BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,431,142	T→C	H154R (CAC→CGC)	ytfG ←	putative oxidoreductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,431,142	0	T	C	100.0%	914.1 / NA	284	H154R (CAC→CGC)	ytfG	putative oxidoreductase
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (134/150); total (134/150)

Only 100 of 303 total aligned reads displayed.

TCGCTAATCACGCGTGCCGCAGCTGCCGCATAATCTGCCCGCGTTGCTGAGGCGATTTTGCCATCGCCCGCCGCACCGATAAATACGCCGTGTTCCAGTGCTGCCGGGGCGCTGGCGAGGTAGTTTTCGCTGTACCAGCCGTTGCGCAGCAGGGTGTAAACGATGCCAGAATCAGCCAGC  >  NC_000913/4431052‑4431231

tCGCTAATCACGCGTGCCGCAGCTGCCGCATAATCTGCCCGCGTTGCTGAGGCGATTTTGCCATCGCCCGCCGCACCGATAAATACGCCgcgt                                                                                         <  1:5092749/93‑1 (MQ=255)

tCGCTAATCACGCGTGCCGCAGCTGCCGCATAATCTGCCCGCGTTGCTGAGGCGATTTTGCCATCGCCCGCCGCACCGATAAATACGCCgcgt                                                                                         <  1:3822091/93‑1 (MQ=255)

   cTAATCACGCGTGCCGCAGCTGCCGCATAATCTGCCCGCGTTGCTGAGGCGATTTTGCCATCGCCCGCCGCACCGATAAATACGCCGCGTTcc                                                                                      >  2:2730761/1‑93 (MQ=255)

    tAATCACGCGTGCCGCAGCTGCCGCATAATCTGCCCGCGTTGCTGAGGCGATTTTGCCATCGCCCGCCGCACCGATAAATACGCCGCGTTCCa                                                                                     >  1:6228526/1‑93 (MQ=255)

    tAATCACGCGTGCCGCAGCTGCCGCATAATCTGCCCGCGTTGCTGAGGCGATTTTGCCATCGCCCGCCGCACCGATAAATACGCCGCGTTCCa                                                                                     <  2:4765000/93‑1 (MQ=255)

     aaTCACGCGTGCCGCAGCTGCCGCATAATCTGCCCGCGTTGCTGAGGCGATTTTGCCATCGCCCGCCGCACCGATAAATACGCCGCGTTCCAg                                                                                    <  1:466011/93‑1 (MQ=255)

      aTCACGCGTGCCGCAGCTGCCGCATAATCTGCCCGCGTTGCTGAGGCGATTTTGCCATCGCCCGCCGCACCGATAAATACGCCGCGTTCCAgg                                                                                   >  1:2622195/1‑92 (MQ=255)

        cACGCGTGCCGCAGCTGCCGCATAATCTGCCCGCGTTGCTGAGGCGATTTTGCCATCGCCCGCCGCACCGATAAATACGCCGCGTTCCAGtgc                                                                                 <  1:3483577/93‑1 (MQ=255)

         aCGCGTGCCGCAGCTGCCGCATAATCTGCCCGCGTTGCTGAGGCGATTTTGCCATCGCCCGCCGCACCGATAAATACGCCGCGTTCCAGtgct                                                                                >  1:6316603/1‑93 (MQ=255)

           gcgTGCCGCAGCTGCCGCATAATCTGCCCGCGTTGCTGAGGCGATTTTGCCATCGCCCGCCGCACCGATAAATACGCCGCGTTCCAGtgctgc                                                                              >  2:6570172/1‑93 (MQ=255)

             gTGCCGGAGCTGCCGCATAATCTGCCCGCGTTGCTGAGGCGATTTTGCCATCGCCCGCCGCACCGATAAATACGCCGCGTTCCAGTGCTGCCg                                                                            <  2:717790/93‑1 (MQ=255)

              tGCCGCAGCTGTCGCATAATCTGCCCGCGTTGCTGAGGCGATTTTCCCATCGCCCGCCGCACCGATAAATACGCCGCGTTCCAGTGCTGCCgg                                                                           <  1:809527/93‑1 (MQ=255)

