BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,511,365	A→G	L173P (CTT→CCT)	fecB ←	FecB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,511,365	0	A	G	99.6%	882.6 / ‑4.9	275	L173P (CTT→CCT)	fecB	FecB
Reads supporting (aligned to +/- strand): ref base A (1/0); new base G (119/155); total (120/155)
Fisher's exact test for biased strand distribution p-value = 4.36e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.83e-01

Only 100 of 286 total aligned reads displayed.

GCCAGCACGCTGCCGGTCCAGGTCTCCTGAGTATGCAGGTTGAATTGCTGTTCGCGTGATGTGCCAAAGGCCACGCGTGTCCCTTTGGGAAGCTGGCTGGCCCACTGCGCCATCCTCTCTTTATGTTGTTCCAGACGTGCCTGCATCTCTCGCTTTTTACCCACCATTTCGCCGATGATA  >  NC_000913/4511275‑4511454

   aGCACGCTGCCGGTCCAGGTCTCCTGAGTATGCAGGTTGAATTGCTGTTCGCGTGATGTGCCAAAGGCCACGCGTGTCCCTTTGGGAGgctgg                                                                                      >  1:352695/1‑93 (MQ=255)

    gCACGCTGCCGGTCCAGGTCTCCTGAGTATGCAGGTTGAATTGCTGTTCGCGTGATGTGCCAAAGGCCACGCGTGTCCCTTTGGGAGgctggc                                                                                     <  1:516121/93‑1 (MQ=255)

    gCACGCTGCCGGTCCAGGTCTCCTGAGTATGCAGGTTGAATTGCTGTTCGCGTGATGTGCCAAAGGCCACGCGTGTCCCTTTGGGAGgctggc                                                                                     >  2:1133896/1‑93 (MQ=255)

     cgctcTGCCGGTCCAGGTCTCCTGAGTATGCAGGTTGAATTGCTGTTCGCGTGATGTGCCAAAGGCCACGCGTGTCCCTTTGGGAggctggct                                                                                    <  2:1930764/89‑1 (MQ=255)

     cACGCTGCCGGTCCAGGTCTCCTGAGTATGCAGGTTGAATTGCTGTTCGCGTGATGTGCCAAAGGCCACGCGTGTCCCTTTGGGAggctggct                                                                                    >  1:629097/1‑93 (MQ=255)

       cGCTGCCGGTCCAGGTCTCCTGAGTATGCAGGTTGAATTGCTGTTCGCGTGATGTGCCAAAGGCCACGCGTGTCCCTTTGGGAGGctggctgg                                                                                  <  1:947640/93‑1 (MQ=255)

         cTGCCGGTCCAGGTCTCCTGAGTATGCAGGTTGAATTGCTGTTCGCGCGATGTGCCAAAGGCCACGCGTGTCCCTTTGGGAGGCTGGCTGGcc                                                                                <  2:4322150/93‑1 (MQ=255)

           gCCGGTCCAGGTCTCCTGAGTATGCAGGTTGAATTGCTGTTCGCGTGATGTGCCAAAGGCCACGCGTGTCCCTTTGGGAGGCTGGCTGGCCCa                                                                              <  1:6438950/93‑1 (MQ=255)

            ccGGTCCAGGTCTCCTGAGTATGCAGGTTGAATTGCTGTTCGCGTGATGTGCCAAAGGCCACGCGTGTCCCTTTGGGAGGCTGGCTGGCCCAc                                                                             <  2:183047/93‑1 (MQ=255)

             cGGTCCAGGTCTCCTGAGTATGCAGGTTGAATTGCTGTTCGCGTGATGTGCCAAAGGCCACGCGTGTCCCTTTGGGAGGCTGGCTGGCCCACt                                                                            <  1:1064442/93‑1 (MQ=255)

             cGGTCCAGGTCTCCTGAGTATGCAGGTTGAATTGCTGTTCGCGTGATGTGCCAAAGGCCACGCGTGTCCCTTTGGGAGGCTGGCTGGCCCACt                                                                            <  2:1668066/93‑1 (MQ=255)

              ggTCCAGGTCTCCTGAGTATGCAGGTTGAATTGCTGTTCGCGTGATGTGCCAAAGGCCACGCGTGTCCCTTTGGGAGGCTGGCTGGCCCACTg                                                                           <  2:4093310/93‑1 (MQ=255)

