BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	481,041	A→G	S863P (TCC→CCC)	acrB ←	acridine efflux pump

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	481,041	0	A	G	99.5%	693.9 / NA	217	S863P (TCC→CCC)	acrB	acridine efflux pump
Reads supporting (aligned to +/- strand): ref base A (0/0); major base G (113/103); minor base C (1/0); total (114/103)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 219 total aligned reads displayed.

CGTACAGCGCCGCCAGACACAGGAACACGACAATCAACGAAATCGCGTACAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTGATAGGACATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGTTTGCTCGCCAGTTGTTCCATCAGCTCCATTGCTTCACCGGTACTTTTACCC  >  NC_000913/480951‑481129

 gTACAGCGCCGCCAGACACAGGAACACGACAATCAACGAAATCGCGTACAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTGATAGGGCAt                                                                                       <  2:5528802/93‑1 (MQ=255)

  tACAGCGCCGCCAGACACAGGAACACGACAATCAACGAAATCGCGTACAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTGATAGGGCATc                                                                                      >  1:4926824/1‑93 (MQ=255)

   aCAGCGCCGCCAGACACAGGAACACGACAATCAACGAAATCGCGTACAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTGATAGGGCATcc                                                                                     <  2:5680576/93‑1 (MQ=255)

    cAGCGCCGCCAGACACAGGAACACGACAATCAACGAAATCGCGTACAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTGATAGGGCATccc                                                                                    >  2:1824053/1‑93 (MQ=255)

    cAGCGCCGCCAGACACAGGAACACGACAATCAACGAAATCGCGTACAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTGATAGGGCATccc                                                                                    >  1:2663620/1‑93 (MQ=255)

     aGCGCCGCCAGACACAGGAACACGACAATCAACGAAATCGCGTACAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTGATAGGGCATcccc                                                                                   >  2:1170182/1‑93 (MQ=255)

      gcgcCGCCAGACACAGGAACACGACAATCAACGAAATCGCGTACAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTGATAGGCCATCCCCg                                                                                  >  2:2095992/1‑93 (MQ=255)

       cgccgcCAGACACAGGAACACGACAATCAACGAAATCGCGTACAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTGATAGGGCATCCCCGt                                                                                 <  2:2902656/93‑1 (MQ=255)

       cgccgcCAGACACAGGAACACGACAATCAACGAAATCGCGTACAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTGATAGGGCATCCCCGt                                                                                 <  2:2806474/93‑1 (MQ=255)

          cgccAGACACAGGAACACGACAATCAACGAAATCGCGTACAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTGCTAGGGCATCCCCGTccg                                                                              >  2:1200748/1‑92 (MQ=255)

          cgccAGACACAGGAACACGACAATCAACGAAATCGCGTACAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTGATAGGGCATCCCCGTCCa                                                                              >  1:474406/1‑93 (MQ=255)

           gccAGACACAGGAACACGACAATCAACGAAATCGCGTACAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTGATAGGGCATCCCCGTCCAg                                                                             <  1:6337253/93‑1 (MQ=255)

            ccAGACACAGGAACACGACAATCAACGAAATCGCGTACAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTGATAGGGCATCCCCGTCCAGt                                                                            >  2:5710976/1‑93 (MQ=255)

             cAGACACAGGAACACGACAATCAACGAAATCGCGTACAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTGATAGGGCATCCCCGTCCAGTc                                                                           >  1:949430/1‑93 (MQ=255)

             cAGACACAGGAACACGACAATCAACGAAATCGCGTACAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTGATAGGGCATCCCCGTCCAGTc                                                                           <  2:4794136/93‑1 (MQ=255)

               gACACAGGAACACGACAATCAACGAAATCGCGTACAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTGATAGGGCATCCCCGTCCAGTCAt                                                                         >  1:5265110/1‑93 (MQ=255)

                  acaGGAACACGACAATCAACGAAATCGCGTACAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTGATAGGGCATCCCCGTCCAGTCATCGc                                                                      >  2:1121145/1‑93 (MQ=255)

