Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,483,536 T→C intergenic (‑17/‑250) pepA ← / → yjgP aminopeptidase A/I/hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,483,5360TC99.0% 1032.7 / ‑3.1 314intergenic (‑17/‑250)pepA/yjgPaminopeptidase A/I/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (1/2);  new base C (146/165);  total (147/167)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 316 total aligned reads displayed.

GGTTCGAAGACGCCCACGACGATGCAGGCACTCCGCTGTTTCTCCGGGCTACCGCTTTTTACACTAAACTCCATGCACTACGCTCCTGAATCTTAAAGACAACGGCGGTGGCTACAGATAGAATTGCAAGCTTTCGTAACTCATGTCCGCTGTTGCGATGACTTCGTGTTAATCTTAACG  >  NC_000913/4483446‑4483625
                                                                                          |                                                                                         
ggTTCGAAGACGCCCACGACGATGCAGGCACTCCGCTGTTTCTCCGGGCTACCGCTTTTTACACTAAACTCCATGCACTACGCTCCTGAACCt                                                                                         <  2:1113865/93‑1 (MQ=255)
 gTTCGAAGACGCCCACGACGATGCAGGCACTCCGCTGTTTCTCCGGGCTACCGCTTTTTACACTAAACTCCATGCACTACGCTCCTGAACCtt                                                                                        <  1:579855/93‑1 (MQ=255)
   tCGAAGACGCCCACGACGATGCAGGCACTCCGCTGTTTCTCCGGGCTACCGCTTTTTACACTAAACTCCATGCACTACGCTCCTGAACCTTaa                                                                                      <  1:1771205/93‑1 (MQ=255)
    cGAAGACGCCCACGACGATGCAGGCACTCCGCTGTTTCTCCGGGCTACCGCTTTTTACACTAAACTCCATGCACTACGCTCCTGAACCTTaaa                                                                                     <  1:3337481/93‑1 (MQ=255)
    cGAAGACGCCCACGACGATGCAGGCACTCCGCTGTTTCTCCGGGCTACCGCTTTTTACACTAAACTCCATGCACTACGCTCCTGAACCTTaaa                                                                                     <  1:6232088/93‑1 (MQ=255)
     gAAGACGCCCACGACGATGCAGGCACTCCGCTGTTTCTCCGGGCTACCGCTTTTTACACTAAACTCCATGCACTACGCTCCTGAACCTTAAAg                                                                                    >  1:186035/1‑93 (MQ=255)
     gAAGACGCCCACGACGATGCAGGCACTCCGCTGTTTCTCCGGGCTACCGCTTTTTACACTAAACTCCATGCACTACGCTCCTGAACCTTAAAg                                                                                    <  2:4936511/93‑1 (MQ=255)
       aGACGCCCACGACGATGCAGGCACTCCGCTGTTTCTCCGGGCTACCGCTTTTTACACTAAACTCCATGCACTACGCTCCTGAACCTTAAAGAc                                                                                  >  1:4204075/1‑93 (MQ=255)
       aGACGCCCACGACGATGCAGGCACTCCGCTGTTTCTCCGGGCTACCGCTTTTTACACTAAACTCCATGCACTACGCTCCTGAACCTTAAAGAc                                                                                  >  2:5727628/1‑93 (MQ=255)
        gACGCCCACGACGATGCAGGCACTCCGCTGTTTCTCCTGGCTACCGCTTTTTACACTAAACTCCATGCACTACGCTCCTGAACCTTAAAGACa                                                                                 >  1:1403253/1‑93 (MQ=255)
          cGCCCACGACGATGCAGGCACTCCGCTGTTTCTCCGGGCTACCGCTTTTTACACTAAACTCCATGCACTACGCTCCTGAACCTTAAAGACAAc                                                                               <  1:1293953/93‑1 (MQ=255)
