BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,754,310	C→A	R382M (AGG→ATG)	yiaY ←	putative oxidoreductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,754,310	0	C	A	100.0%	1172.8 / NA	311	R382M (AGG→ATG)	yiaY	putative oxidoreductase
Reads supporting (aligned to +/- strand): ref base C (0/0); new base A (188/123); total (188/123)

Only 100 of 316 total aligned reads displayed.

GACAGCTATCACGAATTTACGGGCAAGGGTTACAGAGATAGTTGAAAAAGCGGCTAACAATTTGCCAGCCGTTGTGGAAATGATGATTACCTCGCTGCATAAATCGCCACAATTTCTTCGTGAGTTGCCTGGATCGGGTTAGTAAAGCCACAGGCATCTTTCAGGGCATTAGTCGCCAAT  >  NC_000913/3754220‑3754399

gACAGCTATCACGAATTTACGGGCAAGGGTTACAGAGATAGTTGAAAAAGCGGCTAACAATTTGCCAGCCGTTGTGGAAATGATGATTACATc                                                                                         <  1:5937275/93‑1 (MQ=255)

 aCAGCTATCACGAATTTACGGGCAAGGGTTACAGAGATAGTTGAAAAAGCGGCTAACAATTTGCCAGCCGTTGTGGAAATGATGATTACATCg                                                                                        >  2:4233207/1‑93 (MQ=255)

   aGCTATCACGAATTTACGGGCAAGGGTTACAGAGATAGTTGAAAAAGCGGCTAACAATTTGCCAGCCGTTGTGGAAATGATGATTACATCGCt                                                                                      >  2:361250/1‑93 (MQ=255)

     cTATCACGAATTTACGGGCAAGGGTTACAGAGATAGTTGAAAAAGCGGCTAACAATTTGCCAGCCGTTGTGGAAATGATGATTACATCGCTGc                                                                                    >  2:6239401/1‑93 (MQ=255)

      tATCACGAATTTACGGGCAAGGGTTACAGAGATAGTTGAAAAAGCGGCTAACAATTTGCCAGCCGTTGTGGAAATGATGATTACATCGCTGCg                                                                                   >  1:5526564‑M1/1‑92 (MQ=255)

       aTCACGAATTTACGGGCAAGGGTTACAGAGATAGTTGAAAAAGCGGCTAACAATTTGCCAGCCGTTGTGGAAATGATGATTACATCGCTGCgc                                                                                  >  2:5220123‑M1/1‑91 (MQ=255)

       aTCACGAATTTACGGGCAAGGGTTACAGAGATAGTTGAAAAAGCGGCTAACAATTTGCCAGCCGTTGTGGAAATGATGATTACATCGCTGCgc                                                                                  >  2:3542329‑M1/1‑91 (MQ=255)

       aTCACGAATTTACGGGCAAGGGTTACAGAGATAGTTGAAAAAGCGGCTAACAATTTGCCAGCCGTTGTGGAAATGATGATTACATCGCTGCgc                                                                                  <  1:906339‑M1/93‑3 (MQ=255)

        tCACGAATTTACGGGCAAGGGGTACAGAGATAGTTGAAAAAGCGGCTAACAATTTGCCAGCCGTTGTGGAAATGATGATTACATCGCTGCgcg                                                                                 >  1:1218294‑M1/1‑90 (MQ=255)

          aCGAATTTACGGGCAAGGGTTACAGAGATAGTTGAAAAAGCGGCTAACAATTTGCCAGCCGTTGTGGAAATGATGATTACATCGCTGCgcgat                                                                               <  1:3036418‑M1/93‑6 (MQ=255)

             aaTTTACGGGCAAGGGTTACAGAGATAGTTGAAAAAGCGGCTAACAATTTGCCAGCCGTTGTGGAAATGATGATTACATCGCTGCgcgataaa                                                                            >  2:380787‑M1/1‑85 (MQ=255)

