BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,149,444	A→G	Y412H (TAC→CAC)	ppc ←	phosphoenolpyruvate carboxylase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,149,444	0	A	G	100.0%	785.1 / NA	245	Y412H (TAC→CAC)	ppc	phosphoenolpyruvate carboxylase
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (130/115); total (130/115)

Only 100 of 253 total aligned reads displayed.

GCAGAAGCGGACGTTTGGAGTTCAGTTCGCGGATCAGGAACGCCTGTTTGTCGGCCTCTGACCAGCTTTCGTAGTCGCCGATACCGAGGTAGCGGGTCAGCTCGCCCAGCGCTTCGGTATGACGCGTGCTCTCCTGACGGATATCAATACGGACCAGCGGTACGCCGAAACATTTCACGC  >  NC_000913/4149354‑4149533

 cAGAAGCGGACGTTTGGAGTTCAGTTCGCGGATCAGGAACGCCTGTTTGTCGGCCTCTGACCAGCTTTCGTAGTCGCCGATACCGAGGTGGCg                                                                                        >  2:3217311/1‑93 (MQ=255)

  aGAAGCGGACGTTTGGAGTTCAGTTCGCGGATCAGGAACGCCTGTTTGTCGGCCTCTGACCAGCTTTCGTAGTCGCCGATACCGAGGTGGCgg                                                                                       <  2:5063960/93‑1 (MQ=255)

   gAAGCGGACGTTTGGAGTTCAGTTCGCGGATCAGGAACGCCTGTTTGTCGGCCTCTGACCAGCTTTCGTAGTCGCCGATACCGAGGTGGCggg                                                                                      >  1:583136/1‑93 (MQ=255)

    aaGCGGACGTTTGGAGTTCAGTTCGCGGATCAGGAACGCCTGTTTGTCGGCCTCTGACCAGCTTTCGTAGTCGCCGATACCGAGGTGGCGGGt                                                                                     <  2:1159684/93‑1 (MQ=255)

     aGCGGACGTTTGGAGTTCAGTTCGCGGATCAGGAACGCCTGTTTGTCGGCCTCTGACCAGCTTTCGTAGTCGCCGATACCGAGGTGGCGGGTc                                                                                    >  2:1419600/1‑93 (MQ=255)

     aGCGGACGTTTGGAGTTCAGTTCGCGGATCAGGAACGCCTGTTTGTCGGCCTCTGACCAGCTTTCGTAGTCGCCGATACCGAGGTGGCGGGTc                                                                                    <  2:5431415/93‑1 (MQ=255)

     aGCGGACGTTTGGAGTTCAGTTCGCGGATCAGGAACGCCTGTTTGTCGGCCTCTGACCAGCTTTCGTAGTCGCCGATACCGAGGTGGCGGGTc                                                                                    <  2:293575/93‑1 (MQ=255)

     aGCGGACGTTTGGAGTTCAGTTCGCGGATCAGGAACGCCTGTTTGTCGGCCTCTGACCAGCTTTCGTAGTCGCCGATACCGAGGTGGCGGGTc                                                                                    <  2:5520847/93‑1 (MQ=255)

     aGCGGACGTTTGGAGTTCAGTTCGCGGATCAGGAACGCCTGTTTGTCGGCCTCTGACCAGCTATCGTAGTCGCCGATACCGAGGTGGCGGCTc                                                                                    >  2:2726142/1‑93 (MQ=255)

       cGGACGTTTGGAGTTCAGTTCGCGGATCAGGAACGCCTGTTTGTCGGCCTCTGACCAGCTTTCGTAGTCGCCGATACCGAGGTGGCGGGTCAg                                                                                  >  1:6032065/1‑93 (MQ=255)

        ggACGTTTGGAGTTCAGTTCGCGGATCAGGAACGCCTGTTTGTCGGCCTCTGACCAGCTTTCGTAGTCGCCGATACCGAGGTGGCGGGTCAGc                                                                                 >  1:6595490/1‑93 (MQ=255)

         gACGTTTGGAGTTCAGTTCGCGGATCAGGAACGCCTGTTTGTCGGCCTCTGACCAGCTTTCGTAGTCGCCGATACCGAGGTGGCGGGTCAGCt                                                                                >  1:6194346/1‑93 (MQ=255)

