Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,964,245 T→C Y72H (TAC→CAC)  rho → transcription termination factor Rho

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,964,2450TC99.5% 700.0 / NA 218Y72H (TAC→CAC) rhotranscription termination factor Rho
Reads supporting (aligned to +/- strand):  ref base T (0/0);  major base C (109/108);  minor base A (0/1);  total (109/109)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 231 total aligned reads displayed.

ACGCAAAGAGTGGCGAAGATATCTTTGGTGATGGCGTACTGGAGATATTGCAGGATGGATTTGGTTTCCTCCGTTCCGCAGACAGCTCCTACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCCAAATCCGCCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATT  >  NC_000913/3964156‑3964334
                                                                                         |                                                                                         
 cGCAAAGAGTGGCGAAGATATCTTTGGTGATGGCGTACTGGAGATATTGCAGGATGGATTTGGTTTCCTCCGTTCCGCAGACAGCTCCCACCt                                                                                       >  1:2643420/1‑93 (MQ=255)
   cAAAGAGTGGCGAAGATATCTTTGGTGATGGCGTACTGGAGATATTGCAGGATGGATTTGGTTTCCTCCGTTCCGCAGACAGCTCCCACCTCg                                                                                     <  2:5367218/93‑1 (MQ=255)
    aaaGAGTGGCGAAGATATCTTTGGTGATGGCGTACTGGAGATATTGCAGGATGGATTTGGTTTCCTCCGTTCCGCAGACAGCTCCCACCTCGc                                                                                    >  1:951327/1‑93 (MQ=255)
     aaGAGTGGCGAAGATATCTTTGGTGATGGCGTACTGGAGATATTGCAGGATGGATTTGGTTTCCTCCGTTCCGCAGACAGCTCCCACCTCGcc                                                                                   >  1:866274/1‑93 (MQ=255)
      agagTGGCGAAGATATCTTTGGTGATGGCGTACTGGAGATATTGCAGGATGGATTTGGTTTCCTCCGTTCCGCAGACAGCTCCCACCTCGCCg                                                                                  <  1:1072899/93‑1 (MQ=255)
      agagTGGCGAAGATATCTTTGGTGATGGCGTACTGGAGATATTGCAGGATGGATTTGGTTTCCTCCGTTCCGCAGACAGCTCCCACCTCGCCg                                                                                  <  1:6388758/93‑1 (MQ=255)
      agagGGGCGAAGATATCTTTGGTGATGGCGTACTGGAGATATTGCAGGAAGGATTTGGCTTCCTCCACTCCGCAGACAGCTCCCACCTCGCCg                                                                                  >  2:2432842/1‑93 (MQ=255)
         gTGGCGAAGATATCTTTGGTGATGGCGTACTGGAGATATTGCAGGATGGATTTGGTTTCCTCCGTTCCGCAGACAGCTCCCACCTCGCCGGTc                                                                               <  1:1125495/93‑1 (MQ=255)
         gTGGCGAAGATATCTTTGGTGATGGCGTACTGGAGATATTGCAGGATGGATTTGGTTTCCTCCGTTCCGCAGACAGCTCCCACCTCGCCGGTc                                                                               <  2:2308784/93‑1 (MQ=255)
         gTGGCGAAGATATCTTTGGTGATGGCGTACTGGAGATATTGCAGGATGGATTTGGTTTCCTCCGTTCCGCAGACAGCTCCCACCTCGCCGGTc                                                                               <  1:5560833/93‑1 (MQ=255)
         gTGGCGAAGATATCTTTGGTGATGGCGTACTGGAGATATTGCAGGATGGATTTGGATTCCTCCGTTCCGCAGACAGCTACCACCTCGCCAGTc                                                                               >  1:5889388/1‑93 (MQ=255)
           ggCGAAGATATCTTTGGTGATGGCGTACTGGAGATATTGCAGGATGGATTTGGTTTCCTCCGTTCCGCAGACAGCTCCCACCTCGCCGGTCCt                                                                             <  1:3364279/93‑1 (MQ=255)
           ggCGAAGATATCTTTGGTGATGGCGTACTGGAGATATTGCAGGATGGATTTGGTTTCCTCCGTTCCGCAGACAGCTCCCACCTCGCCGGTCCt                                                                             >  1:4028743/1‑93 (MQ=255)
           ggCGAAGATATCTTTGGTGATGGCGTACTGGAGATATTGCAGGATGGATTTGGTTTCCTCCGTTCCGCAGACAGCTCCCACCTCGCCGGTCCt                                                                             >  2:4461914/1‑93 (MQ=255)
