BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,372,457	A→G	V260A (GTG→GCG)	yjeK ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,372,457	0	A	G	99.7%	964.7 / ‑4.8	297	V260A (GTG→GCG)	yjeK	hypothetical protein
Reads supporting (aligned to +/- strand): ref base A (0/1); new base G (135/160); total (136/161)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.10e-01

Only 100 of 302 total aligned reads displayed.

ACTTTATCGAGCACATGCAGGTAATACGGCATTACGCCGGCATCGAACAACGCATTACTCAGGTTTGCCAGCGTTTGTGCGTTATCGTTCACATCACGTAACAGAACGCTCTGGTTCAGCAAAGTAACGCCTACCCGGCGCAACTTAGCCATCGCCTGACGGAATGTTTCATCTACCTCA  >  NC_000913/4372367‑4372546

aCTTTATCGAGCACATGCAGGTAATACGGCATTACGCCGGCATCGAACAACGCATTACTCAGGTTTGCCAGCGTTTGTGCGTTATCGTTcgca                                                                                         >  2:1503805/1‑93 (MQ=255)

aCTTTATCGAGCACATGCAGGTAATACGGCATTACGCCGGCATCGAACAACGCATTACTCAGGTTTGCCAGCGTTTGTGCGTTATCGTTcgca                                                                                         <  2:2067225/93‑1 (MQ=255)

    tATCGAGCACATGCAGGTAATACGGCATTACGCCGGCATCGAACAACGCATTACTCAGGTTTGCCAGCGTTTGTGCGTTATCGTTCGCATCAc                                                                                     <  2:170571/93‑1 (MQ=255)

    tATCGAGCACATGCAGGTAATACGGCATTACGCCGGCATCGAACAACGCATTACTCAGGTTTGCCAGCGTTTGTGCGTTATCGTTCGCATCAc                                                                                     <  1:588346/93‑1 (MQ=255)

     aTCGAGCACATGCAGGTAATACGGCATTACGCCGGCATCGAACAACGCATTACTCAGGTTTGCCAGCGTTTGTGCGTTATCGTTCGCATCACg                                                                                    >  1:3105892/1‑93 (MQ=255)

         aGCACATGCAGGTAATACGGCATTACGCCGGCATCGAACAACGCATTACTCAGGTTTGCCAGCGTTTGTGCGTTATCGTTCGCATCACGTAAc                                                                                <  2:2496217/93‑1 (MQ=255)

          gCACATGCAGGTAATACGGCATTACGCCGGCATCGAACAACGCATTACTCAGGTTTGCCAGCGTTTGTGCGTTATCGTTCGCATCACGTAACa                                                                               >  2:6628117/1‑93 (MQ=255)

          gCACATGCAGGTAATACGGCATTACGCCGGCATCGAACAACGCATTACTCAGGTTTGCCAGCGTTTGTGCGTTATCGTTCGCATCACGTAACa                                                                               <  1:3038335/93‑1 (MQ=255)

            acaTGCAGGTAATACGGCATTACGCCGGCATCGAACAACGCATTACTCAGGTTTGCCAGCGTTTGTGCGTTATCGTTCGCATCACGTAACAGa                                                                             >  1:4487099/1‑93 (MQ=255)

            acaTGCAGGTAATACGGCATTACGCCGGCATCGAACAACGCATTACTCAGGTTTGCCAGCGTTTGTGCGTTATCGTTCGCATCACGTAACAGa                                                                             >  2:3923180/1‑93 (MQ=255)

             caTGCAGGTAATACGGCATTACGCCGGCATCGAACAACGCATTACTCAGGTTTGCCAGCGTTTGTGCGTTATCGTTCGCATCACGTAACAGaa                                                                            >  1:1952578/1‑93 (MQ=255)

              aTGCAGGTAATACGGCATTACGCCGGCATCGAACAACGCATTACTCAGGTTTGCCAGCGTTTGTGCGTTATCGTTCGCATCACGTAACAGAAc                                                                           <  2:6359228/93‑1 (MQ=255)

