BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,551,614	T→C	L382P (CTG→CCG)	uxuB →	D‑mannonate oxidoreductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,551,614	0	T	C	100.0%	873.8 / NA	269	L382P (CTG→CCG)	uxuB	D‑mannonate oxidoreductase
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (158/111); total (158/111)

Only 100 of 285 total aligned reads displayed.

CGCTGCTGATCGAGCGTTTCAGCAACCCGTCTCTGCGTCACCGTACCTGGCAGATTGCGATGGACGGCAGCCAGAAGTTACCGCAGCGTCTGCTGGACCCGGTGCGTCTGCACCTGCAAAACGGCGGCAGCTGGCGTCACCTGGCGCTGGGCGTGGCTGGCTGGATGCGTTACACCCAGG  >  NC_000913/4551524‑4551703

cGCTGCTGATCGAGCGTTTCAGCAACCCGTCTCTGCGTCACCGTACCTGGCAGATTGCGATGGACGGCAGCCAGAAGTTACCGCAGCGTccgc                                                                                         <  2:4409712/93‑1 (MQ=255)

cGCTGCTGATCGAGCGTTTCAGCAACCCGTCTCTGCGTCACCGTACCTGGCAGATTGCGATGGACGGCAGCCAGAAGTTACCGCAGCGTccgc                                                                                         <  2:5923996/93‑1 (MQ=255)

 gctgctGATCGAGCGTTTCAGCAACCCGTCTCTGCGTCACCGTACCTGGCAGATTGCGATGGACGGCAGCCAGAAGTTACCGCAGCGTccgct                                                                                        <  2:4505811/93‑1 (MQ=255)

 gctgctGATCGAGCGTTTCAGCAACCCGTCTCTGCGTCACCGTACCTGGCAGATTGCGATGGACGGCAGCCAGAAGTTACCGCAGCGTccgct                                                                                        <  2:6025519/93‑1 (MQ=255)

   tgctgATCGAGCGTTTCAGCAACCCGTCTCTGCGTCACCGTACCTGGCAGATTGCGATGGACGGCAGCCAGAAGTTACCGCAGCGTCCGCTgg                                                                                      >  1:2881424/1‑93 (MQ=255)

   tgctgATCGAGCGTTTCAGCAACCCGTCTCTGCGTCACCGTACCTGGCAGATTGCGATGGACGGCAGCCAGAAGTTACCGCAGCGTCCGCTgg                                                                                      <  2:6070403/93‑1 (MQ=255)

    gctgATCGAGCGTTTCAGCAACCCGTCTCTGCGTCACCGTACCTGGCAGATTGCGATGGACGGCAGCCAGAAGTTACCGCAGCGTCTGCTGGa                                                                                     >  1:2273143/1‑93 (MQ=255)

       gATCGAGCGTTTCAGCAACCCGTCTCTGCGTCACCGTACCTGGCAGATTGCGATGGACGGCAGCCAGAAGTTACCGCAGCGTCCGCTGGAccc                                                                                  >  2:2292861/1‑93 (MQ=255)

         tCGAGCGTTTCAGCAACCCGTCTCTGCGTCACCGTACCTGGCAGATTGCGATGGACGGCAGCCAGAAGTTACCGCAGCGTCCGCTGGACCCgg                                                                                <  1:5394210/93‑1 (MQ=255)

         tCGAGCGTTTCAGCAACCCGTCTCTGCGTCACCGTACCTGGCAGATTGCGATGGACGGCAGCCAGAAGTTACCGCAGCGTCCGCTGGACCCgg                                                                                <  2:1405535/93‑1 (MQ=255)

         tCGAGCGTTTCAGCAACCCGTCTCTGCGGCACCGTACCTGGCAGATTGCGATGGACGGCAGCCAGAAGTTACCGCAGCGTCCGCTGGACCCgg                                                                                <  2:3127347/93‑1 (MQ=255)

          cGAGCGTTTCAGCAACCCGTCTCTGCGTCACCGTACCTGGCAGATTGCGATGGACGGCAGCCAGAAGTTACCGCAGCGTCCGCTGGACCCGGt                                                                               >  1:5005245/1‑93 (MQ=255)

