BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	539,651	A→G	L427L (TTA→TTG)	ybbX →	putative hydrolase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	539,651	0	A	G	99.7%	1033.5 / ‑4.9	315	L427L (TTA→TTG)	ybbX	putative hydrolase
Reads supporting (aligned to +/- strand): ref base A (1/0); new base G (151/163); total (152/163)
Fisher's exact test for biased strand distribution p-value = 4.83e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.52e-01

Only 100 of 320 total aligned reads displayed.

TGTTCTTACCAATGACGATCTGGAATATCGCCACAAAGTCAGCCCGTATGTTGGCCGTACCATTGGCGCGCGTATCACGAAAACCATCTTACGTGGTGATGTGATTTACGACATTGAACAGGGCTTCCCTGTTGCGCCGAAAGGTCAATTTATCCTTAAACATCAGCAGTAATCTGGCCC  >  NC_000913/539561‑539740

    cTTACCAATGACGATCTGGAATATCGCCACAAAGTCAGCCCGTATGTTGGCCGTACCATTGGCGCGCGTATCACGAAAACCATCTTGCgtggt                                                                                     <  1:547436/93‑1 (MQ=255)

    cTTACCAATGACGATCTGGAATATCGCCACAAAGTCAGCCCGTATGTTGGCCGTACCATTGGCGCGCGTATCACGAAAACCATCTTGCgtggg                                                                                     >  2:3726036/1‑92 (MQ=255)

      tACCAATGACGATCTGGAATATCGCCACAAAGTCAGCCCGTATGTTGGCCGTACCATTGGCGCGCGTATCACGAAAACCATCTTGCGTGgtga                                                                                   <  1:5996209/93‑1 (MQ=255)

      tACCAATGACGATCTGGAATATCGCCACAAAGTCAGCCCGTATGTTGGCCGTACCATTGGCGCGCGTATCACGAAAACCATCTTGCGTGgtga                                                                                   >  2:5041178/1‑93 (MQ=255)

       aCCAATGACGATCTGGAATATCGCCACAAAGTCAGCCCGTATGTTGGCCGTACCATTGGCGCGCGTATCACGAAAACCATCTTGCGTGgtgat                                                                                  <  1:1160588/93‑1 (MQ=255)

       aCCAATGACGATCTGGAATATCGCCACAAAGTCAGCCCGTATGTTGGCCGTACCATTGGCGCGCGTATCACGAAAACCATCTTGCGTGgtgat                                                                                  <  2:5850390/93‑1 (MQ=255)

        ccAATGACGATCTGGAATATCGCCACAAAGTCAGCCCGTATGTTGGCCGTACCATTGGCGCGCGTATCACGAAAACCATCTTGCGTGgtgatg                                                                                 >  2:1407982/1‑93 (MQ=255)

         cAATGACGATCTGGAATATCGCCACAAAGTCAGCCCGTATGTTGGCCGTACCATTGGCGCGCGTATCACGAAAACCATCTTGCGTGgtgatgt                                                                                >  1:1875430/1‑93 (MQ=255)

          aaTGACGATCTGGAATATCGCCACAAAGTCAGCCCGTATGTTGGCCGTACCATTGGCGCGCGTATCACGAAAACCATCTTGCGTGgtgatgtg                                                                               >  1:2365798/1‑93 (MQ=255)

           aTGACGATCTGGAATATCGCCACAAAGTCAGCCCGTATGTTGGCCGTACCATTGGCGCGCGTATCACGAAAACCATCTTGCGTGgtgatgtga                                                                              >  2:4803316/1‑93 (MQ=255)

           aTGACGATCTGGAATATCGCCACAAAGTCAGCCCGTATGTTGGCCGTACCATTGGCGCGCGTATCACGAAAACCATCTTGCGTGgtgatgtga                                                                              >  2:1796058/1‑93 (MQ=255)

            tGACGATCTGGTATATCGCCACAAAGTCAGCCCGTATGTTGGCCGTACCATTGGCGCGCGTATCACGAAAACCATCTTGCGTGgtgatgtgat                                                                             >  1:4585923/1‑93 (MQ=255)

