BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,203,421	A→G	V30A (GTT→GCT)	purD ←	phosphoribosylglycinamide synthetase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,203,421	0	A	G	100.0%	792.9 / NA	240	V30A (GTT→GCT)	purD	phosphoribosylglycinamide synthetase
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (128/112); total (128/112)

Only 100 of 255 total aligned reads displayed.

GCGAAATCCAACAGCGCCGGGATATCGGTCACGCCAATAGCAACGTTTTGCAGCGCGGGTTCCAGTGCAGTGCCTGCATTACCCGGAGCAACAAAAACAGTCTCAACCAGCGGCGACTGGGCCGCTTTCCAGGCCAGCGCGTGCTCGCGCCCGCCGTTACCAATCACTAATACTTTCATC  >  NC_000913/4203331‑4203510

 cGAAATCCAACAGCGCCGGGATATCGGTCACGCCAATAGCAACGTTTTGCAGCGCGGGTTCCAGTGCAGTGCCTGCATTACCCGGAgcagcac                                                                                        <  2:838917/93‑2 (MQ=255)

 cGAAATCCAACAGCGCCGGGATATCGGTCACGCCAATAGCAACGTTTTGCAGCGCGGGTTCCAGTGCAGTGCCTGCATTACCCGGAGcagcaa                                                                                        <  1:3953019/93‑1 (MQ=255)

    aaTCCAACAGCGCCGGGATATCGGTCACGCCAATAGCAACGTTTTGCAGCGCGGGTTCCAGTGCAGTGCCTGCATTACCCGGAGCAGCaaaaa                                                                                     >  2:6340364/1‑93 (MQ=255)

    aaTCCAACAGCGCCGGGATATCGGTCACGCCAATAGCAACGTTTTGCAGCGCGGGTTCCAGTGCAGTGCCTGCATTACCCGGAGCAGCaaaaa                                                                                     <  2:1857541/93‑1 (MQ=255)

     aTCCAACAGCGCCGGGATATCGGTCACGCCAATAGCAACGTTTTGCAGCGCGGGTTCCAGTGCAGTGCCTGCATTACCCGGAGCAGCAAAAAc                                                                                    >  1:6015461/1‑93 (MQ=255)

      tCCAACAGCGCCGGGATATCGGTCACGCCAATAGCAACGTTTTGCAGCGCGGGTTCCAGTGCAGTGCCTGCATTTCCCGGAGCAGCAAAAACa                                                                                   <  1:423596/93‑1 (MQ=255)

      gccAACAGCGCCGGGATATCGGTCACGCCAATAGCAACGTTTTGCAGCGCGGGTTCCAGTGCAGTGCCTGCATTACCCGGAGCAGCAAAAACa                                                                                   <  1:6640450/92‑1 (MQ=255)

      accAACAGCGCCGGGATATCGGTCACGCCAATAGCAACGTTTTGCAGCGCGGGTTCCAGTGCAGTGCCTGCATTACCCGGAGCAGCAAAAACa                                                                                   <  2:6168940/92‑1 (MQ=255)

       ccAACAGCGCCGGGATATCGGTCACGCCAATAGCAACGTTTTGCAGCGCGGGTTCCAGTGCAGTGCCTGCATTACCCGGAGCAGCAAAAACAg                                                                                  <  1:557165/93‑1 (MQ=255)

        cAACAGCGCCGGGATATCGGTCACGCCAATAGCAACGTTTTGCAGCGCGGGTTCCAGTGCAGTGCCTGCATTACCCGGAGCAGCAAAAACAGt                                                                                 >  1:811653/1‑93 (MQ=255)

        cAACAGCGCCGGGATATCGGTCACGCCAATAGCAACGTTTTGCAGCGCGGGTTCCAGTGCAGTGCCTGCATTACCCGGAGCAGCAAAAACAGt                                                                                 >  1:2449004/1‑93 (MQ=255)

        cAACAGCGCCGGGATATCGGTCACGCCAATAGCAACGTTTTGCAGCGCGGGTTCCAGTGCAGTGCCTGCATTACCCGGAGCAGCAAAAACAGt                                                                                 >  1:3025292/1‑93 (MQ=255)