          cGCCCACGACGATGCAGGCACTCCGCTGTTTCTCCGGGCTACCGCTTTTTACACTAAACTCCATGCACTACGCTCCTGAACCTTAAAGACAAc                                                                               <  2:2798604/93‑1 (MQ=255)
          cGCCCACGACGATGCAGGCACTCCGCTGTTTCTCCGGGCTACCGCTTTTTACACTAAACTCCATGCACTACGCTCCTGAACCTTAAAGACAAc                                                                               <  1:5302355/93‑1 (MQ=255)
            cccACGACGATGCAGGCACTCCGCTGTTTCTCCGGGCTACCGCTTTTTACACTAAACTCCATGCACTACGCTCCTGAACCTTAAAGACAAcgg                                                                             <  1:3656618/93‑1 (MQ=255)
            cccACGACGATGCAGGCACTCCGCTGTTTCTCCGGGCTACCGCTTTTTACACTAAACTCCATGCACTACGCTCCTGAACCTTAAAGACAAcgg                                                                             >  1:2821239/1‑93 (MQ=255)
             ccACGACGATGCAGGCACTCCGCTGTTTCTCCGGGCTACCGCTTTTTACACTAAACTCCATGCACTACGCTCCTGAACCTTAAAGACAAcggc                                                                            >  2:6693694/1‑93 (MQ=255)
              cACGACGATGCAGGCACTCCGCTGTTTCTCCGGGCTACCGCTTTTTACACTAAACTCCATGCACTACGCTCCTGAACCTTAAAGACAAcggcg                                                                           >  2:3696460/1‑93 (MQ=255)
               acgacgATGCAGGCACTCCGCTGTTTCTCCGGGCTACCGCTTTTTACACTAAACTCCATGCACTACGCTCCTGAACCTTAAAGCCAAcggcgg                                                                          >  2:3540137/1‑93 (MQ=255)
               acgacgATGCAGGCACTCCGCTGTTTCTCCGGGCTACCGCTTTTTACACTAAACTCCATGCACTACGCTCCTGAACCTTAAAGACAAcggcgg                                                                          >  1:881129/1‑93 (MQ=255)
               acgacgATGCAGGCACTCCGCTGTTTCTCCGGGCTACCGCTTTTTACACTAAACTCCATGCACTACGCTCCTGAACCTTAAAGACAAcggcgg                                                                          >  2:5323239/1‑93 (MQ=255)
                cgacgaTGTAGGCACTCCGCTGTTTCTCCGGGCTACCGCTTTTTACACTAAACTCCATGCACTACGCTCCTGAACCTTAAAGACAACGGCGGt                                                                         <  1:2329139/93‑1 (MQ=255)
                cgacgaTGCAGGCACTCCGCTGTTTCTCCGGGCTACCGCTTTTTACACTGAACTCCATGCACTACGCTCCTGAACCTTAAAGACAACGGCGGt                                                                         <  2:2912377/93‑1 (MQ=255)
                cgacgaTGCAGGCACTCCGCTGTTTCTCCGGGCTACCGCTTTTTACACTAAACTCCATGCACTACGCTCCTGAACCTTAAAGACAACGGCGGt                                                                         <  1:1136960/93‑1 (MQ=255)
                 gacgaTGCAGGCACTCCGCTGTTTCTCCGGGCTACCGCTTTTTACACTAAACTCCATGCACTACGCTCCTGAACCTTAAAGACAACGGCGGTg                                                                        >  2:764788/1‑93 (MQ=255)
                 gacgaTGCAGGCACTCCGCTGTTTCTCCGGGCTACCGCTTTTTACACTAAACTCCATGCACTACGCTCCTGAACCTTAAAGACAACGGCGGTg                                                                        <  2:4445947/93‑1 (MQ=255)
                 gacgaTGCAGGCACTCCGCTGTTTCTCCGGGCTACCGCTTTTTACACTAAACTCCATGCACTACGCTCCTGAACCTTAAAGACAACGGCGGTg                                                                        <  2:6086363/93‑1 (MQ=255)
