BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,462,876	T→C	V293V (GTA→GTG)	treB ←	PTS trehalose‑specific system enzyme IIBC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,462,876	0	T	C	100.0%	801.6 / NA	249	V293V (GTA→GTG)	treB	PTS trehalose‑specific system enzyme IIBC
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (126/123); total (126/123)

Only 100 of 251 total aligned reads displayed.

CTGGTGTACACCGGTGATCACCAGCGGGGCGTACAGGAAGCCAAACAATGCTGCGCCAATCGGAGCAAAGCTGCCGGTCATCAGGTGACGTACCGCAAAGGCAACGCCATCGCCAATCATGCGACCAAACGGACCAATCAGCGCATGGGCGAGGAACACCGCGAGGATCAGCGAACAGAC  >  NC_000913/4462786‑4462965

cTGGTGTACACCGGTGATCACCAGCGGGGCGTACAGGAAGCCAAACAATGCTGCGCCAATCGGAGCAAAGCTGCCGGTCATCAGGTGACGCAc                                                                                         >  1:2531246/1‑93 (MQ=255)

 tGGTGTACACCGGTGATCACCAGCGGGGCGTACAGGAAGCCAAACAATGCTGCGCCAATCGGAGCAAAGCTGCCGGTCATCAGGTGACGCAcc                                                                                        <  1:678217/93‑1 (MQ=255)

 tGGTGTACACCGGTGATCACCAGCGGGGCGTACAGGAAGCCAAACAATGCTGCGCCAATCGGAGCAAAGCTGCCGGTCATCAGGTGACGCAcc                                                                                        >  2:3638872/1‑93 (MQ=255)

 tGGTGTACACCGGTGATCACCAGCGGGGCGTACAGGAAGCCAAACAATGCTGCGCCAATCGGAGCAAAGCTGCCGGTCATCAGGTGACGCAcc                                                                                        >  1:3028767/1‑93 (MQ=255)

         acCGGTGATCACCAGCGGGGCGTACAGGAAGCCAAACAATGCTGCGCCAATCGGAGCAAAGCTGCCGGTCATCAGGTGACGCACCGCAAAGGc                                                                                <  2:5432609/93‑1 (MQ=255)

          ccGGTGATCACCAGCGGGGCGTACAGGAAGCCAAACAATGCTGCGCCAATCGGAGCAAAGCTGCCGGTCATCAGGTGACGCACCGCAAAGGCa                                                                               <  1:783399/93‑1 (MQ=255)

            ggTGATCACCAGCGGGGCGTACAGGAAGCCAAACAATGCTGCGCCAATCGGAGCAAAGCTGCCGGTCATCAGGTGACGCACCGCAAAGGCAAc                                                                             >  2:3774027/1‑93 (MQ=255)

            ggTGATCACCAGCGGGGCGTACAGGAAGCCAAACAATGCTGCGCCAATCGGAGCAAAGCTGCCGGTCATCAGGTGACGCACCGCAAAGGCAAc                                                                             >  2:4088596/1‑93 (MQ=255)

              tGATCACCAGCGGGGCGTACAGGAAGCCAAACAATGCTGCGCCAATCGGAGCAAAGCTGCCGGTCATCAGGTGACGCACCGCAAAGGCAACGc                                                                           <  1:2729190/93‑1 (MQ=255)

              ggATCACCAGCGGGGCGTACAGGAAGCCAAACAATGCTGCGCCAATCGGAGCAAAGCGGCCGGTCATCAGGTGACGCACCGCAAAGGCAACGc                                                                           <  2:6410522/92‑1 (MQ=255)

               gATCACCAGCGGGGCGTACAGGAAGCCAAACAATGCTGCGCCAATCGGAGCAAAGCTGCCGGTCATCAGGTGACGCACCGCAAAGGCAACGcc                                                                          <  2:1433979/93‑1 (MQ=255)

                aTCACCAGCGGGGCGTACAGGAAGCCAAACAATGCTGCGCCAATCGGAGCAAAGCTGCCGGTCATCAGGTGACGCACCGCAAAGGCAACGCCa                                                                         >  1:2675130/1‑93 (MQ=255)

