BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	654,812	G→A	A127V (GCC→GTC)	dcuC ←	DcuC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	654,812	0	G	A	100.0%	866.3 / NA	234	A127V (GCC→GTC)	dcuC	DcuC
Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (110/124); total (110/124)

Only 100 of 255 total aligned reads displayed.

TACCAACGTTTACCATCACCGGAAATAGGGTTGCCATCAGCAAAACACCCAGACCGGTTGCGGAAGAGACGGCCAGAGACATCAGACAGGCGACAAAATAGGCGGCAATCATCAGCAGGTAAGGGGAGTTAATATACTGCAATGGTTTTGACGCCAGCTTGACCACCATATCATTCGCG  >  NC_000913/654723‑654901

tACCAACGTTTACCATCACCGGAAATAGGGTTGCCATCAGCAAAACACCCAGACCGGTTGCGGAAGAGACGGCCAGAGACATCAGACAGACGa                                                                                        >  1:5891508/1‑93 (MQ=255)

 aCCAACGTTTACCATCACCGGAAATAGGGTTGCCATCAGCAAAACACCCAGACCGGTTGCGGAAGAGACGGCCAGAGACATCAGACAgacgac                                                                                       >  1:1286421/1‑93 (MQ=255)

  ccAACGTTTACCATCACCGGAAATAGGGTTGCCATCAGCAAAACACCCAGACCGGTTGCGGAAGAGACGGCCAGAGACATCAGACAGACGACa                                                                                      >  1:3417076/1‑93 (MQ=255)

     aCGTTTACCATCACCGGAAATAGGGTTGCCATCAGCAAAACACCCAGACCGGTTGCGGAAGAGACGGCCAGAGACATCAGACAGACGACaaaa                                                                                   <  2:2662352/93‑1 (MQ=255)

     aCGTTTACCATCACCGGAAATAGGGTTGCCATCAGCAAAACACCCAGACCGGTTGCGGAAGAGACGGCCAGAGACATCAGACAGACGACaaaa                                                                                   <  1:858485/93‑1 (MQ=255)

      cGTTTACCATCACCGGAAATAGGGTTGCCATCAGCAAAACACCCAGACCGGTTGCGGAAGAGACGGCCAGAGACATCAGACAGACGACAAAAt                                                                                  >  2:3629878/1‑93 (MQ=255)

       gTTTACCATCACCGGAAATAGGGTTGCCATCAGCAAAACACCCAGACCGGTTGCGGAAGAGACGGCCAGAGACATCAGACAGACGACAAAATa                                                                                 <  1:3456544/93‑1 (MQ=255)

       gTTTACCATCACCGGAAATAGGGTTGCCATCAGCAAAACACCCAGACCGGTTGCGGAAGAGACGGCCAGAGACATCAGACAGACGACAAAATa                                                                                 >  1:2241122/1‑93 (MQ=255)

         ttACCATCACCGGAAATAGGGTTGCCATCAGCAAAACACCCAGACCGGTTGCGGAAGAGACGGCCAGAGACATCAGACAGACGACAAAATAgg                                                                               <  1:1427696/93‑1 (MQ=255)

          tACCATCACCGGAAATAGGGTTGCCATCAGCAAAACACCCAGACCGGTTGCGGAAGAGACGGCCAGAGACATAAGACAGACGACAAAATAggt                                                                              >  2:2046586/1‑92 (MQ=255)

           aCCATCACCGGAAATAGGGTTGCCATCAGCAAAACACCCAGACCGGTTGCGGAAGAGACGGCCAGAGACATCAGACAGACGACAAAATAggcg                                                                             >  2:1330973/1‑93 (MQ=255)

             cATCACCGGAAATAGGGTTGCCATCAGCAAAACACCCAGACCGGTTGCGGAAGAGACGGCCAGAGACATCAGACAGACGACAAAATAggcggc                                                                           >  2:233819/1‑93 (MQ=255)