         gACGTTTGGAGTTCAGTTCGCGGATCAGGAACGCCTGTTTGTCGGCCTCTGACCAGCTTTCGTAGTCGCCGATACCGAGGTGGCGGGTCAGCt                                                                                <  1:5382489/93‑1 (MQ=255)

         gACGTTTGGAGTTCAGTTCGCGGATCAGGAACGCCTGTTTGTCGGCCTCTGACCAGCTTTCGTAGTCGCCGATACCGAGGTGGCGGGTCAGCt                                                                                <  1:3030526/93‑1 (MQ=255)

         gACGTTAGGAGTTCAGTTCGCGGATCAGGAACGCCTGTTTGTCGGCCTCTGACCAGCTTTCGTAGTCGCCGATACCGAGGTGGCGGGTCAGCt                                                                                >  1:5555093/1‑93 (MQ=255)

            gTTTGGAGTTCAGTTCGCGGATCAGGAACGCCTGTTTGTCGGCCTCTGACCAGCTTTCGTAGTCGCCGATACCGAGGTGGCGGGTCAGCTCGc                                                                             >  1:2375829/1‑93 (MQ=255)

             tttGGAGTTCAGTTCGCGGATCAGGAACGCCTGTTTGTCGGCCTCTGACCAGCTTTCGTAGTCGCCGATACCGAGGTGGCGGGTCAGCTCGcc                                                                            >  2:1932908/1‑93 (MQ=255)

                ggAGTTCAGTTCGCGGATCAGGAACGCCTGTTTGTCGGCCTCTGACCAGCTTTCGTAGTCGCCGATACCGAGGTGGCGGGTCAGCTCGCCCAg                                                                         >  1:4699786/1‑93 (MQ=255)

                  agttcagttcGCGGATCAGGAACGCCTGTTTATCGGCCTCTGACCAGCTTTCGTAGGCGCCGATACCGAGGTGGAGGGGCACCCCGcccctcg                                                                       >  1:192324/1‑89 (MQ=255)

                   gttcagttcGCGGATCAGGAACGCCTGTTTGTCGGCCTCTGACCAGCTTTCGTAGTCGCCGATACCGAGGTGGCGGGTCAGCTCGCCCAgcgc                                                                      <  1:5899903/93‑1 (MQ=255)

                     tcagttcGCGGATCAGGAACGCCTGTTTGTCGGCCTCTGACCAGCTTTCGTAGTCGCCGATACCGAGGTGGCGGGTCAGCTCGCCCAGCGCtt                                                                    <  1:1344143/93‑1 (MQ=255)

                      cagttcGCGGATCAGGAACGCCTGTTTGTCGGCCTCTGACCAGCTTTCGTAGTCGCCGATACCGAGGTGGCGGGTCAGCTCGCCCAGCGCTTc                                                                   <  1:6479886/93‑1 (MQ=255)

                      cagttcGCGGATCAGGAACGCCTGTTTGTCGGCCTCTGACCAGCTTTCGTAGTCGCCGATACCGAGGTGGCGGGTCAGCTCGCCCAGCGCTTc                                                                   <  2:477588/93‑1 (MQ=255)

                      cagttcGCGGATCAGGAACGCCTGTTTGTCGGCCTCTGACCAGCTTTCGTAGTCGCCGATACCGAGGTGGCGGGTCAGCTCGCCCAGCGCTTc                                                                   <  2:1231529/93‑1 (MQ=255)

                        gttcGCGGATCAGGAACGCCTGTTTGTCGGCCTCTGACCAGCTTTCGTAGTCGCCGATACCGAGGTGGCGGGTCAGCTCGCCCAGCGCTTCgg                                                                 >  2:3817056/1‑93 (MQ=255)

                          tcGCGGATCAGGAACGCCTGTTTGTCGGCCTCTGACCAGCTTTCGTAGTCGCCGATACCGAGGTGGCGGGTCAGCTCGCCCAGCGCTTCGGTa                                                               <  2:460145/93‑1 (MQ=255)

                             cgGATCAGGAACGCCTGTTTGTCGGCCTCTGACCAGCTTTCGTAGTCGCCGATACCGAGGTGGCGGGTCAGCTCGCCCAGCGCTTCGGTATGa                                                            <  2:3139273/93‑1 (MQ=255)

