BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,985,741	T→C	V313V (GTA→GTG)	hemX ←	uroporphyrinogen III methylase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,985,741	0	T	C	100.0%	1059.7 / NA	329	V313V (GTA→GTG)	hemX	uroporphyrinogen III methylase
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (158/171); total (158/171)

Only 100 of 343 total aligned reads displayed.

AAGATCCATCGAGATATTTTGCTGGCTTAACTGGTCCACCTCGTCGAGGAACGCTTTGGTGGTGGCATCATCAGTATCGTAGTAAGCACGTACCCAGGTGGAGACGTTCTCCAGCGCCTGGCGATAAGTCTCTTCCTGGTGACGCGGTACAGCTTGTGCTGCGACCAGCAGGCGAGAGCG  >  NC_000913/3985651‑3985830

 aGATCCATCGAGATATTTTGCTGGCTTAACTGGTCCACCTCGTCGAGGAACGCTTTGGTGGTGGCATCATCAGTATCGTAGTAAGCACGCAcc                                                                                        <  1:5247486/93‑1 (MQ=255)

 aGATCCATCGAGATATTTTGCTGGCTTAACTGGTCCACCTCGTCGAGGAACGCTTTGGTGGTGGCATCATCAGTATCGTAGTAAGCACGCAcc                                                                                        >  1:4545404/1‑93 (MQ=255)

 aGATCCATCGAGATATTTTGCTGGCTTAACTGGTCCACCTCGTCGAGGAACGCTTTGGTGGTGGCATCATCAGTATCGTAGTAAGCACGCAcc                                                                                        >  2:6552221/1‑93 (MQ=255)

  gATCCATCGAGATATTTTGCTGGCTTAACTGGTCCACCTCGTCGAGGAACGCTTTGGTGGTGGCATCATCAGTATCGTAGTAAGCACGCAccc                                                                                       <  2:6328936/93‑1 (MQ=255)

  gATCCATCGAGATATTTTGCTGGCTTAACTGGTCCACCTCGTCGAGGAACGCTTTGGTGGTGGCATCATCAGTATCGTAGTAAGCACGCAccc                                                                                       >  1:1570973/1‑93 (MQ=255)

  gATCCATCGAGATATTTTGCTGGCTTAACTGGTCCACCTCGTCGAGGAACGCTTTGGTGGTGGCATCATCAGTATCGTAGTAAGCACGCAccc                                                                                       >  1:4763419/1‑93 (MQ=255)

  gATCCATCGAGATATTTTGCTGGCTTAACTGGTCCACCTCGTCGAGGAACGCTTTGGTGGTGGCATCATCAGTATCGTAGTAAGCACGCAccc                                                                                       <  1:3694616/93‑1 (MQ=255)

   aTCCATCGAGATATTTTGCTGGCTTAACTGGTCCACCTCGTCGAGGAACGCTTTGGTGGTGGCATCATCAGTATCGTAGTAAGCACGCACCCa                                                                                      <  2:3850475/93‑1 (MQ=255)

   aTCCATCGAGATATTTTGCTGGCTTAACTGGTCCACCTCGTCGAGGAACGCTTTGGTGGTGGCATCATCAGTATCGTAGTAAGCACGCACCCa                                                                                      <  2:2717359/93‑1 (MQ=255)

   aTCCATCGAGATATTTTGCTGGCTTAACTGGTCCACCTCGTCGAGGAACGCTTTGGTGGTGGCATCATCAGTATCGTAGTAAGCACGCACCCa                                                                                      >  2:5511445/1‑93 (MQ=255)

    tCCATCGAGATATTTTGCTGGCTTAACTGGTCCACCTCGTCGAGGAACGCTTTGGTGGTGGCATCATCAGTATCGTAGTAAGCACGCACCCAg                                                                                     <  1:2026531/93‑1 (MQ=255)

    tCCATCGAGATATTTTGCTGGCTTAACTGGTCCACCTCGTCGAGGAACGCTTTGGTGGTGGCATCATCAGTATCGTAGTAAGCACGCACCCAg                                                                                     >  1:3832604/1‑93 (MQ=255)

