strand): ref base A (1/0); new base G (128/119); total (129/119)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.24e-01

Only 100 of 255 total aligned reads displayed.

GACCACGCCTGCCGGTTTAATGCTGGCAAGAATAGCTTCCGCTTCGGGAATATCGCCTTTCAAAATAGCTGAAGATAAGGGTTCGCGGACAGAGCGAGCAATGCTTTCCAGTTGCGTAGCCGTGTTATAGCGATTCTGCTGGACCAGATGGAACAGCAAAACGGTGCAAAAAATAAAA  >  NC_000913/3683003‑3683180

gACCACGCCTGCCGGTTTAATGCTGGCAAGAATAGCTTCCGCTTCGGGAATATCGCCTTTCAAAATAGCTGAAGATAAGGGTTCGCGGGCaga                                                                                       <  2:4008961/93‑1 (MQ=255)

gACCACGCCTGCCGGTTTAATGCTGGCAAGAATAGCTTCCGCTTCGGGAATATCGCCTTTCAAAATAGCTGAAGATAAGGGTTCGCGGGCaga                                                                                       <  1:4446116/93‑1 (MQ=255)

  ccACGCCTGCCGGTTTAATGCTGGCAAGAATAGCTTCCGCTTCGGGAATATCGCCTTTCAAAATAGCTGAAGATAAGGGTTCGCGGGCAgagc                                                                                     >  2:1307788/1‑93 (MQ=255)

   cACGCCTGCCGGTTTAATGCTGGCAAGAATAGCTTCCGCTTCGGGAATATCGCCTTTCAAAATAGCTGAAGATAAGGGTTCGCGGGCAgagcg                                                                                    >  1:4177021/1‑93 (MQ=255)

    aCGCCTGCCGGTTTAATGCTGGCAAGAATAGCTTCCGCTTCGGGAATATCGCCTTTCAAAATAGCTGAAGATAAGGGTTCGCGGGCAgagcga                                                                                   <  2:4308454/93‑1 (MQ=255)

      gCCTGCCGGTTTAATGCTGGCAAGAATAGCTTCCGCTTCGGGAATATCGCCTTTCAAAATAGCTGAAGATAAGGGTTCGCGGGCAgagcgagc                                                                                 <  2:6583350/93‑1 (MQ=255)

       ccTGCCGGTTTAATGCTGGCAAGAATAGCTTCCGCTTCGGGAATATCGCCTTTCAAAATAGCTGAAGATAAGGGTTCGCGGGCAGAGCGAGCa                                                                                <  2:5930042/93‑1 (MQ=255)

         tGCCGGTTTAATGCTGGCAAGAATAGCTTCCGCTTCGGGAATATCGCCTTTCAAAATAGCTGAAGATAAGGGTTCGCGGGCAGAGCGAGCAAt                                                                              <  2:4944793/93‑1 (MQ=255)

         tGCCGGTTTAATGCTGGCAAGAATAGCTTCCGCTTCGGGAATATCGCCTTTCAAAATAGCTGAAGATAAGGGTTCGCGGGCAGAGCGAGCAAt                                                                              <  2:2718834/93‑1 (MQ=255)

             ggTTTAATGCTGGCAAGAATAGCTTCCGCTTCGGGAATATCGCCTTTCAAAATAGCTGAAGATAAGGGTTCGCGGGCAGAGCGAGCAATGCtt                                                                          >  1:1581433/1‑93 (MQ=255)

              gTTTAATGCTGGCAAGAATAGCTTCCGCTTCGGGAATATCGCCTTTCAAAATAGCTGAAGATAAGGGTTCGCGGGCAGAGCGAGCAATGCttt                                                                         >  2:1904205/1‑93 (MQ=255)

                ttAATGCTGGCAAGAATAGCTTCCGCTTCGGGAATATCGCCTTTCAAAATAGCTGAAGATAAGGGTTCGCGGGCAGAGCGAGCACTGCTTTcc                                                                       <  1:6536119/93‑1 (MQ=255)

                   aTGCTGGCAAGAATAGCTTCCGCTTCGGGAATATCGCCTTTCAAAATAGCTGAAGATAAGGGTTCGCGGGCAGAGCGAGCAATGCTTTCCAGt                                                                    >  2:2168314/1‑93 (MQ=255)