         aaCAGCGCCGGGATATCGGTCACGCCAATAGCAACGTTTTGCAGCGCGGGTTCCAGTGCAGTGCCTGCATTACCCGGAGCAGCAAAAACAGtc                                                                                <  1:4377475/93‑1 (MQ=255)

         aaCAGCGCCGGGATATCGGTCACGCCAATAGCAACGTTTTGCAGCGCGGGTTCCAGTGCAGTGCCTGCATTACCCGGAGCAGCAAAAACAGtc                                                                                <  1:3503647/93‑1 (MQ=255)

          aCAGCGCCGGGATATCGGTCACGCCAATAGCAACGTTTTGCAGCGCGGGTTCCAGTGCAGTGCCTGCATTACCCGGAGCAGCAAAAACAGtct                                                                               <  2:4894160/93‑1 (MQ=255)

          aCAGCGCCGGGATATCGGTCACGCCAATAGCAACGTTTTGCAGCGCGGGTTCCAGTGCAGTGCCTGCATTACCCGGAGCAGCAAAAACAGtct                                                                               >  2:3702053/1‑93 (MQ=255)

           cAGCGCCGGGATATCGGTCACGCCAATAGCAACGTTTTGCAGCGCGGGTTCCAGTGCAGTGCCTGCATTACCCGGAGCAGCAAAAACAGtctc                                                                              <  1:2921412/93‑1 (MQ=255)

           cAGCGCCGGGATATCGGTCACGCCAATAGCAACGTTTTGCAGCGCGGGTTCCAGTGCAGTGCCTGCATTACCCGGAGCAGCAAAAACAGtctc                                                                              >  2:1153772/1‑93 (MQ=255)

           cAGCGCCGGGATATCGGTCACGCCAATAGCAACGTTTTGCAGCGCGGGTTCCAGTGCAGTGCCTGCATTACCCGGAGCAGCAAAAACAGtctc                                                                              >  1:1359640/1‑93 (MQ=255)

           cAGCGCCGGGATATCGGTCACGCCAATAGCAACGTTTTGCAGCGCGGGTTCCAGTGCAGTGCCTGCATTACCCGGAGCAGCAAAAACAGtctc                                                                              <  2:4028620/93‑1 (MQ=255)

            aGCGCCGGGATATCGGTCACGCCAATAGCAACGTTTTGCAGCGCGGGTTCCAGTGCAGTGCCTGCATTACCCGGAGCAGCAAAAACAGTCTCa                                                                             >  2:1197760/1‑93 (MQ=255)

             gcgcCGGGATATCGGTCACGCCAATAGCAACGTTTTGCAGCGCGGGTTCCAGTGCAGTGCCTGCATTACCCGGAGCAGCAAAAACAGTCTCaa                                                                            >  2:5515343/1‑93 (MQ=255)

             gcgcCGGGATATCGGTCACGCCAATAGCAACGTTTTGCAGCGCGGGTTCCAGTGCAGTGCCTGCATTACCCGGAGCAGCAAAAACAGTCTCaa                                                                            <  1:3283872/93‑1 (MQ=255)

               gcCGGGATATCGGTCACGCCAATAGCAACGTTTTGCAGCGCGGGTTCCAGTGCAGTGCCTGCATTACCCGGAGCAGCAAAAACAGTCTCAAcc                                                                          <  1:3652608/93‑1 (MQ=255)

                ccGGGATATCGGTCACGCCAATAGCAACGTTTTGCAGCGCGGGTTCCAGTGCAGTGCCTGCATTACCCGGAGCAGCAAAAACAGTCTCAACCa                                                                         <  2:315188/93‑1 (MQ=255)

                   ggATATCGGTCACGCCAATAGCAACGTTTTGCAGCGCGGGTTCCAGTGCAGTGCCTGCATTACCCGGAGCAGCAAAAACAGTCTCAACCAgcg                                                                      >  1:6109608/1‑93 (MQ=255)

                    gATATCGGTCACGCCAATAGCAACGTTTTGCAGCGCGGGTTCCAGTGCAGTGCCTGCATTACCCGGAGCAGCAAAAACAGTCTCAACCAgcgg                                                                     <  2:2770576/93‑1 (MQ=255)