               gTCCAGGTCTCCTGAGTATGCAGGTTGAATTGCTGTTCGCGTGATGTGCCAAAGGCCACGCGTGTCCCTTTGGGAGGCTGGCTGGCCCACTgc                                                                          >  2:823336/1‑93 (MQ=255)

               gTCCAGGTCTCCTGAGTATGCAGGTTGAATTGCTGTTCGCGTGATGTGCCAAAGGCCACGCGTGTCCCTTTGGGAGGCTGGCTGGCCCACTgc                                                                          <  2:4528557/93‑1 (MQ=255)

               gTCCAGGTCTCCTGAGTATGCAGGTTGAATTGCTGTTCGCGTGATGTGCCAAAGGCCACGCGTGTCCCTTTGGGAGGCTGGCTGGCCCACTgc                                                                          >  1:757303/1‑93 (MQ=255)

                tCCAGGTCTCCTGAGTATGCAGGTTGAATTGCTGTTCGCGTGATGTGCCAAAGGCCACGCGTGTCCCTTTGGGAGGCTGGCTGGCCCACTgcg                                                                         <  2:6528205/93‑1 (MQ=255)

                   aGGTCTCCTGAGTATGCAGGTTGAATTGCTGTTCGCGTGATGTGCCAAAGGCCACGCGTGTCCCTTTGGGAGGCTGGCTGGCCCACTGCGCCa                                                                      <  2:2464367/93‑1 (MQ=255)

                   aGGTCTCCTGAGTATGCAGGTTGAATTGCTGTTCGCGTGATGTGCCAAAGGCCACGCGTGTCCCTTTGGGAGGCTGGCTGGCCCACTGCGCCa                                                                      >  1:902443/1‑93 (MQ=255)

                    ggTCTCCTGAGTATGCAGGTTGAATTGCTGTTCGCGTGATGTGCCAAAGGCCACGCGTGTCCCTTTGGGAGGCTGGCTGGCCCACTGCGCCAt                                                                     >  2:3240661/1‑93 (MQ=255)

                    ggTCTCCTGAGTATGCAGGTTGAATTGCTGTTCGCGTGATGTGCCAAAGGCCACGCGTGTCCCTTTGGGAGGCTGGCTGGCCCACTGCGCCAt                                                                     >  2:4011582/1‑93 (MQ=255)

                     gTCTCCTGAGTATGCAGGTTGAATTGCTGTTCGCGTGATGTGCCAAAGGCCACGCGTGTCCCTTTGGGAGGCTGGCTGGCCCACTGCGCCATc                                                                    <  2:547411/93‑1 (MQ=255)

                        tcCTGAGTATGCAGGTTGAATTGCTGTTCGCGTGATGTGCCAAAGGCCACGCGTGTCCCTTTGGGAGGCTGGCTGGCCCACTGCGCCATCctc                                                                 <  1:2448536/93‑1 (MQ=255)

                        tcCTGAGTATGCAGGTTGAATTGCTGTTCGCGTGATGTGCCAAAGGCCACGCGTGTCCCTTTGGGAGGCTGGCTGGCCCACTGCGCCATCctc                                                                 >  2:6067440/1‑93 (MQ=255)

                         ccTGAGTATGCAGGTTGAATTGCTGTTCGCGTGATGTGCCAAAGGCCACGCGTGTCCCTTTGGGAGGCTGGCTGGCCCACTGCGCCATCctct                                                                >  1:3293485/1‑93 (MQ=255)

                         ccTGAGTATGCAGGTTGAATTGCTGTTCGCGTGATGTGCCAAAGGCCACGCGTGTCCCTTTGGGAGGCTGGCTGGCCCACTGCGCCATCctct                                                                <  1:3179302/93‑1 (MQ=255)

                           tGAGTATGCAGGTTGAATTGCTGTTCGCGTGATGTGCCAAAGGCCACGCGTGTCCCTTTGGGAGGCTGGCTGGCCCACTGCGCCATCctctct                                                              <  2:4865977/93‑1 (MQ=255)

                            gAGTATGCAGGTTGAATTGCTGTTCGCGTGATGTGCCAAAGGCCACGCGTGTCCCTTTGGGAGGCTGGCTGGCCCACTGCGCCATCCTCTCtt                                                             >  1:1235728/1‑93 (MQ=255)

