BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,395,292	T→A	S101R (AGT→AGA)	mutL →	MutL

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,395,292	0	T	A	99.6%	905.4 / NA	246	S101R (AGT→AGA)	mutL	MutL
Reads supporting (aligned to +/- strand): ref base T (0/0); major base A (134/111); minor base C (1/0); total (135/111)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.98e-01

Only 100 of 256 total aligned reads displayed.

GCTGGCTCGTCATGCCACCAGTAAAATCGCCTCTCTGGACGATCTCGAAGCCATTATCAGCCTGGGCTTTCGCGGTGAGGCGCTGGCGAGTATCAGTTCGGTTTCCCGCCTGACGCTCACTTCACGCACCGCAGAACAGCAGGAAGCCTGGCAGGCCTATGCCGAAGGGCGCGATATGAA  >  NC_000913/4395202‑4395381

  tggcTCGTCATGCCACCAGTAAAATCGCCTCTCTGGACGATCTCGAAGCCATTATCAGCCTGGGCTTTCGCGGTGAGGCGCTGGCGAGAATCa                                                                                       >  2:3382931/1‑93 (MQ=255)

  tggcTCGTCATGCCACCAGTAAAATCGCCTCTCTGGACGATCTCGAAGCCATTATCAGCCTGGGCTTTCGCGGTGAGGCGCTGGCGAGAATCa                                                                                       >  1:3776981/1‑93 (MQ=255)

  tggcTCGTCATGCCACCAGTAAAATCGCCTCTCTGGACGATCTCGAAGCCATTATCAGCCTGGGCTTTCGCGGTGAGGCGCTGGCGAGAATCa                                                                                       >  2:5054015/1‑93 (MQ=255)

  tggcTCGTCATGCCACCAGTAAAATCGCCTCTCTGGACGATCTCGAAGCCATTATCAGCCTGGGCTTTCGCGGTGAGGCGCTGGCGAGAATCa                                                                                       <  1:201341/93‑1 (MQ=255)

   ggcTCGTCATGCCACCAGTAAAATCGCCTCTCTGGACGATCTCGAAGCCATTATCAGCCTGGGCTTTCGCGGTGAGGCGCTGGCGAGAATCAg                                                                                      <  1:5461034/93‑1 (MQ=255)

    gcTCGTCATGCCACCAGTAAAATCGCCTCTCTGGACGATCTCGAAGCCATTATCAGCCTGGGCTTTCGCGGTGAGGCGCTGGCGAGAATCAGt                                                                                     >  1:3238110/1‑93 (MQ=255)

    gcTCGTCATGCCACCAGTAAAATCGCCTCTCTGGACGATCTCGAAGCCATTATCAGCCTGGGCTTTCGCGGTGAGGCGCTGGCGAGAATCAGt                                                                                     <  1:4745270/93‑1 (MQ=255)

    gcTCGTCATGCCACCAGTAAAATCGCCTCTCTGGACGATCTCGAAGCCATTATCAGCCTGGGCTTTCGCGGTGAGGCGCTGGCGAGAATCAGt                                                                                     <  1:5478000/93‑1 (MQ=255)

     cTCGTCATGCCACCAGTAAAATCGCCTCTCTGGACGATCTCGAAGCCATTATCAGCCTGGGCTTTCGCGGTGAGGCGCTGGCGAGAATCAGtt                                                                                    <  2:5891536/93‑1 (MQ=255)

      tCGTCATGCCACCAGTAAAATCGCCTCTCTGGACGATCTCGAAGCCATTATCAGCCTGGGCTTTCGCGGTGAGGCGCTGGCGAGAATCAGTTc                                                                                   <  2:3002193/93‑1 (MQ=255)

       cGTCATGCCACCAGTAAAATCGCCTCTCTGGACGATCTCGAAGCCATTATCAGCCTGGGCTTTCGCGGTGAGGCGCTGGCGAGAATCAGTTCg                                                                                  >  1:383336/1‑93 (MQ=255)

        gTCATGCCACCAGTAAAATCGCCTCTCTGGACGATCTCGAAGCCATTATCAGCCTGGGCTTTCGCGGTGAGGCGCTGGCGAGAATCAGTTCgg                                                                                 >  1:5927462/1‑93 (MQ=255)