                     atatCGGTCACGCCAATAGCAACGTTTTGCAGCGCGGGTTCCAGTGCAGTGCCTGCATTACCCGGAGCAGCAAAAACAGTCTCAACCAgcggc                                                                    <  1:1596235/93‑1 (MQ=255)

                      tatCGGTCACGCCAATAGCAACGTTTTGCAGCGCGGGTTCCAGTGCAGTGCCTGCATTACCCGGAGCAGCAAAAACAGTCTCAACCAgcggcg                                                                   <  2:6024687/93‑1 (MQ=255)

                       atCGGTCACGCCAATAGCAACGTTTTGCAGCGCGGGTTCCAGTGCAGTGCCTGCATTACCCGGAGCAGCAAAAACAGTCTCAACCAGCGGCGa                                                                  <  1:5501998/93‑1 (MQ=255)

                       atCGGTCACGCCAATAGCAACGTTTTGCAGCGCGGGTTCCAGTGCAGTGCCTGCATTACCCGGAGCAGCAAAAACAGTCTCAACCAGCGGCGa                                                                  <  2:1549314/93‑1 (MQ=255)

                       atCGGTCACGCCAATAGCAACGTTTTGCAGCGCGGGTTCCAGTGCAGAGCCTGCATTACCCGGAGCAGCAAAAACAGTCTCAACCAGCAGCGa                                                                  >  1:6274067/1‑93 (MQ=255)

                        tCGGTCACGCCAATAGCAACGTTTTGCAGCGCGGGTTCCAGTGCCGTGCCTGCATTACCCGGAGCCGCAAAAACAGGCTCAACCAGCGGCGAc                                                                 >  1:1751425/1‑93 (MQ=255)

                         cGGTCACGCCAATAGCAACGTTTTGCAGCGCGGGTTCCAGTGCAGTGCCTGCATTACCCGGAGCAGCAAAAACAGTCTCAACCAGCGGCGACt                                                                <  2:2763680/93‑1 (MQ=255)

                          ggTCACGCCAATAGCAACGTTTTGCAGCGCGGGTTCCAGTGCAGTGCCTGCATTACCCGGAGCAGCAAAAACAGTCTCAACCAGCGGCGACTg                                                               <  1:2615980/93‑1 (MQ=255)

                          ggTCACGCCAATAGCAACGTTTTGCAGCGCGGGTTCCAGTGCAGTGCCTGCATTACCCGGAGCAGCAAAAACAGTCTCAACCAGCGGCGACTg                                                               >  2:5299606/1‑93 (MQ=255)

                             cACGCCAATAGCAACGTTTTGCAGCGCGGGTTCCAGTGCAGTGCCTGCATTACCCGGAGCAGCAAAAACAGTCTCAACCAGCGGCGACTGGGc                                                            <  2:4142553/93‑1 (MQ=255)

                               cGCCAATAGCAACGTTTTGCAGCGCGGGTTCCAGTGCAGTGCCTGCATTACCCGGAGCAGCAAAAACAGTCTCAACCAGCGGCGACTGGGCCg                                                          >  1:5683131/1‑93 (MQ=255)

                                 ccAATAGCAACGTTTTGCAGCGCGGGTTCCAGTGCAGTGCCTGCATTACCCGGAGCAGCAAAAACAGTCTCAACCAGCGGCGACTGGGCCGCt                                                        <  2:3356332/93‑1 (MQ=255)

                                  cAATAGCAACGTTTTGCAGCGCGGGTTCCAGTGCAGTGCCTGCATTACCCGGAGCAGCAAAAACAGTCTCAACCAGCGGCGACTGGGCCGCtt                                                       <  2:2617547/93‑1 (MQ=255)

                                   aaTAGCAACGTTTTGCAGCGCGGGTTCCAGTGCAGTGCCTGCATTACCCGGAGCAGCAAAAACAGTCTCAACCAGCGGTGACTGAGCCGCttt                                                      >  1:3797316/1‑93 (MQ=255)

                                   aaTAGCAACGTTTTGCAGCGCGGGTTCCAGTGCAGTGCCTGCATTACCCGGAGCAGCAAAAACAGTCTCAACCAGCGGCGACTGGGCCGCttt                                                      >  1:4311653/1‑93 (MQ=255)

