BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,035,428	A→G	noncoding (330/2905 nt)	rrlA →	23S ribosomal RNA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,035,428	0	A	G	99.1%	503.7 / NA	210	noncoding (330/2905 nt)	rrlA	23S ribosomal RNA
Reads supporting (aligned to +/- strand): ref base A (0/0); major base G (97/111); minor base C (0/2); total (97/113)
Fisher's exact test for biased strand distribution p-value = 5.01e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 219 total aligned reads displayed.

CAGTAGCGGCGAGCGAACGGGGAGCAGCCCAGAGCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGTGCGATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGAGCTCGATGAGTAGGGCGGGACACGTGGTATCCTGTCTGAATATGGG  >  NC_000913/4035338‑4035506

cAGTAGCGGCGAGCGAACGGGGAGCAGCCCAGAGCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGTGCGATACAGGGTGGCa                                                                              >  1:947052/1‑93 (MQ=18)

    aGCGGCGAGCGAACGGGGAGCAGCCCAGAGCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGTGCGATACAGGGTGGCAGccc                                                                          <  2:6594156/93‑1 (MQ=21)

      cggcgAGCGAACGGGGAGCAGCCCAGAGCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGTGCGATACAGGGTGGCAGCCCCg                                                                        <  1:5071953/93‑1 (MQ=18)

        gcgagcgaACGGGGAGCAGCCCAGAGCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGTGCGATACAGGGTGGCAGCCCCGTa                                                                      >  1:1732907/1‑93 (MQ=18)

         cgagcgaACGGGGAGCAGCCCAGAGCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGTGCGATACAGGGTGGCAGCCCCGTac                                                                     <  1:4245866/93‑1 (MQ=21)

          gagcgaACGGGGAGCAGCCCAGAGCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGTGCGATACAGGGTGGCAGCCCCGTaca                                                                    >  1:5658338/1‑93 (MQ=18)

              gaACGGGGAGCAGCCCAGAGCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGTGCGATACAGGGTGGCAGCCCCGTACACaaa                                                                <  1:5188444/93‑1 (MQ=18)

               aaCGGGGAGCAGCCCAGAGCCTGAATCAGTGTGTGTGTTAGTGGAAGTGTCTGGAAAGGCGTGCGATACAGGGTGGCAGCCCCGTACACaaaa                                                               <  2:4324724/93‑1 (MQ=255)

               aaCGGGGAGCAGCCCAGAGCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGTGCGATACAGGGTGGCAGCCCCGTACACaaaa                                                               >  2:2038734/1‑93 (MQ=18)

               aaCGGGGAGCAGCCCAGAGCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGTGCGATACAGGGTGGCAGCCCCGTACACaaaa                                                               >  2:5668101/1‑93 (MQ=18)

               aaCGGGGAGCAGCCCAGAGCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGTGCGATACAGGGTGGCAGCCCCGGACACaaaa                                                               >  2:436092/1‑93 (MQ=16)

                aCGGGGAGCAGCCCAGAGCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGTGCGATACAGGGTGGCAGCCCCGTACACaaaaa                                                              >  2:3045804/1‑93 (MQ=18)

                aCGGGGAGCAGCCCAGAGCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGTGCGATACAGGGTGGCAGCCCCGCACACaaaaa                                                              >  1:5409763/1‑93 (MQ=16)

                 cGGGGAGCAGCCCAGAGCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGTGCGATACAGGGTGGCAGCCCCGTACACAAAAAt                                                             <  1:1553621/93‑1 (MQ=18)

                  ggggAGCAGCCCAGAGCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGTGCGATACAGGGTGGCAGCCCCGTACACAAAAATg                                                            >  1:4589403/1‑93 (MQ=18)

                   gggAGCAGCCCAGAGCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGTGCGATACAGGGTGGCAGCCCCGTACACAAAAATGc                                                           <  1:3081223/93‑1 (MQ=18)

                   gggAGCAGCCCAGAGCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGTGCGATACAGGGTGGCAGCCCCGTACACAAAAATGc                                                           >  2:5604806/1‑93 (MQ=18)

                     gAGCAGCCCAGAGCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGTGCGATACAGGGTGGCAGCCCCGTACACAAAAATGcac                                                         >  2:635261/1‑93 (MQ=18)

                     gAGCAGCCCAGAGCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGTGCGATACAGGGTGGCAGCCCCGTACACAAAAATGcac                                                         <  2:776115/93‑1 (MQ=18)

                      agcagcCCAGAGCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGTGCGATACAGGGTGGCAGCCCCGTACACAAAAATGcaca                                                        <  1:3826619/93‑1 (MQ=18)

                      agcagcCCAGAGCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGTGCGATACAGGGTGGCAGCCCCGTACACAAAAATGcaca                                                        <  2:799763/93‑1 (MQ=21)

                       gcagcCCAGAGCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGTGCGATACAGGGTGGCAGCCCCGTACACAAAAATGCACAt                                                       <  2:172962/93‑1 (MQ=18)

                       gcagcCCAGAGCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGTGCGATACAGGGTGGCAGCCCCGTACACAAAAATGCACAt                                                       >  2:231312/1‑93 (MQ=18)

                       gcagcCCAGAGCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGTGCGATACAGGGTGGCAGCCCCGTACACAAAAATGCACAt                                                       >  1:1497750/1‑93 (MQ=18)

                       gcagcCCAGAGCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGTGCGATACAGGGTGGCAGCCCCGTACACAAAAATGCACAt                                                       >  1:447553/1‑93 (MQ=18)

                          gcCCAGAGCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGTGCGATACAGGGTGGCAGCCCCGTACACAAAAATGCACATGCt                                                    >  1:5217576/1‑93 (MQ=21)

                          gcCCAGAGCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGTGCGATACAGGGTGGCAGCCCCGTACACAAAAATGCACATGCt                                                    <  1:3302515/93‑1 (MQ=18)

                          gcCCAGAGCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGTGCGATACAGGGTGGCAGCCCCGTACACAAAAATGCACATGCt                                                    <  2:372061/93‑1 (MQ=21)

                           cccAGAGCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGTGCGATACAGGGTGGCAGCCCCGTACACAAAAATGCACATGCtg                                                   <  2:162601/93‑1 (MQ=21)

                            ccAGAGCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGTGCGATACAGGGTGGCAGCCCCGTACACAAAAATGCACATGCtgt                                                  >  1:280290/1‑93 (MQ=18)

                            ccAGAGCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGTGCGATACAGGGTGGCAGCCCCGTACACAAAAATGCACATGCtgt                                                  <  1:4110990/93‑1 (MQ=18)

                            ccAGAGCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGTGCGATACAGGGTGGCAGCCCCGTACACAAAAATGCACATGCtgt                                                  <  1:2479814/93‑1 (MQ=18)

                            ccAGAGCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGTGCGATACAGGGTGGCAGCCCCGTACACAAAAATGCACATGCtgt                                                  >  1:5113546/1‑93 (MQ=18)

                             cAGAGCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGTGCGATACAGGGTGGCAGCCCCGTACACAAAAATGCACATGCtgtg                                                 >  1:5746668/1‑93 (MQ=18)

                              agagCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGTGCGATACAGGGTGGCAGCCCCGTACACAAAAATGCACATGCTGTGa                                                <  2:6035182/93‑1 (MQ=18)

                               gagCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGTGCGATACAGGGTGGCAGCCCCGTACACAAAAATGCACATGCTGTGAg                                               <  2:118039/93‑1 (MQ=21)

                               gagCCTGAATCAGTGTGTGTGTTAGTGCAAGCGTATGGAAAGGCGTGCGAGACAGGGTGCCAGCCCCGTACACAAAAATGCACATGCTGTGAg                                               <  1:1617647/93‑1 (MQ=11)

                                agCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGTGCGATACAGGGTGGCAGCCCCGTACACAAAAATGCACATGCTGTGAGc                                              <  2:1390237/93‑1 (MQ=21)

