BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,603,785	A→G	noncoding (71/87 nt)	leuP ←	tRNA‑Leu

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,603,785	0	A	G	94.9%	638.3 / 26.2	215	noncoding (71/87 nt)	leuP	tRNA‑Leu
Reads supporting (aligned to +/- strand): ref base A (0/0); major base G (124/79); minor base . (6/5); total (130/85)
Fisher's exact test for biased strand distribution p-value = 7.55e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.41e-01

Only 100 of 239 total aligned reads displayed.

CACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGA‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAA  >  NC_000913/4603695‑4603874

cacCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGA‑GGGGGGGGGCt                                                                                         >  1:4054722/1‑93 (MQ=255)

 acCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCCTACTTTTTTTGATATCGCCTCGTTTGGTGCGA‑GGGGGGGGGCtt                                                                                        >  2:5948061/1‑93 (MQ=255)

   cTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGA‑GGGGGGGGCCTTGa                                                                                      >  2:1018540/1‑93 (MQ=255)

    tGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGA‑GGGGGGGGGCTTGaa                                                                                     <  2:2873516/93‑1 (MQ=255)

     gAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGA‑GGGGGGGGGCTTGAAc                                                                                    <  2:2640850/93‑1 (MQ=255)

       aGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGA‑GGGGGGGGGCTTGAAccc                                                                                  <  2:4958723/93‑1 (MQ=255)

       aGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGA‑GGGGGGGGGCTTGAAccc                                                                                  >  2:1901721/1‑93 (MQ=255)

       aGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGA‑GGGGGGGGGCTTGAAccc                                                                                  <  2:6483762/93‑1 (MQ=255)

       aGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGA‑GGGGGGGGGCTTGAAccc                                                                                  >  2:6034490/1‑93 (MQ=255)

        gCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGA‑GGGGGGGGGCTTGAAcccc                                                                                 >  2:5459928/1‑93 (MQ=255)

          tAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGA‑GGGGGGGGGCTTGAACCCCCa                                                                               <  2:1445283/93‑1 (MQ=255)

          tAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGA‑GGGGGGGGGCTTGAACCCCCa                                                                               <  2:2757403/93‑1 (MQ=255)

           aGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTCCTTTTTTTGATATCGCCTGGTTTGGTGCGA‑GGGGGGGGGCTTGAACCCCCAc                                                                              <  1:6425688/93‑1 (MQ=255)

           aGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGA‑GGGGGGGGGCTTGAACCCCCAc                                                                              <  1:3228694/93‑1 (MQ=255)

           aGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGA‑GGGGGGGGGCTTGAACCCCCAc                                                                              >  2:1526642/1‑93 (MQ=255)

            gcgcGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGA‑GGGGGGGGGCTTGAACCCCCACg                                                                             >  2:3254036/1‑93 (MQ=255)

             cgcgTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGA‑GGGGGGGGGCTTGAACCCCCACGt                                                                            <  2:3741958/93‑1 (MQ=255)

             cgcgTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGA‑GGGGGGGGGCTTGAACCCCCACGt                                                                            <  1:3977403/93‑1 (MQ=255)

              gcgTCTACCAATTCCGCCCCCTTCGCAAAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGA‑GGGGGGGGGCTTGAACCCCCACGTc                                                                           <  2:1402354/93‑1 (MQ=38)

              gcgTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGA‑GGGGGGGGGCTTGAACCCCCACGTc                                                                           <  2:3240953/93‑1 (MQ=255)

              gcgTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGA‑GGGGGGGGGCTTGAACCCCCACGTc                                                                           >  2:85266/1‑93 (MQ=255)

               cgTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGA‑GGGGGGGGGCTTGAACCCCCACGTcc                                                                          >  2:1269209/1‑93 (MQ=255)

                gTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGA‑GGGGGGGGGCTTGAACCCCCACGTCCg                                                                         >  1:2560272/1‑93 (MQ=255)

                 tCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGA‑GGGGGGGGGGTTGGATCCCCCCGttcat                                                                        >  1:1414881/1‑89 (MQ=38)

