BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,244,543	T→C	F61L (TTC→CTC)	malK →	ATP‑binding component of transport system for maltose

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,244,543	0	T	C	100.0%	746.3 / NA	227	F61L (TTC→CTC)	malK	ATP‑binding component of transport system for maltose
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (94/133); total (94/133)

Only 100 of 235 total aligned reads displayed.

TTCGTGGTGTTTGTCGGACCGTCTGGCTGCGGTAAATCGACTTTACTGCGCATGATTGCCGGGCTTGAGACGATCACCAGCGGCGACCTGTTCATCGGTGAGAAACGGATGAATGACACTCCGCCAGCAGAACGCGGCGTTGGTATGGTGTTTCAGTCTTACGCGCTCTATCCCCACCTG  >  NC_000913/4244453‑4244632

ttCGTGGTGTTTGTCGGACCGTCTGGCTGCGGTAAATCGACTTTACTGCGCATGATTGCCGGGCTTGAGACGATCACCAGCGGCGCCCTGCTc                                                                                         >  2:1529666/1‑93 (MQ=255)

 tCGTGGTGTTTGTCGGACCGTCTGGCTGCGGTAAATCGACTTTACTGCGCATGATTGCCGGGCTTGAGACGATCACCAGCGGCGACCTGCTCa                                                                                        <  1:1245358/93‑1 (MQ=255)

 tCGTGGTGTTTGTCGGACCGTCTGGCTGCGGTAAATCGACTTTACTGCGCATGATTGCCGGGCTTGAGACGATCACCAGCGGCGACCTGCTCa                                                                                        <  1:4318928/93‑1 (MQ=255)

    tggtgTTTGTCGGACCGTCTGGCTGCGGTAAATCGACTTTACTGCGCATGATTGCCGGGCTTGAGACGATCACCAGCGGCGACCTGCTCATCg                                                                                     >  1:990579/1‑93 (MQ=255)

     ggtgTTTGTCGGACCGTCTGGCTGCGGTAAATCGACTTTACTGCGCATGATTGCCGGGCTTGAGACGATCACCAGCGGCGACCTGCTCATCgg                                                                                    <  2:2094174/93‑1 (MQ=255)

      gtgtTTGTCGGACCGTCTGGCTGCGGTAAATCGACTTTACTGCGCATGATTGCCGGGCTTGAGACGATCACCAGCGGCGACCTGCTCATCGGt                                                                                   <  1:2190013/93‑1 (MQ=255)

       tgtTTGTCGGACCGTCTGGCTGCGGTAAATCGACTTTACTGCGCATGATTGCCGGGCTTGAGACTATCACCAGCGGCGACCTGCTCATCGGTg                                                                                  >  2:6447470/1‑93 (MQ=255)

       tgtTTGTCGGACCGTCTGGCTGCGGTAAATCGACTTTACTGCGCATGATTGCCGGGCTTGAGACGATCACCAGCGGCGACCTGCTCATCGGTg                                                                                  <  1:3053650/93‑1 (MQ=255)

        gtTTGTCGGACCGTCTGGCTGCGGTAAATCGACTTTACTGCGCATGATTGCCGGGCTTGAGACGATCACCAGCGGCGACCTGCTCATCGGTga                                                                                 <  1:3457103/93‑1 (MQ=255)

        gtTTGTCGGACCGTCTGGCTGCGGTAAATCGACTTTACTGCGCATGATTGCCGGGCTTGAGACGATCACCAGCGGCGACCTGCTCATCGGTga                                                                                 >  2:4629659/1‑93 (MQ=255)

        gtTTGTCGGACCGTCTGGCTGCGGTAAATCGACTTTACTGCGCATGATTGCCGGGCTTGAGACGATCACCAGCGGCGACCTGCTCATCGGTga                                                                                 >  1:2979715/1‑93 (MQ=255)

        gtTTGTCGGACCGTCTGGCTGCGGTAAATCGACTTTACTGCGCATGATTGCCGGGCTTGAGACGATCACCAGCGGCGACCTGCTCATCGGTga                                                                                 <  1:1764793/93‑1 (MQ=255)