                gCAGGTAATACGGCATTACGCCGGCATCGAACAACGCATTACTCAGGTTTGCCAGCGTTTGTGCGTTATCGTTCGCATCACGTAACAGAACGc                                                                         <  1:5151326/93‑1 (MQ=255)

                gCAGGTAATACGGCATTACGCCGGCATCGAACAACGCATTACTCAGGTTTGCCAGCGTTTGTGCGTTATCGTTCGCATCACGTAACAGAACGc                                                                         <  2:4696005/93‑1 (MQ=255)

                  aGGTAATACGGCATTACGCCGGCATCGAACAACGCATTACTCAGGTTTGCCAGCGTTTGTGCGTTATCGTTCGCATCACGTAACAGAACGctc                                                                       <  2:1253311/93‑1 (MQ=255)

                  aGGTAATACGGCATTACGCCGGCATCGAACAACGCATTACTCAGGTTTGCCAGCGTTTGTGCGTTATCGTTCGCATCACGTAACAGAACGctc                                                                       <  2:4449902/93‑1 (MQ=255)

                   ggTAATACGGCATTACGCCGGCATCGAACAACGCATTACTCAGGTTTGCCAGCGTTTGTGCGTTATCGTTCGCATCACGTAGCAGAACGctct                                                                      <  2:5839579/93‑1 (MQ=255)

                   ggTAATACGGCATTACGCCGGCATCGAACAACGCATTACTCAGGTTTGCCAGCGTTTGTGCGTTATCGTTCGCATCACGTAACAGAACGctct                                                                      >  1:3904681/1‑93 (MQ=255)

                   ggTAATACGGCATTACGCCGGCATCGAACAACGCATTACTCAGGTTTGCCAGCGTTTGTGCGTTATCGTTCGCATCACGTAACAGAACGctct                                                                      >  1:1459127/1‑93 (MQ=255)

                    gTAATACGGCATTACGCCGGCATCGAACAACGCATTACTCAGGTTTGCCAGCGTTTGTGCGTTATCGTTCGCATCACGTAACAGAACGCTCTg                                                                     >  2:4471381/1‑93 (MQ=255)

                    gTAATACGGCATTACGCCGGCATCGAACAACGCATTACTCAGGTTTGCCAGCGTTTGTGCGTTATCGTTCGCATCACGTAACAGAACGCTCTg                                                                     <  2:5353794/93‑1 (MQ=255)

                     tAATACGGCATTACGCCGGCATCGAACAACGCATTACTCAGGTTTGCCAGCGTTTGTGCGTTAGCGTTCGCATCACGTAACAGAACGCTCTgg                                                                    <  1:2671443/93‑1 (MQ=255)

                      actACGGCATTCCGCCGGTATCGAACAACGCATTACTCAGGTTTGCCAGCGTTTGTACGTTATCGTTCGCATCACGTAACATAACGCTCTGGt                                                                   <  1:4825754/91‑1 (MQ=255)

                      aaTACGGCATTACGCCGGCATCGAACAACGCATTACTCAGGTTTGCCAGCGTTTGTGCGTTATCGTTCGCATCACGTAACAGAACGCTCTGGt                                                                   >  1:3235627/1‑93 (MQ=255)

                       aTACGGCATTACGCCGGCATCGAACAACGCATTACTCAGGTTTGCCAGCGTTTGTGCGTTATCGTTCGCATCACGTAACAGAACGCTCTGGtt                                                                  <  1:2618039/93‑1 (MQ=255)

                       aTACGGCATTACGCCGGCATCGAACAACGCATTACTCAGGTTTGCCAGCGTTTGTGCGTTATCGTTCGCATCACGTAACAGAACGCTCTGGtt                                                                  >  1:190575/1‑93 (MQ=255)

                         aCGGCATTACGCCGGCATCGAACAACGCATTACTCAGGTTTGCCAGCGTTTGTGCGTTATCGTTCGCATCACGTAACAGAACGCTCTGGTTCa                                                                <  2:3602995/93‑1 (MQ=255)

                         aCGGCATTACGCCGGCATCGAACAACGCATTACTCAGGTTTGCCAGCGTTTGTGCGTTATCGTTCGCATCACGTAACAGAACGCTCTGGTTCa                                                                <  1:5707783/93‑1 (MQ=255)