             cATCACCGGAAATAGGGTTGCCATCAGCAAAACACCCAGACCGGTTGCGGAAGAGACGGCCAGAGACATCAGACAGACGACAAAATAggcggc                                                                           >  1:2088668/1‑93 (MQ=255)

             cATCACCGGAAATAGGGTTGCCATCAGCAAAACACCCAGACCGGTTGCGGAAGAGACGGCCAGAGACATCAGACAGACGACAAAATAggcggc                                                                           >  2:1479906/1‑93 (MQ=255)

             cATCACCGGAAATAGGGTTGCCATCAGCAAAACACCCAGACCGGTTGCGGAAGAGACGGCCAGAGACATCAGACAGACGACAAAATAggcggc                                                                           >  2:6071734/1‑93 (MQ=255)

                 aCCGGAAATAGGGTTGCCATCAGCAAAACACCCAGTCCGGTTGCGGAAGAGACGGCCAGAGACATCAGACAGACGACAAAATAGGCGGCAatc                                                                       <  1:4569122/93‑1 (MQ=255)

                 aCCGGAAATAGGGTTGCCATCAGCAAAACACCCAGACCGGTTGCGGAAGAGACGGCCAGAGACATCAGACAGACGACAAAATAGGCGGCAatc                                                                       <  2:364818/93‑1 (MQ=255)

                 aCCGGAAATAGGGTTGCCATCAGCAAAACACCCAGACCGGTTGCGGAAGAGACGGCCAGAGACATCAGACAGACGACAAAATAGGCGGCAatc                                                                       >  1:3023463/1‑93 (MQ=255)

                 aCCGGAAATAGGGTTGCCATCAGCAAAACACCCAGACCGGTTGCGGAAGAGACGGACAGAGACATCAGACAGACGACAAAATAGGCGGCAatc                                                                       >  2:3904367/1‑93 (MQ=255)

                  ccGGAAATAGGGTTGCCATCAGCAAAACACCCAGACCGGTTGCGGAAGGGACGGCCAGAGACATCAGACAGACGACAAAATAGGCGGCAatca                                                                      >  2:4478272/1‑93 (MQ=255)

                  ccGGAAATAGGGTTGCCATCAGCAAAACACCCAGACCGGTTGCGGAAGAGACGGCCAGAGACATCAGACAGACGACAAAATAGGCGGCAatca                                                                      <  1:1779850/93‑1 (MQ=255)

                    ggAAATAGGGTTGCCATCAGCAAAACACCCAGACCGGTTGCGGAAGAGACGGCCAGAGACATCAGACAGACGACAAAATAGGCGGCAatcatc                                                                    <  2:6617027/93‑1 (MQ=255)

                     gAAATAGGGTTGCCATCAGCAAAACACCCAGACCGGTTGCGGAAGAGACGGCCAGAGACATCAGACAGACGACAAAATAGGCGGCAAtcatca                                                                   >  2:346974/1‑93 (MQ=255)

                     gAAATAGGGTTGCCATCAGCAAAACACCCAGACCGGTTGCGGAAGAGACGGCCAGAGACATCAGACAGACGACAAAATAGGCGGCAAtcatca                                                                   <  2:641977/93‑1 (MQ=255)

                     gAAATAGGGTTGCCATCAGCAAAACACCCAGACCGGTTGCGGAAGAGACGGCCAGAGACATCAGACAGACGACAAAATAGGCGGCAAtcatca                                                                   <  2:2404046/93‑1 (MQ=255)

                      aaaTAGGGTTGCCATCAGCAAAACACCCAGACCGGTTGCGGAAGAGACGGCCAGAGACATCAGACAGACGACAAAATAGGCGGCAATCATcag                                                                  >  2:2364024/1‑93 (MQ=255)

                      aaaTAGGGTTGCCATCAGCAAAACACCCAGACCGGTTGCGGAAGAGACGGCCAGAGACATCAGACAGACGACAAAATAGGCGGCAATCATcag                                                                  <  1:2625648/93‑1 (MQ=255)

                      aaaTAGGGTTGCCATCAGCAAAACACCCAGACCGGTTGCGGAAGAGACGGCCAGAGACATCAGACAGACGACAAAATAGGCGGCAATCATcag                                                                  >  2:619941/1‑93 (MQ=255)

                       aaTAGGGTTGCCATCAGCAAAACACCCAGACCGGTTGCGGAAGAGACGGCCAGAGACATCAGACAGACGACAAAATAGGCGGCAATCATcagc                                                                 >  1:964950/1‑93 (MQ=255)

