BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,302,652	A→G	Y513H (TAT→CAT)	yjcS ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,302,652	0	A	G	99.6%	917.0 / ‑4.7	282	Y513H (TAT→CAT)	yjcS	hypothetical protein
Reads supporting (aligned to +/- strand): ref base A (0/1); new base G (134/146); total (135/147)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.66e-01

Only 100 of 293 total aligned reads displayed.

TGGTGCCGTGGCTGAACTTATGCACCCCTTCGCGCAGCTCTTTCGCGCCGGTCAGGTAGAAACCGCGCCATGTGGCGGACTCGGCCTGATAGCCCAGCTGTTCAAAGTTATTCGCTTGCAGATTCTTCGCGACCTGGTCACCCGGGTTGGCGGCAATCACCTGTTTCAGCAGTTCTGCCG  >  NC_000913/4302562‑4302741

tggtgCCGTGGCTGAACTTATGCACCCCTTCGCGCAGCTCTTTCGCGCCGGTCAGGTAGAAACCGCGCCATGTGGCGGACTCGGCCTGATGGc                                                                                         <  1:1861128/93‑1 (MQ=255)

     ccGTGGCTGAACTTATGCACCCCTTCGCGCAGCTCTTTCGCGCCGGTCAGGTAGAAACCGCGCCATGTGGCGGACTCGGCCTGATGGCCCAGc                                                                                    <  1:5888172/93‑1 (MQ=255)

     ccGTGGCTGAACTTATGCACCCCTTCGCGCAGCTCTTTCGCGCCGGTCAGGTAGAAACCGCGCCATGTGGCGGACTCGGCCTGATGGCCCAGc                                                                                    >  2:263559/1‑93 (MQ=255)

      cGTGGCTGAACTTATGCACCCCTTCGCGCAGCTCTTTCGCGCCGGTCAGGTAGAAACCGCGCCATGTGGCGGACTCGGCCTGATGGCCCAGCt                                                                                   <  2:4556090/93‑1 (MQ=255)

       gTGGCTGAACTTATGCACCCCTTCGCGCAGCTCTTTCGCGCCGGTCAGGTAGAAACCGCGCCATGTGGCGGACTCGGCCTGATGGGCCAGCTg                                                                                  >  2:6157747/1‑93 (MQ=255)

       gTGGCTGAACTTATGCACCCCTTCGCGCAGCTCTTTCGCGCCGGTCAGGTAGAAACCGCGCCATGTGGCGGACTCGGCCTGATGGCCCAGCTg                                                                                  >  1:6640234/1‑93 (MQ=255)

        tGGCTGAACTTATGCACCCCTTCGCGCAGCTCTTTCGCGCTGGTCAGGTAGAAACCGCGCCATGTGGCGGACTCGGCCTGATGGCCCAGCTGt                                                                                 <  2:1247511/93‑1 (MQ=255)

        tGGCTGAACTTATGCACCCCTTCGCGCAGCTCTTTCGCGCCGGTCAGGTAGAAACCGCGCCATGTGGCGGACTCGGCCTGATGGCCCCGCTGt                                                                                 >  1:4634598/1‑93 (MQ=255)

        tGGCTGAACTTATGCACCCCTTCGCGCAGCTCTTTCGCGCCGGTCAGGTAGAAACCGCGCCATGTGGCGGACTCGGCCTGATGGCCCAGCTGt                                                                                 <  1:3202564/93‑1 (MQ=255)

        tGGCTGAACTTATGCACCCCTTCGCGCAGCTCTGTCGCGCCGGTCAGGTAGAAACCGCGCCATGTGGCGGACTCGGCCTGATGGCCCAGCTGt                                                                                 <  2:227809/93‑1 (MQ=255)

        tGGCTGAACTTATGCACCCCTTCGCGCAGCTCTGTCGCGCCGGTCAGGTAGAAACCGCGCCATGTGGCGGACTCGGCCTGATGGCCCAGCTGt                                                                                 <  1:1259838/93‑1 (MQ=255)

         ggCTGAACTTATGCACCCCTTCGCGCAGCTCTTTCGCGCCGGTCAGGTAGAAACCGCGCCATGTGGCGGACTCGGCCTGATGGCCCCGCTGtt                                                                                >  1:2631861/1‑93 (MQ=255)