           ggCGAAGATATCTTTGGTGATGGCGTACTGGAGATATTGCAGGATGGATTTGGTTTCCTCCGTTCCGCAGACAGCTCCCACCTCGCCGGTCCt                                                                             <  1:1566592/93‑1 (MQ=255)
            gCGAAGATATCTTTGGTGATGGCGTACTGGAGATATTGCAGGATGGATTTGGTTTCCTCCGTTCCGCAGACAGCTCCCACCTCGCCGGTCCtg                                                                            >  2:1691688/1‑93 (MQ=255)
             cGAAGATATCTTTGGTGATGGCGTACTGGAGATATTGCAGGATGGATTTGGTTTCCTCCGTTCCGCAGACAGCTCCCACCTCGCCGGTCCtga                                                                           <  1:3486374/93‑1 (MQ=255)
              gAAGATATCTTTGGTGATGGGGGACTGGAGATATTGCAGGATGGGTTTGGTTTCCTCCGTTCCGCAGACAGCTCCCACCTCGCCGGTCCtgat                                                                          >  1:2320153/1‑93 (MQ=255)
              gAAGATATCTTTGGTGATGGCGTACTGGAGATATTGCAGGATGGATTTGGTTTCCTCCGTTCCGCAGACAGCTCCCACCTCGCCGGTCCtgat                                                                          <  2:6491526/93‑1 (MQ=255)
               aaGATATCTTTGGTGATGGCGTACTGGAGATATTGCAGGATGGATTTGGTTTCCTCCGTTCCGCAGACAGCTCCCACCTCGCCGGTCCtgatg                                                                         >  2:1725333/1‑93 (MQ=255)
               aaGATATCTTTGGTGATGGCGTACTGGAGATATTGCAGGATGGATTTGGTTTCCTCCGTTCCGCAGACAGCTCCCACCTCGCCGGTCCtgatg                                                                         >  2:4353950/1‑93 (MQ=255)
                aGATATCTTTGGTGATGGCGTACTGGAGATATTGCAGGATGGATTTGGTTTCCTCCGTTCCGCAGACAGCTCCCACCTCGCCGGTCCtgatga                                                                        <  2:1454469/93‑1 (MQ=255)
                aGATATCTTTGGTGATGGCGTACTGGAGATATTGCAGGATGGATTTGGTTTCCTCCGTTCCGCAGACAGCTCCCACCTCGCCGGTCCtgatga                                                                        >  1:4047511/1‑93 (MQ=255)
                 gATATCTTTGGTGATGGCGTACTGGAGATATTGCAGGATGGATTTGGTTTCCTCCGTTCCGCAGACAGCTCCCACCTCGCCGGTCCTGATGAc                                                                       <  1:3421081/93‑1 (MQ=255)
                 gATATCTTTGGGGATGGCGTACTGGAGATATTGCAGGATGGATTTGGTTTCCTCCGTTCCGCAGACAGCTCCCACCTCGCCGGTCCTGATGAc                                                                       >  2:2375644/1‑93 (MQ=255)
                    atCTTTGGTGATGGCGTACTGGAGATATTGCAGGATGGATTTGGTTTCCTCCGTTCCGCAGACAGCTCCCACCTCGCCGGTCCTGATGACATc                                                                    >  2:111986/1‑93 (MQ=255)
                    atCTTTGGTGATGGCGTACTGGAGATATTGCAGGATGGATTTGGTTTCCTCCGTTCCGCAGACAGCTCCCACCTCGCCGGTCCTGATGACATc                                                                    <  1:1850485/93‑1 (MQ=255)
                     tCTTTGGTGATGGCGTACTGGAGATATTGCAGGATGGATTTGGTTTCCTCCGTTCCGCAGACAGCTCCCACCTCGCCGGTCCTGATGACATCt                                                                   <  2:4745186/93‑1 (MQ=255)
                     tCTTTGGTGATGGCGTACTGGAGATATTGCAGGATGGATTTGGTTTCCTCCGTTCCGCAGACAGCTCCCACCTCGCCGGTCCTGATGACATCt                                                                   >  2:1510332/1‑93 (MQ=255)
                     tCTTTGGTGATGGCGTACTGGAGATATTGCAGGATGGATTTGGTTTCCTCCGTTCCGCAGACAGCTCCCACCTCGCCGGTCCTGATGACATCt                                                                   >  2:2135401/1‑93 (MQ=255)
                       tttGGTGATGGCGTACTGGAGATATTGCAGGATGGATTTGGTTTCCTCCGTTCCGCAGACAGCTCCCACCTCGCCGGTCCTGATGACATCTAc                                                                 >  2:4029541/1‑93 (MQ=255)
                           gTGATGGCGTACTGGAGATATTGCAGGATGGATTTGGTTTCCTCCGTTCCGCAGACAGCTCCCACCTCGCCGGTCCTGATGACATCTACGttt                                                             <  1:2787643/93‑1 (MQ=255)
                           gTGATGGCGTACTGGAGATATTGCAGGATGGATTTGGTTTCCTCCGTTCCGCAGACAGCTCCCACCTCGCCGGTCCTGATGACATCTACGttt                                                             >  1:4939947/1‑93 (MQ=255)