     ccATCGAGATATTTTGCTGGCTTAACTGGTCCACCTCGTCGAGGAACGCTTTGGTGGTGGCATCATCAGTATCGTAGTAAGCACGCACCCAgg                                                                                    >  1:5827366/1‑93 (MQ=255)

     ccATCGAGATATTTTGCTGGCTTAACTGGTCCACCTCGTCGAGGAACGCTTTGGTGGTGGCATCATCAGTATCGTAGTAAGCACGCACCCAgg                                                                                    >  1:1893821/1‑93 (MQ=255)

      cATCGAGATATTTTGCTGGCTTAACTGGTCCACCTCGTCGAGGAACGCTTTGGTGGTGGCATCATCAGTATCGTGGTAAGCACGCACCCAGGt                                                                                   >  2:1038453/1‑93 (MQ=255)

      cATCGAGATATTTTGCTGGCTTAACTGGTCCACCTCGTCGAGGAACGCTTTGGTGGTGGCATCATCAGTATCGTAGTAAGCACGCACCCAGGt                                                                                   >  2:5403567/1‑93 (MQ=255)

      cATCGAGATATTTTGCTGGCTTAACTGGTCCACCTCGTCGAGGAACGCTTTGGTGGTGGCATCATCAGTATCGTAGTAAGCACGCACCCAGGt                                                                                   <  2:3284534/93‑1 (MQ=255)

       ctCGAGATATTTTGCTGGCTTAACTGGTCCACCTCGTCGAGGAACGCTTTGGTGGTGGCATCATCAGTATCGTAGTAAGCACGCACCCAGGTg                                                                                  <  1:3657086/92‑1 (MQ=255)

       aTCGAGATATTTTGCTGGCTTAACTGGTCCACCTCGTCGAGGAACGCTTTGGTGGTGGCATCATCAGTATCGTAGTAAGCACGCACCCAGGTg                                                                                  >  2:5811891/1‑93 (MQ=255)

        tCGAGATATTTTGCTGGCTTAACTGGTCCACCTCGTCGAGGAACGCTTTGGTGGTGGCATCATCAGTATCGTAGTAAGCACGCACCCAGGTgg                                                                                 <  2:2150674/93‑1 (MQ=255)

        tCGAGATATTTTGCTGGCTTAACTGGTCCACCTCGTCGAGGAACGCTTTGGTGGTGGCATCATCAGTATCGTAGTAAGCACGCACCCAGGTgg                                                                                 >  2:4091946/1‑93 (MQ=255)

        tCGAGATATTTTGCTGGCTTAACTGGTCCACCTCGTCGAGGAACGCTTTGGTGGTGGCATCATCAGTATCGTAGTAAGCACGCACCCAGGTgg                                                                                 >  1:2368784/1‑93 (MQ=255)

          gagaTATTTTGCTGGCTTAACTGGTCCACCTCGTCGAGGAACGCTTTGGTGGTGGCATCATCAGTATCGTAGTAAGCACGCACGCAGGTGgag                                                                               >  1:5120212/1‑93 (MQ=255)

          gagaTATTTTGCTGGCTTAACTGGTCCACCTCGTCGAGGAACGCTTTGGTGGTGGCATCATCAGTATCGTAGTAAGCACGCACCCAGGTGgag                                                                               >  1:4941124/1‑93 (MQ=255)

          gagaTATTTTGCTGGCTTAACTGGTCCACCTCGTCGAGGAACGCTTTGGTGGTGGCATCATCAGTATCGTAGTAAGCACGCACCCAGGTGgag                                                                               >  1:4247013/1‑93 (MQ=255)

           ggaTATTTTGCTGGCTTAACTGGTCCACCTCGTCGAGGAACGCTTTGGTGGTGGCATCATCAGTATCGTAGTAAGCACGCACCCAGGTGgaga                                                                              <  2:5342955/92‑1 (MQ=255)

           agaTATTTTGCTGGCTTAACTGGTCCACCTCGTCGAGGAACGCTTTGGTGGTGGCATCATCAGTATCGTAGTAAGCACGCACCCAGGTGgaga                                                                              >  1:2727502/1‑93 (MQ=255)

