BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,965,229	A→G	I400V (ATT→GTT)	rho →	transcription termination factor Rho

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,965,229	0	A	G	100.0%	975.7 / NA	294	I400V (ATT→GTT)	rho	transcription termination factor Rho
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (170/124); total (170/124)

Only 100 of 296 total aligned reads displayed.

CTGCTCACGACTCAGGAAGAACTGCAGAAAATGTGGATCCTGCGCAAAATCATTCACCCGATGGGCGAAATCGATGCAATGGAATTCCTCATTAATAAACTGGCAATGACCAAGACCAATGACGATTTCTTCGAAATGATGAAACGCTCATAAATTTGTCTTATGCCAAAAACGCCACGT  >  NC_000913/3965139‑3965318

ctgctCACGACTCAGGAAGAACTGCAGAAAATGTGGATCCTGCGCAAAATCATTCACCCGATGGGCGAAATCGATGCAATGGAATTCCTCGtt                                                                                         <  1:892998/93‑1 (MQ=255)

 tgctCACGACTCAGGAAGAACTGCAGAAAATGTGGATCCTGCGCAAAATCATTCACCCGATGGGCGAAATCGATGCAATGGAATTCCTCGTta                                                                                        >  2:6253494/1‑93 (MQ=255)

  gctCACGACTCAGGAAGAACTGCAGAAAATGTGGATCCTGCGCAAAATCATTCACCCGATGGGCGAAATCGATGCAATGGAATTCCTCGTtaa                                                                                       >  1:5920727/1‑93 (MQ=255)

   ctCACGACTCAGGAAGAACTGCAGAAAATGTGGATCCTGCGCAAAATCATTCACCCGATGGGCGAAATCGATGCAATGGAATTCCTCGTtaat                                                                                      >  1:4813912/1‑93 (MQ=255)

   ctCACGACTCAGGAAGAACTGCAGAAAATGTGGATCCTGCGCAAAATCATTCACCCGATGGGCGAAATCGATGCAATGGAATTCCTCGTtaat                                                                                      <  1:6102923/93‑1 (MQ=255)

   ctCACGACTCAGGAAGAACTGCAGAAAATGTGGATCCTGCGCAAAATCATTCACCCGATGGGCGAAATCGATGCAATGGAATTCCTCGTtaat                                                                                      >  2:5794206/1‑93 (MQ=255)

    tCACGACTCAGGAAGAACTGCAGAAAATGTGGATCCTGCGCAAAATCATTCACCCGATGGGCGAAATCGATGCAATGGAATTCCTCGTtaata                                                                                     <  2:3183430/93‑1 (MQ=255)

    tCACGACTCAGGAAGAACTGCAGAAAATGTGGATCCTGCGCAAAATCATTCACCCGATGGGCGAAATCGATGCAATGGAATTCCTCGTtaata                                                                                     >  2:5637395/1‑93 (MQ=255)

    tCACGACTCAGGAAGAACTGCAGAAAATGTGGATCCTGCGCAAAATCATTCACCCGATGGGCGAAATCGATGCAATGGAATTCCTCGTtaata                                                                                     <  2:666598/93‑1 (MQ=255)

       cGACTCAGGAAGAACTGCAGAAAATGTGGATCCTGCGCAAAATCATTCACCCGATGGGCGAAATCGATGCAATGGAATTCCTCGTTAATAAAc                                                                                  <  1:4905450/93‑1 (MQ=255)

        gACTCAGGAAGAAATGCAGAAAATGTGGATCCTGCGCAAAATCATTCACCCGATGGGCGAAATCGATGCAATGGAATTCCTCGTTAATAAACt                                                                                 >  1:6437684/1‑93 (MQ=255)

         aCTCAGGAAGAACTGCAGAAAATGTGGATCCTGCGCAAAATCATTCACCCGATGGGCGAAATCGATGCAATGGAATTCCTCGTTAATAAACTg                                                                                >  2:6562725/1‑93 (MQ=255)