                 tCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGA‑GGGGGGGGGCTTGCAGCCCCACGTCCGt                                                                        >  1:5166812/1‑93 (MQ=255)

                 tCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGA‑GGGGGGGGGCTTGAACCCCCACGTCCGt                                                                        >  1:6519529/1‑93 (MQ=255)

                 tCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGA‑GGGGGGGGGCTTGAACCCCCACGTCCGt                                                                        >  2:2016182/1‑93 (MQ=255)

                   tACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTC‑ATTGGGGCGA‑GGGGGGGGGCTTGTATCCCCACCTCCggtag                                                                     >  1:4683441/1‑89 (MQ=255)

                     ccAATTCCGCCACCTTCGCCCAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGA‑GGGGGGGGGCTTGAACCCCCACGTCCGTAAGa                                                                    <  1:6269076/93‑1 (MQ=255)

                      cAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGA‑GGGGGGGGGCTTGAACCCCCACGTCCGTAAGaa                                                                   >  1:4247917/1‑93 (MQ=255)

                      cAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGCGCGA‑GGGGGGGGGCTTGAACCCCCCGGTCCGTAAgca                                                                   >  2:2940078/1‑91 (MQ=38)

                        aTTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGA‑GGGGGGGGGCTTGAACCCCCACGTCCGTAAGAaca                                                                 >  1:3028702/1‑93 (MQ=255)

                        aTTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGA‑GGGGGGGGGCTTGAACCCCCACGTCCGTAAGAaca                                                                 >  1:5681642/1‑93 (MQ=255)

                        aTTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGA‑GGGGGGGGGCTTGAACCCCCACCTCCGTAAGAacc                                                                 >  1:1527919/1‑92 (MQ=255)

                        aTTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGA‑GGGGGGGG‑CTTGAACCCCCACGTCCGTAAGAacac                                                                >  2:4350959/1‑93 (MQ=255)

                             gCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGA‑GGGGGGGGGCTTGAACCCCCACGTCCGTAAGAACACTAac                                                            >  1:6679060/1‑93 (MQ=255)

                               cACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGA‑GGGGGGGGGCTTGAACCCCCACGTCCGTAAGAACACTAacac                                                          >  2:1978275/1‑93 (MQ=255)

                               cACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGA‑GGGGGGGG‑CTTGAACCCCCACGTCCGTAAGCACACTAACAcc                                                         >  1:393350/1‑93 (MQ=255)

                                aCCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAGGGGGGGGGGCTTGAACCCCCACGTCCGTAAGAACACTAacac                                                          >  1:850458/1‑93 (MQ=255)

                                aCCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGA‑GGGGGGGGGCTTGAACCCCCACGTCCGTAAGAACACTAACAcc                                                         >  1:1965002/1‑93 (MQ=255)

                                aCCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGA‑GGGGGGGGGCTTGAACCCCCACGTCCGTAAGAACACTAACAcc                                                         >  2:1724614/1‑93 (MQ=255)

                                aCCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGA‑GGGGGGGGGCTTGAACCCCCACGTCCGTAAGAACACTAACAcc                                                         >  2:2177239/1‑93 (MQ=255)

                                 ccTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGA‑GGGGGGGGGCTTGAACCCCCACGTCCGTAAGAACACTAACACCt                                                        >  2:4197072/1‑93 (MQ=255)

                                  cTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGA‑GGGGGGGGGCTTGAACCCCCACGTCCGTAAGAACACTAACACCTg                                                       <  2:4103055/93‑1 (MQ=255)

                                   ttCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGA‑GGGGGGGGGCTTGAACCCCCACGTCCGTAAGAACACTAACACCTGa                                                      >  1:1818334/1‑93 (MQ=255)

                                   ttCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGA‑GGGGGGGGGCTTGAACCCCCACGTCCGTAAGAACACTAACACCTGa                                                      >  2:1634827/1‑93 (MQ=255)

                                     cGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGA‑GGGGGGGGGCTTGAACCCCCACGTCCGTAAGAAAACTAAAACCTGaac                                                    >  1:4843341/1‑92 (MQ=25)