                        aTAGGGTTGCCATCAGCAAAACACCCAGACCGGTTGCGGAAGAGACGGCCAGAGACATCAGACAGACGACAAAATAGGCGGCAATCATcagca                                                                <  1:1002084/93‑1 (MQ=255)

                        aTAGGGTTGCCATCAGCAAAACACCCAGACCGGTTGCGGAAGAGACGGCCAGAGACATCAGACAGACGACAAAATAGGCGGCAATCATcagca                                                                >  1:1620092/1‑93 (MQ=255)

                         tAGGGTTGCCATCAGCAAAACACCCAGACCGGTTGCGGAAGAGACGGCCAGAGACATCAGACAGACGACAAAATAGGCGGCAATCATcagcag                                                               <  1:1468741/93‑1 (MQ=255)

                          aGGGTTGCCATCAGCAAAACACCCAGACCGGTTGCGGAAGAGACGGCCAGAGACATCAGACAGACGACAAAATAGGCGGCAATCATCAGCAgg                                                              <  2:4441943/93‑1 (MQ=255)

                          aGGGTTGCCATCAGCAAAACACCCAGACCGGTTGCGGAAGAGACGGCCAGAGACATCAGACAGACGACAAAATAGGCGGCAATCATCAGCAgg                                                              <  1:4332227/93‑1 (MQ=255)

                          aGGGTTGCCATCAGCAAAACACCCAGACCGGTTGCGGAAGAGACGGCCAGAGACATCAGACAGACGACAAAATAGGCGGCAATCATCAGCAgg                                                              >  2:4404516/1‑93 (MQ=255)

                            ggTTGCCATCAGCAAAACACCCAGACCGGTTGCGGAAGAGACGGCCAGAGACATCAGACAGACGACAAAATAGGCGGCAATCATCAGCAGGTa                                                            <  1:3651052/93‑1 (MQ=255)

                              ttGCGATCAGCAAAACACCCAGACCGGTTGCGGAAGAGACGGCCAGAGACATCAGTCAGACGACAAAATAGGCGGCAATCATCAGCAGGTAAg                                                          <  1:4764015/93‑1 (MQ=255)

                                gCCATCAGCAAAACACCCAGACCGGTTGCGGAAGAGACGGCCAGAGACATCAGACAGACGACAAAATAGGCGGCAATCATCAGCAGGTAAggg                                                        >  2:5001026/1‑93 (MQ=255)

                                gCCATCAGCAAAACACCCAGACCGGTTGCGGAAGAGACGGCCAGAGACATCAGACAGACGACAAAATAGGCGGCAATCATCAGCAGGTAAggg                                                        <  2:2474129/93‑1 (MQ=255)

                                gCCATCAGCAAAACACCCAGACCGGTTGCGGAAGAGACGGCCAGAGACATCAGACAGACGACAAAATAGGCGGCAATCATCAGCAGGTAAggg                                                        <  1:1944960/93‑1 (MQ=255)

                                 ccATCAGCAAAACACCCAGACCGGTTGCGGAAGAGACGGCCAGAGACATCAGACAGACGACAAAATAGGCGGCAATCATCAGCAGGTAAgggg                                                       <  2:3235786/93‑1 (MQ=255)

                                 ccATCAGCAAAACACCCAGACCGGTTGCGGAAGAGACGGCCAGAGACATCAGACAGACGACAAAATAGGCGGCAATCATCAGCAGGTAAgggg                                                       <  2:1079915/93‑1 (MQ=255)

