BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,604,832	A→G	R146R (CGT→CGC)	yjjT ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,604,832	0	A	G	99.6%	900.3 / NA	278	R146R (CGT→CGC)	yjjT	hypothetical protein
Reads supporting (aligned to +/- strand): ref base A (0/0); major base G (138/139); minor base T (0/1); total (138/140)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 293 total aligned reads displayed.

AAACACGCCAGGCAGCGTTTTGACCGTCAGGCCATCGACGCTGTATTCGCCCCAGAATTTCTCCGCATCAAATACCGGCTGTTTTTCCAGACGACCAAAATAGAGGCCACAGCGACGAGCGCTGTCGACTTTATTCAACGGCGCATAATCTGCCAGCATCTGCTCGGCGCTGCGCACGCC  >  NC_000913/4604742‑4604921

  acacGCCAGGCAGCGTTTTGACCGTCAGGCCATCGACGCTGTATTCGCCCCAGAATTTCTCCGCATCAAATACCGGCTGTTTTTCCAggcgac                                                                                       <  1:660499/93‑1 (MQ=255)

   cacGCCAGGCAGCGTTTTGACCGTCAGGCCATCGACGCTGTATTCGCCCCAGAATTTCTCCGCATCAAATACCGGCTGTTTTTCCAGGCGAcc                                                                                      <  2:1520566/93‑1 (MQ=255)

     cGCCAGGCAGCGTTTTGACCGTCAGGCCATCGACGCTGTATTCGCCCCAGAATTTCTCCGCATCAAATACCGGCTGTTTTTCCAGGCGACCat                                                                                    <  1:6435309/93‑2 (MQ=255)

        cAGGCAGCGTTTTGACCGTCAGGCCATCGACGCTGTATTCGCCCCAGAATTTCTCCGCATCAAATACCGGCTGTTTTTCCAGGCGACCAAAAt                                                                                 <  1:4787974/93‑1 (MQ=255)

         cggCAGCGTTTTGACCGTCAGGCCATCGACGCTGTATTCGCCCCAGAATTTCTCCGCATCAAATACCGGCTGTTTTTCCAGGCGACCAAAATa                                                                                <  2:5531760/92‑1 (MQ=255)

         aGGCAGCGTTTTGACCGTCAGGCCATCGACGCTGTATTCGCCCCAGAATTTCTCCGCATCAAATACCGGCTGTTTTTCCAGGCGACCAAAATa                                                                                <  1:1487647/93‑1 (MQ=255)

         aGGCAGCGTTTTGACCGTCAGGCCATCGACGCTGTATTCGCCCCAGAATTTCTCCGCATCAAATACCGGCTGTTTTTCCAGGCGACCAAAATa                                                                                <  2:6464625/93‑1 (MQ=255)

           gCAGCGTTTTGACCGTCAGGCCATCGACGCTGTATTCGCCCCAGAATTTCTCCGCATCAAATACCGGCTGTTTTTCCAGGCGACCAAAATaga                                                                              <  1:570046/93‑1 (MQ=255)

           gCAGCGTTTTGACCGTCAGGCCATCGACGCTGTATTCGCCCCAGAATTTCTCCGCATCAAATACCGGCTGTTTTTCCAGGCGACCAAAATaga                                                                              <  2:3602532/93‑1 (MQ=255)

            cAGCGTTTTGACCGTCAGGCCATCGACGCTGTATTCGCCCCAGAATTTCTCCGCATCAAATACCGGCTGTTTTTCCAGGCGACCAAAATagag                                                                             <  1:6059734/93‑1 (MQ=255)

              gCGTTTTGACCGTCAGGCCATCGACGCTGTATTCGCCCCAGAATTTCTCCGCATCAAATACCGGCTGTTTTTCCAGGCGACCAAAATAGAGGc                                                                           >  1:946090/1‑93 (MQ=255)

              gCGTTTTGACCGTCAGGCCATCGACGCTGTATTCGCCCCAGAATTTCTCCGCATCAAATACCGGCTGTTTTTCCAGGCGACCAAAATAGAGGc                                                                           >  2:2713334/1‑93 (MQ=255)