        gtTTGTCGGACCGTCTGGCTGCGGTAAATCGACTTTACTGCGCATGATTGCCGGGCTTGAGACGATCACCAGCGGCGACCTGCTCATCGGTga                                                                                 <  2:1014728/93‑1 (MQ=255)

        gtTTGTCGGACCGTCTGGCTGCGGTAAATCGACTTTACTGCGCATGATTGCCGGGCTTGAGACGATCACCAGCGGCGACCTGCTCATCGGTga                                                                                 <  2:129009/93‑1 (MQ=255)

          ttGTCGGACCGTCTGGCTGCGGTAAATCGACTTTACTGCGCATGATTGCCGGGCTTGAGACGATCACCAGCGGCGACCTGCTCATCGGTgaga                                                                               >  2:2561193/1‑93 (MQ=255)

            gTCGGACCGTCTGGCTGCGGTAAATCGACTTTACTGCGCATGATTGCCGGGCTTGAGACGATCACCAGCGGCGACCTGCTCATCGGTGAGaaa                                                                             >  1:574606/1‑93 (MQ=255)

            gTCGGACCGTCTGGCTGCGGTAAATCGACTTTACTGCGCATGATTGCCGGGCTTGAGACGATCACCAGCGGCGACCTGCTCATCGGTGAGaaa                                                                             <  2:1501426/93‑1 (MQ=255)

            gTCGGACCGTCTGGCTGCGGTAAATCGACTTTACTGCGCATGATTGCCGGGCTTGAGACGATCACCAGCGGCGACCTGCTCATCGGTGAGaaa                                                                             <  2:6694372/93‑1 (MQ=255)

               ggACCGTCTGGCTGCGGTAAATCGACTTTACTGCGCATGATTGCCGGGCTTGAGACGATCACCAGCGGCGACCTGCTCATCGGTGAGAAACgg                                                                          >  2:1817896/1‑93 (MQ=255)

                   cGTCTGGCTGCGGTAAATCGACTTTACTGCGCATGATTGCCGGGCTTGAGACGATCACCAGCGGTGAACTGCTCATCGGTGAGGAACGGatga                                                                      >  1:3344297/1‑93 (MQ=37)

                    gTCTGGCTGCGGTAAATCGACTTTACTGCGCATGATTGCCGGGCTTGAGACGATCACCAGCTGCGACCTGCTCATCGGTGAGAAACGGatgaa                                                                     >  1:1493255/1‑93 (MQ=255)

                    gTCTGGCTGCGGTAAATCGACTTTACTGCGCATGATTGCCGGGCTTGAGACGATCACCAGCGGCGACCTGCTCATCGGTGAGAAACGGatgaa                                                                     >  1:5798225/1‑93 (MQ=255)

                    gTCTGGCTGCGGTAAATCGACTTTACTGCGCATGATTGCCGGGCTTGAGACGATCACCAGCGGCGACCTGCTCATCGGTGAGAAACGGatgaa                                                                     >  1:2345512/1‑93 (MQ=255)

                     tCTGGCTGCGGTAAATCGACTTTACTGCGCATGATTGCCGGGCTTGAGACGATCACCAGCGGCGACCTGCTCATCGGTGAGAAACGGatgaat                                                                    <  1:865711/93‑1 (MQ=255)

                     tCTGGCTGCGGTAAATCGACTTTACTGCGCATGATTGCCGGGCTTGAGACGATCACCAGCGGCGACCTGCTCATCGGTGAGAAACGGatgaat                                                                    >  2:426306/1‑93 (MQ=255)

                       tGGCTGCGGTAAATCGACTTTACTGCGCATGATTGCCGGGCTTGAGACGATCACCAGCGGCGACCTGCTCATCGGTGAGAAACGGatgaatga                                                                  <  2:3260376/93‑1 (MQ=255)

                        ggCTGCGGTAAATCGACTTTACTGCGCATGATTGCCGGGCTTGAGACGATCACCAGCGGCGACCTGCTCATCGGTGAGAAACGGATGAATGac                                                                 >  1:2460557/1‑93 (MQ=255)