             gCGTTTCAGCAACCCGTCTCTGCGTCACCGTACCTGGCAGATTGCGATGGACGGCAGCCAGAAGTTACCGCAGCGTCCGCTGGACCCGGTGCg                                                                            >  2:2605301/1‑93 (MQ=255)

             gCGTTTCAGCAACCCGTCTCTGCGTCACCGTACCTGGCAGATTGCGATGGACGGCAGCCAGAAGTTACCGCAGCGTCCGCTGGACCCGGTGCg                                                                            >  1:1088090/1‑93 (MQ=255)

               gTTTCAGCAACCCGTCTCTGCGTCACCGTACCTGGCAGATTGCGATGGACGGCAGCCAGAAGTTACCGCAGCGTCCGCTGGAGCCGGTGCGTc                                                                          >  1:638142/1‑93 (MQ=255)

                tttCAGCAACCCGTCTCTGCGTCACCGTACCTGGCAGATTGCGATGGACGGCAGCCAGAAGTTACCGCAGCGTCCGCTGGACCCGGTGCGTCt                                                                         >  1:2825861/1‑93 (MQ=255)

                  tCAGCAACCCGTCTCTGCGTCACCGTACCTGGCAGATTGCGATGGACGGCAGCCAGAAGTTACCGCAGCGTCCGCTGGACCCGGGGCGTCTGc                                                                       >  2:48556/1‑93 (MQ=255)

                    aGCAACCCGTCTCTGCGTCACCGTACCTGGCAGATTGCGATGGACGGCAGCCAGAAGTTACCGCAGCGTCCGCTGGACCCGGTGCGTCTGCAc                                                                     <  1:676532/93‑1 (MQ=255)

                    aGCAACCCGTCTCTGCGTCACCGTACCTGGCAGATTGCGATGGACGGCAGCCAGAAGTTACCGCAGCGTCCGCTGGACCCGGTGCGTCTGCAc                                                                     <  1:1239125/93‑1 (MQ=255)

                     gCAACCCGTCTCTGCGTCACCGTACCTGGCAGATTGCGATGGACGGCAGCCAGAAGTTACCGCAGCGTCCGCTGGACCCGGTGCGTCTGCAcc                                                                    >  1:5190517/1‑93 (MQ=255)

                       aaCCCGTCTCTGCGTCACCGTACCTGGCAGATTGCGATGGACGGCAGCCAGAAGTTACCGCAGCGTCCGCTGGACCCGGTGCGTCTGCACCTg                                                                  >  1:1011934/1‑93 (MQ=255)

                       aaCCCGTCTCTGCGTCACCGTACCTGGCAGATTGCGATGGACGGCAGCCAGAAGTTACCGCAGCGTCCGCTGGACCCGGTGCGTCTGCACCTg                                                                  <  2:6294585/93‑1 (MQ=255)

                         cccGTCTCTGCGTCACCGTACCTGGCAGATTGCGATGGACGGCAGCCAGAAGTTACCGCAGCGTCCGCTGGACCCGGTGCGTCTGCACCTGCa                                                                <  2:6715603/93‑1 (MQ=255)

                         cccGTCTCTGCGTCACCGTACCTGGCAGATTGCGATGGACGGCAGCCAGAAGTTACCGCAGCGTCCGCTGGACCCGGTGCGTCTGCACCTGCa                                                                >  2:3242424/1‑93 (MQ=255)

                          ccGTCTCTGCGTCACCGTACCTGGCAGATTGCGATGGACGGCAGCCAGAAGTTACCGCAGCGTCCGCTGGACCCGGTGCGTCTGCACCTGCaa                                                               >  2:5909168/1‑93 (MQ=255)

                           cGTCTCTGCGTCACCGTACCTGGCAGATTGCGATGGACGGCAGCCAGAAGTTACCGCAGCGTCCGCTGGACCCGGTGCGTCTGCACCTGCaaa                                                              >  2:2143138/1‑93 (MQ=255)

                           cGTCTCTGCGTCACCGTACCTGGCAGATTGCGATGGACGGCAGCCAGAAGTTACCGCAGCGTCCGCTGGACCCGGTGCGTCTGCACCTGCaaa                                                              >  1:2302953/1‑93 (MQ=255)