              tGCCGCAGCTGCCGCATAATCTGCCCGCGTTGCTGAGGCGATTTTGCCATCGCCCGCCGCACCGATAAATACGCCGCGTTCCAGTGCCGCCgg                                                                           <  1:4340697/93‑1 (MQ=255)

               gCCGCAGCTGCCGCATAATCTGCCCGCGTTGCTGAGGCGATTTTGCCATCGCCCGCCGCACCGATAAATACGCCGCGTTCCAGTGCTGCCggg                                                                          <  2:4531663/93‑1 (MQ=255)

                 cGCAGCTGCCGCATAATCTGCCCGCGTTGCTGAGGCGATTTTGCCATCGCCCGCCGCACCGATAAATACGCCGCGTTCCAGTGCTGCCGGGgc                                                                        >  2:849536/1‑93 (MQ=255)

                 cGCAGCTGCCGCATAATCTGCCCGCGTTGCTGAGGCGATTTTGCCATCGCCCGCCGCACCGATAAATACGCCGCGTTCCAGTGCTGCCGGGgc                                                                        >  1:5187926/1‑93 (MQ=255)

                  gCAGCTGCCGCATAATCTGCCCGCGTTGCTGAGGCGATTTTGCCATCGCCCGCCGCACCGATAAATACGCCGCGTTCCAGTGCTGCCGGGgcg                                                                       >  1:4367925/1‑93 (MQ=255)

                   cAGCTGCCGCATAATCTGCCCGCGTTGCTGAGGCGATTTTGCCATCGCCCGCCGCACCGATAAATACGCCGCGTTCCAGTGCTGCCGGGgcgc                                                                      <  2:2568801/93‑1 (MQ=255)

                   cAGCTGCCGCATAATCTGCCCGCGTTGCTGAGGCGATTTTGCCATCGCCCGCCGCACCGATAAATACGCCGCGTTCCAGTGCTGCCGGGgcgc                                                                      <  1:1751471/93‑1 (MQ=255)

                   cAGCTGCCGCATAATCTGCCCGCGTTGCTGAGGCGATTTTGCCATCGCCCGCCGCACCGATAAATACGCCGCGTTCCAGTGCTGCCGGGgcgc                                                                      <  1:4093090/93‑1 (MQ=255)

                   cAGCTGCCGCATAATCTGCCCGCGTTGCTGAGGCGATTTTGCCATCGCCCGCCGCACCGATAAATACGCCGCGTTCCAGTGCTGCCGGGgcgc                                                                      <  2:3962618/93‑1 (MQ=255)

                    aGCTGCCGCATAATCTGCCCGCGTTGCTGAGGCGATTTTGCCATGGCCCGCCGCACCGATAAATACGCCGCGTTCCAGTGCTGCCGGGGCGCt                                                                     <  1:3209882/93‑1 (MQ=255)

                    aGCTGCCGCATAATCTGCCCGCGTTGCTGAGGCGATTTTGCCATCGCCCGCCGCACCGATAAATACGCCGCGTTCCAGTGCTGCCGGGGCGCt                                                                     >  2:2082553/1‑93 (MQ=255)

                    aGCTGCCGCATAATCTGCCCGCGTTGCTGAGGCGATTTTGCCATCGCCCGCCGCACCGATAAATACGCCGCGTTCCAGTGCTGCCGGGGCGCt                                                                     <  1:926088/93‑1 (MQ=255)

                       tGCCGCATAATCTGCCCGCGTTGCTGAGGCGATTTTGCCATCGCCCGCCGCACCGATAAATACGCCGCGTTCCAGTGCTGCCGGGGCGCTGGc                                                                  >  2:3892653/1‑93 (MQ=255)

                           gCATAATCTGCCCGCGTTGCTGAGGCGATTTTGCCATCGCCCGCCGCACCGATAAATACGCCGCGTTCCAGTGCTGCCGGGGCGCTGGCGAgg                                                              >  1:4874151/1‑93 (MQ=255)

                           gCATAATCTGCCCGCGTTGCTGAGGCGATTTTGCCATCGCCCGCCGCACCGATAAATACGCCGCGTTCCAGTGCTGCCGGGGCGCTGGCGAgg                                                              <  1:3409379/93‑1 (MQ=255)

