BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,198,118	A→G	D87G (GAC→GGC)	hupA →	DNA‑binding protein HU‑alpha

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,198,118	0	A	G	100.0%	1094.8 / NA	334	D87G (GAC→GGC)	hupA	DNA‑binding protein HU‑alpha
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (146/188); total (146/188)

Only 100 of 340 total aligned reads displayed.

AGCGTACTGGCCGCAACCCGCAGACCGGTAAAGAAATCAAAATTGCCGCAGCTAACGTACCGGCATTTGTTTCTGGCAAGGCACTGAAAGACGCAGTTAAGTAAGATTGCGTGGCAGTGAACAGTTTTAACGAAGGGGTGGTTTCACCCCTTTTGTCTTTCTGGCGTCGATCATTGATGC  >  NC_000913/4198028‑4198207

aGCGTACTGGCCGCAACCCGCAGACCGGTAAAGAAATCAAAATTGCCGCAGCTAACGTACCGGCATTTGTTTCTGGCAAGGCACTGAAAGGCg                                                                                         <  1:5758607/93‑1 (MQ=255)

   gTACTGGCCGCAACCCGCAGACCGGTAAAGAAATCAAAATTGCCGCAGCTAACGTACCGGCATTTGTTTCTGGCAAGGCACTGAAAGGCGCAg                                                                                      >  1:10253/1‑93 (MQ=255)

    tACTGGCCGCAACCCGCAGACCGGTAAAGAAATCAAAATTGCCGCAGCTAACGTACCGGCATTTGTTTCTGGCAAGGCACTGAAAGGCGCAGt                                                                                     >  1:3994078/1‑93 (MQ=255)

    tACTGGCCGCAACCCGCAGACCGGTAAAGAAATCAAAATTGCCGCAGCTAACGTACCGGCATTTGTTTCTGGCAAGGCACTGAAAGGCGCAGt                                                                                     >  1:4496588/1‑93 (MQ=255)

    tACTGGCCGCAACCCGCAGACCGGTAAAGAAATCAAAATTGCCGCAGCTAACGTACCGGCATTTGTTTCTGGCAAGGCACTGAAAGGCGCAGt                                                                                     >  1:5383044/1‑93 (MQ=255)

    tACTGGCCGCAACCCGCAGACCGGTAAAGAAATCAAAATTGCCGCAGCTAACGTACCGGCATTTGTTTCTGGCAAGGCACTGAAAGGCGCAGt                                                                                     <  2:3871972/93‑1 (MQ=255)

      cTGGCCGCAACCCGCAGACCGGTAAAGAAATCAAAATTGCCGCAGCTAACGTACCGGCATTTGTTTCTGGCAAGGCACTGAAAGGCGCAGTta                                                                                   >  1:2670571/1‑93 (MQ=255)

       tGGCCGCAACCCGCAGACCGGTAAAGAAATCAAAATTGCCGCAGCTAACGTACCGGCATTTGTTTCTGGCAAGGCACTGAAAGGCGCAGTtaa                                                                                  <  1:1476171/93‑1 (MQ=255)

       tGGCCGCAACCCGCAGACCGGTAAAGAAATCAAAATTGCCGCAGCTAACGTACCGGCATTTGTTTCTGGCAAGGCACTGAAAGGCGCAGTtaa                                                                                  <  2:1953830/93‑1 (MQ=255)

           cGCAACCCGCAGACCGGTAAAGAAATCAAAATTGCCGCAGCTAACGTACCGGCATTTGTTTCTGGCAAGGCACTGAAAGGCGCAGTtaagtaa                                                                              <  2:6588802/93‑1 (MQ=255)

             cAACCCGCAGACCGGTAAAGAAATCAAAATTGCCGCAGCTAACGTACCGGCATTTGTTTCTGGCAAGGCACTGAAAGGCGCAGTTAAGTAAGa                                                                            <  1:5021905/93‑1 (MQ=255)

              aaCCCGCAGACCGGTAAAGAAATCAAAATTGCCGCAGCTAACGTACCGGCATTTGTTTCTGGCAAGGCACTGAAAGGCGCAGTTAAGTAAGAt                                                                           <  1:3796035/93‑1 (MQ=255)