                           cGTCTCTGCGTCACCGTACCTGGCAGATTGCGATGGACGGCAGCCAGAAGTTACCGCAGCGTCCGCTGGACCCGGTGCGTCTGCACCTGCaaa                                                              >  2:2516818/1‑93 (MQ=255)

                             tctcTGCGTCACCGTACCTGGCAGATTGCGATGGACGGCAGCCAGAAGTTACCGCAGCGTCCGCTGGACCCGGTGCGTCTGCACCTGCAAAAc                                                            >  1:4895810/1‑93 (MQ=255)

                             tctcTGCGTCACCGTACCTGGCAGATTGCGATGGACGGCAGCCAGAAGTTACCGCAGCGTCCGCTGGACCCGGTGCGTCTGCACCTGCAAAAc                                                            >  1:6463343/1‑93 (MQ=255)

                               tctGCGTCACCGTACCTGGCAGATTGCGATGGACGGCAGCCAGAAGTTACCGCAGCGTCCGCTGGACCCGGTGCGTCTGCACCTGCAAAAcgg                                                          >  1:6009226/1‑93 (MQ=255)

                                ctGCGTCACCGTACCTGGCAGATTGCGATGGACGGCAGCCAGAAGTTACCGCAGCGTCCGCTGGACCCGGTGCGTCTGCACCTGCCAAAcggc                                                         >  1:5276625/1‑93 (MQ=255)

                                ctGCGTCACCGTACCTGGCAGATTGCGATGGACGGCAGCCAGAAGTTACCGCAGCGTCCGCTGGACCCGGTGCGTCTGCACCTGCAAAAcggc                                                         <  2:4646801/93‑1 (MQ=255)

                                 tGCGTCACCGTACCTGGCAGATTGCGATGGACGGCAGCCAGAAGTTACCGCAGCGTCCGCTGGACCCGGTGCGTCTGCACCTGCAAAAcggcg                                                        >  2:6634325/1‑93 (MQ=255)

                                  gCGTCACCGTACCTGGCAGATTGCGATGGACGGCAGCCAGAAGTTACCGCAGCGTCCGCTGGACCCGGTGCGTCTGCACCTGCAAAAcggcgg                                                       >  2:3687412/1‑93 (MQ=255)

                                  gCGTCACCGTACCTGGCAGATTGCGATGGACGGAAGCCAGAAGTTACCGCAGCGTCCGCTGGACCCGGTGCGTCTGCACCTGCAAACcggcgg                                                       >  1:1774062/1‑93 (MQ=255)

                                  gCGTCACCGTACCTGGCAGAGTGCGATGGACGGCAGCCAGAAGTTACCGCAGCGTCCGCTGGACCCGGTGCGTCTGCACCTGCAAAAcggcgg                                                       <  2:3851636/93‑1 (MQ=255)

                                      cACCGTACCGGGCAGATTGCGATGGACGGCAGCCAGAAGTTACCGCAGCGTCCGCTGGACCCGGTGCGTCTGCACCTGCAAAACGGCGGCAGc                                                   <  1:395881/93‑1 (MQ=255)

                                        ccGTACCTGGCAGATTGCGATGGACGGCAGCCAGAAGTTACCGCAGCGTCCGCTGGACCCGGTGCGTCTGCACCTGCAAAACGGCGGCAGCTg                                                 >  1:3807772/1‑93 (MQ=255)

                                         cGTACCTGGCAGATTGCGATGGACGGCAGCCAGAAGTTACCGCAGCGTCCGCTGGACCCGGTGCGTCTGCACCTGCAAAACGGCGGCAGCTgg                                                <  1:555005/93‑1 (MQ=255)

                                         cGTACCTGGCAGATTGCGATGGACGGCAGCCAGAAGTTACCGCAGCGTCCGCTGGACCCGGTGCGTCTGCACCTGCAAAACGGCGGCAGCTgg                                                <  2:3875376/93‑1 (MQ=255)

                                         cGTACCTGGCAGATGGCGATGGACGGCAGCCAGAAGTTACCGCATCGTACGCTGGACCCGGTGCGTCTGCACCTGCAAAACGGCGGCAGCTgg                                                <  2:4120576/93‑1 (MQ=255)