         ggCTGAACTTATGCACCCCTTCGCGCAGCTCTTTCGCGCCGGTCAGGTAGAAACCGCGCCATGTGGCGGACTCGGCCTGATGGCCCAGCTGtt                                                                                >  1:704682/1‑93 (MQ=255)

         ggCTGAACTTATGCACCCCTTCGCGCAGCTCTTTCGCGCCGGTCAGGTAGAAACCGCGCCATGTGGCGGACTCGGCCTGATGGCCCAGCTGtt                                                                                >  2:4951132/1‑93 (MQ=255)

           cTGAACTTATGCACCCCTTCGCGCAGCTCTTTCGCGCCGGTCAGGTAGAAACCGCGCCATGTGGCGGACTCGGCCTGATGGCCCAGCTGTTCa                                                                              <  2:5551878/93‑1 (MQ=255)

             gAACTTATGCACCCCTTCGCGCAGCTCTTTCGCGCCGGTCAGGTAGAAACCGCGCCATGTGGCGGACTCGGCCTGATGGCCCAGCTGTTCaaa                                                                            <  1:2858972/93‑1 (MQ=255)

                 ttATGCACCCCTTCGCGCAGCTCTTTCGCGCCGGTCAGGTAGAAACCGCGCCATGTGGCGGACTCGGCCTGATGGCCCAGCTGTTCAAAGTTa                                                                        >  2:1460840/1‑93 (MQ=255)

                 ttATGCACCCCTTCGCGCAGCTCTTTCGCGCCGGTCAGGTAGAAACCGCGCCATGTGGCGGACTCGGCCTGATGGCCCAGCTGTTCAAAGTTa                                                                        >  2:2379035/1‑93 (MQ=255)

                 ttATGCACCCCTTCGCGCAGCTCTTTCGCGCCGGTCAGGTAGAAACCGCGCCATGTGGCGGACTCGGCCTGATGGCCCAGCTGTTCAAAGTTa                                                                        <  1:5407601/93‑1 (MQ=255)

                     gCACCCCTTCGCGCAGCTCTTTCGCGCCGGTCAGGTAGAAACCGCGCCATGTGGCGGACTCGGCCTGATGGCCCAGCTGTTCAAAGTTATTcc                                                                    >  1:5217530/1‑92 (MQ=255)

                       aCCCCTTCGCGCAGCTCTTTCGCGCCGGTCAGGTAGAAACCGCGCCATGTGGCGGACTCGGCCTGATGGCCCAGCTGTTCAAAGTTATTCGCt                                                                  >  1:1024922/1‑93 (MQ=255)

                       aCCCCTTCGCGCAGCTCTTTCGCGCCGGTCAGGTAGAAACCGCGCCATGTGGCGGACTCGGCCTGATGGCCCAGCTGTTCAAAGTTATTCGCt                                                                  >  2:4148488/1‑93 (MQ=255)

                         cccTTCGCGCAGCTCTTTCGCGCCGGTCAGGTAGAAACCGCGCCATGTGGCGGACTCGGCCTGATGGCCCAGCTGTTCAAAGTTATTCGCTTg                                                                <  2:5422598/93‑1 (MQ=255)

                         cccTTCGCGCAGCTCTTTCGCGCCGGTCAGGTAGAAACCGCGCCATGTGGCGGACTCGGCCTGATGGCCCAGCTGTTCAAAGTTATTCGCTTg                                                                <  1:3294115/93‑1 (MQ=255)

                         cccTTCGCGCAGCTATTTCGCGCCGGTCAGGTAGAAACCGCGCCATGTGGCGGACTCGGCCTGATGGCCCAGCTGTTCAAAGGTATTCGCTTg                                                                >  1:1553898/1‑93 (MQ=255)

                          ccTTCGCGCAGCTCTTTCGCGCCGGTCAGGTAGAAACCGCGCCATGTGGCGGACTCGGCCTGATGGCCCAGCTGTTCAAAGTTATTCGCTTGc                                                               >  1:3541589/1‑93 (MQ=255)

                          ccTTCGCGCAGCTCTTTCGCGCCGGTCAGGTAGAAACCGCGCCATGTGGCGGACTCGGCCTGATGGCCCAGCTGTTCAAAGTTATTCGCTTGc                                                               <  1:3740008/93‑1 (MQ=255)