                                    aTAGCAACGTTTTGCAGCGCGGGTTCCAGTGCAGTGCCTGCATTACCCGGAGCAGCAAAAACAGTCTCAACCAGCGGCGACTGGGCCGCTTTc                                                     <  2:4541310/93‑1 (MQ=255)

                                    aTAGCAACGTTTTGCAGCGCGGGTTCCAGTGCAGTGCCTGCATTACCCGGAGCAGCAAAAACAGTCTCAACCAGCGGCGACTGGGCCGCTTTc                                                     >  2:788398/1‑93 (MQ=255)

                                     tAGCAACGTTTTGCAGCGCGGGTTCCAGTGCAGTGCCTGCATTACCCGGAGCAGCAAAAACAGTCTCAACCAGCGGCGACTGGGCCGCTTTcc                                                    <  1:2805674/93‑1 (MQ=255)

                                     tAGCAACGTTTTGCAGCGCGGGTTCCAGTGCAGTGCCTGCATTACCCGGAGCAGCAAAAACAGTCTCAACCAGCGGCGACTGGGCCGCTTTcc                                                    <  1:267390/93‑1 (MQ=255)

                                     tAGCAACGTTTTGCAGCGCGGGTTCCAGTGCAGTGCCTGCATTACCCGGAGCAGCAAAAACAGTCTCAACCAGCGGCGACTGGGCCGCTTTcc                                                    <  1:30903/93‑1 (MQ=255)

                                     tAGCAACGTTTTGCAGCGCGGGTTCCAGTGCAGTGCCTGCATTACCCGGAGCAGCAAAAACAGTCTCAACCAGCGGCGACTGGGCCGCTTTcc                                                    <  1:3275290/93‑1 (MQ=255)

                                      aGCAACGTTTTGCAGCGCGGGTTCCAGTGCAGTGCCTGCATTACCCGGAGCAGCAAAAACAGTCTCAACCAGCGGCGACTGGGCCGCTTTCCa                                                   >  1:4755346/1‑93 (MQ=255)

                                       gCAACGTTTTGCAGCGCGGGTTCCAGTGCAGTGCCTGCATTACCCGGAGCAGCAAAAACAGTCTCAACCAGCGGCGACTGGGCCGCTTTCCAg                                                  >  1:2228620/1‑93 (MQ=255)

                                       gCAACGTTTTGCAGCGCGGGTTCCAGTGCAGTGCCTGCATTACCCGGAGCAGCAAAAACAGTCTCAACCAGCGGCGACTGGGCCGCTTTCCAg                                                  >  2:2520679/1‑93 (MQ=255)

                                       gCAACGTTTTGCAGCGCGGGTTCCAGTGCAGTGCCTGCATTACCCGGAGCAGCAAAAACAGTCTCAACCAGCGGCGACTGGGCCGCTTTCCAg                                                  <  1:1938630/93‑1 (MQ=255)

                                       gCAACGTTTTGCAGCGCGGGTTCCAGTGCAGTGCCTGCATTACCCGGAGCAGCAAAAACAGTCTCAACCAGCGGCGACTGGGCCGCTTTCCAg                                                  >  2:4252519/1‑93 (MQ=255)

                                       gCAACGTTTTGCAGCGCGGGTTCCAGTGCAGTGCCTGCATTACCCGGAGCAGCAAAAACAGTCTCAACCAGCGGCGACTGAGCCGCTTTCCAg                                                  >  2:5012827/1‑93 (MQ=255)

                                         aaCGTTTTGCAGCGCGGGTTCCAGTGCAGTGCCTGCATTACCCGGAGCAGCAAAAACAGTCTCAACCAGCGGCGACTGGGCCGCTTTCCAGGc                                                >  2:2921933/1‑93 (MQ=255)

                                             ttttGCAGCGCGGGTTCCAGTGCAGTGCCTGCATTACCCGGAGCAGCAAAAACAGTCTCAACCAGCGGCGACTGGGCCGCTTTCCAGGCCAgc                                            >  1:1193534/1‑93 (MQ=255)

                                              tttGCAGCGCGGGTTCCAGTGCAGTGCCTGCATTACCCGGAGCAGCAAAAACAGTCTCAACCAGCGGCGACTGGGCCGCTTTCCAGGCCAgcg                                           >  2:6651186/1‑93 (MQ=255)