                           gCATAATCTGCCCGCGTTGCTGAGGCGATTTTGCCATCGCCCGCCGCACCGATAAATACGCCGCGTTCCAGTGCTGCCGGGGAGCTGGCGAgg                                                              >  2:1243409/1‑93 (MQ=255)

                            cATAATCTGCCCGCGTTGCTGAGGCGATTTTGCCATCGCCCGCCGCACCGATAAATACGCCGCGTTCCAGTGCTGCCGGGGCGCTGGCGAGGt                                                             >  1:901828/1‑93 (MQ=255)

                            cATAATCTGCCCGCGTTGCTGAGGCGATTTTGCCATCGCCCGCCGCACCGATAAATACGCCGCGTTCCAGTGCTGCCGGGGCGCTGGCGAGGt                                                             <  1:4418473/93‑1 (MQ=255)

                            cATAATCTGCCCGCGTTGCTGAGGCGATTTTGCCATCGCCCGCCGCACCGATAAATACGCCGCGTTCCAGTGCTGCCGGGGCGCTGGCGAGGt                                                             <  2:5159024/93‑1 (MQ=255)

                            cATAATCTGCCCGCGTTGCTGAGGCGATTTTGCCATCGCCCGCCGCACCGATAAATACGCCGCGTTCCAGTGCTGCCGGGGCGCTGGCGAGGt                                                             >  2:6295673/1‑93 (MQ=255)

                            cATAATCTCCCCGCGTGCGTGAGGCGATTTTGCCCTCGCCCGCCGCACCGATAAATACGCCGCGTTCCAGTGCTGCCGGGGCGCTGGCGAGGt                                                             <  1:3953275/93‑1 (MQ=255)

                             aTAATCTGCCCGCGTTGCTGAGGCGATTTTGCCATCGCCCGCCGCACCGATAAATACGCCGCGTTCCAGTGCTGCCGGGGCGCTGGCGAGGTa                                                            <  2:317710/93‑1 (MQ=255)

                             aTAATCTGCCCGCGTTGCTGAGGCGATTTTGCCATCGCCCGCCGCACCGATAAATACGCCGCGTTCCAGTGCTGCCGGGGCGCTGGCGAGGTa                                                            >  1:1206716/1‑93 (MQ=255)

                             aTAATCTGCCCGCGTTGCTGAGGCGATTTTGCCATCGCCCGCCGCACCGATAAATACGCCGCGTTCCAGTGCTGCCGGGGCGCTGGCGAGGTa                                                            >  1:479488/1‑93 (MQ=255)

                              tAATCTGCCCGCGTTGCTGAGGCGATTTTGCCATCGCCCGCCGCACCGATAAATACGCCGCGTTCCAGTGCTGCCGGGGCGCTGGCGAGGTAg                                                           >  2:6167261/1‑93 (MQ=255)

                                aTCTGCCCGCGTTGCTGAGGCGATTTTGCCATCGCCCGCCGCACCGATAAATACGCCGCGTTCCAGTGCTGCCGGGGCGCTGGCGAGGTAGtt                                                         <  2:1925983/93‑1 (MQ=255)

                                  cTGCCCGCGTTGCTGAGGCGATTTTGCCATCGCCCGCCGCACCGATAAATACGCCGCGTTCCAGTGCTGCCGGGGCGCTGGCGAGGTAGtttt                                                       >  1:5576146/1‑93 (MQ=255)

                                    gCCCGCTTTGCTGCGGCGATTTTGCAATCGCCCGCCGCACCGATAAATACGCCGCGTTCCAGTGCTGCCGGGGCGCTGGCGAGGTAGTTTTCg                                                     <  1:635147/93‑1 (MQ=255)

                                    gCCCGCGTTGCTGAGGCGATTTTGCCATCGCCCGCCGCACCGATAAATACGCCGCGTTCCAGTGCTGCCGGGGCGCTGGCGAGGTAGTTTTCg                                                     <  2:1511517/93‑1 (MQ=255)

                                     cccGCGTTGCTGAGGCGATTTTGCCATCGCCCGCCGCACCGATAAATACGCCGCGTTCCAGTGCTGCCGGGGCGCTGGCGAGGTAGTTTTCGc                                                    >  1:4996348/1‑93 (MQ=255)