                          ccTTCGCGCAGCTCTTTCGCGCCGGTCAGGTAGAAACCGCGCCATGTGGCGGACTCGGCCTGATGGCCCAGCTGTTCAAAGTTATTCGCTTGc                                                               >  1:5679994/1‑93 (MQ=255)

                          ccTTCGCGCAGCTCTTTCGCGCCGGTCAGGTAGAAACCGCGCCATGTGGCGGACTCGGCCTGATGGCCCAGCTGTTCAAAGTTATTCGCTTGc                                                               <  2:4267539/93‑1 (MQ=255)

                          ccTTCGCGCAGCTCTTTCGCGCCGGTCAGGTAGAAACCGCGCCATGTGGCGGACTCGGCCTGATGGCCCAGCTGTTCAAAGTTATTCGCTTGc                                                               >  2:3110777/1‑93 (MQ=255)

                           cTTCGCGCAGCTCTTTCGCGCCGGTCAGGTAGAAACCGCGCCATGTGGCGGACTCGGCCTGATGGCCCAGCTGTTCAAAGTTATTCGCTTGCa                                                              <  2:818408/93‑1 (MQ=255)

                           cTTCGCGCAGCTCTTTCGCGCCGGTCAGGTAGAAACCGCGCCATGTGGCGGACTCGGCCTGATGGCCCAGCTGTTCAAAGTTATTCGCTTGCa                                                              >  1:4922916/1‑93 (MQ=255)

                             tCGCGCAGCTCTTTCGCGCCGGTCAGGTAGAAACCGCGCCATGTGGCGGACTCGGCCTGATGGCCCAGCTGTTCAAAGTTATTCGCTTGCAGa                                                            <  2:4398678/93‑1 (MQ=255)

                             tCGCGCAGCTCTTTCGCGCCGGTCAGGTAGAAACCGCGCCATGTGGCGGACTCGGCCTGATGGCCCAGCTGTTCAAAGTTATTCGCTTGCAGa                                                            <  2:2767439/93‑1 (MQ=255)

                                 gcAGCTCTTTCGCGCCGGTCAGGTAGAAACCGCGCCATGTGGCGGACTCGGCCTGATGGCCCAGCTGTTCAAAGTTATTCGCTTGCAGAttct                                                        >  2:3430431/1‑93 (MQ=255)

                                 gcAGCTCTTTCGCGCCGGTCAGGTAGAAACCGCGCCATGTGGCGGACTCGGCCTGATGGCCCAGCTGTTCAAAGTTATTCGCTTGCAGAttct                                                        >  1:4085157/1‑93 (MQ=255)

                                    gCTCTTTCGCGCCGGTCAGGTAGAAACCGCGCCATGTGGCGGACTCGGCCTGATGGCCCAGCTGTTCAAAGTTATTCGCTTGCAGATTCTTcg                                                     <  2:207647/93‑1 (MQ=255)

                                     ctctTTCGCGCCGGTCAGGTAGAAACCGCGCCATGTGGCGGACTCGGCCTGATGGCCCAGCTGTTCAAAGTTATTCGCTTGCAGATTCTTcgc                                                    >  1:3024732/1‑93 (MQ=255)

                                      tctTTCGCGCCGGTCAGGTAGAAACCGCGCCATGTGGCGGACTCGGCCTGATGGCCCAGCTGTTCAAAGTTATTCGCTTGCAGATTCTTcgcg                                                   >  2:704184/1‑93 (MQ=255)

                                       ctTTCGCGCCGGTCAGGTAGAAACCGCGCCATGTGGCGGACTCGGCCTGATGGCCCAGCTGTTCAAAGTTATTCGCTTGCAGATTCTTCGGGa                                                  >  2:1739846/1‑93 (MQ=255)

                                        tttCGCGCCGGTCAGGTAGAAACCGCGCCATGTGGCGGACTCGGCCTGATGGCCCAGCTGTTCAAAGTTATTCGCTTGCAGATTCTTCGCGAc                                                 <  1:2892175/93‑1 (MQ=255)

                                         ttCGCGCCGGTCAGGTAGAAACCGCGCCATGTGGCGGACTCGGCCTGATGGCCCAGCTGTTCAAAGTTATTCGCTTGCAGATTCTTCGCGAcc                                                >  1:3357459/1‑93 (MQ=255)