                   aTGCTGACAAGAATAGCTTCCGCTTCGGGAATATCGCCTTTCAAAATAGCTGAAGATAAGGGTTCGCGGGCAGAGCGAGCAATGCTTTCCAGt                                                                    <  2:3826081/93‑1 (MQ=255)

                    tGCTGGCAAGAATAGCTTCCGCTTCGGGAATATCGCCTTTCAAAATAGCTGAAGATAAGGGTTCGCGGGCAGAGCGAGCAATGCTTTCCAGtt                                                                   <  1:4470411/93‑1 (MQ=255)

                    tGCTGGCAAGAATAGCTTCCGCTTCGGGAATATCGCCTTTCAAAATAGCTGAAGATAAGGGTTCGCGGGCAGAGCGAGCAATGCTTTCCAGtt                                                                   >  1:397066/1‑93 (MQ=255)

                    tGCTGGCAAGAATAGCTTCCGCTTCGGGAATATCGCCTTTCAAAATAGCTGAAGATAAGGGTTCGCGGGCAGAGCGAGCAATGCTTTCCAGtt                                                                   <  2:3279022/93‑1 (MQ=255)

                    tGCTGGCAAGAATAGCTTCCGCTTCGGGAATATCGCCTTTCAAAATAGCTGAAGATAAGGGTTCGCGGGCAGAGCGAGCAATGCTTTCCAGtt                                                                   >  1:2611810/1‑93 (MQ=255)

                     gCTGGCAAGAATAGCTTCCGCTTCGGGAATATCGCCTTTCAAAATAGCTGAAGATAAGGGTTCGCGGGCAGAGCGAGCAATGCTTTCCAGTTg                                                                  <  1:5050782/93‑1 (MQ=255)

                     gCTGGCAAGAATAGCTTCCGCTTCGGGAATATCGCCTTTCAAAATAGCTGAAGATAAGGGTTCGCGGGCAGAGCGAGCAATGCTTTCCAGTTg                                                                  <  2:1747363/93‑1 (MQ=255)

                       tGGCAAGAATAGCTTCCGCTTCGGGAATATCGCCTTTCAAAATAGCTGAAGATAAGGGTTCGCGGGCAGAGCGAGCAATGCTTTCCAGTTGCg                                                                >  2:6707914/1‑93 (MQ=255)

                        ggCAAGAATAGCTTCCGCTTCGGGAATATCGCCTTTCAAAATAGCTGAAGATAAGGGTTCGCGGGCAGAGCGAGCAATGCTTTCCAGTTGCGt                                                               <  1:4480336/93‑1 (MQ=255)

                         gCAAGAATAGCTTCCGCTTCGGGAATATCGCCTTTCAAAATAGCTGAAGATAAGGGTTCGCGGGCAGAGCGAGCAATGCTTTCCAGTTGCGTa                                                              >  1:3382327/1‑93 (MQ=255)

                          cAAGAATAGCTTCCGCTTCGGGAATATCTCCTTTCAAAATAGCTGAAGATAAGGGTTCGCGGGCAGAGCGAGCAATGCTTTCCAGTTGCGTac                                                             >  1:5332582/1‑92 (MQ=255)

                           aaGAATAGCTTCCGCTTCGGGAATATCGCCTTTTAAAATAGCTGAAGATAAGGGTTCGCGGGCAGAGCGAGCAATGCTTTCCAGTTGCGTAGc                                                            >  1:827845/1‑93 (MQ=255)

                           aaGAATAGCTTCCGCTTCGGGAATATCGCCTTTCAAAATAGCTGAAGATAAGGGTTCGCGGGCAGAGCGAGCAATGCTTTCCAGTTGCGTAGc                                                            >  1:1666706/1‑93 (MQ=255)

                            aGAATAGCTTCCGCTTCGGAAATATCGCCTTTCAAAATAGCTGAAGATAAGGGTTCGCGGGCAGAGCGAGCAATGCTTTCCAGTTGCGTAGcc                                                           <  1:2853083/93‑1 (MQ=255)

                             gAATAGCTTCCGCTTCGGGAATATCGCCTTTCAAAATAGCTGAAGATAAGGGTTCGCGGGCAGAGCGAGCAATGCTTTCCAGTTGCGTaatcg                                                          >  1:637789/1‑89 (MQ=255)