                 tCACCAGCGGGGCGTACAGGAAGCCAAACAATGCTGCGCCAATCGGAGCAAAGCTGCCGGTCATCAGGTGACGCACCGCAAAGGCAACGCCAt                                                                        >  2:2347555/1‑93 (MQ=255)

                 tCACCAGCGGGGCGTACAGGAAGCCAAACAATGCTGCGCCAATCGGAGCAAAGCTGCCGGTCATCAGGTGACGCACCGCAAAGGCAACGCCAt                                                                        <  1:1744626/93‑1 (MQ=255)

                  cACCAGCGGGGCGTACAGGAAGCCAAACAATGCTGCGCCAATCGGAGCAAAGCTGCCGGTCATCAGGTGACGCACCGCAAAGGCAACGCCATc                                                                       <  1:2572438/93‑1 (MQ=255)

                  cACCAGCGGGGCGTACAGGAAGCCAAACAATGCTGCGCCAATCGGAGCAAAGCTGCCGGTCATCAGGTGACGCACCGCAAAGGCAACGCCATc                                                                       >  1:4055535/1‑93 (MQ=255)

                   aCCAGCGGGGCGTACAGGAAGCCAAACAATGCTGCGCCAATCGGAGCAAAGCTGCCGGTCATCAGGTGACGCACCGCAAAGGCAACGCCATCg                                                                      >  2:127253/1‑93 (MQ=255)

                   aCCAGCGGGGCGTACAGGAAGCCAAACAATGCTGCGCCAATCGGAGCAAAGCTGCCGGTCATCAGGTGACGCACCGCAAAGGCAACGCCATCg                                                                      >  2:6516228/1‑93 (MQ=255)

                    ccAGCGGGGCGTACAGGAAGCCAAACAATGCTGCGCCAATCGGAGCAAAGCTGCCGGTCATCAGGTGACGCACCGCAAAGGCAACGCCATCGc                                                                     >  2:2511990/1‑93 (MQ=255)

                    ccAGCGGGGCGTACAGGAAGCCAAACAATGCTGCGCCAATCGGAGCAAAGCTGCCGGTCATCAGGTGACGCACCGCAAAGGCAACGCCATCGc                                                                     <  2:3230499/93‑1 (MQ=255)

                      aGCGGGGCGTACAGGAAGCCAAACAATGCTGCGCCAATCGGAGCAAAGCTGCCGGTCATCAGGTGACGCACCGCAAAGGCAACGCCATCGCCa                                                                   >  1:5563930/1‑93 (MQ=255)

                        cGGGGCGTACAGGAAGCCAAACAATGCTGCGCTAATCGGAGCAAAGCTGCCGGTCATCAGGTGACGCACCGCAAAGGCAACGCCATCGCCAAt                                                                 <  1:2909840/93‑1 (MQ=255)

                         ggggCGTACAGGAAGCCAAACAATGCTGCGCCAATCGGAGCAAAGCTGCCGGTCATCAGGTGACGCACCGCAAAGGCAACGCCATCGCCAATc                                                                >  2:1212550/1‑93 (MQ=255)

                          gggCGTACAGGAAGCCAAACAATGCTGCGCCAATCGGAGCAAAGCTGCCGGTCATCAGGTGACGCACCGCAAAGGCAACGCCATCGCCAATCa                                                               <  1:1001673/93‑1 (MQ=255)

                           ggCGTACAGGAAGCCAAACAATGCTGCGCCAATCGGAGCAAAGCTGCCGGTCATCAGGTGACGCACCGCAAAGGCAACGCCATCGCCAATCAt                                                              <  2:524932/93‑1 (MQ=255)

                           ggCGTACAGGAAGCCAAACAATGCTGCGCCAATCGGAGCAAAGCTGCCGGTCATCAGGTGACGCACCGCAAAGGCAACGCCATCGCCAATCAt                                                              <  1:722754/93‑1 (MQ=255)