                            tGATGGCGTACTGGAGATATTGCAGGATGGATTTGGTTTCCTCCGTTCCGCAGACAGCTCCCACCTCGCCGGTCCTGATGACATCTACGTTTc                                                            <  2:2578217/93‑1 (MQ=255)
                              aTGGCGTACTGGAGATATTGCAGGATGGATTTGGTTTCCTCCGTTCCGCAGACAGCTCCCACCTCGCCGGTCCTGATGACATCTACGATTccc                                                          >  1:6726208/1‑93 (MQ=255)
                               tGGCGTACTGGAGATATTGCAGGATGGATTTGGTTTCCTCCGTTCCGCAGACAGCTCCCACCTCGCCGGTCCTGATGACATCTACGTTTcccc                                                         <  1:2415360/93‑1 (MQ=255)
                               tGGCGTACTGGAGATATTGCAGGATGGATTTGGTTTCCTCCGTTCCGCAGACAGCTCCCACCTCGCCGGTCCTGATGACATCTACGTTTcccc                                                         <  1:3344317/93‑1 (MQ=255)
                                ggCGTACTGGAGATATTGCAGGATGGATTTGGTTTCCTCCGTTCCGCAGACAGCTCCCACCTCGCCGGTCCTGATGACATCTACGTTTCCCCt                                                        <  1:5118572/93‑1 (MQ=255)
                                    tACTGGAGATATTGCAGGATGGATTTGGTTTCCTCCGTTCCGCAGACAGCTCCCACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGcc                                                    >  2:2625646/1‑93 (MQ=255)
                                    tACTGGAGATATTGCAGGATGGATTTGGTTTCCTCCGTTCCGCAGACAGCTCCCACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGcc                                                    >  2:2885378/1‑93 (MQ=255)
                                    tACTGGAGATATTGCAGGATGGATTTGGTTTCCTCCGTTCCGCAGACAGCTCCCACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGcc                                                    >  1:1710234/1‑93 (MQ=255)
                                     aCTGGAGATATTGCAGGATGGATTTGGTTTCCTCCGTTCCGCAGACAGCTCCCACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCCa                                                   >  2:3008397/1‑93 (MQ=255)
                                       tGGAGATATTGCAGGATGGATTTGGTTTCCTCCGTTCCGCAGACAGCTCCCACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCCaaa                                                 >  1:2993021/1‑93 (MQ=255)
                                       tGGAGATATTGCAGGATGGATTTGGTTTCCTCCGTTCCGCAGACAGCTCCCACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCCaaa                                                 >  2:2826341/1‑93 (MQ=255)
                                       tGGAGATATTGCAGGATGGATTTGGTTTCCTCCGTTCCGCAGACAGCTCCCACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCCaaa                                                 >  1:1506517/1‑93 (MQ=255)
                                        ggAGATATTGCAGGATGGATTTGGTTTCCTCCGTTCCGCAGACAGCTCCCACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCCAAAt                                                >  2:5208295/1‑93 (MQ=255)
                                         gagaTATTGCAGGATGGATTTGGTTTCCTCCGTTCCGCAGACAGCTCCCACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCCAAATc                                               >  2:3378237/1‑93 (MQ=255)
                                          agaTATTGCAGGATGGATTTGGTTTCCTCCGTTCCGCAGACAGCTCCCACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCCAAATcc                                              <  1:3122560/93‑1 (MQ=255)
                                           gaTATTGCAGGATGGATTTGGTTTCCTCCGTTCCGCAGACAGCTCCCACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCCAAATccg                                             >  1:2739592/1‑93 (MQ=255)
                                           gaTATTGCAGGATGGATTTGGTTTCCTCCGTTCCGCAGACAGCTCCCACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCCAAATccg                                             <  1:6069964/93‑1 (MQ=255)
                                            atatTGCAGGATGGATTTGGTTTCCTCCGTTCCGCAGACAGCTCCCACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCCAAATccgc                                            <  1:6681776/93‑1 (MQ=255)