            tGACGATCTGGAATATCGCCACAAAGTCAGCCCGTATGTTGGCCGTACCATTGGCGCGCGTATCACGAAAACCATCTTGCGTGgtgatgtgat                                                                             <  2:1119386/93‑1 (MQ=255)

             gACGATCTGGAATATCGCCACAAAGTCAGCCCGTATGTTGGCCGTACCATTGGCGCGCGTATCACGAAAACCATCTTGCGTGGTGATGTGAtt                                                                            >  1:1649542/1‑93 (MQ=255)

             gACGATCTGGAATATCGCCACAAAGTCAGCCCGTATGTTGGCCGTACCATTGGCGCGCGTATCACGAAAACCATCTTGCGTGGTGATGTGAtt                                                                            >  2:6704397/1‑93 (MQ=255)

              aCGATCTGGAATATCGCCACAAAGTCAGCCCGTATTTTGGCCGTACCATTGGCGCGCGTATCACGAAAACCATCTTGCGTGGTGATGTGAttt                                                                           <  1:1566232/93‑1 (MQ=255)

                gATCTGGAATATCGCCACAAAGTCAGCCCGTATGTTGGCCGTACCATTGGCGCGCGTATCACGAAAACCATCTTGCGTGGTGATGTGATTTAc                                                                         >  1:3633564/1‑93 (MQ=255)

                gATCTGGAATATCGCCACAAAGTCAGCCCGTATGTTGGCCGTACCATTGGCGCGCGTATCACGAAAACCATCTTGCGTGGTGATGTGATTTAc                                                                         <  2:2893586/93‑1 (MQ=255)

                  tCTGGAATATCGCCACAAAGTCAGCCCGTATGTTGGCCGTACCATTGGCGCGCGTATCACGAACACCATCTTGCGTGGTGATGTGATTTACGa                                                                       >  2:2067326/1‑93 (MQ=255)

                  tCTGGAATATCGCCACAAAGTCAGCCCGTATGTTGGCCGTACCATTGGCGCGCGTATCACGAAAACCATCTTGCGTGGTGATGTGATTTACGa                                                                       >  1:4451876/1‑93 (MQ=255)

                  tCTGGAATATCGCCACAAAGTCAGCCCGTATGTTGGCCGTACCATTGGCGCGCGTATCACGAAAACCATCTTGCGTGGTGATGTGATTTACGa                                                                       >  1:5728657/1‑93 (MQ=255)

                       aaTATCGCCACAAAGTCAGCCCGTATGTTGGCCGTACCATTGGCGCGCGTATCACGAAAACCATCTTGCGTGGTGATGTGATTTACGACATTg                                                                  >  2:3431635/1‑93 (MQ=255)

                        atatCGCCACAAAGTCAGCCCGTATGTTGGCCGTACCATTGGCGCGCGTATCACGAAAACCATCTTGCGTGGTGATGTGATTTACGACATTGa                                                                 >  1:3780050/1‑93 (MQ=255)

                         tatCGCCACAAAGTCAGCCCGTATGTTGGCCGTACCATTGGCGCGCGTATCACGAAAACCATCTTGCGTGGTGATGTGATTTACGACATTGaa                                                                >  2:4345702/1‑93 (MQ=255)

                          atCGCCACAAAGTCAGCCCGTATGTTGGCCGTACCATTGGCGCGCGTATCACGAAAACCATCTTGCGTGGTGATGTGATTTACGACATTGAAc                                                               <  1:1372086/93‑1 (MQ=255)

                           tCGCCACAAAGTCAGCCCGTATGTTGGCCGTACCATTGGCGCGCGTATCACGAAAACCATCTTGCGTGGTGATGTGATTTACGACATTGAACa                                                              <  2:1320213/93‑1 (MQ=255)

                              ccACAAAGTCAGCCCGTATGTTGGCCGTACCATTGGCGCGCGTATCACGAAAACCATCTTGCGTGGTGATGTGATTTACGACATTGAACAggg                                                           >  2:804049/1‑93 (MQ=255)

