Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,060,112 A→G Y96C (TAT→TGT)  yihN → putative resistance protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,060,1120AG99.6% 868.7 / ‑4.8 273Y96C (TAT→TGT) yihNputative resistance protein
Reads supporting (aligned to +/- strand):  ref base A (0/1);  new base G (134/138);  total (134/139)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.62e-01

Only 100 of 278 total aligned reads displayed.

TTGCCGATAAACTACCGCGCAGATACACCATGTCATTCTCACTCATTGCGACAGGATTACTGGGTGTTTATTTGACGACAATGCCGGGGTATTGGGGCATCCTCTTTGTCTGGGCGCTATTTGGCGTTACTTGCGACATGATGAACTGGCCGGTCTTGCTCAAGTCGGTAAGTCGATTGG  >  NC_000913/4060022‑4060201
                                                                                          |                                                                                         
ttGCCGATAAACTACCGCGCAGATACACCATGTCATTCTCACTCATTGCGACAGGATTACTGGGTGTTTATTTGACGACAATGCCGGGGTGtt                                                                                         >  1:4250797/1‑93 (MQ=255)
 tGCCGATAAACTACCGCGCAGATACACCATGTCATTCTCACTCATTGCGACAGGATTACTGGGTGTTTATTTGACGACAATGCCGGGGTGTTg                                                                                        <  1:4169784/93‑1 (MQ=255)
 tGCCGATAAACTACCGCGCAGATACACCATGTCATTCTCACTCATTGCGACAGGATTACTGGGTGTTTATTTGACGACAATGCCGGGGTGTTg                                                                                        <  1:6225816/93‑1 (MQ=255)
  gCCGATAAACTACCGCGCAGATACACCATGTCATTCTCACTCATTGCGACAGGATTACTGGGTGTTTATTTGACGACAATGCCGGGGTGTTgg                                                                                       <  1:4864091/93‑1 (MQ=255)
  gCCGATAAACTACCGCGCAGATACACCATGTCATTCTCACTCATTGCGACAGGATTACTGGGTGTTTATTTGACGACAATGCCGGGGTGTTgg                                                                                       <  2:5251526/93‑1 (MQ=255)
   ccGATAAACTACCGCGCAGATACACCATGTCATTCTCACTCATTGCGACAGGATTACTGGGTGTTTATTTGACGACAATGCCGGGGTGTTggg                                                                                      <  2:5552566/93‑1 (MQ=255)
   ccGATAAACTACCGCGCAGATACACCATGTCATTCTCACTCATTGCGACAGGATTACTGGGTGTTTATTTGACGACAATGCCGGGGTGTTggg                                                                                      >  2:22914/1‑93 (MQ=255)
   ccGATAAACTACCGCGCAGATACACCATGTCATTCTCACTCATTGCGACAGGATTACTGGGTGTTTATTTGACGACAATGCCGGGGTGTTggg                                                                                      <  1:2288750/93‑1 (MQ=255)
    cGATAAACTACCGCGCAGATACACCATGTCATTCTCACTCATTGCGACAGGATTACTGGGTGTTTATTTGACGACAATGCCGGGGTGTTgggg                                                                                     >  1:2905722/1‑93 (MQ=255)
     gATAAACTACCGCGCAGATACACCATGTCATTCTCACTCATTGCGACAGGATTACTGGGTGTTTATTTGACGACAATGCCGGGGTGTTGGGGc                                                                                    >  2:4352386/1‑93 (MQ=255)
     gATAAACTACCGCGCAGATACACCATGTCATTCTCACTCATTGCGACAGGATTACTGGGTGTTTATTTGACGACAATGCCGGGGTGTTGGGGc                                                                                    >  2:5733852/1‑93 (MQ=255)