              aaCCCGCAGACCGGTAAAGAAATCAAAATTGCCGCAGCTAACGTACCGGCATTTGTTTCTGGCAAGGCACTGAAAGGCGCAGTTAAGTAAGAt                                                                           <  2:5482785/93‑1 (MQ=255)

               aCCCGCAGACCGGTAAAGAAATCAAAATTGCCGCAGCTAACGTACCGGCATTTGTTTCTGGCAAGGCACTGAAAGGCGCAGTTAAGTAAGAtt                                                                          <  1:6188521/93‑1 (MQ=255)

               aCCCGCAGACCGGTAAAGAAATCAAAATTGCCGCAGCTAACGTACCGGCATTTGTTTCTGGCAAGGCACTGAAAGGCGCAGTTAAGTAAGAtt                                                                          >  2:5961564/1‑93 (MQ=255)

                cccGCAGACCGGTAAAGAAATCAAAATTGCCGCAGCTAACGTACCGGCATTTGTTTCTGGCAAGGCACTGAAAGGCGCAGTTAAGTAAGATTg                                                                         <  2:4247808/93‑1 (MQ=255)

                cccGCAGACCGGTAAAGAAATCAAAATTGCCGCAGCTAACGTACCGGCATTTGTTTCTGGCAAGGCACTGAAAGGCGCAGTTAAGTAAGATTg                                                                         <  1:4809071/93‑1 (MQ=255)

                  cGCAGACCGGTAAAGAAATCAAAATTGCCGCAGCTAACGTACCGGCATTTGTTTCTGGCAAGGCACTGAAAGGCGCAGTTAAGTAAGATTGCg                                                                       <  2:1804657/93‑1 (MQ=255)

                   gCAGACCGGTAAAGAAATCAAAATTGCCGCAGCTAACGTACCGGCATTTGTTTCTGGCAAGGCACTGAAAGGCGCAGTTAAGTAAGATTGCGt                                                                      <  2:407871/93‑1 (MQ=255)

                   gCAGACCGGTAAAGAAATCAAAATTGCCGCAGCTAACGTACCGGCATTTGTTTCTGGCAAGGCACTGAAAGGCGCAGTTAAGTAAGATTGCGt                                                                      <  2:814278/93‑1 (MQ=255)

                    cAGACCGGTAAAGAAATCAAAATTGCCGCAGCTAACGTACCGGCATTTGTTTCTGGCAAGGCACTGAAAGGCGCAGTTAAGTAAGATTGCGTg                                                                     <  2:5896426/93‑1 (MQ=255)

                     aGACCGGTAAAGAAATCAAAATTGCCGCAGCTAACGTACCGGCATTTGTTTCTGGCAAGGCACTGAAAGGCGCAGTTAAGTAAGATTGCGTgg                                                                    >  2:3764463/1‑93 (MQ=255)

                     aGACCGGTAAAGAAATCAAAATTGCCGCAGCTAACGTACCGGCATTTGTTTCTGGCAAGGCACTGAAAGGCGCAGTTAAGTAAGATTGCGTgg                                                                    >  1:668921/1‑93 (MQ=255)

                      gACCGGTAAAGAAATCAAAATTGCCGCAGCTAACGTACCGGCATTTGTTTCTGGCAAGGCACTGAAAGNCGCAGTTAAGTAAGATTGCGTGGc                                                                   >  1:334998/1‑93 (MQ=255)

                      gACCGGTAAAGAAATCAAAATTGCCGCAGCTAACGTACCGGCATTTGTTTCTGGCAAGGCACTGAAAGGCGCAGTTAAGTAAGATTGCGTGGc                                                                   <  2:116650/93‑1 (MQ=255)

                      gACCGGTAAAGAAATCAAAATTGCCGCAGCTAACGTACCGGCATTTGTTTCTGGCAAGGCACTGAAAGGCGCAGTTAAGTAAGATTGCGTGGc                                                                   >  2:883202/1‑93 (MQ=255)

                       aCCGGTAAAGAAATCAAAATTGCCGCAGCTAACGTACCGGCATTTGTTTCTGGCAAGGCACTGAAAGGCGCAGTTAAGTAAGATTGCGTGGCa                                                                  >  1:2125760/1‑93 (MQ=255)