                          cGGCATTACGCCGGCATCGAACAACGCATTACTCAGGTTTGCCAGCGTTTGTGCGTTATCGTTCGCATCACGTAACAGAACGCTCTGGTTCAg                                                               >  1:4779475/1‑93 (MQ=255)

                          cGGCACTACGCCGGCATCGAACAGCGCATTACTCAGGTTTGCCAGCGTTTGTGCGTTATCGTTCGCATCACGTAACAGAACGCTCTGGTTCAg                                                               <  2:3447588/93‑1 (MQ=255)

                              aTTACGCCGGCATCGAACAACGCATTACTCAGGTTTGCCAGCGTTTGTGCGTTATCGTTCGCATCACGTAACAGAACGCTCTGGTTCAGCaaa                                                           >  1:5980071/1‑93 (MQ=255)

                              aTTACGCCGGCATCGAACAACGCATTACTCAGGTTTGCCAGCGTTTGTGCGTTATCGTTCGCATCACGTAACAGAACGCTCTGGTTCAGCaaa                                                           <  1:6624085/93‑1 (MQ=255)

                                tACGCCGGCATCGAACAACGCATTACTCAGGTTTGCCAGCGTTTGTGCGTTATCGTTCGCATCACGTAACAGAACGCTCTGGTTCAGCAAAGt                                                         >  1:5558472/1‑93 (MQ=255)

                                       gCATCGAACAACGCATTACTCAGGTTTGCCAGCGTTTGTGCGTTATCGTTCGCATCACGTAACAGAACGCTCTGGTTCAGCAAAGTAACGCCt                                                  >  1:685004/1‑93 (MQ=255)

                                        cATCGAACAACGCATTACTCAGGTTTGCCAGCGTTTGTGCGTTATCGTTCGCATCACGTAACAGAACGCTCTGGTTCAGCAAAGTAACGCCTa                                                 >  1:3501282/1‑93 (MQ=255)

                                         aTCGAACAACGCATTACTCAGGTTTGCCAGCGTTTGTGCGTTATCGTTCGCATCACGTAACAGAACGCTCTGGTTCAGCAAAGTAACGCCTAc                                                >  1:3678401/1‑93 (MQ=255)

                                         aTCGAACAACGCATTACTCAGGTTTGCCAGCGTTTGTGCGTTATCGTTCGCATCACGTAACAGAACGCTCTGGTTCAGCAAAGTAACGCCTAc                                                >  2:5203701/1‑93 (MQ=255)

                                         aTCGAACAACGCATTACTCAGGTTTGCCAGCGTTTGTGCGTTATCGTTCGCATCACGTAACAGAACGCTCTGGTTCAGCAAAGTAACGCCTAc                                                >  2:860101/1‑93 (MQ=255)

                                          tCGAACAACGCATTACTCAGGTTTGCCAGCGTTTGTGCGTTATCGTTCGCATCACGTAACAGAACGCTCTGGTTCAGCAAAGTAACGCCTAcc                                               >  1:504988/1‑93 (MQ=255)

                                          tCGAACAACGCATTACTCAGGTTTGCCAGCGTTTGTGCGTTATCGTTCGCATCACGTAACAGAACGCTCTGGTTCAGCAAAGTAACGCCTAcc                                               >  2:5676097/1‑93 (MQ=255)

                                           cGAACAACGCATTACTCAGGTTTGCCAGCGTTTGTGCGTTATCGTTCGCATCACGTAACAGAACGCTCTGGTTCAGCAAAGTAACGCCTAccc                                              >  1:5318144/1‑93 (MQ=255)

                                                aacGCATTACTCAGGTTTGCCAGCGTTTGTGCGTTATCGTTCGCATCACGTAACAGAACGCTCTGGTTCAGCAAAGTAACGCCTACCCGgcgc                                         <  1:5856884/93‑1 (MQ=255)

                                                 acGCATTACTCAGGTTTGCCAGCGTTTGTGCGTTATCGTTCGCATCACGTAACAGAACGCTCTGGTTCAGCAAAGTAACGCCTACCCGGCGCa                                        >  2:2173643/1‑93 (MQ=255)