                                               ttGCAGCGCGGGTTCCAGTGCAGTGCCTGCATTACCCGGAGCAGCAAAAACAGTCTCAACCAGCGGCGACTGGGCCGCTTTCCAGGCCAgcgc                                          >  2:9704/1‑93 (MQ=255)

                                                 gCAGCGCGGGTTCCAGTGCAGTGCCTGCATTACCCGGAGCAGCAAAAACAGTCTCAACCAGCGGCGACTGGGCCGCTTTCCAGGCCAGCGCGt                                        >  1:485943/1‑93 (MQ=255)

                                                  cAGCGCGGGTTCCAGTGCAGTGCCTGCATTACCCGGAGCAGCAAAAACAGTCTCAACCAGCGGCGACTGGGCCGCTTTCCAGGCCAGCGCGTg                                       >  1:1556287/1‑93 (MQ=255)

                                                   aGCGCGGGTTCCAGTGCAGTGCCTGCATTACCCGGAGCAGCAAAAACAGTCTCAACCAGCGGCGACTGGGCCGCTTTCCAGGCCAGCGCGTGc                                      >  2:222264/1‑93 (MQ=255)

                                                   aGCGCGGGTTCCAGTGCAGTGCCTGCATTACCCGGAGCAGCAAAAACAGTCTCAACCAGCGGCGACTGGGCCGCTTTCCAGGCCAGCGCGTGc                                      <  2:344576/93‑1 (MQ=255)

                                                      gcgGGTTCCAGTGCAGTGCCTGCATTACCCGGAGCAGCAAAAACAGTCTCAACCAGCGGCGACTGGGCCGCTTTCCAGGCCAGCGCGTGCTcg                                   <  1:6338154/93‑1 (MQ=255)

                                                        gggTTCCAGTGCAGTGCCTGCATTACCCGGAGCAGCAAAAACAGTCTCAACCAGCGGCGACTGGGCCGCTTTCCAGGCCAGCGCGTGCTcgcg                                 >  1:2946311/1‑93 (MQ=255)

                                                        gggTTCCAGTGCAGTGCCTGCATTACCCGGAGCAGCAAAAACAGTCTCAACCAGCGGCGACTGGGCCGCTTTCCAGGCCAGCGCGTGCTcgcc                                 >  2:5819803/1‑92 (MQ=255)

                                                          gTTCCAGTGCAGTGCCTGCATTACCCGGAGCAGCAAAAACAGTCTCAACCAGCGGCGACTGGGCCGCTTTCCAGGCCAGCGCGTGCTCGcgcc                               <  1:447366/93‑1 (MQ=255)

                                                           ttCCAGTGCAGTGCCTGCATTACCCGGAGCAGCAAAAACAGTCTCAACCAGCGGCGACTGGGCCGCTTTCCAGGCCAGCGCGTGCTCGcgccc                              >  1:2525535/1‑93 (MQ=255)

                                                             ccAGTGCAGTGCCTGCATTACCCGGAGCAGCAAAAACAGTCTCAACCAGCGGCGACTGGGCCGCTTTCCAGGCCAGCGCGTGCTCGcgcccgc                            <  1:6373144/93‑1 (MQ=255)

                                                             ccAGTGCAGTGCCTGCATTACCCGGAGCAGCAAAAACAGTCTCAACCAGCGGCGACTGGGCCGCTTTCCAGGCCAGCGCGTGCTCGcgcccgc                            <  1:5208768/93‑1 (MQ=255)

                                                               agtgcagtgcCTGCATTACCCGGAGCAGCAAAAACAGTCTCAACCAGCGGCGACTGGGCCGCTTTCCAGGCCAGCGCGTGCTCGCGCccgccg                          >  1:4917509/1‑93 (MQ=255)

                                                               agtgcagtgcCTGCATTACCCGGAGCAGCAAAAACAGTCTCAACCAGCGGCGACTGGGCCGCTTTCCAGGCCAGCGCGTGCTCGCGCccgccg                          <  2:5688909/93‑1 (MQ=255)

                                                                gtgcagtgcCTGCATTACCCGGAGCAGCAAAACCAGTCTCAACCAGCGGCGACTGGGCCGCTTTCCAGGCCAGCGCGTGCTCGCGCCCGCCGt                         <  1:4117479/93‑1 (MQ=255)