                          ggTAAAGAAATCAAAATTGCCGCAGCTAACGTACCGGCATTTGTTTCTGGCAAGGCACTGAAAGGCGCAGTTAAGTAAGATTGCGTGGCAGTg                                                               >  1:1950884/1‑93 (MQ=255)

                           gTAAAGAAATCAAAATTGCCGCAGCTAACGTACCGGCATTTGTTTCTGGCAAGGCACTGAAAGGCGCCGTTAAGTAAGATTGCGTGGCCGTGa                                                              >  1:3254164/1‑93 (MQ=255)

                             aaaGAAATCAAAATTGCCGCAGCTAACGTACCGGCATTTGTTTCTGGCAAGGCACTGAAAGGCGCAGTTAAGTAAGATTGCGTGGCAGTGAAc                                                            >  1:3561223/1‑93 (MQ=255)

                              aaGAAATCAAAATTGCCGCAGCTAACGTACCGGCATTTGTTTCTGGCAAGGCACTGAAAGGCGCAGTTAAGTAAGATTGCGTGGCAGTGAACa                                                           <  1:241695/93‑1 (MQ=255)

                              aaGAAATCAAAATTGCCGCAGCTAACGTACCGGCATTTGTTTCTGGCAAGGCACTGAAAGGCGCAGTTAAGTAAGATTGCGTGGCAGTGAACa                                                           >  1:896943/1‑93 (MQ=255)

                               aGAAATCAAAATTGCCGCAGCTAACGTACCGGCATTTGTTTCTGGCAAGGCACTGAAAGGCGCAGTTAAGTAAGATTGCGTGGCAGTGAACAg                                                          >  2:2312846/1‑93 (MQ=255)

                                gAAATCAAAATTGCCGCAGCTAACGTACCGGCATTTGTTTCTGGCAAGGCACTGAAAGGCGCAGTTAAGTAAGATTGCGTGGCAGTGAACAGt                                                         >  1:3638821/1‑93 (MQ=255)

                                 aaaTCAAAATTGCCGCAGCTAACGTACCGGCATTTGTTTCTGGCAAGGCACTGAAAGGCGCAGTTAAGTAAGATTGCGTGGCAGTGAACAGtt                                                        <  2:3650545/93‑1 (MQ=255)

                                  aaTCAAAATTGCCGCAGCTAACGTACCGGCATTTGTTTCTGGCAAGGCACTGAAAGGCGCAGTTAAGTAAGATTGCGTGGCAGTGAACAGttt                                                       >  2:706037/1‑93 (MQ=255)

                                  aaTCAAAATTGCCGCAGCTAACGTACCGGCATTTGTTTCTGGCAAGGCACTGAAAGGCGCAGTTAAGTAAGATTGCGTGGCAGTGAACAGttt                                                       >  1:1711735/1‑93 (MQ=255)

                                  aaTCAAAATTGCCGCAGCTAACGTACCGGCATTTGTTTCTGGCAAGGCACTGAAAGGCGCAGTTAAGTAAGATTGCGTGGCAGTGAACAGttt                                                       >  1:787809/1‑93 (MQ=255)

                                   aTCAAAATTGCCGCAGCTAACGTACCGGCATTTGTTTCTGGCAAGGCACTGAAAGGCGCAGTTAAGTAAGATTGCGTGGCAGTGAACAGtttt                                                      <  2:3472630/93‑1 (MQ=255)

                                   aTCAAAATTGCCGCAGCTAACGTACCGGCATTTGTTTCTGGCAAGGCACTGAAAGGCGCAGTTAAGTAAGATTGCGTGGCAGTGAACAGtttt                                                      <  2:4550312/93‑1 (MQ=255)

                                    tCAAAATTGCCGCAGCTAACGTACCGGCATTTGTTTCTGGCAAGGCACTGAAAGGCGCAGTTAAGTAAGATTGCGTGGCAGTGAACAGTTTTa                                                     >  2:256925/1‑93 (MQ=255)

                                    tCAAAATTGCCGCAGCTAACGTACCGGCATTTGTTTCTGGCAAGGCACTGAAAGGCGCAGTTAAGTAAGATTGCGTGGCAGTGAACAGTTTTa                                                     >  2:3629282/1‑93 (MQ=255)