                          cTGCGGTAAATCGACTTTACTGCGCATGATTGCCGGGCTTGAGACGATCACCAGCGGCGACCTGCTCATCGGTGAGAAACGGATGAATGacac                                                               <  2:4076823/93‑1 (MQ=255)

                          cTGCGGTAAATCGACTTTACTGCGCATGATTGCCGGGCTTGAGACGATCACCAGCGGCGACCTGCTCATCGGTGAGAAACGGATGAATGacac                                                               <  2:3814060/93‑1 (MQ=255)

                          cTGCGGTAAATCGACTTTACTGCGCATGATTGCCGGGCTTGAGACGATCACCAGCGGCGACCTGCTCATCGGTGAGAAACGGATGAATGacac                                                               <  2:1907824/93‑1 (MQ=255)

                            gCGGTAAATCGACTTTACTGCGCATGATTGCCGGGCTTGAGACGATCACCAGCGGCGACCTGCTCATCGGTGAGAAACGGATGAATGACACTc                                                             <  1:3288435/93‑1 (MQ=255)

                            gCGGTAAATCGACTTTACTGCGCATGATTGCCGGGCTTGAGACGATCACCAGCGGCGACCTGCTCATCGGTGAGAAACGGATGAATGACACTc                                                             <  2:1550016/93‑1 (MQ=255)

                             cGGTAAATCGACTTTACTGCGCATGATTGCCGGGCTTGAGACGATCACCAGCGGCGACCTGCTCATCGGTGAGAAACGGATGAATGACACTcc                                                            <  1:4495509/93‑1 (MQ=255)

                               gTAAATCGACTTTACTGCGCATGATTGCCGGGCTTGAGACGATCACCAGCGGCGACCTGCTCATCGGTGAGAAACGGATGAATGACACTCCGc                                                          >  2:5525734/1‑93 (MQ=255)

                               gTAAATCGACTTTACTGCGCATGATTGCCGGGCTTGAGACGATCACCAGCGGCGACCTGCTCATCGGTGAGAAACGGATGAATGACACTCCGc                                                          >  1:6452037/1‑93 (MQ=255)

                               gTAAATCGACTTTACTGCGCATGATTGCCGGGCTTGAGACGATCACCAGCGGCGACCTGCTCATCGGTGAGAAACGGATGAATGACACTCCGc                                                          <  2:5417526/93‑1 (MQ=255)

                                  aaTCGACTTTACTGCGCATGATTGCCGGGCTTGAGACGATCACCAGCGGCGACCTGCTCATCGGTGAGAAACGGATGAATGACACTCCGCcag                                                       >  2:4794230/1‑93 (MQ=255)

                                    tCGACTTTACTGCGCATGATTGCCGGGCTTGAGACGATCACCAGCGGCGACCTGCTCATCGGTGAGAAACGGATGAATGACACTCCGCcagca                                                     <  2:2112717/93‑1 (MQ=255)

                                       aCTTTACTGCGCATGATTGCCGGGCTTGAGACGATCACCAGCGGCGACCTGCTCATCGGTGAGAAACGGATGAATGACACTCCGCCAGCAGaa                                                  <  1:1854532/93‑1 (MQ=255)

                                       aCTTTACTGCGCATGATTGCCGGGCTTGAGACGATCACCAGCGGCGACCTGCTCATCGGTGAGAAACGGATGAATGACACTCCGCCAGCAGaa                                                  <  2:2989360/93‑1 (MQ=255)

                                        cTTTACTGCGCATGATTGCCGGGCTTGAGACGATCACCAGCGGCGACCTGCTCATCGGTGAGAAACGGATGAATGACACTCCGCCAGCAGAAc                                                 <  2:3603466/93‑1 (MQ=255)

                                        cTTTACTGCGCATGATTGCCGGGCTTGAGACGATCACCAGCGGCGACCTGCTCATCGGTGAGAAACGGATGAATGACACTCCGCCAGCAGAAc                                                 >  2:3636247/1‑93 (MQ=255)