                                       cgcgTTGCTGAGGCGATTTTGCCATCGCCCGCCGCACCGATAAATACGCCGCGTTCCAGTGCTGCCGGGGCGCTGGCGAGGTAGTTTTCGCTg                                                  <  1:5486706/93‑1 (MQ=255)

                                        gcgTTGCTGAGGCGATTTTGCCATCGCCCGCCGCACCGATAAATACGCCGCGTTCCAGTGCTGCCGGGGCGCTGGCGAGGTAGTTTTCGCTGt                                                 <  1:1044163/93‑1 (MQ=255)

                                        gcgTTGCTGAGGCGATTTTGCCATCGCCCGCCGCACCGATAAATACGCCGCGTTCCAGTGCTGCCGGGGCGCTGGCGAGGTAGTTTTCGCTGt                                                 >  2:2695856/1‑93 (MQ=255)

                                        gcgTTGCTGAGGCGATTTTGCCATCGCCCGCCGCACCGATAAATACGCCGCGTTCCAGTGCTGCCGGGGCGCTGGCGAGGTAGTTTTCGCTGt                                                 >  2:1577943/1‑93 (MQ=255)

                                        gcgTTGCTGAGGCGATTTTGCCATCGCCCGCCGCACCGATAAATACGCCGCGTTCCAGTGCTGCCGGGGCGCTGGCGAGGTAGTTTTCGCTGt                                                 <  1:4517788/93‑1 (MQ=255)

                                        gcgTGGCTGAGGCGATTTTGCCATCGCCCGCCGCACCGATAAATACGCCGCGTTCCAGTGCTGCCGGGGCGCTGGCGAGGTAGTTTTCGCTGt                                                 <  1:1647447/93‑1 (MQ=255)

                                          gTTGCTGAGGCGATTTTGCCATCGCCCGCCGCACCGATAAATACGCCGCGTTCCAGTGCTGCCGGGGCGCTGGCGAGGTAGTTTTCGCTGTAc                                               >  2:3396521/1‑93 (MQ=255)

                                            tGCTGAGGCGATTTTGCCATCGCCCGCCGCACCGATAAATACGCCGCGTTCCAGTGCTGCCGGGGCGCTGGCGAGGTAGTTTTCGCTGTACCa                                             >  2:3580462/1‑93 (MQ=255)

                                              cTGAGGCGATTTTGCCATCGCCCGCCGCACCGATAAATACGCCGCGTTCCAGTGCTGCCGGGGCGCTGGCGAGGTAGTTTTCGCTGTACCAGc                                           >  2:6195411/1‑93 (MQ=255)

                                               tGAGGCGATTTTGCCATCGCCCGCCGCACCGATAAATACGCCGCGTTCCAGTGCTGCCGGGGCGCTGGCGAGGTAGTTTTCGCTGTACCAGcc                                          >  2:4303656/1‑93 (MQ=255)

                                               tGAGGCGATTGTGCAATCGCCCGCCGCAGCGATAAATACGCCGCGTTCCAGTGCTGCCGGGGCGCTGGCGAGGTAGTTTTCGCTGTACCAGcc                                          <  2:3019906/93‑1 (MQ=255)

                                                gAGGCGATTTTGCCATCGCCCGCCGCACCGATAAATACGCCGCGTTCCAGTGCTGCCGGGGCGCTGGCGAGGTAGTTTTCGCTGTACCAGCCg                                         >  2:3051168/1‑93 (MQ=255)

                                                 aGGCGATTTTGCCATCGCCCGCCGCACCGATAAATACGCCGCGTTCCAGTGCTGCCGGGGCGCTGGCGAGGTAGTTTTCGCTGTACCAGCCGt                                        >  2:5761900/1‑93 (MQ=255)

                                                  ggCGATTTTGCCATCGCCCGCCGCACCGATAAATACGCCGCGTTCCAGTGCTGCCGGGGCGCTGGCGAGGTAGTTTTCGCTGTACCAGCCGtt                                       <  1:1676711/93‑1 (MQ=255)

                                                  ggCGATTTTGCCATCGCCCGCCGCACCGATAAATACGCCGCGTTCCAGTGCTGCCGGGGCGCTGGCGAGGTAGTTTTCGCTGTACCAGCCGtt                                       >  1:240182/1‑93 (MQ=255)