                                    acAAAATTGCCGCAGCTAACGTACCGGCATTTGTTTCTGGCAAGGCACTGAAAGGCGCAGTTAAGTAAGATTGCGTGGCAGTGAACAGTTTTa                                                     <  2:1883919/92‑1 (MQ=255)

                                      aaaaTTGCCGCAGCTAACGTACCGGCATTTGTTTCTGGCAAGGCACTGAAAGGCGCAGTTAAGTAAGATTGCGTGGCAGTGAACAGTTTTAAc                                                   >  2:2975871/1‑93 (MQ=255)

                                      aaaaTTGCCGCAGCTAACGTACCGGCATTTGTTTCTGGCAAGGCACTGAAAGGCGCAGTTAAGTAAGATTGCGTGGCAGTGAACAGTTTTAAc                                                   <  1:5920471/93‑1 (MQ=255)

                                        aaTTGCCGCAGCTAACGTACCGGCATTTGTTTCTGGCAAGGCACTGAAAGGCGCAGTTAAGTAAGATTGCGTGGCAGTGAACAGTTTTAACGa                                                 >  2:6182084/1‑93 (MQ=255)

                                        aaTTGCCGCAGCTAACGTACCGGCATTTGTTTCTGGCAAGGCACTGAAAGGCGCAGTTAAGTAAGATTGCGTGGCAGTGAACAGTTTTAACGa                                                 >  1:4137083/1‑93 (MQ=255)

                                          ttGCCGCAGCTAACGTACCGGCATTTGTTTCTGGCAAGGCACTGAAAGGCGCAGTTAAGTAAGATTGCGTGGCAGTGAACAGTTTTAACGAAg                                               >  1:4653248/1‑93 (MQ=255)

                                           tGCCGCAGCTAACGTACCGGCATTTGTTTCTGGCAAGGCACTGAAAGGCGCAGTTAAGTAAGATTGCGTGGCAGTGAACAGTTTTAACGAAgg                                              <  1:5810778/93‑1 (MQ=255)

                                           tGCCGCAGCTAACGTACCGGCATTTGTTTCTGGCAAGGCACTGAAAGGCGCAGTTAAGTAAGATCGCGCGCCAGTGAACAGTTTTAACGAAgg                                              >  1:1613665/1‑93 (MQ=255)

                                            gCCGCAGCTAACGTACCGGCATTTGTTTCTGGCAAGGCACTGAAAGGCGCAGTTAAGTAAGATTGCGTGGCAGTGAACAGTTTTAACGAAggg                                             <  1:2773653/93‑1 (MQ=255)

                                            gCCGCAGCTAACGTACCGGCATTTGTTTCTGGCAAGGCACTGAAAGGCGCAGTTAAGTAAGATTGCGTGGCAGTGAACAGTTTTAACGAAggg                                             <  2:1505153/93‑1 (MQ=255)

                                             ccGCAGCTAACGTACCGGCATTTGTTTCTGGCAAGGCACTGAAAGGCGCAGTTAAGTAAGATTGCGTGGCAGTGAACAGTTTTAACGAAgggg                                            <  1:2737746/93‑1 (MQ=255)

                                             ccGCAGCTAACGTACCGGCATTTGTTTCTGGCAAGGCACTGAAAGGCGCAGTTAAGTAAGATTGCGTGGCAGTGAACAGTTTTAACGAAgggg                                            <  2:3329740/93‑1 (MQ=255)

                                              cGCAGCTAACGTACCGGCATTTGTTTCTGGCAAGGCACTGAAAGGCGCAGTTAAGTAAGATTGCGTGGCAGTGAACAGTTTTAACGAAGGggt                                           <  1:1183580/93‑1 (MQ=255)

                                                 aGCTAACGTACCGGCATTTGTTTCTGGCAAGGCACTGAAAGGCGCAGTTAAGTAAGATTGCGTGGCAGTGAACAGTTTTAACGAAGGggtggt                                        <  2:4385610/93‑1 (MQ=255)

                                                  gCTAACGTACCGGCATTTGTTTCTGGCAAGGCACTGAAAGGCGCAGTTAAGTAAGATTGCGTGGCAGTGAACATTTTTAACGAAGGGGTGGtt                                       <  1:6526843/93‑1 (MQ=255)