               tttACGGGCAAGGGTTACAGAGATAGTTGAAAAAGCGGCTAACAATTTGCCAGCCGTTGTGGAAATGATGATTACATCGCTGCgcgataaatc                                                                          >  2:6540752‑M1/1‑83 (MQ=255)

               tttACGGGCAAGGGTTACAGAGATAGTTGAAAAAGCGGCTAACAATTTGCCAGCCGTTGTGGAAATGATGATTACATCGCTGCgcgataaatc                                                                          >  1:5677100‑M1/1‑83 (MQ=255)

                 tACGGGCAAGGGTTACAGAGATAGTTGAAAAAGCGGCTAACAATTTGCCAGCCGTTGTGGAAATGATGATTACATCGCTGCgcgataaatcgc                                                                        >  1:3976102‑M1/1‑81 (MQ=255)

                 tACGGGCAAGGGTTACAGAGATAGTTGAAAAAGCGGCGAACAATTTGCCAGCCGTTGTGGAAATGATGATTACATCGCTGCgcgataaatcgc                                                                        >  2:1264608‑M1/1‑81 (MQ=255)

                     ggCAAGGGTTACAGAGATAGTTGAAAAAGCGGCTAACAATTTGCCAGCCGTTGTGGACATGATGATTACATCGCTGCgcgataaatcgccaca                                                                    >  1:1110894‑M1/1‑77 (MQ=255)

                      gCAAGGGTTACAGAGATAGTTGAAAAAGCGGCTAACAATTTGCCAGCCGTTGTGGAAATGATGATTACATCGCTGCgcgataaatcgccacaa                                                                   >  2:4187526‑M1/1‑76 (MQ=255)

                      gCAAGGGTTACAGAGATAGTTGAAAAAGCGGCTAACAATTTGCCAGCCGTTGTGGAAATGATGATTACATCGCTGCgcgataaatcgccacaa                                                                   <  1:4454690‑M1/93‑18 (MQ=255)

                        aaGGGTTACAGAGATAGTTGAAAAAGCGGCTAACAATTTGCCAGCCGTTGTGGAAATGATGATTACATCGCTGCgcgataaatcgccacaatt                                                                 >  1:3490254‑M1/1‑74 (MQ=255)

                         aGGGTTACAGAGATAGTTGAAAAAGCGGCTAACAATTTGCCAGCCGTTGTGGAAATGATGATTACATCGCTGCgcgataaatcgccacaattt                                                                >  1:1551759‑M1/1‑73 (MQ=255)

                         aGGGTTACAGAGATAGTTGAAAAAGCGGCTAACAATTTGCCAGCCGTTGTGGAAATGATGATTACATCGCTGCgcgataaatcgccacaattt                                                                <  2:3797145‑M1/93‑21 (MQ=255)

                         aGGGTTACAGAGATAGTTGAAAAAGCGGCTAACAATTTGCCAGCCGTTGTGGAAATGATGATTACATCGCTGCgcgataaatcgccacaattt                                                                >  1:1791793‑M1/1‑73 (MQ=255)

                          gggTTACAGAGATAGTTGAAAAAGCGGCTAACAATTTGCCAGCCGTTGTGGAAATGATGATTACATCGCTGCgcgataaatcgccacaatttc                                                               >  1:5806586‑M1/1‑72 (MQ=255)

                          gggTTACAGAGATAGTTGAAAAAGCGGCTAACAATTTGCCAGCCGTTGTGGAAATGATGATTACATCGCTGCgcgataaatcgccacaatttc                                                               >  2:4412783‑M1/1‑72 (MQ=255)

                          gggTTACAGAGATAGTTGAAAAAGCGGCTAACAATTTGCCAGCCGTTGTGGAAATGATGATTACATCGCTGCgcgataaatcgccacaatttc                                                               >  1:6162927‑M1/1‑72 (MQ=255)