                             gAATAGCTTCCGCTTCGGGAATATCGCCTTTCAAAATAGCTGAAGATAAGGGTTCGCGGGCAGAGCGAGCAATGCTTTCCAGTTGCGTAGCCg                                                          <  1:4130178/93‑1 (MQ=255)

                              aaTAGCTTCCGCTTCGGGAATATCGCCTTTCAAAATAGCTGAAGATAAGGGTTCGCGGGCAGAGCGAGCAATGCTTTCCAGTTGCGTAGCCgt                                                         <  2:5434666/93‑1 (MQ=255)

                              aaTAGCTTCCGCTTCGGGAATATCGCCTTTCAAAATAGCTGAAGATAAGGGTTCGCGGGCAGAGCGAGCAATGCTTTCCAGTTGCGTAGCCgt                                                         <  1:2236217/93‑1 (MQ=255)

                              aaTAGCTTCCGCTTCGGGAATATCGCCTTTCAAAATAGCTGAAGATAAGGGTTCGCGGGCAGAGCGAGCAATGCTTTCCAGTTGCGTAGCCgt                                                         >  2:4174028/1‑93 (MQ=255)

                                tAGCTTCCGCTTCGGGAATATCGCCTTTCAAAATAGCTGAAGATAAGGGTTCGCGGGCAGAGCGAGCAATGCTTTCCAGTTGCGTAGCCgtgt                                                       >  2:4884707/1‑93 (MQ=255)

                                tAGCTTCCGCTTCGGGAATATCGCCTTTCAAAATAGCTGAAGATAAGGGTTCGCGGGCAGAGCGAGCAATGCTTTCCAGTTGCGTAGCCgtgt                                                       >  1:4853212/1‑93 (MQ=255)

                                 aGCTTCCGCTTCGGGAATATCGCCTTTCAAAATAGCTGAAGATAAGGGTTCGCGGGCAGAGCGAGCAATGCTTTCCAGTTGCGTAGCCGTGtt                                                      <  2:4242023/93‑1 (MQ=255)

                                 aGCTTCCGCTTCGGGAATATCGCCTTTCAAAATAGCTGAAGATAAGGGTTCGCGGGCAGAGCGAGCAATGCTTTCCAGTTGCGTAGCCGTGtt                                                      >  1:1000258/1‑93 (MQ=255)

                                  gCTTCCGCTTCGGGAATATCGCCTTTCAAAATAGCTGAAGATAAGGGTTCGCGGGCAGAGCGAGCAATGCTTTCCAGTTGCGTAGCCGTGTta                                                     >  1:3118278/1‑93 (MQ=255)

                                  gCTTCCGCTTCGGGAATATCGCCTTTCAAAATAGCTGAAGATAAGGGTTCGCGGGCAGAGCGAGCAATGCTTTCCAGTTGCGTAGCCGTGTta                                                     >  1:3802504/1‑93 (MQ=255)

                                  gCTTCCGCTTCGGGAATATCGCCTTTCAAAATAGCTGAAGATAAGGGTTCGCGGGCAGAGCGAGCAATGCTTTCCAGTTGCGTAGCCGTGTta                                                     <  1:5757018/93‑1 (MQ=255)

                                  gCTTCCGCTGCGGGAATATCGCCTTTCAAAATAGTTGAAGATAAGGGTTCGCGGGCAGAGCGAGCAATGCTTTCCAGTTGCGTAGCCGTGTta                                                     <  1:34230/93‑1 (MQ=255)

                                   cTTCCGCTTCGGGAATATCGCCTTTCAAAATAGCTGAAGATAAGGGTTCGCGGGCAGAGCGAGCAATGCTTTCCAGTTGCGTAGCCGTGTtat                                                    <  1:3202083/93‑1 (MQ=255)

                                    ttCCGCTTCGGGAATATCGCCTTTCAAAATAGCTGAAGATAAGGGTTCGCGGGCAGAGCGAGCAATGCTTTCCAGTTGCGTAGCCGTGTtata                                                   >  2:2535231/1‑93 (MQ=255)