                                                  gCTAACGTACCGGCATTTGTTTCTGGCAAGGCACTGAAAGGCGCAGTTAAGTAAGATTGCGTGGCAGTGAACAGTTTTAACGAAGGGGTGGtt                                       >  1:6574331/1‑93 (MQ=255)

                                                  gCTAACGTACCGGCATTTGTTTCTGGCAAGGCACTGAAAGGCGCAGTTAAGTAAGATTGCGTGGCAGTGAACAGTTTTAACGAAGGGGTGGtt                                       >  2:1291321/1‑93 (MQ=255)

                                                  gCTAACGTACCGGCATTTGTTTCTGGCAAGGCACTGAAAGGCGCAGTTAAGTAAGATTGCGTGGCAGTGAACAGTTTTAACGAAGGGGTGGtt                                       <  2:3223512/93‑1 (MQ=255)

                                                    tAACGTACCGGCATTTGTTTCTGGCAAGGCACTGAAAGGCGCAGTTAAGTAAGATTGCGTGGCAGTGAACAGTTTTAACGAAGGGGTGGTTTc                                     >  1:2895849/1‑93 (MQ=255)

                                                     aaCGTACCGGCATTTGTTTCTGGCAAGGCACTGAAAGGCGCAGTTAAGTAAGATTGCGTGGCAGTGAACAGTTTTAACGAAGGGGTGGTTTCa                                    >  2:4046155/1‑93 (MQ=255)

                                                     aaCGTACCGGCATTTGTTTCTGGCAAGGCACTGAAAGGCGCAGTTAAGTAAGATTGCGTGGCAGTGAACAGTTTTAAAGAAGGGGTGGTTTCa                                    >  2:5041571/1‑93 (MQ=255)

                                                         tACCGGCATTTGTTTCTGGCAAGGCACTGAAAGGCGCAGTTAAGTAAGATTGCGTGGCAGTGAACAGTTTTAACGAAGGGGTGGTTTCAcccc                                >  1:4898847/1‑93 (MQ=255)

                                                         tACCGGCATTTGTTTCTGGCAAGGCACTGAAAGGCGCAGTTAAGTAAGATTGCGTGGCAGTGAACAGTTTTAACGAAGGGGTGGTTTCAcccc                                <  2:5297903/93‑1 (MQ=255)

                                                         tACCGGCATTTGTTTCTGGCAAGGCACTGAAAGGCGCAGTTAAGTAAGATTGCGTGGCAGTGAACAGTTTTAACGAAGGGGTGGTTTCAcccc                                <  1:2605456/93‑1 (MQ=255)

                                                              gCATTTGTTTCTGGCAAGGCACTGAAAGGCGCAGTTAAGTAAGATTGCGTGGCAGTGAACAGTTTTAACGAAGGGGTGGTTTCACCCCTTTTg                           >  1:6336924/1‑93 (MQ=255)

                                                                aTTTGTTTCTGGCAAGGCACTGAAAGGCGCAGTTAAGTAAGATTGCGTGGCAGTGAACAGTTTTAACGAAGGGGTGGTTTCACCCCTTTTGTc                         >  1:3450034/1‑93 (MQ=255)

                                                                aTTTGTTTCTGGCAAGGCACTGAAAGGCGCAGTTAAGTAAGATTGCGTGGCAGTGAACAGTTTTAACGAAGGGGTGGTTTCACCCCTTTTGTc                         <  2:4318793/93‑1 (MQ=255)

                                                                 tttGTTTCTGGCAAGGCACTGAAAGGCGCAGTTAAGTAAGATTGCGTGGCAGTGAACAGTTTTAACGAAGGGGTGGTTTCACCCCTTTTGTCt                        >  1:1415154/1‑93 (MQ=255)

                                                                 tttGTTTCTGGCAAGGCACTGAAAGGCGCAGTTAAGTAAGATTGCGTGGCAGTGAACAGTTTTAACGAAGGGGTGGTTTCACCCCTTTTGTCt                        <  1:1863189/93‑1 (MQ=255)

                                                                   tGTTTCTGGCAAGGCACTGAAAGGCGCAGTTAAGTAAGATTGCGTGGCAGTGAACAGTTTTAACGAAGGGGTGGTTTCACCCCTTTTGTCttt                      >  1:5978532/1‑93 (MQ=255)