                                agCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGTGCGATACAGGGTGGCAGCCCCGTACACAAAAATGCACATGCTGTGAGc                                              >  2:6295058/1‑93 (MQ=18)

                                 gCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGTGCGATACAGGGTGGCAGCCCCGTACACAAAAATGCACATGCTGTGAGCt                                             >  2:266898/1‑93 (MQ=18)

                                  ccTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGTGCGATACAGGGTGGCAGCCCCGTACACAAAAATGCACATGCTGTGAGCTc                                            <  2:3225558/93‑1 (MQ=18)

                                  ccTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGTGCGATACAGGGTGGCAGCCCCGTACACAAAAATGCACATGCTGTGAGCTc                                            <  1:3168079/93‑1 (MQ=18)

                                   cTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGTGCGATACAGGGTGGCAGCCCCGTACACAAAAATGCACATGCTGTGAGCTCg                                           >  2:1134758/1‑93 (MQ=18)

                                   cTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGTGCGATACAGGGTGGCAGCCCCGTACACAAAAATGCACATGCTGTGAGCTCg                                           >  1:3860215/1‑93 (MQ=18)

                                    tGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGTGCGATACAGGGTGGCAGCCCCGTACACAAAAATGCACATGCTGTGAGCTcaa                                          <  1:2869841/93‑3 (MQ=16)

                                     gAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGTGCGATACAGGGTGGCAGCCCCGTACACAAAAATGCACATGCTGTGAGCTCGAt                                         >  2:225294/1‑93 (MQ=18)

                                      aaTCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGTGCGATACAGGGTGGCAGCCCCGTACACAAAAATGCACATGCTGTGAGCTCGATg                                        <  1:437/93‑1 (MQ=18)

                                      aaTCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGTGCGATACAGGGTGGCAGCCCCGTACACAAAAATGCACATGCTGTGAGCTCGATg                                        >  2:1349933/1‑93 (MQ=18)

                                       aTCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGTGCGATACAGGGTGGCAGCCCCGTACACAAAAATGCACATGCTGTGAGCTCGATGa                                       >  2:2505171/1‑93 (MQ=18)

                                       aTCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGTGCGATACAGGGTGGCAGCCCCGTACACAAAAATGCACATGCTGTGAGCTCGATGa                                       >  1:1038382/1‑93 (MQ=21)

                                        tCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGTGCGATACAGGGTGGCAGCCCCGTACACAAAAATGCACATGCTGTGAGCTCGATGAg                                      >  1:2196007/1‑93 (MQ=18)

                                        tCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGTGCGATACAGGGTGGCAGCCCCGTACACAAAAATGCACATGCTGTGAGCTCGATGAg                                      >  2:6216668/1‑93 (MQ=18)

                                          ggtgtgtgtgtTAGTGGAAGGGTCTGGAAAGGCGTGCGATACAGGGTGGCAGCCCCGTACACAAAAATGCACATGCTGTGAGCTCGATGAGTa                                    <  2:5811641/92‑1 (MQ=255)

                                          aGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGTGCGATACAGGGTGGCAGCCCCGTACACAAAAATGCACATGCTGTGAGCTCGATGAGTa                                    >  1:1143450/1‑93 (MQ=18)

                                          aGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGTGCGATACAGGGTGGCAGCCCCGTACACAAAAATGCACATGCTGTGAGCTCGATGAGTa                                    <  2:2397682/93‑1 (MQ=18)

                                           gtgtgtgtgtTAGTGGAAGCGTCTGGAAAGGCGTGCGATACAGGGTGGCAGCCCCGTACACAAAAATGCACATGCTGTGAGCTCGATGAGTAg                                   <  2:1234266/93‑1 (MQ=18)

                                           gtgtgtgtgtTAGTGGAAGCGTCTGGAAAGGCGTGCGATACAGGGTGGCAGCCCCGTACACAAAAATGCACATGCTGTGAGCTCGATGAGTAg                                   >  1:4020572/1‑93 (MQ=18)