                                     tCCGCTTCGGGAATATCGCCTTTCAAAATAGCTGAAGATAAGGGTTCGCGGGCAGAGCGAGCAATGCTTTCCAGTTGCGTAGCCGTGTTATAg                                                  <  2:763973/93‑1 (MQ=255)

                                     tCCGCTTCGGGAATATCGCCTTTCAAAATAGCTGAAGATAAGGGTTCGCGGGCAGAGCGAGCAATGCTTTCCAGTTGCGTAGCCGTGTTATAg                                                  <  1:1860979/93‑1 (MQ=255)

                                     tCCGCTTCGGGAATATCGCCTTTCAAAATAGCTGAAGATAAGGGTTCGCGGGCAGAGCGAGCAATGCTTTCCAGTTGCGTAGCCGTGTTATAg                                                  <  2:4492849/93‑1 (MQ=255)

                                       cGCTTCGGGAATATCGCCTTTCAAAATAGCTGAAGATAAGGGTTCGCGGGCAGAGCGAGCAATGCTTTCCAGTTGCGTAGCCGTGTTATAGCg                                                >  2:2934996/1‑93 (MQ=255)

                                        gCTTCGGGAATATCGCCTTTCAAAATAGCTGAAGATAAGGGTTCGCGGGCAGAGCGAGCAATGCTTTCCAGTTGCGTAGCCGTGTTATAGCGa                                               >  1:16498/1‑93 (MQ=255)

                                        gCTTCGGGAATATCGCCTTTCAAAATAGCTGAAGATAAGGGTTCGCGGGCAGAGCGAGCAATGCTTTCCAGTTGCGTAGCCGTGTTATAGCGa                                               <  2:4636276/93‑1 (MQ=255)

                                          ttCGGGAATATCGCCTTTCAAAATAGCTGAAGATAAGGGTTCGCGGGCAGAGCGAGCAATGCTTTCCAGTTGCGTAGCCGTGTTATAGCGAtt                                             <  2:2925408/93‑1 (MQ=255)

                                           tCGGGAATATCGCCTTTCAAAATAGCTGAAGATAAGGGTTCGCGGGCAGAGCGAGCAATGCTTTCCAGTTGCGTAGCCGTGTTATAGCGATTc                                            >  2:1395910/1‑93 (MQ=255)

                                            cGGGAATATCGCCTTTCAAAATAGCTGAAGATAAGGGTTCGCGGGCAGAGCGAGCAATGCTTTCCAGTTGCGTAGCCGTGTTATAGCGATTct                                           <  1:4947222/93‑1 (MQ=255)

                                              ggAATATCGCCTTTCAAAATAGCTGAAGATAAGGGTTCGCGGGCAGAGCGAGCAATGCTTTCCAGTTGCGTAGCCGTGTTATAGCGATTctgc                                         >  1:252994/1‑93 (MQ=255)

                                              ggAATATCGCCTTTCAAAATAGCTGAAGATAAGGGTTCGCGGGCAGAGCGAGCAATGCTTTCCAGTTGCGTAGCCGTGTTATAGCGATTctgc                                         >  1:6153474/1‑93 (MQ=255)

                                              ggAATATCGCCTTTCAAAATAGCTGAAGATAAGGGTTCGCGGGCAGAGCGAGCAATGCTTTCCAGTTGCGTAGCCGTGTTATAGCGATTctgc                                         <  1:2659796/93‑1 (MQ=255)

                                              ggAATATCGCCTTTCAAAATAGCTGAAGATAAGGGTTCGCGGGCAGAGCGAGCAATGCTTTCCAGTTGCGTAGCCGTGTTATAGCGATTctgc                                         <  2:6122494/93‑1 (MQ=255)

                                               gAATATCGCCTTTCAAAATAGCTGAAGATAAGGGTTCGCGGGCAGAGCGAGCAATGCTTTCCAGTTGCGTAGCCGTGTTATAGCGATTctgct                                        >  1:3605154/1‑93 (MQ=255)

                                                aaTATCGCCTTTCAAAATAGCTGAAGATAAGGGTTCGCGGGCAGAGCGAGCAATGCTTTCCAGTTGCGTAGCCGTGTTATAGCGATTctgctg                                       >  2:1587506/1‑93 (MQ=255)