                            gAGTATGCAGGTTGAATTGCTGTTCGCGTGATGTGCCAAAGGCCACGCGTGTCCCTTTGGGAGGCTGGCTGGCCCACTGCGCCATCCTCTCtt                                                             <  1:225750/93‑1 (MQ=255)

                             aGTATGCAGGTTGAATTGCTGTTCGCGTGATGTGCCAAAGGCCACGCGTGTCCCTTTGGGAGGCTGGCTGGCCCACTGCGCCATCCTCTCttt                                                            >  2:3616020/1‑93 (MQ=255)

                              gTATGCAGGTTGAATTGCTGTTCGCGTGATGTGCCAAAGGCCACGCGTGTCCCTTTGGGAGGCTGGCTGGCCCACTGCGCCATCCTCTCTTTa                                                           <  2:3851719/93‑1 (MQ=255)

                               tATGCAGGTTGAATTGCTGTTCGCGTGATGTGCCAAAGGCCACGCGTGTCCCTTTGGGAGGCTGGCTGGCCCACTGCGCCATCCTCTCTTTAt                                                          >  1:4052576/1‑93 (MQ=255)

                               tATGCAGGTTGAATTGCTGTTCGCGTGATGTGCCAAAGGCCACGCGTGTCCCTTTGGGAGGCTGGCTGGCCCACTGCGCCATCCTCTCTTTAt                                                          >  2:2746924/1‑93 (MQ=255)

                                 tGCAGGTTGAATTGCTGTTCGCGTGATGTGCCAAAGGCCACGCGTGTCCCTTTGGGAGGCTGGCTGGCCCACTGCGCCATCCTCTCTTTAtgt                                                        <  1:3945261/93‑1 (MQ=255)

                                 tGCAGGTTGAATTGCTGTTCGCGTGATGTGCCAAAGGCCACGCGTGTCCCTTTGGGAGGCTGGCTGGCCCACTGCGCCATCCTCTCTTTAtgt                                                        <  2:5297120/93‑1 (MQ=255)

                                 tGCAGGTTGAATTGCTGTTCGCGTGATGTGCCAAAGGCCACGCGTGTCCCTTTGGGAGGCTGGCTGGCCCACTGCGCCATCCTCTCTTTAtgt                                                        <  2:1199199/93‑1 (MQ=255)

                                 tGCAGGTTGAATTGCTGTTCGCGTGATGTGCCAAAGGCCACGCGTGTCCCTTTGGGAGGCTGGCTGGCCCACTGCGCCATCCTCTCTTTAtgt                                                        <  1:6428974/93‑1 (MQ=255)

                                 tGCAGGTTGAATTGCTGTTCGCGTGATGTGCCAAAGGCCACGCGTGTCCCTTTGGGAGGCTGGCTGGCCCACTGCGCCATCCTCTCTTTAtgt                                                        <  2:4030573/93‑1 (MQ=255)

                                   cAGGTTGAATTGCTGTTCGCGTGATGTGCCAAAGGCCACGCGTGTCCCTTTGGGAGGCTGGCTGGCCCACTGCGCCATCCTCTCTTTAtgttg                                                      <  2:2065403/93‑1 (MQ=255)

                                    aGGTTGAATTGCTGTTCGCGTGATGTGCCAAAGGCCACGCGTGTCCCTTTGGGAGGCTGGCTGGCCCACTGCGCCATCCTCTCTTTAtgttgt                                                     <  1:2737962/93‑1 (MQ=255)

                                     ggTTGAATTGCTGTTCGCGTGATGTGCCAAAGGCCACGCGTGTCCCTTTGGGAGGCTGGCTGGCCCACTGCGCCATCCTCTCTTTATgttgtt                                                    <  2:3454388/93‑1 (MQ=255)

                                       ttGAATTGCTGTTCGCGTGATGTGCCAAAGGCCACGCGTGTCCCTTTGGGAGGCTGGCTGGCCCACTGCGCCATCCTCTCTTTATGTTGTTcc                                                  <  1:1793960/93‑1 (MQ=255)

                                        tGAATTGCTGTTCGCGTGATGTGCCAAAGGCCACGCGTGTCCCTTTGGGAGGCTGGCTGGCCCACTGCGCCATCCTCTCTTTATGTTGTTCCa                                                 <  2:3754365/93‑1 (MQ=255)