                                           gtgtgtgtgtTAGTGGAAGCGTCTGGAAAGGCGTGCGATACAGGGTGGCAGCCCCGTACACAAAAATGCACATGCTGTGAGCTCGATGAGTAg                                   >  1:646432/1‑93 (MQ=18)

                                           gtgtgtgtgtTAGTGGAAGCGTCTGGAAAGGCGTGCGATACAGGGTGGCAGCCCCGTACACAAAAATGCACATGCTGTGAGCTCGATGAGTAg                                   >  1:3944541/1‑93 (MQ=18)

                                            tgtgtgtgtTAGTGGAAGCGTCTGGAAAGGCGTGCGATACAGGGTGGCAGCCCCGTACACAAAAATGCACATGCTGTGAGCTCGATGAGTAgg                                  <  2:2577182/93‑1 (MQ=18)

                                             gtgtgtgtTAGTGGAAGCGTCTGGAAAGGCGTGCGATACAGGGTGGCAGCCCCGTACACAAAAATGCACATGCTGTGAGCTCGATGAGTAggg                                 <  2:4015695/93‑1 (MQ=18)

                                              tgtgtgtTAGTGGAAGCGTCTGGAAAGGCGTGCGATACAGGGTGGCAGCCCCGTACACAAAAATGCACATGCTGTGAGCTCGATGAGTAGGGc                                >  2:2972886/1‑93 (MQ=18)

                                                tgtgtTAGTGGAAGCGTCTGGAAAGGCGTGCGATACAGGGTGGCAGCCCCGTACACAAAAATGCACATGCTGTGAGCTCGATTAGTAGGGCgg                              <  2:1053059/93‑1 (MQ=255)

                                                     tAGTGGAAGCGTCTGGAAAGGCGTGCGATACAGGGTGGCAGCCCCGTACACAAAAATGCACATGCTGTGAGCTCGATGAGTAGGGCGGGacac                         <  2:6196930/93‑1 (MQ=18)

                                                       gTGGAAGCGTCTGGAAAGGCGTGCGATACAGGGTGGCAGCCCCGTACACAAAAATGCACATGCTGTGAGCTCGATGAGTAGGGCGGGACACGt                       <  1:2319761/93‑1 (MQ=18)

                                                        tGGAAGCGTCTGGAAAGGCGTGCGATACAGGGTGGCAGCCCCGTACACAAAAATGCACATGCTGTGAGCTCGATGAGTAGGGCGGGACACGTg                      <  2:2098196/93‑1 (MQ=18)

                                                        tGGAAGCGTCTGGAAAGGCGTGCGATACAGGGTGGCAGCCCCGTACACAAAAATGCACATGCTGTGAGCTCGATGAGTAGGGCGGGACACGTg                      >  2:4494511/1‑93 (MQ=18)

                                                        tGGAAGCGTCTGGAAAGGCGTGCGATACAGGGTGGCAGCCCCGTACACAAAAATGCACATGCTGTGAGCTCGATGAGTAGGGCGGGACACGTg                      >  2:4751553/1‑93 (MQ=18)

                                                         ggAAGCGTCTGGAAAGGCGTGCGATACAGGGTGGCAGCCCCGTACACAAAAATGCACNTGNTGTGAGCTCGATGAGTAGGGCGGGACACGTgg                     >  1:5296660/1‑93 (MQ=255)

                                                          gAAGCGTCTGGAAAGGCGTGCGATACAGGGTGGCAGCCCCGTACACAAAAATGCACATGCTGTGAGCTCGATGAGTAGGGCGGGACACGTGGt                    >  2:3864646/1‑93 (MQ=18)

                                                            aGCGTCTGGAAAGGCGTGCGATACAGGGTGGCAGCCCCGTACACAAAAATGCACATGCTGTGAGCTCGATGAGTAGGGCGGGACACGTGGTAt                  >  1:6100640/1‑93 (MQ=21)