                                                  tatCGCCTTTCAAAATAGCTGAAGATAAGGGTTCGCGGGCAGAGCGAGCAATGCTTTCCAGTTGCGTAGCCGTGTTATAGCGATTCTGCTggc                                     >  2:4446896/1‑92 (MQ=255)

                                                   atCGCCTTTCAAAATAGCTGAAGATAAGGGTTCGCGGGCAGAGCGAGCAATGCTTTCCAGTTGCGTAGCCGTGTTATAGCGATTCTGCTTGAc                                    >  1:1545130/1‑93 (MQ=255)

                                                   atCGCCTTTCAAAATAGCTGAAGATAAGGGTTCGCGGGCAGAGCGAGCAATGCTTTCCAGTTGCGTAGCCGTGTTATAGCGATTCTGCTGGAc                                    <  1:733833/93‑1 (MQ=255)

                                                   atCGCCTTTCAAAATAGCTGAAGATAAGGGTTCGCGGGCAGAGCGAGCAATGCTTTCCAGTCGCGTAGCCGTGTTATAGCGATTCTGCTGGAc                                    >  1:943228/1‑93 (MQ=255)

                                                    tCGCCTTTCAAAATAGCTGAAGATAAGGGTTCGCGGGCAGAGCGAGCAATGCTTTCCAGTTGCGTAGCCGTGTTATAGCGATTCTGCTGGAcc                                   >  1:2843474/1‑93 (MQ=255)

                                                     cGCCTTTCAAAATAGCTGAAGATAAGGGTTCGCGGGCAGAGCGAGCAATGCTTTCCAGTTGCGTAGCCGTGTTATAGCGATTCTGCTGGACCa                                  >  1:2443973/1‑93 (MQ=255)

                                                       ccTTTCAAAATAGCTGAAGATAAGGGTTCGCGGGCAGAGCGAGCAATGCTTTCCAGTTGCGTAGCCGTGTTATAGCGATTCTGCTGGACCAGa                                >  1:2280651/1‑93 (MQ=255)

                                                       ccTTTCAAAATAGCTGAAGATAAGGGTTCGCGGGCAGAGCGAGCAATGCTTTCCAGTTGCGTAGCCGTGTTATAGCGATTCTGCTGGACCAGa                                <  2:344291/93‑1 (MQ=255)

                                                        cTTTCAAAATAGCTGAAGATAAGGGTTCGCGGGCAGAGCGAGCAATGCTTTCCAGTTGCGTAGCCGTGTTATAGCGATTCTGCTGGACCAGAt                               >  1:1950560/1‑93 (MQ=255)

                                                        cTTTCAAAATAGCTGAAGATAAGGGTTCGCGGGCAGAGCGAGCAATGCTTTCCAGTTGCGTAGCCGTGTTATAGCGATTCTGCTGGACCAGAt                               >  2:3678720/1‑93 (MQ=255)

                                                        cTTTCAAAATAGCTGAAGATAAGGGTTCGCGGGCAGAGCGAGCAATGCTTTCCAGTTGCGTAGCCGTGTTATAGCGATTCTGCTGGACCAGAt                               >  1:3290195/1‑93 (MQ=255)

                                                        cTTTCAAAATAGCTGAAGATAAGGGTTCGCGGGCAGAGCGAGCAATGCTTTCCAGTTGCGTAGCCGTGTTATAGCGATTCTGCTGGACCAGAt                               <  1:6650788/93‑1 (MQ=255)

                                                        cTTTCAAAATAGCTGAAGATAAGGGTTCGCGGGCAGAGCGAGCAATGCTTTCCAGTTGCGTAGCCGTGTTATAGCGATTCTGCTGGACCAGAt                               <  2:6385534/93‑1 (MQ=255)

                                                         tttCAAAATAGCTGAAGATAAGGGTTCGCGGGCAGAGCGAGCAATGCTTTCCAGTTGCGTAGCCGTGTTATAGCGATTCTGCTGGACCAGATg                              <  1:6228701/93‑1 (MQ=255)

                                                         tttCAAAATAGCTGAAGATAAGGGTTCGCGGGCAGAGCGAGCAATGCTTTCCAGTTGCGTAGCCGTGTTATAGCGATTCTGCTGGACCAGATg                              >  2:3066561/1‑93 (MQ=255)