                                          tCGCGCCGGTCAGGTAGAAACCGCGCCATGTGGCGGACTCGGCCTGATGGCCCAGCTGTTCAAAGTTATTCGCTTGCAGATTCTTCGCGACCt                                               <  1:2339726/93‑1 (MQ=255)

                                          tCGCGCCGGTCAGGTAGAAACCGCGCCATGTGGCGGACTCGGCCTGATGGCCCAGCTGTTCAAAGTTATTCGCTTGCAGATTCTTCGCGACCt                                               <  2:2582526/93‑1 (MQ=255)

                                          tCGCGCCGGTCAGGTAGAAACCGCGCCATGTGGCGGACTCGGCCTGATGGCCCAGCTGTTCAAAGTTATTCGCTTGCAGATTCTTCGCGACCt                                               <  2:2513870/93‑1 (MQ=255)

                                           cgcgCCGGTCAGGTAGAAACCGCGCCATGTGGCGGACTCGGCCTGATGGCCCAGCTGTTCAAAGTTATTCGCTTGCAGATTCTTCGCGACCTg                                              <  2:3247019/93‑1 (MQ=255)

                                            gcgcCGGTCAGGTCGAAACCGCGCCATGTGGCGGACTCGGCCTGATGGCCCAGCTGTTCAAAGTTATTCGCTTGCAGATTCTTCGCGACCTgg                                             <  2:3800498/93‑1 (MQ=255)

                                            gcgcCGGTCAGGTAGAAACCGCGCCATGTGGCGGACTCGGCCTGATGGCCCAGCTGTTCAAAGTTATTCGCTTGCAGATTCTTCGCGACCTgg                                             <  2:2199728/93‑1 (MQ=255)

                                              gcCGGTCAGGTAGAAACCGCGCCATGTGGCGGACTCGGCCTGATGGCCCAGCTGTTCAAAGTTATTCGCTTGCAGATTCTTCGCGACCTGGTc                                           >  2:1175572/1‑93 (MQ=255)

                                              gcCGGTCAGGTAGAAACCGCGCCATGTGGCGGACTCGGCCTGATGGCCCAGCTGTTCAAAGTTATTCGCTTGCAGATTCTTCGCGACCTGGTc                                           >  2:1884022/1‑93 (MQ=255)

                                               ccGGTCAGGTAGAAACCGCGCCATGTGGCGGACTCGGCCTGATGGCCCAGCTGTTCAAAGTTATTCGCTTGCAGATTCTTCGCGACCTGGTCa                                          <  1:5051287/93‑1 (MQ=255)

                                               ccGGTCAGGTAGAAACCGCGCCATGTGGCGGACTCGGCCTGATGGCACAGCTGTTCAAAGCTATTCGCTTGCAGATTCTTCGCGACCTGGTcc                                          >  2:5493487/1‑92 (MQ=255)

                                                 ggTCAGGTAGAAACCGCGCCATGTGGCGGACTCGGCCTGATGGCCCAGCTGTTCAAAGTTATTCGCTTGCAGATTCTTCGCGACCTGGTCAcc                                        <  2:162142/93‑1 (MQ=255)

                                                 ggTCAGGTAGAAACCGCGCCATGTGGCGGACTCGGCCTGATGGCCCAGCTGTTCAAAGTTATTCGCTTGCAGATTCTTCGCGACCTGGTCAcc                                        >  1:6029433/1‑93 (MQ=255)

                                                  gTCAGGTAGAAACCGCGCCATGTGGCGGACTCGTCCTGATGGCCCAGCTGTTCAAAGTTATTCGCTTGCAGATTCTTCGCGACCTGGTCAccc                                       <  2:6651018/93‑1 (MQ=255)

                                                  gTCAGGTAGAAACCGCGCCATGTGGCGGACTCGGCCTGATGGCCCAGCTGTTCAAAGTTATTCGCTTGCAGATTCTTCGCGACCTGGTCAccc                                       >  1:1354331/1‑93 (MQ=255)