                                           tACCTGGCAGATTGCGATGGACGGCAGCCAGAAGTTACCGCAGCGTCCGCTGGACCCGGTGCGTCTGCACCTGCAAAACGGCGGCAGCTGGCg                                              >  2:3090944/1‑93 (MQ=255)

                                           tACCTGGCAGATTGCGATGGACGGCAGCCAGAAGTTACCGCAGCGTCCGCTGGACCCGGTGCGTCTGCACCTGCAAAACGGCGGCAGCTGGCg                                              >  2:2763610/1‑93 (MQ=255)

                                            aCCTGGCAGATTGCGATGGACGGCAGCCAGAAGTTACCGCAGCGTCCGCTGGACCCGGTGCGTCTGCACCTGCAAAACGGCGGCAGCTGGCGt                                             <  1:5521158/93‑1 (MQ=255)

                                              cTGGCAGATTGCGATGGACGGCAGCCAGAAGTTACCGCAGCGTCCGCTGGACCCGGTGCGTCTGCACCTGCAAAACGGCGGCAGCTGGCGTcc                                           >  1:837672/1‑92 (MQ=255)

                                               tGGCAGATTGCGATGGACGGCAGCCAGAAGTTACCGCAGCGTCCGCTGGACCCGGTGCGTCTGCACCTGCAAAACGGCGGCAGCTGGCGTCAc                                          >  1:296954/1‑93 (MQ=255)

                                               tGGCAGATTGCGATGGACGGCAGCCAGAAGTTACCGCAGCGTCCGCTGGACCCGGTGCGTCTGCACCTGCAAAACGGCGGCAGCTGGCGTCAc                                          >  1:3486961/1‑93 (MQ=255)

                                                ggCAGATTGCGATGGACGGCAGCCAGAAGTTACCGCAGCGTCCGCTGGACCCGGTGCGTCTGCACCTGCAAAACGGCGGCAGCTGGCGTCAcc                                         <  2:5211316/93‑1 (MQ=255)

                                                ggCAGATTGCGATGGACGGCAGCCAGAAGTTACCGCAGCGTCCGCTGGACCCGGTGCGTCTGCACCTGCAAAACGGCGGCAGCTGGCGTCAcc                                         <  2:932515/93‑1 (MQ=255)

                                                 gCAGATTGCGATGGACGGCAGCCAGAAGTTACCGCAGCGTCCGCTGGACCCGGTGCGTCTGCACCTGCAAAACGGCGGCAGCTGGCGTCACCt                                        >  2:4236607/1‑93 (MQ=255)

                                                  cAGATTGCGATGGACGGCAGCCAGAAGTTACCGCAGCGTCCGCTGGACCCGGTGCGTCTGCACCTGCAAAACGGCGGCAGCTGGCGTCACCTg                                       <  2:2631135/93‑1 (MQ=255)

                                                   aGATTGCAATGGACGGCAGCCAGAAGTTACCGCAGCGTCCGCTGGACCCGGTGCGTCTGCACCTGCAAAACGGCGGCAGCTGGCGTCACCTgg                                      <  1:3997990/93‑1 (MQ=255)

                                                    gATTGCGATGGACGGCAGCCAGAAGTTACCGCAGCGTCCGCTGGACCCGGTGCGTCTGCACCTGCAAAACGGCGGCAGCTGGCGTCACCTGgc                                     >  1:4624582/1‑93 (MQ=255)

                                                    gATTGCGATGGACGGCAGCCAGAAGTTACCGCAGCGTCCGCTGGACCCGGTGCGTCTGCACCTGCAAAACGGCGGCAGCTGGCGTCACCTGgc                                     >  2:5578234/1‑93 (MQ=255)

                                                     aTTGCGATGGACGGCAGCCAGAAGTTACCGCAGCGTCCGCTGGACCCGGTGCGTCTGCACCTGCAAAACGGCGGCAGCTGGCGTCACCTGgcg                                    >  2:107194/1‑93 (MQ=255)

                                                     aTTGCGATGGACGGCAGCCAGAAGTTACCGCAGCGTCCGCTGGACCCGGTGCGTCTGCACCTGCAAAACGGCGGCAGCTGGCGTCACCTGgcg                                    >  2:219672/1‑93 (MQ=255)