           agaTATTTTGCTGGCTTAACTGGTCCACCTCGTCGAGGAACGCTTTGGTGGTGGCATCATCAGTATCGTAGTAAGCACGCACCCAGGTGgaga                                                                              >  1:1685134/1‑93 (MQ=255)

           agaTATTTTGCTGGCTTAACTGGTCCACCTCGTCGAGGAACGCTTTGGTGGTGGCATCATCAGTATCGTAGTAAGCACGCACCCAGGTGgaga                                                                              <  1:5737858/93‑1 (MQ=255)

            gaTATTTTGCTGGCTTAACTGGTCCACCTCGTCGAGGAACGCTTTGGTGGTGGCATCATCAGTATCGTAGTAAGCACGCACCCAGGTGGAGAc                                                                             >  1:546619/1‑93 (MQ=255)

            gaTATTTTGCTGGCTTAACTGGTCCACCTCGTCGAGGAACGCTTTGGTGGTGGCATCATCAGTATCGTAGTAAGCACGCACCCAGGTGGAGAc                                                                             >  1:4154495/1‑93 (MQ=255)

             atatTTTGCTGGCTTAACTGGTCCACCTCGTCGAGGAACGCTTTGGTGGTGGCATCATCAGTATCGTAGTAAGCACGCACCCAGGTGGAGACg                                                                            >  1:1063701/1‑93 (MQ=255)

              tatTTTGCTGGCTTAACTGGTCCACCTCGTCGAGGAACGCTTTGGTGGTGGCATCATCAGTATCGTAGTAAGCACGCACCCAGGTGGAGACGt                                                                           >  1:5943757/1‑93 (MQ=255)

              tatTTTGCTGGCTTAACTGGTCCACCTCGTCGAGGAACGCTTTGGTGGTGGCATCATCAGTATCGTAGTAAGCACGCACCCAGGTGGAGACGt                                                                           <  1:6642999/93‑1 (MQ=255)

               atTTTGCTGGCTTAACTGGTCCACCTCGGCGAGGAACGCTTTGGTGGTGGCATCATCAGTATCGTAGTAAGCACGCACCCAGGTGGAGACGtt                                                                          <  2:2963453/93‑1 (MQ=255)

                ttttGCTGGCTTAACTGGTCCACCTCGTCGAGGAACGCTTTGGTGGTGGCATCATCAGTATCGTAGTAAGCACGCACCCAGGTGGAGACGTtc                                                                         <  1:5985388/93‑1 (MQ=255)

                   tGCTGGCTTAACTGGTCCACCTCGTCGAGGAACGCTTTGGTGGTGGCATCATCAGTATCGTAGTAAGCACGCACCCAGGTGGAGACGTTCTcc                                                                      <  2:2468618/93‑1 (MQ=255)

                    gCTGGCTTAACTGGTCCACCTCGTCGAGGAACGCTTTGGTGGTGGCATCATCAGTATCGTAGTAAGCACGCACCCAGGTGGAGACGTTCTCCa                                                                     >  2:131783/1‑93 (MQ=255)

                    gCTGGCTTAACTGGTCCACCTCGTCGAGGAACGCTTTGGTGGTGGCATCATCAGTATCGTAGTAAGCACGCACCCAGGTGGAGACGTTCTCCa                                                                     <  2:822004/93‑1 (MQ=255)

                      tGGCTTAACTGGTCCACCTCGTCGAGGAACGCTTTGGTGGTGGCATCATCAGTATCGTAGTAAGCACGCACCCAGGTGGAGACGTTCTCCAgc                                                                   >  2:2348954/1‑93 (MQ=255)

                      tGGCTTAACTGGTCCACCTCGTCGAGGAACGCTTTGGTGGTGGCATCATCAGTATCGTAGTAAGCACGCACCCAGGTGGAGACGTTCTCCAgc                                                                   >  2:2066771/1‑93 (MQ=255)

                         cTTAACTGGTCCACCTCGTCGAGGAACGCTTTGGTGGTGGCATCATCAGTATCGTAGTAAGCACGCACCCAGGTGGAGACGTTCTCCAGCGcc                                                                >  1:2611722/1‑93 (MQ=255)