                           gtttacAGAGATAGTTGAAAAAGCGGCTAACAATTTGCCAGCCGTTGTGGAAATGATGATTACATCGCTGCgcgataaatcgccacaatttct                                                              <  2:2781322‑M1/91‑23 (MQ=255)

                           ggTTACAGAGATAGTTGAAAAAGCGGCTAACAATTTGCCAGCCGTTGTGGAAATGATGATTACATCGCTGCgcgataaatcgccacaatttct                                                              >  1:4579024‑M1/1‑71 (MQ=255)

                           ggTTACAGAGATAGTTGAAAAAGCGGCTAACAATTTGCCAGCCGTTGTGGAAATGATGATTACATCGCTGCgcgataaatcgccacaatttct                                                              <  2:5328768‑M1/93‑23 (MQ=255)

                            gTTACAGAGATAGTTGAAAAAGCGGCTAACAATTTGCCAGCCGTTGTGGAAATGATGATTACATCGCTGCgcgataaatcgccacaatttctt                                                             <  2:2634790‑M1/93‑24 (MQ=255)

                            gTTACAGAGATAGTTGAAAAAGCGGCTAACAATTTGCCAGCCGTTGTGGAAATGATGATTACATCGCTGCgcgataaatcgccacaatttctt                                                             >  2:3373842‑M1/1‑70 (MQ=255)

                               aCAGAGATAGTTGAAAAAGCGGCTAACAATTTGCCAGCCGTTGTGGAAATGATGATTACATCGCTGCgcgataaatcgccacaatttcttcgt                                                          >  2:3288700‑M1/1‑67 (MQ=255)

                               aCAGAGATAGTTGAAAAAGCGGCTAACAATTTGCCAGCCGTTGTGGAAATGATGATTACATCGCTGCgcgataaatcgccacaatttcttcgt                                                          <  2:3091921‑M1/93‑27 (MQ=255)

                                 agagATAGTTGAAAAAGCGGCTAACAATTTGCCAGCCGTTGTGGAAATGATGATTACATCGCTGCgcgataaatcgccacaatttcttcgtga                                                        >  1:6028995‑M1/1‑65 (MQ=255)

                                 agagATAGTTGAAAAAGCGGCTAACAATTTGCCAGCCGTTGTGGAAATGATGATTACATCGCTGCgcgataaatcgccacaatttcttcgtga                                                        <  1:98057‑M1/93‑29 (MQ=255)

                                 agagATAGTTGAAAAAGCGGCTAACAATTTGCCAGCCGTTGTGGAAATGATGATTACATCGCTGCgcgataaatcgccacaatttcttcgtga                                                        >  2:3715057‑M1/1‑65 (MQ=255)

                                 agagATAGTAGAAAAAGCGGCTAACAATTTGCCAGCCGTTGTAGAAATGATGATTACATCGCTGCgcgataaatcgccacaatttcttcgtga                                                        <  2:4104607‑M1/93‑29 (MQ=255)

                                  gagaTAGTTGAAAAAGCGGCTAACAATTTGCCAGCCGTTGTGGAAATGATGATTACATCGCTGCgcgataaatcgccacaatttcttcgtgag                                                       <  2:4977309‑M1/93‑30 (MQ=255)

                                   agaTAGTTGAAAAAGCGGCTAACAATTTGCCAGCCGTTGTGGAAATGATGATTACATCGCTGCgcgataaatcgccacaatttcttcgtgagt                                                      <  1:5638752‑M1/93‑31 (MQ=255)

                                   agaTAGTTGAAAAAGCGGCTAACAATTTGCCAGCCGTTGTGGAAATGATGATTACATCGCTGCgcgataaatcgccacaatttcttcgtgagt                                                      >  1:3689772‑M1/1‑63 (MQ=255)

                                   agaTAGTTGAAAAAGCGGCTAACAATTTGCCAGCCGTTGTGGAAATGATGATTACATCGCTGCgcgataaatcgccacaatttcttcgtgagt                                                      <  1:3226379‑M1/93‑31 (MQ=255)