                                                             gCGTCTGGAAAGGCGTGCGATACAGGGTGGCAGCCCCGTACACAAAAATGCACATGCTGTGAGCTCGATGAGTAGGGCGGGACACGTGGTATc                 <  2:1827540/93‑1 (MQ=18)

                                                             gCGTCTGGAAAGGCGTGCGATACAGGGTGGCAGCCCCGTACACAAAAATGCACATGCTGTGAGCTCGATGAGTAGGGCGCGACACGTGGTATc                 >  2:3590514/1‑93 (MQ=16)

                                                              cGTCTGGAAAGGCGTGCGATACAGGGTGGCAGCCCCGTACACAAAAATGCACATGCTGTGAGCTCGATGAGTAGGGCGGGACACGTGGTATcc                <  2:4951634/93‑1 (MQ=18)

                                                              cGTCTGGAAAGGCGTGCGATACAGGGTGGCAGCCCCGTACACAAAAATGCACATGCTGTGAGCTCGATGAGTAGGGCGGGACACGTGGTATcc                <  2:3409638/93‑1 (MQ=18)

                                                               gTCTGGAAAGGCGTGCGATACAGGGTGGCAGCCCCGTACACAAAAATGCACATGCTGTGAGCTCGATGAGTAGGGCGGGACACGTGGTATCCt               >  1:1102449/1‑93 (MQ=18)

                                                               gTCTGGAAAGGCGTGCGATACAGGGTGGCAGCCCCGTACACAAAAATGCACATGCTGTGAGCTCGATGAGTAGGGCGGGACACGTGGTATCCt               >  1:2769284/1‑93 (MQ=18)

                                                               gTCTGGAAAGGCGTGCGATACAGGGTGGCAGCCCCGTACACAAAAATGCACATGCTGTGAGCTCGATGAGTAGGGCGGGACACGTGGTATCCt               >  1:1899698/1‑93 (MQ=18)

                                                               gTCTGGAAAGGCGTGCGATACAGGGTGGCAGCCCCGTACACAAAAATGCACATGCTGTGAGCTCGATGAGTAGGGCGGGACACGTGGTATCCt               >  1:167915/1‑93 (MQ=21)

                                                               gTCTGGAAAGGCGTGCGATACAGGGTGGCAGCCCCGTACACAAAAATGCACATGCTGTGAGCTCGATGAGTAGGGCGGGACACGTGGTATCCt               <  1:4708950/93‑1 (MQ=18)

                                                                tCTGGAAAGGCGTGCGATACAGGGTGGCAGCCCCGTACACAAAAATGCACATGCTGTGAGCTCGATGAGTAGGGCGGGACACGTGGTATCCTg              >  2:2016049/1‑93 (MQ=21)

                                                                  tGGAAAGGCGTGCGATACAGGGTGGCAGCCCCGTACACAAAAATGCACATGCTGTGAGCTCGATGAGTAGGGCGGGACACGTGGTATCCTGTc            <  2:1846325/93‑1 (MQ=18)

                                                                  tGGAAAGGCGTGCGATACAGGGTGGCAGCCCCGTACACAAAAATGCACATGCTGTGAGCTCGATGAGTAGGGCGGGACACGTGGTATCCTGTc            <  2:3762118/93‑1 (MQ=18)

                                                                  tGGAAAGGCGTGCGATACAGGGTGGCAGCCCCGTACACAAAAATGCACATGCTGTGAGCTCGATGAGTAGGGCGGGACACGTGGTATCCTGTc            >  1:3432974/1‑93 (MQ=18)

                                                                   ggAAAGGCGTGCGATACAGGGTGGCAGCCCCGTACACAAAAATGCACATGCTGTGAGCTCGATGAGTAGGGCGGGACACGTGGTATCCTGTCt           >  2:191097/1‑93 (MQ=21)

                                                                   ggAAAGGCGTGCGATACAGGGTGGCAGCCCCGTACACAAAAATGCACATGCTGTGAGCTCGATGAGTAGGGCGGGACACGTGGTATCCTGTCt           >  2:2740349/1‑93 (MQ=18)