                                                     gATTTTGCCATCGCCCGCCGCACCGATAAATACGCCGCGTTCCAGTGCTGCCGGGGCGCTGGCGAGGTAGTTTTCGCTGTACCAGCCGTTgcg                                    >  2:2344323/1‑93 (MQ=255)

                                                       ttttGCCATCGCCCGCCGCACCGATAAATACGCCGCGTTCCAGTGCTGCCGGGGCGCTGGCGAGGTAGTTTTCGCTGTACCAGCCGTTGCgca                                  >  2:6209504/1‑93 (MQ=255)

                                                       ttttGCCATCGCCCGCCGCACCGATAAATACGCCGCGTTCCAGTGCTGCCGGGGCGCTGGCGAGGTAGTTTTCGCTGTACCAGCCGTTGCgca                                  >  2:2469255/1‑93 (MQ=255)

                                                         ttGCCATCGCCCGCCGCACCGATAAATCCGCCGCGTTCCAGTGCTGCCGGGGCGCTGGCGAGGTAGTTTTCGCTGTACCAGCCGTTGCgcagc                                <  1:5675863/93‑1 (MQ=255)

                                                         ttGCCATCGCCCGCCGCACCGATAAATACGCCGCGTTCCAGTGCTGCCGGGGCGCTGGCGAGGTAGTTTTCGCTGTACCAGCCGTTGCgcagc                                <  2:1018228/93‑1 (MQ=255)

                                                          tGCCATCGCCCGCCGCACCGATAAATACGCCGCGTTCCAGTGCTGCCGGGGCGCTGGCGAGGTAGTTTTCGCTGTACCAGCCGTTGCgcagca                               <  2:6663556/93‑1 (MQ=255)

                                                          tGCCATCCCCCGCCGCACCGATAAATACGCCGCGTTCCAGTGCTGCCGGGGCGCTGGCGAGGTAGTTTTCGCTGTACCAGCCGTTGCgcagca                               <  1:5966563/93‑1 (MQ=255)

                                                           gCCATCGCCCGCCGCACCGATAAATACGCCGCGTTCCAGTGCTGCCGGGGCGCTGGCGAGGTAGTTTTCGCTGTACCAGCCGTTGCGcagcag                              <  2:3825500/93‑1 (MQ=255)

                                                             cATCGCCCGCCGCACCGATAAATACGCCGCGTTCCAGTGCTGCCGGGGCGCTGGCGAGGTAGTTTTCGCTGTACCAGCCGTTGCGCAGCAggg                            <  2:5532787/93‑1 (MQ=255)

                                                             cATCGCCCGCCGCACCGATAAATACGCCGCGTTCCAGTGCTGCCGGGGCGCTGGCGAGGTAGTTTTCGCTGTACCAGCCGTTGCGCAGCAggg                            >  1:5439055/1‑93 (MQ=255)

                                                             cATCGCCCGCCGCACCGATAAATACGCCGCGTTCCAGTGCTGCCGGGGCGCTGGCGAGGTAGTTTTCGCTGTACCAGCCGTTGCGCAGCAggg                            >  2:2603354/1‑93 (MQ=255)

                                                              ctCGCCCGCCGCACCGATAAATACGCCGCGTTCCAGTGCTGCCGGGGCGCTGGCGAGGTAGTTTTCGCTGTACCAGCCGTTGCGCAGCAGGgt                           <  1:146003/92‑1 (MQ=255)

                                                              ctCGCCCGCCGCACCGATAAATACGCCGCGTTCCAGTGCTGCCGGGGCGCTGGCGAGGTAGTTTTCGCTGTACCAGCCGTTGCGCAGCAGGgt                           <  1:4009596/92‑1 (MQ=255)

                                                               tcccccgccGCACCGATAAATACGCCGCGTTCCAGTGCTGCCGGGGCGCTGGCGAGGTAGTTTTCGCTGTACCAGCCGTTGCGCAGCAGGgtg                          <  1:3096508/90‑1 (MQ=255)

                                                                  cccgccGCACCGATAAATACGCCGCGTTCCAGTGCTGCCGGGGCGCTGGCGAGGTAGTTTTCGCTGTACCAGCCGTTGCGCAGCAGGGTGTaa                       >  2:781268/1‑93 (MQ=255)

