BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,216,494	A→G	Y107C (TAC→TGC)	aceK →	isocitrate dehydrogenase kinase/phosphatase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,216,494	0	A	G	100.0%	787.8 / NA	244	Y107C (TAC→TGC)	aceK	isocitrate dehydrogenase kinase/phosphatase
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (113/131); total (113/131)

Only 100 of 249 total aligned reads displayed.

CATTTTTACTACGTGTTAAAGAGCATTACACCCGGCTGTTGCCGGATTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGTGTACTGTCGGTTATTTGACCACCGCTCGCTTACTCCCGAGCGGCTTTTTATCTTTAGCTCTCAGCCAGAGCGCCGCTTTCGTACCATTCCC  >  NC_000913/4216404‑4216582

cATTTTTACTACGTGTTAAAGAGCATTACACCCGGCTGTTGCCGGATTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGTGTGCt                                                                                        >  2:3869893/1‑93 (MQ=255)

  tttttACTACGTGTTAAAGAGCATTACACCCGGCTGTTGCCGGATTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGTGTGCTGt                                                                                      <  1:4141603/93‑1 (MQ=255)

  tttttACTACGTGTTAAAGAGCATTACACCCGGCTGTTGCCGGATTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGTGTGCTGt                                                                                      <  1:169151/93‑1 (MQ=255)

      tactacGTGTTAAAGAGCATTACACCCGGCTGTTGCCGGATTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGTGTGCTGTCGGt                                                                                  <  2:2076526/93‑1 (MQ=255)

       actacGTGTTAAAGAGCATTACACCCGGCTGTTGCCGGATTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGTGTGCTGTCGGtt                                                                                 <  2:6689808/93‑1 (MQ=255)

        ctacGTGTTAAAGAGCATTACACCCGGCTGTTGCCGGATTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGTGTGCTGTCGGTTa                                                                                >  2:5625186/1‑93 (MQ=255)

          acGTGTTAAAGAGCATTACACCCGGCTGTTGCCGGATTACCCGCGCTTCGCGATTGCGGAGAGCTTTTTTAACTCCGTGTGCTGTCGGTTAtt                                                                              >  1:5449033/1‑93 (MQ=255)

          acGTGTTAAAGAGCATTACACCCGGCTGTTGCCGGATTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGTGTGCTGTCGGTTAtt                                                                              <  2:553148/93‑1 (MQ=255)

          acGTGTTAAAGAGCATTACACCCGGCTGTTGCCGGATTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGTGTGCTGTCGGTTAtt                                                                              <  2:1959016/93‑1 (MQ=255)

           cGTGTTAAAGAGCATTACACCCGGCTGTTGCCGGATTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGTGTGCTGTCGGTTAttt                                                                             <  1:1934224/93‑1 (MQ=255)

            gtgtTAAAGAGCATTACACCCGGCTGTTGCCGGATTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGTGTGCTGTCGGTTATTTg                                                                            <  2:2548858/93‑1 (MQ=255)

              gtTAAAGAGCATTACACCCGGCTGTTGCCGGATTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGTGTGCTGTCGGTTATTTGac                                                                          <  2:3710730/93‑1 (MQ=255)

              gtTAAAGAGCATTACACCCGGCTGTTGCCGGATTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGTGTGCTGTCGGTTATTTGac                                                                          >  2:5345595/1‑93 (MQ=255)

               ttAAAGAGCATTACACCCGGCTGTTGCCGGATTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGTGTGCTGTCGGTTATTTGacc                                                                         <  1:1173086/93‑1 (MQ=255)

                tAAAGAGCATTACACCCGGCTGTTGCCGGATTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGTGTGCTGTCGGTTATTTGacca                                                                        <  2:5193052/93‑1 (MQ=255)

                  aaGAGCATTACACCCGGCTGTTGCCGGATTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGTGTGCTGTCGGTTATTTGaccacc                                                                      >  2:946868/1‑93 (MQ=255)

                   agagCATTACACCCGGCTGTTGCCGGATTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGTGTGCTGTCGGTTATTTGACCACcg                                                                     >  1:1994147/1‑93 (MQ=255)