                                                    cATTACTCAGGTTTGCCAGCGTTTGTGCGTTATCGTTCGCATCACGTAACAGAACGCTCTGGTTCAGCAAAGTAACGCCTACCCGGCGCAACt                                     >  2:2920869/1‑93 (MQ=255)

                                                    cATTACTCAGGTTTGCCAGCGTTTGTGCGTTATCGTTCGCATCACGTAACAGAACGCTCTGGTTCAGCAAAGTAACGCCTACCCGGCGCAACt                                     >  2:5724926/1‑93 (MQ=255)

                                                    cATTACTCAGGTTTGCCAGCGTTTGTGCGTTATCGTTCGCATCACGTAACAGAACGCTCTGGTTCAGCAAAGTAACGCCTACCCGGCGCAACt                                     <  1:1569602/93‑1 (MQ=255)

                                                      ttACTCAGGTTTGCCAGCGTTTGTGCGTTATCGTTCGCATCACGTAACAGAACGCTCTGGTTCAGCAAAGTAACGCCTACCCGGCGCAACTTa                                   >  2:1053781/1‑93 (MQ=255)

                                                      ttACTCAGGTTTGCCAGCGTTTGTGCGTTATCGTTCGCATCACGTAACAGAACGCTCTGGTTCAGCAAAGTAACGCCTACCCGGCGCAACTTa                                   <  1:1282224/93‑1 (MQ=255)

                                                       tACTCAGGTTTGCCAGCGTTTGTGCGTTATCGTTCGCATCACGTAACAGAACGCTCTGGTTCAGCAAAGTAACGCCTACCCGGCGCAACTTAg                                  >  1:5100419/1‑93 (MQ=255)

                                                        aCTCAGGTTTGCCAGCGTTTGTGCGTTATCGTTCGCATCACGTAACAGAACGCTCTGGTTCAGCAAAGTAACGCCTACCCGGCGCAACTTAGc                                 >  2:4375023/1‑93 (MQ=255)

                                                        aCTCAGGTTTGCCAGCGTTTGTGCGTTATCGTTCGCATCACGTAACAGAACGCTCTGGTTCAGCAAAGTAACGCCTACCCGGCGCAACTTAGc                                 <  1:1306164/93‑1 (MQ=255)

                                                        aCTCAGGTTTGCCAGCGTTTGTGCGTTATCGTTCGCATCACGTAACAGAACGCTCTGGTTCAGCAAAGTAACGCCTACCCGGCGCAACTTAGc                                 <  2:4763885/93‑1 (MQ=255)

                                                        aCTCAGGTTTGCCAGCGTTTGTGCGTTATCGTTCGCATCACGTAACAGAACGCTCTGGTTCAGCAAAGTAACGCCTACCCGGCGCAACTTAGc                                 >  2:2544140/1‑93 (MQ=255)

                                                        aCTCAGGTTTGCCAGCGTTTGTGCGTTACCGTTCGCATCACGTAACAGAACGCTCTGGTTCAGCAAAGTAACGCCTACCCGGCGCAACTTAGc                                 >  1:2212019/1‑93 (MQ=255)

                                                         cTCAGGTTTGCCAGCGTTTGTGCGTTATCGTTCGCATCACGTAACAGAACGCTCTGGTTCAGCAAAGTAACGCCTACCCGGCGCAACTTAGcc                                >  1:4696312/1‑93 (MQ=255)

                                                           cAGGTTTGCCAGCGTTTGTGCGTTATCGTTCGCATCACGTAACAGAACGCTCTGGTTCAGCAAAGTAACGCCTACCCGGCGCAACTTAGCCAt                              <  1:2447704/93‑1 (MQ=255)

                                                              gTTTGCCAGCGTTTGTGCGTTATCGTTCGCATCACGTAACAGAACGCTCTGGTTCAGCAAAGTAACGCCTACCCGGCGCAACTTAGCCATCGc                           <  2:3044158/93‑1 (MQ=255)