                             cGTACAGGAAGCCAAACAATGCTGCGCCAATCGGAGCAAAGCTGCCGGTCATCAGGTGACGCACCGCAAAGGCAACGCCATCGCCAATCATGc                                                            <  2:1591779/93‑1 (MQ=255)

                             cGTACAGGAAGCCAAACAATGCTGCGCCAATCGGAGCAAAGCTGCCGGTCATCAGGTGACGCACCGCAAAGGCAACGCCATCGCCAATCATGc                                                            <  1:1318574/93‑1 (MQ=255)

                              gTACAGGAAGCCAAACAATGCTGCGCCAATCGGAGCAAAGCTGCCGGTCATCAGGTGACGCACCGCAAAGGCAACGCCATCGCCAATCATGCg                                                           >  2:2416828/1‑93 (MQ=255)

                              gTACAGGAAGCCAAACAATGCTGCGCCAATCGGAGCAAAGCTGCCGGTCATCAGGTGACGCACCGCAAAGGCAACGCCATCGCCAATCATGCg                                                           >  1:1241167/1‑93 (MQ=255)

                               tACAGTAAGCCAAACAATGCTGCGCCAATCGGAGCAAAGCTGCCGGTCATCAGGTGACGCACCGCAAAGGCAACGCCATCGCCAATCATGCGa                                                          <  1:2019475/93‑1 (MQ=255)

                                aCAGGAAGCCAAACAATGCTGCGCCAATCGGAGCAAAGCTGCCGGTCATCAGGTGACGCACCGCAAAGGCAACGCCAACGCCAATCATGCGAc                                                         >  1:6356220/1‑93 (MQ=255)

                                 cAGGAAGCCAAACAATGCTGCGCCAATCGGAGCAAAGCTGCCGGTCATCAGGTGACGCACCGCAAAGGCAACGCCATCGCCAATCATGCGAcc                                                        <  1:827655/93‑1 (MQ=255)

                                 cAGGAAGCCAAACAATGCTGCGCCAATCGGAGCAAAGCTGCCGGTCATCAGGTGACGCACCGCAAAGGCAACGCCATCGCCAATCATGCGAcc                                                        <  1:5682891/93‑1 (MQ=255)

                                   ggAAGCCAAACAATGCTGCGCCAATCGGAGCAAAGCTGCCGGTCATCAGGTGACGCACCGCAAAGGCAACGCCATCGCCAATCATGCGACCaa                                                      >  1:2678007/1‑93 (MQ=255)

                                   ggAAGCCAAACAATGCTGCGCCAATCGGAGCAAAGCTGCCGGTCATCAGGTGACGCACCGCAAAGGCAACGCCATCGCCAATCATGCGACCaa                                                      >  1:4585746/1‑93 (MQ=255)

                                   ggAAGCCAAACAATGCTGCGCCAATCGGAGCAAAGCTGCCGGTCATCAGGTGACGCACCGCAAAGGCAACGCCATCGCCAATCATGCGACCaa                                                      >  2:6125345/1‑93 (MQ=255)

                                    gAAGCCAAACAATGCTGCGCCAATCGGAGCAAAGCTGCCGGTCATCAGGTGACGCACCGCAAAGGCAACGCCATCGCCAATCATGCGACCaaa                                                     <  1:160236/93‑1 (MQ=255)

                                     aaGCCAAACAATGCTGCGCCAATCGGAGCAAAGCTGCCGGTCATCAGGTGACGCACCGCAAAGGCAACGCCATCGCCAATCATGCGACCAAAc                                                    >  1:3515684/1‑93 (MQ=255)

                                      aGCCAAACAATGCTGCGCCAATCGGAGCAAAGCTGCCGGTCATCAGGTGACGCACCGCAAAGGCAACGCCATCGCCAATCATGCGACCAAACg                                                   >  2:6185563/1‑93 (MQ=255)

                                      aGCCAAACAATGCTGCGCCAATCGGAGCAAAGCTGCCGGTCATCAGGTGACGCACCGCAAAGGCAACGCCATCGCCAATCATGCGACCAAACg                                                   <  2:3720418/93‑1 (MQ=255)