     gATAAACTACCGCGCAGATACACCATGTCATTCTCACTCATTGCGACAGGATTACTGGGTGTTTATTTGACGACAATGCCGGGGTGTTGGGGc                                                                                    >  1:2181897/1‑93 (MQ=255)
       tAAACTACCGCGCAGATACACCATGTCATTCTCACTCATTGCGACAGGATTACTGGGTGTTTATTTGACGACAATGCCGGGGTGTTGGGGCAt                                                                                  >  1:6154662/1‑93 (MQ=255)
         aaCTACCGCGCAGATACACCATGTCATTCTCACTCATTGCGACAGGATTACTGGGTGTTTATTTGACGACAATGCCGGGGTGTTGGGGCATcc                                                                                >  2:3974494/1‑93 (MQ=255)
         aaCTACCGCGCAGATACACCATGTCATTCTCACTCATTGCGACAGGATTACTGGGTGTTTATTTGACGACAATGCCGGGGTGTTGGGGCATcc                                                                                >  2:5367844/1‑93 (MQ=255)
         aaCTACCGCGCAGATACACCATGTCATTCTCACTCATTGCGACAGGATTACTGGGTGTTTATTTGACGACAATGCCGGGGTGTTGGGGCATcc                                                                                >  1:1476290/1‑93 (MQ=255)
         aaCTACCGCGCAGATACACCATGTCATTCTCACTCATTGCGACAGGATTACTGGGTGTTTATTTGACGACAATGCCGGGGTGTTGGGGCATcc                                                                                <  2:6388335/93‑1 (MQ=255)
         aaCTACCGCGCAGATACACCATGTCATTCTCACTCATTGCGACAGGATTACTGGGTGTTTATTTGACGACAATGCCGGGGTGTTGGGGCATcc                                                                                >  1:5567295/1‑93 (MQ=255)
           cTACCGCGCAGATACACCATGTCATTCTCACTCATTGCGACAGGATTACTGGGTGTTTATTTGACGACAATGCCGGGGTGTTGGTGCATCctc                                                                              <  1:123773/93‑1 (MQ=255)
           cTACCGCGCAGATACACCATGTCATTCTCACTCATTGCGACAGGATTACTGGGTGTTTATTTGACGACAATGCCGGGGTGTTGGGGCATCctc                                                                              <  2:1928108/93‑1 (MQ=255)
              ccGCGCAGATACACCATGTCATTCTCACTCATTGCGACAGGATTACTGGGTGTTTATTTGACGACAATGCCGGGGTGTTGGGGCATCCTCttt                                                                           <  1:2232064/93‑1 (MQ=255)
               cgcgCAGATACACCATGTCATTCTCACTCATTGCGACAGGATTACTGGGTGTTTATTTGACGACAATGCCGGGGTGTTGGGGCATCCTCTTTg                                                                          >  2:3827092/1‑93 (MQ=255)
                gcgcAGATACACCATGTCATTCTCACTCATTGCGACAGGATTACTGGGTGTTTATTTGACGACAATGCCGGGGTGTTGGGGCATCCTCTTTGt                                                                         >  1:3380484/1‑93 (MQ=255)
                gcgcAGATACACCATGTCATTCTCACTCATTGCGACAGGATTACTGGGTGTTTATTTGACGACAATGCCGGGGTGTTGGGGCATCCTCTTTGt                                                                         >  2:1227295/1‑93 (MQ=255)
                   cAGATACACCATGTCATTCTCACTCATTGCGACAGGATTACTGGGTGTTTATTTGACGACAATGCCGGGGTGTTGGGGCATCCTCTTTGTCTg                                                                      >  2:5911406/1‑93 (MQ=255)
                     gATACACCATGTCATTCTCACTCATTGCGACAGGATTACTGGGTGTTTATTTGACGACAATGCCGGGGTGTTGGGGCATCCTCTTTGTCTggg                                                                    >  1:3031038/1‑93 (MQ=255)