        ctcctGCCACCAGTAAAATCGCCTCTCTGGACGATCTCGAAGCCATTATCAGCCTGGGCTTTCGCGGTGAGGCGCTGGCGAGAATCAGTTCgg                                                                                 <  1:2861428/89‑1 (MQ=255)

         tCATGCCACCAGTAAAATCGCCTCTCTGGACGATCTCGAAGCCATTATCAGCCTGGGCTTTCGCGGTGAGGCGCTGGCGAGAATCAGTTCGGt                                                                                <  1:2318759/93‑1 (MQ=255)

         tCATGCCACCAGTAAAATCGCCTCTCTGGACGATCTCGAAGCCATTATCAGCCTGGGCTTTCGCGGTGAGGCGCTGGCGAGAATCAGTTCGGt                                                                                >  2:1851325/1‑93 (MQ=255)

         tCATGCCACCAGTAAAATCGCCTCTCTGGACGATCTCGAAGCCATTATCAGCCTGGGCTTTCGCGGTGAGGCGCTGGCGAGAATCAGTTCGGt                                                                                <  1:1263043/93‑1 (MQ=255)

           aTGCCACCAGTAAAATCGCCTCTCTGGACGATCTCGAAGCCATTATCAGCCTGGGCTTTCGCGGTGAGGCGCTGGCGAGAATCAGTTCGGttt                                                                              >  2:3535187/1‑93 (MQ=255)

            tGCCACCAGTAAAATCGCCTCTCTGGACGATCTCGAAGCCATTATCAGCCTGGGCTTTTGCGGTGAGGCGCTGGCGAGAATCACTTCGGTTTc                                                                             >  2:5348566/1‑93 (MQ=255)

             gccgccAGTAAAATCGCCTCTCTGGACGATCTCGAAGCCATTATCAGCCTGGGCTTTCGCGGTGAGGCGCTGGCGAGAATCAGTTCGGTTTcc                                                                            <  1:2467034/93‑1 (MQ=255)

             gCCACCAGTAAAATCGCCTCTCTGGACGATCTCGAAGCCATTATCAGCCTGGGCTTTCGCGGTGAGGCGCTGGCGAGAATCAGTTCGGTTTcc                                                                            <  2:1630616/93‑1 (MQ=255)

             gCCACCAGAAAAATCGCCTTTCTGGACGATCTCGAAGCCATTATCAGCCTGGGCTTTCGCGGTGAGGCGCTGGCGAGCATCAGTTCGGTTTcc                                                                            >  1:6528399/1‑93 (MQ=255)

               caccaGTAAAATCGCCTCTCTGGACGATCTCGAAGCCATTATCAGCCTGGGCTTTCGCGGTGAGGCGCTGGCGAGAATCAGTTCGGTTTCCCg                                                                          <  1:393726/93‑1 (MQ=255)

               caccaGTAAAATCGCCTCTCTGGACGATCTCGAAGCCATTATCAGCCTGGGCTTTCGCGGTGAGGCGCTGGCGAGAATCAGTTCGGTTTCCCg                                                                          >  1:5211508/1‑93 (MQ=255)

               caccaGTAAAATCGCCTCTCTGGACGATCTCGAAGCCATTATCAGCCTGGGCTTTCGCGGTGAGGCGCTGGCGAGAATCAGTTCGGTTTCCCg                                                                          <  1:4269887/93‑1 (MQ=255)

               caccaGTAAAATCGCCTCTCTGGACGATCTCGAAGCCATTATCAGCCTGGGCTTTCGCGGTGAGGCGCTGGCGAGAATCAGTTCGGTTTCCCg                                                                          <  1:2798816/93‑1 (MQ=255)

                 ccaGTAAAATCGCCTCTCTGGACGATCTCGAAGCCATTATCAGCCTGGGCTTTCGCGGTGAGGCGCTGGCGAGAATCAGTTCGGTTTCCCGcc                                                                        >  2:4687826/1‑93 (MQ=255)

                 ccaGTAAAATCGCCTCTCTGGACGATCTCGAAGCCATTATCAGCCTGGGCTTTCGCGGTGAGGCGCTGGCGAGAATCAGTTCGGTTTCCCGcc                                                                        >  2:5487768/1‑93 (MQ=255)