                               cacaAAGTCAGCCCGTATGTTGGCCGTACCATTGGCGCGCGTATCACGAAAACCATCTTGCGTGTTGATGTGATTTACGACATTGAACAGGGc                                                          <  1:6637012/93‑1 (MQ=255)

                               cacaAAGTCAGCCCGTATGTTGGCCGTACCATTGGCGCGCGTATCACGAAAACCATCTTGCGTGGTGATGTGATTTACGACATTGAACAGGGc                                                          <  1:389238/93‑1 (MQ=255)

                               cacaAAGTCAGCCCGTATGTTGGCCGTACCATTGGCGCGCGTATCACGAAAACCATCTTGCGTGGTGATGTGATTTACGACATTGAACAGGGc                                                          <  2:2481986/93‑1 (MQ=255)

                                   aaGTCAGCCCGTATGTTGGCCGTACCATTGGCGCGCGTATCACGAAAACCATCTTGCGTGGTGATGTGATTTACGACATTGAACAGGGCTTcc                                                      <  2:5625665/93‑1 (MQ=255)

                                   aaGTCAGCCCGTATGTTGGCCGTACCATTGGCGCGCGTATCACGAAAACCATCTTGCGTGGTGATGTGATTTACGACATTGAACAGGGCTTcc                                                      <  2:3526380/93‑1 (MQ=255)

                                    aGTCAGCCCGTATGTTGGCCGTACCATTGGCGCGCGTATCACGAAAACCATCTTGCGTGGTGATGTGATTTACGACATTGAACAGGGCTTccc                                                     <  2:5575682/93‑1 (MQ=255)

                                    aGTCAGCCCGTATGTTGGCCGTACCATTGGCGCGCGTATCACGAAAACCATCTTGCGTGGTGATGTGATTTACGACATTGAACAGGGCTTccc                                                     <  2:391225/93‑1 (MQ=255)

                                     gTCAGCCCGTATGTTGGCCGTACCATTGGCGCGCGTATCACGAAAACCATCTTGCGTGGTGATGTGATTTACGACATTGAACAGGGCTTCCCt                                                    >  1:1714559/1‑93 (MQ=255)

                                      tCAGCCCGTATGTTGGCCGTACCATTGGCGCGCGTATCACGAAAACCATCTTGCGTGGTGATGTGATTTACGACATTGAACAGGGCTTCCCTg                                                   <  2:2878782/93‑1 (MQ=255)

                                      tCAGCCCGTATGTTGGCCGTACCATTGGCGCGCGTATCACGAAAACCATCTTGCGTGGTGATGTGATTTACGACATTGAACAGGGCTTCCCTg                                                   >  2:5492087/1‑93 (MQ=255)

                                        aGCCCGTATGTTGGCCGTACCATTGGCGCGCGTATCACGAAAACCATCTTGCGTGGTGATGTGATTTACGACATTGAACAGGGCTTCCCTGtt                                                 <  1:5859245/93‑1 (MQ=255)

                                        aGCCCGTATGTTGGCCGTACCATTGGCGCGCGTATCACGAAAACCATCTTGCGTGGTGATGTGATTTACGACATTGAACAGGGCTTCCCTGtt                                                 >  1:4980488/1‑93 (MQ=255)

                                          cccGTATGTTGGCCGTACCATTGGCGCGCGTATCACGAAAACCATCTTGCGTGGTGATGTGATTTACGACATTGAACAGGGCTTCCCTGTTgc                                               <  2:4018699/93‑1 (MQ=255)

                                          cccGTATGTTGGCCGTACCATTGGCGCGCGTATCACGAAAACCATCTTGCGTGGTGATGTGATTTACGACATTGAACAGGGCTTCCCTGTTgc                                               <  1:2037875/93‑1 (MQ=255)

                                            cGTATGTTGGCCGTACCATTGGCGCGCGTATCACGAAAACCATCTTGCGTGGTGATGTGATTTACGACATTGAACAGGGCTTCCCTGTTgcgc                                             >  2:2108925/1‑93 (MQ=255)