                   caGGAACACGACAATCAACGAAAACGCGTACAGTGAAGGTGCCTGGTTGCCGGGGAGACGTTCCTGATAGGGCATCCCCGTCCAGTCCTAgac                                                                     >  1:988686/1‑91 (MQ=255)

                     ggAACACGACAATCAACGAAATCGCGTACAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTGATAGGGCATCCCCGTCCAGTCATAGCCaa                                                                   >  1:5495978/1‑93 (MQ=255)

                     ggAACACGACAATCAACGAAATCGCGTACAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTGATAGGGCATCCCCGTCCAGTCATAGCCaa                                                                   >  1:6392736/1‑93 (MQ=255)

                      gAACACGACAATCAACGAAATCGCGTACAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTGATAGGGCATCCCCGTCCAGTCATAGCCAac                                                                  <  1:1021059/93‑1 (MQ=255)

                        acacGACAATCAACGAAATCGCGTACAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTGATAGGGCATCCCCGTCCAGTCATAGCCAacac                                                                >  2:4912355/1‑93 (MQ=255)

                        acacGACAATCAACGAAATCGCGTACAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTGATAGGGCATCCCCGTCCAGTCATAGCCAacac                                                                >  1:1944363/1‑93 (MQ=255)

                          acGACAATCAACGAAATCGCGTACAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTGATAGGGCATCCCCGTCCAGTCATAGCCAACACCg                                                              >  2:1609063/1‑93 (MQ=255)

                             aCAATCAACGAAATCGCGTACAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTGATAGGGCATCCCCGTCCAGTCATAGCCAACACCGGTa                                                           <  2:3477285/93‑1 (MQ=255)

                             aCAATCAACGAAATCGCGTACAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTGATAGGGCATCCCCGTCCAGTCATAGCCAACACCGGTa                                                           <  1:2433769/93‑1 (MQ=255)

                             aCAATCAACGAAATCGCGTACAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTGATAGGGCATCCCCGTCCAGTCATAGCCAACACCGGTa                                                           <  2:6205507/93‑1 (MQ=255)

                              cAATCAACGAAATCGCGTACAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTGATAGGGCATCCCCGTCCAGTCATAGCCAACACCGGTAg                                                          <  1:5689325/93‑1 (MQ=255)

                               aaTCAACGAAATCGCGTACAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTGATAGGGCATCCCCGTCCAGTCATAGCCAACACCGGTAgg                                                         >  1:4539534/1‑93 (MQ=255)

                               aaTCAACGAAATCGCGTACAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTGATAGGGCATCCCCGTCCAGTCATAGCCAACACCGGTAgg                                                         >  1:5957311/1‑93 (MQ=255)

                                aTCAACGAAATCGCGTACAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTGATAGGGCATCCCCGTCCAGTCATAGCCAACACCGGTAGGc                                                        <  2:1673842/93‑1 (MQ=255)

                                 tCAACGAAATCGCGTACAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTGATAGGGCATCCCCGTCCAGTCATAGCCAACACCGGTAGGCa                                                       >  1:2049123/1‑93 (MQ=255)

                                 tCAACGAAATCGCGTACAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTGATAGGGCATCCCCGTCCAGTCATAGCCAACACCGGTAGGCa                                                       <  2:3042141/93‑1 (MQ=255)

                                  cAACGAAATCGCGTACAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTGATAGGGCATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAg                                                      <  1:3405922/93‑1 (MQ=255)

                                   aaCGAAATCGCGTACAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTGATAGGGCATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGt                                                     <  2:153023/93‑1 (MQ=255)

                                   aaCGAAATCGCGTACAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTGATAGGGCATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGt                                                     <  1:4833342/93‑1 (MQ=255)

                                   aaCGAAATCGCGTACAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTGATAGGGCATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGt                                                     <  2:4937931/93‑1 (MQ=255)

                                    aCGAAATCGCGTACAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTGATAGGGCATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGtt                                                    <  1:3328579/93‑1 (MQ=255)

                                    aCGAAATCGCGTACAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTGATAGGGCATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGtt                                                    <  2:1277176/93‑1 (MQ=255)

                                    aCGAAATCGCGTACAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTGATAGGGCATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGtt                                                    >  2:6530770/1‑93 (MQ=255)

                                    aCGAAATCGCGTACAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTGATAGGGCATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGtt                                                    <  1:1161163/93‑1 (MQ=255)

                                    aCGAAATCGCGTACAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTGATAGGGCATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGtt                                                    >  1:1252202/1‑93 (MQ=255)

                                    aCGAAATCGCGTACAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTGATAGGGCATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGtt                                                    <  2:5986522/93‑1 (MQ=255)

                                    aCGAAATCGCGTACAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTGATAGGGCATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGtt                                                    <  1:3190201/93‑1 (MQ=255)

                                    aCGAAATCGCGTACAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTGATAGGGCATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGtt                                                    >  2:3856387/1‑93 (MQ=255)

                                    aCGAAATCGCGTACAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTGATAGGGCATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGtt                                                    <  1:5072887/93‑1 (MQ=255)

                                    aCGAAATCGCGTACAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTGATAGGGCATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGtt                                                    <  2:2966651/93‑1 (MQ=255)

                                       aaaTCGCGTACAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTGATAGGGCATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGTTTGc                                                 >  1:1666611/1‑93 (MQ=255)

                                        aaTCGCGTACAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTGATAGGGCATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGTTTGCt                                                >  1:411151/1‑93 (MQ=255)

                                         aTCGCGTACAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTGATAGGGCATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGTTTGCTc                                               >  2:3963802/1‑93 (MQ=255)

                                         aTCGCGTACAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTGATAGGGCATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGTTTGCTc                                               >  1:5819268/1‑93 (MQ=255)

                                            gcgTACAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTGATAGGGCATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGTTTGCTCGcc                                            <  2:2127510/93‑1 (MQ=255)

                                            gcgTACAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTGATAGGGCATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGTTTGCTCGcc                                            <  2:2878344/93‑1 (MQ=255)

                                            gcgTACAGGGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTGATAGGGCATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGTTTGCTCGcc                                            <  1:107479/93‑1 (MQ=255)

                                             cgTACAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTGATAGGGCATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGTTTGCTCGCCa                                           >  2:5643612/1‑93 (MQ=255)

                                              gTACAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTGATAGGGCATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGTTTGCTCGCCAg                                          >  1:171484/1‑93 (MQ=255)

                                              gTACAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTGATAGGGCATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGTTTGCTCGACAg                                          >  1:837622/1‑93 (MQ=255)

                                              gTACAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTGATAGGGCATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAATTTGCTCGCCAg                                          >  1:2612991/1‑93 (MQ=255)

                                               tACAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTGATAGGGCATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGTTTGCTCGCCAgt                                         <  2:1464498/93‑1 (MQ=255)

                                                aCAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTGATAGGGCATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGTTTGCTCGCCAgtt                                        >  2:4682520/1‑93 (MQ=255)

                                                  aGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTGATAGGGCATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGTTTGCTCGCCAgttgt                                      <  1:4369225/93‑1 (MQ=255)

                                                    tGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTGATAGGGCATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGTTTGCTCGCCAGTTGTTc                                    <  2:908918/93‑1 (MQ=255)

                                                      aaGGTGCCTGGTTGCCGGAGAGACGTTCCTGATAGGGCATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGTTTGCTCGCCAGTTGTTCCa                                  <  1:6172045/93‑1 (MQ=255)

                                                       aGGTGCCTGGTTGCCGGAGAGACGTTCCTGATAGGGCATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGTTTGCTCGCCAGTTGTTCCAt                                 >  2:2470003/1‑93 (MQ=255)

                                                       aGGTGCCTGGTTGCCGGAGAGACGTTCCTGATAGGGCATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGTTTGCTCGCCAGTTGTTCCAt                                 >  1:3671849/1‑93 (MQ=255)