                                                               tttGCCAGCGTTTGTGCGTTATCGTTCGCATCACGTAACAGAACGCTCTGGTTCAGCAAAGTAACGCCTACCCGGCGCCACTTGGGCCTCGcc                          >  1:1785907/1‑93 (MQ=255)

                                                               tttGCCAGCGTTTGTGCGTTATCGTTCGCATCACGTAACAGAACGCTCTGGTTCAGCAAAGTAACGCCTACCCGGCGCAACTTAGCCATCGcc                          >  2:6208341/1‑93 (MQ=255)

                                                                ttGCCAGCGTTTGTGCGTTATCGTTCGCATCACGTAACAGAACGCTCTGGTTCAGCAAAGTAACGCCTACCCGGCGCAACTTAGCCATCGCCt                         >  1:3334752/1‑93 (MQ=255)

                                                                ttGCCAGCGTTTGTGCGTTATCGTTCGCATCACGTAACAGAACGCTCTGGTTCAGCAAAGTAACGCCTACCCGGCGCAACTTAGCCATCGCCt                         <  1:2954278/93‑1 (MQ=255)

                                                                 tGCCAGCGTTTGTGCGTTATCGTTCGCATCACGTAACAGAACGCTCTGGTTCAGCAAAGTAACGCCTACCCGGCGCAACTTAGCCATCGCCTg                        >  2:4175969/1‑93 (MQ=255)

                                                                 tGCCAGCGTTTGTGCGTTATCGTTCGCATCACGTAACAGAACGCTCTGGTTCAGCAAAGTAACGCCTACCCGGCGCAACTTAGCCATCGCCTg                        <  2:3255132/93‑1 (MQ=255)

                                                                  gCCAGCGTTTGTGCGTTATCGTTCGCATCACGTAACAGAACGCTCTGGTTCAGCAAAGTAACGCCTACCCGGCGCAACTTAGCCATCGCCTGa                       >  1:938544/1‑93 (MQ=255)

                                                                   ccAGCGTTTGTGCGTTATCGTTCGCATCACGTAACAGAACGCTCTGGTTCAGCAAAGTAACGCCTACCCGGCGCAACTTAGCCATCGCCTGAc                      <  2:177566/93‑1 (MQ=255)

                                                                   ccAGCGTTTGTGCGTTATCGTTCGCATCACGTAACAGAACGCTCTGGTTCAGCAAAGTAACGCCTACCCGGCGCAACTTAGCCATCGCCTGAc                      >  2:3146718/1‑93 (MQ=255)

                                                                   ccAGCGTTTGTGCGTTATCGTTCGCATCACGTAACAGAACGCTCTGGTTCAGCAAAGTAACGCCTACCCGGCGCAACTTAGCCATCGCCTGAc                      >  2:6681916/1‑93 (MQ=255)

                                                                    cAGCGTTTGTGCGTTATCGTTCGCATCACGTAACAGAACGCTCTGGTTCAGCAAAGTAACGCCTACCCGGCGCAACTTAGCCATCGCCTGACg                     <  2:724575/93‑1 (MQ=255)

                                                                    cAGCGTTTGTGCGTTATCGTTCGCATCACGTAACAGAACGCTCTGGCTCAGCAAAGGAACGCCTACCCGGCGCAACTTAGCCATCGCCTGACg                     >  1:437686/1‑93 (MQ=255)

                                                                     aGCGTTTGTGCGTTATCGTTCGCATCACGTAACAGAACGCTCTGGTTCAGCAAAGTAACGCCTACCCGGCGCAACTTAGCCATCGCCTGACgg                    <  2:5547171/93‑1 (MQ=255)

                                                                     aGCGTTTGTGCGTTATCGTTCGCATCACGTAACAGAACGCTCTGGTTCAGCAAAGTAACGCCTACCCGGCGCAACTTAGCCATCGCCTGACgg                    <  1:1657931/93‑1 (MQ=255)

                                                                      gCGTTTGTGCGTTATCGTTCGCATCACGTAACAGAACGCTCTGGTTCAGCAAAGTAACGCCTACCCGGCGCAACTTAGCCATCGCCTGACGGa                   <  2:547899/93‑1 (MQ=255)