                             cgGATCAGGAACGCCTGTTTGTCGGCCTCTGACCAGCTTTCGTAGTCGCCGATACCGAGGTGGCGGGTCAGCTCGCCCAGCGCTTCGGTATGa                                                            <  2:6517077/93‑1 (MQ=255)

                              ggATCAGGAACGCCTGTTTGTCGGCCTCTGACCAGCTTTCGTAGTCGCCGATACCGAGGTGGCGGGTCAGCTCGCCCAGCGCTTCGGTATGAc                                                           >  2:1814603/1‑93 (MQ=255)

                               gATCAGGAACGCCTGTTTGTCGGCCTCTGACCAGCTTTCGTAGTCGCCGATACCGAGGTGGCGGGTCAGCTCGCCCAGCGCTTCGGTATGAcg                                                          >  1:4349877/1‑93 (MQ=255)

                                aTCAGGAACGCCTGTTTGTCGGCCTCTGACCAGCTTTCGTAGTCGCCGATACCGAGGTGGCGGGTCAGCTCGCCCAGCGCTTCGGTATGAcgc                                                         >  2:340182/1‑93 (MQ=255)

                                aTCAGGAACGCCTGTTTGTCGGCCTCTGACCAGCTTTCGTAGTCGCCGATACCGAGGTGGCGGGTCAGCTCGCCCAGCGCTTCGGTATGAcgc                                                         >  1:4050237/1‑93 (MQ=255)

                                aTCAGGAACGCCTGTTTGTCGGCCTCTGACCAGCTTTCGTAGTCGCCGATACCGAGGTGGCGGGTCAGCTCGCCCAGCGCTTCGGTATGAcgc                                                         <  2:3543867/93‑1 (MQ=255)

                                aTCAGGAACGCCTGTTTGTCGGCCTCTGACCAGCTTTCGTAGTCGCCGATACCGAGGTGGCGGGTCAGCTCGCCCAGCGCTTCGGTATGAcgc                                                         >  1:4802872/1‑93 (MQ=255)

                                aTCAGGAACGCCTGTTTGTCGGCCTCTGACCAGCTTTCGTAGTCGCCGATACCGAGGTGGCGGGTCAGCTCGCCCAGCGCTTCGGTATGAcgc                                                         <  1:9687/93‑1 (MQ=255)

                                 tCAGGAACGCCTGTTTGTCGGCCTCTGACCAGCTTTCGTAGTCGCCGATACCGAGGTGGCGGGTCAGCTCGCCCAGCGCTTCGGTATGAcgcg                                                        >  2:5839626/1‑93 (MQ=255)

                                 tCAGGAACGCCTGTTTGTCGGCCTCTGACCAGCTTTCGTAGTCGCCGATACCGAGGTGGCGGGTCAGCTCGCCCAGCGCTTCGGTATGAcgcg                                                        >  2:2523348/1‑93 (MQ=255)

                                    ggAACGCCTGTTTGTCGGCCTCTGACCAGCTTTCGTAGTCGCCGATACCGAGGTGGCGGGTCAGCTCGCCCAGCGCTTCGGTATGACGCGTGc                                                     >  1:3234866/1‑93 (MQ=255)

                                    ggAACGCCTGTTTGTCGGCCTCTGACCAGCTTTCGTAGTCGCCGATACCGAGGTGGCGGGTCAGCTCGCCCAGCGCTTCGGTATGACGCGTGc                                                     >  2:5950083/1‑93 (MQ=255)

                                    ggAACGCCTGTTTGTCGGCCTCTGACCAGCTTTCGTAGTCGCCGATACCGAGGTGGCGGGTCAGCTCGCCCAGCGCTTCGGTATGACGCGTGc                                                     >  2:4047873/1‑93 (MQ=255)

                                      aaCGCCTGTTTGTCGGCCTCTGACCAGCTTTCGTAGTCGCCGATACCGAGGTGGCGGGTCAGCTCGCCCAGCGCTTCGGTATGACGCGTGctc                                                   <  2:4335005/93‑1 (MQ=255)