                         cTTAACTGGTCCACCTCGTCGAGGAACGCTTTGGTGGTGGCATCATCAGTATCGTAGTAAGCACGCACCCAGGTGGAGACGTTCTCCAGCGcc                                                                <  2:5674804/93‑1 (MQ=255)

                          ttAACTGGTCCACCTCGTCGAGGAACGCTTTGGTGGTTGCATCATCAGGATCGTAGTAAGCACGCACCCAGGTGGAGACGTTCTCCAGCGCCt                                                               >  1:3379633/1‑93 (MQ=255)

                          ttAACTGGTCCACCTCGTCGAGGAACGCTTTGGTGGTGGCATCATCAGTATCGTAGTAAGCACGCACCCAGGTGGAGACGTTCTCCAGCGCCt                                                               <  1:5604577/93‑1 (MQ=255)

                          ttAACTGATCCACCTCGTCGAGGAACGCTTTGGTGGTGGCATCATCAGTATCGTAGTAAGCACGCACCCAGGTGGAGACGTTCTCCAGCGCCt                                                               <  2:86623/93‑1 (MQ=255)

                            aaCTGGTCCACCTCGTCGAGGAACGCTTTGGTGGTGGCATCATCAGTATCGTAGTAAGCACGCACCCAGGTGGAGACGTTCTCCAGCGCCTgg                                                             >  1:1153440/1‑93 (MQ=255)

                            aaCTGGTCCACCTCGTCGAGGAACGCTTTGGTGGTGGCATCATCAGTATCGTAGTAAGCACGCACCCAGGTGGAGACGTTCTCCAGCGCCTgg                                                             >  2:708274/1‑93 (MQ=255)

                              cTGGTCCACCTCGTCGAGGAACGCTTTGGTGGTGGCATCATCAGTATCGTAGTAAGCACGCACCCAGGTGGAGACGTTCTCCAGCGCCTGGCg                                                           >  1:1316904/1‑93 (MQ=255)

                                ggTCCACCTCGTCGAGGAACTCTTTGGTGGTGTCATCATCAGTATCGTAGTAAGCACGCACCCAGGTGGAGACGTTCTCCAGCGCCTGGCGAt                                                         <  2:6204659/93‑1 (MQ=255)

                                ggTCCACCTCGTCGAGGAACGCTTTGGGGGTGGCATCATCAGTATCGTAGTAAGCACGCACCCAGGTGGAGACGTTCTCCAGCGCCTGGCGAt                                                         >  1:294434/1‑93 (MQ=255)

                                 gTCCACCTCGTCGAGGAACGCTTTGGTGGTGGCATCATCAGTATCGTAGTAAGCACGCACCCAGGGGGAGCCGTTCTCCAGCGCCTGGCGATa                                                        >  1:1838713/1‑93 (MQ=255)

                                  tCCACCTCGTCGAGGAACGCTTTGGTGGTGGCATCATCAGTATCGTAGTAAGCACGCACCCAGGTGGAGACGTTCTCCAGCGCCTGGCGATaa                                                       >  2:4718586/1‑93 (MQ=255)

                                     aCCTCGTCGAGGAACGCTTTGGTGGTGGCATCATCAGTATCGTAGTAAGCACGCACCCAGGTGGAGACGTTCTCCAGCGCCTGGCGATAAGtc                                                    >  2:3540481/1‑93 (MQ=255)

                                     aCCTCGTCGAGGAACGCTTTGGTGGTGGCATCATCAGTATCGTAGTAAGCACGCACCCAGGTGGAGACGTTCTCCAGCGCCTGGCGATAAGtc                                                    >  2:3665811/1‑93 (MQ=255)

                                       cTCGTCGAGGAACGCTTTGGTGGTGGCATCATCAGTATCGTAGTAAGCACGCACCCAGGTGGAGACGTTCTCCAGCGCCTGGCGATAAGtctc                                                  <  1:3970938/93‑1 (MQ=255)

                                        tcgtctAGGAACGCTTTGGTGGTGGCATCATCAGTATCGTAGTAAGCACGCACCCAGGTGGAGACGTTCTCCAGCGCCTGGCGATAAGTctct                                                 <  2:4643233/93‑1 (MQ=255)