                     gATACACCATGTCATTCTCACTCATTGCGACAGGATTACTGGGTGTTTATTTGACGACAATGCCGGGGTGTTGGGGCATCCTCTTTGTCTggg                                                                    <  2:2461765/93‑1 (MQ=255)
                      attcacCATGTCATTCTCACTCATTGCGACAGGATTACTGGGTGTTTATTTGACGACAATGCCGGGGTGTTGGGGCATCCTCTTTGTCTGGgc                                                                   <  2:6129056/90‑1 (MQ=255)
                      aTACACCATGTCATTCTCACTCATTGCGACAGGATTACTGGGTGTTTATTTGACGACAATGCCGGGGTGTTGGGGCATCCTCTTTGTCTGGgc                                                                   <  2:4442858/93‑1 (MQ=255)
                       tACACCATGTCATTCTCACTCATTGCGACAGGATTACTGGGTGTTTATTTGACGACAATGCCGGGGTGTTGGGGCATCCTCTTTGTCTGGgcg                                                                  >  2:160200/1‑93 (MQ=255)
                       tACACCATGTCATTCTCACTCATTGCGACAGGATTACTGGGTGTTTATTTGACGACAATGCCGGGGTGTTGGGGCATCCTCTTTGTCTGGgcg                                                                  >  2:6556143/1‑93 (MQ=255)
                       tACACCATGTCATTCTCACTCATTGCGACAGGATTACTGGGTGTTTATTTGACGACAATGCCGGGGTGTTGGGGCATCCTCTTTGTCTGGgcg                                                                  >  1:2570321/1‑93 (MQ=255)
                       tACACCATGTCATTCTCACTCATTGCGACAGGATTACTGGGTGTTTATTTGACGACAATGCCGGGGTGTTGGGGCATCCTCTTTGTCTGGgcg                                                                  >  1:4356658/1‑93 (MQ=255)
                        acacCATGTCATTCTCACTCATTGCGACAGGATTACTGGGTGTTTATTTGACGACAATGCCGGGGTGTTGGGGCATCCTCTTTGTCTGGgcgc                                                                 >  1:1149541/1‑93 (MQ=255)
                         cacCATGTCATTCTCACTCATTGCGACAGGATTACTGGGTGTTTATTTGACGACAATGCCGGGGTGTTGGGGCATCCTCTTTGTCTGGGCGCt                                                                <  2:4746877/93‑1 (MQ=255)
                           ccATGTCATTCTCACTCATTGCGACAGGATTACTGGGTGTTTATTTGACGACAATGCCGGGGTGTTGGGGCATCCTCTTTGTCTGGGCGCTAt                                                              <  1:1696204/93‑1 (MQ=255)
                             aTGTCATTCTCACTCATTGCGACAGGATTACTGGGTGTTTATTTGACGACAATGCCGGGGTGTTGGGGCATCCTCTTTGTCTGGGCGCTAttt                                                            <  1:2441997/93‑1 (MQ=255)
                             aTGTCATTCTCACTCATTGCGACAGGATTACTGGGTGTTTATTTGACGACAATGCCGGGGTGTTGGGGCATCCTCTTTGTCTGGGCGCTAttt                                                            >  1:3213492/1‑93 (MQ=255)
                             aTGTCATTCTCACTCATTGCGACAGGATTACTGGGTGTTTATTTGACGACAATGCCGGGGTGTTGGGGCATCCTCTTTGTCTGGGCGCTAttt                                                            <  2:5847338/93‑1 (MQ=255)
                              tGTCATTCTCACTCATTGCGACAGGATTACTGGGTGTTTATTTGACGACAATGCCGGGGTGTTGGGGCATCCTCTTTGTCTGGGCGCTATTTg                                                           >  2:4032912/1‑93 (MQ=255)
                                tCATTCTCACTCATTGCGACAGGATTACTGGGTGTTTATTTGACGACAATGCCGGGGTGTTGGGGCATCCTCTTTGTCTGGGCGCTATTTGGc                                                         <  1:2693905/93‑1 (MQ=255)