           tCAGGAAGAACTGCAGAAAATGTGGATCCTGCGCAAAATCATTCACCCGATGGGCGAAATCGATGCAATGGAATTCCTCGTTAATAAACTGGc                                                                              >  2:2471465/1‑93 (MQ=255)

            cAGGAAGAACTGCAGAAAATGTGGATCCTGCGCAAAATCATTCACCCGATGGGCGAAATCGATGCAATGGAATTCCTCGTTAATAAACTGGCa                                                                             <  1:6350309/93‑1 (MQ=255)

              ggAAGAACTGCAGAAAATGTGGATCCTGCGCAAAATCATTCACCCGATGGGCGAAATCGATGCAATGGAATTCCTCGTTAATAAACTGGCAAt                                                                           >  2:515036/1‑93 (MQ=255)

              ggAAGAACTGCAGAAAATGTGGATCCTGCGCAAAATCATTCACCCGATGGGCGAAATCGATGCAATGGAATTCCTCGTTAATAAACTGGCAAt                                                                           >  2:3675954/1‑93 (MQ=255)

              ggAAGAACTGCAGAAAATGTGGATCCTGCGCAAAATCATTCACCCGATGGGCGAAATCGATGCAATGGAATTCCTCGTTAATAAACTGGCAAt                                                                           >  1:5334596/1‑93 (MQ=255)

              ggAAGAACTGCAGAAAATGTGGATCCTGCGCAAAATCATTCACCCGATGGGCGAAATCGATGCAATGGAATTCCTCGTTAATAAACTGGCAAt                                                                           <  2:790375/93‑1 (MQ=255)

               gaagaaCTGCAGAAAATGTGGATCCTGCGCAAAATCATTCACCCGATGGGCGAAATCGATGCAATGGAATTCCTCGTTAATAAACTGGCAATg                                                                          >  1:4078154/1‑93 (MQ=255)

                  gaaCTGCAGAAAATGTGGATCCTGCGCAAAATCATTCACCCGATGGGCGAAATCGATGCAATGGAATTCCTCGTTAATAAACTGGCAATgacc                                                                       >  1:3276366/1‑93 (MQ=255)

                  gaaCTGCAGAAAATGTGGATCCTGCGCAAAATCATTCACCCGATGGGCGAAATCGATGCAATGGAATTCCTCGTTAATAAACTGGCAATgacc                                                                       >  2:2582333/1‑93 (MQ=255)

                  gaaCTGCAGAAAATGTGGATCCTGCGCAAAATCATTCACCCGATGGGCGAAATCGATGCAATGGAATTCCTCGTTAATAAACTGGCAATgaca                                                                       >  1:2535221/1‑92 (MQ=255)

                   aaCTGCAGAAAATGTGGATCCTGCGCAAAATCATTCACCCGATGGGCGAAATCGATGCAATGGAATTCCTCGTTAATAAACTGGCAATgacca                                                                      >  2:5235194/1‑93 (MQ=255)

                    attGCAGAAAATGTGGATCCTGCGCAAAATCATTCACCCGATGGGCGAAATCGATGCAATGGAATTCCTCGTTAATAAACTGGCAATgaccaa                                                                     <  1:496684/91‑1 (MQ=255)

                    aCTGCAGAAAATGTGGATCCTGCGCAAAATCATTCACCCGATGGGCGAAATCGATGCAATGGAATTCCTCGTTAATAAACTGGCAATgaccaa                                                                     <  2:5771856/93‑1 (MQ=255)

                    aCTGCAGAAAATGTGGATCCTGCGCAAAATCATTCACCCGATGGGCGAAATCGATGCAATGGAATTCCTCGTTAATAAACTGGCAATgaccaa                                                                     >  2:3327273/1‑93 (MQ=255)

                     cTGCAGAAAATGTGGATCCTGCGCAAAATCATTCACCCGATGGGCGAAATCGATGCAATGGAATTCCTCGTTAATAAACTGGCAATgaccaag                                                                    >  1:313035/1‑93 (MQ=255)