                                         gAATTGCTGTTCGCGTGATGTGCCAAAGGCCACGCGTGTCCCTTTGGGAGGCTGGCTGGCCCACTGCGCCATCCTCTCTTTATGTTGTTCCAg                                                >  1:4949510/1‑93 (MQ=255)

                                         gAATTGCTGTTCGCGTGATGTGCCAAAGGCCACGCGTGTCCCTTTGGGAGGCTGGCTGGCCCACTGCGCCATCCTCTCTTTATGTTGTTCCAg                                                <  1:1429601/93‑1 (MQ=255)

                                          aaTTGCTGTTCGCGTGATGTGCCAAAGGCCACGCGTGTCCCTTTGGGAGGCTGGCTGGCCCATTGCGCCATCCTCTCTTTATGTTGTTCCAGa                                               >  1:2682289/1‑93 (MQ=255)

                                          aaTTGCTGTTCGCGTGATGTGCCAAAGGCCACGCGTGTCCCTTTGGGAGGCTGGCTGGCCCACTGCGCCATCCTCTCTTTATGTTGTTCCAGa                                               <  2:3070562/93‑1 (MQ=255)

                                           aTTGCTGTTCGCGTGATGTGCCAAAGGCCACGCGTGTCCCTTTGGGAGGCTGGCTGGCCCACTGCGCCATCCTCTCTTTATGTTGTTCCAGAc                                              <  2:2635782/93‑1 (MQ=255)

                                            ttGCTGTTCGCGTGATGTGCCAAAGGCCACGCGTGTCCCTTTGGGAGGCTGGCTGGCCCACTGCGCCATCCTCTCTTTATGTTGTTCCAGACg                                             >  1:2486547/1‑93 (MQ=255)

                                             tGCTGTTCGCGTGATGTGCCAAAGGCCACGCGTGTCCCTTTGGGAGGCTGGCTGGCCCACTGCGCCATCCTCTCTTTATGTTGTTCCAGACGt                                            >  1:1102357/1‑93 (MQ=255)

                                             tGCTGTTCGCGTGATGTGCCAAAGGCCACGCGTGTCCCTTTGGGAGGCTGGCTGGCCCACTGCGCCATCCTCTCTTTATGTTGTTCCAGACGt                                            <  1:3658592/93‑1 (MQ=255)

                                              gCTGTTCGCGTGATGTGCCAAAGGCCACGCGTGTCCCTTTGGGAGGCTGGCTGGCCCACTGCGCCATCCTCTCTTTATGTTGTTTCAGCCGTg                                           >  2:104477/1‑93 (MQ=255)

                                                tGTTCGCGTGATGTGCCAAAGGCCACGCGTGTCCCTTTGGGAGGCTGGCTGGCCCACTGCGCCATCCTCTCTTTATGTTGTTCCAGACGTGcc                                         <  1:6695196/93‑1 (MQ=255)

                                                tGTTCGCGTGATGTGCCAAAGGCCACGCGTGTCCCTTTGGGAGGCTGGCTGGCCCACTGCGCCATCCTCTCTTTATGTTGTTCCAGACGTGcc                                         >  1:1688301/1‑93 (MQ=255)

                                                tGTTCGCGTGATGTGCCAAAGGCCACGCGTGTCCCTTTGGGAGGCTGGCTGGCCCACTGCGCCATCCTCTCTTTATGGTGTTCCAGACGTGcc                                         >  1:4681077/1‑93 (MQ=255)

                                                tGTTCGCGTGATGTGCCAAAGGCCACGCGTGTCCCTTTGGGAGGCTGGCTGGCCCACTGCGCCATCCTCCCTTTATGTTGTTCCAGACGCGcc                                         >  1:4630711/1‑93 (MQ=255)

                                                 gtgcgcgTGATGAGCCAAAGGCCATGCGTGTCCCTTTGGGAGGCTGGCTGGCCCACTGCGCCATCCTCTCTTTATGTTGTTCCAGACGTGCCt                                        <  1:5306107/90‑1 (MQ=255)