                                                                    gTTTCTGGCAAGGCACTGAAAGGCGCAGTTAAGTAAGATTGCGTGGCAGTGAACAGTTTTAACGAAGGGGTGGTTTCACCCCTTTTGTCTTTc                     <  1:2187247/93‑1 (MQ=255)

                                                                     tttCTGGCAAGGCACTGAAAGGCGCAGTTAAGTAAGATTGCGTGGCAGTGAACAGTTTTAACGAAGGGGTGGTTTCACCCCTTTTGTCTTTCt                    <  2:6034449/93‑1 (MQ=255)

                                                                       tCTGGCAAGGCACTGAAAGGCGCAGTTAAGTAAGATTGCGTGGCAGTGAACAGTTTTAACGAAGGGGTGGTTTCACCCCTTTTGTCTTTCTgg                  <  1:1262684/93‑1 (MQ=255)

                                                                        cTGGCAAGGCACTGAAAGGCGCAGTTAAGTAAGATTGCGTGGCAGTGAACAGTTTTAACGAAGGGGTGGTTTCACCCCTTTTGTCTTTCTGGc                 <  1:4336175/93‑1 (MQ=255)

                                                                        cTGGCAAGGCACTGAAAGGCGCAGTTAAGTAAGATTGCGTGGCAGTGAACAGTTTTAACGAAGGGGTGGTTTCACCCCTTTTGTCTTTCTGGc                 <  2:2843851/93‑1 (MQ=255)

                                                                         tGGCAAGGCACTGAAAGGCGCAGTTAAGTAAGATTGCGTGGCAGTGAACAGTTTTAACGAAGGGGTGGTTTCACCCCTTTTGTCTTTCTGGCg                >  1:980543/1‑93 (MQ=255)

                                                                          ggCAAGGCACTGAAAGGCGCAGTTAAGTAAGATTGCGTGGCAGTGAACAGTTTTAACGAAGGGGTGGTTTCACCCCTTTTGTCTTTCTGGCGt               <  1:1977250/93‑1 (MQ=255)

                                                                          ggCAAGGCACTGAAAGGCGCAGTTAAGTAAGATTGCGTGGCAGTGAACAGTTTTAACGAAGGGGTGGTTTCACCCCTTTTGTCTTTCTGGCGt               <  1:5237735/93‑1 (MQ=255)

                                                                             aaGGCACTGAAAGGCGCGGTTAAGTAAGATTGCGTGGCAGTGAACAGTTTTAACGAAGGGGGGGTTTCACCCCTTTTGTCTTTCTGGCGTCGa            >  1:5290161/1‑93 (MQ=255)

                                                                             aaGGCACTGAAAGGCGCAGTTAAGTAAGATTGCGTGGCAGTGAACAGTTTTAACGAAGGGGTGGTTTCACCCCTTTTGTCTTTCTGGCGTCGa            <  1:4443514/93‑1 (MQ=255)

                                                                              aGGCACTGAAAGGCGCAGTTAAGTAAGATTGCGTGGCAGTGAACAGTTTTAACGAAGGGGTGGTTTCACCCCTTTTGTCTTTCTGGCGTCGAt           >  2:1445099/1‑93 (MQ=255)

                                                                               ggCACTGAAAGGCGCAGTTAAGTAAGATTGCGTGGCAGTGAACAGTTTTAACGAAGGGGTGGTTTCACCCCTTTTGTCTTTCTGGCGTCGATc          >  2:4754175/1‑93 (MQ=255)

                                                                               ggCACTGAAAGGCGCAGTTAAGTAAGATTGCGTGGCAGTGAACAGTTTTAACGAAGGGGTGGTTTCACCCCTTTTGTCTTTCTGGCGTCGATc          >  2:2197166/1‑93 (MQ=255)

                                                                               ggCACTGAAAGGCGCAGTTAAGTAAGATTGCGTGGCAGTGAACAGTTTTAACGAAGGGGTGGTTTCACCCCTTTTGTCTTTCTGGCGTCGATc          >  2:3121637/1‑93 (MQ=255)

                                                                                  aCTGAAAGGCGCAGTTAAGTAAGATTGCGTGGCAGTGAACAGTTTTAACGAAGGGGTGGTTTCACCCCTTTTGTCTTTCTGGCGTCGATCAtt       >  1:2991272/1‑93 (MQ=255)