                                                    cAGGTAGAAACCGCGCCATGTGGCGGACTCGGCCTGATGGCCCAGCTGTTCAAAGTTATTCGCTTGCAGATTCTTCGCGACCTGGTCACCCgg                                     >  2:4638276/1‑93 (MQ=255)

                                                     aGGTAGAAACCGCGCCATGTGGCGGACTCGGCCTGATGGCCCAGCTGTTCAAAGTTATTCGCTTGCAGATTCTTCGCGACCTGGTCACCCggg                                    >  2:4712505/1‑93 (MQ=255)

                                                      ggTAGAAACCGCGCCATGTGGCGGACTCGGCCTGATGGCCCAGCTGTTCAAAGTTATTCGCTTGCAGATTCTTCGCGACCTGGTCACCCGGGt                                   <  1:6175334/93‑1 (MQ=255)

                                                      ggTAGAAACCGCGCCATGTGGCGGACTCGGCCTGATGGCCCAGCTGTTCAAAGTTATTCGCTTGCAGATTCTTCGCGACCTGGTCACCCGGGt                                   <  2:565350/93‑1 (MQ=255)

                                                      ggTAGAAACCGCGCCATGTGGCGGACTCGGCCTGATGGCCCAGCTGTTCAAAGTTATTCGCTTGCAGATTCTTCGCGACCTGGTCACCCGGGt                                   <  2:4321969/93‑1 (MQ=255)

                                                       gTAGAAACCGCGCCATGTGGCGGACTCGGCCTGATGGCCCAGCTGTTCAAAGTTATTCGCTTGCAGATTCTTCGCGACCTGGTCACCCGGGtt                                  >  1:6429606/1‑93 (MQ=255)

                                                        tAGAAACCGCGCCATGTGGCGGACTCGGCCTGATGGCCCAGCTGTTCAAAGTTATTCGCTTGCAGATTCTTCGCGACCTGGTCACCCGGGTTg                                 <  2:6027632/93‑1 (MQ=255)

                                                        tAGAAACCGCGCCATGTGGCGGACTCGGCCTGATGGCCCAGCTGTTCAAAGTTATTCGCTTGCAGATTCTTCGCGACCTGGTCACCCGGGTTg                                 >  1:2094098/1‑93 (MQ=255)

                                                         aGAAACCGCGCCATGTGGCGGACTCGGCCTGATGGCCCAGCTGTTCAAAGTTATTCGCTTGCAGATTCTTCGCGACCTGGTCACCCGGGTTgg                                <  1:3480593/93‑1 (MQ=255)

                                                          gAAACCGCGCGATGTGGGGGACTCGGCGTGATGGCCCAGCTGTTCAAAGTTATTCGCTTGCAGATTCTTCGCGACCTGGTCACCCGGGTTggc                               <  1:4365929/93‑1 (MQ=255)

                                                          gAAACCGCGCCATGTGGCGGACTCGGCCTGATGGCCCAGCTGTTCAAAGTTATTCGCTTGCAGATTCTTCGCGACCTGGTCACCCGGGTTggg                               >  1:997887/1‑92 (MQ=255)

                                                          gAAACCGCGCCATGTGGCGGACTCGGCCTGATGGCCCAGCTGTTCAAAGTTATTCGCTTGCAGATTCTTCGCGACCTGGTCACCCGGGTTggc                               <  2:5111460/93‑1 (MQ=255)

                                                          gAAACCGCGCCATGTGGCGGACTCGGCCTGATGGCCCAGCTGTTCAAAGTTATTCGCTTGCAGATTCTTCGCGACCTGGTCACCCGGGTTggc                               <  1:902345/93‑1 (MQ=255)

                                                            aaCCGCGCCATGTGGCGGACTCGGCCTGATGGCCCAGCTGTTCAAAGTTATTCGCTTGCAGATTCTTCGCGACCTGGTCACCCGGGTTggcgg                             >  2:5011701/1‑93 (MQ=255)

                                                             aCCGCGCCATGTGGCGGACTCGGCCTGATGGCCCAGCTGTTCAAAGTTATTCGCTTGCAGATTCTTCGCGACCTGGTCACCCGGGTTggcggc                            <  1:563653/93‑1 (MQ=255)

                                                              ccGCGCCATGTGGCGGACTCGGCCTGATGGCCCAGCTGTTCAAAGTTATTCGCTTGCAGATTCTTCGCGACCTGGTCACCCGGGTTGGCGGCa                           >  2:86454/1‑93 (MQ=255)