                   agagCATTACACCCGGCTGTTGCCGGATTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGTGTGCTGTCGGTTATTTGACCACcg                                                                     >  1:734809/1‑93 (MQ=255)

                    gagCATTACACCCGGCTGTTGCCGGATTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGTGTGCTGTCGGTTATTTGACCACcgc                                                                    >  2:2795716/1‑93 (MQ=255)

                    gagCATTACACCCGGCTGTTGCCGGATTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGTGTGCTGTCGGTTATTTGACCACcgc                                                                    >  2:1329402/1‑93 (MQ=255)

                    gagCATTACACCCGGCTGTTGCCGGATTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGTGTGCTGTCGGTTATTTGACCACcgc                                                                    <  1:43929/93‑1 (MQ=255)

                    gagCATTACACCCGGCTGTTGCCGGATTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGTGTGCTGTCGGTTATTTGACCACcgc                                                                    >  1:129610/1‑93 (MQ=255)

                     agCATTACACCCGGCTGTTGCCGGATTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGTGTGCTGTCGGTTATTTGACCACcgct                                                                   <  1:3577043/93‑1 (MQ=255)

                      gCATTACACCCGGCTGTTGCCGGATTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGTGTGCTGTCGGTTATTTGACCACcgctc                                                                  >  1:3233174/1‑93 (MQ=255)

                        aTTACACCCGGCTGTTGCCGGATTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGTGTGCTGTCGGTTATTTGACCACcgctcgc                                                                >  2:787522/1‑93 (MQ=255)

                        aTTACACCCGGCTGTTGCCGGATTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGTGTGCTGTCGGTTATTTGACCACcgctcgc                                                                >  2:3526320/1‑93 (MQ=255)

                         ttACACCCGGCTGTTGCCGGATTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGTGTGCTGTCGGTTATTTGACCACcgctcgct                                                               <  1:6671432/93‑1 (MQ=255)

                         ttACACCCGGCTGTTGCCGGATTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGTGTGCTGTCGGTTATTTGACCACcgctcgct                                                               <  1:5638828/93‑1 (MQ=255)

                            cacCCGGCTGTTGCCGGATTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGTGTGCTGTCGGTTATTTGACCACCGCTCGCTTAc                                                            <  1:2201524/93‑1 (MQ=255)

                            cacCCGGCTGTTGCCGGATTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGTGTGCTGTCGGTTATTTGACCACCGCTCGCTTAc                                                            >  2:1494647/1‑93 (MQ=255)

                             acCCGGCTGTTGCCGGATTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGTGTGCTGTCGGTTATTTGACCACCGCTCGCTTACt                                                           <  1:6216153/93‑1 (MQ=255)

                              cccGGCTGTTGCCGGATTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGTGTGCTGTCGGTTATTTGACCACCGCTCGCTTACTc                                                          >  1:2332165/1‑93 (MQ=255)

                                cGGCTGTTGCCGGATTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGTGTGCTGTCGGTTATTTGTCCACCGCTCGCTTACTccc                                                        <  1:6565941/93‑1 (MQ=255)

                                cGGCTGTTGCCGGATTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGTGTGCTGTCGGTTATTTGACCACCGCTCGCTTACTccc                                                        <  2:600581/93‑1 (MQ=255)

                                cGGCTGTTGCCGGATTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGTGTGCTGTCGGTTATTTGACCACCGCTCGCTTACTccc                                                        <  1:1828955/93‑1 (MQ=255)

                                cGGCTGTTGCCGGATTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGTGTGCTGTCGGTTATTTGACCACCGCTCGCTTACTccc                                                        <  1:4048339/93‑1 (MQ=255)

                                 ggCTGTTGCCGGATTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGTGTGCTGTCGGTTATTTGACCACCGCTCGCTTACTCCCg                                                       <  1:187192/93‑1 (MQ=255)

                                  gCTGTTGCCGGATTACCCGCGCTTCGAGATTGCGGAGAGCATTTTTAACTCCGTGTGCTGTCGGTTATTTGACCACCGCTCGCTTACTCCCGa                                                      <  1:4246960/93‑1 (MQ=255)