                                            atatTGCAGGATGGATTTGGTTTCCTCCGTTCCGCAGACAGCTCCCACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCCAAATccgc                                            <  1:3211047/93‑1 (MQ=255)
                                             tatTGCAGGATGGATTTGGTTTCCTCCGTTCCGCAGACAGCTCCCACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCCAAATccgcc                                           <  2:5485850/93‑1 (MQ=255)
                                             tatTGCAGGATGGATTTGGTTTCCTCCGTTCCGCAGACAGCTCCCACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCCAAATccgcc                                           >  1:1026211/1‑93 (MQ=255)
                                             tatTGCAGGATGGATTTGGTTTCCTCCGTTCCGCAGACAGCTCCCACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCCAAATccgcc                                           >  2:5938764/1‑93 (MQ=255)
                                              atTGCAGGATGGATTTGGTTTCCTCCGTTCCGCAGACAGCTCCCACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCCAAATccgccg                                          <  1:4698526/93‑1 (MQ=255)
                                              atTGCAGGATGGATTTGGTTTCCTCCGTTCCGCAGACAGCTCCCACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCCAAATccgccg                                          >  2:4185200/1‑93 (MQ=255)
                                               ttGCAGGATGGATTTGGTTTCCTCCGTTCCGCAGACAGCTCCCACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCCAAATCCGCCGt                                         >  2:5129432/1‑93 (MQ=255)
                                                tGCAGGATGGATTTGGTTTCCTCCGTTCCGCAGACAGCTCCCACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCCAAATCCGCCGtt                                        <  1:3933652/93‑1 (MQ=255)
                                                 gCAGGATGGATTTGGTTTCCTCCGTTCCGCAGACAGCTCCCACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCCAAATCCGCCGttt                                       <  2:4916882/93‑1 (MQ=255)
                                                  cAGGATGGATTTGGTTTCCTCCGTTCCGCAGACAGCTCCCACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCCAAATCCGCCGTTTc                                      <  2:6283725/93‑1 (MQ=255)
                                                   aGGATGGATTTGGTTTCCTCCGTTCCGCAGACAGCTCCCACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCCAAATCCGCCGTTTCa                                     >  1:1871692/1‑93 (MQ=255)
                                                    ggatggatTTGGTTTCCTCCGTTCCGCAGACAGCTCCCACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCCAAATCCGCCGTTTCaa                                    >  2:3511087/1‑93 (MQ=255)
                                                    ggatggatTTGGTTTCCTCCGTTCCGCAGACAGCTCCCACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCCAAATCCGCCGTTTCaa                                    >  2:2748000/1‑93 (MQ=255)
                                                    ggatggatTTGGTTTCCTCCGTTCCGCAGACAGCTCCCACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCCAAATCCGCCGTTTCaa                                    >  1:2215203/1‑93 (MQ=255)
                                                     gatggatTTGGTTTCCTCCGTTCCGCAGACAGCTCCCACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCCAAATCCGCCGTTTCAAc                                   >  1:5978055/1‑93 (MQ=255)
                                                      gtggatTTGGTTTCCTCCGTTCCGCAGACAGCTCCCACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCCAAATCCGCCGTTTCAAcc                                  <  1:5654523/92‑1 (MQ=255)
                                                      atggatTTGGTTTCCTCCGTTCCGCAGACAGCTCCCACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCCAAATCCGCCGTTTCAAcc                                  <  2:3131167/93‑1 (MQ=255)