                                      aGCCAAACAATGCTGCGCCAATCGGAGCAAAGCTGCCGGTCATCAGGTGACGCACCGCAAAGGCAACGCCATCGCCAATCATGCGACCAAACg                                                   >  2:4993860/1‑93 (MQ=255)

                                        ccAAACAATGCTGCGCCAATCGGAGCAAAGCTGCCGGTCATCAGGTGACGCACCGCAAAGGCAACGCCATCGCCAATCATGCGACCAAACGGa                                                 <  2:2226379/93‑1 (MQ=255)

                                        ccAAACAATGCTGCGCCAATCGGAGCAAAGCTGCCGGTCATCAGGTGACGCACCGCAAAGGCAACGCCATCGCCAATCATGCGACCAAACGGa                                                 >  1:400469/1‑93 (MQ=255)

                                         cAAACAATGCTGCGCCAATCGGAGCAAAGCTGCCGGTCATCAGGTGACGCACCGCAAAGGCAACGCCATCGCCAATCATGCGACCAAACGGAc                                                >  1:3945211/1‑93 (MQ=255)

                                           aaCAATGCTGCGCCAATCGGAGCAAAGCTGCCGGTCATCAGGTGACGCACCGCAAAGGCAACGCCATCGCCAATCATGCGACCAAACGGACCa                                              >  2:2673134/1‑93 (MQ=255)

                                                 gctgcGCCAATCGGAGCAAAGCTGCCGGTCATCAGGTGACGCACCGCAAAGGCAACGCCATCGCCAATCATGCGACCAAACGGACCAATCAgc                                        >  1:3162151/1‑93 (MQ=255)

                                                   tgcGCCAATCGGAGCAAAGCTGCCGGTCATCAGGTGACGCACCGCAAAGGCAACGCCATCGCCAATCATGCGACCAAACGGACCAATCAgcgc                                      <  1:3616995/93‑1 (MQ=255)

                                                    gcgcCAATCGGAGCAAAGCTGCCGGTCATCAGGTGACGCACCGCAAAGGCAACGCCATCGCCAATCATGCGACCAAACGGACCAATCAGCGCa                                     <  1:5095722/93‑1 (MQ=255)

                                                    gcgcCAATCGGAGCAAAGCTGCCGGTCATCAGGTGACGCACCGCAAAGGCAACGCCATCGCCAATCATGCGACCAAACGGACCAATCAGCGCa                                     >  2:676343/1‑93 (MQ=255)

                                                    gcgcCAATCGGAGCAAAGCCGCCGGTCATCAGGTGACGCACCGCAAAGGCAACGCCATCGCCAATCATGCGACCAAACGGACCAATCAGCGCa                                     >  1:6560931/1‑93 (MQ=255)

                                                      gcCAATCGGAGCAAAGCTGCCGGTCATCAGGTGAGGCACCGCAAAGGCAACGCCATCGCCAATCATGCGACCAAACGGACCAATCAGCGCATg                                   <  1:1387303/93‑1 (MQ=255)

                                                      gcCAATCGGAGCAAAGCTGCCGGTCATCAGGTGACGCACCGCAAAGGCAACGCCATCGCCAATCATGCGACCAAACGGACCAATCAGCGCATg                                   <  2:1653821/93‑1 (MQ=255)

                                                        cAATCGGAGCAAAGCTGCCGGTCATCAGGTGACGCACCGCAAAGGCAACGCCATCGCCAATCATGCGACCAAACGGACCAATCAGCGCATggg                                 >  1:1505414/1‑93 (MQ=255)

                                                        cAATCGGAGCAAAGCTGCCGGTCATCAGGTGACGCACCGCAAAGGCAACGCCATCGCCAATCATGCGACCAAACGGACCAATCAGCGCATggg                                 >  1:436104/1‑93 (MQ=255)

                                                          aTCGGAGCAAAGCTGCCGGTCATCAGGTGACGCACCGCAAAGGCAACGCCATCGCCAATCATGCGACGAAACGGACCAATCAGCGCATGGGCg                               >  2:5193688/1‑93 (MQ=255)