                                    tGTTGCCGGATTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGTGTGCTGTCGGTTATTTGACCACCGCTCGCTTACTCCCGAGc                                                    >  2:2057046/1‑93 (MQ=255)

                                    tGTTGCCGGATTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGTGTGCTGTCGGTTATTTGACCACCGCTCGCTTACTCCCGAGc                                                    >  2:5430656/1‑93 (MQ=255)

                                    tGTTGCCGGATTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGTGTGCTGTCGGTTATTTGACCACCGCTCGCTTACTCCCGAGc                                                    <  1:2900858/93‑1 (MQ=255)

                                    tGTTGCCGGATTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGTGTGCTGTCGGTTATTTGACCACCGCTCGCTTACTCCCGAGc                                                    <  1:9362/93‑1 (MQ=255)

                                     gTTGCCGGATTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGTGTGCTGTCGGTTATTTGACCACCGCTCGCTTACTCCCGAGCg                                                   >  1:6446447/1‑93 (MQ=255)

                                     gTTGCCGGATTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGTGTGCTGTCGGTTATTTGACCACCGCTCGCTTACTCCCGAGCg                                                   >  2:4095859/1‑93 (MQ=255)

                                     gTTGCCGGATTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGTGTGCTGTCGGTTATTTGACCACCGCTCGCTTACTCCCGAGCg                                                   >  2:1872551/1‑93 (MQ=255)

                                        gCCGGATTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGTGTGCTGTCGGTTATTTGACCACCGCTCGCTTACTCCCGAGCGGCt                                                <  2:1306589/93‑1 (MQ=255)

                                        gCCGGATTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGTGTGCTGTCGGTTATTTGACCACCGCTCGCTTACTCCCGAGCGGCt                                                <  2:5450485/93‑1 (MQ=255)

                                        gCCGGATTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGTGTGCTGTCGGTTATTTGACCACCGCTCGCTTACTCCCGAGCGGCt                                                <  1:1468233/93‑1 (MQ=255)

                                         ccGGATTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGTGTGCTGTCGGTTATTTGACCACCGCTCGCTTACTCCCGAGCGGCtt                                               <  2:658107/93‑1 (MQ=255)

                                            gATTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGTGTGCTGTCGGTTATTTGACCACCGCTCGCTTACTCCCGAGCGGCttttt                                            >  2:3467853/1‑93 (MQ=255)

                                             aTTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGTGTGCTGTCGGTTATTTGACCACCGCTCGCTTACTCCCGAGCGGCTTTTTa                                           <  2:4994282/93‑1 (MQ=255)

                                              ttACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGTGTGCTGTCGGTTATTTGACCACCGCTCGCTTACTCCCGAGCGGCTTTTTAt                                          <  1:59448/93‑1 (MQ=255)

                                               tACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGTGTGCTGTCGGTTATTTGACCACCGCTCGCTTACTCCCGAGCGGCTTTTTATc                                         >  2:3504537/1‑93 (MQ=255)

                                               tACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGTGTGCTGTCGGTTATTTGACCACCGCTCGCTTACTCCCGAGCGGCTTTTTATc                                         <  1:4604814/93‑1 (MQ=255)

                                                tcccGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGTGTGCTGTCGGTTATTTGACCACCGCTCGCTTACTCCCGAGCGGCTTTTTATCt                                        <  2:4728843/92‑1 (MQ=255)

                                                 cccGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGTGTGCTGTCGGTTATTTGACCACCGCTCGCTTACTCCCGAGCGGCTTTTTATCtt                                       >  2:1233517/1‑93 (MQ=255)

                                                 cccGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGTGTGCTGTCGGTTATTTGACCACCGCTCGCTTACTCCCGAGCGGCTTTTTATCtt                                       <  1:6029033/93‑1 (MQ=255)

                                                  ccGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGTGTGCTGTCGGTTATTTGACCACCGCTCGCTTACTCCCGAGCGGCTTTTTATCttt                                      >  1:2798155/1‑93 (MQ=255)