                                                      ttGCGATGGACGGCAGCCAGAAGTTACCGCAGCGTCCGCTGGACCGGGTGCGTCTGCACCTGCAAAACGGCGGCAGCTGGCGTCACCTGgcgc                                   <  1:614420/93‑1 (MQ=255)

                                                      ttGCGATGGACGGCAGCCAGAAGTTACCGCAGCGTCCGCTGGACCCGGTGCGTCTGCACCTGCAAAACGGCGGCAGCTCGCGTCACCTGgcgc                                   <  1:4063872/93‑1 (MQ=255)

                                                      gtGCGATGGACGGCAGCCAGAAGTTACCGCAGCGTCCGCTGGACCCGGTGCGTCTGCACCTGCAAAACGGCGGCAGCTGGCGTCACCTGgcgc                                   <  2:1736981/92‑1 (MQ=255)

                                                         cGATGGACGGCAGCCAGAAGTTACCGCAGCGTCCGCTGGACCCGGTGCGTCTGCACCTGCAAAACGGCGGCAGCTGGCGTCACCTGGCGCTgg                                <  1:135925/93‑1 (MQ=255)

                                                          gATGGACGGCAGCCAGAAGTTACCGCAGCGTCCGCTGGACCCGGTGCGTCTGCACCTGCAAAACGGCGGCAGCTGGCGTCACCTCGCGCTggg                               >  1:4672026/1‑93 (MQ=255)

                                                           aTGGACGGCAGCCAGAAGTTACCGCAGCGTCCGCTGGACCCGGTGCGTCTGCACCTGCAAAACGGCGGCAGCTGGCGTCACCTGGCGCTGGGc                              <  2:1551403/93‑1 (MQ=255)

                                                            tGGACGGCAGCCAGAAGTTACCGCAGCGTCCGCTGGACCCGGTGCGTCTGCACCTGCAAAACGGCGGCAGCTGGCGTCACCTGGCGCTGGGCg                             >  2:3902909/1‑93 (MQ=255)

                                                             ggACGGCAGCCAGAAGTTACCGCAGCGTCCGCTGGACCCGGTGCGTCTGCACCTGCAAAACGGCGGCAGCTGGCGTCACCTGGCGCTGGGCGt                            >  1:6185316/1‑93 (MQ=255)

                                                              gACGGCAGCCAGAAGTTACCGCAGCGTCCGCTGGACCCGGTGCGTCTGCACCTGCAAAACGGCGGCAGCTGGCGTCACCTGGCGCTGGGCGtg                           <  2:1849867/93‑1 (MQ=255)

                                                              gACGGCAGCCAGAAGTTACCGCAGCGTCCGCTGGACCCGGTGCGTCTGCACCTGCAAAACGGCGGCAGCTGGCGTCACCTGGCGCTGGGCGtg                           <  1:3123047/93‑1 (MQ=255)

                                                                cGGCAGCCAGAAGTTACCGCAGCGTCCGCTGGACCCGGTGCGTCTGCACCTGCAAAACGGCGGCAGCTGGCGTCACCTGGCGCTGGGCGtggc                         <  1:1059067/93‑1 (MQ=255)

                                                                 ggCAGCCAGAAGTTACCGCAGCGTCCGCTGGACCCGGTGCGTCTGCACCTGCAAAACGGCGGCAGCTGGCGTCACCTGGCGCTGGGCGtggct                        <  1:4977670/93‑1 (MQ=255)

                                                                 ggCAGCCAGAAGTTACCGCAGCGTCCGCTGGACCCGGTGCGTCTGCACCTGCAAAACGGCGGCAGCTGGCGTCACCTGGCGCTGGGCGtggct                        <  2:818013/93‑1 (MQ=255)

                                                                 ggCAGCCAGAAGTTACCGCAGCGTCCGCTGGACCCGGTGCGTCTGCACCTGCAAAACGGCGGCAGCTGGCGTCACCTGGCGCTGGGCGtggct                        <  2:5704749/93‑1 (MQ=255)

                                                                    aGCCAGAAGTTACCGCAGCGTCCGCTGGACCCGGTGCGTCTGCACCTGCAAAACGGCGGCAGCTGGCGTCACCTGGCGCTGGGCGtggctcgc                     >  1:1841359/1‑93 (MQ=255)