                      tGCAGAAAATGTGGATCCTGCGCAAAATCATTCACCCGATGGGCGAAATCGATGCAATGGAATTCCTCGTTAATAAACTGGCAATgaccaaga                                                                   >  1:464366/1‑93 (MQ=255)

                      tGCAGAAAATGTGGATCCTGCGCAAAATCATTCACCCGATGGGCGAAATCGATGCAATGGAATTCCTCGTTAATAAACTGGCAATgaccaaga                                                                   >  2:2753017/1‑93 (MQ=255)

                       gCAGAAAATGTGGATCCTGCGCAAAATCATTCACCCGATGGGCGAAATCGATGCAATGGAATTCCTCGTTAATAAACTGGCAATgaccaagac                                                                  >  2:3466352/1‑93 (MQ=255)

                       gCAAAAAATGTGGATCCTGCGCAAAATCATTCACCCGATGGGCGAAATCGATGCAATGGAATTCCTCGTTAATAAACTGGCAATgaccaagac                                                                  <  2:1603190/93‑1 (MQ=255)

                        cAGAAAATGTGGATCCTGCGCAAAATCATTCACCAGATGGGCGAAATCGATGCAATGGAATTCCTCGTTAATAAACTGGCAATgaccaagacc                                                                 >  1:6212700/1‑93 (MQ=255)

                         aGAAAATGTGGATCCTGCGCAAAATCATTCACCCGATGGGCGAAATCGATGCAATGGAATTCCTCGTTAATAAACTGGCAATgaccaagacca                                                                >  1:4582505/1‑93 (MQ=255)

                         aGAAAATGTGGATCCTGCGCAAAATCATTCACCCGATGGGCGAAATCGATGCAATGGAATTCCTCGTTAATAAACTGGCAATgaccaagacca                                                                >  1:3590023/1‑93 (MQ=255)

                          gAAAATGTGGATCCTGCGCAAAATCATTCACCCGATGGGCGAAATCGATGCAATGGAATTCCTCGTTAATAAACTGGCAATgaccaagaccaa                                                               <  1:1291561/93‑1 (MQ=255)

                           aaaaTGTGGATCCTGCGCAAAATCATTCACCCGATGGGCGAAATCGATGCAATGGAATTCCTCGTTAATAAACTGGCAATGACCAAGACCAAt                                                              >  2:3984279/1‑93 (MQ=255)

                            aaaTGTGGATCCTGCGCAAAATCATTCACCCGATGGGCGAAATCGATGCAATGGAATTCCTCGTTAATAAACTGGCAATGACCAAGACCAATg                                                             <  1:525150/93‑1 (MQ=255)

                             aaTGTGGATCCTGCGCAAAATCATTCACCCGATGGGCGAAATCGATGCAATGGAATTCCTCGTTAATAAACTGGCAATGACCAAGACCAATGa                                                            >  1:6723911/1‑93 (MQ=255)

                               tgtgGATCCTGCGCAAAATCATTCACCCGATGGGCGAAATCGATGCAATGGAATTCCTCGTTAATAAACTGGCAATGACCAAGACCAATGACg                                                          <  1:5749413/93‑1 (MQ=255)

                                gtgGATCCTGCGCAAAATCATTCACCCGATGGGCGAAATCGATGCAATGGAATTCCTCGTTAATAAACTGGCAATGACCAAGACCAATGACGa                                                         <  2:1829325/93‑1 (MQ=255)

                                gtgGATCCTGCGCAAAATCATTCACCCGATGGGCGAAATCGATGCAATGGAATTCCTCGTTAATAAACTGGCAATGACCAAGACCAATGACGa                                                         >  2:4670728/1‑93 (MQ=255)

                                 tgGATCCTGCCCAAAACAATTCACCCGATGCGCGAAATCGATGCAATGGAATTCCTGGTTAATAAACTGGCAATGACCAAGACCAATGCCGAt                                                        <  1:6047267/93‑1 (MQ=255)