                                          ttACTGCGCATGATTGCCGGGCTTGAGACGATCACCAGCGGCGACCTGCTCATCGGTGAGAAACGGATGAATGACACTCCGCCAGCAGAAcgc                                               <  2:5660552/93‑1 (MQ=255)

                                           tACTGCGCATGATTGCCGGGCTTGAGACGATCACCAGCGGCGACCTGCTCATCGGTGAGAAACGGATGAATGACACTCCGCCAGCAGCAcgcg                                              >  1:5979366/1‑93 (MQ=255)

                                           tACTGCGCATGATTGCCGGGCTTGAGACGATCACCAGCGGCGACCTGCTCATCGGTGAGAAACGGATGAATGACACTCCGCCAGCAGAAcgcg                                              <  1:5212695/93‑1 (MQ=255)

                                              tGCGCATGATTGCCGGGCTTGAGACGATCACCAGCGGCGACCTGCTCATCGGTGAGAAACGGATGAATGACACTCCGCCAGCAGAACgcggcg                                           >  2:5108864/1‑93 (MQ=255)

                                               gcgcATGATTGCCGGGCTTGAGACGATCACCAGCGGCGACCTGCTCATCGGTGAGAAACGGATGAATGACACTCCGCCAGCAGAACGCGGCGt                                          <  2:5336252/93‑1 (MQ=255)

                                               gcgcATGATTGCCGGGCTTGAGACGATCACCAGCGGCGACCTGCTCATCGGTGAGAAACGGATGAATGACACTCCGCCAGCAGAACGCGGCGt                                          <  2:801963/93‑1 (MQ=255)

                                               gcgcATGATTGCCGGGCTTGAGACGATCACCAGCGGCGACCTGCTCATCGGTGAGAAACGGATGAATGACACTCCGCCAGCAGAACGCGGCGt                                          <  1:1129327/93‑1 (MQ=255)

                                               gcgcATGATTGCCGGGCTTGAGACGATCACCAGCGGCGACCTGCTCATCGGTGAGAAACGGATGAATGACACTCCGCCAGCAGAACGCGGCGt                                          <  1:2301473/93‑1 (MQ=255)

                                                cgcATGATTGCCGGGCTTGAGACGATCACCAGCGGCGACCTGCTCATCGGTGAGAAACGGATGAATGACACTCCGCCAGCAGAACGCGGCGtt                                         <  1:3557619/93‑1 (MQ=255)

                                                 gcATGATTGCCGGGCTTGAGACGATCACCAGCGGCGACCTGCTCATCGGTGAGAAACGGATGAATGACACTCCGCCAGCAGAACGCGGCGTTg                                        >  2:1210585/1‑93 (MQ=255)

                                                 gcATGATTGCCGGGCTTGAGACGATCACCAGCGGCGACCTGCTCATCGGTGAGAAACGGATGAATGACACTCCGCCAGCAGAACGCGGCGTTg                                        >  1:1678079/1‑93 (MQ=255)

                                                 gcATGATTGCCGGGCTTGAGACGATCACCAGCGGCGACCTGCTCATCGGTGAGAAACGGATGAATGACACTCCGCCAGCAGAACGCGGCGTTg                                        >  2:4779472/1‑93 (MQ=255)

                                                    tGATTGCCGGCCTTGAGACGATCACCAGCGGCGACCTGCTCATCGGTGAGAAACGGATGAATGACACTCCGCCAGCAGAACGCGGCGTTGGTa                                     <  1:3267585/93‑1 (MQ=255)

                                                     gATTGCCGGGCTTGAGACGATCACCAGCGGCGACCTGCTCATCGGTGAGAAACGGATGAATGACACTCCGCCAGCAGAACGCGGCGTTGGTAt                                    >  2:1342467/1‑93 (MQ=255)

                                                      aTTGCCGGGCTTGAGACGATCACCAGCGGCGACCTGCTCATCGGTGAGAAACGGATGAATGACACTCCGCCAGCAGAACGCGGCGTTGGTATg                                   <  1:473876/93‑1 (MQ=255)