                                       aCGCGTGTTTGTCGTCCTCTGACCAGCTTTCGTAGTCGCCGATACCGAGGTGGCGGGTCAGCTCGCCCAGCGCTTCGGTATGACGCGTGctct                                                  <  1:5691452/93‑1 (MQ=255)

                                          ccTGTTTGTCGGCCTCTGACCAGCTTTCGTAGTCGCCGATACCGAGGTGGCGGGTCAGCTCGCCCAGCGCTTCGGTATGACGCGTGCTCTCCt                                               <  1:6667055/93‑1 (MQ=255)

                                          ccTGTTTGTCGGCCTCTGACCAGCTTTCGTAGTCGCCGATACCGAGGTGGCGGGTCAGCTCGCCCAGCGCTTCGGTATGACGCGTGCTCTCCt                                               >  2:1683045/1‑93 (MQ=255)

                                          ccTGTTTGTCGGCCTCTGACCAGCTTTCGTAGTCGCCGATACCGAGGTGGCGGGTCAGCTCGCCCAGCGCTTCGGTATGACGCGTGCTCTCCt                                               <  2:6707928/93‑1 (MQ=255)

                                          ccTGTTTGTCGGCCTCTGACCAGCTTTCGTAGTCGCCGATACCGAGGTGGCGGGTCAGCTCGCCCAGCGCTTCGGTATGACGCGTGCTCTCCt                                               <  1:5105839/93‑1 (MQ=255)

                                           cTGTTTGTCGGTCTCTGACCAGCTTTCGTAGTCGCCGATACCGAGGTGGCGGGTCAGCTCGCCCAGCGCTTCGGTATGACGCGTGCTCTCCTg                                              >  2:5245270/1‑93 (MQ=255)

                                           cTGTTTGTCGGCCTCTGACCAGCTTTCGTAGTCGCCGATACCGAGGTGGCGGGTCAGCTCGCCCAGCGCTTCGGTATGACGCGTGCTCTCCTg                                              >  1:2490931/1‑93 (MQ=255)

                                           cTGTTTGTCGGCCTCTGACCAGCTTTCGTAGTCGCCGATACCGAGGTGGCGGGTCAGCTCGCCCAGCGCTTCGGTATGACGCGTGCTCTCCTg                                              >  1:1915556/1‑93 (MQ=255)

                                            tGTTTGTCGGCCTCTGACCAGCTTTCGTAGTCGCCGATACCGAGGTGGCGGGTCAGCTCGCCCAGCGCTTCGGTATGACGCGTGCTCTCCTGa                                             <  2:4578155/93‑1 (MQ=255)

                                             gTTTGTCGGCCTCTGACCAGCTTTCGTAGTCGCCGATACCGAGGTGGCGGGTCAGCTCGCCCAGCGCTTCGGTATGACGCGTGCTCTCCTGAc                                            >  1:5205192/1‑93 (MQ=255)

                                             gTTTGTCGGCCTCTGACCAGCTTTCGTAGTCGCCGATACCGAGGTGGCGGGTCAGCTCGCCCAGCGCTTCGGTATGACGCGTGCTCTCCTGAc                                            >  2:3054764/1‑93 (MQ=255)

                                             gTTTGTCGGCCTCTGACCAGCTTTCGAAGTCGCCGATACCGAGGTGGCGAGTCAGCTCGCCCAGCGCTTCGGTATGACGCGTGCTCTCCTGAc                                            <  2:1489328/93‑1 (MQ=255)

                                                tGTCGGCCTCTGACCAGCTTTCGTAGTCGCCGATACCGAGGTGGCGGGTCAGCTCGCCCAGCGCTTCGGTATGACGCGTGCTCTCCTGACGGa                                         <  1:226119/93‑1 (MQ=255)

                                                  tCGGCCTCTGACCAGCTTTCGTAGTCGCCGATACCGAGGTGGCGGGTCAGCTCGCCCAGCGCTTCGGTATGACGCGTGCTCTCCTGACGGata                                       >  2:435463/1‑93 (MQ=255)

                                                  gcGGCCTCTGACCAGCTTTCGTAGTCGCCGATACCGAGGTGGCGGGTCAGCTCGCCCAGCGCTGCGGTATGACGCGTGCTCTCCTGACGGata                                       <  1:3079697/92‑1 (MQ=255)