                                                              ccGCGCCATGTGGCGGACTCGGCCTGATGGCCCAGCTGTTCAAAGTTATTCGCTTGCAGATTCTTCGCGACCTGGTCACCCGGGTTGGCGGCa                           >  1:3920578/1‑93 (MQ=255)

                                                                     aTGTGGCGGACTCGGCCTGATGGCCCAGCTGTTCAAAGTTATTCGCTTGCAGATTCTTCGCGACCTGGTCACCCGGGTTGGCGGCAATCACCt                    >  2:366638/1‑93 (MQ=255)

                                                                      tgtgGCGGACTCGGCCTGATGGCCCAGCTGTTCAAAGTTATTCGCTTGCAGATTCTTCGCGACCTGGTCACCCGGGTTGGCGGCAATCACCTg                   <  1:452519/93‑1 (MQ=255)

                                                                       gtgGCGGACTCGGCCTGATGGCCCAGCTGTTCAAAGTTATTCGCTTGCAGATTCTTCGCGACCTGGTCACCCGGGTTGGCGGCAATCACCTGt                  <  1:2410936/93‑1 (MQ=255)

                                                                       gtgGCGGACTCGGCCTGATGGCCCAGCTGTTCAAAGTTATTCGCTTGCAGATTCTTCGCGACCTGGTCACCCGGGTTGGCGGCAATCACCTGt                  <  2:4813133/93‑1 (MQ=255)

                                                                       gtgGCGGACTCGGCCTGATGGCCCAGCTGTTCAAAGTTATTCGCTTGCAGATTCTTCGCGACCTGGTCACCCGGGTTGGCGGCAATCACCTGt                  <  1:4333599/93‑1 (MQ=255)

                                                                         ggCGGACTCGGCCTGATGGCCCAGCTGTTCAAAGTTATTCGCTTGCAGATTCTTCGCGACCTGGTCACCCGGGTTGGCGGCAATCACCTGttt                >  2:3913895/1‑93 (MQ=255)

                                                                          gCGGACTCGTCCTGATGGCCCAGCTGTTCAAAGTTATTCGCTTGCAGATTCTTCGCGACCTGGTCACCCGGGTTGGCGGCAATCACCTGTTTc               <  1:5095018/93‑1 (MQ=255)

                                                                            ggACTCGGCCTGATGGCCCAGCTGTTCAAAGTTATTCGCTTGCAGATTCTTCGCGACCTGGTCACCCGGGTTGGCGGCAATCACCTGTTTcag             >  2:5309599/1‑93 (MQ=255)

                                                                            ggACTCGGCCTGATGGCCCAGCTGTTCAAAGTTATTCGCTTGCAGATTCTTCGCGACCTGGTCACCCGGGTTGGCGGCAATCACCTGTTTcag             <  1:5455383/93‑1 (MQ=255)

                                                                             gACTCGGCCTGATGGCCCAGCTGTTCAAAGTTATTCGCTTGCAGATTCTTCGCGACCTGGTCACCCGGGTTGGCGGCAATCACCTGTTTcagc            >  2:1130934/1‑93 (MQ=255)

                                                                             gACTCGGCCTGATGGCCCAGCTGTTCAAAGTTATTCGCTTGCAGATTCTTCGCGACCTGGTCACCCGGGTTGGCGGCAATCACCTGTTTcagc            <  2:5322731/93‑1 (MQ=255)

                                                                             gACTCGGCCTGATGGCCCAGCTGTTCAAAGTTATTCGCTTGCAGATTCTTCGCGACCCGGTCACCCGCGTTGACGGCAATCACCTGGTTcacc            >  2:6438189/1‑91 (MQ=255)

                                                                              aCTCGGCCTGATGGCCCAGCTGTTCAAAGTTATTCGCTTGCAGATTCTTCGCGACCTGGTCACCCGGGTTGGCGGCAATCACCTGTTTcagca           <  1:3173384/93‑1 (MQ=255)

                                                                               cTCGGCCTGATGGCCCAGCTGTTCAAAGTTATTCGCTTGCAGATTCTTCGCGACCTGGTCACCCGGGTTGGCGGCAATCACCTGTTTcagcag          >  2:336506/1‑93 (MQ=255)