                                   aTCAGCAAAACACCCAGACCGGTTGCGGAAGAGACGGCCAGAGACATCAGACAGACGACAAAATAGGCGGCAATCATCAGCAGGTAAGGGGAg                                                     >  1:6058394/1‑93 (MQ=255)

                                   aTCAGCAAAACACCCAGACCGGTTGCGGAAGAGACGGCCAGAGACATCAGACAGACGACAAAATAGGCGGCAATCATCAGCAGGTAAGGGGAg                                                     <  2:5261388/93‑1 (MQ=255)

                                    tCAGCAAACCACCCAGACCGGTTGCGGAAGAGACGGCCAGAGACATCAGACAGACGACAAAATAGGCGGCAATCATCAGCAGGTAAGGGGAGt                                                    <  1:2367840/93‑1 (MQ=255)

                                    tCAGCAAAACACCCAGACCGGTTGCGGAAGAGACGGCCAGAGACATCAGACAGACGACAAAATAGGCGGCAATCATCAGCAGGTAAGGGGAGt                                                    <  1:5956173/93‑1 (MQ=255)

                                      aGCAAAACACCCAGACCGGTTGCGGAAGAGACGGCCAGAGACATCAGACAGACGACAAAATAGGCGGCAATCATCAGCAGGTAAGGGGAGTTa                                                  >  2:1433319/1‑93 (MQ=255)

                                       gCAAAACACCCAGACCGGTTGCGGAAGAGACGGCCAGAGACATCAGACAGACGACAAAATAGGCGGCAATCATCAGCAGGTAAGGGGAGTTaa                                                 >  2:2923139/1‑93 (MQ=255)

                                        cAAAACACCCAGACCGGTTGCGGAAGAGACGGCCATAGACATCAGACAGACGACAAAATAGGCGGCAATCATCAGCAGGTAAGGGGAGTTAat                                                <  2:544678/93‑1 (MQ=255)

                                         aaaaCACCCAGACCGGTTGCGGAAGAGACGGCCAGAGACATCAGACAGACGACAAAATAGGCGGCAATCATCTGCAGGTAAGGGGAGTTAata                                               <  1:3784847/93‑1 (MQ=255)

                                          aaaCACCCAGACCGGTTGCGGAAGAGACGGCCAGAGACATCAGACAGACGACAAAATAGGCGGCAATCATCAGCAGGTAAGGGGAGTTAatat                                              <  1:2996870/93‑1 (MQ=255)

                                          aaaCACCCAGACCGGTTGCGGAAGAGACGGCCAGAGACATCAGACAGACGACAAAATAGGCGGCAATCATCAGCAGGTAAGGGGAGTTAatat                                              >  1:1768288/1‑93 (MQ=255)

                                          aaaCACCCAGACCGGTTGCGGAAGAGACGGCCAGAGACATCAGACAGAAGACAAAATAGGGGGAAATCATCAGGAGAGAGGGGGAGAAAatat                                              >  1:4409595/1‑93 (MQ=255)

                                           aaCACCCAGACCGGTTGCGGAAGAGACGGCCAGAGACATCAGACAGACGACAAAATAGGCGGCAATCATCAGCAGGTAAGGGGAGTTAAtata                                             <  2:6578557/93‑1 (MQ=255)

                                            acacCCAGACCGGTTGCGGAAGAGACGGCCAGAGACATCAGACAGACGACAAAATAGGCGGCAATCATCAGCAGGTAAGGGGAGTTAATATAc                                            <  2:4779245/93‑1 (MQ=255)

                                             cacCCAGACCGGTTGCGGAAGAGACTGCCAGAGACATCAGACAGACGACAAAATAGGCGGCAATCATCAGCAGGTAAGGGGAGTTAATATACt                                           <  1:3236557/93‑1 (MQ=255)

                                             cacCCAGACCGGTTGCGGAAGAGACGGCCAGAGACATCAGACAGACGACAAAATAGGCGGCAATCATCAGCAGGTAAGGGGAGTTAATATACt                                           >  2:3310978/1‑93 (MQ=255)