                                                    ggCCTCTGACCAGCTTTCGTAGTCGCCGATACCGAGGTGGCGGGTCAGCTCGCCCAGCGCTTCGGTATGACGCGTGCTCTCCTGACGGATATc                                     <  1:6388441/93‑1 (MQ=255)

                                                     gCCTCTGACCAGCTTTCGTAGTCGCCGATACCGAGGTGGCGGGTCAGCTCGCCCAGCGCTTCGGTATGACGCGTGCTCTCCTGACGGATATCa                                    >  2:3976663/1‑93 (MQ=255)

                                                      ccTCTGACCAGCTTTCGTAGTCGCCGATACCGAGGTGGCGGGTCAGCTCGCCCAGCGCTTCGGTATGACGCGTGCTCTCCTGACGGATATCaa                                   >  1:2085915/1‑93 (MQ=255)

                                                       ctctGACCAGCTTTCGTAGTCGCCGATACCGAGGTGGCGGGTCAGCTCGCCCAGCGCTTCGGTATGACGCGTGCTCTCCTGACGGATATCAAt                                  >  1:4542470/1‑93 (MQ=255)

                                                       ctctGACCAGCTTTCGTAGTCGCCGATACCGAGGTGGCGGGTCAGCTCGCCCAGCGCTTCGGTATGACGCGTGCTCTCCTGACGGATATCAAt                                  >  1:884458/1‑93 (MQ=255)

                                                        tctGACCAGCTTTCGTAGTCGCCGATACCGAGGTGGCGGGTCAGCTCGCCCAGCGCTTCGGTATGACGCGTGCTCTCCTGACGGATATCAATa                                 >  1:5986395/1‑93 (MQ=255)

                                                        tctGACCAGCTTTCGTAGTCGCCGATACCGAGGTGGCGGGTCAGCTCGCCCAGCGCTTCGGTATGACGCGTGCTCTCCTGACGGATATCAATa                                 <  1:1665809/93‑1 (MQ=255)

                                                        tctGACCAGCTTTCGTAGTCGCCGATACCGAGGTGGCGGGTCAGCTCGCCCAGCGCTTCGGTATGACGCGTGCTCTCCTGACGGATATCAATa                                 >  2:2065364/1‑93 (MQ=255)

                                                          tGACCAGCTTTCGTAGTCGCCGATACCGAGGTGGCGGGTCAGCTCGCCCAGCGCTTCGGTATGACGCGTGCTCTCCTGACGGATATCAATACg                               <  2:2462906/93‑1 (MQ=255)

                                                            aCCAGCTTTCGTAGTCGCCGATACCGAGGTGGCGGGTCAGCTCGCCCAGCGCTTCGGTATGACGCGTGCTCTCCTGACGGATATCAATACGGa                             >  1:6303294/1‑93 (MQ=255)

                                                            aCCAGCTTTCGTAGTCGCCGATACCGAGGTGGCGGGTCAGCTCGCCCAGCGCTTCGGTATGACGCGTGCTCTCCTGACGGATATCAATACGGa                             <  1:2577616/93‑1 (MQ=255)

                                                            aCCAGCTTTCGTAGTCGCCGATACCGAGGTGGCGGGTCAGCTCGCCCAGCGCTTCGGTATGACGCGTGCTCTCCTGACGGATATCAATACGGa                             <  1:6008191/93‑1 (MQ=255)

                                                               aGCTTTCGTAGTCGCCGATACCGAGGTGGCGGGTCAGCTCGCCCAGCGCTTCGGTATGACGCGTGCTCTCCTGACGGATATCAATACGGACCa                          <  1:1469473/93‑1 (MQ=255)

                                                                gCTTTCGTAGTCGCCGATACCGAGGTGGCGGGTCAGCTCGCCCAGCGCTTCGGTATGACGCGTGCTCTCCTGACGGATATCAATACGGACCAg                         >  1:2789114/1‑93 (MQ=255)

                                                                 cTTTCGTAGTCGCCGATACCGAGGTGGCGGGTCAGCTCGCCCAGCGCTTCGGTATGACGCGTGCTCTCCTGACGGATATCAATACGGACCAGc                        <  1:3373094/93‑1 (MQ=255)