                                tCATTCTCACTCATTGCGACAGGATTACTGGGTGTTTATTTGACGACAATGCCGGGGTGTTGGGGCATCCTCTTTGTCTGGGCGCTATTTGGc                                                         >  1:5998545/1‑93 (MQ=255)
                                 cATTCTCACTCATTGCGACAGGATTACTGGGTGTTTATTTGACGACAATGCCGGGGTGTTGGGGCATCCTCTTTGTCTGGGCGCTATTTGGCg                                                        >  2:3800450/1‑93 (MQ=255)
                                  aTTCTCGCTCATTGCGACAGGATTACTGGGTGTTTATTTGACGACAATGCCGGGGTGTTGGGGCATCCTCTTTGTCTGGGCGCTATTTGGCGt                                                       <  2:1306292/93‑1 (MQ=255)
                                  aTTCTCACTCATTGCGACAGGATTACTGGGTGTTTATTTGACGACAATGCCGGGGTGTTGGGGCATCCTCTTTGTCTGGGCGCTATTTGGCGt                                                       <  1:904708/93‑1 (MQ=255)
                                  aTTCTCACTCATTGCGACAGGATTACTGGGTGTTTATTTGACGACAATGCCGGGGTGTTGGGGCATCCTCTTTGTCTGGGCGCTATTTGGCGt                                                       <  2:1514027/93‑1 (MQ=255)
                                   ttCTCACTCATTGCGACAGGATTACTGGGTGTTTATTTGACGACAATGCCGGGGTGTTGGGGCATCCTCTTTGTCTGGGCGCTATTTGGCgct                                                      >  2:3114525/1‑91 (MQ=255)
                                   ttCTCACTCATTGCGACAGGATTACTGGGTGTTTATTTGACGACAATGCCGGGGTGTTGGGGCATCCTCTTTGTCTGGGCGCTATTTGGCGtt                                                      <  2:2587460/93‑1 (MQ=255)
                                   ttCTCACTCATTGCGACAGGATTACTGGGTGTTTATTTGACGACAATGCCGGGGTGTTGGGGCATCCTCTTTGTCTGGGCGCTATTTGGCGtt                                                      <  1:3929926/93‑1 (MQ=255)
                                    tctcACTCATTGCGACAGGATTACTGGGTGTTTATTTGACGACAATGCCGGGGTGTTGGGGCATCCTCTTTGTCTGGGCGCTATTTGGCGTTa                                                     >  2:2349339/1‑93 (MQ=255)
                                     ctcactcaTTGCGACAGGATTACTGGGTGTTTATTTGACGACAATGCCGGGGTGTTGGGGCATCCTCTTTGTCTGGGCGCTATTTGGCGTTAc                                                    >  1:3438844/1‑93 (MQ=255)
                                      tcactcaTTGCGACAGGATTACTGGGTGTTTATTTGACGACAATGCCGGGGTGTTGGGGCATCCTCTTTGTCTGGGCGCTATTTGGCGTTACt                                                   <  2:3279111/93‑1 (MQ=255)
                                      tcactcaTTGCGACAGGATTACTGGGTGTTTATTTGACGACAATGCCGGGGTGTTGGGGCATCCTCTTTGTCTGGGCGCTATTTGGCGTTACt                                                   >  1:5523585/1‑93 (MQ=255)
                                         ctcaTTGCGACAGGATTACTGGGTGTTTATTTGACGACAATGCCGGGGTGTTGGGGCATCCTCTTTGTCTGGGCGCTATTTGGCGTTACTTGc                                                >  2:3237163/1‑93 (MQ=255)
                                          tcaTTGCGACAGGATTACTGGGTGTTTATTTGACGACAATGCCGGGGTGTTGGGGCATCCTCTTTGTCTGGGCGCTATTTGGCGTTACTTGCg                                               >  2:3171251/1‑93 (MQ=255)
                                          tcaTTGCGACAGGATTACTGGGTGTTTATTTGACGACAATGCCGGGGTGTTGGGGCATCCTCTTTGTCTGGGCGCTATTTGGCGTTACTTGCg                                               >  1:6327728/1‑93 (MQ=255)
                                          tcaTTGCGACAGGATTACTGGGTGTTTATTTGACGACAATGCCGGGGTGTTGGGGCATCCTCTTTGTCTGGGCGCTATTTGGCGTTACTTGCg                                               >  2:4975848/1‑93 (MQ=255)