                                                 cAGACCGGTTGCGGAAGAGACGGCCAGAGACATCAGACAGACGACAAAATAGGCGGCAATCATCAGCAGGTAAGGGGAGTTAATATACTGCaa                                       >  1:4446947/1‑93 (MQ=255)

                                                  aGACCGGTTGCGGAAGAGACGGCCAGAGACATCAGACAGACGACAAAATAGGCGGCAATCATCAGCAGGTAAGGGGAGTTAATATACTGCAAt                                      >  2:3342576/1‑93 (MQ=255)

                                                  aGACCGGTTGCGGAAGAGACGGCCAGAGACATCAGACAGACGACAAAATAGGCGGCAATCATCAGCAGGTAAGGGGAGTTAATATACTGCAAt                                      <  1:2320752/93‑1 (MQ=255)

                                                  aGACCGGTTGCGGAAGAGACGGCCAGAGACATCAGACAGACGACAAAATAGGCGGCAATCATCAGCAGGTAAGGGGAGTTAATATACTGCAAt                                      <  1:4133912/93‑1 (MQ=255)

                                                  aGACCGGTTGCGGAAGAGACGGCCAGAGACATCAGACAGACGACAAAATAGGCGGCAATCATCAGCAGGTAAGGGGAGTTAATATACTGCAAt                                      >  2:2100308/1‑93 (MQ=255)

                                                   gACCGGTTGCGGAAGAGACGGCCAGAGACATCAGACAGACGACAAAATAGGCGGCAATCATCAGCAGGTAAGGGGAGTTAATATACTGCAATg                                     <  1:5812879/93‑1 (MQ=255)

                                                   gACCGGTTGCGGAAGAGACGGCCAGAGACATCAGACAGACGACAAAATAGGCGGCAATCATCAGCAGGTAAGGGGAGTTAATATACTGCAATg                                     >  2:2667346/1‑93 (MQ=255)

                                                   gACCGGTTGCGGAAGAGACGGCCAGAGACATCAGACAGACGACAAAATAGGCGGCAATCATCAGCAGGTAAGGGGAGCTAATATACTGCAATg                                     >  1:6433723/1‑93 (MQ=255)

                                                    aCCGGTTGCGGAAGAGACGGCCAGAGACATCAGACAGACGACAAAATAGGCGGCAATCATCAGCAGGTAAGGGGAGTTAATATACTGCAATgg                                    <  1:1117500/93‑1 (MQ=255)

                                                     ccGGTTGCGGAAGAGACGGCCAGAGACATCAGACAGACGACAAAATAGGCGGCAATCATCAGCAGGTAAGGGGAGTTAATATACTGCAATGGt                                   <  2:1639116/93‑1 (MQ=255)

                                                     ccGGTTGCGGAAGAGACGGCCAGAGACATCAGACAGACGACAAAATAGGCGGCAATCATCAGCAGGTAAGGGGAGTTAATATACTGCAATGGt                                   <  1:6669309/93‑1 (MQ=255)

                                                      cGGTTGCGGAAGAGACGGCCAGAGACATCAGACAGACGACAAAATAGGCGGCAATCATCAGCAGGTAAGGGGAGTTAATATACTGCAATGGtt                                  <  1:3892905/93‑1 (MQ=255)

                                                       ggTTGCGGAAGAGACGGCCAGAGACATCAGACAGACGACAAAATAGGCGGCAATCATTAGCAGGTAAGGGGAGTTAATATACTGCAATGGttt                                 >  1:6470433/1‑93 (MQ=255)

                                                       ggTTGCGGAAGAGACGGCCAGAGACATCAGACAGACGACAAAATAGGCGGCAATCATCAGCAGGTAAGGGGAGTTAATATACTGCAATGGttt                                 >  2:1868853/1‑93 (MQ=255)