                                      ccacaGTCATCTACCTTTTTTTGATATCCCCTCGTTTGGTGCGA‑GGGGGGGGGCTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGc                                                   <  1:5458581/92‑1 (MQ=18)

                                        acaTTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGA‑GGGGGGGGGCTTGAACCCACACGTCCGTAAGAACACTAACACCTGAAGCTa                                                 <  1:4127431/93‑1 (MQ=255)

                                         caGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGA‑GGGGGGGGGCTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAg                                                >  2:5424040/1‑93 (MQ=255)

                                          aGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGA‑GGGGGGGGGCTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAgc                                               <  2:4991930/93‑1 (MQ=255)

                                          aGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGA‑GGGGGGGGGCTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAgc                                               <  2:2521553/93‑1 (MQ=255)

                                           gTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGA‑GGGGGGGGGCTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAgcg                                              <  2:5737095/93‑1 (MQ=255)

                                              aTCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGA‑GGGGGGGGGCTTGAACCCCCACGTCCGTAAGAACACTAACACCTCAAGCTAGCGCGt                                           <  2:3589592/93‑1 (MQ=255)

                                               tCTTATTTTTTTTGATATCGCCTCGTTTGGTGCGA‑GGGGGGGGGCTTGACCCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTc                                          <  2:4807239/93‑1 (MQ=255)

                                               tCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGA‑GGGGGGGGGCTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTc                                          <  1:2284182/93‑1 (MQ=255)

                                               tCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGA‑GGGGGGGGGCTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTc                                          <  1:6224699/93‑1 (MQ=255)

                                               tCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGA‑GGGGGGGGGCTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTc                                          <  1:1099437/93‑1 (MQ=255)

                                                cTTACTTTTTTTGATATCGCCTCGTTTGGTGCGA‑GGGGGGGGGCTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCt                                         >  2:6277954/1‑93 (MQ=255)

                                                 ttACTTTTTTTGATATCGCCTCGTTTGGTGCGA‑GGGGGGGGGCTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTa                                        >  2:133967/1‑93 (MQ=255)

                                                 ttACTTTTTTTGATATCGCCTCGTTTGGTGCGA‑GGGGGGGGGCTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTa                                        >  2:1783870/1‑93 (MQ=255)

                                                 ttACTTTTTTTGATATCGCCTCGTTTGGTGCGA‑GGGGGGGG‑CTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTAc                                       >  1:1057949/1‑93 (MQ=255)

                                                   aCTTTTTTTGATATCGCCTCGTTTGGTGCGA‑GGGGGGGGGCTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTAcc                                      <  2:3061572/93‑1 (MQ=255)

                                                   aCTTTTTTTGATATCGCCTCGTTTGGTGCGA‑GGGGGGGGGCTTGAACCCCCACGTCCGTAAGAAAACTAACACCTGAAGCTAGCGCGTCTAcc                                      >  2:3009482/1‑93 (MQ=255)

                                                     tttttttGATATCGCCTCGTTTGGTGCGA‑GGGGGGGGGGTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCaa                                    >  1:3419537/1‑93 (MQ=255)

                                                     tttttttGATATCGCCTCGTTTGGTGCGA‑GGGGGGGGGCTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCaa                                    >  1:5126833/1‑93 (MQ=255)

                                                     tttttttGATATCGCCTCGTTTGGTGCGA‑GGGGGGGGGCTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCaa                                    >  1:533626/1‑93 (MQ=255)

                                                      ttttttGATATCGCCTCGTTTGGTGCGA‑GGGGGGGGGCTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAAt                                   >  2:3184672/1‑93 (MQ=255)

                                                        ttttGATATCGCCTCGTTTGGTGCGA‑GGGGGGGGGCTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTc                                 <  1:2106570/93‑1 (MQ=255)

                                                         tttGATATCGCCTCGTTTGGTGCGA‑GGGGGGGGGCTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTcc                                >  2:4689425/1‑93 (MQ=255)

                                                           tGATATCGCCTCGTTTGGTGCGA‑GGGGGGGGGCTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGc                              >  1:6454290/1‑93 (MQ=255)