                                           caTTGCGCCAGGATTACTGGGTGTTTATTTGACGACAATGCCGGGGTGTTGGGGCATCCTCTTTGTCTGGGCGCTATTTGGCGTTACTTGCGa                                              >  1:830187/1‑93 (MQ=255)
                                            aTTGCGACAGGATTACTGGGTGTTTATTTGACGACAATGCCGGGGTGTTGGGGCATCCTCTTTGTCTGGGCGCTATTTGGCGTTACTTGCGAc                                             >  2:2160859/1‑93 (MQ=255)
                                            aTTGCGACAGGATTACTGGGTGTTTATTTGACGACAATGCCGGGGTGTTGGGGCATCCTCTTTGTCTGGGCGCTATTTGGCGTTACTTGCGAc                                             >  2:869054/1‑93 (MQ=255)
                                             ttGCGACAGGATTACTGGGTGTTTATTTGACGACAATGCCGGGGTGTTGGGGCATCCTCTTTGTCTGGGCGCTATTTGGCGTTACTTGCGACa                                            >  1:409881/1‑93 (MQ=255)
                                                 gACAGGATTACTGGGTGTTTATTTGACGACAATGCCGGGGTGTTGGGGCATCCTCTTTGTCTGGGCGCTATTTGGCGTTACTTGCGACatgat                                        <  1:5481233/93‑1 (MQ=255)
                                                 gACAGGATTACTGGGTGTTTATTTGACGACAATGCCGGGGTGTTGGGGCATCCTCTTTGTCTGGGCGCTATTTGGCGTTACTTGCGACatgat                                        <  2:3351001/93‑1 (MQ=255)
                                                  aCAGGATTACTGGGTGTTTATTTGACGACAATGCCGGGGTGTTGGGGCATCCTCTTTGTCTGGGCGCTATTTGGCGTTACTTGCGACatgatg                                       >  2:4578789/1‑93 (MQ=255)
                                                   cAGGATTACTGGGTGTTTATTTGACGACAATGCCGGGGTGTTGGGGCATCCTCTTTGTCTGGGCGCTATTTGGCGTTACTTGCGACAtgatga                                      <  2:4875360/93‑1 (MQ=255)
                                                    aGGATTACTGGGTGTTTATTTGACGACAATGCCGGGGTGTTGGGGCATCCTCTTTGTCTGGGCGCTATTTGGCGTTACTTGCGACATGATGaa                                     <  1:6700554/93‑1 (MQ=255)
                                                    aGGATTACTGGGTGTTTATTTGACGACAATGCCGGGGTGTTGGGGCATCCTCTTTGTCTGGGCGCTATTTGGCGTTACTTGCGACATGATGaa                                     >  1:1359842/1‑93 (MQ=255)
                                                    aGGATTACTGGGGGTTTATTTGACGACAATGCCGGGGTGTTGGGGCATCCTCTTTGTCTGGGCGCTATTTGGTGTTACTTGCGACATGATGaa                                     >  2:4133383/1‑93 (MQ=255)
                                                     ggATTACTGGGTGTTTATTTGACGACAATGCCGGGGTGTTGGGGCATCCTCTTTGTCTGGGCGCTATTTGGCGTTACTTGCGACATGATGAAc                                    >  1:3632677/1‑93 (MQ=255)
                                                      gATTACTGGGAGTTTATTTGACGACAATGCCGGGGGGGTGGGGCATCCTCTTTGTCTGGGCGCTATTTGGCGTTACTTGCGACATGATGAACt                                   >  1:2840176/1‑93 (MQ=255)
                                                        ttACTGGGTGTTTATTTGACGACAATGCCGGGGTGTTGGGGCATCCTCTTTGTCTGGGCGCTATTTGGCGTTACTTGCGACATGATGAACTgg                                 >  1:5631235/1‑93 (MQ=255)
                                                          aCTGGGTGTTTATTTGACGACAATGCCGGGGTGTTGGGGCATCCTCTTTGTCTGGGCGCTATTTGGCGTTACTTGCGACATGATGAACTGGcc                               <  2:1353756/93‑1 (MQ=255)