                                                        ggatTTGGTTTCCTCCGTTCCGCAGACAGCTCCCACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCCAAATCCGCCGTTTCAACCTc                                <  2:6160555/93‑1 (MQ=255)
                                                        ggatTTGGTTTCCTCCGTTCCGCAGACAGCTCCCACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCCAAATCCGCCGTTTCAACCTc                                <  2:4876303/93‑1 (MQ=255)
                                                          atTTGGTTTCCTCCGTTCCGCAGACAGCTCCCACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCCAAATCCGCCGTTTCAACCTCCg                              >  1:3861628/1‑93 (MQ=255)
                                                           tttGGTTTCCTCCGTTCCGCAGACAGCTCCCACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCCAAATCCGCCGTTTCAACCTCCGc                             >  1:5505910/1‑93 (MQ=255)
                                                            ttGGTTTCCTCCGTTCCGCAGACAGCTCCCACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCCAAATCCGCCGTTTCAACCTCCGCa                            <  1:6287468/93‑1 (MQ=255)
                                                             tGGTTCCCGCCCTTCCGCAGACAGCTCCCACCTCGCCGGTCCTGATGACATCCACGTTTCCCCTAGCCAAATCCGCCGTTTCAACCTCCGCAc                           <  1:6051146/93‑1 (MQ=255)
                                                              ggTTTCCTCCGTTCCGCAGACAGCTCCCACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCCAAATCCGCCGTTTCAACCTCCGCACt                          >  2:5710335/1‑93 (MQ=255)
                                                               gTTTCCTCCGTTCCGCAGACAGCTCCCACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCCAAATCCGCCGTTTCAACCTCCGCACTg                         >  1:6520918/1‑93 (MQ=255)
                                                                    ctccGTTCCGCAGACAGCTCCCACCTCGCCGGTCCTGATGACATCTACGCTTCCCCTAGCCAAATCCGCCGTTTCAACCTCCGCACTGGTGAt                    >  1:5341776/1‑93 (MQ=255)
                                                                     tccGTTCCGCAGACAGCTCCCACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCCAAATCCGCCGTTTCAACCTCCGCACTGGTGATa                   >  1:778283/1‑93 (MQ=255)
                                                                     tccGTTCCGCAGACAGCTCCCACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCCAAATCCGCCGTTTCAACCTCCGCACTGGTGATa                   <  2:5991104/93‑1 (MQ=255)
                                                                     tccGTTCCGCAGACAGCTCCCACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCCAAATCCGCCGTTTCAACCTCCGCACTGGTGATa                   >  1:5702349/1‑93 (MQ=255)
                                                                     tccGTTCCGCAGACAGCTCCCACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCCAAATCCGCCGTTTCAACCTCCGCACTGGTGATa                   >  2:1335623/1‑93 (MQ=255)
                                                                      ccGTTCCGCAGACAGCTCCCACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCCAAATCCGCCGTTTCAACCTCCGCACTGGTGATAc                  <  2:3673293/93‑1 (MQ=255)
                                                                       cGTTCCGCAGACAGCTCCCACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCCAAATCCGCCGTTTCAACCTCCGCACTGGTGATAcc                 >  1:4542019/1‑93 (MQ=255)
                                                                       cGTTCCGCAGACAGCTCCCACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCCAAATCCGCCGTTTCAACCTCCGCACTGGTGATAcc                 <  2:2060741/93‑1 (MQ=255)
                                                                          tCCGCAGACAGCTCCCACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCCAAATCCGCCGTTTCAACCTCCGCACTGGTGATACCAtc              <  1:1347307/93‑1 (MQ=255)
                                                                          tCCGCAGACAGCTCCCACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCCAAATCCGCCGTTTCAACCTCCGCACTGGTGATACCAtc              <  1:2866402/93‑1 (MQ=255)
                                                                          tCCGCAGACAGCTCCCACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCCAAATCCGCCGTTTCAACCTCCGCACTGGTGATACCAtc              <  1:3732201/93‑1 (MQ=255)
                                                                           ccGCAGACAGCTCCCACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCCAAATCCGCCGTTTCAACCTCCGCACTGGTGATACCAtct             <  1:1368477/93‑1 (MQ=255)
                                                                            cGCAGACAGCTCCCACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCCAAATCCGCCGTTTCAACCTCCGCACTGGTGATACCAtctc            >  2:2232470/1‑93 (MQ=255)
                                                                            cGCAGACAGCTCCCACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCCAAATCCGCCGTTTCAACCTCCGCACTGGTGATACCAtctc            >  1:1193145/1‑93 (MQ=255)
                                                                            cGCAGACAGCTCCCACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCCAAATCCGCCGTTTCAACCTCCGCACTGGTGATACCAtctc            <  2:72756/93‑1 (MQ=255)
                                                                               aGACAGCTCCCACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCCAAATCCGCCGTTTCAACCTCCGCACTGGTGATACCATCTCTgg         <  2:4814817/93‑1 (MQ=255)
                                                                                gACAGCTCCCACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCCAAATCCGCCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGt        >  1:1968564/1‑93 (MQ=255)
                                                                                gACAGCTCCCACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCCAAATCCGCCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGt        <  1:616416/93‑1 (MQ=255)
                                                                                  cAGCTCCCACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCCAAATCCGCCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTaa      <  2:5152567/93‑1 (MQ=255)
                                                                                  cAGCTCCCACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCCAAATCCGCCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTaa      >  2:1556397/1‑93 (MQ=255)
                                                                                    gCTCCCACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCCAAATCCGCCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGa    <  2:3421138/93‑1 (MQ=255)
                                                                                     cTCCCACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCCAAATCCGCCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGAt   <  2:4317218/93‑1 (MQ=255)
                                                                                     cTCCCACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCCAAATCCGCCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGAt   <  1:5453781/93‑1 (MQ=255)
                                                                                      tCCCACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCCAAATCCGCCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGAtt  >  1:1275230/1‑93 (MQ=255)
                                                                                         |                                                                                         
ACGCAAAGAGTGGCGAAGATATCTTTGGTGATGGCGTACTGGAGATATTGCAGGATGGATTTGGTTTCCTCCGTTCCGCAGACAGCTCCTACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCCAAATCCGCCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATT  >  NC_000913/3964156‑3964334

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