                                                                      gCGTTTGTGCGTTATCGTTCGCATCACGTAACAGAACGCTCTGGTTCAGCAAAGTAACGCCTACCCGGCGCAACTTAGCCATCGCCTGACGGa                   >  2:1617607/1‑93 (MQ=255)

                                                                       cGTTTGTGCGTTATCGTTCGCATCACGTAACAGAACGCTCTGGTTCAGCAAAGTAACGCCTACCCGGCGCAACTTAGCCATCGCCTGACGGaa                  <  1:497237/93‑1 (MQ=255)

                                                                       cGTTTGTGCGTTATCGTTCGCATCACGTAACAGAACGCTCTGGTTCAGCAAAGTAACGCCTACCCGGCGCAACTTAGCCATCGCCTGACGGaa                  <  2:4983552/93‑1 (MQ=255)

                                                                         tttGTGCGTTATCGTTCGCATCACGTAACAGAACGCTCTGGTTCAGCAAAGTAACGCCTACCCGGCGCAACTTAGCCATCGCCTGACGGAATg                <  1:6456421/93‑1 (MQ=255)

                                                                          ttGTGCGTTATCGTTCGCATCACGTAACAGAACGCTCTGGTTCAGCAAAGTAACGCCTACCCGGCGCAACTTAGCCATCGCCTGACGGAATGt               <  1:4570323/93‑1 (MQ=255)

                                                                          ttGTGCGTTATCGTTCGCATCACGTAACAGAACGCTCTGGTTCAGCAAAGTAACGCCTACCCGGCGCAACTTAGCCATCGCCTGACGGAATGt               <  2:3727046/93‑1 (MQ=255)

                                                                           tgtgCGTTATCGTTCGCATCACGTAACAGAACGCTCTGGTTCAGCAAAGTAACGCCTACCCGGCGCAACTTAGCCATCGCCTGACGGAATGtt              <  2:3399141/93‑1 (MQ=255)

                                                                           tgtgCGTTATCGTTCGCATCACGTAACAGAACGCTCTGGTTCAGCAAAGTAACGCCTACCCGGCGCAACTTAGCCATCGCCTGACGGAATGtt              <  2:4945424/93‑1 (MQ=255)

                                                                           tgtgCGTTATCGTTCGCATCACGTAACAGAACGCTCTGGTTCAGCAAAGTAACGCCTACCCGGCGCAACTTAGCCATCGCCTGACGGAATGtt              <  1:5191070/93‑1 (MQ=255)

                                                                            gtgCGTTATCGTTCGCATCACGTAACAGAACGCTCTGGTTCAGCAAAGTAACGCCTACCCGGCGCAACTTAGCCATCGCCTGACGGAATGttt             <  1:284829/93‑1 (MQ=255)

                                                                             tgCGTTATCGTTCGCATCACGTAACAGAACGCTCTGGTTCAGCAAAGTAACGCCTACCCGGCGCAACTTAGCCATCGCCTGACGGAATGTTTc            <  1:4619493/93‑1 (MQ=255)

                                                                             tgCGTTATCGTTCGCATCACGTAACAGAACGCTCTGGTTCAGCAAAGTAACGCCTACCCGGCGCAACTTAGCCATCGCCTGACGGAATGTTTc            >  2:647742/1‑93 (MQ=255)

                                                                              gCGTTATCGTTCGCATCACGTAACAGAACGCTCTGGTTCAGCAAAGTAACGCCTACCCGGCGCAACTTAGCCATCGCCTGACGGAATGTTTCa           <  2:5951056/93‑1 (MQ=255)

                                                                              gCGTTATCGTTCGCATCACGTAACAGAACGCTCTGGTTCAGCAAAGTAACGCCTACCCGGCGCAACTTAGCCATCGCCTGACGGAATGTTTCa           <  2:2782195/93‑1 (MQ=255)

                                                                                gTTATCGTTCGCATCACGTAACAGAACGCTCTGGTTCAGCAAAGTAACGCCTACCCGGCGCAACTTAGCCATCGCCTGACGGAATGTTTCATc         <  1:6297533/93‑1 (MQ=255)