                                                                   ccgccgCACCGATAAATACGCCGCGTTCCAGTGCTGCCGGGGCGCTGGCGAGGTAGTTTTCGCTGTACCAGCCGTTGCGCAGCAGGGTGTaaa                      <  1:2211779/93‑1 (MQ=255)

                                                                    cgccgcACCGATAAATACGCCGCGTTCCAGTGCTGCCGGGGCGCTGGCGAGGTAGTTTTCGCTGTACCAGCCGTTGCGCAGCAGGGTGTAAAc                     >  1:3330510/1‑93 (MQ=255)

                                                                      ccgcACCGATAAATACGCCGCGTTCCAGTGCTGCCGGGGCGCTGGCGAGGTAGTTTTCGCTGTACCAGCCGTTGCGCAGCAGGGTGTAAACGa                   <  2:1747065/93‑1 (MQ=255)

                                                                       cgcACCGATAAATACGCCGCGTTCCAGTGCTGCCGGGGCGCTGGCGAGGTAGTTTTCGCTGTACCAGCCGTTGCGCAGCAGGGTGTAAACGAt                  <  1:2740134/93‑1 (MQ=255)

                                                                       cgcACCGATAAATACGCCGCGTTCCAGTGCTGCCGGGGCGCTGGCGAGGTAGTTTTCGCTGTACCAGCCGTTGCGCAGCAGGGTGTAAACGAt                  <  2:6411396/93‑1 (MQ=255)

                                                                       cgcACCGATAAATACGCCGCGTTCCAGTGCTGCCGGGGCGCTGGCGAGGTAGTTTTCGCTGTACCAGCCGTTGCGCAGCAGGGTGTAAACGAt                  <  2:486968/93‑1 (MQ=255)

                                                                       cgcACCGATAAATACGCCGCGTTCCAGTGCTGCCGGGGCGCTGGCGAGGTAGTTTTCGCTGTACCAGCCGTTGCGCAGCAGGGTGTAAACGAt                  <  2:5294230/93‑1 (MQ=255)

                                                                        gcACCGATAAATACGCCGCGTTCCAGTGCTGCCGGGGCGCTGGCGAGGTAGTTTTCGCTGTACCAGCCGTTGCGCAGCAGGGTGTAAACGATg                 >  1:2927751/1‑93 (MQ=255)

                                                                         cACCGATAAATACGCCGCGTTCCAGTGCTGCCGGGGCGCTGGCGAGGTAGTTTTCGCTGTACCAGCCGTTGCGCAGCAGGGTGTAAACGATGc                >  2:4598788/1‑93 (MQ=255)

                                                                          cccGATAAATACGCCGCGTTCCAGTGCTGCCGGGGCGCTGGCGAGGTAGTTTTCGCTGTACCAGCCGTTGCGCAGCAGGGTGTAAACGATGcc               <  2:3715463/92‑1 (MQ=255)

                                                                          aCCGATAAATACGCCGCGTTCCAGTGCTGCCGGGGCGCTGGCGAGGTAGTTTTCGCTGTACCAGCCGTTGCGCAGCAGGGTGTAAACGATGcc               >  2:2879387/1‑93 (MQ=255)

                                                                          aCCGATAAATACGCCGCGTTCCAGTGCTGCCGGGGCGCTGGCGAGGTAGTTTTCGCTGTACCAGCCGTTGCGCAGCAGGGTGTAAACGATGcc               <  2:497796/93‑1 (MQ=255)

                                                                          aCCGATAAATACGCCGCGTTCCAGTGCTGCCGGGGCGCTGGCGAGGTAGTTTTCGCTGTACCAGCCGTTGCGCAGCAGGGTGTAAACGATGcc               <  1:6692104/93‑1 (MQ=255)

                                                                           ccGATAAATACGCCGCGTTCCAGTGCTGCCGGGGCGCTGGCGAGGTAGTTTTCGCTGTACCAGCCGTTGCGCAGCAGGGTGTAAACGATGCCa              <  1:690465/93‑1 (MQ=255)

                                                                           ccGATAAATACGCCGCGTTCCAGTGCTGCCGGGGCGCTGGCGAGGTAGTTTTCGCTGTACCAGCCGTTGCGCAGCAGGGTGTAAACGATGCCa              >  2:2020132/1‑93 (MQ=255)