                                    gaTAGTTGAAAAAGCGGCTAACAATTTGCCAGCCGTTGTGGAAATGATGATTACATCGCTGCgcgataaatcgccacaatttcttcgtgagtt                                                     <  1:6504436‑M1/93‑32 (MQ=255)

                                    gaTAGTTGAAAAAGCGGCTAACAATTTGCCAGCCGTTGTGGAAATGATGATTACATCGCTGCgcgataaatcgccacaatttcttcgtgagtt                                                     <  1:3281381‑M1/93‑32 (MQ=255)

                                      tAGTTGAAAAAGCGGCTAACAATTTGCCAGCCGTTGTGGAAATGATGATTACATCGCTGCgcgataaatcgccacaatttcttcgtgagttgc                                                   >  1:4892609‑M1/1‑60 (MQ=255)

                                      tAGTTGAAAAAGCGGCTAACAATTTGCCAGCCGTTGTGGAAATGATGATTACATCGCTGCgcgataaatcgccacaatttcttcgtgagttgc                                                   <  1:4342942‑M1/93‑34 (MQ=255)

                                      tAGTTGAAAAAGCGGCTAACAATTTGCCAGCCGTTGTGGAAATGATGATTACATCGCTGCgcgataaatcgccacaatttcttcgtgagttgc                                                   >  1:6433441‑M1/1‑60 (MQ=255)

                                       aGTTGAAAAAGCGGCTAACAATTTGCCAGCCGTTGTGGAAATGATGATTACATCGCTGCgcgataaatcgccacaatttcttcgtgagttgcc                                                  <  2:1572835‑M1/93‑35 (MQ=255)

                                        gTTGAAAAAGCGGCTAACAATTTGCCAGCCGTTGTGGAAATGATGATTACATCGCTGCgcgataaatcgccacaatttcttcgtgagttgcct                                                 >  2:640397‑M1/1‑58 (MQ=255)

                                        gTTGAAAAAGCGGCTAACAATTTGCCAGCCGTTGTGGAAATGATGATTACATCGCTGCgcgataaatcgccacaatttcttcgtgagttgcct                                                 >  1:4657887‑M1/1‑58 (MQ=255)

                                        gTTGAAAAAGCGGCTAACAATTTGCCAGCCGTTGTGGAAATGATGATTACATCGCTGCgcgataaatcgccacaatttcttcgtgagttgcct                                                 >  1:5177882‑M1/1‑58 (MQ=255)

                                           gAAAAAGCGGCTAACAATTTGCCAGCCGTTGTGGAAATGATGATTACATCGCTGCgcgataaatcgccacaatttcttcgtgagttgcctgga                                              >  2:348248‑M1/1‑55 (MQ=255)

                                            aaaaaGCGGCTAACAATTTGCCAGCCGTTGTGGAAATGATGATTACATCGCTGCgcgataaatcgccacaatttcttcgtgagttgcctggat                                             <  1:2162913‑M1/93‑40 (MQ=255)

                                             aaaaGCGGCTAACAATTTGCCAGCCGTTGTGGAAATGATGATTACATCGCTGCgcgataaatcgccacaatttcttcgtgagttgcctggatc                                            >  1:2949608‑M1/1‑53 (MQ=255)

                                              aaaGCGGCTAACAATTTGCCAGCCGTTGTGGAAATGATGATTACATCGCTGCgcgataaatcgccacaatttcttcgtgagttgcctggatcg                                           <  2:3762844‑M1/93‑42 (MQ=255)

                                              aaaGCGGCTAACAATTTGCCAGCCGTTGTGGAAATGATGATTACATCGCTGCgcgataaatcgccacaatttcttcgtgagttgcctggatcg                                           >  1:6223279‑M1/1‑52 (MQ=255)