                  acgaTGCAGGCACTCCGCTGTTTCTCCGGGCTACCGCTTTTTACACTAAACTCCATGCACTACGCTCCTGAACCTTAAAGACAACGGCGGTgg                                                                       >  2:3208509/1‑93 (MQ=255)
                  acgaTGCAGGCACTCCGCTGTTTCTCCGGGCTACCGCTTTTTACACTAAACTCCATGCACTACGCTCCTGAACCTTAAAGACAACGGCGGTgg                                                                       >  2:5592777/1‑93 (MQ=255)
                  acgaTGCAGGCACTCCGCTGTTTCTCCGGGCTACCGCTTTTTACACTAAACTCCATGCACTACGCTCCTGAACCTTAAAGACAACGGCGGTgg                                                                       >  1:1476768/1‑93 (MQ=255)
                   cgaTGCAGGCACTCCGCTGTTTCTCCGGGCTACCGCTTTTTACACTAAACTCCATGCACTACGCTCCTGAACCTTAAAGACAACGGCGGTGGc                                                                      <  1:3400279/93‑1 (MQ=255)
                   cgaTGCAGGCACTCCGCTGTTTCTCCGGGCTACCGCTTTTTACACTAAACTCCATGCACTACGCTCCTGAACCTTAAAGACAACGGCGGTGGc                                                                      <  1:4730369/93‑1 (MQ=255)
                     aTGCAGGCACTCCGCTGTTTCTCCGGGCTACCGCTTTTTACACTAAACTCCATGCACTACGCTCCTGAACCTTAAAGACAACGGCGGTGGCTa                                                                    <  1:4031234/93‑1 (MQ=255)
                       gCAGGCACTCCGCTGTTTCTCCGGGCTACCGCTTTTTACACTAAACTCCATGCACTACGCTCCTGAACCTTAAAGACAACGGCGGTGGCTACa                                                                  <  2:1580401/93‑1 (MQ=255)
                       gCAGGCACTCCGCTGTTTCTCCGGGCTACCGCTTTTTACACTAAACTCCATGCACTACGCTCCTGAACCTTAAAGACAACGGCGGTGGCTACa                                                                  <  1:6296521/93‑1 (MQ=255)
                          ggCACTCCTCTGTTTCTCCGGGCTACCGCTTTTTACACTAAACTCCATGCACTACGCTCCTGAACCTTAAAGACAACGGCGGTGGCTACAGAt                                                               >  1:6517016/1‑93 (MQ=255)
                          ggCACTCCGCTGTTTCTCCGGGCTACCGCTTTTTACACTAAACTCCATGCACTACGCTCCTGAACCTTAAAGACAACGGCGGTGGCTACAGAt                                                               >  2:6256116/1‑93 (MQ=255)
                          ggCACTCCGCTGTTTCTCCGGGCTACCGCTTTTTACACTAAACTCCATGCACTACGCTCCTGAACCTTAAAGACAACGGCGGTGGCTACAGAt                                                               >  1:2928521/1‑93 (MQ=255)
                           gCACTCCGCTGTTTCTCCGGGCTACCGCTTTTTACACTAAACTCCATGCACTACGCTCCTGAACCTTAAAGACAACGGCGGTGGCTACAGATa                                                              <  1:1550981/93‑1 (MQ=255)
                           gCACTCCGCTGTTTCTCCGGGCTACCGCTTTTTACACTAAACTCCATGCACTACGCTCCTGAACCTTAAAGACAACGGCGGTGGCTACAGATa                                                              <  2:2256282/93‑1 (MQ=255)
                             ccTCCGCTGTTTCTCCGGGCTACCGCTTTTTACACTAAACTCCATGCACTACGCTCCTGAACCTTAAAGACAACGGCGGTGGCTACAGATAGa                                                            <  2:1951976/92‑1 (MQ=255)
                              cTCCGCTGTTTCTCCGGGCTACCGCTTTTTACACTAAACTCCATGCACTACGCTCCTGAACCTTAAAGACAACGGCGGTGGCTACAGATAGaa                                                           <  1:2587318/93‑1 (MQ=255)