                 ccaGTAAAATCGCCTCTCTGGACGATCTCGAAGCCATTATCAGCCTGGGCTTTCGCGGTGAGGCGCTGGCGAGAATCAGTTCGGTTTCCCGcc                                                                        >  2:5551277/1‑93 (MQ=255)

                  caGTAAAATCGCCTCTCTGGACGATCTCGAAGCCATTATCAGCCTGGGCTTTCGCGGTGAGGCGCTGGCGAGAATCAGTTCGGTTTCCCGCCt                                                                       >  1:911879/1‑93 (MQ=255)

                  caGTAAAATCGCCTCTCTGGACGATCTCGAAGCCATTATCAGCCTGGGCTTTCGCGGTGAGGCGCTGGCGAGAATCAGTTCGGTTTCCCGCCt                                                                       >  1:6347319/1‑93 (MQ=255)

                  caGTAAAATCGCCTCTCTGGACGATCTCGAAGCCATTATCAGCCTGGGCTTTCGCGGTGAGGCGCTGGCGAGAATCAGTTCGGTTTCCCGCCt                                                                       >  2:5750660/1‑93 (MQ=255)

                   aGTAAAATCGCCTCTCTGGACGATCTCGAAGCCATTATCAGCCTGGGCTTTCGCGGTGAGGCGCTGGCGAGAATCAGTTCGGTTTCCCGCCTg                                                                      >  2:268240/1‑93 (MQ=255)

                      aaaaTCGCCTCTCTGGACGATCTCGAAGCCATTATCAGCCTGGGCTTTCGCGGTGAGGCGCTGGCGAGAATCAGTTCGGTTTCCCGCCTGACg                                                                   >  2:1184919/1‑93 (MQ=255)

                       aaaTCGCCTCTCTGGACGATCTCGAAGCCATTATCAGCCTGGGCTTTCGCGGTGAGGCGCTGGCGAGAATCAGTTCGGTTTCCCGCCTGACGc                                                                  >  1:4594943/1‑93 (MQ=255)

                       aaaTCGCCTCTCTGGACGATCTCGAAGCCATTATCAGCCTGGGCTTTCGCGGTGAGGCGCTGGCGAGAATCAGTTCGGTTTCCCGCCTGACGc                                                                  <  1:6668663/93‑1 (MQ=255)

                       aaaTCGCCTCTCTGGACGATCTCGAAGCCATTATCAGCCTGGGCTTTCGCGGTGAGGCGCTGGCGAGAATCAGTTCGGTTTCCCGCCTGACGc                                                                  >  1:1827840/1‑93 (MQ=255)

                        aaTCGCCTCTCTGGACGATCTCGAGGCCATTATCAGCCTGGGCTAAAGCGGTGAGGCGCTGGCGAGAATCAGTTCGGTTTCCCGCCTGACGCt                                                                 <  2:6726022/93‑1 (MQ=255)

                        aaTCGCCTCTCTGGACGATCTCGAAGCCATTATCAGCCTGGGCTTTCGCGGTGAGGCGCTGGCGAGAATCAGTTCGGTTTCCCGCCTGACGCt                                                                 >  1:6265893/1‑93 (MQ=255)

                         aTCGCCTCTCTGGACGATCTCGAAGCCATTATCAGCCTGGGCTTTCGCGGTGAGGCGCTGGCGAGAATCAGTTCGGTTTCCCGCCTGACGCTc                                                                >  2:380686/1‑93 (MQ=255)

                         aTCGCCTCTCTGGACGATCTCGAAGCCATTATCAGCCTGGGCTTTCGCGGTGAGGCGCTGGCGAGAATCAGTTCGGTTTCCCGCCTGACGCTc                                                                >  2:6474583/1‑93 (MQ=255)

                            gCCTCTCTGGACGATCTCGAAGCCATTATCAGCCTGGGCTTTCGCGGTGAGGCGCTGGCGAGAATCAGTTCGGTTTCCCGCCTGACGCTCACt                                                             >  1:4162154/1‑93 (MQ=255)

                            gCCTCTCTGGACGATCTCGAAGCCATTATCAGCCTGGGCTTTCGCGGTGAGGCGCTGGCGAGAATCAGTTCGGTTTCCCGCCTGACGCTCACt                                                             >  1:3883849/1‑93 (MQ=255)