                                                 gTTCGCGTGATGTGCCAAAGGCCACGCGTGTCCCTTTGGGAGGCTGGCTGGCCCACTGCGCCATCCTCTCTTTATGTTGTTCCAGACGTGCCt                                        <  1:1996857/93‑1 (MQ=255)

                                                  ttCGCGTGATGTGCCAAAGGCCACGCGTGTCCCTTTGGGAGGCTGGCTGGCCCACTGCGCCATCCTCTCTTTATGTTGTTCCAGACGTGCCTg                                       <  1:5813055/93‑1 (MQ=255)

                                                  ttCGCGTGATGTGCCAAAGGCCACGCGTGTCCCTTTGGGAGGCTGGCTGGCCCACTGCGCCATCCTCTCTTTATGTTGTTCCAGACGTGCCTg                                       >  1:5927063/1‑93 (MQ=255)

                                                    cgcgTGATGTGCCAAAGGCCACGCGTGTCCCTTTGGGAGGCTGGCTGGCCCACTGCGCCATCCTCTCTTTATGTTGTTCCAGACGTGCCTGCa                                     >  2:4424343/1‑93 (MQ=255)

                                                      cgTGATGTGCCAAAGGCCACGCGTGTCCCTTTGGGAGGCTGGCTGGCCCACTGCGCCATCCTCTCTTTATGTTGTTCCAGACGTGCCTGCAtc                                   <  2:3920993/93‑1 (MQ=255)

                                                       gTGATGTGCCAAAGGCCACGCGTGTCCCTTTGGGAGGCTGGCTGGCCCACTGCGCCATCCTCTCTTTATGTTGTTCCAGACGTGCCTGCAtct                                  >  1:5996709/1‑93 (MQ=255)

                                                       gTGATGTGCCAAAGGCCACGCGTGTCCCTTTGGGAGGCTGGCTGGCCCACTGCGCCATCCTCTCTTTATGTTGTTCCAGACGTGCCTGCAtct                                  >  1:2129742/1‑93 (MQ=255)

                                                        tGATGTGCCAAAGGCCACGCGTGTCCCTTTGGGAGGCTGGCTGGCCCACTGCGCCATCCTCTCTTTATGTTGTTCCAGACGTGCCTGCAtctc                                 >  1:1864306/1‑93 (MQ=255)

                                                        tGATGTGCCAAAGGCCACGCGTGTCCCTTTGGGAGGCTGGCTGGCCCACTGCGCCATCCTCTCTTTATGTTGTTCCAGACGTGCCTGCAtctc                                 <  2:1268414/93‑1 (MQ=255)

                                                         gATGTGCCAAAGGCCACGCGTGTCCCTTTGGGAGGCTGGCTGGCCCACTGCGCCATCCTCTCTTTATGTTGTTCCAGACGTGCCTGCAtctct                                >  2:6324763/1‑93 (MQ=255)

                                                         gATGTGCCAAAGGCCACGCGTGTCCCTTTGGGAGGCTGGCTGGCCCACTGCGCCATCCTCTCTTTATGTTGTTCCAGACGTGCCTGCAtctct                                <  1:2171930/93‑1 (MQ=255)

                                                          aggtgCCAAAGGCCACGCGTGTCCCTTTGGGAGGCTGGCTGGCCCACTGCGCCATCCTCTCTTTATGTTGTTCCAGACGTGCCTGCAtctctc                               <  2:6013599/91‑1 (MQ=255)

                                                          aTGTGCCAAAGGCCACGCGTGTCCCTTTGGGAGGCTGGCTGGCCCACTGCGCCATCCTCTCTTTATGTTGTTCCAGACGTGCCTGCAtctctc                               >  2:6428502/1‑93 (MQ=255)

                                                            gtgCCAAAGGCCACGCGTGTCCCTTTGGGAGGCTGGCTGGCCCACTGCGCCATCCTCTCTTTATGTTGTTCCAGACGTGCCTGCCTCTCTCGc                             >  1:6250179/1‑93 (MQ=255)

                                                             tgCCAAAGGCCACGCGTGTCCCTTTGGGAGGCTGGCTGGCCCACTGCGCCATCCTCTCTTTATGTTGTTCCAGACGTGCCTGCATCTCTCGCt                            >  2:2524338/1‑93 (MQ=255)