                                  ggATCCTGCGCAAAATCATTCACCCGATGGGCGAAATCGATGCAATGGAATTCCTCGTTAATAAACTGGCAATGACCAAGACCAATGACGAtt                                                       >  1:1077715/1‑93 (MQ=255)

                                    aTCCTGCGCAAAATCATTCACCCGATGGGCGAAATCGATGCAATGGAATTCCTCGTTAATAAACTGGCAATGACCAAGACCAATGACGATttc                                                     >  2:3421024/1‑93 (MQ=255)

                                    aTCCTGCGCAAAATCATTCACCCGATGGGCGAAATCGATGCAATGGAATTCCTCGTTAATAAACTGGCAATGACCAAGACCAATGACGATttc                                                     >  2:4534243/1‑93 (MQ=255)

                                    aTCCTGCGCAAAATCATTCACCCGATGGGCGAAATCGATGCAATGGAATTCCTCGTTAATAAACTGGCAATGACCAAGACCAATGACGATttc                                                     >  1:918996/1‑93 (MQ=255)

                                      ccTGCGCAAAATCATTCACCCGATGGGCGAAATCGATGCAATGGAATTCCTCGTTAATAAACTGGCAATGACCAAGACCAATGACGATttctt                                                   >  2:1994843/1‑93 (MQ=255)

                                       ccgcgcAAAATCATTCACCCGATGGGCGAAATCGATGCAATGGAATTCCTCGTTAATAAACTGGCAATGACCAAGACCAATGACGATttcttc                                                  <  2:862104/91‑1 (MQ=255)

                                       cTGCGCAAAATCATTCACCCGATGGGCGAAATCGATGCAATGGAATTCCTCGTTAATAAACTGGCAATGACCAAGACCAATGACGATttcttc                                                  >  1:1191937/1‑93 (MQ=255)

                                       cTGCGCAAAATCATTCACCCGATGGGCGAAATCGATGCAATGGAATTCCTCGTTAATAAACTGGCAATGACCAAGACCAATGACGATttcttc                                                  <  1:1466334/93‑1 (MQ=255)

                                        tGCGCAAAATCATTCACCCGATGGGCGAAATCGATGCAATGGAATTCCTCGTTAATAAACTGGCAATGACCAAGACCAATGACGATTTCTTCg                                                 >  1:5792832/1‑93 (MQ=255)

                                         gcgcAAAATCATTCACCCGATGGGCGAAATCGATGCAATGGAATTCCTCGTTAATAAACTGGCAATGACCAAGACCAATGACGATTTCTTCGa                                                >  1:5012253/1‑93 (MQ=255)

                                         gcgcAAAATCATTCACCCGATGGGCGAAATCGATGCAATGGAATTCCTCGTTAATAAACTGGCAATGACCAAGACCAATGACGATTTCTTCGa                                                >  2:1185308/1‑93 (MQ=255)

                                          cgcAAAATCATTCACCCGATGGGCGAAATCGATGCAATGGAATTCCTCGTTAATAAACTGGCAATGACCAAGACCAATGACGATTTCTTCGaa                                               >  2:228275/1‑93 (MQ=255)

                                            cAAAATCATTCACCCGATGGGCGAAATCGATGCAATGGAATTCCTCGTTAATAAACTGGCAATGACCAAGACCAATGACGATTTCTTCGAAat                                             <  1:3887047/93‑1 (MQ=255)

                                            cAAAATCATTCACCCGATGGGCGAAATCGATGCAATGGAATTCCTCGTTAATAAACTGGCAATGACCAAGACCAATGACGATTTCTTCGAAat                                             <  1:5495418/93‑1 (MQ=255)

                                            cAAAATCATTCACCCGATGGGCGAAATCGATGCAATGGAATTCCTCGTTAATAAACTGGCAATGACCAAGACCAATGACGATTTCTTCGAAat                                             <  1:5426661/93‑1 (MQ=255)