                               tctctGGACGATCTCGAAGCCATTATCAGCCTGGGCTTTCGCGGTGAGGCGCTGGCGAGAATCAGTTCGGTTTCCCGCCTGACGCTCACTTCa                                                          >  2:3795137/1‑93 (MQ=255)

                                ctctGGACGATCTCGAAGCCATTATCAGCCTGGGCTTTCGCGGTGAGGCGCTGGCGAGAATCAGTTCGGTTTCCCGCCTGTCGCTCACTTCAc                                                         <  2:3591552/93‑1 (MQ=255)

                                 tctGGACGATCTCGAAGCCATTATCAGCCTGGGCTTTCGCGGTGAGGCGCTGGCGAGAATCAGTTCGGATTCCCGCCTGACGCTCACTTCACg                                                        >  2:3959301/1‑93 (MQ=255)

                                    ggtcGATCTCGAAGCCATGATCAGCCTGGGCTTTCGGGGTGAGGCGCTGGCGAGAATCAGTTCGGTTTCCCGCCTGACGCTCACTTCACGCAc                                                     <  2:3467980/90‑1 (MQ=255)

                                    ggACGATCTCGAAGCCATTATCAGCCTGGGCTTTCGCGGTGAGGCGCTGGCGAGAATCAGTTCGGTTTCCCGCCTGACGCTCACTTCACGCAc                                                     <  2:6135275/93‑1 (MQ=255)

                                          tctcGAAGCCATTATCAGCCTGGGCTTTCGCGGTGAGGCGCTGGCGAGAATCAGTTCGGTTTCCCGCCTGACGCTCACTTCACGCACCGCAGa                                               >  1:5711473/1‑93 (MQ=255)

                                          tctcGAAGCCATTATCAGCCTGGGCTTTCGCGGTGAGGCGCTGGCGAGAATCAGTTCGGTTTCCCGCCTGACGCTCACTTCACGCACCGCAGa                                               >  2:5695973/1‑93 (MQ=255)

                                            tcGAAGCCATTATCAGCCTGGGCTTTCGCGGTGAGGCGCTGGCGAGAATCAGTTCGGTTTCCCGCCTGACGCTCACTTCACGCACCGCAGAAc                                             >  2:216569/1‑93 (MQ=255)

                                             cGAAGCCATTATCAGCCTGGGCTTTCGCGGTGAGGCGCTGGCGAGAATCAGTTCGGTTTCCCGCCTGACGCTCACTTCACGCACCGCAGAAca                                            <  1:3436696/93‑1 (MQ=255)

                                              gAAGCCATTATCAGCCTGGGCTTTCGCGGTGAGGCGCTGGCGAGAATCAGTTCGGTTTCCCGCCTGACGCTCACTTCACGCACCGCAGAAcag                                           >  2:5448935/1‑93 (MQ=255)

                                               aaGCCATTATCAGCCTGGGCTTTCGCGGTGAGGCGCTGGCGAGAATCAGTTCGGTTTCCCGCCTGACGCTCACTTCACGCACCGCAGAAcagc                                          >  2:1753865/1‑93 (MQ=255)

                                                aGCCATTATCAGCCTGGGCTTTCGCGGTGAGGCGCTGGCGAGAATCAGTTCGGTTTCCCGCCTGACGCTCACTTCACGCACCGCAGAAcagca                                         <  2:6678945/93‑1 (MQ=255)

                                                aGCCATTATCAGCCTGGGCTTTCGCGGTGAGGCGCTGGCGAGAATCAGTTCGGTTTCCCGCCTGACGCTCACTTCACGCACCGCAGAAcagca                                         <  2:2075558/93‑1 (MQ=255)

                                                 gCCATTATCAGCCTGGGCTTTCGCGGTGAGGCGCTGGCGAGAATCAGTTCGGTTTCCCGCCTGACGCTCACTTCACGCACCGCAGAAcagcag                                        <  1:5295713/93‑1 (MQ=255)

                                                  ccATTATCAGCCTGGGCTTTCGAGGTGAGGCGCTGGCGAGAATCAGTTCGGTTTCCCGCCTGACGCTCACTTCACGCACCGCAGAACAGCAgg                                       <  2:2292659/93‑1 (MQ=255)