                                                                                gTTATCGTTCGCATCACGTAACAGAACGCTCTGGTTCAGCAAAGTAACGCCTACCCGGCGCAACTTAGCCATCGCCTGACGGAATGTTTCATc         <  2:6654332/93‑1 (MQ=255)

                                                                                gTTATCGTTCGCATCACGTAACAGAACGCTCTGGTTCAGCAAAGTAACGCCTACCCGGCGCAACTTAGCCATCGCCTGACGGAATGTTTCATc         <  2:5173583/93‑1 (MQ=255)

                                                                                gTTATCGTTCGCATCACGTAACAGAACGCTCTGGTTCAGCAAAGTAACGCCTACCCGGCGCAACTTAGCCATCACCTGACGGAATGTTTCATc         <  1:6561900/93‑1 (MQ=255)

                                                                                 ttATCGTTCGCATCACGTAACAGAACGCTCTGGTTCAGCAAAGTAACGCCTACCCGGCGCAACTTAGCCATCGCCTGACGGAATGTTTCATCt        >  2:2611473/1‑93 (MQ=255)

                                                                                  tATCGTTCGCATCACGTAACAGAACGCTCTGGTTCAGCAAAGTAACGCCTACCCGGCGCAACTTAGCCATCGCCTGACGGAATGTTTCATCTa       >  1:1633935/1‑93 (MQ=255)

                                                                                    tCGTTCGCATCACGTAACAGAACGCTCTGGTTCAGCAAAGTAACGCCTACCCGGCGCAACTTAGCCATCGCCTGACGGAATGTTTCATCTAcc     <  2:2405134/93‑1 (MQ=255)

                                                                                    tCGTTCGCATCACGTAACAGAACGCTCTGGTTCAGCAAAGTAACGCCTACCCGGCGCAACTTAGCCATCGCCTGACGGAATGTTTCATCTAcc     <  1:5796638/93‑1 (MQ=255)

                                                                                    tCGTTCGCATCACGTAACAGAACGCTCTGGTTCAGCAAAGTAACGCCTACCCGGCGCAACTTAGCCATCGCCTGACGGAATGTTTCATCTAcc     <  1:4098958/93‑1 (MQ=255)

                                                                                     cGTTCGCATCACGTAACAGAACGCTCTGGTTCAGCAAAGTAACGCCTACCCGGCGCAACTTAGCCATCGCCTGACGGAATGTTTCATCTACCt    <  2:2155747/93‑1 (MQ=255)

                                                                                     cGTTCGCATCACGTAACAGAACGCTCTGGTTCAGCAAAGTAACGCCTACCCGGCGCAACTTAGCCATCGCCTGACGGAATGTTTCATCTACCt    >  1:5668591/1‑93 (MQ=255)

                                                                                      gTTCGCATCCCGTAACAGAGCGCTCTGGTTCAGCAAAGTAACGCCTACCCGGCGCAACTTAGCCATCGCCTGACGGAATGTTTCATCTACCTc   <  1:111949/93‑1 (MQ=255)

                                                                                      gTTCGCATCACGTAACAGAACGCTCTGGTTCAGCAAAGTAACGCCTACCCGGCGCAACTTAGCCATCGCCTGACGGAATGTTTCATCTACCTc   <  1:4286163/93‑1 (MQ=255)

                                                                                      gTTCGCATCACGTAACAGAACGCTCTGGTTCAGCAAAGTAACGCCTACCCGGCGCAACTTAGCCATCGCCTGACGGAATGTTTCATCTACCTc   >  2:1139398/1‑93 (MQ=255)

ACTTTATCGAGCACATGCAGGTAATACGGCATTACGCCGGCATCGAACAACGCATTACTCAGGTTTGCCAGCGTTTGTGCGTTATCGTTCACATCACGTAACAGAACGCTCTGGTTCAGCAAAGTAACGCCTACCCGGCGCAACTTAGCCATCGCCTGACGGAATGTTTCATCTACCTCA  >  NC_000913/4372367‑4372546

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 21 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`