                                                       ttGCCGGGCTTGAGACGATCACCAGCGGCGACCTGCTCATCGATGAGAAACGGATGAATGACACTCCGCCAGCAGAACGCGGCGTTGGTATgg                                  >  2:3572573/1‑93 (MQ=255)

                                                        tGCCGGGCTTGAGACGATCACCAGCGGCGACCTGCTCATCGGTGAGAAACGGATGAATGACACTCCGCCAGCAGAACGCGGCGTTGGTATGgt                                 <  2:3350095/93‑1 (MQ=255)

                                                          ccGGGCTTGAGACGATCACCAGCGGCGACCTGCTCATCGGTGAGAAACGGATGAATGACACTCCGCCAGCAGAACGCGGCGTTGGTATGgtgt                               <  2:852420/93‑1 (MQ=255)

                                                           cGGGCTTGAGACGATCACCAGCGGCGACCTGCTCATCGGTGAGAAACGGATGAATGACACTCCGCCAGCAGAACGCGGCGTTGGTATGGTGtt                              <  2:6005855/93‑1 (MQ=255)

                                                            gggCTTGAGACGATCACCAGCGGCGACCTGCTCATCGGTGAGAAACGGATGAATGACACTCCGCCAGCAGAACGCGGCGTTGGTATGGTGttt                             >  1:6671324/1‑93 (MQ=255)

                                                            gggCTTGAGACGATCACCAGCGGCGACCTGCTCATCGGTGAGAAACGGATGAATGACACTCCGCCAGCAGAACGCGGCGTTGGTATGGTGttt                             <  1:5919036/93‑1 (MQ=255)

                                                            gggCTTGAGACGATCACCAGCGGCGACCTGCTCATCGGTGAGAAACGGATGAATGACACTCCGCCAGCAGAACGCGGCGTTGGTATGGTGttt                             >  2:5579263/1‑93 (MQ=255)

                                                             ggCTTGAGACGATCACCAGCGGCGACCTGCTCATCGGTGAGAAACGGATGAATGACACTCCGCCAGCAGAACGCGGCGTTGGTATGGTGTTTc                            >  2:6714869/1‑93 (MQ=255)

                                                             ggCTTGAGACGATCACCAGCGGCGACCTGCTCATCGGTGAGAAACGGATGAATGACACTCCGCCAGCAGAACGCGGCGTTGGTATGGTGTTTc                            >  1:1966569/1‑93 (MQ=255)

                                                               cTTGAGACGAGCACCAGCGGCGACCTGCTCATCGGTGAGAAACGGATGAATGACACTCCGCCAGCAGAACGCGGCTTTGGTATGGTGTTTCAg                          <  2:3442755/93‑1 (MQ=255)

                                                                 tGAGACGATCACCAGCGGCGACCTGCTCATCGGTGAGAAACGGATGAATGACACTCCGCCAGCAGAACGCGGCGTTGGTATGGTGTTTCAGTc                        <  2:695179/93‑1 (MQ=255)

                                                                  gagaCGATCACCAGCGGCGACCTGCTCATCGGTGAGAAACGGATGAATGACACTCCGCCAGCAGAACGCGGCGTTGGTATGGTGTTTCAGTCt                       <  2:2557152/93‑1 (MQ=255)

                                                                  gagaCGATCACCAGCGGCGACCTGCTCATCGGTGAGAAACGGATGAATGACACTCCGCCAGCAGAACGCGGCGTTGGTATGGTGTTTCAGTCt                       <  2:1721405/93‑1 (MQ=255)

                                                                   agaCGATCACCAGCGGCGACCTGCTCATCGGTGAGAAACGGATGAATGACACTCCGCCAGCAGAACGCGGCGTTGGTATGGTGTTTCAGTCtt                      <  2:6190254/93‑1 (MQ=255)

                                                                   agaCGATCACCAGCGGCGACCTGCTCATCGGTGAGAAACGGATGAATGACACTCCGCCAGCAGAACGCGGCGTTGGTATGGTGTTTCAGTCtt                      <  1:1379775/93‑1 (MQ=255)