                                                   cATTATCAGCCTGGGCTTTCGCGGTGAGGCGCTGGCGAGAATCAGTTCGGTTTCCCGCCTGACGCTCACTTCACGCACCGCAGAACAGCAGGa                                      >  1:6273434/1‑93 (MQ=255)

                                                   cATTATCAGCCTGGGCTTTCGCGGTGAGGCGCTGGCGAGAATCAGTTCGGTTTCCCGCCTGACGCTCACTTCACGCACCGCAGAACAGCAGGa                                      <  1:396692/93‑1 (MQ=255)

                                                   cATTATCAGCCTGGGCTTTCGCGGTGAGGCGCTGGCGAGAATCAGTTCGGTTTCCCGCCTGACGCTCACTTCACGCACCGCAGAACAGCAGGa                                      <  1:5590683/93‑1 (MQ=255)

                                                    aTTATCAGCCTGGGCTTTCGCGGTGAGGCGCTGGCGAGAATCAGTTCGGTTTCCCGCCTGACGCTCACTTCACGCACCGCAGAACAGCAGGaa                                     <  1:3538221/93‑1 (MQ=255)

                                                    aTTATCAGCCTGGGCTTTCGCGGTGAGGCGCTGGCGAGAATCAGTTCGGTTTCCCGCCTGACGCTCACTTCACGCACCGCAGAACAGCAGGaa                                     >  2:2838319/1‑93 (MQ=255)

                                                    aTTATCAGCCTGGGCTTTCGCGGTGAGGCGCTGGCGAGAATCAGTTCGGTTTCCCGCCTGACGCTCACTTCACGCACCGCAGAACAGCAGGaa                                     <  1:6053494/93‑1 (MQ=255)

                                                    aTTATCAGCCTGGGCTTTCGCGGTGAGGCGCTGGCGAGAATCAGTTCGGTTTCCCGCCTGACGCTCACTTCACGCACCGCAGAACAGCAGGaa                                     >  1:4442902/1‑93 (MQ=255)

                                                    aTTATCAGCCTGGGCTTTCGCGGTGAGGCGCTGGCGAGAATCAGTTCGGTTTCCCGCCTGACGCTCACTTCACGCACCGCAGAACAGCAGGaa                                     <  2:1552033/93‑1 (MQ=255)

                                                    aTTATCAGCCTGGGCTTTCGCGGTGAGGCGCTGGCGAGAATCAGTTCGGTTTCCCGCCTGACGCTCACTTCACGCACCGCAGAACAGCAGGaa                                     <  1:5054015/93‑1 (MQ=255)

                                                     ttATCAGCCTGGGCTTTCGCGGTGAGGCGCTGGCGAGAATCAGTTCGGTTTCCCGCCTGACGCTCACTTCACGCACCGCAGAACAGCAGGAAg                                    <  1:5887394/93‑1 (MQ=255)

                                                       aTCAGCCTGGGCTTTCGCGGTGAGGCGCTGGCGAGAATCAGTTCGGTTTCCCGCCTGACGCTCACTTCACGCACCGCAGAACAGCAGGAAGcc                                  <  1:1003505/93‑1 (MQ=255)

                                                        tCAGCCTGGGCTTTCGCGGTGAGGCGCTGGCGAGAATCAGTTCGGTTTCCCGCCTGACGCTCACTTCACGCACCGCAGAACAGCAGGAAGCCt                                 >  1:2633349/1‑93 (MQ=255)

                                                        tCAGCCTGGGCTTTCGCGGTGAGGCGCTGGCGAGAATCAGTTCGGTTTCCCGCCTGACGCTCACTTCACGCACCGCAGAACAGCAGGAAGCCt                                 >  2:3137065/1‑93 (MQ=255)

                                                           gCCTGGGCTTTCGCGGTGAGGCGCTGGCGAGAATCAGTTCGGTTTCCCGCCTGACGCTCACTTCACGCACCGCAGAACAGCAGGAAGCCTGGc                              >  2:1511297/1‑93 (MQ=255)

                                                            ccTGGGCTTTCGCGGTGAGGCGCTGGCGAGAATCAGTTCGGTTTCCCGCCTGACGCTCACTTCACGCACCGCAGAACAGCAGGAAGCCTGGCa                             <  2:407636/93‑1 (MQ=255)