                                                         tttCAAAATAGCTGAAGATAAGGGTTCGCGGGCAGAGCGAGCAATGCTTTCCAGTTGCGTAGCCGTGTTATAGCGATTCTGCTGGACCAGATg                              <  1:2979063/93‑1 (MQ=255)

                                                           tCAAAATAGCTGAAGATAAGGGTTCGCGGGCAGAGCGAGCAATGCTTTCCAGTTGCGTAGCCGTGTTATAGCGATTCTGCTGGACCAGATGGa                            >  1:4634796/1‑93 (MQ=255)

                                                           tCAAAATAGCTGAAGATAAGGGTTCGCGGGCAGAGCGAGCAATGCTTTCCAGTTGCGTAGCCGTGTTATAGCGATTCTGCTGGACCAGATGGa                            >  1:1244163/1‑93 (MQ=255)

                                                            cAAAATAGCTGAAGATAAGGGTTCGCGGGCAGAGCGAGCAATGCTTTCCAGTTGCGTAGCCGTGTTATAGCGATTCTGCTGGACCAGATGGaa                           <  1:6317528/93‑1 (MQ=255)

                                                            cAAAATAGCTGAAGATAAGGGTTCGCGGGCAGAGCGAGCAATGCTTTCCAGTTGCGTAGCCGTGTTATAGCGATTCTGCTGGACCAGATGGaa                           <  1:5165332/93‑1 (MQ=255)

                                                             aaaaTAGCTGAAGATAAGGGTTCGCGGGCAGAGCGAGCAATGCTTTCCAGTTGCGTAGCCGTGTTATAGCGATTCTGCTGGACCAGATGGAAc                          >  2:6250116/1‑93 (MQ=255)

                                                             aaaaTAGCTGAAGATAAGGGTTCGCGGGCAGAGCGAGCAATGCTTTCCAGTTGCGTAGCCGTGTTATAGCGATTCTGCTGGACCAGATGGAAc                          <  2:1996965/93‑1 (MQ=255)

                                                             aaaaTAGCTGAAGATAAGGGTTCGCGGGCAGAGCGAGCAATGCTTTCCAGTTGCGTAGCCGTGTTATAGCGATTCTGCTGGACCAGATGGAAc                          <  1:1128848/93‑1 (MQ=255)

                                                             aaaaTAGCTGAAGATAAGGGTTCGCGGGCAGAGCGAGCAATGCTTTCCAGTTGCGTAGCCGTGTTATAGCGATTCTGCTGGACCAGATGGAAc                          >  2:535012/1‑93 (MQ=255)

                                                              aaaTAGCTGAAGATAAGGGTTCGCGGGCAGAGCGAGCAATGCTTTCCAGTTGCGTAGCCGTGTTATAGCGATTCTGCTGGACCAGATGGAACa                         <  2:621920/93‑1 (MQ=255)

                                                              aaaTAGCTGAAGAGAAGGGTTCGCGGGCAGAGCGAGCAATGCTTTCCAGTTGCGTAGCCGTGTTATAGCGATTCTGCTGGACCAGATGTAACa                         >  1:6602688/1‑93 (MQ=255)

                                                                aTAGCTGAAGATAAGGGTTCGCGGGCAGAGCGAGCAATGCTTTCCAGTTGCGTAGCCGTGTTATAGCGATTCTGCTGGACCAGATGGAACAGc                       >  1:541542/1‑93 (MQ=255)

                                                                aTAGCTGAAGATAAGGGTTCGCGGGCAGAGCGAGCAATGCTTTCCAGTTGCGTAGCCGTGTTATAGCGATTCTGCTGGACCAGATGGAACAGc                       >  1:4150402/1‑93 (MQ=255)

                                                                 tAGCTGAAGATAAGGGTTCGCGGGCAGAGCGAGCAATGCTTTCCAGTTGCGTAGCCGTGTTATAGCGATTCTGCTGGACCAGATGGAACAGCa                      >  2:263691/1‑93 (MQ=255)

                                                                 tAGCTGAAGATAAGGGTTCGCGGGCAGAGCGAGCAATGCTTTCCAGTTGCGTAGCCGTGTTATAGCGATTCTGCTGGACCAGATGGAACAGCa                      <  2:2278609/93‑1 (MQ=255)