                                        tcgtcgAGGAACGCTTTGGTGGTGGCATCATCAGTATCGTAGTAAGCACGCACCCAGGTGGAGACGTTCTCCAGCGCCTGGCGATAAGTctct                                                 <  1:2274687/93‑1 (MQ=255)

                                        tcgtcgAGGAACGCTTTGGTGGTGGCATCATCAGTATCGTAGTAAGCACGCACCCAGGTGGAGACGTTCTCCAGCGCCTGGCGATAAGTctct                                                 <  2:5977130/93‑1 (MQ=255)

                                         cgtcgAGGAACGCTTTGGTGGTGGCATCATCAGTATCGTAGTAAGCACGCACCCAGGTGGAGACGTTCTCCAGCGCCTGGCGATAAGTCTCtt                                                >  2:4391421/1‑93 (MQ=255)

                                           tcgAGGAACGCTTTGGTGGTGGCATCATCAGTATCGTAGTAAGCACGCACCCAGGTGGAGACGTTCTCCAGCGCCTGGCGATAAGTCTCTTcc                                              <  1:6401175/93‑1 (MQ=255)

                                            cgAGGAACGCTTTGGTGGTGGCATCATCAGTATCGTAGTAAGCACGCACCCAGGTGGAGACGTTCTCCAGCGCCTGGCGATAAGTCTCTTCCt                                             >  1:244722/1‑93 (MQ=255)

                                            cgAGGAACGCTTTGGTGGTGGCATCATCAGTATCGTAGTAAGCACGCACCCAGGTGGAGACGTTCTCCAGCGCCTGGCGATAAGTCTCTTCCt                                             <  2:2872451/93‑1 (MQ=255)

                                              aGGAACGCTTTGGTGGTGGCATCATCAGTATCGTAGTAAGCACGCACCCAGGTGGAGACGTTCTCCAGCGCCTGGCGATAAGTCTCTTCCTgg                                           <  2:6121758/93‑1 (MQ=255)

                                               ggAACGCTTTGGTGGTGGCATCATCAGTATCGTAGTAAGCACGCACCCAGGTGGAGACGTTCTCCAGCGCCTGGCGATAAGTCTCTTCCTGGt                                          <  1:5388791/93‑1 (MQ=255)

                                               ggAACGCTTTGGTGGTGGCATCATCAGTATCGTAGTAAGCACGCACCCAGGTGGAGACGTTCTCCAGCGCCTGGCGATAAGTCTCTTCCTGGt                                          >  2:4806304/1‑93 (MQ=255)

                                                gAACGCTTTGGTGGTGGCATCATCAGTATCGTAGTAAGCACGCACCCAGGTGGAGACGTTCTCCAGCGCCTGGCGATAAGTCTCTTCCTGGTg                                         <  1:6324762/93‑1 (MQ=255)

                                                  aCGCTTTGGTGGTGGCATCATCAGTATCGTAGTAAGCACGCACCCAGGCGGAGACGTTCTCCAGCGCCTGGCGATAAGTCTCTTCCTGGTGAc                                       >  2:31409/1‑93 (MQ=255)

                                                     cTTTGGTGGTGGCATCATCAGTATCGTAGTAAGCACGCACCCAGGTGGAGACGTTCTCCAGCGCCTGGCGATAAGTCTCTTCCTGGTGAcgcg                                    <  2:149927/93‑1 (MQ=255)

                                                       ttGGTGGTGGCATCATCAGTATCGTAGTAAGCACGCACCCAGGTGGAGACGTTCTCCAGCGCCTGGCGATAAGTCTCTTCCTGGTGACGCGGt                                  >  1:3305925/1‑93 (MQ=255)

                                                        tggtggtggCATCATCAGTATCGTAGTAAGCACGCACCCAGGTGGAGACGTTCTCCAGCGCCTGGCGATAAGTCTCTTCCTGGTGACGCGGTa                                 <  2:3521575/93‑1 (MQ=255)

                                                         ggtggtggCATCATCAGTATCGTAGTAAGCACGCACCCAGGTGGAGACGTTCTCCAGCGCCTGGCGATAAGTCTCTTCCTGGTGACGCGGTAc                                >  2:5034449/1‑93 (MQ=255)