                                                           tCGGAGCAAAGCTGCCGGTCATCAGGTGACGCACCGCAAAGGCAACGCCATCGCCAATCATGCGACCAAACGGACCAATCAGCGCATGGGCGa                              <  2:4528363/93‑1 (MQ=255)

                                                             ggAGCAAAGCTGCCGGTCATCAGGTGACGCACCGCAAAGGCAACGCCATCGCCAATCATGCGACCAAACGGACCAATCAGCGCATGGGCGAgg                            >  2:2978821/1‑93 (MQ=255)

                                                             ggAGCAAAGCTGCCGGTCATCAGGTGACGCACCGCAAAGGCAACGCCATCGCCAATCATGCGACCAAACGGACCAATCAGCGCATGGGCGAgg                            <  1:3121920/93‑1 (MQ=255)

                                                              gAGCAAAGCTGCCGGTCATCAGGTGACGCACCGCAAAGGCAACGCCATCGCCAATCATGCGACCAAACGGACCAATCAGCGCATGGGCGAGGa                           <  1:2271519/93‑1 (MQ=255)

                                                              gAGCAAAGCTGCCGGTCATCAGGTGACGCACCGCAAAGGCAACGCCATCGCCAATCATGCGACCAAACGGACCAATCAGCGCATGGGCGAGGa                           >  1:6104226/1‑93 (MQ=255)

                                                              gAGCAAAGCTGCCGGTCATCAGGTGACGCACCGCAAAGGCAACGCCATCGCCAATCATGCGACCAAACGGACCAATCAGCGCATGGGCGAGGa                           >  1:4621731/1‑93 (MQ=255)

                                                               aGCAAAGCTGCCGGTCATCAGGTGACGCACCGCAAAGGCAACGCCATCGCCAATCATGCGACCAAACGGACCAATCAGCGCATGGGCGAGGaa                          <  2:2038345/93‑1 (MQ=255)

                                                                gCAAAGCTGCCGGTCATCAGGTGACGCACCGCAAAGGCAACGCCATCGCCAATCATGCGACCAAACGGACCAATCAGCGCATGGGCGAGGAac                         <  2:6346948/93‑1 (MQ=255)

                                                                 cAAAGCTGCCGGTCATCAGGTGACGCACCGCAAAGGCAACGCCATCGCCAATCATGCGACCAAACGGACCAATCAGCGCATGGGCGAGGAaca                        <  2:5957239/93‑1 (MQ=255)

                                                                  aaaGCTGCCGGTCATCAGGTGACGCACCGCAAAGGCAACGCCATCGCCAATCATGCGACCAAACGGACCAATCAGCGCATGGGCGAGGAacac                       <  1:607730/93‑1 (MQ=255)

                                                                  aaaGCTGCAGCTCATCAGGTGACGCACCGCAAAGGCAACGCCATCGCCAATCATGCGACCAAACGGACCAATCAGCGCATGGGCGAGGAacac                       <  2:6669703/93‑1 (MQ=255)

                                                                   aaGCTGCCGGTCATCAGGTGACGCACCGCAAAGGCAACGCCATCGCCAATCATGCGACCAAACGGACCAATCAGCGCATGGGCGAGGAACAcc                      <  1:3394379/93‑1 (MQ=255)

                                                                   aaGCTGCCGGTCATCAGGTGACGCACCGCAAAGGCAACGCCATCGCCAATCATGCGACCAAACGGACCAATCAGCGCATGGGCGAGGAACAcc                      >  1:395662/1‑93 (MQ=255)

                                                                   aaGCTGCCGGTCATCAGGTGACGCACCGCAAAGGCAACGCCATCGCCAATCATGCGACCAAACGGACCAATCAGCGCATGAGCGAGGAACAcc                      >  2:3515403/1‑93 (MQ=255)

                                                                    aGCTGCCGGTCATCAGGTGACGCACCGCAAAGGCAACGCCATCGCCAATCATGCGACCAAACGGACCAATCAGCGCATGGGCGAGGCACACcg                     >  1:547642/1‑93 (MQ=255)