                                                          aCTGGGTGTTTATTTGACGACAATGCCGGGGTGTTGGGGCATCCTCTTTGTCTGGGCGCTATTTGGCGTTACTTGCGACATGATGAACTGGcc                               >  1:1313078/1‑93 (MQ=255)
                                                           cTGGGTGTTTATTTGACGACAATGCCGGGGTGTTGGGGCATCCTCTTTGTCTGGGCGCTATTTGGCGTTACTTGCGACATGATGAACTGGCCg                              >  2:5656608/1‑93 (MQ=255)
                                                           cTGGGTGTTTATTTGACGACAATGCCGGGGTGTTGGGGCATCCTCTTTGTCTGGGCGCTATTTGGCGTTACTTGCGACATGATGAACTGGCCg                              >  2:681979/1‑93 (MQ=255)
                                                            tGGGTGTTTATTTGACGACAATGCCGGGGTGTTGGGGCATCCTCTTTGTCTGGGCGCTATTTGGCGTTACTTGCGACATGATGAAGTGGCCgg                             >  1:4647122/1‑93 (MQ=255)
                                                            tGGGTGTTTATTTGACGACAATGCCGGGGTGTTGGGGCATCCTCTTTGTCTGGGCGCTATTTGGCGTTACTTGCGACATGATGAACTGGCCgg                             <  2:3591845/93‑1 (MQ=255)
                                                            tGGGAGTTTATTTGACGACAATGCCGGGGAGTTGGGGCATCCTCTTTGTCTGGGCGCTATTTGGCGTTACTTGCGACATGATGAACTGGCCgg                             >  1:4739352/1‑93 (MQ=255)
                                                             gggTGTTTATTTGACGACAATGCCGGGGTGTTGGGGCATCCTCTTTGTCTGGGCGCTATTTGGCGTTACTTGCGACATGATGAACTGGCCGGt                            >  1:658092/1‑93 (MQ=255)
                                                              ggTGTTTATTTGACGACAATGCCGGGGTGTTGGGGCATCCTCTTTGTCTGGGCGCTATTTGGCGTTACTTGCGACATGATGAACTGGCCGGTc                           <  1:1905011/93‑1 (MQ=255)
                                                               gtgtTTATTTGACGACAATGCCGGGGTGTTGGGGCATCCTCTTTGTCTGGGCGCTATTTGGCGTTACTTGCGACATGATGAACTGGCCGGTCt                          >  1:4971055/1‑93 (MQ=255)
                                                               gtgtTTATTTGACGACAATGCCGGGGTGTTGGGGCATCCTCTTTGTCTGGGCGCTATTTGGCGTTACTTGCGACATGATGAACTGGCCGGTCt                          <  2:6209020/93‑1 (MQ=255)
                                                               gtgtTTATTTGACGACAATGCCGGGGTGTTGGGGCATCCTCTTTGTCTGGGCGCTATTTGGCGTTACTTGCGACATGATGAACTGGCCGGTCt                          <  2:3862343/93‑1 (MQ=255)
                                                                tgtTTATTTGACGACAATGCCGGGGTGTTGGGGCATCCTCTTTGTCTGGGCGCTATTTGGCGTTACTTGCGACATGATGAACTGCCCGGTCtt                         <  1:6611026/93‑1 (MQ=255)
                                                                  tttATTTGACGACAATGCCGGGGTGTTGGGGCATCCTCTTTGTCTGGGCGCTATTTGGCGTTACTTGCGACATGATGAACTGGCCGGTCTTGc                       <  1:5367946/93‑1 (MQ=255)
                                                                    tATTTGACGACAATGCCGGGGTGTTGGGGCATCCTCTTTGTCTGGGCGCTATTTGGCGTTACTTGCGACATGATGAACTGGCCGGTCTTGCTc                     <  2:2595646/93‑1 (MQ=255)
                                                                      tttGACGACAATGCCGGGGTGTTGGGGCATCCTCTTTGTCTGGGCGCTATTTGGCGTTACTTGCGACATGATGAACTGGCCGGTCTTGCTCaa                   >  2:5614371/1‑93 (MQ=255)