               cGTTTTGACCGTCAGGCCATCGACGCTGTATTCGCCCCAGAATTTCTCCGCATCAAATACCGGCTGTTTTTCCAGGCGACCAAAATAGAGGcc                                                                          <  1:6678572/93‑1 (MQ=255)

                gTTTTGACCGTCAGGCCATCGACGCTGTATTCGCCCCAGAATTTCTCCGCATCAAATACCGGCTGTTTTTCCAGGCGACCAAAATAGAGGCca                                                                         <  1:5348832/93‑1 (MQ=255)

                gTTTTGACCGTCAGGCCATCGACGCTGTATTCGCCCCAGAATTTCTCCGCATCAAATACCGGCTGTTTTTCCAGGCGACCAAAATAGAGGCca                                                                         >  1:1270662/1‑93 (MQ=255)

                 ttttGACCGTCAGGCCATCGACGCTGTATTCGCCCCAGAATTTCTCCGCATCAAGTACCGGCTGTTTTTCCAGGCGACCAAAATAGAGGCcac                                                                        >  2:3778452/1‑93 (MQ=255)

                 ttttGACCGTCAGGCCATCGACGCTGTATTCGCCCCAGAATTTCTCCGCATCAAATACCGGCTGTTTTTCCAGGCGACCAAAATAGAGGCcac                                                                        <  2:5038183/93‑1 (MQ=255)

                 ttttGACCGTCAGGCCATCGACGCTGTATTCGCCCCAGAATTTCTCCGCATCAAATACCGGCTGTTTTTCCAGGCGACCAAAATAGAGGCcac                                                                        <  1:4058828/93‑1 (MQ=255)

                 ttttGACCGTCAGGCCATCGACGCTGTATTCGCCCCAGAATTTCTCCGCATCAAATACCGGCTGTTTTTCCAGGCGACCAAAATAGAGGCcac                                                                        >  1:3957613/1‑93 (MQ=255)

                 ttttGACCGTCAGGCCATCGACGCTGTATTCGCCCCAGAATTTCTCCGCATCAAATACCGGCTGTTTTTCCAGGCGACCAAAATAGAGGCcac                                                                        >  1:2105943/1‑93 (MQ=255)

                 ttttGACCGTCAGGCCATCGACGCTGTATTCGCCCCAGAATTTCTCCGCATCAAATACCGGCTGTTTTTCCAGGCGACCAAAATAGAGGCcac                                                                        >  1:1406403/1‑93 (MQ=255)

                  tttGACCGTCAGGCCATCGACGCTGTATTCGCCCCAGAATTTCTCCGCACCAAATACCGGCTGTTTTTCCAGGCGACCAAAATAGAGGCcaca                                                                       <  1:6092281/93‑1 (MQ=255)

                    tGACCGTCAGGCCATCGACGCTGTATTCGCCCCAGAATTTCTCCGCATCAAATACCGGCTGTTTTTCCAGGCGACCAAAATAGAGGCCACAGc                                                                     >  1:4172012/1‑93 (MQ=255)

                    tGACCGTCAGGCCATCGACGCCGTTTTTGACCCAGAATTTCTCCGCATCAAATACCGGCTGCTGTTCCAGGCGACCAAAATAGAGGCCAAAGc                                                                     >  1:5630599/1‑93 (MQ=255)

                      aCCGTCAGGCCATCGACGCTGTATTCGCCCCAGAATTTCTCCGCATCAAATACCGGCTGTTTTTCCAGGCGACCAAAATAGAGGCCACAGcga                                                                   <  1:3405862/93‑1 (MQ=255)

                      aCCGTCAGGCCATCGACGCTGTATTCGCCCCAGAATTTCTCCGCATCAAATACCGGCTGTTTTTCCAGGCGACCAAAATAGAGGCCACAGcga                                                                   <  1:3133284/93‑1 (MQ=255)

                         gTCAGGCCATCGACGCTGTATTCGCCCCAGAATTTCTCCGCATCAAATACCGGCTGTTTTTCCAGGCGACCAAAATAGAGGCCACAGcgacga                                                                <  2:3207152/93‑1 (MQ=255)