                                                        ggTGCCTGGTTGCCGGAGAGACGTTCCTGATAGGGCATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGTTTGCTCGCCAGTTGTTCCATc                                <  1:6036476/93‑1 (MQ=255)

                                                         gTGCCTGGTTGCCGGAGAGACGTTCCTGATAGGGCATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGTTTGCTCGCCAGTTGTTCCATCa                               >  2:4460805/1‑93 (MQ=255)

                                                         gTGCCTGGTTGCCGGAGAGACGTTCCTGATAGGGCATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGTTTGCTCGCCAGTTGTTCCATCa                               <  2:3242028/93‑1 (MQ=255)

                                                            ccTGGTTGCCGGAGAGACGTTCCTGATAGGGCATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGTTTGCTCGCCAGTTGTTCCATCAGCt                            <  1:5868384/93‑1 (MQ=255)

                                                             cTGGTTGCCGGAGAGACGTTCCTGATAGGGCATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGTTTGCTCGCCAGTTGTTCCATCAGCTc                           >  1:2696145/1‑93 (MQ=255)

                                                             cTGGTTGCCGGAGAGACGTTCCTGATAGGGCATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGTTTGCTCGCCAGTTGTTCCATCAGCTc                           >  1:5790423/1‑93 (MQ=255)

                                                             cTGGTTGCCGGAGAGACGTTCCTAATAGGGCATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGTTTGCTCGCCAGTTGTTCCATCAGCTc                           <  1:3500032/93‑1 (MQ=255)

                                                              tGGTTGCCGGAGAGACGTTCCTGATAGGGCATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGTTTGCTCGCCAGTTGTTCCATCAGCTcc                          >  1:1287504/1‑93 (MQ=255)

                                                               ggTTGCCGGAGAGACGTTCCTGATAGGGCATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGTTTGCTCGCCAGTTGTTCCATCAGCTCCa                         <  2:4631541/93‑1 (MQ=255)

                                                               ggTTGCCGGAGAGACGTTCCTGATAGGGCATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGTTTGCTCGCCAGTTGTTCCATCAGCTCCa                         <  1:2857967/93‑1 (MQ=255)

                                                               ggTTGCCGGAGAGACGTTCCTGATAGGGCATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGTTTGCTCGCCAGTTGTTCCATCAGCTCCa                         <  2:401323/93‑1 (MQ=255)

                                                                gTTGCCGGAGAGACGTTCCTGATAGGGCATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGTTTGCTCGCCAGTTGTTCCATCAGCTCCAt                        <  2:3747626/93‑1 (MQ=255)

                                                                gTTGCCGGAGAGACGTTCCTGATAGGGCATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGTTTGCTCGCCAGTTGTTCCATCAGCTCCAt                        <  2:4364024/93‑1 (MQ=255)

                                                                gTTGCCGGAGAGACGTTCCTGATAGGGCATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGTTTGCTCGCCAGTTGTTCCATCAGCTCCAt                        >  2:2684969/1‑93 (MQ=255)

                                                                gTTGCCGGAGAGACGTTCCTGATAGGGCATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGTTTGCTCGCCAGTTGTTCCATCAGCTCCAt                        <  2:5409258/93‑1 (MQ=255)

                                                                gTTGCCGGAGAGACGTTCCTGATAGGGCATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGTTTGCTCGCCAGTTGTTCCATCAGCTCCAt                        <  2:2769503/93‑1 (MQ=255)

                                                                 ttGCCGGAGAGACGTTCCTGATAGGGCATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGTTTGCTCGCCAGTTGTTCCATCCGCTCCAtt                       >  1:6328753/1‑93 (MQ=255)

                                                                 ttGCCGGAGAGACGTTCCTGATAGGGCATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGTTTGCTCGCCAGTTGTTCCATCAGCTCCAtt                       >  2:2844399/1‑93 (MQ=255)