                                                                   ggAAAGGCGTGCGATACAGGGTGGCAGCCCCGTACACAAAAATGCACATGCTGTGAGCTCGATGAGTAGGGCGGGACACGTGGTATCCTGTCt           >  2:6252100/1‑93 (MQ=18)

                                                                    gAAAGGCGTGCGATACAGGGTGGCAGCCCCGTACACAAAAATGCACATGCTGTGAGCTCGATGAGTAGGGCGGGACACGTGGTATCCTGTCTg          >  1:5785265/1‑93 (MQ=18)

                                                                    gAAAGGCGTGCGATACAGGGTGGCAGCCCCGTACACAAAAATGCACATACTGTGAGCTCGATGAGTAGGGCGGGACACGTGGTATCCTGTCTg          >  2:390819/1‑93 (MQ=16)

                                                                       aGGCGTGCGATGCAGAGTGGCAGCCCCGTACACAAAAATGCACATGCTGTGAGCTCGATGAGTAGGGCGGGACACGTGGTATCCTGTCTGAat       <  2:5956921/93‑1 (MQ=255)

                                                                       aGGCGTGCGATACAGGGTGGCAGCCCCGTACACAAAAATGCACATGCTGTGAGCTCGATGAGTAGGGCGGGACACGTGGTATCCTGTCTGAat       <  1:6686362/93‑1 (MQ=18)

                                                                       aGGCGTGCGATACAGGGTGGCAGCCCCGTACACAAAAATGCACATGCTGTGAGCTCGATGAGTAGGGCGGGACACGTGGTATCCTGTCTGAat       >  2:1465051/1‑93 (MQ=21)

                                                                        ggCGTGCGATACAGGGTGGCAGCCCCGTACACAAAAATGCACATGCTGTGAGCTCGATGAGTAGGGCGGGACACGTGGTATCCTGTCTGAata      >  1:2977298/1‑93 (MQ=18)

                                                                        ggCGTGCGATACAGGGTGGCAGCCCCGTACACAAAAATGCACATGCTGTGAGCTCGATGAGTAGGGCGGGACACGTGGTATCCTGTCTGAata      <  2:5453032/93‑1 (MQ=18)

                                                                        ggCGTGCGATACAGGGTGGCAGCCCCGTACACAAAAATGCACATGCTGTGAGCTCGATGAGTAGGGCGGGACACGTGGTATCCTGTCTGAata      <  1:4176345/93‑1 (MQ=18)

                                                                         gCGTGCGATACAGGGTGGCAGCCCCGTACACAAAAATGCACATGCTGTGAGCTCGATGAGTAGGGCGGGACACGTGGTATCCTGTCTGAatat     <  2:6533957/93‑1 (MQ=18)

                                                                           gTGCGATACAGGGTGGCAGCCCCGTACACAAAAATGCACATGCTGTGAGCTCGATGAGTAGGGCGGGACACGTGGTATCCTGTCTGAATATgg   >  2:4600059/1‑93 (MQ=17)

                                                                            tGCGATACAGGGTGGCAGCCCCGTACACAAAAATGCACATGCTGTGAGCTCGATGAGTAGGGCGGGACACGTGGTATCCTGTCTGAATATggg  >  1:816616/1‑93 (MQ=14)

                                                                            tGCGATACAGGGTGGCAGCCCCGTACACAAAAATGCACATGCTGTGAGCTCGATGAGTAGGGCGGGACACGTGGTATCCTGTCTGAATATggg  <  1:3259625/93‑1 (MQ=14)

                                                                            tGCGATACAGGGTGGCAGCCCCGTACACAAAAATGCACATGCTGTGAGCTCGATGAGTAGGGCGGGACACGTGGTATCCTGTCTGAATATggg  <  1:1285108/93‑1 (MQ=14)

CAGTAGCGGCGAGCGAACGGGGAGCAGCCCAGAGCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGTGCGATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGAGCTCGATGAGTAGGGCGGGACACGTGGTATCCTGTCTGAATATGGG  >  NC_000913/4035338‑4035506

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 20 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`