                                            cAAAATCATTCACCCGATGGGCGAAATCGATGCAATGGAATCCCTCGTTAATAAACTGGCAATGACCAAGACCAATGACGATTTCTTCGAAat                                             <  2:4101099/93‑1 (MQ=255)

                                               aaTCATTCACCCGATGGGCGAAATCGATGCAATGGAATTCCTCGTTAATAAACTGGCAATGACCAAGACCAATGACGATTTCTTCGAAatgat                                          >  1:5669186/1‑93 (MQ=255)

                                               aaTCATTCACCCGATGGGCGAAATCGATGCAATGGAATTCCTCGTTAATAAACTGGCAATGACCAAGACCAATGACGATTTCTTCGAAatgat                                          <  1:66461/93‑1 (MQ=255)

                                                aacATTCGCCAGATGGGCGTAGTCAATGCAATTGAATGCCTCGTTAATAAGCTGGCAATGACCAAGAACAATGTCGATTTCTTCGAAatgagg                                         <  1:2188704/91‑3 (MQ=255)

                                                aTCATTCACCCGATGGGCGAAATCGATGCAATGGAATTCCTCGTTAATAAACTGGCAATGACCAAGACCAATGACGATTTCTTCGAAatgatg                                         <  2:2673676/93‑1 (MQ=255)

                                                 tCATTCACCCGATGGGCGAAATCGATGCAATGGAATTCCTCGTTAATAAACTGGCAATGACCAAGACCAATGACGATTTCTTCGAAAtgatga                                        >  2:4360386/1‑93 (MQ=255)

                                                   aTTCACCCGATGGGCGAAATCGATGCAATGGAATTCCTCGTTAATAAACTGGCAATGACCAAGACCAATGACGATTTCTTCGAAATGATGaaa                                      >  1:5161847/1‑93 (MQ=255)

                                                    ttCACCCGATGGGCGAAATCGATGCAATGGAATTCCTCGTTAATAAACTGGCAATGACCAAGACCAATGACGATTTCTTCGAAATGATGAAAc                                     <  2:473457/93‑1 (MQ=255)

                                                      cACCCGATGGGCGAAATCGATGCAATGGAATTCCTCGTTAATAAACTGGCAATGACCAAGACCAATGACGATTTCTTCGAAATGATGAAACGc                                   >  2:5512605/1‑93 (MQ=255)

                                                       aCCCGATGGGCGAAATCGATGCAATGGAATTCCTCGTTAATAAACTGGCAATGACCAAGACCAATGACGATTTCTTCGAAATGATGAAACGCt                                  <  1:4725625/93‑1 (MQ=255)

                                                       aCCCGATGGGCGAAATCGATGCAATGGAATTCCTCGTTAATAAACTGGCAATGACCAAGACCAATGACGATTTCTTCGAAATGATGAAACGCt                                  >  2:5987681/1‑93 (MQ=255)

                                                        cccGATGGGCGAAATCGATGCAATGGAATTCCTCGTTAATAAACTGGCAATGACCAAGACCAATGACGATTTCTTCGAAATGATGAAACGCTc                                 <  2:2094041/93‑1 (MQ=255)

                                                        cccGATGGGCGAAATCGATGCAATGGAATTCCTCGTTAATAAACTGGCAATGACCAAGACCAATGACGATTTCTTCGAAATGATGAAACGCTc                                 >  1:2141102/1‑93 (MQ=255)

                                                         ccGATGGGCGAAATCGATGCAATGGAATTCCTCGTTAATAAACTGGCAATGACCAAGACCAATGACGATTTCTTCGAAATGATGAAACGCTCa                                >  2:545829/1‑93 (MQ=255)

                                                         ccGATGGGCGAAATCGATGCAATGGAATTCCTCGTTAATAAACTGGCAATGACCAAGACCAATGACGATTTCTTCGAAATGATGAAACGCTCa                                <  1:2639447/93‑1 (MQ=255)