                                                                         gacgacAATGCCGGGGTGTTGGGGCATCCTCTTTGTCTGGGCGCTATTTGGCGTTACTTGCGACATGATGAACTGGCCGGTCTTGCTCAAGTc                <  2:1074658/93‑1 (MQ=255)
                                                                         gacgacAATGCCGGGGTGTTGGGGCATCCTCTTTGTCTGGGCGCTATTTGGCGTTACTTGCGACATGATGAACTGGCCGGTCTTGCTCAAGTc                <  1:3795498/93‑1 (MQ=255)
                                                                         gacgacAATGCCGGGGTGTTGGGGCATCCTCTTTGTCTGGGCGCTATTTGGCGTTACTTGCGACATGATGAACTGGCCGGTCTTGCTCAAGTc                >  1:4023194/1‑93 (MQ=255)
                                                                           cgacAATGCCGGGGTGTTGGGGCATCCTCTTTGTCTGGGCGCTATTTGGCGTTACTTGCGACATGATGAACTGGCCGGTCTTGCTCAAGTCgg              >  2:5127603/1‑93 (MQ=255)
                                                                                aTGCCGGGGTGTTGGGGCATCCTCTTTGTCTGGGCGCTATTTGGCGTTACTTGCGACATGATGAACTGGCCGGTCTTGCTCAAGTCGGTAAGt         >  2:4489168/1‑93 (MQ=255)
                                                                                  gCCGGGGTGTTGGGGCATCCTCTTTGTCTGGGCGCTATTTGGCGTTACTTGCGACATGATGAACTGGCCGGTCTTGCTCAAGTCGGTAAGTCg       <  1:4564129/93‑1 (MQ=255)
                                                                                  gCCGGGGTGTTGGGGCATCCGCTTTGTCTGGGCGCTATTTGGCGTTACTTGCGACATGATGAACTGGCCGGTCTTGCTCAAGTCGGTAAGTCg       >  1:6455521/1‑93 (MQ=255)
                                                                                   ccGGGGTGTTGGGGCATCCTCTTTGTCTGGGCGCTATTTGGCGTTACTTGCGACATGATGAACTGGCCGGTCTTGCTCAAGTCGGTAAGTCGa      <  1:159089/93‑1 (MQ=255)
                                                                                    cGGGGTGTTGGGGCATCCTCTTTGTCTGGGCGCTATTTGGCGTTACTTGCGACATGATGAACTGGCCGGTCTTGCTCAAGTCGGTATGTCGAt     >  1:5735475/1‑93 (MQ=255)
                                                                                      gggTGTTGGGGCATCCTCTTTGTCTGGGCGCTATTTGGCGTTACTTGCGACATGATGAACTGGCCGGTCTTGCTCAAGTCGGTAAGTCGATTg   <  1:38304/93‑1 (MQ=255)
                                                                                      gggTGTTGGGGCATCCTCTTTGTCTGGGCGCTATTTGGCGTTACTTGCGACATGATGAACTGGCCGGTCTTGCTCAAGTCGGTAAGTCGATTg   <  2:780981/93‑1 (MQ=255)
                                                                                       ggTGTTGGGGCATCCTCTTTGTCTGGGCGCTATTTGGCGTTACTTGCGACATGATGAACTGGCCGGTCTTGCTCAAGTCGGTAAGTCGATTgg  >  1:5807484/1‑93 (MQ=255)
                                                                                       ggTGTTGGGGCATCCTCTTTGTCTGGGCGCTATTTGGCGTTACTTGCGACATGATGAACTGGCCGGTCTTGCTCAAGTCGGTAAGTCGATTgg  <  1:4300343/93‑1 (MQ=255)
                                                                                          |                                                                                         
TTGCCGATAAACTACCGCGCAGATACACCATGTCATTCTCACTCATTGCGACAGGATTACTGGGTGTTTATTTGACGACAATGCCGGGGTATTGGGGCATCCTCTTTGTCTGGGCGCTATTTGGCGTTACTTGCGACATGATGAACTGGCCGGTCTTGCTCAAGTCGGTAAGTCGATTGG  >  NC_000913/4060022‑4060201

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