                                                       ggTTGCGGAAGAGACGGCCAGAGACATCAGACAGACGACAAAATAGGCGGCAATCATCAGCAGGTAAGGGGAGTTAATATACTGCAATGGttt                                 >  1:6205023/1‑93 (MQ=255)

                                                        gTTGCGGAAGAGACGGCCAGAGACATCAGACAGACGACAAAATAGGCGGCAATCATCAGCAGGTAAGGGGAGTTAATATACTGCAATGGtttt                                <  1:6280109/93‑1 (MQ=255)

                                                        gTTGCGGAAGAGACGGCCAGAGACATCAGACAGACGACAAAATAGGCGGCAATCATCAGCAGGTAAGGGGAGTTAATATACTGCAATGGtttt                                >  1:5113094/1‑93 (MQ=255)

                                                        gTTGCGGAAGAGACGGCCAGAGACATCAGACAGACGACAAAATAGGCGGCAATCATCAGCAGGTAAGGGGAGTTAATATACTGCAATGGtttt                                <  1:4895809/93‑1 (MQ=255)

                                                         ttGCGGAAGAGACGGCCAGAGACATCAGACAGACGACAAAATAGGCGGCAATCATCAGCAGGTAAGGGGAGTTAATATACTGCAATGGTTTTg                               >  2:4297326/1‑93 (MQ=255)

                                                           gCGGAAGAGACGGCCAGAGACATCAGACAGACGACAAAATAGGCGGCAATCATCAGCAGGTAAGGGGAGTTAATATACTGCAATGGTTTTGAc                             <  2:5065997/93‑1 (MQ=255)

                                                             ggAAGAGACGGCCAGAGACATCAGACAGACGACAAAATAGGCGGCAATCATCAGCAGGTAAGGGGAGTTAATATACTGCAATGGTTTTGACGc                           <  2:318947/93‑1 (MQ=255)

                                                             ggAAGAGACGGCCAGAGACATCAGACAGACGACAAAATAGGCGGCAATCATCAGCAGGTAAGGGGAGTTAATATACTGCAATGGTTTTGACGc                           <  1:1573621/93‑1 (MQ=255)

                                                             ggAAGAGACGGCCAGAGACATCAGACAGACGACAAAATAGGCGGCAATCATCAGCAGGTAAGGGGAGTTAATATACTGCAATGGTTTTGACGc                           >  2:1279567/1‑93 (MQ=255)

                                                              gAAGAGACGGCCAGAGACATCAGACAGACGACAAAATAGGCGGCAATCATCAGCAGGTAAGGGGAGTTAATATACTGCAATGGTTTTGACGcc                          <  2:3396186/93‑1 (MQ=255)

                                                                   gaCGGCCAGAGACATCAGACAGACGACAAAATAGGCGGCAATCATCAGCAGGTAAGGGGAGTTAATATACTGCAATGGTTTTGACGCCAGCtt                     <  1:2096721/93‑1 (MQ=255)

                                                                    aCGGCCAGAGACATCAGACAGACGACAAAATAGGCGGCAATCATCAGCAGGTAAGGGGAGTTAATATACTGCAATGGTTTTGACGCCAGCTTg                    <  2:1818199/93‑1 (MQ=255)

                                                                     cGGCCAGAGACATCAGACAGACGACAAAATAGGCGGCAATCATCAGCAGGTAAGGGGAGTTAATATACTGCAATGGTTTTGACGCCAGCTTGa                   <  1:3116471/93‑1 (MQ=255)

                                                                         cAGAGACATCAGACAGACGACAAAATAGGCGGCAATCATCAGCAGGTAAGGGGAGTTAATATACTGCAATGGTTTTGACGCCAGCTTGaccac               <  2:1010584/93‑1 (MQ=255)

                                                                          agagACATCAGACAGACGACAAAATAGGCGGCAATCATCAGCAGGTAAGGGGAGTTAATATACTGCAATGGTTTTGACGCCAGCTTGaccacc              >  1:2568578/1‑93 (MQ=255)