                                                                     gCCAGAAGTTACCGCAGCGTCCGCTGGACCCGGTGCGTCTGCACCTGCAAAACGGCGGCAGCTGGCGTCACCTGGCGCTGGGCGTggctggct                    >  2:5259713/1‑93 (MQ=255)

                                                                     gCCAGAAGTTACCGCAGCGTCCGCTGGACCCGGTGCGTCTGCACCTGCAAAACGGCGGCAGCTGGCGTCACCTGGCGCTGGGCGTggctggct                    <  2:14687/93‑1 (MQ=255)

                                                                     gCCAGAAGTTACCGCAGCGTCCGCTGGACCCGGTGCGTCTGCACCTGCAAAACGGCGGCAGCTGGCGTCACCTGGCGCTGGGCGTggctggct                    >  2:3991550/1‑93 (MQ=255)

                                                                       cAGAAGTTACCGCAGCGTCCGCTGGACCCGGTGCGTCTGCACCTGCAAAACGGCGGCAGCTGGCGTCACCTGGCGCTGGGCGTGGctggctgg                  >  2:5475572/1‑93 (MQ=255)

                                                                        aGAAGTTACCGCAGCGTCCGCTGGACCCGGTGCGTCTGCACCTGCAAAACGGCGGCAGCTGGCGTCACCTGGCGCTGGGCGTGGCTGGCTGGa                 >  1:2012399/1‑93 (MQ=255)

                                                                        aGAAGTTACCGCAGCGTCCGCTGGACCCGGTGCGTCTGCACCTGCAAAACGGCGGCAGCTGGCGTCACCTGGCGCTGGGCGTGGCTGGCTGGa                 <  1:6311178/93‑1 (MQ=255)

                                                                         gAAGTTACCGCAGCGTCCGCTGGACCCGGTGCGTCTGCACCTGCAAAACGGCGGCAGCTGGCGTCACCTGGCGCTGGGCGTGGCTGGCTGGAt                <  2:2464099/93‑1 (MQ=255)

                                                                         gAAGTTACCGCAGCGTCCGCTGGACCCGGTGCGTCTGCACCTGCAAAACGGCGGCAGCTGGCGTCACCTGGCGCTGGGCGTGGCTGGCTGGAt                >  2:2039812/1‑93 (MQ=255)

                                                                         gAAGTTACCGCAGCGTCCGCTGGACCCGGTGCGTCTGCACCTGCAAAACGGCGGCAGCTGGCGTCACCTGGCGCTGGGCGTGGCTGGCTGGAt                <  2:3140229/93‑1 (MQ=255)

                                                                           agatACCGCAGCGTCCGCTGGACCCGGTGCGTCTGCACCTGCAAAACGGCGGCAGCTGGCGTCACCTGGCGCTGGGCGTGGCTGGCTGGATGc              >  1:4530617/4‑93 (MQ=255)

                                                                             ttACCGCAGCGTCCGCTGGACCCGGTGCGTCTGCACCTGCAAAACGGCGGCAGCTGGCGTCACCTGGCGCTGGGCGTGGCTGGCTGGATGGGt            >  1:36098/1‑93 (MQ=255)

                                                                             ttACCGCAGCGTCCGCTGGACCCGGTGCGTCTGCACCTGCAAAACGGCGGCAGCTGGCGTCACCTGGCGCTGGGCGTGGCTGGCTGGATGCGt            <  2:1299945/93‑1 (MQ=255)

                                                                             ttACCGCAGCGTCCGCTGGACCCGGTGCGTCTGCACCTGCAAAACGGCGGCAGCTGGCGTCACCTGGCGCTGGGCGTGGCTGGCTGGATGCGt            <  2:6419262/93‑1 (MQ=255)

                                                                             ttACCGCAGCGTCCGCTGGACCCGGTGCGTCTGCACCTGCAAAACGGCGGCAGCTGGCGTCACCTGGCGCTGGGCGTGGCTGGCTGGATGCGt            >  2:1612060/1‑93 (MQ=255)

                                                                               aCCGCAGCGTCCGCTGGACCCGGTGCGTCTGCACCTGCAAAACGGCGGCAGCTGGCGTCACCTGGCGCTGGGCGTGGCTGGCTGGATGCGTTa          >  2:6524757/1‑93 (MQ=255)