                                                                    aGCTGCCGGTCATCAGGTGACGCACCGCAAAGGCAACGCCATCGCCAATCATGCGACCAAACGGACCAATCAGCGCATGGGCGAGGAACACcg                     <  2:4427738/93‑1 (MQ=255)

                                                                    aGCTGCCGGTCATCAGGTGACGCACCGCAAAGGCAACGCCATCGCCAATCATGCGACCAAACGGACCAATCAGCGCATGGGCGAGGAACACcg                     >  2:906379/1‑93 (MQ=255)

                                                                       tGCCGGTCATGAGGTGACGCACCGCAAAGGCAACGCCATCGCCAATCATGCGACCAAACGGACCAATCAGCGCATGGGCGAGGAACACCGCGa                  <  1:5789736/93‑1 (MQ=255)

                                                                       tGCCGGTCATCAGGTGACGCACCGCAAAGGCAACGCCATCGCCAATCATGCGACCAAACGGACCAATCAGCGCATGGGCGAGGAACACCGCGa                  <  1:2355681/93‑1 (MQ=255)

                                                                        tccGGTCATCAGGTGACGCACCGAAAAGGCAACGCCATCGCCAATCATGCGACCAAACGGACCAATCAGCGCATGGGCGAGGAACACCGCGAg                 <  1:368107/92‑1 (MQ=255)

                                                                        gCCGGTCATCAGGTGACGCACCGCAAAGGCAACGCCATCGCCAATCATGCGACCAAACGGACCAATCAGCGCATGGGCGAGGAACACCGCGAg                 <  2:6232470/93‑1 (MQ=255)

                                                                          cGGTCATCAGGTGACGCACCGCAAAGGCAACGCCATCGCCAATCATGCGACCAAACGGACCAATCAGCGCATGGGCGAGGAACACCGCGAGGa               >  2:276054/1‑93 (MQ=255)

                                                                          cGGTCATCAGGTGACGCACCGCAAAGGCAACGCCATCGCCAATCATGCGACCAAACGGACCAATCAGCGCATGGGCGAGGAACACCGCGAGGa               <  2:1156166/93‑1 (MQ=255)

                                                                           ggTCATCAGGTGACGCACCGCAAAGGCAACGCCATCGCCAATCATGCGACCAAACGGACCAATCAGCGCATGGGCGAGGAACACCGCGAGGAt              <  2:3794598/93‑1 (MQ=255)

                                                                            gTCATCAGGTGACGCACCGCAAAGGCAACGCCATCGCCAATCATGCGACCAAACGGACCAATCAGCGCATGGGCGAGGAACACCGCGAGGATc             <  1:5212644/93‑1 (MQ=255)

                                                                            gTCATCAGGTGACGCACCGCAAAGGCAACGCCATCGCCAATCATGCGACCAAACGGACCAATCAGCGCATGGGCGAGGAACACCGCGAGGATc             <  2:6022373/93‑1 (MQ=255)

                                                                            gTCATCAGGTGACGCACCGCAAAGGCAACGCCATCGCCAATCATGCGACCAAACGGACCAATCAGCGCATGGGCGAGGAACACCGCGAGGATc             >  2:5777946/1‑93 (MQ=255)

                                                                             tcatcaGGTGACGCACCGCAAAGGCAACGCCATCGCCAATCATGCGACCAAACGGACCAATCAGCGCATGGGCGAGGAACACCGCGAGGATCa            <  2:1546759/93‑1 (MQ=255)

                                                                             tcatcaGGTGACGCACCGCAAAGGCAACGCCATCGCCAATCATGCGACCAAACGGACCAATCAGCGCATGGGCGAGGAACACCGCGAGGATCa            <  2:5686382/93‑1 (MQ=255)

                                                                              catcaGGTGACGCACCGCAAAGGCAACGCCATCGCCAATCATGCGACCAAACGGACCAATCAGCGCATGGGCGAGGAACACCGCGAGGATCAg           >  1:2118155/1‑93 (MQ=255)