                                               aaGCGGCTAACAATTTGCCAGCCGTTGTGGAAATGATGATTACATCGCTGCgcgataaatcgccacaatttcttcgtgagttgcctggatcgg                                          <  1:6654043‑M1/93‑43 (MQ=255)

                                               aaGCGGCTAACAATTTGCCAGCCGTTGTGGAAATGATGATTACATCGCTGCgcgataaatcgccacaatttcttcgtgagttgcctggatcgg                                          >  2:4864235‑M1/1‑51 (MQ=255)

                                                 gCGGCTAACAATTTGCCAGCCGTTGTGGAAATGATGATTACATCGCTGCgcgataaatcgccacaatttcttcgtgagttgcctggatcgggt                                        >  1:6406007‑M1/1‑49 (MQ=255)

                                                   ggCTAACAATTTGCCAGCCGTTGTGGAAATGATGATTACATCGCTGCgcgataaatcgccacaatttcttcgtgagttgcctggatcgggtta                                      >  1:3851448‑M1/1‑47 (MQ=255)

                                                   ggCTAACAATTTGCCAGCCGTTGTGGAAATGATGATTACATCGCTGCgcgataaatcgccacaatttcttcgtgagttgcctggatcgggtta                                      <  2:2684918‑M1/93‑47 (MQ=255)

                                                    gCTAACAATTTGCCAGCCGTTGTGGAAATGATGATTACATCGCTGCgcgataaatcgccacaatttcttcgtgagttgcctggatcgggttag                                     >  2:1475806‑M1/1‑46 (MQ=255)

                                                     cTAACAATTTGCCAGCCGTTGTGGAAATGATGATTACATCGCTGCgcgataaatcgccacaatttcttcgtgagttgcctggatcgggttagt                                    <  2:4558656‑M1/93‑49 (MQ=255)

                                                      tAACAATTTGCCAGCCGTTGTGGAAATGATGATTACATCGCTGCgcgataaatcgccacaatttcttcgtgagttgcctggatcgggttagta                                   >  1:4735298‑M1/1‑44 (MQ=255)

                                                       aaCAATTTGCCAGCCGTTGTGGAAATGATGATTACATCGCTGCgcgataaatcgccacaatttcttcgtgagttgcctggatcgggttagtaa                                  >  1:2342733‑M1/1‑43 (MQ=255)

                                                       aaCAATTTGCCAGCCGTTGTGGAAATGATGATTACATCGCTGCgcgataaatcgccacaatttcttcgtgagttgcctggatcgggttagtaa                                  <  2:6147819‑M1/93‑51 (MQ=255)

                                                        aCAATTTGCCAGCCGTTGTGGAAATGATGATTACATCGCTGCgcgataaatcgccacaatttcttcgtgagttgcctggatcgggttagtaaa                                 >  2:1202528‑M1/1‑42 (MQ=255)

                                                         cAATTTGCCAGCCGTTGTGGAAATGATGATTACATCGCTGCgcgataaatcgccacaatttcttcgtgagttgcctggatcgggttagtaaag                                >  2:5932668‑M1/1‑41 (MQ=255)

                                                         cAATTTGCCAGCCGTTGTGGAAATGATGATTACATCGCTGCgcgataaatcgccacaatttcttcgtgagttgcctggatcgggttagtaaag                                >  1:1719024‑M1/1‑41 (MQ=255)

                                                           aTTTGCCAGCCGTTGTGGAAATGATGATTACATCGCTGCgcgataaatcgccacaatttcttcgtgagttgcctggatcgggttagtaaagcc                              >  1:1277340‑M1/1‑39 (MQ=255)

                                                           aTTTGCCAGCCGTTGTGGAAATGATGATTACATCGCTGCgcgataaatcgccacaatttcttcgtgagttgcctggatcgggttagtaaagcc                              >  2:3916142‑M1/1‑39 (MQ=255)

                                                               gCCAGCCGTTGTGGAAATGATGATTACATGGCTGGgcgataaatcgccacaatttcttggtgagttgcctggagcgggttagtaaagccacag                          >  2:2930704‑M1/1‑35 (MQ=255)