                         gTCAGGCCATCGACGCTGTATTCGCCCCAGAATTTCTCCGCATCAAATACCGGCTGTTTTTCCAGGCGACCAAAATAGAGGCCACAGcgacga                                                                <  2:1814803/93‑1 (MQ=255)

                          tCAGGCCATCGACGCTGTATTCGCCCCAGAATTTCTCCGCATCAAATACCGGCTGTTTTTTCAGGCGACCAAAATAGAGGCCAAAGCGACGAg                                                               >  1:2899986/1‑93 (MQ=255)

                           cAGGCCATCCACGCTGTATTCGCCCCAGAATTTCTCCGCATCAAATACCGGCTGTTTTTCCAGGCGCCCAAAATAGAGGCCCCAGCGACGAgc                                                              <  2:4216433/93‑1 (MQ=255)

                             ggCCATCGACGCTGTATTCGCCCCAGAATTTCTCCGCATCAAATACCGGCTGTTTTTCCAGGCGACCAAAATAGAGGCCACAGCGACGAgcgc                                                            <  1:2776075/93‑1 (MQ=255)

                                 aTCGACGCTGTATTCGCCCCAGAATTTCTCCGCATCAAATACCGGCTGTTTTTCCAGGCGACCAAAATAGAGGCCACAGCGACGAGCGCTGTc                                                        <  1:3021688/93‑1 (MQ=255)

                                 aTCGACGCTGTATTCGCCCCAGAATTTCTCCGCATCAAATACCGGCTGTTTTTCCAGGCGACCAAAATAGAGGCCACAGCGACGAGCGCTGTc                                                        >  2:2053696/1‑93 (MQ=255)

                                 aTCGACGCTGTATTCGCCCCAGAATTTCTCCGCATCAAATACCGGCTGTTTTTCCAGGCGACCAAAATAGAGGCCACAGCGACGAGCGCTGTc                                                        <  2:6516588/93‑1 (MQ=255)

                                  tCGACGCTGTATTCGCCCCAGAATTTCTCCGCATCAAATACCGGCTGTTTTTCCAGGCGACCAAAATAGAGGCCACAGCGACGAGCGCTGTCg                                                       <  2:4286744/93‑1 (MQ=255)

                                  tCGACGCTGTATTCGCCCCAGAATTTCTCCGCATCAAATACCGGCTGTTTTTCCAGGCGACCAAAATAGAGGCCACAGCGACGAGCGCTGTCg                                                       >  1:2568429/1‑93 (MQ=255)

                                   cGACGCTGTATTCGCCCCAGAATTTCTCCGCATCAAATACCGGCTGTTTTTCTAGGCGACCGAAATAGAGGTCACAGCGACGAGCGCTGCCgc                                                      >  1:2055567/1‑92 (MQ=255)

                                   cGACGCTGTATTCGCCCCAGAATTTCTCCGCATCAAATACCGGCTGTTTTTCCAGGCGACCAAAATAGAGGCCACAGCGACGAGCGCTGTCGa                                                      <  2:890613/93‑1 (MQ=255)

                                    gACGCTGTATTCGCCCCAGAATTTCTCCGCATCAAATACCGGCTGTTTTTCCAGGCGACCAAAATAGAGGCCACAGCGACGAGCGCTGTCGAc                                                     <  2:1980891/93‑1 (MQ=255)

                                    gACGCTGTATTCGCCCCAGAATTTCTCCGCATCAAATACCGGCTGTTTTTCCAGGCGACCAAAATAGAGGCCACAGCGACGAGCGCTGTCGAc                                                     <  2:307454/93‑1 (MQ=255)

                                     aCGCTGTATTCGCCCCAGAATTTCTCCGCATCAAATACCGGCTGTTTTTCCAGGCGACCAAAATAGAGGCCACAGCGACGAGCGCTGTCGACt                                                    <  2:3229131/93‑1 (MQ=255)

                                     aCGCTGTATTCGCCCCAGAATTTCTCCGCATCAAATACCGGCTGTTTTTCCAGGCGACCAAAATAGAGGCCACAGCGACGAGCGCTGTCGACt                                                    >  2:5418190/1‑93 (MQ=255)