                                                                gtgcagtgcCTGCATTACCCGGAGCAGCAAAAACAGTCTCAACCAGCGGCGACTGGGCCGCTTTCCAGGCCAGCGCGTGCTCGCGCCCGCCGt                         <  2:4794050/93‑1 (MQ=255)

                                                                gtgcagtgcCTGCATTACCCGGAGCAGCAAAAACAGTCTCAACCAGCGGCGACTGGGCCGCTTTCCAGGCCAGCGCGTGCTCGCGCCCGCCGt                         >  1:931093/1‑93 (MQ=255)

                                                                gtgcagtgcCTGCATTACCCGGAGCAGCAAAAACAGTCTCAACCAGCGGCGACTGGGCCGCTTTCCAGGCCAGCGCGTGCTCGCGCCCGCCGt                         >  2:5745425/1‑93 (MQ=255)

                                                                 tgcagtgcCTGCATTACCCGGAGCAGCAAAAACAGTCTCAACCAGCGGCGACTGGGCCGCTTTCCAGGCCAGCGCGTGCTCGCGCCCGCCGtt                        <  2:205261/93‑1 (MQ=255)

                                                                    agtgcCTGCATTACCCGGAGCAGCAAAAACAGTCTCAACCAGCGGCGACTGGGCCGCTTTCCAGGCCAGCGCGTGCTCGCGCCCGCCGTTAcc                     <  2:4357005/93‑1 (MQ=255)

                                                                     gtgcCTGCATTACCCGGAGCAGCAAAAACAGTCTCAACCAGCGGCGACTGGGCCGCTTTCCAGGCCAGCGCGTGCTCGCGCCCGCCGTTACCa                    <  2:3973233/93‑1 (MQ=255)

                                                                     gtgcCTGCATTACCCGGAGCAGCAAAAACAGTCTCAACCAGCGGCGACTGGGCCGCTTTCCAGGCCAGCGCGTGCTCGCGCCCGCCGTTACCa                    >  1:1630355/1‑93 (MQ=255)

                                                                     gtgcCTGCATTACCCGGAGCAGCAAAAACAGTCTCAACCAGCGGCGACTGGGCCGCTTTCCAGGCCAGCGCGTGCTCGCGCCCGCCGTTACCa                    >  1:5373781/1‑93 (MQ=255)

                                                                     gtgcCTGCATTACCCGGAGCAGCAAAAACAGTCTCAACCAGCGGCGACTGGGCCGCTTTCCAGGCCAGCGCGTGCTCGCGCCCGCCGTTACCa                    >  1:5724227/1‑93 (MQ=255)

                                                                     gtgcCTGCATTACCCGGAGCAGCAAAAACAGTCTCAACCAGCGGCGACTGGGCCGCTTTCCAGGCCAGCGCGTGCTCGCGCCCGCCGTTACCa                    >  1:6704272/1‑93 (MQ=255)

                                                                      tgcCTGCATTACCCGGAGCAGCAAAAACAGTCTCAACCAGCGGCGACTGGGCCGCTTTCCAGGCCAGCGCGTGCTCGCGCCCGCCGTTACCaa                   <  1:1116380/93‑1 (MQ=255)

                                                                      ggcCGGCATTACCGGGAGCAGCAAAAACAGTCTCACCCAGCGGCGACTGGGCCGCTTTCCAGGCCAGCGCGTGCTCGCGCCCGCCGTTACCaa                   <  2:4963684/92‑1 (MQ=255)

                                                                        ccTGCATTACCCGGAGCAGCAAAAACAGTCTCAACCAGCGGCGACTGGGCCGCTTTCCAGGCCAGCGCGTGCTCGCGCCCGCCGTTACCAATc                 <  2:3548017/93‑1 (MQ=255)

                                                                         cTGCATTACCCGGAGCAGCAAAAACAGTCTCAACCAGCGGCGACTGGGCCGCTTTCCAGTGCAGCGCGTGCTCGCGCCCGCCGCTACCAATCa                >  1:5314486/1‑93 (MQ=255)

                                                                         cTGCATTACCCGGAGCAGCAAAAACAGTCTCAACCAGCGGCGACTGGGCCGCTTTCCAGGCCAGCGCGTGCTCGCGCCCGCCGTTACCAATCa                <  2:507681/93‑1 (MQ=255)