                                                                    gaCGATCACCAGCGGCGACCTGCTCATCGGTGAGAAACGGATGAATGACACTCCGCCAGCAGAACGCGGCGTTGGTATGGTGTTTCAGTCTTa                     <  1:5153955/93‑1 (MQ=255)

                                                                     gcGATCGCCAGCGACGCCCTGCTCATCGGTGAGAAACGGATCAATGACACTCCGCCAGCAGAACGCGGCGTTGGTATGGTGTTTCAGTCGTAc                    <  1:647701/92‑1 (MQ=255)

                                                                     aCGATCACCAGCGGCGACCTGCTCATCGGTGAGAAACGGATGAATGACACTCCGCCAGCAGAACGCGGCGTTGGTATGGTGTTTCAGTCTTAc                    <  1:6243545/93‑1 (MQ=255)

                                                                     aCGATCACCAGCGGCGACCTGCTCATCGGTGAGAAACGGATGAATGACACTCCGCCAGCAGAACGCGGCGTTGGTATGGTGTTTCAGTCTTAc                    <  1:3892860/93‑1 (MQ=255)

                                                                     aCGATCACCAGCGGCGACCTGCTCATCGGTGAGAAACGGATGAATGACACTCCGCCAGCAGAACGCGGCGTTGGGATGGTGTTTCAGTCTTAc                    >  2:5873414/1‑93 (MQ=255)

                                                                      cGATCACCAGCGGCGACCTGCTCATCGGTGAGAAACGGATGAATGACACTCCGCCAGCAGAACGCGGCGTTGGTATGGTGTTTCAGTCTTAcg                   <  2:5606798/93‑1 (MQ=255)

                                                                      cGATCACCAGCGGCGACCTGCTCATCGGTGAGAAACGGATGAATGACACTCCGCCAGCAGAACGCGGCGTTGGTATGGTGTTTCAGTCTTAcg                   <  2:1730934/93‑1 (MQ=255)

                                                                         tCACCAGCGGCGACCTGCTCATCGGTGAGAAACGGATGAATGACACTCCGCCAGCAGAACGCGGCGTTGGTATGGTGTTTCAGTCTTACgcgc                >  2:4391866/1‑93 (MQ=255)

                                                                           aCCAGCGGCGACCTGCTCATCGGTGAGAAACGGATGAATGACACTCCGCCAGCAGAACGCGGCGTTGGTATGGTGTTTCAGTCTTACGCGctc              <  2:5962942/93‑1 (MQ=255)

                                                                            ccAGCGGCGAACTGCTCATCGGTGAGAAACGGATGAATGACACTCCGCCAGCAGAACGCGGCGTTGGTATGGTGTTTCAGTCTTACGCGctct             >  1:4074756/1‑93 (MQ=255)

                                                                               gcggcgACCTGCTCATCGGTGAGAAACGGATGAATGACACTCCGCCAGCAGAACGCGGCGTTGGTATGGTGTTTCAGTCTTACGCGCTCTATc          >  1:1044170/1‑93 (MQ=255)

                                                                               gcggcgACCTGCTCATCGGTGAGAAACGGATGAATGACACTCCGCCAGCAGAACGCGGCGTTGGTATGGTGTTTCAGTCTTACGCGCTCTATc          >  2:5165951/1‑93 (MQ=255)

                                                                               gcggcgACCTGCTCATCGGTGAGAAACGGATGAATGACACTCCGCCAGCAGAACGCGGCGTTGGTATGGTGTTCCAGTCTTACGCGCTCTATc          >  1:699904/1‑93 (MQ=255)

                                                                                cggcgACCTGCTCATCGGTGAGAAACGGATGAATGACACTCCGCCAGCAGAACGCGGCGTTGGTATGGTGTTTCAGTCTTACGCGCTCTATcc         <  1:3777145/93‑1 (MQ=255)

                                                                                 ggcgACCTGCTCATCGGTGAGAAACTGATGAATGACACTCCGCCAGCAGAACGCGGCGTTGGTATGGTGTTTCAGTCTTACGCGCTCTATccc        <  2:6480048/93‑1 (MQ=255)