                                       gCTGTATTCGCCCCAGAATTTCTCCGCATCAAATACCGGCTGTTTTTCCAGGCGACCAAAATAGAGGCCACAGCGACGAGCGCTGTCGACttt                                                  >  2:5150858/1‑93 (MQ=255)

                                       gCTGTATTCGCCCCAGAATTTCTCCGCATCAAATACCGGCTGTTTTTCCAGGCGACCAAAATAGAGGCCACAGCGACGAGCGCTGTCGACttt                                                  >  2:2359326/1‑93 (MQ=255)

                                        cTGTATTCTCCCAAGAATTTCTCCGCATCAAATACCGGCTGTTTTTCCAGGCGACCAAAATAGAGGCCACAGCGACGAGCGCTGTCGACTTTa                                                 <  1:166969/93‑1 (MQ=255)

                                        cTGTATTCGCCCCAGAATTTCTCCGCATCAAATACCGGCTGTTTTTCCAGGCGACCAAAATAGAGGCCACAGCGACGAGCGCTGTCGACTTTa                                                 <  1:5542202/93‑1 (MQ=255)

                                        cTGTATTCGCCCCAGAATTTCTCCGCATCAAATACCGGCTGTTTTTCCAGGCGACCAAAATAGAGGCCACAGCGACGAGCGCTGTCGACTTTa                                                 >  2:5779137/1‑93 (MQ=255)

                                         tGTATTCGCCCCAGAATTTCTCCGCATCAAATACCGGCTGTTTTTCCAGGCGACCAAAATAGAGGCCACAGCGACGAGCGCTGTCGACTTTAt                                                >  2:3372018/1‑93 (MQ=255)

                                          gTATTCGCCCCAGAATTTCTCCGCATCAAATACCGGCTGTTTTTCCAGGCGACCAAAATAGAGGCCACAGCGACGAGCGCTGTCGACTTTAtt                                               >  2:1666250/1‑93 (MQ=255)

                                           tATTCGCCCCAGAATTTCTCCGCATCAAATACCGGCTGTTTTTCCAGGCGACCAAAATAGAGGCCACAGCGACGAGCGCTGTCGACTTTATTc                                              >  1:4581670/1‑93 (MQ=255)

                                            aTTCGCCCCAGAATTTCTCCGCATCAAATACCGGCTGTTTTTCCAGGCGACCAAAATAGAGGCCACAGCGACGAGCGCTGTCGACTTTATTCa                                             <  1:1010815/93‑1 (MQ=255)

                                             ttCGCCCCAGAATTTCTCCGCATCAAATACCGGCTGTTTTTCCAGGCGACCAAAATAGAGGCCACAGCGACGAGCGCTGTCGACTTTATTCaa                                            <  2:2894697/93‑1 (MQ=255)

                                                gCCCCAGAATTTCTCCGCATCAAATACCGGCTGTTTTTCCAGGCGACCAAAATAGAGGCCACAGCGACGAGCGCTGTCGACTTTATTCAACgg                                         >  1:4424978/1‑93 (MQ=255)

                                                gCCCCAGAATTTCTCCGCATCAAATACCGGCTGTTTTTCCAGGCGACCAAAATAGAGGCCACAGCGACGAGCGCTGTCGACTTTATTCAACgg                                         >  2:1573352/1‑93 (MQ=255)

                                                 ccccAGAATTTCTCCGCATCAAATACCGGCTGTTTTTCCAGGCGACCAAAATAGAGGCCACAGCGACGAGCGCTGTCGACTTTATTCAACGgc                                        <  2:4449687/93‑1 (MQ=255)

                                                   ccAGAATTTCTCCGCATCAAATACCGGCTGTTTTTCCAGGCGACCAAAATAGAGGCCACAGCGACGAGCGCTGTCGACTTTATTCAACGgcgc                                      >  2:6681973/1‑93 (MQ=255)

                                                    cAGAATTTCTCCGCATCAAATACCGGCTGTTTTTCCAGGCGACCAAAATAGAGGCCACAGCGACGAGCGCTGTCGACTTTATTCAACGGCGCa                                     >  2:3701793/1‑93 (MQ=255)