                                                               gCCAGCCGTTGTGGAAATGATGATTACATCGCTGCgcgataaatcgccacaatttcttcgtgagttgcctggatcgggttagtaaagccacag                          <  1:1393823‑M1/93‑59 (MQ=255)

                                                                 cAGCCGTTGTGGAAATGATGATTACATCGCTGCgcgataaatcgccacaatttcttcgtgagttgcctggatcgggttagtaaagccacaggc                        >  1:6070565‑M1/1‑33 (MQ=255)

                                                                  aGCCGTTGTGGAAATGATGATTACATCGCTGCgcgataaatcgccacaatttcttcgtgagttgcctggatcgggttagtaaagccacaggca                       >  1:2569247‑M1/1‑32 (MQ=255)

                                                                  aGCCGTTGTGGAAATGATGATTACATCGCTGCgcgataaatcgccacaatttcttcgtgagttgcctggatcgggttagtaaagccacaggca                       >  2:5134301‑M1/1‑32 (MQ=255)

                                                                  aGCCGTTGTGGAAATGATGATTACATCGCTGCgcgataaatcgccacaatttcttcgtgagttgcctggatcgggttagtaaagccacaggca                       <  2:6034084‑M1/93‑62 (MQ=255)

                                                                  aGCCGTTGTGGAAATGATGATTACATCGCTGCgcgataaatcgccacaatttcttcgtgagttgcctggatcgggttagtaaagccacaggca                       <  1:4117124‑M1/93‑62 (MQ=255)

                                                                   gCCGTTGTGGAAATGATGATTACATCGCTGCgcgataaatcgccacaatttcttcgtgagttgcctggatcgggttagtaaagccacaggcat                      <  2:4474847‑M1/93‑63 (MQ=255)

                                                                   gCCGTTGTGGAAATGATGATTACATCGCTGCgcgataaatcgccacaatttcttcgtgagttgcctggatcgggttagtaaagccacaggcat                      >  2:6462664‑M1/1‑31 (MQ=255)

                                                                   gCCGTTGTGGAAATGATGATTACATCGCTGCgcgataaatcgccacaatttcttcgtgagttgcctggatcgggttagtaaagccacaggcat                      >  1:6613466‑M1/1‑31 (MQ=255)

                                                                     cGTTGTGGAAATGATGATTACATCGCTGCgcgataaatcgccacaatttcttcgtgagttgcctggatcgggttagtaaagccacaggcatct                    <  1:4211033‑M1/93‑65 (MQ=255)

                                                                      gTTGTGGAAATGATGATTACATCGCTGCgcgataaatcgccacaatttcttcgtgagttgcctggatcgggttagtaaagccacaggcatctt                   >  2:736667‑M1/1‑28 (MQ=255)

                                                                       ttGTGGAAATGATGATTACATCGCTGCgcgataaatcgccacaatttcttcgtgagttgcctggatcgggttagtaaagccacaggcatcttt                  >  2:2948356‑M1/1‑27 (MQ=255)

                                                                        tgtgGAAATGATGATTACATCGCTGCgcgataaatcgccacaatttcttcgtgagttgcctggatcgggttagtaaagccacaggcatctttc                 >  1:3598852‑M1/1‑26 (MQ=255)

                                                                        tgtgGAAATGATGATTACATCGCTGCgcgataaatcgccacaatttcttcgtgagttgcctggatcgggttagtaaagccacaggcatctttc                 >  2:2481408‑M1/1‑26 (MQ=255)

                                                                          tgGAAATGATGATTACATCGCTGCgcgataaatcgccacaatttcttcgtgagttgcctggatcgggttagtaaagccacaggcatctttcag               <  2:5667881‑M1/93‑70 (MQ=255)