                                                                              catcaGGTGACGCACCGCAAAGGCAACGCCATCGCCAATCATGCGACCAAACGGACCAATCAGCGCATGGGCGAGGAACACCGCGAGGATCAg           >  2:6463589/1‑93 (MQ=255)

                                                                              catcaGGTGACGCACCGCAAAGGCAACGCCATCGCCAATCATGCGACCAAACGGACCAATCAGCGCATGGGCGAGGAACACCGCGAGGATCAg           <  2:2247285/93‑1 (MQ=255)

                                                                               atcaGGTGACGCACCGCAAAGGCAACGCCATCGCCAATCATGCGACCAAACGGACCAATCAGCGCATGGGCGAGGAACACCGCGAGGATCAGc          <  2:3409459/93‑1 (MQ=255)

                                                                                 caGGTGACGCACCGCAAAGGCAACGCCATCGCCAATCATGCGACCAAACGGACCAATCAGCGCATGGGCGAGGAACACCGCGAGGATCAGCGa        >  2:5873285/1‑93 (MQ=255)

                                                                                  aGGTGACGCACCGCAAAGGCAACGCCATCGCCAATCATGCGACCAAACGGACCAATCAGCGCATGGGCGAGGAACACCGCGAGGATCAGCGaa       >  2:5940503/1‑93 (MQ=255)

                                                                                  aGGTGACGCACCGCAAAGGCAACGCCATCGCCAATCATGCGACCAAACGGACCAATCAGCGCATGGGCGAGGAACACCGCGAGGATCAGCGaa       >  2:41935/1‑93 (MQ=255)

                                                                                   ggTGACGCACCGCAAAGGCAACGCCATCGCCAATCATGCGACCAAACGGACCAATCAGCGCATGGGCGAGGAACACCGCGAGGATCAGCGAAc      <  2:4760472/93‑1 (MQ=255)

                                                                                   ggTGACGCACCGCAAAGGCAACGCCATCGCCAATCATGCGACCAAACGGACCAATCAGCGCATGGGCGAGGAACACCGCGAGGATCAGCGAAc      >  2:313333/1‑93 (MQ=255)

                                                                                    gTGACGCACCGCAAAGGCAACGCCATCGCCAATCATGCGACCAAACGGACCAATCAGCGCATGGGCGAGGAACACCGCGAGGATCAGCGAACa     <  1:3330188/93‑1 (MQ=255)

                                                                                      gACGCACCGCAAAGGCAACGCCATCGCCAATCATGCGACCAAACGGACCAATCAGCGCATGGGCGAGGAACACCGCGAGGATCAGCGAACAGa   <  2:1970410/93‑1 (MQ=255)

                                                                                      gACGCACCGCAAAGGCAACGCCATCGCCAATCATGCGACCAAACGGACCAATCAGCGCATGGGCGAGGAACACCGCGAGGATCAGCGAACAGa   <  2:2506212/93‑1 (MQ=255)

                                                                                      gACGCACCGCAAAGGCAACGCCATCGCCAATCATGCGACCAAACGGACCAATCAGCGCATGGGCGAGGAACACCGCGAGGATCAGCGAACAGa   <  1:4531516/93‑1 (MQ=255)

                                                                                       aCGCACCGCAAAGGCAACGCCATCGCCAATCATGCGACCAAACGGACCAATCAGCGCATGGGCGAGGAACACCGCGAGGATCAGCGAACAGAc  >  1:1070096/1‑93 (MQ=255)

                                                                                       aCGCACCGCAAAGGCAACGCCATCGCCAATCATGCGACCAAACGGACCAATCAGCGCATGGGCGAGGAACACCGCGAGGATCAGCGAACAGAc  <  2:3947857/93‑1 (MQ=255)

CTGGTGTACACCGGTGATCACCAGCGGGGCGTACAGGAAGCCAAACAATGCTGCGCCAATCGGAGCAAAGCTGCCGGTCATCAGGTGACGTACCGCAAAGGCAACGCCATCGCCAATCATGCGACCAAACGGACCAATCAGCGCATGGGCGAGGAACACCGCGAGGATCAGCGAACAGAC  >  NC_000913/4462786‑4462965

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 20 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`