                                                    gcgcTTCGAGATTGCGGAGAGCTTTTTTAACTCCGTGTGCTGTCGGTTATTTGACCACCGCTCGCTTACTCCCGAGCGGCTTTTTATCTTTAg                                    <  1:4511761/93‑1 (MQ=255)

                                                    gcgcTTCGAGATTGCGGAGAGCTTTTTTAACTCCGTGTGCTGTCGGTTATTTGACCACCGCTCGCTTACTCCCGAGCGGCTTTTTATCTTTAg                                    >  2:5754831/1‑93 (MQ=255)

                                                    gcgcTTCGAGATTGCGGAGAGCTTTTTTAACTCCGTGTGCTGTCGGTTATTTGACCACCGCTCGCTTACTCCCGAGCGGCTTTTTATCTTTAg                                    <  2:4292559/93‑1 (MQ=255)

                                                    gcgcTTCGAGATTGCGGAGAGCTTTTTTAACTCCGTGTGCTGTCGGTTATTTGACCACCGCTCGCTTACTCCCGAGCGGCTTTTTATCTTTAg                                    <  2:4866931/93‑1 (MQ=255)

                                                       cTTCGAGATTGCGGAGAGCTTTTTTAACTCCGTGTGCTGTCGGTTATTTGACCACCGCTCGCTTACTCCCGAGCGGCTTTTTATCTTTAGctc                                 <  1:5788340/93‑1 (MQ=255)

                                                       cTTCGAGATTGCGGAGAGCTTTTTTAACTCCGTGTGCTGTCGGTTATTTGACCACCGCTCGCTTACTCCCGAGCGGCTTTTTATCTTTAGctc                                 <  2:1079847/93‑1 (MQ=255)

                                                       cTTCGAGATTGCGGAGAGCTTTTTTAACTCCGTGTGCTGTCGGTTATTTGACCACCGCTCGCTTACTCCCGAGCGGCTTTTTATCTTTAGctc                                 <  2:2692151/93‑1 (MQ=255)

                                                       cTTCGAGATTGCGGAGAGCTTTTTTAACTCCGTGTGCTGTCGGTTATTTGACCACCGCTCGCTTACTCCCGAGCGGCTTTTTATCTTTAGctc                                 <  1:2437302/93‑1 (MQ=255)

                                                        ttCGAGATTGCGGAGAGCTTTTTTAACTCCGTGTGCTGTCGGTTATTTGACCACCGCTCGCTTACTCCCGAGCGGCTTTTTATCTTTAGctct                                <  2:2222583/93‑1 (MQ=255)

                                                        ttCGAGATTGCGGAGAGCTTTTTTAACTCCGTGTGCTGTCGGTTATTTGACCACCGCTCGCTTACTCCCGAGCGGCTTTTTATCTTTAGctct                                >  2:3124761/1‑93 (MQ=255)

                                                        ttCGAGATTGCGGAGAGCTTTTTTAACTCCGTGTGCTGTCGGTTATTTGACCACCGCTCGCTTACTCCCGAGCGGCTTTTTATCTTTAGctct                                >  2:1854493/1‑93 (MQ=255)

                                                          cGAGATTGCGGAGAGCTTTTTTAACTCCGTGTGCTGTCGGTTATTTGACCACCGCTCGCTTACTCCCGAGCGGCTTTTTATCTTTAGCTCTCa                              <  1:4939367/93‑1 (MQ=255)

                                                           gagaTTGCGGAGAGCTTTTTTAACTCCGTGTGCTGTCGGTTATTTGACCACCGCTCGCTTACTCCCGAGCGGCTTTTTATCTTTAGCTCTCAg                             >  2:5926898/1‑93 (MQ=255)

                                                            agaTTGCGGAGAGCTTTTTTAACTCCGTGTGCTGTCGGTTATTTGACCACCGCTCGCTTACTCCCGAGCGGCTTTTTATCTTTAGCTCTCAGc                            >  1:639830/1‑93 (MQ=255)

                                                             gaTTGCGGAGAGCTTTTTTAACTCCGTGTGCTGTCGGTTATTTGACCACCGCTCGCTTACTCCCGAGCGGCTTTTTATCTTTAGCTCTCAGcc                           >  1:760879/1‑93 (MQ=255)