                                            cGTATGTTGGCCGTACCATTGGCGCGCGTATCACGAAAACCATCTTGCGTGGTGATGTGATTTACGACATTGAACAGGGCTTCCCTGTTgcgc                                             >  1:4387419/1‑93 (MQ=255)

                                            cGTATGTTGGCCGTACCATTGGCGCGCGTATCACGAAAACCATCTTGCGTGGTGATGTGATTTACGACATTGAACAGGGCTTCCCTGTTgcgc                                             >  1:913387/1‑93 (MQ=255)

                                             gTATGTTGGCCGTACCATTGGCGCGCGTATCACGAAAACCATCTTGCGTGGTGATGTGATTTATGACATTGAACAGGGCTTCCCTGTTGCGcc                                            >  1:3595645/1‑93 (MQ=255)

                                             gTATGTTGGCCGTACCATTGGCGCGCGTATCACGAAAACCATCTTGCGTGGTGATGTGATTTACGACATTGAACAGGGCTTCCCTGTTGCGcc                                            >  1:176367/1‑93 (MQ=255)

                                               aTGTTGGCCGTACCATTGGCGCGCGTATCACGAAAACCATCTTGCGTGGTGATGTGATTTACGACATTGAACAGGGCTTCCCTGTTGCGCCGa                                          <  1:1413962/93‑1 (MQ=255)

                                                tGTTGGCCGTACCATTGGCGCGCGTATCACGAAAACCATCTTGCGTGGTGATGTGATTTACGACATTGAACAGGGCTTCCCTGTTGCGCCGaa                                         >  2:6387975/1‑93 (MQ=255)

                                                  ttGGCCGTACCATTGGCGCGCGTATCACGAAAACCATCTTGCGTGGTGATGTGATTTACGACATTGAACAGGGCTTCCCTGTTGCGCCGAAAg                                       >  2:3298203/1‑93 (MQ=255)

                                                  ttGGCCGTACCATTGGCGCGCGTATCACGAAAACCATCTTGCGTGGTGATGTGATTTACGACATTGAACAGGGCTTCCCTGTTGCGCCGAAAg                                       <  2:5282338/93‑1 (MQ=255)

                                                    ggCCGTACCATTGGCGCGCGTATCACGAAAACCATCTTGCGTGGTGATGTGATTTACGACATTGAACAGGGCTTCCCTGTTGCGCCGAAAGGt                                     <  1:2751062/93‑1 (MQ=255)

                                                    ggCCGTACCATTGGCGCGCGTATCACGAAAACCATCTTGCGTGGTGATGTGATTTACGACATTGAACAGGGCTTCCCTGTTGCGCCGAAAGGt                                     <  1:2097897/93‑1 (MQ=255)

                                                     gCCGTACCATTGGCGCGCGTATCACGAAAACCATCTTGCGTGGTGATGTGATTTACGACATTGAACAGGGCTTCCCTGTTGCGCCGAAAGGTc                                    <  1:2467129/93‑1 (MQ=255)

                                                     gCCGTACCATTGGCGCGCGTATCACGAAAACCATCTTGCGTGGTGATGTGATTTACGACATTGAACAGGGCTTCCCTGTTGCGCCGAAAGGTc                                    <  2:2657437/93‑1 (MQ=255)

                                                       cGTACCATTGGCGCGCGTATCACGAAAACCATCTTGCGTGGTGATGTGATTTACGACATTGAACAGGGCTTCCCTGTTGCGCCGAAAGGTCaa                                  <  1:64031/93‑1 (MQ=255)

                                                       cGTACCATTGGCGCGCGTATCACGAAAACCATCTTGCGTGGTGATGTGATTTACGACATTGAACAGGGCTTCCCTGTTGCGCCGAAAGGTCaa                                  <  1:4396134/93‑1 (MQ=255)

                                                        gTACCATTGGCGCGCGTATCACGAAAACCATCTTGCGTGGTGATGTGATTTACGACATTGAACAGGGCTTCCCTGTTGCGCCGAAAGGTCAAt                                 >  2:6281977/1‑93 (MQ=255)