                                                      gAATTTCTCCGCATCAAATACCGGCTGTTTTTCCAGGCGACCAAAATAGAGGCCACAGCGACGAGCGCTGTCGACTTTATTCAACGGCGCATa                                   >  2:1195671/1‑93 (MQ=255)

                                                      gAATTTCTCCGCATCAAATACCGGCTGTTTTTCCAGGCGACCAAAATAGAGGCCACAGCGACGAGCGCTGTCGACTTTATTCAACGGCGCATa                                   <  1:5287695/93‑1 (MQ=255)

                                                        aTTTCTCCGCATCAAATACCGGCTGTTTTTCCAGGCGACCAAAATAGAGGCCACAGCGACGAGCGCTGTCGACTTTATTCAACGGCGCATAAt                                 >  1:4477978/1‑93 (MQ=255)

                                                        aTTTCTCCGCATCAAATACCGGCTGTTTTTCCAGGCGACCAAAATAGAGGCCACAGCGACGAGCGCTGTCGACTTTATTCAACGGCGCATAAt                                 >  2:3518003/1‑93 (MQ=255)

                                                         tttCTCCGCATCAAATACCGGCTGTTTTTCCAGGCGACCAAAATAGAGGCCACAGCGACGAGCGCTGTCGACTTTATTCAACGGCGCATAATc                                <  1:1771773/93‑1 (MQ=255)

                                                         tttCTCCGCATCAAATACCGGCTGTTTTTCCAGGCGACCAAAATAGAGGCCACAGCGACGAGCGCTGTCGACTTTATTCAACGGCGCATAATc                                <  2:1960676/93‑1 (MQ=255)

                                                         tttCTCCGCATCAAATACCGGCTGTTTTTCCAGGCGACCAAAATAGAGGCCACAGCGACGAGCGCTGTCGACTTTATTCAACGGCGCATAATc                                <  1:4911775/93‑1 (MQ=255)

                                                         tttCTCCGCATCAAATACCGGCTGTTTTTCCAGGCGACCAAAATAGAGGCCACAGCGACGAGCGCTGTCGACTTTATTCAACGGCGCATAATc                                >  2:4569747/1‑93 (MQ=255)

                                                         tttCTCCGCATCAAATACCGGCTGTTTTTCCAGGCGACCAAAATAGAGGCCACAGCGACGAGCGCTGTCGACTTTATTCAACGGCGCATAATc                                <  2:3295599/93‑1 (MQ=255)

                                                         tttCTCCGCATCAAATACCGGCTGTTTTTCCAGGCGACCAAAATAGAGGCCACAGCGACGAGCGCTGTCGACTTTATTCAACGGCGCATAATc                                >  1:5767807/1‑93 (MQ=255)

                                                         tttCTCCGCATCAAATACCGGCTGTATTTCCAGGCGACCAAAATAGAGGCCACAGCGACGAGCGCTGACGACTTTATTCAACGGCGCATAATc                                >  1:545889/1‑93 (MQ=255)

                                                           tctcCGCATCAAATACCGGCTGTTTTTCCAGGCGACCAAAATAGAGGCCACAGCGACGAGCGCTGTCGACTTTATTCAACGGCGCATAATCTg                              <  2:5444439/93‑1 (MQ=255)

                                                            ctcCGCATCAAATACCGGCTGTTTTTCCAGGCGACCAAAATAGAGGCCACAGCGACGAGCGCTGTCGACTTTATTCAACGGCGCATAATCTGc                             >  1:3271367/1‑93 (MQ=255)

                                                             tcCGCATCAAATACCGGCTGTTTTTCCAGGCGACCAAAATAGAGGCCACAGCGACGAGCGCTGTCGACTTTATTCAACGGCGCATAATCTGcc                            >  1:2255721/1‑93 (MQ=255)

                                                               cGCATCAAATACCGGCTGTTTTTCCAGGCGACCAAAATAGAGGCCACAGCGACGAGCGCTGTCGACTTTATTCAACGGCGCATAATCTGCCAg                          <  2:5828861/93‑1 (MQ=255)