                                                                  tGCCGGAGAGACGTTCCTGATAGGGCATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGTTTGCTCGCCAGTTGTTCCATCATCTCCATTg                      <  1:6531486/93‑1 (MQ=255)

                                                                   gCCGGAGAGACGTTCCTGATAGGGCATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGTTTGCTCGCCAGTTGTTCCATCAGCTCCATTGc                     <  2:2338043/93‑1 (MQ=255)

                                                                    ccGGAGAGACGTTCCTGATAGGGCATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGTTTGCTCGCCAGTTGTTCCATCAGCTCCATTGCt                    >  1:280043/1‑93 (MQ=255)

                                                                    ccGGAGAGACGTTCCTGATAGGGCAACCCCGTCCAGTCATAGCCAACACCGGTAGGCAGTTTGCTCGCCAGTTGTTCCATCAGCTCCATTGCt                    <  1:6671606/93‑1 (MQ=255)

                                                                       gagagaCGTTCCTGATAGGGCATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGTTTGCTCGCCAGTTGTTCCATCAGCTCCATTGCTTCa                 <  1:6100372/93‑1 (MQ=255)

                                                                       gagagaCGTTCCTGATAGGGCATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGTTTGCTCGCCAGTTGTTCCATCAGCTCCATTGCTTCa                 <  2:4710683/93‑1 (MQ=255)

                                                                       gagagaCGTTCCTGATAGGGCATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGTTTGCTCGCCAGTTGTTCCATCAGCTCCATTGCTTCa                 >  1:1089210/1‑93 (MQ=255)

                                                                         gagaCGTTCCTGATAGGGCATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGTTTGCTCGCCAGTTGTTCCATCAGCTCCATTGCTTCAcc               >  2:5084690/1‑93 (MQ=255)

                                                                             cGTTCCTGATAGGGCATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGTTTGCTCGCCAGTTGTTCCATCAGCTCCATTGCTTCACCGGTa           <  1:4308140/93‑1 (MQ=255)

                                                                              gTTCCTGATAGGGCATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGTTTGCTCGCCAGTTGTTCCATCAGCTCCATTGCTTCACCGGTAc          <  1:3139375/93‑1 (MQ=255)

                                                                              gTTCCTGATAGGGCATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGTTTGCTCGCCAGTTGTTCCATCAGCTCCATTGCTTCACCGGTAc          <  1:5170038/93‑1 (MQ=255)

                                                                               ttCCTGATAGGGCATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGTTTGCTCGCCAGTTGTTCCATCAGCTCCATTGCTTCACCGGTACt         >  2:2304445/1‑93 (MQ=255)

                                                                                 ccTGATAGCGCATCCCCGTTCAGTCATAGCCAACACCGGTAGGCAGTTTGCTCGCCAGTTGTTCCATCAGCTCCATTGCTTCACCGGTACttt       >  1:6569951/1‑93 (MQ=255)

                                                                                  cTGATAGGGCATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGTTTGCTCGCCAGTTGTTCCATCAGCTCCATTGCTTCACCGGTACtttt      >  2:788961/1‑93 (MQ=255)

                                                                                     aTAGGGCATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGTTTGCTCGCCAGTTGTTCCATCAGCTCCATTGCTTCACCGGTACTTTTAcc   <  2:5265690/93‑1 (MQ=255)

                                                                                      tAGGGCATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGTTTGCTCGCCAGTTGTTCCATCAGCTCCATTGCTTCACCGGTACTTTTAccc  <  2:2627470/93‑1 (MQ=255)

                                                                                      tAGGGCATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGTTTGCTCGCCAGTTGTTCCATCAGCTCCATTGCTTCACCGGTACTTTTAccc  >  2:6350771/1‑93 (MQ=255)

CGTACAGCGCCGCCAGACACAGGAACACGACAATCAACGAAATCGCGTACAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTGATAGGACATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGTTTGCTCGCCAGTTGTTCCATCAGCTCCATTGCTTCACCGGTACTTTTACCC  >  NC_000913/480951‑481129

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 20 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`