                                                        gTACCATTGGCGCGCGTATCACGAAAACCATCTTGCGTGGTGATGTGATTTACGACATTGAACAGGGCTTCCCTGTTGCGCCGAAAGGTCAAt                                 >  1:3494817/1‑93 (MQ=255)

                                                        gTACCATTGGCGCGCGTATCACGAAAACCATCTTGCGTGGTGATGTGATTTACGACATTGAACAGGGCTTCCCTGTTGCGCCGAAAGGTCAAt                                 <  1:2601287/93‑1 (MQ=255)

                                                           ccATTGGCGCGCGTATCACGAAAACCATCTTGCGTGGTGATGTGATTTACGACATTGAACAGGGCTTCCCTGTTGCGCCGAAAGGTCAATTTa                              <  2:732680/93‑1 (MQ=255)

                                                           ccATTGGCGCGCGTATCACGAAAACCATCTTGCGTGGTGATGTGATTTACGACATTGAACAGGGCTTCCCTGTTGCGCCGAAAGGTCAATTTa                              >  1:4891408/1‑93 (MQ=255)

                                                            cATTGGCGCGCGTATCACGAAAACCATCTTGCGTGGTGATGTGATTTACGACATTGAACAGGGCTTCCCTGTTGCGCCGAAAGGTCAATTTAt                             >  1:5152772/1‑93 (MQ=255)

                                                              ttGGCGCGCGTATCACGAAAACCATCTTGCGTGGTGATGTGATTTACGACATTGAACAGGGCTTCCCTGTTGCGCCGAAAGGTCAATTTATcc                           >  2:2428191/1‑93 (MQ=255)

                                                              ttGGCGCGCGTATCACGAAAACCATCTTGCGTGGTGATGTGATTTACGACATTGAACAGGGCTTCCCTGTTGCGCCGAAAGGTCAATTTATcc                           <  2:4926141/93‑1 (MQ=255)

                                                              ttGGCGCGCGTATCACGAAAACCATCTTGCGTGGTGATGTGATTTACGACATTGAACAGGGCTTCCCTGTTGCGCCGAAAGGTCAATTTATcc                           >  1:4795575/1‑93 (MQ=255)

                                                              ttGGCGCGCGTATCACGAAAACCATCTTGCGTGGTGATGTGATTTACGACATTGAACAGGGCTTCCCTGTTGCGCCGAAAGGTCAATTTATcc                           <  1:3245484/93‑1 (MQ=255)

                                                               tGGCGCGCGTATCACGAAAACCATCTTGCGTGGTGATGTGATTTACGACATTGAACAGGGCTTCCCTGTTGCGCCGAAAGGTCAATTTATCCt                          <  1:4205732/93‑1 (MQ=255)

                                                                ggCGCGCGTATCACGAAAACCATCTTGCGTGGTGATGTGATTTACGACATTGAACAGGGCTTCCCTGTTGCGCCGAAAGGTCAATTTATCCtt                         <  2:601880/93‑1 (MQ=255)

                                                                ggCGCGCGTATCACGAAAACCATCTTGCGTGGTGATGTGATTTACGACATTGAACAGGGCTTCCCTGTTGCGCCGAAAGGTCAATTTATCCtt                         <  1:80960/93‑1 (MQ=255)

                                                                 gcgcgcGTATCACGAAAACCATCTTGCGTGGTGATGTGATTTACGACATTGAACAGGGCTTCCCTGTTGCGCCGAAAGGTCAATTTATCCTTa                        <  1:2641560/93‑1 (MQ=255)

                                                                   gcgcgTATCACGAAAACCATCTTGCGTGGTGATGTGATTTACGACATTGAACAGGGCTTCCCTGTTGCGCCGAAAGGTCAATTTATCCTTaaa                      <  2:2355469/93‑1 (MQ=255)

                                                                    cgcgTATCACGAAAACCATCTTGCGTGGTGATGTGATTTACGACATTGAACAGGGCTTCCCTGTTGCGCCGAAAGGTCAATTTATCCTTAAAc                     <  1:5163924/93‑1 (MQ=255)