                                                             gaTTGCGGAGAGCTTTTTTAACTCCGTGTGCTGTCGGTTATTTGACCACCGCTCGCTTACTCCCGAGCGGCTTTTTATCTTTAGCTCTCAGcc                           >  1:331749/1‑93 (MQ=255)

                                                              aTTGCGGAGAGCTTTTTTAACTCCGTGTGCTGTCGGTTATTTGACCACCGCTCGCTTACTCCCGAGCGGCTTTTTATCTTTAGCTCTCAGCCa                          >  2:1652914/1‑93 (MQ=255)

                                                                 gCGGAGAGCTTTTTTAACTCCGTGTGCTGTCGGTTATTTGACCACCGCTCGCTTACTCCCGAGCGGCTTTTTATCTTTAGCTCTCAGCCagag                       <  2:3317638/93‑1 (MQ=255)

                                                                  gggAGAGCTTTTTTAACTCCGTGTGCTGTCGGTTATTTGACCACCGCTCGCTTACTCCCGAGCGGCTTTTTATCTTTAGCTCTCAGCCAGAgc                      <  2:4055397/92‑1 (MQ=255)

                                                                  cGGAGAGCTTTTTTAACTCCGTGTGCTGTCGGTTATTTGACCACCGCTCGCTTACTCCCGAGCGGCTTTTTATCTTTAGCTCTCAGCCAGAgc                      >  1:2856541/1‑93 (MQ=255)

                                                                   tgagaGCTTGTTTAACTCCGGGTGCGTTCGGTTATTTGACCACCGCTCGCTTACTCCCGAGCGGCTTTTTATCTTTAGCTCTCAGCCAGAgcg                     <  1:1420026/92‑1 (MQ=255)

                                                                    gagaGCTTTTTTAACTCCGTGTGCTGTCGGTTATTTGACCACCGCTCGCTTACTCCCGAGCGGCTTTTTATCTTTAGCTCTCAGCCAGAgcgc                    >  2:6267765/1‑93 (MQ=255)

                                                                    gagaGCTTTTTTAACTCCGTGTGCTGTCGGTTATTTGACCACCGCTCGCTTACTCCCGAGCGGCTTTTTATCTTTAGCTCTCAGCCAGAgcgc                    >  2:1927893/1‑93 (MQ=255)

                                                                     agagCTTTTTTAACTCCGTGTGCTGTCGGTTATTTGACCACCGCTCGCTTACTCCCGAGCGGCTTTTTATCTTTAGCTCTCAGCCAGAGcgcc                   <  1:3793994/93‑1 (MQ=255)

                                                                      gagCTTTTTTAACTCCGTGTGCTGTCGGTTATTTGACCACCGCTCGCTTACTCCCGAGCGGCTTTTTATCTTTAGCTCTCAGCCAGAGcgccg                  >  2:4409555/1‑93 (MQ=255)

                                                                        gCTTTTTTAACTCCGTGTGCTGTCGGTTATTTGACCACCGCTCGCTTACTCCCGAGCGGCTTTTTATCTTTAGCTCTCAGCCAGAGCGCCGCt                <  2:2388469/93‑1 (MQ=255)

                                                                         cTTTTTTAACTCCGTGTGCTGTCGGTTATTTGACAACCGCTCGCTTACTCCCGAGCGGCTTTTTATCTTTAGCTCTCAGCCAGAGCGCCGCtt               <  2:2885966/93‑1 (MQ=255)

                                                                            ttttACCTCCGTGTGCTGTCGGTTATTGGACCNCCNCTCGCTTACTCCCGAGCGGCTTTTTATCTTTAGCTCTCAGCCAGAGCGCCGCTTTCg            <  1:5103945/93‑1 (MQ=255)

                                                                            ttttAACTCCGTGTGCTGTCGGTTATTTGACCACCGCTCGCTTACTCCCGAGCGGCTTTTTATCTTTAGCTCTCAGCCAGAGCGCCGCTTTCg            <  2:2203257/93‑1 (MQ=255)