                                                                 cTTTCGTAGTCGCCGATACCGAGGTGGCGGGTCAGCTCGCCCAGCGCTTCGGTATGACGCGTGCTCTCCTGACGGATATCAATACGGACCAGc                        <  1:4214919/93‑1 (MQ=255)

                                                                   ttCGTAGTCGCCGATACCGAGGTGGCGGGTCAGCTCGCCCAGCGCTTCGGTATGACGCGTGCTCTCCTGACGGGTATCAATACGCACCAGCgg                      <  1:6554586/93‑1 (MQ=255)

                                                                   ttCGTAGTCGCCGATACCGAGGTGGCGGGTCAGCTCGCCCAGCGCTTCGGTATGACGCGTGCTCTCCTGACGGATATCAATACGGACCAGCgg                      <  1:220566/93‑1 (MQ=255)

                                                                   ttCGTAGTCGCCGATACCGAGGTGGCGGGTCAGCTCGCCCAGCGCTTCGGTATGACGCGTGCTCTCCTGACGGATATCAATACGGACCAGCgg                      <  2:4166950/93‑1 (MQ=255)

                                                                   atcttAGTCGCCGATACCGAGGTGGCGGGGCAGCTCGCCCAGCGCTTCGGTATGACGCGTGCTCTCCTGACGTATATCAATACGGTCCAGCgg                      <  1:4468238/89‑1 (MQ=255)

                                                                     cGTAGTCGCCGATACCGAGGTGGCGGGTCAGCTCGCCCAGCGCTTCGGTATGACGCGTGCTCTCCTGACGGATATCAATACGGACCAGCGGTa                    <  2:77734/93‑1 (MQ=255)

                                                                     cGTAGTCGCCGATACCGAGGTGGCGGGTCAGCTCGCCCAGCGCTTCGGTATGACGCGTGCTCTCCTGACGGATATCAATACGGACCAGCGGTa                    <  1:3406955/93‑1 (MQ=255)

                                                                      gTAGTCGCCGATACCGAGGTGGCGGGTCAGCTCGCCCAGCGCTTCGGTATGACGCGTGCTCTCCTGACGGATATCAATACGGACCAGCGGTAc                   <  1:6211051/93‑1 (MQ=255)

                                                                       tAGTCGCCGATACCGAGGTGGCGGGTCAGCTCGCCCAGCGCTTCGGTATGACGCGTGCTCTCCTGACGGATATCAATACGGACCAGCGGTACg                  >  2:6071659/1‑93 (MQ=255)

                                                                        aGTCGCCGATACCGAGGTGGCGGGTCAGCTCGCCCAGCGCTTCGGTATGACGCGTGCTCTCCTGACGGATATCAATACGGACCAGCGGTACGc                 >  1:1094212/1‑93 (MQ=255)

                                                                         gTCGCCGATACCGAGGTGGCGGGTCAGCTCGCCCAGCGCTTCGGTATGACGCGTGCTCTCCTGACGGATATCAATACGGACCAGCGGTACGcc                >  1:3093714/1‑93 (MQ=255)

                                                                         gTCGCCGATACCGAGGTGGCGGGTCAGCTCGCCCAGCGCTTCGGTATGACGCGTGCTCTCCTGACGGATATCAATACGGACCAGCGGTACGcc                >  2:5682626/1‑93 (MQ=255)

                                                                           cGCCGATACCGAGGTGGCGGGTCAGCTCGCCCAGCGCTTCGGTATGACGCGTGCTCTCCTGACGGATATCAATACGGACCAGCGGTACGCCGa              >  2:6415261/1‑93 (MQ=255)

                                                                              cGATACCGAGGTGGCGGGTCAGCTCGCCCAGCGCTTCGGTATGACGCGTGCTCTCCTGACGGATATCAATACGGACCAGCGGTACGCCGAAAc           <  1:2781028/93‑1 (MQ=255)

                                                                               gATACCGAGGTGGCGGGTCAGCTCGCCCAGCGCTTCGGTATGACGCGTGCTCTCCTGACGGATATCAATACGGACCAGCGGTACGCCGAAACa          >  1:1579735/1‑93 (MQ=255)

                                                                                aTACCGAGGTGGCGGGTCAGCTCGCCCAGCGCTTCGGTATGACGCGTGCTCTCCTGACGGATATCAATACGGACCAGCGGTACGCCGAAACAt         >  1:743432/1‑93 (MQ=255)