                                                                  aGCTGAAGATAAGGGTTCGCGGGCAGAGCGAGCAATGCTTTCCAGTTGCGTAGCCGTGTTATAGCGATTCTGCTGGACCAGATGGAACAGCaa                     >  2:1123990/1‑93 (MQ=255)

                                                                  aGCTGAAGATAAGGGTTCGCGGGCAGAGCGAGCAATGCCTTCCAGTTGCGTAGCCGTGTTATAGCGATTCTGCTGGACCAGATGGACCAGCaa                     <  1:1041252/93‑1 (MQ=255)

                                                                   gCTGAAGATAAGGGTTCGCGGGCAGAGCGAGCAATGCTTTCCAGTTGCGTAGCCGTGTTATAGCGATTCTGCTGGACCAGATGGAACAGCaaa                    >  2:4718831/1‑93 (MQ=255)

                                                                    cTGAAGATAAGGGTTCGCGGGCAGAGCGAGCAATGCTTTCCAGTTGCGTAGCCGTGTTATAGCGATTCTGCTGGACCAGATGGAACAGCaaaa                   <  2:5846305/93‑1 (MQ=255)

                                                                    cTGAAGATAAGGGTTCGCGGGCAGAGCGAGCAATGCTTTCCAGTTGCGTAGCCGTGTTATAGCGATTCTGCTGGACCAGATGGAACAGCaaaa                   >  2:4047989/1‑93 (MQ=255)

                                                                        aGATAAGGGTTCGCGGGCAGAGCTAGCAATGCTTTCCAGTTGCGTAGCGGTGTTATAGCGATTCTGCTGGACCAGATGGAACAGCAAAACGGt               <  1:1822499/93‑1 (MQ=255)

                                                                        aGATAAGGGTTCGCGGGCAGAGCGAGCAATGCTTTCCAGTTGCGTAGCCGTGTTATAGCGATTCTGCTGGACCAGATGGAACAGCAAAACGGt               <  1:893142/93‑1 (MQ=255)

                                                                        aGATAAGGGTTCGCGGGCAGAGCGAGCAATGCTTTCCAGTTGCGTAGCCGTGTTATAGCGATTCTGCTGGACCAGATGGAACAGCAAAACGGt               <  2:950753/93‑1 (MQ=255)

                                                                         gATAAGGGCTCGCGGGCAGAGCGAGCAATGCTTTCCAGTTGCGTAGCCGTGTTATAGCGATTCTGCTGGACCAGATGGAACAGCAAAACGGTg              >  1:5482443/1‑93 (MQ=255)

                                                                              gggTTCGCGGGCAGAGCGAGCAATGCTTTCCAGTTGCGTAGCCGTGTTATAGCGATTCTGCTGGACCAGATGGAACAGCAAAACGGTGCaaaa         <  2:6084401/93‑1 (MQ=255)

                                                                                gTTCGCGGGCAGAGCGAGCAATGCTTTCCAGTTGCGTAGCCGTGTTATAGCGATTCTGCTGGACCAGATGGAACAGCAAAACGGTGCaaaaaa       <  2:2474291/93‑1 (MQ=255)

                                                                                 ttCGCGGGCAGAGCGAGCAATGCTTTCCAGTTGCGTAGCCGTGTTATAGCGATTCTGCTGGACCAGATGGAACAGCAAAACGGTGCAAAAAAt      >  1:5703819/1‑93 (MQ=255)

                                                                                     cgGGCAGAGCGAGCAATGCTTTCCAGTTGCGTAGCCGTGTTATAGCGATTCTGCTGGACCAGATGGAACAGCAAAACGGTGCAAAAAATaaaa  <  2:5125695/93‑1 (MQ=255)

GACCACGCCTGCCGGTTTAATGCTGGCAAGAATAGCTTCCGCTTCGGGAATATCGCCTTTCAAAATAGCTGAAGATAAGGGTTCGCGGACAGAGCGAGCAATGCTTTCCAGTTGCGTAGCCGTGTTATAGCGATTCTGCTGGACCAGATGGAACAGCAAAACGGTGCAAAAAATAAAA  >  NC_000913/3683003‑3683180

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 19 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`