                                                            gATATCGCCTCGTTTGGTGCGA‑GGGGGGGGGCTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGcc                             >  1:2650783/1‑93 (MQ=255)

                                                            gATATCGCCTCGTTTGGTGCGA‑GGGGGGGGGCTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGcc                             >  1:4519481/1‑93 (MQ=255)

                                                             atatCGCCTCGTTTGGTGCGA‑GGGGGGGGGCTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGccc                            >  1:735027/1‑92 (MQ=255)

                                                             atatCGCCTCGTTTGGTGCGA‑GGGGGGGGGCTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCa                            >  2:2982625/1‑93 (MQ=255)

                                                              tatCGCCTCGTTTGGTGCGA‑GGGGGGGGGCTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCAc                           >  2:5363530/1‑93 (MQ=255)

                                                              tatCGCCTCGTTTGGTGCGA‑GGGGGGGGGCTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCAc                           >  1:3523123/1‑93 (MQ=255)

                                                              tatCGCCTCGTTTGGTGCGA‑GGGGGGGGGCTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCCCCAc                           >  1:5555345/1‑93 (MQ=255)

                                                              tatCGCCTCGTTGGGTGCGA‑GGGGGGGG‑CTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCAcc                          <  2:2049020/93‑1 (MQ=255)

                                                               atCGCCTCGTTTGGTGCGA‑GGGGGGGG‑CTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCt                         <  2:5495822/93‑1 (MQ=255)

                                                                tCGCCTCGTTTGGTGCGA‑GGGGGGGGGCTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCt                         <  1:5231273/93‑1 (MQ=255)

                                                                tCGCCTCGTTTGGTGCGA‑GGGGGGGGGCTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCt                         >  1:1196626/1‑93 (MQ=255)

                                                                tCGCCTCGTTTGGTGCGA‑GGGGGGGGGCTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCt                         <  1:1009681/93‑1 (MQ=255)

                                                                 cGCCTCGTTTGGTGCGA‑GGGGGGGGGCTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCtt                        >  2:3365513/1‑93 (MQ=255)

                                                                 cGCCTCGTTTGGTGCGA‑GGGGGGGG‑CTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTc                       <  1:2015579/93‑1 (MQ=255)

                                                                   ccTCGTTTGGTGCGA‑GGGGGGGGGCTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCg                      >  1:2813945/1‑93 (MQ=255)

                                                                     tCGTTTGGTGCGA‑GGGGGGGGGCTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGcc                    >  2:6556014/1‑93 (MQ=255)

                                                                      cGTTTGGTGCGA‑GGGGGGGGGCTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGccc                   >  1:4371502/1‑93 (MQ=255)

                                                                        tttGGTGCGA‑GGGGGGGGGCTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAg                 >  1:572264/1‑93 (MQ=255)

                                                                         ttGGTGCGA‑GGGGGGGGCCTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGt                <  1:6036180/93‑1 (MQ=37)

                                                                           ggTGCGA‑GGGGGGGGGCTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGc              <  2:6419400/93‑1 (MQ=255)

                                                                               cGA‑GGGGGGGGGCTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGc          <  2:3341905/93‑1 (MQ=255)

                                                                                   ggggggggGCTTGACCCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAat       <  1:1286511/93‑1 (MQ=255)

                                                                                   ggggggggGCTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAat       >  2:1689909/1‑93 (MQ=255)

                                                                                   ggggggggGCTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAat       <  1:1716528/93‑1 (MQ=255)

                                                                                     ggggggGCTTGACCCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAatat     <  1:1259299/93‑1 (MQ=255)

                                                                                      ggggggCTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATc    >  2:3076531/1‑93 (MQ=255)

                                                                                      ggggggCTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATc    >  2:345204/1‑93 (MQ=255)

                                                                                       ggggGCTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCa   >  2:3998294/1‑93 (MQ=255)

                                                                                        ggggCTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCaa  <  1:6250655/93‑1 (MQ=255)

CACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGA‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAA  >  NC_000913/4603695‑4603874

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 18 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`