                                                                                aTACCGAGGTGGCGGGTCAGCTCGCCCAGCGCTTCGGTATGACGCGTGCTCTCCTGACGGATATCAATACGGACCAGCGGTACGCCGAAACAt         >  1:4621600/1‑93 (MQ=255)

                                                                                aTACCGAGGTGGCGGGTCAGCTCGCCCAGCGCTTCGGTATGACGCGTGCTCTCCTGACGGATATCAATACGGACCAGCGGTACGCCGAAACAt         <  2:3973274/93‑1 (MQ=255)

                                                                                aTACCGAGGTGGCGGGGCAGCTCGCCCAGCGCTTCGGTATGACGCGTGCTCTCCTGACGGATATCAATACGGACCAGCGGGACGCCGAAACAt         >  1:3915012/1‑93 (MQ=255)

                                                                                 tACCGAGGTGGCGGGTCAGCTCGCCCAGCGCTTCGGTATGACGCGTGCTCTCCTGACGGATATCAATACGGACCAGCGGTACGCCGAAACAtt        <  2:5705865/93‑1 (MQ=255)

                                                                                 tACCGAGGTGGCGGGTCAGCTCGCCCAGCGCTTCGGTATGACGCGTGCTCTCCTGACGGATATCAATACGGACCAGCGGTACGCCGAAACAtt        <  1:1236151/93‑1 (MQ=255)

                                                                                 tACCGAGGGGGCGGGTCAGCTCGCCCAGCGCTTCGGGATGACGCGTGCTCTCCTGACGGGTATGAATACGGACCAGCGGTACCCCGAAACAtt        >  2:4948632/1‑93 (MQ=255)

                                                                                   ccGAGGTGGCGGGTCAGCTCGCCCAGCGCTTCGGTATGACGCGTGCTCTCCTGACGGATATCAATACGGACCAGCGGTACGCCGAAACATTTc      <  1:3699647/93‑1 (MQ=255)

                                                                                   ccGAGGTGGCGGGTCAGCTCGCCCAGCGCTTCGGTATGACGCGTGCTCTCCTGACGGATATCAATACGGACCAGCGGTACGCCGAAACATTTc      >  2:1124225/1‑93 (MQ=255)

                                                                                     gAGGTGGCGGGTCAGCTCGGCCAGCGCTTCGGTATGACGCGTGCTCTCCTGACGGATCTCAATACGGACCAGCGGTACCCCGAAACCTTTAAc    >  1:4149907/1‑93 (MQ=255)

                                                                                     gAGGTGGCGGGTCAGCTCGCCCAGCGCTTCGGTATGACGCGTGCTCTCCTGACGGATATCAATACGGACCAGCGGTACGCCGAAACATTTCAc    <  1:5487829/93‑1 (MQ=255)

                                                                                      aGGTGGCGGGTCAGCTCGCCCAGCGCTTCGGTATGACGCGTGCTCTCCTGACGGATATCAATACGGACCAGCGGTACGCCGAAACATTTCAcg   <  2:4932183/93‑1 (MQ=255)

                                                                                      aGGTGGCGGGTCAGCTCGCCCAGCGCTTCGGTATGACGCGTGCTCTCCTGACGGATATCAATACGGACCAGCGGTACGCCGAAACATTTCAcg   >  2:1091950/1‑93 (MQ=255)

                                                                                      aGGTGGCGGGTCAGCTCGCCCAGCGCTTCGGTATGACGCGTGCTCTCCTGACGGATATCAATACGGACCAGCGGTACGCCGAAACATTTCAcg   <  2:3483562/93‑1 (MQ=255)

GCAGAAGCGGACGTTTGGAGTTCAGTTCGCGGATCAGGAACGCCTGTTTGTCGGCCTCTGACCAGCTTTCGTAGTCGCCGATACCGAGGTAGCGGGTCAGCTCGCCCAGCGCTTCGGTATGACGCGTGCTCTCCTGACGGATATCAATACGGACCAGCGGTACGCCGAAACATTTCACGC  >  NC_000913/4149354‑4149533

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 17 ≤` `ATCG/ATCG` `< 26 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`