                              cTCCGCTGTTGCTCCGGGCTACCGCTTTTTACACTAAACTCCATGCACTACGCTCCTGAACCTTAAAGACAACGGCGGTGGCTACAGATAGaa                                                           >  1:4073970/1‑93 (MQ=255)
                                  gCTGTTTCTCCGGGCTACCGCTTTTTACACTAAACTCCATGCACTACGCTCCTGAACCTTAAAGACAACGGCGGTGGCTACAGATAGAATTGc                                                       >  2:2882193/1‑93 (MQ=255)
                                  gCTGTTTCTCCGGGCTACCGCTTTTTACACTAAACTCCATGCACTACGCTCCTGAACCTTAAAGACAACGGCGGTGGCTACAGATAGAATTGc                                                       >  1:2005219/1‑93 (MQ=255)
                                    tGTTTCTCCGGGCTACCGCTTTTTACACTAAACTCCATGCACTACGCTCCTGAACCTTAAAGACAACGGCGGTGGCTACAGATAGAATTGCaa                                                     <  2:3629887/93‑1 (MQ=255)
                                    tGTTTCTCCGGGCTACCGCTTTTTACACTAAACTCCATGCACTACGCTCCTGAACCTTAAAGACAACGGCGGTGGCTACAGATAGAATTGCaa                                                     >  1:4659262/1‑93 (MQ=255)
                                    tGTTTCTCCGGGCTACCGCTTTTTACACTAAACTCCATGCACTACGCTCCTGAACCTTAAAGACAACGGCGGTGGCTACAGATAGAATTGCaa                                                     <  2:1279788/93‑1 (MQ=255)
                                       ttCTCCGGGCTACCGCTTTTTACACTAAACTCCATGCACTACGCTCCTGAACCTTAAAGACAACGGCGGTGGCTACAGATAGAATTGCAAGCt                                                  <  2:1841056/93‑1 (MQ=255)
                                        tctcCGGGCTACCGCTTTTTACACTAATCTCCATGCACTACGCTCCTGAACCTTAAAGACAACGGCGGTGGCTACAGATAGAATTGCAAGCtt                                                 <  1:1665080/93‑1 (MQ=255)
                                        tctcCGGGCTACCGCTTTTTACACTAAACTCCATGCACTACGCTCCTGAACCTTAAAGACAACGGCGGTGGCTACAGATAGAATTGCAAGCtt                                                 <  2:1706633/93‑1 (MQ=255)
                                         ctcCGGGCTACCGCTTTTTACACTAAACTCCATGCACTACGCTCCTGAACCTTAAAGACAACGGCGGTGGCTACAGATAGAATTGCAAGCttt                                                <  1:4804638/93‑1 (MQ=255)
                                           ccGGGCTACCGCTTTTTACACTAAACTCCATGCACTACGCTCCTGAACCTTAAAGACAACGGCGGTGGCTACAGATAGAATTGCAAGCTTTCg                                              <  1:3250185/93‑1 (MQ=255)
                                            cGGGCTACCGCTTTTTACACTAAACTCCATGCACTACGCTCCTGAACCTTAAAGACAACGGCGGTGGCTACAGATAGAATTGCAAGCTTTCGt                                             >  2:51306/1‑93 (MQ=255)
                                             gggCTACCGCTTTTTACACTAAACTCCATGCACTACGCTCCTGAACCTTAAAGACAACGGCGGTGGCTACAGATAGAATTGCAAGCTTTCGTa                                            <  1:5210127/93‑1 (MQ=255)
                                             gggCTACCGCTTTTTACACTAAACTCCATGCACTACGCTCCTGAACCTTAAAGACAACGGCGGTGGCTACAGATAGAATTGCAAGCTTTCGTa                                            >  2:2728420/1‑93 (MQ=255)
                                              ggCTACCGCTTTTTACACTAAACTCCATGCACTACGCTCCTGAACCTTAAAGACAACGGCGGTGGCTACAGATAGAATTGCAAGCTTTCGTaa                                           >  2:301190/1‑93 (MQ=255)
                                              ggCTACCGCTTTTTACACTAAACTCCATGCACTACGCTCCTGAACCTTAAAGACAACGGCGGTGGCTACAGATAGAATTGCAAGCTTTCGTaa                                           >  1:2379490/1‑93 (MQ=255)