                                                                               aCCGCAGCGTCCGCTGGACCCGGTGCGTCTGCACCTGCAAAACGGCGGCAGCTGGCGTCACCTGGCGCTGGGCGTGGCTGGCTGGATGCGTTa          >  2:3635525/1‑93 (MQ=255)

                                                                                ccGCAGCGTCCGCTGGACCCGGTGCGTCTGCACCTGCAAAACGGCGGCAGCTGGCGTCACCTGGCGCTGGGCGTGGCTGGCTGGATGCGTTac         <  1:2373627/93‑1 (MQ=255)

                                                                                  gCAGCGTCCGCTGGACCCGGTGCGTCTGCACCTGCAAAACGGCGGCAGCTGGCGTCACCTGGCGCTGGGCGTGGCTGGCTGGGTGCGTTacac       >  1:4474531/1‑93 (MQ=255)

                                                                                   cAGCGTCCGCTGGACCCGGTGCGTCTGCACCTGCAAAACGGCGGCAGCTGGCGTCACCTGGCGCTGGGCGTGGCTGGCTGGATGCGTTTCAcc      >  1:2495759/1‑93 (MQ=255)

                                                                                     gCGTCCGCTGGACCCGGTGCGTCTGCACCTGCAAAACGGCGGCAGCTGGCGTCACCTGGCGCTGGGCGTGGCTGGCTGGATGCGTTACACCCa    >  2:16869/1‑93 (MQ=255)

                                                                                     gCGTCCGCTGGACCCGGTGCGTCTGCACCTGCAAAACGGCGGCAGCTGGCGTCACCTGGCGCTGGGCGTGGCTGGCTGGATGCGTTACAACCa    >  1:5740185/1‑93 (MQ=255)

                                                                                     gCGTCCGCTGGACCCGGTGCGTCTGCACCTGCAAAACGGCGGCAGCCGGCGTCACCTGGCGCTGGGCGTGGCTGGCTGGGTGCCGTACACCCa    >  1:3796816/1‑93 (MQ=255)

                                                                                      cGTCCGCTGGACCCGGTGCGTCTGCACCTGCAAAACGGCGGCAGCTGGCGTCACCTGGCGCTGGGCGTGGCTGGCTGGATGCGTTACACCCAg   <  1:2207583/93‑1 (MQ=255)

                                                                                      cGTCCGCTGGACCCGGTGCGTCTGCACCTGCAAAACGGCGGCAGCTGGCGTCACCTGGCGCTGGGCGTGGCTGGCTGGATGCGTTACACCCAg   <  1:1553953/93‑1 (MQ=255)

                                                                                      cGTCCGCTGGACCCGGTGCGTCTGCACCTGCAAAACGGCGGCAGCTGGCGTCACCTGGCGCTGGGCGTGGCTGGCTGGATGCGTTACACCCAg   <  1:5941597/93‑1 (MQ=255)

                                                                                       gTCCGCTGGACCCGGTGCGTCTGCACCTGCAAAACGGCGGCAGCTGGCGTCACCTGGCGCTGGGCGTGGCTGGCTGGATGCGTTACACCCAgg  >  1:4254074/1‑93 (MQ=255)

                                                                                       gTCCGCTGGACCCGGTGCGTCTGCACCTGCAAAACGGCGGCAGCTGGCGTCACCTGGCGCTGGGCGTGGCTGGCTGGATGCGTTACACCCAgg  <  2:6343134/93‑1 (MQ=255)

                                                                                       gTCCGCTGGACCCGGTGCGTCTGCACCTGCAAAACGGCGGCAGCTGGCGTCACCTGGCGCTGGGCGTGGCTGGCTGGATGCGTTACACCCAgg  >  2:3296857/1‑93 (MQ=255)

CGCTGCTGATCGAGCGTTTCAGCAACCCGTCTCTGCGTCACCGTACCTGGCAGATTGCGATGGACGGCAGCCAGAAGTTACCGCAGCGTCTGCTGGACCCGGTGCGTCTGCACCTGCAAAACGGCGGCAGCTGGCGTCACCTGGCGCTGGGCGTGGCTGGCTGGATGCGTTACACCCAGG  >  NC_000913/4551524‑4551703

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 20 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`