                                                                         cTGCATTACCCGGAGCAGCAAAAACAGTCTCAACCAGCGGCGACTGGGCCGCTTTCCAGGCCAGCGCGTGCTCGCGCCCGCCGTTACCAATCa                <  2:5948528/93‑1 (MQ=255)

                                                                         cTGCATTACCCGGAGCAGCAAAAACAGTCTAAACCAGCGGCGACTGGGCCGCTTTCCAGGCCAGCGCGTGCTCGCGCCCGCCGTTACCAATCa                <  2:1576353/93‑1 (MQ=255)

                                                                          tGCATTACCCGGAGCAGCAAAAACAGTCTCAACCAGCGGCGACTGGGCCGCTTTCCAGGCCAGCGCGTGCTCGCGCCCGCCGTTACCAATCAc               >  2:5200759/1‑93 (MQ=255)

                                                                          tGCATTACCCGGAGCAGCAAAAACAGTCTCAACCAGCGGCGACTGGGCCGCTTTCCAGGCCAGCGCGTGCTCGCGCCCGCCGTTACCAATCAc               <  1:1337855/93‑1 (MQ=255)

                                                                          tGCATTACCCGGAGCAGCAAAAACAGTCTCAACCAGCGGCGACTGGGCCGCTTTCCAGGCCAGCGCGTGCTCGCGCCCGCCGTTACCAATCAc               >  2:6472707/1‑93 (MQ=255)

                                                                            cATTACCCGGAGCAGCAAAAACAGTCTCAACCAGCGGCGACTGGGCCGCTTTCCAGGCCAGCGCGTGCTCGCGCCCGCCGTTACCAATCACTa             <  2:5422466/93‑1 (MQ=255)

                                                                              ttACCCGGAGCAGCAAAAACAGTCTCAACCAGCGGAGACTGGGCCGCTTTCCAGGCCAGCGCGTGCTCGCGCCCGCCGTTACCAATCACTAAt           >  2:3822606/1‑93 (MQ=255)

                                                                               tACCCGGAGCAGCAAAAACAGTCTCACCCAGCGGCGACTGGGCCGCTTTCCAGGCCAGCGCGTGCTCGCGCCCGCCGTTACCAATCACTAATa          <  2:1679430/93‑1 (MQ=255)

                                                                               tACCCGGAGCAGCAAAAACAGTCTCAACCAGCGGCGACTGGGCCGCTTTCCAGGCCAGCGCGTGCTCGCGGCCGCCGTTACCAATCACTAATa          >  1:585053/1‑93 (MQ=255)

                                                                               tACCCGGAGCAGCAAAAACAGTCTCAACCAGCGGCGACTGGGCCGCTTTCCAGGCCAGCGCGTGCTCGCGCCCGCCGTTACCAATCACTAATa          >  1:2008962/1‑93 (MQ=255)

                                                                                  ccGGAGCAGCAAAAACAGTCTCAACCAGCGGCGACTGGGCCGCTTTCCAGGCCAGCGCGTGCTCGCGCCCGCCGTTACCAATCACTAATACtt       <  1:992181/93‑1 (MQ=255)

                                                                                    ggAGCAGCAAAAACAGTCTCAACCAGCGGCGACTGGGCCGCTTTCCAGGCCAGCGCGTGCTCGCGCCCGCCGTTACCAATCACTAATACTTTc     >  2:2409567/1‑93 (MQ=255)

                                                                                       gcagcaAAAACAGTCTCAACCAGCGGCGACTGGGCCGCTTTCCAGGCCAGCGCGTGCTCGCGCCCGCCGTTACCAATCACTAATACTTTCATc  >  1:4803286/1‑93 (MQ=255)

GCGAAATCCAACAGCGCCGGGATATCGGTCACGCCAATAGCAACGTTTTGCAGCGCGGGTTCCAGTGCAGTGCCTGCATTACCCGGAGCAACAAAAACAGTCTCAACCAGCGGCGACTGGGCCGCTTTCCAGGCCAGCGCGTGCTCGCGCCCGCCGTTACCAATCACTAATACTTTCATC  >  NC_000913/4203331‑4203510

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 17 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`