                                                                    cgcgTATCACGAAAACCATCTTGCGTGGTGATGTGATTTACGACATTGAACAGGGCTTCCCTGTTGCGCCGAAAGGTCAATTTATCCTTAAAc                     <  2:6168945/93‑1 (MQ=255)

                                                                    cgcgTATCACGAAAACCATCTTGCGTGGTGATGTGATTTACGACATTGAACAGGGCTTCCCTGTTGCGCCGAAAGGTCAATTTATCCTTAAAc                     >  1:299208/1‑93 (MQ=255)

                                                                      cgTATCACGAAAACCATCTTGCGTGGTGATGTGATTTACGACATTGAACAGGGCTTCCCTGTTGCGCCGAAAGGTCAATTTATCCTTAAACAt                   >  1:974073/1‑93 (MQ=255)

                                                                       gTATCACGAAAACCATCTTGCGTGGTGATGTGATTTACGACATTGAACAGGGCTTCCCTGTTGCGCCGAAAGGTCAATTTATCCTTAAACATc                  >  1:1318081/1‑93 (MQ=255)

                                                                       gTATCACGAAAACCATCTTGCGTGGTGATGTGATTTACGACATTGAACAGGGCTTCCCTGTTGCGCCGAAAGGTCAATTTATCCTTAAACATc                  <  1:1074805/93‑1 (MQ=255)

                                                                       gTATCACGAAAACCATCTTGCGTGGTGATGTGATTTACGACATTGAACAGGGCTTCCCTGTTGCGCCGAAAGGTCAATTTATCCTTAAACATc                  <  2:6329511/93‑1 (MQ=255)

                                                                        tATCACGAAAACCATCTTGCGTGGTGATGTGATTTACGACATTGAACAGGGCTTCCCTGTTGCGCCGAAAGGTCAATTTATCCTTAAACATca                 <  2:4621438/93‑1 (MQ=255)

                                                                         aTCACGAAAACCATCTTGCGTGGTGATGTGATTTACGACATTGAACAGGGCTTCCCTGTTGCGCCGAAAGGTCAATTTATCCTTAAACATcag                >  2:166818/1‑93 (MQ=255)

                                                                         aTCACGAAAACCATCTTGCGTGGTGATGTGATTTACGACATTGAACAGGGCTTCCCTGTTGCGCCGAAAGGTCAATTTATCCTTAAACATcag                <  2:5002435/93‑1 (MQ=255)

                                                                           cACGAAAACCATCTTGCGTGGTGATGTGATTTACGACATTGAACAGGGCTTCCCTGTTGCGCCGAAAGGTCAATTTATCCTTAAACATcagca              >  2:4068637/1‑93 (MQ=255)

                                                                             cGAAATCCATCTTGCGTGGTGATGTGATTTACGACATTGAACAGGGCTTCCCTGTTGCGCCGAAAGGTCAATTTATCCTTAAACATCAGCAGt            <  2:5904662/93‑1 (MQ=255)

                                                                             cGAAAACCATCTTGCGTGGTGATGTGATTTACGACATTGAACAGGGCTTCCCTGTTGCGCCGAAAGGTCAATTTATCCTTAAACATCAGCAGt            <  2:4385900/93‑1 (MQ=255)

                                                                             cGAAAACCATCTTGCGTGGTGATGTGATTTACGACATTGAACAGGGCTTCCCTGTTGCGCCGAAAGGTCAATTTATCCTTAAACATCAGCAGt            <  1:6177651/93‑1 (MQ=255)

                                                                              gAAAACCATCTTGCGTGGTGATGTGATTTACGACATTGAACAGGGCTTCCCTGTTGCGCCGAAAGGTCAATTTATCCTTAAACATCAGCAGTa           <  2:2582431/93‑1 (MQ=255)

                                                                               aaaaCCATCTTGCGTGGTGATGTGATTTACGACATTGAACAGGGCTTCCCTGTTGCGCCGAAAGGTCAATTTATCCTTAAACATCAGCAGTaa          <  2:3845506/93‑1 (MQ=255)