                                                             tgCCAAAGGCCACGCGTGTCCCTTTGGGAGGCTGGCTGGCCCACTGCGCCATCCTCTCTTTATGTTGTTCCAGACGTGCCTGCATCTCTCGCt                            <  1:238143/93‑1 (MQ=255)

                                                              gcaaaaGGCCACGCGTTTCCCTTTGGGAGGCTGGCTGGCCCACTGCGCCATCCTCTCTTTATGTTGTTCCAGACGTGCCTGCATCTCTCGCtt                           <  1:6497245/90‑1 (MQ=255)

                                                              gCCAAAGGCCACGCGTGTCCCTTTGGGAGGCTGGCTGGCCCACTGCGCCATCCTCTCTTTATGTTGTTCCAGACGTGCCTGCATCTCTCGCtt                           <  2:2182184/93‑1 (MQ=255)

                                                               ccAAAGGCCACGCGTGTCCCTTTGGGAGGCTGGCTGGCCCACTGCGCCATCCTCTCTTTATGTTGTTCCAGACGTGCCTGCATCTCTCGCttt                          <  2:3319257/93‑1 (MQ=255)

                                                                cAAAGGCCACGCGTGTCCCTTTGGGAGGCTGGCTGGCCCACTGCGCCATCCTCTCTTTATGTTGTTCCAGACGTGCCTGCATCTCTCGCtttt                         >  2:5572954/1‑93 (MQ=255)

                                                                 caaGGCCACGCGTGTCCCTTTGGGAGGCTGGCTGGCCCACTGCGCCATCCTCTCTTTATGTTGTTCCAGACGTGCCTGCATCTCTCGCttttt                        <  2:985442/92‑1 (MQ=255)

                                                                 aaaGGCCACGCGTGTCCCTTTGGGAGGCTGGCTGGCCCACTGCGCCATCCTCTCTTTATGTTGTTCCAGACGTGCCTGCATCTCTCGCttttt                        <  2:3370951/93‑1 (MQ=255)

                                                                 aaaGGCCACGCGTGTCCCTTTGGGAGGCTGGCTGGCCCACTGCGCCATCCTCTCTTTATGTTGTTCCAGACGTGCCTGCATCTCTCGCttttt                        >  1:1467808/1‑93 (MQ=255)

                                                                 aaaGGCCACGCGTGTCCCTTTGGGAGGCTGGCTGGCCCACTGCGCCATCCTCTCTTTATGTTGTTCCAGACGTGCCTGCATCTCTCGCttttt                        <  2:1499427/93‑1 (MQ=255)

                                                                   aGGCCACGCGTGTCCCTTTGGGAGGCTGGCTGGCCCACTGCGCCATCCTCTCTTTATGTTGTTCCAGACGTGCCTGCATCTCTCGCTTTTTAc                      >  2:1026861/1‑93 (MQ=255)

                                                                    ggCCACGCGTGTCCCTTTGGGAGGCTGGCTGGCCCACTGCGCCATCCTCTCTTTATGTTGTTCCAGACGTGCCTGCATCTCTCGCTTTTTAcc                     >  1:53655/1‑93 (MQ=255)

                                                                      ccACGCGTGTCCCTTTGGGAGGCTGGCTGGCCCACTGCGCCATCCTCTCTTTATGTTGTTCCAGACGTGCCTGCATCTCTCGCTGTTTACcca                   <  1:6530613/93‑1 (MQ=255)

                                                                      ccACGCGTGTCCCTTTGGGAGGCTGGCTGGCCCACTGCGCCATCCTCTCTTTATGTTGTTCCAGACGTGCCTGCATCTCTCCCTTTTTACcca                   >  2:4666679/1‑93 (MQ=255)

                                                                       cACGCGTGTCCCTTTGGGAGGCTGGCTGGCCCACTGCGCCATCCTCTCTTTATGTTGTTCCAGACGTGCCTGCATCTCTCGCTTTTTACccac                  >  1:204440/1‑93 (MQ=255)

                                                                        ccgcgTGTCCCTTTGGGAGGCTGGCTGGCCCACTGCGCCATCCTCTCTTTATGTTGTTCCAGACGTGCCTGCATCTCTCGCTTTTTACccacc                 <  2:697550/92‑1 (MQ=255)

                                                                           cgTGTCCCTTTGGGAGGCTGGCTGGCCCACTGCGCCATCCTCTCTTTATGTTGTTCCAGACGTGCCTGCATCTCTCGCTTTTTACCCACCAtt              <  1:5673568/93‑1 (MQ=255)