                                                         ggtggtggCATCATCAGTATCGTAGTAAGCACGCACCCAGGTGGAGACGTTCTCCAGCGCCTGGCGATAAGTCTCTTCCTGGTGACGCGGTAc                                <  1:4713879/93‑1 (MQ=255)

                                                          gtggtggCATCATCAGTATCGTAGTAAGCACGCACCCAGGTGGAGACGTTCTCCAGCGCCTGGCGATAAGTCTCTTCCTGGTGACGCGGTACa                               <  1:949199/93‑1 (MQ=255)

                                                            ggtggCATCATCAGTATCGTAGTAAGCACGCACCCAGGTGGAGACGTTCTCCAGCGCCTGGCGATAAGTCTCTTCCTGGTGACGCGGTACAGc                             <  2:1582081/93‑1 (MQ=255)

                                                             gtggCATCATCAGTATCGTAGTAAGCACGCACCCAGGTGGAGACGTTCTCCAGCGCCTGGCGATAAGTCTCTTCCTGGTGACGCGGTACAGCt                            <  1:728088/93‑1 (MQ=255)

                                                             gtggCATCATCAGTATCGTAGTAAGCACGCACCCAGGTGGAGACGTTCTCCAGCGCCTGGCGATAAGTCTCTTCCTGGTGACGCGGTACAGCt                            >  1:2530467/1‑93 (MQ=255)

                                                               ggCATCATCAGTATCGTAGTAAGCACGCACCCAGGTGGAGACGTTCTCCAGCGCCTGGCGATAAGTCTCTTCCTGGTGACGCGGTACAGCTtg                          >  2:431717/1‑93 (MQ=255)

                                                                gCATCATCAGTATCGTAGTAAGCACGCACCCAGGTGGAGACGTTCTCCAGCGCCTGGCGATAAGTCTCTTCCTGGTGACGCGGTACAGCTtgt                         <  2:5230268/93‑1 (MQ=255)

                                                                 catcatCAGTATCGTAGTAAGCACGCACCCAGGTGGAGACGTTCTCCAGCGCCTGGCGATAAGTCTCTTCCTGGTGACGCGGTACAGCTtgtg                        >  2:1173819/1‑93 (MQ=255)

                                                                  atcatcAGTATCGTAGTAAGCACGCACCCAGGTGGAGACGTTCTCCAGCGCCTGGCGATAAGTCTCTTCCTGGTGACGCGGTACAGCTTGtgc                       >  2:1855360/1‑93 (MQ=255)

                                                                     atcaGTATCGTAGTAAGCACGCACCCAGGTGGAGACGTTCTCCAGCGCCTGGCGATAAGTCTCTTCCTGGTGACGCGGTACAGCTTGtgctgc                    >  1:2680214/1‑93 (MQ=255)

                                                                      tcaGTATCGTAGTAAGCACGCACCCAGGTGGAGACGTTCTCCAGCGCCTGGCGATAAGTCTCTTCCTGGTGACGCGGTACAGCTTGTGCTGCg                   >  1:6227241/1‑93 (MQ=255)

                                                                        aGTATCGTAGTAAGCACGCACCCAGGTGGAGACGTTCTCCAGCGCCTGGCGATAAGTCTCTTCCTGGTGACGCGGTACAGCTTGTGCTGCGAc                 <  2:39198/93‑1 (MQ=255)

                                                                         gTATCGTAGTAAGCACGCACCCAGGTGGAGACGTTCTCCAGCGCCTGGCGATAAGTCTCTTCCTGGTGACGCGGTACAGCTTGTGCTGCGAcc                >  2:4115973/1‑93 (MQ=255)

                                                                              gtagtaAGCACGCACCCAGGTGGAGACGTTCTCCAGCGCCTGGCGATAAGTCTCTTCCTGGTGACGCGGTACAGCTTGTGCTGCGACcagcag           <  1:3588784/93‑1 (MQ=255)

                                                                               tagtaAGCACGCACCCAGGTGGAGACGTTCTCCAGCGCCTGGCGATAAGTCTCTTCCTGGTGACGCGGTACAGCTTGTGCTGCGACCAGCAgg          >  1:5171495/1‑93 (MQ=255)