                                              ggCTACCGCTTTTTACACTAAACTCCATGCACTACGCTCCTGAACCTTAAAGACAACGGCGGTGGCTACAGATAGAATTGCAAGCTTTCGTaa                                           >  2:2555535/1‑93 (MQ=255)
                                              ggCTACCGCTTTTTACACTAAACTCCATGCACTACGCTCCTGAACCTTAAAGACAACGGCGGTGGCTACAGATAGAATTGCAAGCTTTCGTaa                                           >  2:4602451/1‑93 (MQ=255)
                                               gCTACCGCTTTTTACACTAAACTCCATGCACTACGCTCCTGAACCTTAAAGACAACGGCGGTGGCTACAGATAGAATTGCAAGCTTTCGTAAc                                          <  1:1057820/93‑1 (MQ=255)
                                                cTACCGCTTTTTACACTAAACTCCATGCACTACGCTCCTGAACCTTAAAGACAACGGCGGTGGCTACAGATAGAATTGCAAGCTTTCGTAACt                                         <  1:2426658/93‑1 (MQ=255)
                                                  aCCGCTTTTTACACTAAACTCCATGCACTACGCTCCTGAACCTTAAAGACAACGGCGGTGGCTACAGATAGAATTGCAAGCTTTCGTAACTCa                                       <  2:6448859/93‑1 (MQ=255)
                                                  aCCGCTTTTTACACTAAACTCCATGCACTACGCTCCTGAACCTTAAAGACAACGGCGGTGGCTACAGATAGAATTGCAAGCTTTCGTAACTCa                                       <  1:192874/93‑1 (MQ=255)
                                                  aCCGCTTTTTACACTAAACTCCATGCACTACGCTCCTGAACCTTAAAGACAACGGCGGTGGCTACAGATAGAATTGCAAGCTTTCGTAACTCa                                       >  2:1359430/1‑93 (MQ=255)
                                                  aCCGCTTTTTACACTAAACTCCATGCACTACGCTCCTGAACCTTAAAGACAACGGCGGTGGCTACAGATAGAATTGCAAGCTTTCGTAACTCa                                       >  1:6712729/1‑93 (MQ=255)
                                                   ccGCTTTTTACACTAAACTCCATGCACTACGCTCCTGAACCTTAAAGACAACGGCGGTGGCTACAGATAGAATTGCAAGCTTTCGTAACTCAt                                      <  1:4286095/93‑1 (MQ=255)
                                                    cGCTTTTTACACTAAACTCCATGCACTACGCTCCTGAACCTTAAAGACAACGGCGGTGGCTACAGATAGAATTGCAAGCTTTCGTAACTCATg                                     <  2:2670723/93‑1 (MQ=255)
                                                     gCTTTTTACACTAAACTCCATGCACTACGCTCCTGAACCTTAAAGACAACGGCGGTGGCTACAGATAGAATTGCAAGCTTTCGTAACTCATGt                                    >  1:6359143/1‑93 (MQ=255)
                                                       tttttACACTAAACTCCATGCACTACGCTCCTGAACCTTAAAGACAACGGCGGTGGCTACAGATAGAATTGCAAGCTTTCGTAACTCATGTcc                                  >  1:4602919/1‑93 (MQ=255)
                                                         tttACACTAAACTCCATGCACTACGCTCCTGAACCTTAAAGACAACGGCGGTGGCTACAGATAGAATTGCAAGCTTTCGTAACTCATGTCCGc                                <  2:1619328/93‑1 (MQ=255)
                                                          ttACACTAAACTCCATGCACTACGCTCCTGAACCTTAAAGACAACGGCGGTGGCTACAGATAGAATTGCAAGCTTTCGTAACTCATGTCCGCt                               <  1:6109880/93‑1 (MQ=255)
                                                           tACACTAAGCTCCATGCACTACGCTCCTGAACCTTAAAGACAACGGCGGTGGCTACAGATAGAATTGCAAGCTTTCGTAACTCATGTCCGCTg                              <  2:3408258/93‑1 (MQ=255)