                                                                             tgtCCCTTTGGGAGGCTGGCTGGCCCACTGCGCCATCCTCTCTTTATGTTGTTCCAGACGTGCCTGCATCTCTCGCTTTTTACCCACCATTTc            <  2:234232/93‑1 (MQ=255)

                                                                             tgtCCCTTTGGGAGGCTGGCTGGCCCACTGCGCCATCCTCTCTTTATGTTGTTCCAGACGTGCCTGCATCTCTCGCTTTTTACCCACCATTTc            <  2:3283233/93‑1 (MQ=255)

                                                                             tgtCCCTTTGGGAGGCTGGCTGGCCCACTGCGCCATCCTCTCTTTATGTTGTTCCAGACGTGCCTGCATCTCTCGCTTTTTACCCACCATTTc            <  2:5007742/93‑1 (MQ=255)

                                                                             tgtCCCTTTGGGAGGCTGGCTGGCCCACTGCGCCATCCTCTCTTTATGTTGTTCCAGACGTGCCTGCATCTCTCGCTTTTTACCCACCATTTc            <  1:341403/93‑1 (MQ=255)

                                                                              gtCCCTTTGGGAGGCTGGCTGGCCCACTGCGCCATCCTCTCTTTATGTTGTTCCAGACGTGCCTGCATCTCTCGCTTTTTACCCACCATTTCg           <  2:2270317/93‑1 (MQ=255)

                                                                              gtCCCTTTGGGAAGCTGGCTGGCCCACTGCGCCATCCTCTCTTTATGTTGTTCCAGACGTGCCTGCATCTCTCGCTTTTTACCCACCATTTCg           >  1:2817524/1‑93 (MQ=255)

                                                                                cccTTTGGGAGGCTGGCTGGCCCACTGCGCCATCCTCTCTTTATGTTGTTCCAGACGTGCCTGCATCTCTCGCTTTTTACCCACCATTTCGcc         <  2:4787700/93‑1 (MQ=255)

                                                                                  cTTTGGGAGGCTGGCTGGCCCACTGCGCCATCCTCTCTTTATGTTGTTCCAGACGTGCCTGCATCTCTCGCTTTTTACCCACCATTTCGCCga       >  1:567698/1‑93 (MQ=255)

                                                                                   tttGGGAGGCTGGCTGGCCCACTGCGCCATCCTCTCTTTATGTTGTTCCACACGTGCCTGCATCTCTCGCTTTTTACCCACCATTTCGCCgat      <  2:3567850/93‑1 (MQ=255)

                                                                                      gggAGGCTGGCTGGCCCACTGCGCCATCCTCTCTTTATGTTGTTCCAGACGTGCCTGCATCTCTCGCTTTTTACCCACCATTTCGCCgatgat   <  1:4259594/93‑1 (MQ=255)

                                                                                      gggAGGCTGGCTGGCCCACTGCGCCATCCTCTCTTTATGTTGTTCCAGACGTGCCTGCATCTCTCGCTTTTTACCCACCATTTCGCCgatgat   <  2:5783216/93‑1 (MQ=255)

                                                                                      gggAGGCTGGCTGGCCCACTGCGCCATCCTCTCTTTATGTTGTTCCAGACGTGCCTGCATCTCTCGCTTTTTACCCACCATTTCGCCgatgat   >  1:3016255/1‑93 (MQ=255)

                                                                                       ggAGGCTGGCTGGCCCACTGCGCCATCCTCTCTTTATGTTGTTCCAGACGTGCCTGCATCTCTCGCTTTTTACCCACCATTTCGCCGATGATa  <  1:4744703/93‑1 (MQ=255)

GCCAGCACGCTGCCGGTCCAGGTCTCCTGAGTATGCAGGTTGAATTGCTGTTCGCGTGATGTGCCAAAGGCCACGCGTGTCCCTTTGGGAAGCTGGCTGGCCCACTGCGCCATCCTCTCTTTATGTTGTTCCAGACGTGCCTGCATCTCTCGCTTTTTACCCACCATTTCGCCGATGATA  >  NC_000913/4511275‑4511454

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 19 ≤` `ATCG/ATCG` `< 28 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`