                                                                                tCGGCCTGATGGCCCAGCTGTTCAAAGTTATTCGCTTGCAGATTCTTCGCGACCTGGTCACCCGGGTTGGCGGCAATCACCTGTTTCAGCAGt         <  1:4040673/93‑1 (MQ=255)

                                                                                 cGGCCTGATGGCCCAGCTGTTCAAAGTTATTCGCTTGCAGATTCTTCGCGACCTGGTCACCCGGGTTGGCGGCAATCACCTGTTTCAGCAGtt        <  2:5710637/93‑1 (MQ=255)

                                                                                  ggCCTGATGGCCCAGCTGTTCAAAGTTATTCGCTTGCAGATTCTTCGCGACCTGGTCACCCGGGTTGGCGGCAATCACCTGTTTCAGCAGTTc       >  1:3098554/1‑93 (MQ=255)

                                                                                  ggCCTGATGGCCCAGCTGTTCAAAGTTATTCGCTTGCAGATTCTTCGCGACCTGGTCACCCGGGTTGGCGGCAATCACCTGTTTCAGCAGTTc       <  1:3563691/93‑1 (MQ=255)

                                                                                   gCCTGATGGCCCAGCTGTTCAAAGTTATTCGCTTGCAGATTCTTCGCGACCTGGTCACCCGGGTTGGCGGCAATCACCTGTTTCAGCAGTTCt      <  1:1435976/93‑1 (MQ=255)

                                                                                   gCCTGATGGCCCAGCTGTTCAAAGTTATTCGCTTGCAGATTCTTCGCGACCTGGTCACCCGGGTTGGCGGCAATCACCTGTTTCAGCAGTTCt      >  2:1842640/1‑93 (MQ=255)

                                                                                    ccTGATGGCCCAGCTGTTCAAAGTTATTCGCTTGCAGATTCTTCGCGACCTGGTCACCCGGGTTGGCGGCAATCACCTGTTTCAGCAGTTCTg     >  2:1963743/1‑93 (MQ=255)

                                                                                     cTGATGGCCCAGCTGTTCAAAGTTATTCGCTTGCAGATTCTTCGCGACCTGGTCACCCGGGTTGGCGCCAATCACCTGTTTCAGCAGTTCTGc    >  2:2439633/1‑93 (MQ=255)

                                                                                      tGATGGCCCAGCTGTTCAAAGTTATTCGCTTGCAGATTCTTCGCGACCTGGTCACCCGGGTTGGCGGCAATCACCTGTTTCAGCAGTTCTGcc   >  2:2970901/1‑93 (MQ=255)

                                                                                      tGATGGCCCAGCTGTTCAAAGTTATTCGCTTGCAGATTCTTCGCGACCTGGTCACCCGGGTTGGCGGCAATCACCTGTTTCAGCAGTTCTGcc   >  1:189638/1‑93 (MQ=255)

                                                                                       gATGGCCCAGCTGTTCAAAGTTATTCGCTTGCAGATTCTTCGCGACCTGGTCACCCGGGTTGGCGGCAATCACCTGTTTCAGCAGTTCTGCCg  >  2:5290651/1‑93 (MQ=255)

                                                                                       gATGGCCCAGCTGTTCAAAGTTATTCGCTTGCAGATTCTTCGCGACCTGGTCACCCGGGTTGGCGGCAATCACCTGTTTCAGCAGTTCTGCCg  <  2:596907/93‑1 (MQ=255)

                                                                                       gATGGCCCAGCTGTTCAAAGTTATTCGCTTGCAGATTCTTCGCGACCTGGTCACCCGGGTTGGCGGCAATCACCTGCTTCAGCAGTTCTGCCg  >  1:4531721/1‑93 (MQ=255)

TGGTGCCGTGGCTGAACTTATGCACCCCTTCGCGCAGCTCTTTCGCGCCGGTCAGGTAGAAACCGCGCCATGTGGCGGACTCGGCCTGATAGCCCAGCTGTTCAAAGTTATTCGCTTGCAGATTCTTCGCGACCTGGTCACCCGGGTTGGCGGCAATCACCTGTTTCAGCAGTTCTGCCG  >  NC_000913/4302562‑4302741

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 19 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`