                                                                               tAACTCCGTGTGCTGTCGGTTATTTGACCACCGCTCGCTTACTCCCGAGCGGCTTTTTATCTTTAGCTCTCAGCCAGAGCGCCGCTTTCGTAc         >  1:2079822/1‑93 (MQ=255)

                                                                                aaCTCCGTGTGCTGTCGGTTATTTGACCACCGCTCGCTTACTCCCGAGCGGCTTTTTATTTTTAGCTCTCAGCCAGAGCGCCGCTTTCGTAcc        >  2:920758/1‑93 (MQ=255)

                                                                                aaCTCCGTGTGCTGTCGGTTATTTGACCACCGCTCGCTTACTCCCGAGCGGCTTTTTATCTTTAGCTCTCAGCCAGAGCGCCGCTTTCGTAcc        >  1:2505566/1‑93 (MQ=255)

                                                                                aaCTCCGTGTGCTGTCGGTTATTTGACCACCGCTCGCTTACTCCCGAGCGGCTTTTTATCTTTAGCTCTCAGCCAGAGCGCCGCTTTCGTAcc        <  1:2741995/93‑1 (MQ=255)

                                                                                 aCTCCGTGTGCTGTCGGTTATTTGACCACCGCTCGCTTACTCCCGAGCGGCTTTTTATCTTTAGCTCTCAGCCAGAGCGCCGCTTTCGTACCa       <  1:4837440/93‑1 (MQ=255)

                                                                                 aCTCCGTGTGCTGTCGGTTATTTGACCACCGCTCGCTTACTCCCGAGCGGCTTTTTATCTTTAGCTCTCAGCCAGAGCGCCGCTTTCGTACCa       >  2:5580277/1‑93 (MQ=255)

                                                                                 aCTCCGTGTGCTGTCGGTTATTTGACCACCGCTCGCTTACTCCCGAGCGGCTTTTTATCTTTAGCTCTCAGCCAGAGCGCCGCTTTCGTACCa       <  1:1501345/93‑1 (MQ=255)

                                                                                  cTCCGTGTGCTGTCGGTTATTTGACCACCGCTCGCTTACTCCCGAGCGGCTTTTTATCTTTAGCTCTCAGCCAGAGCGCCGCTTTCGTACCAt      <  1:6252152/93‑1 (MQ=255)

                                                                                   tCCGTGTGCTGTCGGTTATTTGACCACCGCTCGCTTACTCCCGAGCGGCTTTTTATCTTTAGCTCTCAGCCAGAGCGCCGCTTTCGTACCAtt     >  2:2618313/1‑93 (MQ=255)

                                                                                    ccGTGTGCTGTCGGTTATTTGACCACCGCTCGCTTACTCCCGAGCGGCTTTTTATCTTTAGCTCTCAGCCAGAGCGCCGCTTTCGTACCATTc    <  2:1411713/93‑1 (MQ=255)

                                                                                     cGTGTGCTGTCGGTTATTTGACCACCGCTCGCTTACTCCCGAGCGGCTTTTTATCTTTAGCTCTCAGCCAGAGCGCCGCTTTCGTACCATTcc   <  2:5292586/93‑1 (MQ=255)

                                                                                      gtgtGCTGTCGGTTATTTGACCACCGCTCGCTTACTCCCGAGCGGCTTTTTATCTTTAGCTCTCAGCCAGAGCGCCGCTTTCGTACCATTccc  >  2:1739147/1‑93 (MQ=255)

                                                                                      gtgtGCTGTCGGTTATTTGACCACCGCTCGCTTACTCCCGAGCGGCTTTTTATCTTTAGCTCTCAGCCAGAGCGCCGCTTTCGTACCATTccc  >  2:2357156/1‑93 (MQ=255)

CATTTTTACTACGTGTTAAAGAGCATTACACCCGGCTGTTGCCGGATTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGTGTACTGTCGGTTATTTGACCACCGCTCGCTTACTCCCGAGCGGCTTTTTATCTTTAGCTCTCAGCCAGAGCGCCGCTTTCGTACCATTCCC  >  NC_000913/4216404‑4216582

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 20 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`