                                                            ccTGGGCTTTCGCGGTGAGGCGCTGGCGAGAATCAGTTCGGTTTCCCGCCTGACGCTCACTTCACGCACCGCAGAACAGCAGGAAGCCTGGCa                             <  2:778036/93‑1 (MQ=255)

                                                             cTGGGCTTTCGCGGTGAGGCGCTGGCGAGAATCAGTTCGGTTTCCCGCCTGACGCTCACTTCACGCACCGCAGAACAGCAGGAAGCCTGGCAg                            >  1:3868058/1‑93 (MQ=255)

                                                              tGGGCTTTCGCGGTGAGGCGCTGGCGAGAATCAGTTCGGTTTCCCGCCTGACGCTCACTTCACGCACCGCAGAACAGCAGGAAGCCTGGCAgg                           >  2:3053908/1‑93 (MQ=255)

                                                               gggCTTTCGCGGTGAGGCGCTGGCGAGAATCAGTTCGGTTTCCCGCCTGACGCTCACTTCACGCACCGCAGAACAGCAGGAAGCCTGGCAGGc                          >  2:2106487/1‑93 (MQ=255)

                                                                 gCTTTCGCGGTGAGGCGCTGGCGAGAATCAGTTCGGTTTCCCGCCTGACGCTCACTTCACGCACCGCAGAACAGCAGGAAGCCTGGCAGGCCt                        <  2:2912219/93‑1 (MQ=255)

                                                                  cTTTCGCGGTGAGGCGCTGGCGAGAATCAGTTCGGTTTCCCGCCTGACGCTCACTTCACGCACCGCAGAACAGCAGGAAGCCTGGCAGGCCTa                       >  2:4905326/1‑93 (MQ=255)

                                                                     tCGCGGTGAGGCGCTGGCGAGAATCAGTTCGGTTTCCCGCCTGACGCTCACTTCACGCACCGCAGAACAGCAGGAAGCCTCGCAGGCCTATGc                    >  2:4405929/1‑93 (MQ=255)

                                                                     ccgcgGTGAGGCGCGGGCGAGAACCAGTTCGGTTTCCCGCCTGACGCTCACTTCACGCACCGCAGAACAGCAGGAAGCCTGGCAGGCCTATGc                    <  1:1176796/92‑1 (MQ=255)

                                                                      cgcgGTGAGGCGCTGGCGAGAATCAGTTCGGTTTCCCGCCTGACGCTCACTTCACGCACCGCAGAACAGCAGGAAGCCTGGCAGGCCTATGcc                   >  1:3072678/1‑93 (MQ=255)

                                                                       gcgGTGAGGCGCTGGCGAGAATCAGTTCGGTTTCCCGCCTGACGCTCACTTCACGCACCGCAGAACAGCAGGAAGCCTGGCAGGCCTATGCCg                  <  1:1467558/93‑1 (MQ=255)

                                                                        cgGTGAGGCGCTGGCGAGAATCAGTTCGGTTTCCCGCCTGACGCTCACTTCACGCACCGCAGAACAGCAGGAAGCCTGGCAGGCCTATGCCGa                 >  1:4297766/1‑93 (MQ=255)

                                                                        cgGTGAGGCGCTGGAGAGAATCAGTTCGGTTTCCCGCCTGACGCTCACTTCACGCACCGCAGAACAGCAGGAAGCCTGGCAGGCCTATGCCGa                 >  1:988687/1‑93 (MQ=255)

                                                                         ggTGAGGCGCTGGCGAGAATCAGTTCGGTTTCCCGCCTGACGCTCACTTCACGCACCGCAGAACAGCAGGAAGCCTGGCAGGCCTATGCCGaa                >  1:784542/1‑93 (MQ=255)

                                                                          gTGAGGCGCTGGCGAGAATCAGTTCGGTTTCCCGCCTGACGCTCACTTCACGCACCGCAGAACAGCAGGAAGCCTGGCAGGCCTATGCCGAAg               <  2:1331520/93‑1 (MQ=255)

                                                                          gTGAGGCGCTGGCGAGAATCAGTTCGGTTTCCCGCCTGACGCTCACTTCACGCACCGCAGAACAGCAGGAAGCCTGGCAGGCCTATGCCGAAg               >  2:6529529/1‑93 (MQ=255)