                                                                                  aCTGAAAGGCGCAGTTAAGTAAGATTGCGTGGCAGTGAACAGTTTTAACGAAGGGGTGGTTTCACCCCTTTTGTCTTTCTGGCGTCGATCAtt       <  2:6353401/93‑1 (MQ=255)

                                                                                  aCTGAAAGGCGCAGTTAAGTAAGATTGCGTGGCAGTGAACAGTTTTAACGAAGGGGTGGTTTCACCACTTTTGTCTTTCTGGCGTCGATCAtt       >  2:5756319/1‑93 (MQ=255)

                                                                                   cTGAAAGGCGCAGTTAAGTAAGATTGCGTGGCAGTGAACAGTTTTAACGAAGGGGTGGTTTCACCCCTTTTGTCTTTCTGGCGTCGATCATTg      <  2:5393527/93‑1 (MQ=255)

                                                                                    tGAAAGGCGCAGTTAAGTAAGATTGCGTGGCAGTGAACAGTTTTAACGAAGGGGTGGTTTCACCCCTTTTGTCTTTCTGGCGTCGATCATTGa     <  1:3718220/93‑1 (MQ=255)

                                                                                    tGAAAGGCGCAGTTAAGTAAGATTGCGTGGCAGTGAACAGTTTTAACGAAGGGGTGGTTTCACCCCTTTTGTCTTTCTGGCGTCGATCATTGa     >  2:4039280/1‑93 (MQ=255)

                                                                                    tGAAAGGCGCAGTTAAGTAAGATTGCGTGGCAGTGAACAGTTTTAACGAAGGGGTGGTTTCACCCCTTTTGTCTTTCTGGCGTCGATCATTGa     >  2:2222814/1‑93 (MQ=255)

                                                                                    tGAAAGGCGCAGTTAAGTAAGATTGCGTGGCAGTGAACAGTTTTAACGAAGGGGTGGTTTCACCCCTTTTGTCTTTCTGGCGTCGATCATTGa     <  1:3204859/93‑1 (MQ=255)

                                                                                     gAAAGGCGCAGTTAAGTAAGATTGCGTGGCAGTGAACAGTTTTAACGAAGGGGTGGTTTCACCCCTTTTGTCTTTCTGGCGTCGATCATTGAt    >  1:6145025/1‑93 (MQ=255)

                                                                                     gAAAGGCGCAGTTAAGTAAGATTGCGTGGCAGTGAACAGTTTTAACGAAGGGGTGGTTTCACCCCTTTTGTCTTTCTGGCGTCGATCATTGAt    <  1:2528418/93‑1 (MQ=255)

                                                                                     gAAAGGCGCAGTTAAGTAAGATTGCGTGGCAGTGAACAGTTTTAACGAAGGGGTGGTTTCACCCCTTTTGTCTTTCTGGCGTCGATCATTGAt    <  2:6659201/93‑1 (MQ=255)

                                                                                       aaGGCGCAGTTAAGTAAGATTGCGTGGCAGTGAACAGTTTTAACGAAGGGGTGGTTTCACCCCTTTTGTCTTTCTGGCGTCGATCATTGATgc  >  2:483120/1‑93 (MQ=255)

                                                                                       aaGGCGCAGTTAAGTAAGATTGCGTGGCAGTGAACAGTTTTAACGAAGGGGTGGTTTCACCCCTTTTGTCTTTCTGGCGTCGATCATTGATgc  >  2:5579442/1‑93 (MQ=255)

                                                                                       aaGGCGCAGTTAAGTAAGATTGCGTGGCAGTGAACAGTTTTAACGAAGGGGTGGTTTCACCCCTTTTGTCTTTCTGGCGTCGATCATTGATgc  >  1:5652117/1‑93 (MQ=255)

AGCGTACTGGCCGCAACCCGCAGACCGGTAAAGAAATCAAAATTGCCGCAGCTAACGTACCGGCATTTGTTTCTGGCAAGGCACTGAAAGACGCAGTTAAGTAAGATTGCGTGGCAGTGAACAGTTTTAACGAAGGGGTGGTTTCACCCCTTTTGTCTTTCTGGCGTCGATCATTGATGC  >  NC_000913/4198028‑4198207

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 23 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`