                                                                          tgGAAATGATGATTACATCGCTGCgcgataaatcgccacaatttcttcgtgagttgcctggatcgggttagtaaagccacaggcatctttcag               >  1:1653237‑M1/1‑24 (MQ=255)

                                                                           ggAAATGATGATTACATCGCTGCgcgataaatcgccacaatttcttcgtgagttgcctggatcgggttagtaaagccacaggcatctttcagg              <  1:2766526‑M1/93‑71 (MQ=255)

                                                                           ggAAATGATGATTACATCGCTGCgcgataaatcgccacaatttcttcgtgagttgcctggatcgggttagtaaagccacaggcatctttcagg              >  1:2029522‑M1/1‑23 (MQ=255)

                                                                            gAAATGATGATTACATCGCTGCgcgataaatcgccacaatttcttcgtgagttgcctggatcgggttagtaaagccacaggcatctttcaggg             >  2:551353‑M1/1‑22 (MQ=255)

                                                                            gAAATGATGATTACATCGCTGCgcgataaatcgccacaatttcttcgtgagttgcctggatcgggttagtaaagccacaggcatctttcaggg             >  2:2119108‑M1/1‑22 (MQ=255)

                                                                              aaTGATGATTACATCGCTGCgcgataaatcgccacaatttcttcgtgagttgcctggatcgggttagtaaagccacaggcatctttcagggca           >  2:1768796‑M1/1‑20 (MQ=255)

                                                                                tgatgaTTACATCGCTGCgcgataaatcgccacaatttcttcgtgagttgcctggatcgggttagtaaagccacaggcatctttcagggcatt         >  2:5885919‑M1/1‑18 (MQ=255)

                                                                                tgatgaTTACATCGCTGCgcgataaatcgccacaatttcttcgtgagttgcctggatcgggttagtaaagccacaggcatctttcagggcatt         >  2:1993818‑M1/1‑18 (MQ=255)

                                                                                 gatgatTACATCGCTGCgcgataaatcgccacaatttcttcgtgagttgcctggatcgggttagtaaagccacaggcatctttcagggcatta        >  2:5804731‑M1/1‑17 (MQ=255)

                                                                                 gatgatTACATCGCTGCgcgataaatcgccacaatttcttcgtgagttgcctggatcgggttagtaaagccacaggcatctttcagggcatta        <  2:1351633‑M1/93‑77 (MQ=255)

                                                                                   tgatTACATCGCTGCgcgataaatcgccacaatttcttcgtgagttgcctggatcgggttagtaaagccacaggcatctttcagggcattagt      >  2:3248236‑M1/1‑15 (MQ=255)

                                                                                    gatTACATCGCTGCgcgataaatcgccacaatttcttcgtgagttgcctggatcgggttagtaaagccacaggcatctttcagggcattagtc     <  1:5027859‑M1/93‑80 (MQ=255)

                                                                                      ttACATCGCTGCgcgataaatcgccacaatttcttcgtgagttgcctggatcgggttagtaaagccacaggcatctttcagggcattagtcgc   >  2:3673331‑M1/1‑12 (MQ=255)

                                                                                       tACATCGCTGCgcgataaatcgccacaatttcttcgtgagttgcctggatcgggttagtaaagccacaggcatctttcagggcattagtcgcc  <  2:4723529‑M1/93‑83 (MQ=255)

                                                                                       tACATCGCTGCgcgataaatcgccacaatttcttcgtgagttgcctggatcgggttagtaaagccacaggcatctttcagggcattagtcgca  >  2:1082635‑M1/1‑11 (MQ=255)

GACAGCTATCACGAATTTACGGGCAAGGGTTACAGAGATAGTTGAAAAAGCGGCTAACAATTTGCCAGCCGTTGTGGAAATGATGATTACCTCGCTGCATAAATCGCCACAATTTCTTCGTGAGTTGCCTGGATCGGGTTAGTAAAGCCACAGGCATCTTTCAGGGCATTAGTCGCCAAT  >  NC_000913/3754220‑3754399

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 20 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`