                                                                           tGAGGCGCTGGCGAGAATCAGTTCGGTTTCCCGCCTGACGCTCACTTCACGCACCGCAGAACAGCAGGAAGCCTGGCAGGCCTATGCCGAAgg              <  2:6323908/93‑1 (MQ=255)

                                                                            gAGGCGCTGGCGAGAATCAGTTCGGTTTCCCGCCTGACGCTCACTTCACGCACCGCAGAACAGCAGGAAGCCTGGCAGGCCTATGCCGAAggg             >  1:1103111/1‑93 (MQ=255)

                                                                            gAGGCGCTGGCGAGAATCAGTTCGGTTTCCCGCCTGACGCTCACTTCACGCACCGCAGAACAGCAGGAAGCCTGGCAGGCCTATGCCGAAggg             <  2:2571252/93‑1 (MQ=255)

                                                                             aGGCGCTGGCGAGAATCAGTTCGGTTTCCCGCCTGACGCTCACTTCACGCACCGCAGAACAGCAGGAAGCCTGGCAGGCCTATGCCGAAGGgc            <  1:1401635/93‑1 (MQ=255)

                                                                              ggCGCTGGCGAGAATCAGTTCGGTTTCCCGCCTGACGCTCACTTCACGCACCGCAGAACAGCAGGAAGCCTGGCAGGCCTATGCCGAAGGgcg           <  2:592596/93‑1 (MQ=255)

                                                                               gcgcTGGCGAGAATCAGTTCGGTTTCCCGCCTGACGCTCACTTCACGCACCGCAGAACAGCAGGAAGCCTGGCAGGCCTATGCCGGAGGgcgc          >  2:5984490/1‑93 (MQ=255)

                                                                                cgcTGGCGAGAATCAGTTCGGTTTCCCGCCTGACGCTCACTTCACGCACCGCAGAACAGCAGGAAGCCTGGCAGGCCTATGCCGAAGGGcgcg         >  1:2924680/1‑93 (MQ=255)

                                                                                   tGGCGAGAATCAGTTCGGTTTCCCGCCTGACGCTCACTTCACGCACCGCAGAACAGCAGGAAGCCTGGCAGGCCTATGCCGAAGGGCGCGata      >  1:4965250/1‑93 (MQ=255)

                                                                                   tGGCGAGAATCAGTTCGGTTTCCCGCCTGACGCTCACTTCACGCACCGCAGAACAGCAGGAAGCCTGGCAGGCCTATGCCGAAGGGCGCGaca      <  1:6620060/93‑3 (MQ=255)

                                                                                    ggCGAGAATCAGTTCGGTTTCCCGCCTGACGCTCACTTCACGCACCGCAGAACAGCAGGAAGCCTGGCAGGCCTATGCCGAAGGGCGCGatat     <  2:5460428/93‑1 (MQ=255)

                                                                                    ggCGAGAATCAGTTCGGTTTCCCGCCTGACGCTCACTTCACGCACCGCAGAACAGCAGGAAGCCTGGCAGGCCTATGCCGAAGGGCGCGatat     <  2:3258049/93‑1 (MQ=255)

                                                                                       gagaATCAGTTCGGTTTCCCGCCTGACGCTCACTTCACGCACCGCAGAACAGCAGGAAGCCTGGCAGGCCTATGCCGAAGGGCGCGATATGaa  <  1:4705364/93‑1 (MQ=255)

                                                                                       gagaATCAGTTCGGTTTCCCGCCTGACGCTCACTTCACGCACCGCAGAACAGCAGGAAGCCTGGCAGGCCTATGCCGAAGGGCGCGATATGaa  <  1:4854963/93‑1 (MQ=255)

GCTGGCTCGTCATGCCACCAGTAAAATCGCCTCTCTGGACGATCTCGAAGCCATTATCAGCCTGGGCTTTCGCGGTGAGGCGCTGGCGAGTATCAGTTCGGTTTCCCGCCTGACGCTCACTTCACGCACCGCAGAACAGCAGGAAGCCTGGCAGGCCTATGCCGAAGGGCGCGATATGAA  >  NC_000913/4395202‑4395381

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 19 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`