                                                                               aaaaCCATCTTGCGTGGTGATGTGATTTACGACATTGAACAGGGCTTCCCTGTTGCGCCGAAAGGTCAATTTATCCTTAAACATCAGCAGTaa          <  1:568859/93‑1 (MQ=255)

                                                                                aaaCCATCTTGCGTGGTGATGTGATTTACGACATTGAACAGGGCTTCCCTGTTGCGCCGAAAGGTCAATTTATCCTTAAACATCAGCAGTAAt         <  2:1572172/93‑1 (MQ=255)

                                                                                aaaCCATCTTGCGTGGTGATGTGATTTACGACATTGAACAGGGCTTCCCTGTTGCGCCGAAAGGTCAATTTATCCTTAAACATCAGCAGTAAt         >  2:3192099/1‑93 (MQ=255)

                                                                                aaaCCATCTTGCGTGGTGATGTGATTTACGACATTGAACAGGGCTTCCCTGTGGCGCCGAAAGGTCAATTTATCCTTAAACATCAGCAGTAAt         <  1:5432042/93‑1 (MQ=255)

                                                                                 aaCCATCTTGCGTGGTGATGTGATTTACGACATTGAACAGGGCTTCCCTGTTGCGCCGAAAGGTCAATTTATCCTTAAACATCAGCAGTAATc        <  2:4525270/93‑1 (MQ=255)

                                                                                  aCCATCTTGCGTGGTGATGTGATTTACGACATTGAACAGGGCTTCCCTGTTGCGCCGAAAGGTCAATTTATCCTTAAACATCAGCAGTAATCt       <  2:2832434/93‑1 (MQ=255)

                                                                                   ccATCTTGCGTGGTGATGTGATTTACGACATTGAACAGGGCTTCCCTGTTGCGCCGAAAGGTCAATTTATCCTTAAACATCAGCAGTAATCTg      >  2:1022757/1‑93 (MQ=255)

                                                                                    cATCTTGCGTGGTGATGTGATTTACGACATTGAACAGGGCTTCCCTGTTGCGCCGAAAGGTCAATTTATCCTTAAACATCAGCAGTAATCTgg     >  2:5423527/1‑93 (MQ=255)

                                                                                    cATCTTGCGTGGTGATGTGATTTACGACATTGAACAGGGCTTCCCTGTTGCGCCGAAAGGTCAATTTATCCTTAAACATCAGCAGTAATCTgg     >  2:3073915/1‑93 (MQ=255)

                                                                                     aTCTTGCGTGGTGATGTGATTTACGACATTGAACAGGGCTTCCCTGTTGCGCCGAAAGGTCAATTTATCCTTAAACATCAGCAGTAATCTGGc    <  2:5946142/93‑1 (MQ=255)

                                                                                     aTCTTGCGTGGTGATGTGATTTACGACATTGAACAGGGCTTCCCTGTTGCGCCGAAAGGTCAATTTATCCTTAAACATCAGCAGTAATCTGGc    >  1:2128870/1‑93 (MQ=255)

                                                                                      tCTTGCGTGGTGATGTGATTTACGACATTGAACAGGGCTTCCCTGTTGCGCCGAAAGGTCAATTTATCCTTAAACATCAGCAGTAATCTGGcc   >  2:5129519/1‑93 (MQ=255)

                                                                                       cTTGCGTGGTGATGTGATTTACGACATTGAACAGGGCTTCCCTGTTGCGCCGAAAGGTCAATTTATCCTTAAACATCAGCAGTAATCTGGccc  >  1:6036548/1‑93 (MQ=255)

TGTTCTTACCAATGACGATCTGGAATATCGCCACAAAGTCAGCCCGTATGTTGGCCGTACCATTGGCGCGCGTATCACGAAAACCATCTTACGTGGTGATGTGATTTACGACATTGAACAGGGCTTCCCTGTTGCGCCGAAAGGTCAATTTATCCTTAAACATCAGCAGTAATCTGGCCC  >  NC_000913/539561‑539740

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 23 ≤` `ATCG/ATCG` `< 30 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`