                                                            acacTAAACTCCATGCACTACGCTCCTGAACCTTAAAGACAACGGCGGTGGCTACAGATAGAATTGCAAGCTTTCGTAACTCATGTCCGCTGt                             <  1:4960896/93‑1 (MQ=255)
                                                            acacTAAACTCCATGCACTACGCTCCTGAACCTTAAAGACAACGGCGGTGGCTACAGATAGAATTGCAAGCTTTCGTAACTCATGTCCGCTGt                             <  1:221109/93‑1 (MQ=255)
                                                            acacTAAACTCCATGCACTACGCTCCTGAACCTTAAAGACAACGGCGGTGGCTACAGATAGAATTGCAAGCTTTCGTAACTCATGTCCGCTGt                             <  2:5925398/93‑1 (MQ=255)
                                                             cacTAAACTCCATGCACTACGCTCCTGAACCTTAAAGACAACGGCGGTGGCTACAGATAGAATTGCAAGCTTTCGTAACTCATGTCCGCTGtt                            <  1:4893363/93‑1 (MQ=255)
                                                              acTAAACTCCATGCACTACGCTCCTGAACCTTAAAGACAACGGCGGTGGCTACAGATAGAATTGCAAGCTTTCGTAACTCATGTCCGCTGTTg                           <  2:2465342/93‑1 (MQ=255)
                                                                 aaaCTCCATGCACTACGCTCCTGAACCTTAAAGACAACGGCGGTGGCTACAGATAGAATTGCAAGCTTTCGTAACTCATGTCCGCTGTTGCGa                        <  2:5007797/93‑1 (MQ=255)
                                                                  aaCTCCATGCACTACGCTCCTGAACCTTAAAGACAACGGCGGTGGCTACAGATAGAATTGCAAGCTTTCGTAACTCATGTCCGCTGTTGCGAt                       <  1:3806981/93‑1 (MQ=255)
                                                                  aaCTCCATGCACTACGCTCCTGAACCTTAAAGACAACGGCGGTGGCTACAGATAGAATTGCAAGCTTTCGTAACTCATGTCCGCTGTTGCGAt                       >  2:1009449/1‑93 (MQ=255)
                                                                   aCTCCATGCACTACGCTCCTGAACCTTAAAGACAACGGCGGTGGCTACAGATAGAATTGCAAGCTTTCGTAACTCATGTCCGCTGTTGCGATg                      <  1:1650580/93‑1 (MQ=255)
                                                                     tCCATGCACTACGCTCCTGAACCTTAAAGACAACGGCGGTGGCTACAGATAGAATTGCAAGCTTTCGTAACTCATGTCCGCTGTTGCGATGAc                    >  2:4198334/1‑93 (MQ=255)
                                                                      ccATGCACTACGCTCCTGAACCTTAAAGACAACGGCGGTGGCTACAGATAGAATTGCAAGCTTTCGTAACTCATGTCCGCTGTTGCGATGACt                   >  1:3854121/1‑93 (MQ=255)
                                                                       cATGCACTACGCTCCTGAACCTTAAAGACAACGGCGGTGGCTACAGATAGAATTGCAAGCTTTCGTAACTCATGTCCGCTGTTGCGATGACtt                  <  2:5213086/93‑1 (MQ=255)
                                                                        aTGCACTACGCTCCTGAACCTTAAAGACAACGGCGGTGGCTACAGATAGAATTGCAAGCTTTCGTAACTCATGTCCGCTGTTGCGATGACTTc                 <  2:5467435/93‑1 (MQ=255)
                                                                        aTGCACTACGCTCCTGAACCTTAAAGACAACGGCGGTGGCTACAGATAGAATTGCAAGCTTTCGTAACTCATGTCCGCTGTTGCGATGACTTc                 >  2:211354/1‑93 (MQ=255)
                                                                          gCACTACGCTCCTGAACCTTAAAGACAACGGCGGCGGCTACAGATAGAATTGCAAGCTTTCGTAACTCATGTCCGCTGTTGCGATGACTTCgt               >  1:3139108/1‑93 (MQ=255)