                                                                gCATCAAATACCGGCTGTTTTTCCAGGCGACCAAAATAGAGGCCACAGCGACGAGCGCTGTCGACTTTATTCAACGGCGCATAATCTGCCAGc                         >  1:2468741/1‑93 (MQ=255)

                                                                 cATCAAATACCGGCTGTTTTTCCAGGCGACCAAAATAGAGGCCACAGCGACGAGCGCTGTCGACTTTATTCAACGGCGCATAATCTGCCAGCa                        <  2:6263155/93‑1 (MQ=255)

                                                                  aTCAAATACCGGCTGTTTTTCCAGGCGACCAAAATAGAGGCCACAGCGACGAGCGCTGTCGACTTTATTCAACGGCGCATAATCTGCCAGCAt                       <  1:3791644/93‑1 (MQ=255)

                                                                  aTCAAATACCGGCTGTTTTTCCAGGCGACCAAAATAGAGGCCACAGCGACGAGCGCTGTCGACTTTATTCAACGGCGCATAATCTGCCAGCAt                       <  1:1809900/93‑1 (MQ=255)

                                                                  aTCAAATACCGGCTGTTTTTCCAGGCGACCAAAATAGAGGCCACAGCGACGAGCGCTGTCGACTTTATTCAACGGCGCATAATCTGCCAGCAt                       <  1:1070366/93‑1 (MQ=255)

                                                                  aTCAAATACCGGCTGTTTTTCCAGGCGACCAAAATAGAGGCCACAGCGACGAGCGCTGTCGACTTTATTCAACGGCGCATAATCTGCCAGCAt                       >  2:4059235/1‑93 (MQ=255)

                                                                  aTCAAATACCGGCTGTTTTTCCAGGCGACAAAAATAGAGGCCACAGCGACGAGCGCTGTCGACTTTATTCAACGGCGCATAATCTGCCAGCAt                       <  1:3500201/93‑1 (MQ=255)

                                                                   tCAAATACCGGCTGTTTTTCCAGGCGACCAAAATAGAGGCCACAGCGACGAGCGCTGTCGACTTTATTTCACGGCGCATAATCTGCTAGCATc                      >  1:5149816/1‑93 (MQ=255)

                                                                   tCAAATACCGGCTGTTTTTCCAGGCGACCAAAATAGAGGCCACAGCGACGAGCGCTGTCGACTTTATTCAACGGCGCATAATCTGCCAGCATc                      <  2:1078508/93‑1 (MQ=255)

                                                                    cAAATACCGGCTGTTTTTCCAGTCGACCAAAATAGAGGCCACAGCGACGAGCGCTGTCGACTTTATTCAACGGCGCATAATCTGCCAGCATCt                     <  2:5373372/93‑1 (MQ=255)

                                                                     aaaTACCGGCTGTTTTTCCAGGCGACCAAAATAGAGGCCACAGCGACGAGCGCTGTCGACTTTATTCAACGGCGCATAATCTGCCAGCATCTg                    <  1:2945618/93‑1 (MQ=255)

                                                                     aaaTACCGGCTGTTTTTCCAGGCGACCAAAATAGAGGCCACAGCGACGAGCGCTGTCGACTTTATTCAACGGCGCATAATCTGCCAGCATCTg                    >  2:1302023/1‑93 (MQ=255)

                                                                     aaaTACCGGCTGTTTTTCCAGGCGACCAAAATAGAGGCCACAGCGACGAGCGCTGTCGACTTTATTCAACGGCGCATAATCTGCCAGCATCTg                    <  1:1990243/93‑1 (MQ=255)

                                                                      aaTACCGGCTGTTTTTCCAGGCGACCAAAATAGAGGCCACAGAGACGAGCGCTGTCGACTTTATTCAACGGCGCATAATCTGCCAGCATCTGc                   >  2:4687017/1‑93 (MQ=255)

                                                                        tACCGGCTGTTTTTCCAGGCGACCAAAATAGAGGCCACAGCGACGAGCGCTGTCGACTTTATTCAACGGCGCATAATCTGCCAGCATCTGCTc                 >  2:4873593/1‑93 (MQ=255)