                                                          cGATGGGCGAAATCGATGCTATGGAATTCCTCGTTAATAAACTGGCAATGACCAAGACCAATGACGATTTCTTCGAAATGATGAAACGCTCAt                               >  1:6565340/1‑93 (MQ=255)

                                                           gATGGGCGAAATCGATGCAATGGAATTCCTCGTTAATAAACTGGCAATGACCAAGACCAATGACGATTTCTTCGAAATGATGAAACGCTCATa                              <  2:290817/93‑1 (MQ=255)

                                                           gATGGGCGAAATCGATGCAATGGAATTCCTCGTTAATAAACTGGCAATGACCAAGACCAATGACGATTTCTTCGAAATGATGAAACGCTCATa                              >  2:1748792/1‑93 (MQ=255)

                                                            ttGGGCGAAATCGATGCAATGGAATTCCTCGTTAATAAACTGGCAATGACCAAGACCAATGACGATTTCTTCGAAATGATGAAACGCTCATaa                             >  1:1740896/2‑93 (MQ=255)

                                                            aTGGGCGAAATCGATGCAATGGAATTCCTCGTTAATAAACTGGCAATGACCAAGACCAATGACGATTTCTTCGAAATGATGAAACGCTCATaa                             >  2:281088/1‑93 (MQ=255)

                                                                gCGAAATCGATGCAATGGAATTCCTCGTTAATAAACTGGCAATGACCAAGACCAATGACGATTTCTTCGAAATGATGAAACGCTCATAAAttt                         <  1:738363/93‑1 (MQ=255)

                                                                gCGAAATCGATGCAATGGAATTCCTCGTTAATAAACTGGCAATGACCAAGACCAATGACGATTTCTTCGAAATGATGAAACGCTCATAAAttt                         >  2:3041804/1‑93 (MQ=255)

                                                                gCGAAATCGATGCAATGGAATTCCTCGTTAATAAACTGGCAATGACCAAGACCAATGACGATTTCTTCGAAATGATGAAACGCTCATAAAttt                         <  2:1111650/93‑1 (MQ=255)

                                                                 cGAAATCGATGCAATGGAATTCCTCGTTAATAAACTGGCAATGACCAAGACCAATGACGATTTCTTCGAAATGATGAAACGCTCATAAATTTg                        <  2:6080388/93‑1 (MQ=255)

                                                                 cGAAATCGATGCAATGGAATTCCTCGTTAATAAACTGGCAATGACCAAGACCAATGACGATTTCTTCGAAATGATGAAACGCTCATAAATTTg                        >  2:4945764/1‑93 (MQ=255)

                                                                  gAAATCGATGCAATGGAATTCCTCGTTAATAAACTGGCAATGACCAAGACCAATGACGATTTCTTCGAAATGATGAAACGCTCATAAATTTGt                       >  2:6499786/1‑93 (MQ=255)

                                                                   aaaTCGATGCAATGGAATTCCTCGTTAATAAACTGGCAATGACCAAGACCAATGACGATTTCTTCGAAATGATGAAACGCTCATAAATTTGTc                      >  2:5918447/1‑93 (MQ=255)

                                                                   aaaTCGATGCAATGGAATTCCTCGTTAATAAACTGGCAATGACCAAGACCAATGACGATTTCTTCGAAATGATGAAACGCTCATAAATTTGTc                      >  1:2963135/1‑93 (MQ=255)

                                                                    aaTCGATGCAATGGAATTCCTCGTTAATAAACTGGCAATGACCAAGACCAATGACGATTTCTTCGAAATGATGAAACGCTCATAAATTTGTCt                     >  1:3484656/1‑93 (MQ=255)

                                                                    aaTCGATGCAATGGAATTCCTCGTTAATAAACTGGCAATGACCAAGACCAATGACGATTTCTTCGAAATGATGAAACGCTCATAAATTTGTCt                     <  2:4035603/93‑1 (MQ=255)