                                                                            aCTACGCTCCTGAACCTTAAAGACAACGGCGGTGGCTACAGATAGAATTGCAAGCTTTCGTAACTCATGTCCGCTGTTGCGATGACTTCgtgt             <  2:3319968/93‑1 (MQ=255)
                                                                            aCTACGCTCCTGAACCTTAAAGACAACGGCGGTGGCTACAGATAGAATTGCAAGCTTTCGTAACTCATGTCCGCTGTTGCGATGACTTCgtgt             <  2:5382422/93‑1 (MQ=255)
                                                                            aCTACGCTCCTGAACCTTAAAGACAACGGCGGTGGCTACAGATAGAATTGCAAGCTTTCGTAACTCATGTCCGCTGTTGCGATGACTTCgtgt             >  2:6564496/1‑93 (MQ=255)
                                                                              tACGCTCCTGAACCTTAAAGACAACGGCGGTGGCTACAGATAGAATTGCAAGCTTTCGTAACTCATGTCCGCTGTTGCGATGACTTCGTGTTa           >  1:4513908/1‑93 (MQ=255)
                                                                                 gCTCCTGAACCTTAAAGACAACGGCGGTGGCTACAGATAGAATTGCAAGCTTTCGTAACTCATGTCCGCTGTTGCGATGACTTCGTGTTAATc        >  1:5880218/1‑93 (MQ=255)
                                                                                  cTCCTGAACCTTAAAGACAACGGCGGTGGCTACAGATAGAATTGCAAGCTTTCGTAACTCATGTCCGCTGTTGCGATGACTTCGTGTTAATCt       >  2:6519447/1‑93 (MQ=255)
                                                                                   tCCTGAACCTTAAAGACAACGGCGGTGGCTACAGATAGAATTGCAAGCTTTCGTAACTCATGTCCGCTGTTGCGATGACTTCGTGTTAATCtt      >  1:5534190/1‑93 (MQ=255)
                                                                                    ccTGAACCTTAAAGACAACGGCGGTGGCTACAGATAGAATTGCAAGCTTTCGTAACTCATGTCCGCTGTTGCGATGACTTCGTGTTAATCTTa     >  2:838486/1‑93 (MQ=255)
                                                                                     cTGAACCTTAAAGACAACGGCGGTGGCTACAGATAGAATTGCAAGCTTTCGTAACTCATGTCCGCTGTTGCGATGACTTCGTGTTAATCTTaa    >  2:403276/1‑93 (MQ=255)
                                                                                     cTGAACCTTAAAGACAACGGCGGTGGCTACAGATAGAATTGCAAGCTTTCGTAACTCATGTCCGCTGTTGCGATGACTTCGTGTTAATCTTaa    >  1:1021581/1‑93 (MQ=255)
                                                                                     cTGAACCTTAAAGACAACGGCGGTGGCTACAGATAGAATTGCAAGCTTTCGTAACTCATGTCCGCTGTTGCGATGACTTCGTGTTAATCTTaa    <  1:5624680/93‑1 (MQ=255)
                                                                                      tGAACCTTAAAGACAACGGCGGTGGCTACAGATAGAATTGCAAGCTTTCGTAACTCATGTCCGCTGTTGCGATGACTTCGTGTTAATCTTAAc   <  2:467782/93‑1 (MQ=255)
                                                                                       gAACCTTAAAGACAACGGCGGTGGCTACAGATAGAATTGCAAGCTTTCGTAACTCATGTCCGCTGTTGCGATGACTTCGTGTTAATCTTAACg  >  2:4808552/1‑93 (MQ=255)
                                                                                          |                                                                                         
GGTTCGAAGACGCCCACGACGATGCAGGCACTCCGCTGTTTCTCCGGGCTACCGCTTTTTACACTAAACTCCATGCACTACGCTCCTGAATCTTAAAGACAACGGCGGTGGCTACAGATAGAATTGCAAGCTTTCGTAACTCATGTCCGCTGTTGCGATGACTTCGTGTTAATCTTAACG  >  NC_000913/4483446‑4483625

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