                                                                         aCCGGCTGTTTTTCCAGGCGACCAAAATAGAGGCCACAGCGACGAGCGCTGTCGACTTTAATCAACGGCGCATAATCTGCCAGCATCTGCTCg                >  2:3883980/1‑93 (MQ=255)

                                                                             gCTGTTTTTCCAGGCGACCAAAATAGAGGCCACAGCGACGAGCGCTGTCGACTTTATTCAACGGCGCATAATCTGCCAGCATCTGCTCGgcgc            <  2:5279241/93‑1 (MQ=255)

                                                                               tGTTTTTCCAGGCGACCAAAATAGAGGCCACAGCGACGAGCGCTGTCGACTTTATTCAACGGCGCATAATCTGCCAGCATCTGCTCGGCGCTg          >  1:774160/1‑93 (MQ=255)

                                                                                 tttttCCAGGCGACCAAAATAGAGGCCACAGCGACGAGCGCTGTCGACTTTATTCAACGGCGCATAATCTGCCAGCATCTGCTCGGCGCTgcg        >  2:5268309/1‑93 (MQ=255)

                                                                                    ttCCAGGCGACCAAAATAGAGGCCACAGCGACGAGCGCTGTCGACTTTATTCAACGGCGCATAATCTGCCAGCATCTGCTCGGCGCTGCGCAc     <  2:6604527/93‑1 (MQ=255)

                                                                                     tCCAGGCGACCAAAATAGAGGCCACAGCGACGAGCGCTGTCGACTTTATTCAACGGCGCATAATCTGCCAGCATCTGCTCGGCGCTGCGCAcg    >  2:1378789/1‑93 (MQ=255)

                                                                                      ccAGGCGACCAAAATAGAGGCCACAGCGACGAGCGCTGTCGACTTTATTCAACGGCGCATAATCTGCCAGCATCTGCTCGGCGCTGCGCAcgc   <  2:6049483/93‑1 (MQ=255)

                                                                                      ccAGGCGACAAAAATAGAGGCCACAGCGACGAGCGCTGTCGACTTTATTCAACGGCGCATAATCTGCCAGCATCTGCTCGGCGCTGCGCAcgc   <  1:521270/93‑1 (MQ=255)

                                                                                       cAGGCGACCAAAATAGAGGCCACAGCGACGAGCGCTGTCGACTTTATTCAACGGCGCATAATCTGCCAGCATCTGCTCGGCGCTGCGCAcgcg  >  2:2290145/1‑92 (MQ=255)

                                                                                       cAGGCGACCAAAATAGAGGCCACAGCGACGAGCGCTGTCGACTTTATTCAACGGCGCATAATCTGCCAGCATCTGCTCGGCGCTGCGCAcgcc  <  1:4503430/93‑1 (MQ=255)

                                                                                       cAGGCGACCAAAATAGAGGCCACAGCGACGAGCGCTGTCGACTTTATTCAACGGCGCATAATCTGCCAGCATCTGCTCGGCGCTGCGCAcgcc  >  2:2686780/1‑93 (MQ=255)

                                                                                       cAGGCGACCAAAATAGAGGCCACAGCGACGAGCGCTGTCGACTTTATTCAACGGCGCATAATCTGCCAGCATCTGCTCGGCGCTGCGCAcgcc  >  2:2486231/1‑93 (MQ=255)

                                                                                       cAGGCGACCAAAATAGAGGCCACAGCGACGAGCGCTGTCGACTTTATTCAACGGCGCATAATCTGCCAGCATCTGCTCGGCGCTGCGCAcgcc  >  1:5019320/1‑93 (MQ=255)

AAACACGCCAGGCAGCGTTTTGACCGTCAGGCCATCGACGCTGTATTCGCCCCAGAATTTCTCCGCATCAAATACCGGCTGTTTTTCCAGACGACCAAAATAGAGGCCACAGCGACGAGCGCTGTCGACTTTATTCAACGGCGCATAATCTGCCAGCATCTGCTCGGCGCTGCGCACGCC  >  NC_000913/4604742‑4604921

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 19 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`