                                                                                  gcgACCTGCTCATCGGTGAGAAACGGATGAATGACGCTCCGCCAGCAGAACGCGGCGTTGGTATGGTGTTTCAGTCTTACGCGCTCTATcccc       <  1:4142803/93‑1 (MQ=255)

                                                                                  gcgACCTGCTCATCGGTGAGAAACGGATGAATGACACTCCGGCAGCAGAACGCGGCGTTGGTATGGTGTTTCAGTCTTACGCGCTCTATcccc       <  1:5849217/93‑1 (MQ=255)

                                                                                  gcgACCTGCTCATCGGTGAGAAACGGATGAATGACACTCCGCCAGCAGAACGCGGCGTTGGTATGGTGTTTCAGTCTTACGCGCTCTATcccc       >  2:2789143/1‑93 (MQ=255)

                                                                                     aCCTGCTCATCGGTGAGAAACGGATGAATGACACTCCGCCAGCAGAACGCGGCGTTGGTATGGTGTTTCAGTCTTACGCGCTCTATCCCCAcc    >  2:2259727/1‑93 (MQ=255)

                                                                                     aCCTGCTCATCGGTGAGAAACGGATGAATGACACTCCGCCAGCAGAACGCGGCGTTGGTATGGTGTTTCAGTCTTACGCGCTCTATCCCCAcc    <  2:2497096/93‑1 (MQ=255)

                                                                                     aCCTGCTCATCGGTGAGAAACGGATGAATGACACTCCGCCAGCAGAACGCGGCGTTGGTATGGTGTTTCAGTCGTACGCGCTCTATCCCCAcc    <  1:6539864/93‑1 (MQ=255)

                                                                                     aCCTGCTCATCGGTGACAAACGGATGAATGACACTCCGCCAGCAGAACGCGGCGTTGGTATGGTGTTTCAGTCTTACGCGCTCTATCCCCAcc    <  1:4454044/93‑1 (MQ=255)

                                                                                      ccTGCTCATCGGTGAGAAACGGATGAATGACACTCCGCCAGCAGAACGCGGCGTTGGTATGGTGTTTCAGTCTTACGCGCTCTATCCCCACCt   <  2:4991440/93‑1 (MQ=255)

                                                                                      ccTGCTCATCGGTGAGAAACGGATGAATGACACTCCGCCAGCAGAACGCGGCGTTGGTATGGTGTTTCAGTCTTACGCGCTCTATCCCCACCt   <  2:4524021/93‑1 (MQ=255)

                                                                                      ccTGCTCATCGGTGAGAAACGGATGAATGACACTCCGCCAGCAGAACGCGGCGTTGGTATGGTGTTTCAGTCTTACGCGCTCTATCCCCACCt   <  2:3105133/93‑1 (MQ=255)

                                                                                       cTGCTCATCGGTGAGAAACGGATGAATGACACTCCGCCAGCAGAACGCGGCGTTGGTATGGTGTTTCAGTCTTACGCGCTCTATCCCCACCTg  <  2:1980126/93‑1 (MQ=255)

                                                                                       cTGCTCATCGGTGAGAAACGGATGAATGACACTCCGCCAGCAGAACGCGGCGTTGGTATGGTGTTTCAGTCTTACGCGCTCTATCCCCACCTg  <  1:4372478/93‑1 (MQ=255)

                                                                                       cTGCTCATCGGTGAGAAACGGATGAATGACACTCCGCCAGCAGAACGCGGCGTTGGTATGGTGTTTCAGTCTTACGCGCTCTATCCCCACCTg  >  1:5489763/1‑93 (MQ=255)

TTCGTGGTGTTTGTCGGACCGTCTGGCTGCGGTAAATCGACTTTACTGCGCATGATTGCCGGGCTTGAGACGATCACCAGCGGCGACCTGTTCATCGGTGAGAAACGGATGAATGACACTCCGCCAGCAGAACGCGGCGTTGGTATGGTGTTTCAGTCTTACGCGCTCTATCCCCACCTG  >  NC_000913/4244453‑4244632

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 20 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`