                                                                    aaTCGATGCAATGGAATTCCTCGTTAATAAACTGGCAATGACCAAGACCAATGACGATTTCTTCGAAATGATGAAACGCTCATAAATTTGTCt                     <  2:3627271/93‑1 (MQ=255)

                                                                      tCGATGCAATGGAATTCCTCGTTAATAAACTGGCAATGACCAAGACCAATGACGATTTCTTCGAAATGATGAAACGCTCATAAATTTGTCTTa                   >  1:3332926/1‑93 (MQ=255)

                                                                      tCGATGCAATGGAATTCCTCGTTAATAAACTGGCAATGACCAAGACCAATGACGATTTCTTCGAAATGATGAAACGCTCATAAATTTGTCTTa                   >  2:2420/1‑93 (MQ=255)

                                                                       cGATGCAATGGAATTCCTCGTTAATAAACTGGCAATGACCAAGACCAATGACGATTTCTTCGAAATGATGAAACGCTCATAAATTTGTCTTAt                  <  1:828906/93‑1 (MQ=255)

                                                                           gCAATGGAATTCCTCGTTAATAAACTGGCAATGACCAAGACCAATGACGATTTCTTCGAAATGATGAAACGCTCATAAATTTGTCTTATGCCa              <  1:1845330/93‑1 (MQ=255)

                                                                             aaTGGAATTCCTCGTTAATAAACTGGCAATGACCAAGACCAATGACGATTTCTTCGAAATGATGAAACGCTCATAAATTTGTCTTATGCCaaa            >  1:2747701/1‑93 (MQ=255)

                                                                              aTGGAATTCCTCGTTAATAAACTGGCAATGACCAAGACCAATGACGATTTCTTCGAAATCATGAAACGCTCATAAATTTGTCTTATGCCaaaa           >  1:2036928/1‑93 (MQ=255)

                                                                               tGGAATTCCTCGTTAATAAACTGGCAATGACCAAGACCAATGACGATTTCTTCGAAATGATGAAACGCTCATAAATTTGTCTTATGCCaaaaa          >  2:4826094/1‑93 (MQ=255)

                                                                               tGGAATTCCTCGTTAATAAACTGGCAATGACCAAGACCAATGACGATTTCTTCGAAATGATGAAACGCTCATAAATTTGTCTTATGCCaaaaa          <  1:4406787/93‑1 (MQ=255)

                                                                                 gAATTCCTCGTTAATAAACTGGCAATGACCAAGACCAATGACGATTTCTTCGAAATGATGAAACGCTCATAAATTTGTCTTATGCCAAAAACg        >  1:4226819/1‑93 (MQ=255)

                                                                                    ttCCTCGTTAATAAACTGGCAATGACCAAGACCAATGACGATTTCTTCGAAATGATGAAACGCTCATAAATTTGTCTTATGCCAAAAACGCCa     <  2:1417094/93‑1 (MQ=255)

                                                                                      ccTCGTTAATAAACTGGCAATGACCAAGACCAATGACGATTTCTTCGAAATGATGAAACGCTCATAAATTTGTCTTATGCCAAAAACGCCACg   <  1:1624062/93‑1 (MQ=255)

                                                                                       cTCGTTAATAAACTGGCAATGACCAAGACCAATGACGATTTCTTCGAAATGATGAAACGCTCATAAATTTGTCTTATGCCAAAAACGCCACgt  >  1:2414419/1‑93 (MQ=255)

CTGCTCACGACTCAGGAAGAACTGCAGAAAATGTGGATCCTGCGCAAAATCATTCACCCGATGGGCGAAATCGATGCAATGGAATTCCTCATTAATAAACTGGCAATGACCAAGACCAATGACGATTTCTTCGAAATGATGAAACGCTCATAAATTTGTCTTATGCCAAAAACGCCACGT  >  NC_000913/3965139‑3965318

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 24 ≤` `ATCG/ATCG` `< 31 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`