                                                                               tagtaAGCACGCACCCAGGTGGAGACGTTCTCCAGCGCCTGGCGATAAGTCTCTTCCTGGTGACGCGGTACAGCTTGTGCTGCGACCAGCAgg          >  2:3588686/1‑93 (MQ=255)

                                                                                agtaAGCACGCACCCAGGTGGAGACGTTCTCCAGCGCCTGGCGATAAGTCTCTTCCTGGTGACGCGGTACAGCTTGTGCTGCGACCAGCAGGc         <  1:436565/93‑1 (MQ=255)

                                                                                 gtaAGCACGCACCCAGGTGGAGACGTTCTCCAGCGCCTGGCGATAAGTCTCTTCCTGGTGACGCGGTACAGCTTGTGCTGCGACCAGCAGGCg        <  2:3398124/93‑1 (MQ=255)

                                                                                  taAGCACGCCCCCAGGTGGAGGCGTTCTCCAGCGCCTGGCGATAAGTCTCTTCCTGGTGACGCGGTACAGCTTGTGCTGCGACCAGCAGGCga       <  1:4065791/93‑1 (MQ=255)

                                                                                  taAGCACGCACCCAGGTGGAGACGTTCTCCAGCGCCTGGCGATAAGTCTCTTCCTGGTGACGCGGTACAGCTTGTGCTGCGACCAGCAGGCga       >  2:4602235/1‑93 (MQ=255)

                                                                                  taAGCACGCACCCAGGTGGAGACGTTCTCCAGCGCCTGGCGATAAGTCTCTTCCTGGTGACGCGGTACAGCTTGTGCTGCGACCAGCAGGCga       <  1:821767/93‑1 (MQ=255)

                                                                                   aaGCACGCACCCAGGTGGAGACGTTCTCCAGCGCCTGGCGATAAGTCTCTTCCTGGTGACGCGGTACAGCTTGTGCTGCGACCAGCAGGCgag      <  1:5544331/93‑1 (MQ=255)

                                                                                   aaGCACGCACCCAGGTGGAGACGTTCTCCAGCGCCTGGCGATAAGTCTCTTCCTGGTGACGCGGTACAGCTTGTGCTGCGACCAGCAGGCgag      <  1:6124496/93‑1 (MQ=255)

                                                                                   aaGCACGCACCCAGGTGGAGACGTTCTCCAGCGCCTGGCGATAAGTCTCTTCCTGGTGACGCGGTACAGCTTGTGCTGCGACCAGCAGGCgag      >  1:1013326/1‑93 (MQ=255)

                                                                                    aGCACGCACCCAGGTGGAGACGTTCTCCAGCGCCTGGCGATAAGTCTCTTCCTGGTGACGCGTTACAGCTTGTGCTGCTACCAGCAGGCgaga     <  1:3895861/93‑1 (MQ=255)

                                                                                    aGCACGCACCCAGGTGGAGACGTTCTCCAGCGCCTGGCGATAAGTCTCTTCCTGGTGACGCGGTACAGCTTGTGCTGCGACCAGCAGGCgaga     >  2:5101321/1‑93 (MQ=255)

                                                                                    aGCACGCACCCAGGTGGAGACGTTCTCCAGCGCCTGGCGATAAGTCTCTTCCTGGTGACGCGGTACAGCTTGTGCTGCGACCAGCAGGCgaga     <  2:2615356/93‑1 (MQ=255)

                                                                                     gcacgcacCCAGGTGGAGACGTTCTCCAGCGCCTGGCGATAAGTCTCTTCCTGGTGACGCGGTACAGCTTGTGCTGCGACCAGCAGGCGagag    >  1:1400139/1‑93 (MQ=255)

AAGATCCATCGAGATATTTTGCTGGCTTAACTGGTCCACCTCGTCGAGGAACGCTTTGGTGGTGGCATCATCAGTATCGTAGTAAGCACGTACCCAGGTGGAGACGTTCTCCAGCGCCTGGCGATAAGTCTCTTCCTGGTGACGCGGTACAGCTTGTGCTGCGACCAGCAGGCGAGAGCG  >  NC_000913/3985651‑3985830

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 20 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`