                                                                             gATAAATACGCCGCGTTCCAGTGCTGCCGGGGCGCTGGCGAGGTAGTTTTCGCTGTACCAGCCGTTGCGCAGCAGGGTGTAAACGATGCCAGa            >  2:6452598/1‑93 (MQ=255)

                                                                             gATAAATACGCCGCGTTCCAGTGCTGCCGGGGCGCTGGCGAGGTAGTTTTCGCTGTACCAGCCGTTGCGCAGCAGGGTGTAAACGATGCCAGa            >  1:3637562/1‑93 (MQ=255)

                                                                              aTAAATACGCCGCGTTCCAGTGCTGCCGGGGCGCTGGCGAGGTAGTTTTCGCTGTACCAGCCGTTGCGCAGCAGGGTGTAAACGATGCCAGaa           <  2:2629421/93‑1 (MQ=255)

                                                                              aTAAATACGCCGCGTTCCAGTGCTGCCGGGGCGCTGGCGAGGTAGTTTTCGCTGTACCAGCCGTTGCGCAGCAGGGTGTAAACGATGCCAGaa           <  1:5826997/93‑1 (MQ=255)

                                                                               tAAATACGCCGCGTTCCAGTGCTGCCGGGGCGCTGGCGAGGTAGTTTTCGCTGTACCAGCCGTTGCGCAGCAGGGTGTAAACGATGCCAGAAt          <  2:110294/93‑1 (MQ=255)

                                                                                aaaTACGCCGCGTTCCAGTGCTGCCGGGGCGCTGGCGAGGTAGTTTTCGCTGTACCAGCCGTTGCGCAGCAGGGTGTAAACGATGCCAGAATc         <  1:2034936/93‑1 (MQ=255)

                                                                                aaaTACGCCGCGTTCCAGTGCTGCCGGGGCGCTGGCGAGGTAGTTTTCGCTGTACCAGCCGTTGCGCAGCAGGGTGTAAACGATGCCAGAATc         <  2:5129078/93‑1 (MQ=255)

                                                                                aaaTACGCCGCGTTCCAGTGCTGCCGGGGCGCTGGCGAGGTAGTTTTCGCTGTACCAGCCGTTGCGCAGCAGGGTGTAAACGATGCCAGAATc         <  1:4576247/93‑1 (MQ=255)

                                                                                 acctcgccgcGTTCCAGTGCTGCCGGGGCGCTGGCGAGGTAGTTTTCGCTGTACCAGCCGTTGCGCAGCAGGGTGTAAAc                     <  2:2299858/76‑1 (MQ=255)

                                                                                  aTACGCCGCGTTCCAGTGCTGCCGGGGCGCTGGCGAGGTAGTTTTCGCTGTACCAGCCGTTGCGCAGCAGGGTGTAAACGATGCCAGAATcag       >  2:6100354/1‑93 (MQ=255)

                                                                                   tACGCCGCGTTCCAGTGCTGCCGGGGCGCTGGCGAGGTAGTTTTCGCTGTACCAGCCGTTGCGCAGCAGGGTGTAAACGATGCCAGAATcagc      >  1:5285076/1‑93 (MQ=255)

                                                                                      gCCGCGTTCCAGTGCTGCCGGGGCGCTGGCGAGGTAGTTTTCGCTGTACCAGCCGTTGCGCAGCAGGGTGTAAACGATGCCAGAATcagccag   >  1:5396105/1‑93 (MQ=255)

                                                                                       ccGCGTTCCAGTGCTGCCGGGGCGCTGGCGAGGTAGTTTTCGCTGTACCAGCCGTTGCGCAGCAGGGTGTAAACGATGCCAGAATcagccagc  >  2:593033/1‑93 (MQ=255)

TCGCTAATCACGCGTGCCGCAGCTGCCGCATAATCTGCCCGCGTTGCTGAGGCGATTTTGCCATCGCCCGCCGCACCGATAAATACGCCGTGTTCCAGTGCTGCCGGGGCGCTGGCGAGGTAGTTTTCGCTGTACCAGCCGTTGCGCAGCAGGGTGTAAACGATGCCAGAATCAGCCAGC  >  NC_000913/4431052‑4431231

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 18 ≤` `ATCG/ATCG` `< 26 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`