                                                                           gagaCATCAGACAGACGACAAAATAGGCGGCAATCATCAGCAGGTAAGGGGAGTTAATATACTGCAATGGTTTTGACGCCAGCTTGAccacca             >  2:602471/1‑93 (MQ=255)

                                                                            agaCATCAGACAGACGACAAAATAGGCGGCAATCATCAGCAGGTAAGGGGAGTTAATATACTGCAATGGTTTTGACGCCAGCTTGACCACCat            >  1:5274443/1‑93 (MQ=255)

                                                                            agaCATCAGACAGACGACAAAATAGGCGGCAATCATCAGCAGGTAAGGGGAGTTAATATACTGCAATGGTTTTGACGCCAGCTTGACCACCat            >  1:5338807/1‑93 (MQ=255)

                                                                             gaCATCAGACAGACGACAAAATAGGAGGCAATCATCAGCAGGTAAGGGGAGTTAATATACTGCAATGGTTTTGACGCCAGCATGACCACCata           >  1:2187296/1‑93 (MQ=255)

                                                                               cATCAGACAGACGACAAAATATGCGGCAATCATCAGCAGGCAAGGGGAGTTAATATACTGCAATGGTTTTGACGCGAGGTTGACCTCCATATc         >  1:1183756/1‑93 (MQ=255)

                                                                               cATCAGACAGACGACAAAATAGGCGGCAATCATCAGCAGGTAAGGGGAGTTAATATACTGCAATGGTTTTGACGCCAGCTTGACCACCATATc         <  2:5725618/93‑1 (MQ=255)

                                                                                 tCAGACAGACGACAAAATAGGCGGCAATCATCAGCAGGTAAGGGGAGTTAATATACTGCAATGGTTTTGACGCCAGCTTGACCACCATATCAt       <  1:726814/93‑1 (MQ=255)

                                                                                  cagacagaCGACAAAATAGGCGGCAATCATCAGCAGGTAAGGGGAGTTAATATACTGCAATGGTTTTGACGCCAGCTTGACCACCATATCAtt      <  1:3548046/93‑1 (MQ=255)

                                                                                  cagacagaCGACAAAATAGGCGGCAATCATCAGCAGGTAAGGGGAGTTAATATACTGCAATGGTTTTGACGCCAGCTTGAACACCATATCAtt      >  2:877441/1‑93 (MQ=255)

                                                                                   agacagacGACAAAATAGGCGGCAATCATCAGCAGGTAAGGGGAGTTAATATACTGCAATGGTTTTGACGCCAGCTTGACCACCATATCATTc     >  2:5991129/1‑93 (MQ=255)

                                                                                   agacagacGACAAAATAGGCGGCAATCATCAGCAGGTAAGGGGAGTTAATATACTGCAATGGTTTTGACGCCAGCTTGACCACCATATCATTc     <  2:5200177/93‑1 (MQ=255)

                                                                                   agacagacGACAAAATAGGCGGCAATCATCAGCAGGTAAGGGGAGTTAATATACTGCAATGGTTTTGACGCCAGCTTGACCACCATATCATTc     <  1:6577523/93‑1 (MQ=255)

                                                                                   agacagacGACAAAATAGGCGGCAATCATCAGCAGGTAAGGGGAGTTAATATACTGCAATGGTTTTGACGCCAGCTTGACCACCATATCATTc     >  1:5698951/1‑93 (MQ=255)

                                                                                    gACAGACGACAAAATAGGCGGCAATCATCAGCAGGTAAGGGGAGTTAATATACTGCAATGGTTTTGACGCCAGCTTGACCACCATATCATTcg    <  2:4574729/93‑1 (MQ=255)

TACCAACGTTTACCATCACCGGAAATAGGGTTGCCATCAGCAAAACACCCAGACCGGTTGCGGAAGAGACGGCCAGAGACATCAGACAGGCGACAAAATAGGCGGCAATCATCAGCAGGTAAGGGGAGTTAATATACTGCAATGGTTTTGACGCCAGCTTGACCACCATATCATTCGCG  >  NC_000913/654723‑654901

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 20 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`